CN103330057A - Composite feed supplement capable of effectively improving feed utilization ratio and preparation thereof - Google Patents
Composite feed supplement capable of effectively improving feed utilization ratio and preparation thereof Download PDFInfo
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Abstract
The invention discloses a composite feed supplement capable of effectively improving the feed utilization ratio and preparation thereof and belongs to the field of feed supplements. The feed supplement comprises the following raw materials in parts by weight: 20-30 parts of radix asparagi powder, 20-35 parts of echinacea powder, 15-30 parts of cattail pollen powder, 20-30 parts of dogwood powder, 1-5 part (s) of cordyceps taishanensis powder, 1-5 part (s) of cordyceps sinensis fermentation liquor extractive, 2-8 parts of bacillus natto agent, 5-10 parts of radix asparagi composite extractive, 2-4 parts of Aspergillus awamori culture and 0.5-2 part (s) of licorice powder. The raw materials are respectively prepared by a certain methods and are uniformly mixed to obtain the composite feed supplement. The composite feed supplement can effectively improve the immunity and intestinal health of pigs, reduce diseases of pigs, ensure healthiness of pigs, promote growth and development, and simultaneously protect the feed against mildew and nutrient substances against oxidation loss, thereby improving the feed utilization ratio.
Description
Technical field:
The present invention relates to a kind of pig's feed additive, particularly a kind of composite feed additive of effective raising efficiency of feed utilization relates to the preparation method of this additive simultaneously, belongs to feed additive field
Background technology:
Feed is the prime cost of animal-breeding, and the animal-breeding cost has 60%-70% to come from feed cost usefulness according to statistics, therefore reduces feed waste, improves efficiency of feed utilization, is the key point of carrying out aquaculture cost control and improving culture benefit.At present the common efficiency of feed utilization that exists of aquaculture is not high, nutritional labeling digestion not exclusively, keeping is not good at causing the feed moisture absorption to go mouldy or problem such as infested, the existence of these problems has not only increased feeding cost greatly, and has caused the wasting of resources.How to address these problems, the control aquaculture cost, reducing the breed risk is the big bottleneck of one in the aquaculture fast development.Have many about improving the research of efficiency of feed utilization in recent years, use the chemical synthesis additive agent field but mainly concentrate on, the chemical synthesis additive can cause animal drug resistance, problem such as residual, harm humans health, also has a large amount of excretas simultaneously, as pernicious gases such as ammonia, hydrogen sulfide, contaminated environment.Chinese herbal feed additive is as the focus of emerging research, has advantages such as natural, nutrition, side effect is little.Develop Chinese herbal feed additive energetically to solving the antibiotic residue problem, boost productivity, develop green animal husbandry, satisfy people's food security demand, dwindle China's animal husbandry and developed country's gap, strengthen China's livestock products in the competitiveness of international market, have important economic implications and social benefit.Studies show that, many Chinese herbal medicines itself just have good external bacteriostasis, different with Western medicine is, Chinese herbal feed antibiotic health care agent noresidue, have no drug resistance, its mechanism of action not only is the inhibitory or killing effect to pathogenic microorganism, the more important thing is the adjustment to the body disease-resistant repair ability, namely improve anti-stress ability, raising body's immunity and the disease-resistant defensive enginery of body, thereby reach the purpose that improves breeding performonce fo animals and food utilization efficiency.
Chinese herbal medicine is some plants with given efficacy, each is powerful for these plants, for example the effectiveness of part plant is as follows: lucid asparagus: its medicinal part mainly is the piece root, have replenishing the vital essence and removing heat, moisturize the effect of promoting the production of body fluid, modern pharmacology studies show that it has good curative effect to symptoms such as pulmonary tuberculosis, bronchitis, diphtheria, pertussis, dry mouth and throats, also has antibacterial immunity and anti-oxidation function simultaneously.Fructus Corni: its flavor acid, puckery, tepor is returned the liver kidney channel.Contain the various active composition, comprise volatile ingredient, glucosides class and aglycon, tannin and organic acid etc.Fructus Corni water decoction can significantly raise mice serum hemolytic antibody and serum antibody IgG content.Carbohydrate in the Fructus Corni has the immunoreactive effect of obvious promotion.Cattail pollen: be the dry pollen of Typhaceae plant raupo cattail, typha orientalis, theory of traditional Chinese medical science thinks that this property of medicine flavor is sweet flat, returns liver, the heart, the spleen channel, the effect of tool analgesia stagnation resolvation.Echinacea: originate in a class Echinacea plant in North America and Canada south, its main active is polysaccharide, alkylamide compound and caffeic acid analog derivative.Studies show that different xylan and arabinose in the Echinacea can pass through to stimulate the activity of the lymphocytic propagation of monokaryon and macrophage, thereby have significant immunostimulation.Echinacea also has antibacterial and anti-inflammation functions, and its active component Cichoric acid has the immune anti-inflammatory properties of enhancing, can suppress hyaluronic acid, and protection collagen III is avoided degraded, and Escherichia coli are had the obvious suppression effect.
How effectively to utilize existing herbal raw material preparation can effectively improve the feed addictive of animal immunizing power and the compound utilization ratio of raising feed, the high value utilization that realizes feed is one and is conducive to the project that the feed industry develops in a healthy way.
Summary of the invention:
The objective of the invention is to develop a kind of is the feed addictive that can effectively improve efficiency of feed utilization of primary raw material with the Chinese herbal medicine, this feed addictive has the immunity that improves pig, regulate intestinal health and prevent going mouldy and the function of oxidation such as vitamin of feed, thereby reduce the generation of disease, guarantee health, improve efficiency of feed utilization.
Technical scheme of the present invention is as follows:
Described feed addictive is made up of the raw material of following parts by weight:
Tianmen Green bean noodle 20-30 part; Echinacea powder 20-35 part; Cattail pollen powder 15-30 part; Fructus Corni powder 20-30 part; Cordyceps 1-5 part; Chinese caterpillar fungus fermentation liquid extract 1-5 part; Bafillus natto microbial inoculum 2-8 part; Lucid asparagus composite extract 5-10 part; Aspergillus awamori culture 2-4 part; Licorice powder 0.5-2 part.
Described feed addictive preferably is made up of the raw material of following parts by weight:
Tianmen Green bean noodle 22-28 part; Echinacea powder 25-30 part; Cattail pollen powder 20-28 part; Fructus Corni powder 25-30 part; Cordyceps 1-4 part; Chinese caterpillar fungus fermentation liquid extract 1-5 part; Bafillus natto microbial inoculum 2-6 part; Lucid asparagus composite extract 5-8 part; Aspergillus awamori culture 2-4 part; Licorice powder 1-2 part.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
Described Echinacea powder, preparation method thereof is as follows: it is below 1 millimeter that Echinacea is crushed to particle diameter through pulverizer;
Described cattail pollen powder, preparation method thereof is as follows: it is below 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
Described Fructus Corni powder, preparation method thereof is as follows: it is below 1 millimeter that Fructus Corni is crushed to particle diameter through pulverizer;
Described licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
Described cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step to obtain zymotic fluid, and the zymotic fluid centrifugation obtains mycelia, and the mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0.
Described cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, the zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 70-85 ℃ and keeps after 1-2 hour, fermented liquid spray drying is pulverized obtained the Chinese caterpillar fungus fermentation liquid extract.
Described Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0; Percentage by weight.
Described bafillus natto bacterial preparation process:
Cultivate bafillus natto from the inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in the fermentation tank; the control temperature is 19-24 ℃; the centrifugation acquisition wet thallus that finishes that fermented in 28-36 hour is cultivated in ventilation; add the protective agent freeze drying and obtain microbial inoculum, protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, all the other are water.
Described lucid asparagus composite extract preparation method is as follows:
Take by weighing lucid asparagus 50-75 part; Echinacea powder 10-15 part; Fructus Corni powder 8-15 part; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, in container, evenly mix and add the water of 3-6 times of weight then, the control temperature keeps 2-4h for 40 ℃-60 ℃, rising temperature to 80 ℃-85 ℃ of maintenance 3-5h, add the mixture of 2-3 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃-20 ℃ of maintenance 4-8h filter; The filtrate vacuum concentrates postlyophilization and obtains the lucid asparagus composite extract.The mass ratio of described butanols and propyl alcohol is 1:(0.3-0.6).Adopt the content of polysaccharide in the method gained lucid asparagus composite extract to reach 78-90%.
The preparation method of described aspergillus awamori culture is as follows:
After the slant strains activation culture is transferred to and cultivates the acquisition seed step by step on the slant medium, seed added mix the back in fermentation vat that sterilising medium is housed or the pallet and cultivate, the control of bent material cultivation temperature is at 18-26 ℃, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; Bent material culture technique commonly used is adopted in the cultivation of solid koji material; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through the thermophilic digestion sterilization treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time.
Drying and crushing: the fermentation ends compost carries out drying at fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and the material powder particle diameter is more than 60 orders.
Culture medium is formed: the solid material parts by weight consist of: wheat bran 70, soya-bean cake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
The preparation method of feed addictive of the present invention is as follows:
According to the raw material composition Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed and get final product.
The addition of product of the present invention in the live pig daily ration is 500-1000 gram/ton.
Beneficial effect:
The compatibility of above Chinese herbal medicine uses, and can effectively improve the immunity of pig and improve intestinal health, reduces the generation of pig disease, guarantee the health of pig, promotion is grown, and improves efficiency of feed utilization, and the while can prevent the oxidational losses of going mouldy of feed and nutriment.Use product of the present invention that pig immunity is improved a lot, antibiotic dosage reduces more than 60%, and pig goods taste and meat have obvious lifting, and the organoleptic test evaluation shows that estimating branch improves more than 65%.
Technique scheme of the present invention has the following advantages compared to existing technology:
The present invention is composite by science, Chinese caterpillar fungus fermentation liquid extract, lucid asparagus composite extract, Chinese caterpillar fungus powder and bafillus natto preparation etc. have been realized organic assembling, the traditional Chinese medicine ingredients and the bafillus natto preparation that have natural antibacterial and improve immunity have particularly been added, effectively reduce the use amount of antibiotics in letting animals feed, Cordyceps Militaris in incubation effectively absorption and accumulation the active component of lucid asparagus, improved the biological effect of product of the present invention.Lucid asparagus composite extract of the present invention contain polysaccharide, triterpene compound, glucoside, flavones, etc. bioactivator, safety non-toxic, has antibiotic, antiviral, as to strengthen immunity of organisms and the growth of promotion pig effect, can effectively improve food utilization efficiency, strengthen the immunity of pig, improve raise benefit, the security that can improve the animal flesh goods simultaneously.
The specific embodiment
Form and technology below by specific embodiment narration product of the present invention.Unless stated otherwise, used technological means is method known in those skilled in the art among the present invention.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes or change that separation and Extraction condition in these embodiments is carried out also belong to protection scope of the present invention.
The preparation of bafillus natto microbial inoculum:
The access of bafillus natto slant strains is shaken in the bottle, cultivate step by step and obtain seed liquid, it is 1.5 tons of secondary seed jars that seed liquid is inserted total measurement (volume) with 10% inoculum concentration, 1 ton of fermentation medium loading amount, 24 ℃ of condition of culture cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05MPa, incubation time 30 hours is cultivated and is finished cell concentration 5.0 * 10
8Individual/ml.Centrifugation: adopt centrifuge separation and fermentation liquid to obtain thalline.Adopt vacuum freeze-drying technique to handle the acquisition dry bacteria after adding protective agent; Protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, and all the other are water.
Fermentation medium is formed: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO
31%, all the other are pure water, pH6.8.
Number of viable is 2-8*10 in the described bafillus natto freeze-dried vaccine powder
8Individual/g.
Bacterial classification used herein is: bafillus natto (Bacillus natto), strain number is CICC10023.
The cordyceps preparation method:
The access of Cordyceps sinensis slant strains is shaken in the bottle, cultivate step by step and obtain seed liquid, inserting total measurement (volume) with 10% inoculum concentration is 5 tons fermentation tank, 3 tons of fermentation medium loading amounts, 24 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.4, tank pressure 0.05MPa, incubation time 72 hours.The zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, and fermented liquid is heated to 80 ℃ and keeps after 2 hours, fermented liquid spray drying is pulverized obtained the Chinese caterpillar fungus fermentation liquid extract.
Xu Jin hall volume " Chinese medicinal fungi " is seen in the cultivation of Cordyceps sinensis fungi liquid submerged fermentation liquid among the present invention.
Slant medium consists of: percentage by weight, and potato (liquor) 20%, glucose 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, agar 2.0%, the insufficient section pure water is supplied.
The seed culture medium composition is: percentage by weight, and sucrose 5%, glucose 2%, peptone 0.4%, soyabean protein powder 0.6%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0.
Described Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0; Percentage by weight.
The used Cordyceps sinensis of the present invention (Cordyceps sinensis) CICC50002 purchases in Chinese industrial microorganism fungus kind preservation center.
Embodiment 1
Described feed addictive preferably is made up of the raw material of following parts by weight:
25 parts of Tianmen Green bean noodles; 27 parts in Echinacea powder; 25 parts in cattail pollen powder; 27 parts in Fructus Corni powder, 2 parts of cordyceps; 3 parts of Chinese caterpillar fungus fermentation liquid extracts; 4 parts of bafillus natto microbial inoculums, 6 parts of lucid asparagus composite extracts, 3 parts of aspergillus awamori cultures, 1.5 parts of licorice powders.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
The Echinacea powder, preparation method thereof is as follows: it is below 1 millimeter that Echinacea is crushed to particle diameter through pulverizer;
The cattail pollen powder, preparation method thereof is as follows: it is below 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
The Fructus Corni powder, preparation method thereof is as follows: it is below 1 millimeter that Fructus Corni is crushed to particle diameter through pulverizer;
The licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
The cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step to obtain zymotic fluid, and the zymotic fluid centrifugation obtains mycelia, and the mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6%, Fructus Corni 1%, peptone 0.4%, soyabean protein powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0.
The cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, the zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 70 ℃ and keeps after 1.5 hours, fermented liquid spray drying is pulverized obtained the Chinese caterpillar fungus fermentation liquid extract.
The preparation method of described bafillus natto microbial inoculum is as follows:
Cultivate bafillus natto from the inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in the fermentation tank; the control temperature is 19 ℃; the centrifugation acquisition wet thallus that finishes that fermented in 32 hours is cultivated in ventilation; add the protective agent freeze drying and obtain microbial inoculum; protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, and all the other are water.
Described bacillus natto to ferment culture medium consists of: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO31%, all the other are pure water, pH6.8.
The preparation method is as follows for the lucid asparagus composite extract:
Take by weighing 50 parts of lucid asparagus; 10 parts in Echinacea powder; 12 parts in Fructus Corni powder; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, evenly mixes and add the water of 4 times of weight then in container, and the control temperature keeps 3h for 50 ℃, rising temperature to 80 ℃ maintenance 4h, add the mixture of 2 times of weight butanols of mixed material and propyl alcohol, control temperature to 16 ℃ maintenance 6h filters; The filtrate vacuum concentrates postlyophilization and obtains the lucid asparagus composite extract.The mass ratio of described butanols and propyl alcohol is 1:0.3.Adopt the content of polysaccharide in the method gained lucid asparagus composite extract to reach 86%.
The preparation method of described aspergillus awamori culture is as follows:
Slant strains activation culture: the aspergillus awamori slant strains is transferred on the slant medium, cultivated 20 days for 20 ℃.
The solid first order seed is cultivated: picking aspergillus awamori slant strains inserts in 500 milliliters of triangular flasks that 100 gram culture mediums are housed carries out seed culture, cultivates for 23 ℃ to get final product in 3 days.
The solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred to adding in the triangular flask that sterilising medium is housed behind the fragment carry out seed culture, condition of culture: 22 ℃ of cultivations got final product in 3 days.
Solid fermentation is cultivated: the secondary shake-flask seed is pulverized, added and mix the back in fermentation vat that sterilising medium is housed or the pallet and cultivate, the control of bent material cultivation temperature is at 22 ℃, humidity 80%, every 8 hours stirrings once, incubation time 6 days; Bent material culture technique commonly used is adopted in the cultivation of solid koji material; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through the thermophilic digestion sterilization treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time.
Drying and crushing: the fermentation ends compost carries out drying at fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and the material powder particle diameter is more than 60 orders.
Culture medium is formed: the solid material parts by weight consist of: wheat bran 70, soya-bean cake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
The preparation method of feed addictive of the present invention is as follows:
According to the raw material composition Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed granulation and get final product.
The addition of this product in the live pig daily ration is 800 grams/ton.
Embodiment 2
Described feed addictive preferably is made up of the raw material of following parts by weight:
22 parts of Tianmen Green bean noodles; 25 parts in Echinacea powder; 20 parts in cattail pollen powder; 25 parts in Fructus Corni powder, 1 part of cordyceps; 1 part of Chinese caterpillar fungus fermentation liquid extract; 2 parts of bafillus natto microbial inoculums, 5 parts of lucid asparagus composite extracts, 2 parts of aspergillus awamori cultures, 1 part of licorice powder.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
The Echinacea powder, preparation method thereof is as follows: it is below 1 millimeter that Echinacea is crushed to particle diameter through pulverizer;
The cattail pollen powder, preparation method thereof is as follows: it is below 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
The Fructus Corni powder, preparation method thereof is as follows: it is below 1 millimeter that Fructus Corni is crushed to particle diameter through pulverizer;
The licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
The cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step to obtain zymotic fluid, and the zymotic fluid centrifugation obtains mycelia, and the mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6%, Fructus Corni 0.5%, peptone 0.4%, soyabean protein powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0.
The cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, the zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 80 ℃ and keeps after 1.5 hours, fermented liquid spray drying is pulverized obtained the Chinese caterpillar fungus fermentation liquid extract.Experiment detects and shows that polyoses content has improved 25-40% than the polyoses content of non-heat treated in the Chinese caterpillar fungus fermentation liquid extract that obtains after the fermented liquid heat treated.
The preparation method of described bafillus natto microbial inoculum is as follows:
Cultivate bafillus natto from the inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in the fermentation tank; the control temperature is 19 ℃; the centrifugation acquisition wet thallus that finishes that fermented in 28 hours is cultivated in ventilation; add the protective agent freeze drying and obtain microbial inoculum, protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5 trehaloses.
Described bacillus natto to ferment culture medium consists of: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO31%, all the other are pure water, pH6.8.
The preparation method is as follows for the lucid asparagus composite extract:
Take by weighing 50 parts of lucid asparagus; 10 parts in Echinacea powder; 8 parts in Fructus Corni powder; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, evenly mixes and add the water of 3 times of weight then in container, and the control temperature keeps 2h for 40 ℃, rising temperature to 80 ℃ maintenance 3h, add the mixture of 2 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃ maintenance 4h filters; The filtrate vacuum concentrates postlyophilization and obtains the lucid asparagus composite extract.The mass ratio of described butanols and propyl alcohol is 1:0.5.Adopt the content of polysaccharide in the method gained lucid asparagus composite extract to reach 80%.
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: the aspergillus awamori slant strains is transferred on the slant medium, cultivated 2 days for 18 ℃.
The solid first order seed is cultivated: picking aspergillus awamori slant strains inserts in 500 milliliters of triangular flasks that 100 gram culture mediums are housed carries out seed culture, cultivates for 18 ℃ to get final product in 3 days.
The solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred to adding in the triangular flask that sterilising medium is housed behind the fragment carry out seed culture, condition of culture: 18 ℃ of cultivations got final product in 3 days.
Solid fermentation is cultivated: the secondary shake-flask seed is pulverized, added and mix the back in fermentation vat that sterilising medium is housed or the pallet and cultivate, the control of bent material cultivation temperature is at 18 ℃, humidity 82%, every 8 hours stirrings once, incubation time 5 days; Bent material culture technique commonly used is adopted in the cultivation of solid koji material; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through the thermophilic digestion sterilization treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time.
Drying and crushing: the fermentation ends compost carries out drying at fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and the material powder particle diameter is more than 60 orders.
Culture medium is formed: the solid material parts by weight consist of: wheat bran 70, soya-bean cake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
The preparation method of feed addictive of the present invention is as follows:
According to the raw material composition Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed granulation and get final product.
The addition of this product in the live pig daily ration is 500 grams/ton.
Embodiment 3
Described feed addictive preferably is made up of the raw material of following parts by weight:
28 parts of Tianmen Green bean noodles; 30 parts in Echinacea powder; 28 parts in cattail pollen powder; 30 parts in Fructus Corni powder, 4 parts of cordyceps; 5 parts of Chinese caterpillar fungus fermentation liquid extracts; 6 parts of bafillus natto microbial inoculums, 8 parts of lucid asparagus composite extracts, 4 parts of aspergillus awamori cultures, licorice powder 2%.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
The Echinacea powder, preparation method thereof is as follows: it is below 1 millimeter that Echinacea is crushed to particle diameter through pulverizer;
The cattail pollen powder, preparation method thereof is as follows: it is below 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
The Fructus Corni powder, preparation method thereof is as follows: it is below 1 millimeter that Fructus Corni is crushed to particle diameter through pulverizer;
The licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
The cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step to obtain zymotic fluid, and the zymotic fluid centrifugation obtains mycelia, and the mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 1%, Fructus Corni 1%, peptone 0.4%, soyabean protein powder 1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0.
The cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, the zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 85 ℃ and keeps after 2 hours, fermented liquid spray drying is pulverized obtained the Chinese caterpillar fungus fermentation liquid extract.
The preparation method of described bafillus natto microbial inoculum is as follows:
Cultivate bafillus natto from the inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in the fermentation tank; the control temperature is 24 ℃; the centrifugation acquisition wet thallus that finishes that fermented in 36 hours is cultivated in ventilation; add the protective agent freeze drying and obtain microbial inoculum; protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, and all the other are water.
Described bacillus natto to ferment culture medium consists of: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO31%, all the other are pure water, pH6.8.
The preparation method is as follows for the lucid asparagus composite extract:
Take by weighing 75 parts of lucid asparagus; 15 parts in Echinacea powder; 15 parts in Fructus Corni powder; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, evenly mixes and add the water of 6 times of weight then in container, and the control temperature keeps 4h for 60 ℃, rising temperature to 85 ℃ maintenance 5h, add the mixture of 3 times of weight butanols of mixed material and propyl alcohol, control temperature to 20 ℃ maintenance 8h filters; The filtrate vacuum concentrates postlyophilization and obtains the lucid asparagus composite extract.Adopt the content of polysaccharide in the method gained lucid asparagus composite extract to reach 90%.
The mass ratio of described butanols and propyl alcohol is 1:0.6.
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: the aspergillus awamori slant strains is transferred on the slant medium, cultivated 3 days for 25 ℃.
The solid first order seed is cultivated: picking aspergillus awamori slant strains inserts in 500 milliliters of triangular flasks that 100 gram culture mediums are housed carries out seed culture, cultivates for 26 ℃ to get final product in 3 days.
The solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred to adding in the triangular flask that sterilising medium is housed behind the fragment carry out seed culture, condition of culture: 26 ℃ of cultivations got final product in 3 days.
Solid fermentation is cultivated: the secondary shake-flask seed is pulverized, added and mix the back in fermentation vat that sterilising medium is housed or the pallet and cultivate, the control of bent material cultivation temperature is at 26 ℃, humidity 85%, every 6 hours stirrings once, incubation time 8 days; Bent material culture technique commonly used is adopted in the cultivation of solid koji material; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through the thermophilic digestion sterilization treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time.
Drying and crushing: the fermentation ends compost carries out drying at fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and the material powder particle diameter is more than 60 orders.
Culture medium is formed: the solid material parts by weight consist of: wheat bran 70, soya-bean cake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
Aspergillus awamori (Aspergillus awamori) CICC2250 buying is from Chinese industrial microorganism fungus kind preservation administrative center.
The addition of this product in the live pig daily ration is 1000 grams/ton.
The result of use experiment
The result of use test of the embodiment of the invention 1 feed addictive in weanling pig:
It is 120 of the healthy sucking pigs of (11.16 ± 0.45) kg that experimental animal is selected Guangnan (Zhanjiang) subordinate plant of tame rich feed corporation,Ltd average weight, and it is not remarkable to analyze body weight difference by statistics.Adopt the single-factor Randomized Designs, with 120 healthy sucking pigs, by male and female half and half, be divided into 2 groups (control group and test group), every group of 6 repetitions, each repeats 10 pigs.Test group is added product of the present invention 800 grams/ton that embodiment 1 makes, and control group does not add product of the present invention.Fed continuously 25 days, and compared with control group, use the invention feed addictive can obtain following effect:
| Project | Test group | Control group | Deviation |
| Starting weight (kg) | 11.08 | 11.24 | —— |
| End heavy (kg) | 23.54 | 22.40 | 4.8% |
| Daily ingestion amount (g) | 758.6 | 750.1 | —— |
| Daily gain (g) | 498.2 | 446.5 | 10.4% |
| Feedstuff-meat ratio | 1.52 | 1.68 | -10.5% |
| Diarrhea rate (%) | 4.8 | 11.6% | -141.7% |
Result of the test shows: add product of the present invention in the sucking pig daily ration, can effectively improve the daily gain of sucking pig, reduce feedstuff-meat ratio, reduce diarrhoea simultaneously, the hair color of experiment pig is carried out sensory evaluation scores, the experimental group scoring reaches 8.9 fens, and control group is 6.5 minutes; Above-mentioned data declaration product of the present invention can effectively improve the utilization rate of feed, improves the immunity of sucking pig simultaneously, reduces diarrhoea.
Claims (8)
1. composite feed additive that effectively improves efficiency of feed utilization comprises the raw material components of following parts by weight: Tianmen Green bean noodle 20-30 part; Echinacea powder 20-35 part; Cattail pollen powder 15-30 part; Fructus Corni powder 20-30 part; Cordyceps 1-5 part; Chinese caterpillar fungus fermentation liquid extract 1-5 part; Bafillus natto microbial inoculum 2-8 part; Lucid asparagus composite extract 5-10 part; Aspergillus awamori culture 2-4 part; Licorice powder 0.5-2 part; Described cordyceps sinensis fermentation liquor method for preparing extractive is: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, the zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 70-85 ℃ and keeps after 1-2 hour, fermented liquid spray drying is pulverized obtained the Chinese caterpillar fungus fermentation liquid extract; Described Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0; Percentage by weight; Described lucid asparagus composite extract preparation method is as follows: take by weighing lucid asparagus 50-75 part; Echinacea powder 10-15 part; Fructus Corni powder 8-15 part; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, in container, evenly mix and add the water of 3-6 times of weight then, the control temperature keeps 2-4h for 40 ℃-60 ℃, rising temperature to 80 ℃-85 ℃ of maintenance 3-5h, add the mixture of 2-3 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃-20 ℃ of maintenance 4-8h filter; The filtrate vacuum concentrates postlyophilization and obtains the lucid asparagus composite extract;
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: the aspergillus awamori slant strains is transferred on the slant medium, cultivated 2-3 days for 18-25 ℃;
The solid first order seed is cultivated: picking aspergillus awamori slant strains inserts in 500 milliliters of triangular flasks that 100 gram culture mediums are housed carries out seed culture, cultivates for 18-26 ℃ to get final product in 3 days;
The solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred to adding to be equipped with behind the fragment carry out seed culture in the triangular flask, condition of culture: 18-26 ℃ of cultivation got final product in 3 days;
Solid fermentation is cultivated: the secondary shake-flask seed is pulverized, added and mix the back in fermentation vat that sterilising medium is housed or the pallet and cultivate, the control of bent material cultivation temperature is at 18-26 ℃, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; Bent material culture technique commonly used is adopted in the cultivation of solid koji material; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through the thermophilic digestion sterilization treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time;
Drying and crushing: the fermentation ends compost carries out drying at fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and the material powder particle diameter is more than 60 orders;
Culture medium is formed: solid material: wheat bran, and 70%, soya-bean cake powder 6%, Tianmen Green bean noodle 5-8%, Fructus Corni powder 2-5%, cornstarch 5-8% adds the running water with solid matter equivalent; Initial pH nature.
2. composite feed additive that improves efficiency of feed utilization as claimed in claim 1 comprises the raw material components of following parts by weight: Tianmen Green bean noodle 22-28 part; Echinacea powder 25-30 part; Cattail pollen powder 20-28 part; Fructus Corni powder 25-30 part; Cordyceps 1-4 part; Chinese caterpillar fungus fermentation liquid extract 1-5 part; Bafillus natto microbial inoculum 2-6 part; Lucid asparagus composite extract 5-8 part; Aspergillus awamori culture 2-4 part; Licorice powder 1-2 part.
3. composite feed additive that improves efficiency of feed utilization as claimed in claim 1 or 2; it is characterized in that; the preparation method of described bafillus natto microbial inoculum is as follows: cultivate bafillus natto from the inclined-plane switching; seed liquor after spreading cultivation is step by step transferred in the fermentation tank; the control temperature is 19-24 ℃; the centrifugation acquisition wet thallus that finishes that fermented in 28-36 hour is cultivated in ventilation; add the protective agent freeze drying and obtain microbial inoculum, protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, all the other are water.
4. one kind as claim 1 or the 2 or 3 described composite feed additives that improve efficiency of feed utilization, it is characterized in that the mass ratio of described butanols and propyl alcohol is 1:0.3-0.6.
5. one kind prepares the preparation method who improves the composite feed additive of efficiency of feed utilization as claimed in claim 1 or 2:
According to the raw material composition Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed granulation and get final product;
The cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step to obtain zymotic fluid, and the zymotic fluid centrifugation obtains mycelia, and the mycelia drying and crushing obtains mycelium powder of sinensis;
Described Cordyceps Militaris fermentation medium consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6-1%, Fructus Corni 0.5-1%, peptone 0.4%, soyabean protein powder 0.5-1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0; Percentage by weight;
The cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, the zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 70-85 ℃ and keeps after 1-2 hour, fermented liquid spray drying is pulverized obtained the Chinese caterpillar fungus fermentation liquid extract;
The bafillus natto bacterial preparation process:
The preparation method of described bafillus natto microbial inoculum is as follows:
Cultivate bafillus natto from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in the fermentation tank, and the control temperature is 19-24 ℃, ventilates and cultivates 28-36 hour, and throughput is 2.0m
3/ minute; The fermentation centrifugation that finishes obtains wet thallus, adds the protective agent freeze drying and obtains microbial inoculum, and protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5 trehaloses;
Described lucid asparagus composite extract preparation method is as follows:
Take by weighing lucid asparagus 50-75 part; Echinacea powder 10-15 part; Fructus Corni powder 8-15 part; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, in container, evenly mix and add the water of 3-6 times of weight then, the control temperature keeps 2-4h for 40 ℃-60 ℃, rising temperature to 80 ℃-85 ℃ of maintenance 3-5h, add the mixture of 2-3 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃-20 ℃ of maintenance 4-8h filter; The filtrate vacuum concentrates postlyophilization and obtains the lucid asparagus composite extract;
The mass ratio of described butanols and propyl alcohol is 1:0.3-06;
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: the aspergillus awamori slant strains is transferred on the slant medium, cultivated 2-3 days for 18-25 ℃;
The solid first order seed is cultivated: picking aspergillus awamori slant strains inserts in 500 milliliters of triangular flasks that 100 gram culture mediums are housed carries out seed culture, cultivates for 18-26 ℃ to get final product in 3 days;
The solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred to adding to be equipped with behind the fragment carry out seed culture in the triangular flask, condition of culture: 18-26 ℃ of cultivation got final product in 3 days;
Solid fermentation is cultivated: the secondary shake-flask seed is pulverized, added and mix the back in fermentation vat that sterilising medium is housed or the pallet and cultivate, the control of bent material cultivation temperature is at 18-26 ℃, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; Bent material culture technique commonly used is adopted in the cultivation of solid koji material; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through the thermophilic digestion sterilization treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time;
Drying and crushing: the fermentation ends compost carries out drying at fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and material is pulverized the aperture more than 60 orders;
Culture medium is formed: solid material: wheat bran 70, soya-bean cake powder 6, Tianmen Green bean noodle 5-8, Fructus Corni powder 2-5, cornstarch 5-8; Add the running water with solid matter equivalent; Initial pH nature.
6. the preparation method of the composite feed additive of raising efficiency of feed utilization according to claim 5, described feed addictive is made up of the raw material of following parts by weight: 22 parts of Tianmen Green bean noodles; 25 parts in Echinacea powder; 20 parts in cattail pollen powder; 25 parts in Fructus Corni powder, 1 part of cordyceps; 1 part of Chinese caterpillar fungus fermentation liquid extract; 2 parts of bafillus natto microbial inoculums, 5 parts of lucid asparagus composite extracts, 2 parts of aspergillus awamori cultures, 1 part of licorice powder;
The preparation method is as follows:
According to the raw material composition Tianmen Green bean noodle, Echinacea powder, cattail pollen powder, Fructus Corni powder, cordyceps, Chinese caterpillar fungus fermentation liquid extract, bafillus natto microbial inoculum, lucid asparagus composite extract, aspergillus awamori culture, licorice powder are mixed granulation and get final product;
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 0.6%, Fructus Corni 0.5%, peptone 0.4%, soyabean protein powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, the insufficient section pure water is supplied, pH6.0;
The cordyceps sinensis fermentation liquor method for preparing extractive: cordyceps species obtains seed liquor through inoculation fermentation, cultivate step by step and obtain zymotic fluid, the zymotic fluid centrifugation is removed mycelium and is obtained fermented liquid, fermented liquid is heated to 80 ℃ and keeps after 1.5 hours, fermented liquid spray drying is pulverized obtained the Chinese caterpillar fungus fermentation liquid extract;
Described bacillus natto to ferment culture medium consists of: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO31%, all the other are pure water, pH6.8;
The preparation method is as follows for the lucid asparagus composite extract:
Take by weighing 50 parts of lucid asparagus; 10 parts in Echinacea powder; 8 parts in Fructus Corni powder; Respectively above-mentioned raw materials being crushed to particle diameter is below 2 millimeters, in container, evenly mix and add the water of 3-6 times of weight then, the control temperature keeps 2h for 40 ℃, rising temperature to 80 ℃ maintenance 3h, add the mixture of 2 times of weight butanols of mixed material and propyl alcohol, control temperature to 15 ℃ maintenance 4h filters; The filtrate vacuum concentrates postlyophilization and obtains the lucid asparagus composite extract; The mass ratio of described butanols and propyl alcohol is 1:0.5;
The preparation method of aspergillus awamori culture is as follows:
Slant strains activation culture: the aspergillus awamori slant strains is transferred on the slant medium, cultivated 2 days for 18 ℃;
The solid first order seed is cultivated: picking aspergillus awamori slant strains inserts in 500 milliliters of triangular flasks that 100 gram culture mediums are housed carries out seed culture, cultivates for 18 ℃ to get final product in 3 days;
The solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred to adding to be equipped with behind the fragment carry out seed culture in the triangular flask, condition of culture: 18 ℃ of cultivations got final product in 3 days;
Solid fermentation is cultivated: the secondary shake-flask seed is pulverized, added and mix the back in fermentation vat that sterilising medium is housed or the pallet and cultivate, the control of bent material cultivation temperature is at 18 ℃, humidity 82%, every 8 hours stirrings once, incubation time 5 days; Bent material culture technique commonly used is adopted in the cultivation of solid koji material; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through the thermophilic digestion sterilization treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time;
Drying and crushing: the fermentation ends compost carries out drying at fluid bed or other drying equipments, and baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and material is pulverized the aperture more than 60 orders.
7. according to the preparation method of the composite feed additive of claim 5 or 6 described raising efficiencies of feed utilization, the aspergillus awamori culture medium consists of, solid material: wheat bran, 70%, soya-bean cake powder 6%, Tianmen Green bean noodle 6%, Fructus Corni powder 3%, cornstarch 6% adds the running water with solid matter equivalent; Initial pH nature.
8. the application in the live pig daily ration of the composite feed additive that can improve efficiency of feed utilization as claimed in claim 1 or 2, the described addition of composite feed additive in the live pig daily ration that improves efficiency of feed utilization are 500-1000 gram/ton.
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| CN105982952B (en) * | 2015-03-02 | 2021-04-23 | 北京大北农动物保健科技有限责任公司 | A pharmaceutical composition, ZIGUANBAOGEN, comprises premix, concentrate and batch |
| CN105982952A (en) * | 2015-03-02 | 2016-10-05 | 北京大北农动物保健科技有限责任公司 | Echinacea herb, cyrtomium rhizome, cacumen platycladi and radix sophorae tonkinensis pharmaceutical composition, premix, concentrate feed and mixed batch |
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Effective date of registration: 20170207 Address after: 338099 Xinyu high tech Industrial Development Zone, Xinyu, Jiangxi Patentee after: Xinyu twin Feed Co., Ltd. Address before: 524045 Guangdong Province, Zhanjiang city Chikan District North Road No. 2 Patentee before: Guangnan (Zhanjiang) Jiafeng Feed Co.,Ltd. |
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Effective date of registration: 20191121 Address after: 530221 No.4, Liangling Road, Liangqing District, Nanning City, Guangxi Zhuang Autonomous Region Patentee after: Nanning HongPai feed Technology Co., Ltd Address before: 338099 Xinyu high tech Industrial Development Zone, Xinyu, Jiangxi Patentee before: Xinyu twin Feed Co., Ltd. |
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