CN104381596A - Chicken feed additive product - Google Patents
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- CN104381596A CN104381596A CN201410510457.7A CN201410510457A CN104381596A CN 104381596 A CN104381596 A CN 104381596A CN 201410510457 A CN201410510457 A CN 201410510457A CN 104381596 A CN104381596 A CN 104381596A
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Abstract
The invention provides a chicken feed additive product, and relates to the field of feeds. The invention especially relates to a feed additive which is composed of the following components, by weight: 1-2 parts of stachyose, 1-3 parts of ginger, 1-4 parts of a glossy privet fruit extract, 10-15 parts of bacillus subtilis powder, 15-25 parts of lactobacillus plantarum powder, and 5-10 parts of lucid ganoderma fungus solid fermentation culture. A preparation method of the feed additive product comprises the following steps: the above raw materials are crushed and mixed; and the mixture is granulated by using a granulator. With the product provided by the invention, dose of antibiotic drugs in animal raising can be effectively reduced, animal meat product safety can be improved, animal feed utilization efficiency can be improved, animal immunity can be improved, and feeding benefit can be improved.
Description
Technical field:
The invention belongs to field of fodder, particularly relate to feed addictive.
Background technology:
Be derived from more natural natural materials and not only there is good nutritive peculiarity, also there is the effects such as antiviral, anti-inflammatory, anti-oxidant, conditioner body immunity function simultaneously, have broad application prospects.
The main component of ginger is flavone compound, phenols, lactone, Coumarins, aldehyde ketone, the trace element such as a small amount of iron, calcium, silicon, manganese, lead, nickel, aluminium, copper, zinc and vitamin B complex, vitamin A, and several amino acids, enzyme, polysaccharide and abundant arsanilic acid etc." natural antibiotics " that ginger or both at home and abroad expert generally acknowledge, because it not only has the effect of the external invader of defence and infection pathogen diffusion, also has the effects such as antiviral, anti-inflammatory, anti-oxidant, conditioner body immunity function.
The fruit of glossy privet is also known as glossy privet reality, purpleflower holly fruit, Chinese wax tree, mouse Chinese catalpa.Exocarp is thin, and mesocarp is softer, easily peels off, and endocarp is wooden, yellowish-brown, and tool indulges rib, breaks rear seed and is generally 1, kidney shape, atropurpureus, oiliness.Odorless, sweet, the micro-bitterness of taste.Fruit of glossy privet fruit is containing oleanolic acid (Oleanolic acid), sweet mellow wine, glucose, palmitic acid, stearic acid, oleic acid and leukotrienes.Pericarp is containing ursolic acid (Ursolic acid), oleanolic acid, acetyloleanolic acid (Acetyloleanolic acid).Seed fatty oily 14.9%, in oil, palmitic acid and stearic acid are 19.5%, oleic acid and leukotrienes etc. is 80.5%.The still trace element such as cupric, zinc, iron, manganese in the fruit of glossy privet.
Fruit of glossy privet tonifying liver kidney yin, crow must improving eyesight, and modern medicine study thinks that the fruit of glossy privet can suppress the effect of helicobacter pylori to treat stomach trouble, also has the treatment suppressing purine abnormal metabolism for gout and hyperuricemia.For the deficiency of liver-yin and kidney-yin, soreness of waist tinnitus, poliosis; Unseeing, poor vision; Fever due to yin deficiency, stomach trouble and gout and and hyperuricemia.The fruit of glossy privet is clinical conventional Chinese herbal medicine simply, has begun one's study it in recent years as the effect of feed addictive in Production of Livestock and Poultry.
Glossy ganoderma [Ganoderema lucidum (Leyss ex Fr.) karst], also known as polyporus lucidus, belongs to Basidiomycotina, Aphyllophorales, Ganodermataceae, Ganoderma.Doctor's allusion quotation in successive dynasties playbacks glossy ganoderma in " medicine-feeding ", and its ranking is before ginseng.Shennong's Herbal, Compendium of Material Medica are recorded, glossy ganoderma materials for improving vision, tonifying liver gas, calm the nerves, beneficial essence, the beneficial motive, beneficial temper, beneficial lung qi, kidney-nourishing gas, logical nine orifices, ear acute hearing, bright, the sharp joint of order, diuresis, skin maintenance, be first-class excellent tonic product.GL-B can promote the synthesis of protein, nucleic acid, has facilitation to the renewal of serum, liver and bone marrow cell protein or nucleic acid, synthesis, and it is one of glossy ganoderma principle active component of strengthening the body resistance to consolidate the constitution.GL-B is mainly present in lucid ganoderma fungus fructification, sclerotium, mycelium or zymotic fluid; The ganoderic acid be present in glossy ganoderma has pain relieving, calmness, suppression histamine releasing, detoxifies, protects the liver, malicious tumoricidal function, is one of principle active component of glossy ganoderma.
A kind of composite feed additive product, application number is 201210182772.2, which disclose a kind of composite feed additive product, composed as follows: FOS 10-15%, soya-bean polypeptides 10-20%, fruit of glossy privet extract 10-25%, bacillus subtilis freeze-dried vaccine powder, Ganoderma Lucidum solid fermentation culture; Bacillus subtilis (Bacillus subtilis) is CGMCC 6012, and aforementioned proportion is mass percent; Product of the present invention effectively can reduce the use amount of antibiotics in letting animals feed, improves the security of animal meat product, improves the food utilization efficiency of animal, strengthens the immunity of animal, improves raise benefit.
It is significant to feed additive industry development how effective exploitation obtains green feed additive product with natural plants or fungi.
Summary of the invention:
The invention provides a kind of additive agent for chicken feed product; Parts by weight are composed as follows:
Stachyose 1-2, ginger 1-3, glossy privet fruit extract 1-4, bacillus subtilis bacterium powder 10-15, Lactobacillus plantarum bacterium powder 15-25, Ganoderma Lucidum solid fermentation culture 5-10.
Described feedstuff additive product preparation method is: be made as particle by comminutor after being pulverized and mixed by above-mentioned each raw material.
Bacillus subtilis (Bacillus subtilis) is CGMCC6012.Lactobacillus plantarum is CGMCC No.9405.
In the present invention, Lactobacillus plantarum CGMCC No.9405 bacterial strain feature is as follows: examine under a microscope, this bacterial strain is rod-short, and Gram's staining is positive, and atrichia does not produce gemma; On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.
Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indoles experiment (+), motility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), produce hydrogen sulfide gas (-), in pH4.0MRS culture medium, grow (+).
Lactobacillus plantarum of the present invention adopts following flow process to carry out seed selection:
The original bacterial classification that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → nitrosoguanidine (NTG) mutagenesis → plasma mutagenesis → dull and stereotyped primary dcreening operation → shaking flask sieves → mitotic stability test again.
Starting strain of the present invention is in MRS dextrose culture-medium, and the throughput rate of its lactic acid is 1.5g/L/d, almost stops growing when medium pH is 3.5, is 0.34mg/h/kg Chinese cabbage to the decomposition rate of natrium nitrosum.Starting strain is the greenfeed that Li Zheng is collected in Fattening Sheep field, Yanchi county Ningxia, acquisition time on September 15th, 2013.
In order to improve the decomposition rate of its production of lactic acid speed, acid-fast ability and nitrite, DES and NTG technology is adopted to carry out mutagenesis to this bacterial classification successively, after mutagenesis, bacterial strain adopts MRS calcium carbonate flat board to carry out primary dcreening operation, then 500mL shake flask fermentation is adopted, biosensor analysis instrument carries out multiple sieve to Producing Strain, the lactobacillus plantarum strain that seed selection is excellent, then does passage assays, evaluates its genetic stability.
Lactobacillus plantarum tlj-2014 genetic stability result shows: through continuous passage ten times, property indices is all more stable, and heredity is better, and proterties is not replied, therefore using the object bacterial strain that Lactobacillus plantarum tlj-2014 obtains as seed selection.
Empirical tests finds: the production of lactic acid speed of this mutagenic strain can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L; Can survive under pH is the condition of 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate.
Therefore adopt this bacterial classification produce pickles, whole sweat nitrite concentration at below 5mg/kg, far below the content specified in standard GB/T 2714-2003 (20mg/kg).
Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 2nd, 2014 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101), preserving number is CGMCC NO.9405.
1.DES mutagenic and breeding
1) on super-clean bench, get Lactobacillus plantarum L mono-ring on test tube slant, access is equipped with in the 250mL triangular flask of 50mL culture medium MRS (without agar, glucose 20g/L) culture medium, 200rpm, cultivate about 12h for 37 DEG C, make thalline be in logarithmic growth in earlier stage.
2) get 5mL bacterium liquid, the centrifugal 10min of 5000rpm collects thalline, with brine 2 times.
3) 10 are diluted to pH7.0 phosphate buffer
7individual/mL bacteria suspension.
4) get the kaliumphosphate buffer of 32mL pH7.0,8mL bacteria suspension, 150mL triangular flask that 0.4mL DES to put into rotor in advance fully mix, make DES ultimate density be 1% (v/v).
5) in 37 DEG C of shaking tables, 150rpm reacts 30min, gets 1mL mixed liquor, adds 0.5mL 25%Na
2s
2o
3solution stopped reaction.
6) suitably dilute, get last dilution bacterium liquid 0.2mL, coat in calcium carbonate screening and culturing base (the calcium carbonate MRS culture medium containing 100g/L glucose) plate.Cultivate after 2 ~ 3 days at 37 DEG C, adopting photolithography the bacterial strain of this screening flat board to be transferred to pH is on the upper and natrium nitrosum screening and culturing base (single nitrogenous source is the modification MRS screening and culturing base of 2g/L natrium nitrosum) of LPHMRS culture medium (low ph value modification MRS culture medium) of 1.5,1.8 and 2.0.
7) cultivate after 2 ~ 3 days at 37 DEG C, choosing colony is comparatively large, can grow respectively and on LPHMRS culture medium, natrium nitrosum screening and culturing base on calcium carbonate screening and culturing base.Through Preliminary screening, the bacterium colony called after Lactobacillus plantarum L1 that picking goes out.
2. nitrosoguanidine mutagenesis
1) on super-clean bench, get Lactobacillus plantarum L1 mono-ring on test tube slant, access is equipped with in the 250mL triangular flask of 50mL culture medium MRS (without agar) culture medium (concentration of glucose is 60g/L), 200rpm, cultivate about 12h for 37 DEG C, make thalline be in logarithmic growth in earlier stage.
2) get the centrifugal 10min of 5mL bacterium liquid 5000rpm and collect thalline, with brine 2 times.
3) 10 are diluted to pH6.0 phosphate buffer
7individual/mL bacteria suspension.
4) get 10mL bacteria suspension to be transferred in 100mL triangular flask, add the NTG of 10mg, be mixed with the NTG solution that final concentration is 10mg/mL, and add 4-5 and drip acetone, be beneficial to NTG and dissolve.
5) at 37 DEG C, the centrifugal 10min of 200rpm oscillating reactions 30min, 5000rpm collects thalline, with SPSS washing several, and stopped reaction.
6) suitably dilute, get last dilution bacterium liquid 0.2mL, coat in calcium carbonate screening and culturing base (the calcium carbonate MRS culture medium containing 100g/L glucose) plate.Cultivate after 2 ~ 3 days at 37 DEG C, adopting photolithography the bacterial strain of this screening flat board to be transferred to pH is on the upper and natrium nitrosum screening and culturing base (single nitrogenous source is the modification MRS screening and culturing base of 2g/L natrium nitrosum) of LPHMRS culture medium (low ph value modification MRS culture medium) of 1.5,1.8 and 2.0.
7) select bacterial strain method: choosing colony is comparatively large, to grow on LPHMRS culture medium, natrium nitrosum screening and culturing base respectively and on calcium carbonate screening and culturing base.Through Preliminary screening, picking 100 meets the bacterium colony of above condition.
3. shaking flask is sieved again
1) on super-clean bench, get Lactobacillus plantarum one ring on each test tube slant respectively, access is equipped with in the 250mL triangular flask of 50mL culture medium MRS (without agar) culture medium (concentration of glucose is 100g/L), 200rpm, cultivate about 15h, make thalline be in mid log phase for 37 DEG C.
2) get 5mL bacterium liquid respectively, LPHMRS fluid nutrient medium (low ph value modification MRS culture medium) and the natrium nitrosum liquid screening medium (single nitrogenous source is the modification MRS screening and culturing base of 2g/L natrium nitrosum) upper (note: adopt 250mL triangular flask) that 50mL calcium carbonate screens in fluid nutrient medium (the calcium carbonate MRS culture medium containing 250g/L glucose) plate, pH is 1.5,1.8 and 2.0 is equipped with in access.200rpm, cultivates 3-4 days for 37 DEG C, detects the wear rate that Pfansteihl in calcium carbonate screening fluid nutrient medium produces speed, biomass in LPHMRS fluid nutrient medium and natrium nitrosum liquid screening medium nitrite every day respectively.After fermentation ends, compare the wear rate that Pfansteihl in the calcium carbonate screening fluid nutrient medium of 100 strain bacterial classifications produces speed, biomass in LPHMRS fluid nutrient medium and natrium nitrosum liquid screening medium nitrite.
3) bacterial strain that high Pfansteihl produces speed, the wear rate of tolerate low pH (this bacterial classification only can grow in the minimum culture medium for pH1.8) and nitrite is high is selected to have concurrently, by its called after L2 bacterium.
4. genetic stability test
L2 bacterium is gone down to posterity for continuous ten times on inclined-plane, and detects the fermentation situation after at every turn going down to posterity by the method that shaking flask is sieved again.Experiment finds, inclined-plane goes down to posterity for continuous ten times, and this bacterial classification proterties does not have significant change, and property indices is all normal, illustrates that the genetic stability of this bacterial classification is stronger.Strain Designation is Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014.
5.5L fermentation tank is tested
1) Lactobacillus plantarum L2 mono-ring on inclined-plane is got, access is equipped with in the 250mL triangular flask of 50mL culture medium MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm, cultivates about 12h, makes thalline be in mid log phase for 37 DEG C.
2) access of the bacterial classification of logarithmic phase is equipped with in the 5L fermentation tank of 3L MRS fluid nutrient medium (initial glucose is 150g/L).Inoculum concentration is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), later stage Anaerobic culturel 63 hours.After fermentation ends, the lactic acid production of Lactobacillus plantarum L2 reaches 95g/L.Such lactic acid producing speed is beneficial to the Rapid Fermentation of pickles.
3) 3L pH being equipped with in the access of the bacterial classification of logarithmic phase is in the 5L fermentation tank of LPHMRS fluid nutrient medium (initial glucose is 50g/L) of 1.8.Inoculum concentration is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), and later stage anaerobism, zymotic fluid pH controls 1.8 by the NaOH of whole process 0.5mol/L, and total incubation time is 48 hours.After fermentation ends, the biomass detecting Lactobacillus plantarum L2 is 2.5g/L, illustrates that Lactobacillus plantarum L2 can survive in the environment of pH1.8.
4) access of the bacterial classification of logarithmic phase is equipped with in the 5L fermentation tank of 3L natrium nitrosum liquid screening medium (single nitrogenous source is the modification MRS screening and culturing base of 2g/L natrium nitrosum).Inoculum concentration is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), and later stage anaerobism, sweat adds the sodium nitrite solution of 20g/L according to the wear rate stream of nitrite, cultivates 2-3 days.After fermentation ends, calculate sweat Lactobacillus plantarum L2 to the degradation rate of natrium nitrosum.Found that: under this condition, L2 can reach 563mg/h/L to the degradation rate of natrium nitrosum.
5) the bacterial classification 10mL of logarithmic phase access be equipped with in the pretreated Chinese cabbage of 2kg, traditionally pickles method is processed, and within every 12 hours, measures the content of nitrite in pickles.Found that, in whole sweat, L2 bacterium is 9.8mg/h/kg Chinese cabbage to the decomposition rate of natrium nitrosum.Content of sodium nitrite in pickles all the time lower than 5mg/kg, far below the content specified in standard GB/T 2714-2003 (20mg/kg).
Preparation method is as follows for Ganoderma Lucidum solid fermentation culture:
Slant strains activation culture: be transferred on slant medium by the glossy ganoderma slant strains of preservation, cultivates 96-120 hours, covers with inclined-plane to mycelia for 22-28 DEG C; Optimum culturing temperature 25 DEG C.
1. liquid first order seed is cultivated: the one piece of access of above-mentioned glossy ganoderma slant strains picking be equipped with in 500 ml shake flasks of 100 milliliters of culture mediums and carry out first order seed cultivation, condition of culture: cultivate 96-130 hours for rotary shaker 80-180 rev/min, 22-28 DEG C; Rotary shaker 150 revs/min is advisable, and cultivation temperature is advisable with 25 DEG C.
2. liquid two stage seed culture: one-level shake-flask seed is equipped with in 500 ml shake flasks of 100 milliliters of culture mediums with the access of 5-20% inoculum concentration and carries out secondary seed cultivation, condition of culture: cultivate 96-130 hours for rotary shaker 80-180 rev/min, 22-28 DEG C; Suitable inoculum concentration is 10%, and rotary shaker 150 revs/min is advisable, and cultivation temperature is advisable with 25 DEG C.
3. solid fermentation is cultivated: be equipped with in the 500L solid-state fermentation tank of solid fermentation culture medium after sterilizing by second-level shake flask seed with the access of 5-10% inoculum concentration, condition of culture: charge 50%, cultivation temperature 20-27 DEG C, humidity 75-90%, ventilation 0.3-1.0 (V/V), incubation time 15-40 days.Stirring 10 minutes is opened every 3-10 days.Adopt SGF solid-state fermentation tank.
4. fermented and cultured terminates, and selects the multiple drying means such as fluid bed, by dry materials to moisture below 7%.Solid fermentation material is pulverized.
The carbon source of solid fermentation culture medium of the present invention mainly selects the conventional raw materials such as wheat bran, glucose, sucrose, starch, corn flour, corncob (after pulverizing), and nitrogenous source can select groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, dusty yeast etc.; The inorganic salts added are needed to have potassium dihydrogen phosphate, magnesium sulfate.
Described glossy privet fruit extract preparation method is as follows:
The fruit of glossy privet is pulverized, and after crossing 40-50 mesh sieve, add fruit of glossy privet 3-6 times of weight absolute ethyl alcohol Soakage extraction, control temperature is 60-65 DEG C of 3-4 hour, and extract is concentrated, drying obtains ethanol extract; Add 75-85 DEG C of hot water in fruit of glossy privet residue after alcohol extract, hot water addition is 2-4 times of fruit of glossy privet residue weight, and processing time 30-50 minute, extracts 2-3 time continuously, by spraying dry after extract Vacuum Concentration, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, crosses 60 mesh sieves, obtain glossy privet fruit extract.
Described bacillus subtilis is bacillus subtilis CGMCC6012, and in freeze-dried vaccine powder, number of viable is 4-8 × 10
8individual/g.Described composite feed additive is obtained by following methods:
After pulverizing, Ganoderma Lucidum culture, FOS, ginger, glossy privet fruit extract, Lactobacillus plantarum and bacillus subtilis freeze-dried vaccine powder are proportionally pulverized and mixed granulation and obtain feed addictive.
The invention provides a kind of Feed Manufacturing bacillus subtilis (Bacillus subtilis) bacterial classification, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, preservation address is positioned at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, (postcode 100101), deposit number is CGMCC No.6012.
Beneficial effect:
Bacillus subtilis: there is following function: 1. oxygen consumption produces acid, and regulating intestinal canal microecological balance, reaches the diseases such as pre-anti-diarrhea, diarrhea; 2. secrete multiple enzyme, improve feed digestion utilization rate; 3. produce various nutrients, participate in body metabolism; 4. improve animal immunizing power.The fruit of glossy privet of the present invention carries out pulverize and break cellular wall process, function factor in the fruit of glossy privet is made to realize full price stripping and high efficiency extraction utilization, the effective extraction, particularly control temperature stage extraction that adopt organic solvent and hot water to realize wherein different efficacies composition respectively effectively improve extraction efficiency and the quality of glossy privet fruit extract especially.Stachyose obviously can improve microbial population ratio in enteron aisle, and it is the activation and proliferation factor of Bifidobacterium in intestines, can reduce and suppress the generation of corrupt substance in intestines, suppresses the growth of harmful bacteria, regulating intestinal canal inner equilibrium; The present invention is composite by science, and bacillus subtilis, Lactobacillus plantarum, Ganoderma Lucidum, ginger are achieved organic assembling, and the interpolation of highly-acidproof Lactobacillus plantarum effectively can pass through stomach, and the effect improving Lactobacillus plantarum plays.Product of the present invention effectively can reduce the use amount of antibiotics in letting animals feed, improves the security of animal meat product, improves the food utilization efficiency of animal, strengthens the immunity of animal, improves raise benefit.
Specific implementation method:
In the present invention, Ganoderma Lucidum culture production process is as follows:
(1) slant strains activation culture:
(2) liquid first order seed is cultivated:
(3) liquid two stage seed culture:
(4) solid fermentation is cultivated:
(5) fermented and cultured terminates, and selects the multiple drying means such as fluid bed, by dry materials to moisture below 7%.Solid fermentation material is pulverized.
The present invention's lucidum strain used is purchased from Chinese industrial Culture Collection, bacterium CICC14080.
In the present invention, slant medium composition can be PDA slant medium or other suitable culture mediums.
The carbon source of solid fermentation culture medium of the present invention mainly selects the conventional raw materials such as wheat bran, glucose, sucrose, starch, corn flour, corncob (after pulverizing), and nitrogenous source can select groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, dusty yeast etc.; The inorganic salts added are needed to have potassium dihydrogen phosphate, magnesium sulfate.
Liquid fermentation fermentation medium composition in the present invention: starch 2-3%, sucrose 3-5%, ginger 1%, glucose 1-3%, peptone 0.5%, De-fatted soya protein Powder 2-3%, potassium dihydrogen phosphate 0.13-0.2%, magnesium sulfate 0.1-0.15%, Cobastab
12PPm, PH6-7.
In the present invention, glossy ganoderma cultural method is applicable to belonging to other bacterial classification together and carries out large scale fermentation cultivation by fermentation method.
Induction mutation of bacterium
Take ultraviolet mutagenesis method: be starting strain with CICC10023, make bacteria suspension, coat on culture medium plate, cultivate in 37 DEG C of incubators after ultraviolet irradiation 90s.
Bacterial screening
Choose 30 single bacterium colonies of looking strong from 90s plate, be inoculated on inclined-plane, in 37 DEG C of incubators, cultivate 24 hours, 0--4 DEG C stored refrigerated; With original strain in contrast, adopt conventional herd breeding condition, sodium carboxymethylcellulose is as carbon source and proteolysis circle method screening cellulase-producing and the strong bacterial strain of protease, screening obtains cellulase-producing and strong Strains B. subtilis (Bacillus subtilis) L1 of protease, this bacterial strain cellulase-producing energy force rate original strain improves 25%, produces protease energy force rate original strain and improves 52%.By this bacterial classification in the center preservation of China General Microbiological culture presevation administration committee's common micro-organisms, deposit number is CGMCC No 6012.
This product bacillus subtilis used (Bacillus subtilis) bacterial classification, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, preservation address is positioned at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, (postcode 100101), deposit number is CGMCC No6012.
Research shows by experiment: product of the present invention have good suppression Escherichia coli, staphylococcus aureus, etc. the characteristic of pathogenic bacteria.Adopt the fungistatic effect of cylinder plate method to ganoderma lucidum feedstuff additive product to be studied, test adopts 10 times of water extracts of feed addictive to study.Experimental result is in table 1, and result shows that product of the present invention has the superperformance suppressing pathogenic bacteria growth.
The fungistatic effect of table 1 feedstuff additive product
| Pathogenic bacteria | Staphylococcus aureus | Escherichia coli |
| Antibacterial circle diameter (mm) | 23 | 28 |
The stable performance of product of the present invention, use safety, with other feed addictive all without incompatibility.The addition of product of the present invention in feed is 1-2%, and it completely can substitute antibiotics medicine, and noresidue, free from environmental pollution.After feeding, significantly can strengthen immunity and the premunition of animal body, growth promoting effects, improve daily gain and feed conversion rate, and have resisting stress, antioxidant effect; There is stronger prevention effect to intestinal bacteriosis simultaneously.According to test, the aquatic products of feeding feed addictive of the present invention, quality improves, and meet the greenization production requirement of animal food, Social benefit and economic benefit is very remarkable.
The Chinese tradition edible mushroom that product of the present invention is produced with biological fermentation process is for raw material, constant product quality is controlled, product noresidue in vivo, non-environmental-pollution, without the resistance to the action of a drug, and immunity can be improved, there is strong bacteriostasis, can culture benefit be improved.Product meets the requirement of green feed additive completely.
Embodiment 1
A kind of additive agent for chicken feed product; Parts by weight are composed as follows:
Stachyose 1, ginger 2, glossy privet fruit extract 3, bacillus subtilis bacterium powder 10-152, Lactobacillus plantarum bacterium powder 20, Ganoderma Lucidum solid fermentation culture 8.
Described feedstuff additive product preparation method is: be made as particle by comminutor after being pulverized and mixed by above-mentioned each raw material.
Embodiment 2 one kinds of additive agent for chicken feed products; Parts by weight are composed as follows:
Stachyose 1, ginger 3, glossy privet fruit extract 1, bacillus subtilis bacterium powder 15, Lactobacillus plantarum bacterium powder 15, Ganoderma Lucidum solid fermentation culture 5.
Embodiment 3 one kinds of additive agent for chicken feed products; Parts by weight are composed as follows:
Stachyose 1, ginger 1 glossy privet fruit extract 4, bacillus subtilis bacterium powder 15, Lactobacillus plantarum bacterium powder 25, Ganoderma Lucidum solid fermentation culture 10.
Feeding effect is tested:
Test and carry out at Yanchi County, ningxia chicken house-22 days in September, 2013-December 29.Control group is market like product, and test group is product of the present invention of feeding, and addition is for being 3%.
Test chicken divides into groups: test adopts single factor experiment design, and experiment material selects 800 blue brown 126 age in days laying hens in sea, and be divided into two groups, often group establishes 6 repetitions;
Fed control group and feed group of the present invention respectively.
Feeding method: free choice feeding;
Testing index comprises: body weight---start and at the end of twice, feed intake, survival rate.Laying rate, egg size, feedstuff-egg ratio, Egg Quality and quality of egg etc., the results are shown in Table 1, table 2.
Table 1 the present invention is on the impact of laying hen expected date of childbirth production performance
Table 2 the present invention is on laying hen 2% laying rate---28 week age egg laying performance impact
As can be seen from table 1 and table 2 result of the test we, feed of the present invention has good behaviour in egg size, laying rate, feedstuff-egg ratio, feed intake and survival rate etc., has absolutely proved science and the advance of product of the present invention.
Claims (5)
1. an additive agent for chicken feed product; Parts by weight are composed as follows: stachyose 1-2, ginger 1-3, glossy privet fruit extract 1-4, bacillus subtilis bacterium powder 10-15, Lactobacillus plantarum bacterium powder 15-25, Ganoderma Lucidum solid fermentation culture 5-10.
2. additive agent for chicken feed product according to claim 1, it is characterized in that: described bacillus subtilis is CGMCC6012, Lactobacillus plantarum is CGMCC No.9405.
3. additive agent for chicken feed product according to claim 1-2, it is characterized in that: described additive agent for chicken feed product weight number is composed as follows: stachyose 1, ginger 2, glossy privet fruit extract 3, bacillus subtilis bacterium powder 10-152, Lactobacillus plantarum bacterium powder 20, Ganoderma Lucidum solid fermentation culture 8.
4. additive agent for chicken feed product according to claim 1-3, is characterized in that: described additive agent for chicken feed product weight number is composed as follows:
Stachyose 1, ginger 3, glossy privet fruit extract 1, bacillus subtilis bacterium powder 15, Lactobacillus plantarum bacterium powder 15, Ganoderma Lucidum solid fermentation culture 5.
5. additive agent for chicken feed product according to claim 1-4, is characterized in that: described additive agent for chicken feed product weight number is composed as follows:
Stachyose 1, ginger 1 glossy privet fruit extract 4, bacillus subtilis bacterium powder 15, Lactobacillus plantarum bacterium powder 25, Ganoderma Lucidum solid fermentation culture 10.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028954A (en) * | 2015-08-26 | 2015-11-11 | 天津天绿健科技有限公司 | Feed additive product |
| CN105961828A (en) * | 2016-03-22 | 2016-09-28 | 安徽旺旺禽业科技有限公司 | Ganoderma lucidum feed for broiler chicken and preparation method thereof |
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| CN109275781A (en) * | 2018-11-19 | 2019-01-29 | 天水市畜牧技术推广站 | It is a kind of using paper mulberry as poultry anti-stress feed of raw material and preparation method thereof |
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| CN105028954A (en) * | 2015-08-26 | 2015-11-11 | 天津天绿健科技有限公司 | Feed additive product |
| CN105961828A (en) * | 2016-03-22 | 2016-09-28 | 安徽旺旺禽业科技有限公司 | Ganoderma lucidum feed for broiler chicken and preparation method thereof |
| CN106359901A (en) * | 2016-08-29 | 2017-02-01 | 青岛润达生物科技有限公司 | Compound product of glossy ganoderma polysaccharide and lactic acid bacteria |
| CN109275781A (en) * | 2018-11-19 | 2019-01-29 | 天水市畜牧技术推广站 | It is a kind of using paper mulberry as poultry anti-stress feed of raw material and preparation method thereof |
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