CN104905016A - A feed additive and a preparation method thereof - Google Patents
A feed additive and a preparation method thereof Download PDFInfo
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Abstract
The present invention discloses a feed additive and a preparation method thereof which belongs to the field of feed additives. The feed additive consists of the following raw materials in parts by weight: 5-8 parts of asparagus cochinchinensis powder, 3-6 parts of typha angustifolia powder, 1-3 parts of ganoderma lucidum sporocarp powder, 2-6 parts of bacillus subtilis microbial inoculum, 2 -6 parts of angelica sinensis powder, 2-4 parts of aspergillus niger culture and 1-2 parts of glycyrrhiza uralensis powder. The feed additive is safe and non-toxic and has the antibacterial and anti-viral effects; the scientific compounding of the traditional Chinese medicine materials and the bacillus subtilis preparation achieves an organic combination, and thus the feed additive can effectively improve immunity and intestinal health of animals, reduce the occurrence of animal diseases, ensure the health and promote growth and development of animals, enhance the utilization rate of feeds, and at the same time prevent the mildewing of feeds and the oxidation and loss of nutrients. The feed additive greatly improves the immune system of pigs fed by the product, decreases antibiotic consumption by more than 60%, significantly improves the taste and meat quality of pork products, and increases the sensory experimental evaluation score by more than 67%.
Description
Technical field:
The present invention relates to a kind of feed addictive, belong to feed additive field.
Background technology:
At present, Chinese herbal feed additive, as the focus of emerging research, has the advantages such as natural, nutrition, side effect be little.Devoting Major Efforts To Developing Chinese herbal feed additive is to solution antibiotic residue problem, boost productivity, development unpolluted animal husbandry, meet the food security demand of people, reduce China's animal husbandry and developed country's gap, strengthen the competitiveness of China's livestock products in international market, there is important economic implications and social benefit.Research shows, many Chinese herbal medicines inherently have good In Vitro Bacteriostasis ability, with Western medicine unlike, Chinese herbal feed antibiotic health care agent noresidue, to have no drug resistance, its mechanism of action is not only the inhibitory or killing effect to pathogenic microorganism, the more important thing is the adjustment to body disease-resistant repair ability, namely improve the anti-stress ability of body, improve body's immunity and Defense response function, thus reach the object improving breeding performonce fo animals and food utilization efficiency.
Chinese herbal medicine is some plants with given efficacy, these plants are each powerful, the effectiveness of such as Activities of Some Plants is as follows: lucid asparagus: its medicinal part is block root mainly, there is replenishing the vital essence and removing heat, moisturize the effect of promoting the production of body fluid, modern pharmacology research shows that it has good curative effect to symptoms such as pulmonary tuberculosis, bronchitis, diphtheria, pertussis, dry mouth and throats, also has antibacterial immunity and anti-oxidation function simultaneously; Cattail pollen: be the dry pollen of Typhaceae plant raupo cattail, typha orientalis, theory of traditional Chinese medical science thinks that this property of medicine taste is sweet flat, returns liver, the heart, the spleen channel, effect of tool analgesia stagnation resolvation; At present, the Chinese medicine these with given efficacy is added in animal feed additive and uses, such as, publication number is the patent of invention " Chinese herbal feed additive " of CN102793058A, disclose a kind of Chinese herb feed additive synthesized by red heart sweet, conyza blinii, Radix Isatidis, honeysuckle, the vine of multiflower knotweed, mainly utilize VC content in red heart sweet higher, the immunity of animal can be strengthened, therefore, certain prevent disease, the effect of health care can only be played, only have the continuous feeding of long-time continuous, just can play certain onset; And publication number is the patent of invention " a kind of Chinese herbal feed additive " of CN102763765A, disclose a kind of feed addictive obtained by more than ten kind of Chinese herbal medicines such as hawthorn, Divine Comedy, Radix Codonopsis, wilsonii, Radix Isatidis, Chinese cassia tree, tarragons, growth of animal can be promoted, improve animal survival rate; But it forms complexity, and the Chinese medicine related to is of a great variety, and cost is higher, and the effect obtained is relatively weak, and Long-Time Service easily causes feed waste, be not suitable for large-scale farming field and use.
Therefore, how effectively to utilize existing herbal raw material to prepare the feed addictive that effectively can improve animal immunizing power and efficiency of feed utilization, the high value realizing feed utilizes, and is a project being conducive to feed industry and developing in a healthy way.
Summary of the invention:
The object of the invention is to develop a kind of feed addictive.
Technical scheme of the present invention is as follows: described feed addictive is made up of the raw material of following parts by weight: Tianmen Green bean noodle 5-8 part; Cattail pollen powder 3-6 part; Ganoderma lucidum fruitbody powder 1-3 part; Bacillus subtilis microbial agent 2-6 part; Angelica powder 2-6 part; Aspergillus niger culture 2-4 part; Licorice powder 1-2 part.
Described lucid asparagus powder, preparation method thereof is as follows: it is less than 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
Described cattail pollen powder, preparation method thereof is as follows: it is less than 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
Described licorice powder preparation method is as follows: it is less than 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
Described angelica powder preparation method is as follows: it is less than 1 millimeter that Radix Angelicae Sinensis is crushed to particle diameter through pulverizer;
Ganoderma lucidum fruitbody powder, preparation method thereof, it is crushed to particle diameter through pulverizer is less than 1 millimeter;
The preparation method of described aspergillus niger culture is as follows:
After slant strains activation culture is transferred to and slant medium cultivates acquisition seed step by step, seed is added in the fermentation vat that sterilising medium is housed or pallet and mixes rear cultivation, bent material cultivation temperature controls at 18-26 DEG C, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat compost cover with mycelia can terminate cultivate, culture medium in advance through thermophilic digestion sterilization treatment, sterilising conditions control temperature 121 DEG C, 1 hour time.
Drying and crushing: fermentation ends compost carries out drying on fluid bed or other drying equipments, baking temperature controls, at 60 DEG C, to be dried to moisture below 10%, then to be pulverized by solid culture medium, and crushing material particle diameter is more than 60 orders.
Culture medium forms: 70 parts, wheat bran, beancake powder 6 parts, Tianmen Green bean noodle 5-8 part, Fructus Corni powder 2-5 part, cornstarch 5-8 part; Add the running water with solid matter equivalent; Initial pH nature.
Aspergillus niger provided by the invention (Aspergillus niger) Li-2013-03 takes turns mutagenesis screening by aspergillus niger (Aspergillus niger) Li-2010 of a strain cellulase-producing of Tianjin University of Technology's Laboratories Accession through nitrosoguanidine more, then test through fermenting property strain excellent, screening obtains Aspergillus niger strain (Aspergillus niger) Li-2013-03 producing high activity cellulase.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 15th, 2013 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode 100101), preserving number is CGMCC NO.7927.
Described bacillus subtilis is commercially available business microbial inoculum, and deposit number is CICC10153.
The preparation method of feed addictive of the present invention is as follows:
According to raw material composition, Tianmen Green bean noodle, cattail pollen powder, ganoderma lucidum fruitbody powder, uninteresting gemma bacillus agent, angelica powder, aspergillus niger culture, licorice powder are mixed and granulate.
The addition of product of the present invention in live pig daily ration is 500-1000 gram/ton.
Beneficial effect:
Product safety of the present invention, nontoxic, there is antibacterial, antiviral effect; By by composite for the science of Chinese medicine, bacillus preparation, achieve organic assembling, effectively can improve the immunity of animal and improve intestinal health, reduce the generation of Animal diseases, promote animal growth, improve efficiency of feed utilization, going mouldy and the oxidational losses of nutriment of feed can be prevented simultaneously.Use the pig of product of the present invention, immunity is improved a lot, and antibiotic dosage decreases more than 60%, and the taste of pork product and meat have obvious lifting, and organoleptic test evaluation shows to evaluate a point raising more than 67%.
Detailed description of the invention
Product of the present invention composition and technique is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out separation and Extraction condition in these embodiments or change also belong to protection scope of the present invention.
Embodiment 1
The preparation of bacillus subtilis microbial agent:
By in bacillus subtilis slant strains access shaking flask, cultivate step by step and obtain seed liquid, it is 1.5 tons of secondary seed tanks that seed liquid is accessed total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 1 ton, condition of culture cultivation temperature 24 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1: 0.5, tank pressure 0.05MPa, incubation time 30 hours, cultivates and terminates cell concentration 5.0 × 10
8individual/ml.Centrifugation: adopt centrifuge separation and fermentation liquid to obtain thalline.Add protective agent in the thalline obtained after, vacuum freeze-drying technique process is adopted to obtain dry bacteria; Protective agent consists of 8% defatted milk, 3% lactose, 2% glycerine, 1.5% trehalose, and all the other are water.
Fermentation medium forms: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO
31%, all the other are pure water, pH6.8.
In described bacillus subtilis freeze-dried vaccine powder, number of viable is (2-8) × 10
8individual/g.
Described bacillus subtilis (Bacillus subtilis), strain number is CICC10153.
The bacterial strain of the high activity cellulase of product provided by the invention is specially aspergillus niger (Aspergillusniger) Li-2013-03.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 15th, 2013 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode 100101), protecting minus sign is CGMCC NO.7927.
Aspergillus niger strain of the present invention (Aspergillus niger) Li-2013-03 (CGMCC No.7927) has following microbial characteristic:
1, morphological feature:
Aspergillus niger strain CGMCC No.7927, biology morphology is for comprising conidium, born of the same parents' stalk, top capsule, producing several parts such as born of the same parents' structure.Conidial head is spherical to Radiation, and diameter 150-450 μm, conidiophore betides matrix.Born of the same parents obstruct stem 1000-3000 (length) × 12-20 (diameter) μm, yellow or yellowish-brown, and wall is level and smooth; Spherical or the almost spherical of top capsule, diameter 45-75 μm, surface can be educated comprehensively; Produce born of the same parents' structure double-deck, metulae 10-20 (length) × 4.5-7.0 (diameter) μm, bottle stalk 6-10 (length) × 2.5-3.5 (diameter) μm; conidium is spherical or subsphaeroidal; diameter 3-4.5 μm, brown, wall is coarse.
2, feature is cultivated:
Bacterial strain grows rapidly on wort agar culture medium, and 28 DEG C of fermentations, 4 days spores can be paved with inclined-plane; Quality velvet shape or be slightly with cotton-shaped; Conidium structure is a large amount of, and brown-black, without diffusate; Bacterium colony reverse side yellowish.
3, physiological and biochemical property:
Aspergillus niger strain CGMCC No.7927 can grow in the carbon sources such as maize straw, straw, wood chip, potato, corn flour, soluble starch, molasses, optimal pH scope 5-6, optimum growth temperature scope 28-33 DEG C, the suitableeest product enzyme temperature range 28-30 DEG C.
The triage techniques route of Aspergillus niger strain CGMCC No.7927 of the present invention is: the preparation → mutagenic treatment → plate isolation → primary dcreening operation → multiple sieve → genetic stability of starting strain → inclined-plane cultivation → spore suspension measures → expand experiment (fermenting property mensuration).
By mutagenesis screening scheme, mutant strain step-sizing is eliminated, finally to strain excellent through fermenting property test screen, obtain a plant height and produce enzyme performance bacterial strain black-koji mould (Aspergillus niger) Li-2013-03, the circumscribed 1,4 beta-glucanase of the cellulase after 96 hours of fermenting, Endo-β-glucanase, beta-glucosidase and filter paper enzyme activity reach 620U/mL, 1289U/mL, 456U/mL and 732U/mL respectively.
28 DEG C of fermentations, 4 days diameter 75mm, zymotic fluid cellulase circumscribed 1,4 beta-glucanase, Endo-β-glucanase, beta-glucosidase and filter paper enzyme activity reach 620U/mL, 1289U/mL, 456U/mL and 732U/mL respectively, improve 9.21 times, 7.43 times, 8.15 times and 10.31 times than starting strain respectively.
Produce the screening technique of high activity cellulase bacterial strain, comprise the following steps:
1) inclined-plane cultivate: by original Aspergillus niger strain (Aspergillus niger) Li-2010 streak inoculation slant medium, 30 DEG C cultivate 2 ~ 3d, until mycelium ripe, produce a large amount of black spore.Described slant medium is composed as follows: 12OBrix brewer's wort 1000mL, pH value nature, 121 DEG C of sterilizing 20min;
2) spore suspension preparation (following steps all aseptically operate): add 15mL sterilized water to test tube slant, spore is scraped, with Filter paper filtering, filtered solution poured into sterilizing and be added with in the 150mL triangular flask of 5-10 grain sterile glass beads, triangular flask is put into shaking table vibration 10-15min, spore is disperseed.
3) nitrosoguanidine (NTG) mutagenesis
1. with sterilized water spore suspension is adjusted to and is diluted to 10
6-10
7individual/mL.
2. get 10mL bacteria suspension to be transferred in 100mL triangular flask, add the NTG of 10mg, be mixed with the NTG solution that final concentration is 10mg/mL, and add 4-5 and drip acetone, be beneficial to NTG and dissolve.
3. at 30 DEG C, the centrifugal 10min of 200rpm oscillating reactions 30min, 5000rpm collects thalline, with SPSS washing several, and stopped reaction.
4. suitably spore concentration is adjusted to 10 by dilution
3individual/mL, get last dilution bacterium liquid 0.2mL, dilution spread is on cellulose-Congo red plate screening culture medium.The bacterial strain 200 that after cultivating 2 ~ 3 days at 30 DEG C, picking transparent circle/colony diameter is larger.(described cellulose-Congo red plate screening culture medium is composed as follows: cellulose powder 10g, Congo red 0.2g, ammonium sulfate 5g, magnesium sulfate 0.25g, potassium dihydrogen phosphate 1g, sodium chloride 0.1g, gelatin 2g, agar 20g, running water constant volume 1000mL, pH value 5-6,121 DEG C of sterilizing 20min).
5. sieve again: the 200 strain bacterium obtained are inoculated in slant medium with sterile toothpick, 30 respectively
dEG Cbe cultured to spore and be paved with inclined-plane.Respectively spore is equipped with 50mL and sieves again in the 250mL triangular flask of culture medium ferment to be inoculated under aseptic washing, inoculum concentration 10% (v/v), 30 DEG C, 100r/min cultivates 96h, measures the cellulase activity of each bacterial strain respectively.(the described culture medium of sieve is again composed as follows: cellulose powder 50g, ammonium sulfate 5g, magnesium sulfate 0.25g, potassium dihydrogen phosphate 1g, sodium chloride 0.1g, running water constant volume 1000mL, pH value 5-6,121 DEG C of sterilizing 20min).The bacterial strain choosing cellulose enzyme vigor the highest carries out amplification test.
4) genetic stability test
Li-2013-03 bacterial strain is gone down to posterity for continuous ten times on inclined-plane, and detects the fermentation situation after at every turn going down to posterity by the method that shaking flask is sieved again.Experiment finds, inclined-plane goes down to posterity for continuous ten times, and this bacterial classification proterties does not have significant change, and property indices is all normal, illustrates that the genetic stability of this bacterial classification is stronger thus.
5) scale-up
1. seed culture: bacterial strain (Aspergillus niger) Li-2013-03 the highest for cellulose enzyme vigor is accessed in 500mL triangular flask, seed culture medium loading amount 100 milliliters, 30 DEG C, 150rpm shaking table cultivation 72-96h.
2. seed tank culture: seed liquor is equipped with in the 10L fermentation tank of 7.5L zymotic fluid with the access of 10% (v/v) inoculum concentration, control ph is constant is 6.0 ± 0.2, cultivation temperature 30 ± 0.1 DEG C, mixing speed 300rpm, ventilation (v/v) 1: 0.8-1.2, incubation time 96h, dissolved oxygen 20-30%.Described fermentation medium consists of: cellulose powder 100g, ammonium sulfate 5g, magnesium sulfate 0.25g, potassium dihydrogen phosphate 1g, sodium chloride 0.1g, running water constant volume 1000mL, pH value 5-6,121 DEG C of sterilizing 20min.
After fermentation ends, get fermented supernatant fluid (crude enzyme liquid) and carry out enzyme activity detection, after measured, the circumscribed 1,4 beta-glucanase of bacterial strain (Aspergillus niger) Li-2013-03, Endo-β-glucanase, beta-glucosidase and filter paper enzyme activity reach 620U/mL, 1289U/mL, 456U/mL and 732U/mL respectively, improve 9.21 times, 7.43 times, 8.15 times and 10.31 times respectively than starting strain (Aspergillusniger) Li-2010.
Embodiment 2
Described feed addictive is preferably made up of the raw material of following parts by weight: Tianmen Green bean noodle 6 parts; 5 parts, cattail pollen powder; 2 parts, ganoderma lucidum fruitbody powder; Bacillus subtilis microbial agent 4 parts; Angelica powder 4 parts; Aspergillus niger culture 3 parts; Licorice powder 2 parts;
Described lucid asparagus powder, preparation method thereof is as follows: it is less than 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer;
Described cattail pollen powder, preparation method thereof is as follows: it is less than 1 millimeter that cattail pollen is crushed to particle diameter through pulverizer;
Described licorice powder preparation method is as follows: it is less than 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer;
Described angelica powder preparation method is as follows: it is less than 1 millimeter that Radix Angelicae Sinensis is crushed to particle diameter through pulverizer;
Ganoderma lucidum fruitbody powder, preparation method thereof, it is crushed to particle diameter through pulverizer is less than 1 millimeter;
The preparation method of described aspergillus niger culture is as follows:
After slant strains activation culture is transferred to and slant medium cultivates acquisition seed step by step, seed is added in the fermentation vat that sterilising medium is housed or pallet and mixes rear cultivation, bent material cultivation temperature controls at 24-26 DEG C, humidity 83-85%, every 8-9 hour stirring once, incubation time 6-7 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat compost cover with mycelia can terminate cultivate, culture medium in advance through thermophilic digestion sterilization treatment, sterilising conditions control temperature 121 DEG C, 1 hour time.
Drying and crushing: fermentation ends compost carries out drying on fluid bed or other drying equipments, baking temperature controls, at 60 DEG C, to be dried to moisture below 10%, then to be pulverized by solid culture medium, and crushing material particle diameter is more than 60 orders.
Culture medium forms: 70 parts, wheat bran, beancake powder 6 parts, Tianmen Green bean noodle 6 parts, 4 parts, Fructus Corni powder, cornstarch 6 parts; Add the running water with solid matter equivalent; Initial pH nature.
The preparation method of feed addictive of the present invention is as follows:
According to raw material composition, Tianmen Green bean noodle, cattail pollen powder, ganoderma lucidum fruitbody powder, uninteresting gemma bacillus agent, angelica powder, aspergillus niger culture, licorice powder are mixed and granulate.
The addition of this product in live pig daily ration is 800 grams/ton.
Embodiment 3
Described feed addictive is preferably made up of the raw material of following parts by weight: Tianmen Green bean noodle 8 parts; 3 parts, cattail pollen powder; 2 parts, ganoderma lucidum fruitbody powder; Bacillus subtilis microbial agent 6 parts; Angelica powder 2 parts; Aspergillus niger culture 4 parts; Licorice powder 1 part.
According to raw material composition, Tianmen Green bean noodle, cattail pollen powder, ganoderma lucidum fruitbody powder, uninteresting gemma bacillus agent, angelica powder, aspergillus niger culture, licorice powder are mixed and granulate.
The addition of this product in live pig daily ration is 600 grams/ton.
Result of use is tested
The result of use test of the application embodiment of the present invention 2 feed addictive in weanling pig:
Experimental animal selection plant average weight is the healthy sucking pig 120 of (11.5 ± 0.45) kg, and statistical analysis weight differences is not remarkable.Adopt single-factor Randomized Designs, by 120 healthy sucking pigs, by male and female half and half, be divided into 2 groups (control group and test group), often organize 6 repetitions, each repetition 10 pigs.Test group adds the obtained product of the present invention 800 grams/ton of embodiment 2, and control group adds other products similar.Feed continuously 25 days, compared with control group, use invention feed addictive can obtain following effect:
| Project | Test group | Control group | Remarks |
| Starting weight (kg) | 11.08 | 11.12 | |
| End heavy (kg) | 23.57 | 22.39 | |
| Daily ingestion amount (g) | 750.5 | 750.2 | |
| Daily gain (g) | 499.6 | 450.8 | |
| Feedstuff-meat ratio | 1.50 | 1.66 | |
| Diarrhea rate (%) | 4.1 | 8.0％ |
Result of the test shows: sucking pig is when daily ingestion amount is identical, the edible feed adding product of the present invention, effectively can improve the daily gain of sucking pig, reduce feedstuff-meat ratio, greatly reduce the diarrhea rate of sucking pig simultaneously, also demonstrate the utilization rate in animal body that feed addictive of the present invention effectively can improve feed, improve the immunity of sucking pig simultaneously; Carry out sensory evaluation scores to the hair color of experiment pig, experimental group scoring reaches 8.9 points, and control group is 6.7 points.
Claims (7)
1. a feed addictive, is made up of the raw material of following parts by weight: Tianmen Green bean noodle 5-8 part; Cattail pollen powder 3-6 part; Ganoderma lucidum fruitbody powder 1-3 part; Bacillus subtilis microbial agent 2-6 part; Angelica powder 2-6 part; Aspergillus niger culture 2-4 part; Licorice powder 1-2 part; Described aspergillus niger preserving number is CGMCC NO.7927.
2. feed addictive according to claim 1, is characterized in that, the preparation method of aspergillus niger culture is as follows:
After slant strains activation culture is transferred to and slant medium cultivates acquisition seed step by step, seed is added in the fermentation vat that sterilising medium is housed or pallet and mixes rear cultivation, bent material cultivation temperature controls at 18-26 DEG C, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat compost cover with mycelia can terminate cultivate, culture medium in advance through thermophilic digestion sterilization treatment, sterilising conditions control temperature 121 DEG C, 1 hour time;
Drying and crushing: fermentation ends compost carries out drying on fluid bed or other drying equipments, baking temperature controls, at 60 DEG C, to be dried to moisture below 10%, then to be pulverized by solid culture medium, and crushing material particle diameter is more than 60 orders.
3. feed addictive according to claim 1, is characterized in that, the raw material of parts by weight consists of: Tianmen Green bean noodle 6 parts; 5 parts, cattail pollen powder; 2 parts, ganoderma lucidum fruitbody powder; Bacillus subtilis microbial agent 4 parts; Angelica powder 4 parts; Aspergillus niger culture 3 parts; Licorice powder 2 parts.
4. feed addictive according to claim 1, is characterized in that, the raw material of parts by weight consists of: Tianmen Green bean noodle 8 parts; 3 parts, cattail pollen powder; 2 parts, ganoderma lucidum fruitbody powder; Bacillus subtilis microbial agent 6 parts; Angelica powder 2 parts; Aspergillus niger culture 4 parts; Licorice powder 1 part.
5. prepare a method for the feed addictive as described in claim 1-4, comprise the steps:, according to raw material composition, Tianmen Green bean noodle, cattail pollen powder, ganoderma lucidum fruitbody powder, uninteresting gemma bacillus agent, angelica powder, aspergillus niger culture, licorice powder are mixed granulation.
6. the preparation method of a kind of feed addictive according to claim 5, is characterized in that, respectively lucid asparagus, cattail pollen, glossy ganoderma, Radix Angelicae Sinensis, Radix Glycyrrhizae being crushed to particle diameter through pulverizer is less than 1 millimeter.
7. the preparation method of a kind of feed addictive according to claim 5, is characterized in that, the preparation method of described aspergillus niger culture is as follows:
After slant strains activation culture is transferred to and slant medium cultivates acquisition seed step by step, seed is added in the fermentation vat that sterilising medium is housed or pallet and mixes rear cultivation, bent material cultivation temperature controls at 18-26 DEG C, humidity 80-85%, every 6-10 hour stirring once, incubation time 5-8 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat compost cover with mycelia can terminate cultivate, culture medium in advance through thermophilic digestion sterilization treatment, sterilising conditions control temperature 121 DEG C, 1 hour time;
Drying and crushing: fermentation ends compost carries out drying on fluid bed or other drying equipments, baking temperature controls, at 60 DEG C, to be dried to moisture below 10%, then to be pulverized by solid culture medium, and crushing material particle diameter is more than 60 orders.
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Application publication date: 20150916 |