CN104509697A - Nutritional feed additive product - Google Patents
Nutritional feed additive product Download PDFInfo
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- CN104509697A CN104509697A CN201410495005.6A CN201410495005A CN104509697A CN 104509697 A CN104509697 A CN 104509697A CN 201410495005 A CN201410495005 A CN 201410495005A CN 104509697 A CN104509697 A CN 104509697A
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Abstract
The invention discloses a nutritional feed additive product, belongs to the feed field, and particularly relates to the feed additive. The invention provides the nutritional feed additive product comprising the following components in parts by weight: 1-3 parts of stachyose, 3-10 parts of soybean polypeptide, 3-5 parts of an angelica sinensis extract, 2-6 parts of lentinan, 16-23 parts of a bacillus subtilis powder, 15-25 parts of a lactobacillus plantarum powder, 2-5 parts of a raw ginger powder, and 3-8 parts of a ganoderma lucidum solid fermentation culture. Through scientific compounding, bacillus subtilis, lactobacillus plantarum and ganoderma lucidum are in organic combination, and the content of probiotic components in the additive product is strengthened; especially, the traditional Chinese medicine ingredients are added in culturing of ganoderma lucidum; the product can effectively reduce the usage amount of antibiotic drugs in feeding animals, improves the safety of animal meat products, improves animal feed utilization efficiency, enhances animal immunity, and improves the feeding efficiency.
Description
Technical field:
The invention belongs to field of fodder, particularly relate to feed addictive.
Background technology:
In modern livestock and poultry cultivation process, in order to prevention and therapy Animal diseases, the excessive antibiotic product of normal use guarantees animal health, but excessive antibiotic use often causes the quality of animal meat product and product thereof to decline and antibiotic resistance strengthens, and causes human health to be also subject to having a strong impact on of antibiotic problem by the transmission of food chain.
Be derived from more natural natural materials and not only there is good nutritive peculiarity, also there is the effects such as antiviral, anti-inflammatory, anti-oxidant, conditioner body immunity function simultaneously, have broad application prospects.
Soya-bean polypeptides absorbability easy to digest, promotes energetic supersession, thus shows anti-obesic action; Short overtiredly to refresh, the effect of muscle power; Increase the endurance of muscle, promote that myoglobins recovers; There are the effects such as low antigen, hypoallergenic;
Glossy ganoderma [Ganoderema lucidum (Leyss ex Fr.) karst], also known as polyporus lucidus, belongs to Basidiomycotina, Aphyllophorales, Ganodermataceae, Ganoderma.Doctor's allusion quotation in successive dynasties playbacks glossy ganoderma in " medicine-feeding ", and its ranking is before ginseng.Shennong's Herbal, Compendium of Material Medica are recorded, glossy ganoderma materials for improving vision, tonifying liver gas, calm the nerves, beneficial essence, the beneficial motive, beneficial temper, beneficial lung qi, kidney-nourishing gas, logical nine orifices, ear acute hearing, bright, the sharp joint of order, diuresis, skin maintenance, be first-class excellent tonic product.GL-B can promote the synthesis of protein, nucleic acid, has facilitation to the renewal of serum, liver and bone marrow cell protein or nucleic acid, synthesis, and it is one of glossy ganoderma principle active component of strengthening the body resistance to consolidate the constitution.GL-B is mainly present in lucid ganoderma fungus fructification, sclerotium, mycelium or zymotic fluid; The ganoderic acid be present in glossy ganoderma has pain relieving, calmness, suppression histamine releasing, detoxifies, protects the liver, malicious tumoricidal function, is one of principle active component of glossy ganoderma.
The composite vegetables extractive feed addictive patent No. is 201210143338.3, and a kind of composite vegetables extractive feed addictive of disclosure of the invention, the invention belongs to field of fodder, particularly feed addictive.Feed addictive of the present invention consists of: Inokopolyose, extractive of perilla, yeast cell wall extract, bacillus subtilis freeze-dried vaccine powder, FOS; Bacillus subtilis is CGMCC6012, and aforementioned proportion is mass percent.Product of the present invention effectively can reduce the usage quantity of antibiotics in letting animals feed, improve the immunity of letting animals feed, improve the security of animal meat product, improve the food utilization efficiency of animal, reduce the use of antibiotic and medicine in conventional raising, strengthen the resistivity of animal, improve raise benefit.
It is significant to feed additive industry development how effective exploitation obtains green feed additive product with natural plants or fungi.
Summary of the invention:
The invention provides a kind of nutrient feed additive product;
Parts by weight are composed as follows:
Stachyose 1-3, soya-bean polypeptides 3-10, Chinese angelica root extract 3-5, lentinan 2-6, bacillus subtilis powder 16-23, Lactobacillus plantarum bacterium powder 15-25, ginger powder 2-5, Ganoderma Lucidum solid fermentation culture 3-8.
Lactobacillus plantarum is CGMCC 9405, and bacillus subtilis (Bacillus subtilis) is CGMCC6012.
Preparation method is as follows for Ganoderma Lucidum solid fermentation culture:
Slant strains activation culture: be transferred on slant medium by the glossy ganoderma slant strains of preservation, cultivates 96-120 hours, covers with inclined-plane to mycelia for 22-28 DEG C; Optimum culturing temperature 25 DEG C.
1. liquid first order seed is cultivated: the one piece of access of above-mentioned glossy ganoderma slant strains picking be equipped with in 500 ml shake flasks of 100 milliliters of culture mediums and carry out first order seed cultivation, condition of culture: cultivate 96-130 hours for rotary shaker 80-180 rev/min, 22-28 DEG C; Rotary shaker 150 revs/min is advisable, and cultivation temperature is advisable with 25 DEG C.
2. liquid two stage seed culture: one-level shake-flask seed is equipped with in 500 ml shake flasks of 100 milliliters of culture mediums with the access of 5-20% inoculum concentration and carries out secondary seed cultivation, condition of culture: cultivate 96-130 hours for rotary shaker 80-180 rev/min, 22-28 DEG C; Suitable inoculum concentration is 10%, and rotary shaker 150 revs/min is advisable, and cultivation temperature is advisable with 25 DEG C.
3. solid fermentation is cultivated: be equipped with in the 500L solid-state fermentation tank of solid fermentation culture medium after sterilizing by second-level shake flask seed with the access of 5-10% inoculum concentration, condition of culture: charge 50%, cultivation temperature 20-27 DEG C, humidity 75-90%, ventilation 0.3-1.0 (V/V), incubation time 15-40 days.Stirring 10 minutes is opened every 3-10 days.Adopt SGF solid-state fermentation tank.
4. fermented and cultured terminates, and selects the multiple drying means such as fluid bed, by dry materials to moisture below 7%.Solid fermentation material is pulverized.
The carbon source of solid fermentation culture medium of the present invention mainly selects the conventional raw materials such as wheat bran, glucose, sucrose, starch, corn flour, corncob (after pulverizing), and nitrogenous source can select groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, dusty yeast etc.; The inorganic salts added are needed to have potassium dihydrogen phosphate, magnesium sulfate.
In above-mentioned solid fermentation culture medium, be added with mass ratio is after 2-6% pulverizes, and crosses 40-50 object ginger.
Described Chinese angelica root extract preparation method is as follows:
Radix Angelicae Sinensis is pulverized, and after crossing 40-50 mesh sieve, add Radix Angelicae Sinensis 3-6 times of weight absolute ethyl alcohol Soakage extraction, adjusting temperature after control temperature 30-45 DEG C, 2-4 hour is 55-60 DEG C of 1-2 hour, and extract is concentrated, drying obtains ethanol extract; Add 75-85 DEG C of hot water in Radix Angelicae Sinensis residue after alcohol extract, hot water addition is 2-4 times of Radix Angelicae Sinensis residue weight, and processing time 30-50 minute, extracts 2-3 time continuously, by spraying dry after extract Vacuum Concentration, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, crosses 60 mesh sieves, obtain angelica extract.
Described bacillus subtilis is bacillus subtilis CGMCC6012, and in freeze-dried vaccine powder, number of viable is 4-8 × 10
8individual/g.Described composite feed additive is obtained by following methods:
Pulverize the components such as rear Ganoderma Lucidum culture, stachyose, soya-bean polypeptides, Chinese angelica root extract and bacillus subtilis freeze-dried vaccine powder and be proportionally mixed to get feed addictive.
Soya-bean polypeptides adopts commercially available soya-bean polypeptides product, and molecular weight is between 500Da ~ 700Da.
The invention provides a kind of Feed Manufacturing bacillus subtilis (Bacillus subtilis) bacterial classification, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, preservation address is positioned at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, (postcode 100101), deposit number is CGMCC No.6012.
In the present invention, Lactobacillus plantarum CGMCC 9405 bacterial strain feature is as follows: examine under a microscope, this bacterial strain is rod-short, and Gram's staining is positive, and atrichia does not produce gemma; On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.
Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indoles experiment (+), motility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), produce hydrogen sulfide gas (-), in pH4.0MRS culture medium, grow (+).
Lactobacillus plantarum of the present invention adopts following flow process to carry out seed selection:
The original bacterial classification that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → nitrosoguanidine (NTG) mutagenesis → plasma mutagenesis → dull and stereotyped primary dcreening operation → shaking flask sieves → mitotic stability test again.
Starting strain of the present invention is in MRS dextrose culture-medium, and the throughput rate of its lactic acid is 1.5g/L/d, almost stops growing when medium pH is 3.5, is 0.34mg/h/kg Chinese cabbage to the decomposition rate of natrium nitrosum.Starting strain is the greenfeed that Li Zheng is collected in Fattening Sheep field, Yanchi county Ningxia, acquisition time on September 15th, 2013.
In order to improve the decomposition rate of its production of lactic acid speed, acid-fast ability and nitrite, DES and NTG technology is adopted to carry out mutagenesis to this bacterial classification successively, after mutagenesis, bacterial strain adopts MRS calcium carbonate flat board to carry out primary dcreening operation, then 500mL shake flask fermentation is adopted, biosensor analysis instrument carries out multiple sieve to Producing Strain, the lactobacillus plantarum strain that seed selection is excellent, then does passage assays, evaluates its genetic stability.
Lactobacillus plantarum tlj-2014 genetic stability result shows: through continuous passage ten times, property indices is all more stable, and heredity is better, and proterties is not replied, therefore using the object bacterial strain that Lactobacillus plantarum tlj-2014 obtains as seed selection.
Empirical tests finds: the production of lactic acid speed of this mutagenic strain can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L; Can survive under pH is the condition of 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate.
Therefore adopt this bacterial classification produce pickles, whole sweat nitrite concentration at below 5mg/kg, far below the content specified in standard GB/T 2714-2003 (20mg/kg).
Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 2nd, 2014 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101), preserving number is CGMCC NO.9405.
1.DES mutagenic and breeding
1) on super-clean bench, get Lactobacillus plantarum L mono-ring on test tube slant, access is equipped with in the 250mL triangular flask of 50mL culture medium MRS (without agar, glucose 20g/L) culture medium, 200rpm, cultivate about 12h for 37 DEG C, make thalline be in logarithmic growth in earlier stage.
2) get 5mL bacterium liquid, the centrifugal 10min of 5000rpm collects thalline, with brine 2 times.
3) 10 are diluted to pH7.0 phosphate buffer
7individual/mL bacteria suspension.
4) get the kaliumphosphate buffer of 32mL pH7.0,8mL bacteria suspension, 150mL triangular flask that 0.4mL DES to put into rotor in advance fully mix, make DES ultimate density be 1% (v/v).
5) in 37 DEG C of shaking tables, 150rpm reacts 30min, gets 1mL mixed liquor, adds 0.5mL 25%Na
2s
2o
3solution stopped reaction.
6) suitably dilute, get last dilution bacterium liquid 0.2mL, coat in calcium carbonate screening and culturing base (the calcium carbonate MRS culture medium containing 100g/L glucose) plate.Cultivate after 2 ~ 3 days at 37 DEG C, adopting photolithography the bacterial strain of this screening flat board to be transferred to pH is on the upper and natrium nitrosum screening and culturing base (single nitrogenous source is the modification MRS screening and culturing base of 2g/L natrium nitrosum) of LPHMRS culture medium (low ph value modification MRS culture medium) of 1.5,1.8 and 2.0.
7) cultivate after 2 ~ 3 days at 37 DEG C, choosing colony is comparatively large, can grow respectively and on LPHMRS culture medium, natrium nitrosum screening and culturing base on calcium carbonate screening and culturing base.Through Preliminary screening, the bacterium colony called after Lactobacillus plantarum L1 that picking goes out.
2. nitrosoguanidine mutagenesis
1) on super-clean bench, get Lactobacillus plantarum L1 mono-ring on test tube slant, access is equipped with in the 250mL triangular flask of 50mL culture medium MRS (without agar) culture medium (concentration of glucose is 60g/L), 200rpm, cultivate about 12h for 37 DEG C, make thalline be in logarithmic growth in earlier stage.
2) get the centrifugal 10min of 5mL bacterium liquid 5000rpm and collect thalline, with brine 2 times.
3) 10 are diluted to pH6.0 phosphate buffer
7individual/mL bacteria suspension.
4) get 10mL bacteria suspension to be transferred in 100mL triangular flask, add the NTG of 10mg, be mixed with the NTG solution that final concentration is 10mg/mL, and add 4-5 and drip acetone, be beneficial to NTG and dissolve.
5) at 37 DEG C, the centrifugal 10min of 200rpm oscillating reactions 30min, 5000rpm collects thalline, with SPSS washing several, and stopped reaction.
6) suitably dilute, get last dilution bacterium liquid 0.2mL, coat in calcium carbonate screening and culturing base (the calcium carbonate MRS culture medium containing 100g/L glucose) plate.Cultivate after 2 ~ 3 days at 37 DEG C, adopting photolithography the bacterial strain of this screening flat board to be transferred to pH is on the upper and natrium nitrosum screening and culturing base (single nitrogenous source is the modification MRS screening and culturing base of 2g/L natrium nitrosum) of LPHMRS culture medium (low ph value modification MRS culture medium) of 1.5,1.8 and 2.0.
7) select bacterial strain method: choosing colony is comparatively large, to grow on LPHMRS culture medium, natrium nitrosum screening and culturing base respectively and on calcium carbonate screening and culturing base.Through Preliminary screening, picking 100 meets the bacterium colony of above condition.
3. shaking flask is sieved again
1) on super-clean bench, get Lactobacillus plantarum one ring on each test tube slant respectively, access is equipped with in the 250mL triangular flask of 50mL culture medium MRS (without agar) culture medium (concentration of glucose is 100g/L), 200rpm, cultivate about 15h, make thalline be in mid log phase for 37 DEG C.
2) get 5mL bacterium liquid respectively, LPHMRS fluid nutrient medium (low ph value modification MRS culture medium) and the natrium nitrosum liquid screening medium (single nitrogenous source is the modification MRS screening and culturing base of 2g/L natrium nitrosum) upper (note: adopt 250mL triangular flask) that 50mL calcium carbonate screens in fluid nutrient medium (the calcium carbonate MRS culture medium containing 250g/L glucose) plate, pH is 1.5,1.8 and 2.0 is equipped with in access.200rpm, cultivates 3-4 days for 37 DEG C, detects the wear rate that Pfansteihl in calcium carbonate screening fluid nutrient medium produces speed, biomass in LPHMRS fluid nutrient medium and natrium nitrosum liquid screening medium nitrite every day respectively.After fermentation ends, compare the wear rate that Pfansteihl in the calcium carbonate screening fluid nutrient medium of 100 strain bacterial classifications produces speed, biomass in LPHMRS fluid nutrient medium and natrium nitrosum liquid screening medium nitrite.
3) bacterial strain that high Pfansteihl produces speed, the wear rate of tolerate low pH (this bacterial classification only can grow in the minimum culture medium for pH1.8) and nitrite is high is selected to have concurrently, by its called after L2 bacterium.
4. genetic stability test
L2 bacterium is gone down to posterity for continuous ten times on inclined-plane, and detects the fermentation situation after at every turn going down to posterity by the method that shaking flask is sieved again.Experiment finds, inclined-plane goes down to posterity for continuous ten times, and this bacterial classification proterties does not have significant change, and property indices is all normal, illustrates that the genetic stability of this bacterial classification is stronger.Strain Designation is Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014.
5.5L fermentation tank is tested
1) Lactobacillus plantarum L2 mono-ring on inclined-plane is got, access is equipped with in the 250mL triangular flask of 50mL culture medium MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm, cultivates about 12h, makes thalline be in mid log phase for 37 DEG C.
2) access of the bacterial classification of logarithmic phase is equipped with in the 5L fermentation tank of 3L MRS fluid nutrient medium (initial glucose is 150g/L).Inoculum concentration is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), later stage Anaerobic culturel 63 hours.After fermentation ends, the lactic acid production of Lactobacillus plantarum L2 reaches 95g/L.Such lactic acid producing speed is beneficial to the Rapid Fermentation of pickles.
3) 3L pH being equipped with in the access of the bacterial classification of logarithmic phase is in the 5L fermentation tank of LPHMRS fluid nutrient medium (initial glucose is 50g/L) of 1.8.Inoculum concentration is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), and later stage anaerobism, zymotic fluid pH controls 1.8 by the NaOH of whole process 0.5mol/L, and total incubation time is 48 hours.After fermentation ends, the biomass detecting Lactobacillus plantarum L2 is 2.5g/L, illustrates that Lactobacillus plantarum L2 can survive in the environment of pH1.8.
4) access of the bacterial classification of logarithmic phase is equipped with in the 5L fermentation tank of 3L natrium nitrosum liquid screening medium (single nitrogenous source is the modification MRS screening and culturing base of 2g/L natrium nitrosum).Inoculum concentration is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), and later stage anaerobism, sweat adds the sodium nitrite solution of 20g/L according to the wear rate stream of nitrite, cultivates 2-3 days.After fermentation ends, calculate sweat Lactobacillus plantarum L2 to the degradation rate of natrium nitrosum.Found that: under this condition, L2 can reach 563mg/h/L to the degradation rate of natrium nitrosum.
5) the bacterial classification 10mL of logarithmic phase access be equipped with in the pretreated Chinese cabbage of 2kg, traditionally pickles method is processed, and within every 12 hours, measures the content of nitrite in pickles.Found that, in whole sweat, L2 bacterium is 9.8mg/h/kg Chinese cabbage to the decomposition rate of natrium nitrosum.Content of sodium nitrite in pickles all the time lower than 5mg/kg, far below the content specified in standard GB/T 2714-2003 (20mg/kg).
Beneficial effect:
Bacillus subtilis: there is following function: 1. oxygen consumption produces acid, and regulating intestinal canal microecological balance, reaches the diseases such as pre-anti-diarrhea, diarrhea; 2. secrete multiple enzyme, improve feed digestion utilization rate; 3. produce various nutrients, participate in body metabolism; 4. improve animal immunizing power.Radix Angelicae Sinensis of the present invention carries out pulverize and break cellular wall process, function factor in Radix Angelicae Sinensis is made to realize full price stripping and high efficiency extraction utilization, the effective extraction, particularly control temperature stage extraction that adopt organic solvent and hot water to realize wherein different efficacies composition respectively effectively improve extraction efficiency and the quality of angelica extract especially.
Stachyose is except having the physicochemical properties of general utility functions compound sugar, the most noticeable physiological property is that it obviously can improve microbial population ratio in enteron aisle, it is the activation and proliferation factor of Bifidobacterium in intestines, can reduce and suppress the generation of corrupt substance in intestines, suppress the growth of harmful bacteria, regulating intestinal canal inner equilibrium; Absorption and the utilization of trace elements iron, calcium can be promoted, to prevent osteoporosis; Can hepatotoxin be reduced, anticancer organic acid can be generated in intestines, have significant preventing cancer function;
The present invention is composite by science, bacillus subtilis, Lactobacillus plantarum, Ganoderma Lucidum are achieved organic assembling, enhance probiotic composition content wherein, particularly Ganoderma Lucidum with the addition of traditional Chinese medicine ingredients in cultivating, product of the present invention effectively can reduce the use amount of antibiotics in letting animals feed, improves the security of animal meat product, improves the food utilization efficiency of animal, strengthen the immunity of animal, improve raise benefit.
Specific implementation method:
In the present invention, Ganoderma Lucidum culture production process is as follows:
(1) slant strains activation culture:
(2) liquid first order seed is cultivated:
(3) liquid two stage seed culture:
(4) solid fermentation is cultivated:
(5) fermented and cultured terminates, and selects the multiple drying means such as fluid bed, by dry materials to moisture below 7%.Solid fermentation material is pulverized.
The present invention's lucidum strain used is purchased from Chinese industrial Culture Collection, bacterium CICC14080.
In the present invention, slant medium composition can be PDA slant medium or other suitable culture mediums.
The carbon source of solid fermentation culture medium of the present invention mainly selects the conventional raw materials such as wheat bran, glucose, sucrose, starch, corn flour, corncob (after pulverizing), and nitrogenous source can select groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, dusty yeast etc.; The inorganic salts added are needed to have potassium dihydrogen phosphate, magnesium sulfate.
Also being added with mass ratio is in the fermentation medium cross 40-50 object ginger after 2-6% pulverizes;
Liquid fermentation seed culture medium composition in the present invention: starch 2-3%, sucrose 3-5%, glucose 1-3%, peptone 0.5%, De-fatted soya protein Powder 2-3%, potassium dihydrogen phosphate 0.13-0.2%, magnesium sulfate 0.1-0.15%, Cobastab
12PPm, PH6-7.
In the present invention, glossy ganoderma cultural method is applicable to belonging to other bacterial classification together and carries out large scale fermentation cultivation by fermentation method.
Induction mutation of bacterium
Take ultraviolet mutagenesis method: be starting strain with CICC10023, make bacteria suspension, coat on culture medium plate, cultivate in 37 DEG C of incubators after ultraviolet irradiation 90s.
Bacterial screening
Choose 30 single bacterium colonies of looking strong from 90s plate, be inoculated on inclined-plane, in 37 DEG C of incubators, cultivate 24 hours, 0--4 DEG C stored refrigerated; With original strain in contrast, adopt conventional herd breeding condition, sodium carboxymethylcellulose is as carbon source and proteolysis circle method screening cellulase-producing and the strong bacterial strain of protease, screening obtains cellulase-producing and strong Strains B. subtilis (Bacillus subtilis) L1 of protease, this bacterial strain cellulase-producing energy force rate original strain improves 25%, produces protease energy force rate original strain and improves 52%.By this bacterial classification in the center preservation of China General Microbiological culture presevation administration committee's common micro-organisms, deposit number is CGMCC No 6012.
This product bacillus subtilis used (Bacillus subtilis) bacterial classification, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, preservation address is positioned at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, (postcode 100101), deposit number is CGMCC No6012.
Research shows by experiment: product of the present invention have good suppression Escherichia coli, staphylococcus aureus, etc. the characteristic of pathogenic bacteria.Adopt the fungistatic effect of cylinder plate method to ganoderma lucidum feedstuff additive product to be studied, test adopts 10 times of water extracts of feed addictive to study.Experimental result is in table 1, and result shows that product of the present invention has the superperformance suppressing pathogenic bacteria growth.
The fungistatic effect of table 1 feedstuff additive product
| Pathogenic bacteria | Staphylococcus aureus | Escherichia coli |
| Antibacterial circle diameter (mm) | 23 | 28 |
The stable performance of product of the present invention, use safety, with other feed addictive all without incompatibility.The addition of product of the present invention in feed is 1-2%, and it completely can substitute antibiotics medicine, and noresidue, free from environmental pollution.After feeding, significantly can strengthen immunity and the premunition of animal body, growth promoting effects, improve daily gain and feed conversion rate, and have resisting stress, antioxidant effect; There is stronger prevention effect to intestinal bacteriosis simultaneously.According to test, the aquatic products of feeding feed addictive of the present invention, quality improves, and meet the greenization production requirement of animal food, Social benefit and economic benefit is very remarkable.
The Chinese tradition edible mushroom that product of the present invention is produced with biological fermentation process is for raw material, constant product quality is controlled, product noresidue in vivo, non-environmental-pollution, without the resistance to the action of a drug, and immunity can be improved, there is strong bacteriostasis, can culture benefit be improved.Product meets the requirement of green feed additive completely.
Because glossy ganoderma has higher nutritive value and bacteria resistance function, the immunity of animal can be strengthened, and noresidue, environmentally safe.Produce glossy ganoderma by biofermentation technique and be processed as feed addictive, meeting the developing direction of current feed addictive completely, is a kind of green feed additive.
Embodiment 1 (feedstuff additive product)
The invention provides a kind of nutrient feed additive product;
Parts by weight are composed as follows:
Stachyose 2, soya-bean polypeptides 5, Chinese angelica root extract 4, lentinan 4, bacillus subtilis powder 20, Lactobacillus plantarum bacterium powder 20, ginger powder 4, Ganoderma Lucidum solid fermentation culture 5.
Preparation method, is prepared by granulator granulation after said components proportionally being mixed.
Lactobacillus plantarum is CGMCC 9405, and bacillus subtilis (Bacillus subtilis) is CGMCC6012.
Preparation method is as follows for Ganoderma Lucidum solid fermentation culture:
Slant strains activation culture: be transferred on slant medium by the glossy ganoderma slant strains of preservation, cultivates 110 hours, covers with inclined-plane to mycelia for 25 DEG C;
1. liquid first order seed is cultivated: the one piece of access of above-mentioned glossy ganoderma slant strains picking be equipped with in 500 ml shake flasks of 100 milliliters of culture mediums and carry out first order seed cultivation, condition of culture: rotary shaker 100 revs/min, cultivates 100 hours for 25 DEG C; Cultivation temperature 25 DEG C.
2. liquid two stage seed culture: one-level shake-flask seed is equipped with in 500 ml shake flasks of 100 milliliters of culture mediums with 10% inoculum concentration access and carries out secondary seed cultivation, condition of culture: rotary shaker 100 revs/min, cultivate 130 hours for 25 DEG C; Cultivation temperature 25 DEG C.
3. solid fermentation is cultivated: be equipped with in the 500L solid-state fermentation tank of solid fermentation culture medium after sterilizing by second-level shake flask seed with 8% inoculum concentration access, condition of culture: charge 50%, cultivation temperature 25 DEG C, humidity 85%, ventilation 0.5 (V/V), incubation time 25 days.Stirring 10 minutes is opened every 4 days.Adopt SGF solid-state fermentation tank.
4. fermented and cultured terminates, select fluidized bed drying method, by dry materials to moisture below 7%.Solid fermentation material is pulverized.
In the present invention, solid fermentation culture medium is composed as follows: 67% wheat bran, 1.4% sucrose, 10% corn flour, 16% corncob (after pulverizing), 0.1% peptone, 2% De-fatted soya protein Powder, 0.5% dusty yeast; 50 object gingers are crossed after 3% pulverizing; Medium pH nature.Sterilising conditions: 121 DEG C, 2 hours.
Described Chinese angelica root extract preparation method is as follows:
Radix Angelicae Sinensis added Radix Angelicae Sinensis 5 times of weight absolute ethyl alcohol Soakage extraction after pulverizing 45 mesh sieves, control temperature 35 DEG C, adjust after 3 hours temperature be 57 DEG C 1.5 hours, extract is concentrated, drying obtains ethanol extract; Add 80 DEG C of hot water in Radix Angelicae Sinensis residue after alcohol extract, hot water addition is 3 times of Radix Angelicae Sinensis residue weight, 40 minutes processing times, extracts 2 times continuously, by spraying dry after extract Vacuum Concentration, obtains hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, crosses 60 mesh sieves, obtain angelica extract.
Described bacillus subtilis is bacillus subtilis CGMCC6012, and in freeze-dried vaccine powder, number of viable is 6 × 10
8individual/g.
Described composite feed additive is obtained by following methods:
After pulverizing, Ganoderma Lucidum culture, stachyose, soya-bean polypeptides, Chinese angelica root extract and bacillus subtilis freeze-dried vaccine powder are proportionally mixed to get feed addictive.
Soya-bean polypeptides employing Heilungkiang flood icing the more health food Co., Ltd sells soya-bean polypeptides product.
Embodiment 2
The invention provides a kind of nutrient feed additive product;
Parts by weight are composed as follows:
Stachyose 1, soya-bean polypeptides 10, Chinese angelica root extract 5, lentinan 2, bacillus subtilis powder 23, Lactobacillus plantarum bacterium powder 25, ginger powder 2, Ganoderma Lucidum solid fermentation culture 8.
Lactobacillus plantarum is CGMCC 9405, and bacillus subtilis (Bacillus subtilis) is CGMCC6012.
Embodiment 3
The invention provides a kind of nutrient feed additive product;
Parts by weight are composed as follows:
Stachyose 3, soya-bean polypeptides 3, Chinese angelica root extract 5, lentinan 2, bacillus subtilis powder 23, Lactobacillus plantarum bacterium powder 25, ginger powder 2, Ganoderma Lucidum solid fermentation culture 3.
Lactobacillus plantarum is CGMCC 9405, and bacillus subtilis (Bacillus subtilis) is CGMCC6012.
Embodiment 4
The invention provides a kind of nutrient feed additive product; Parts by weight are composed as follows:
Stachyose 3, soya-bean polypeptides 3, Chinese angelica root extract 5, lentinan 2, bacillus subtilis powder 23, Lactobacillus plantarum bacterium powder 15, ginger powder 5, Ganoderma Lucidum solid fermentation culture 8.
Lactobacillus plantarum is CGMCC 9405, and bacillus subtilis (Bacillus subtilis) is CGMCC6012.
Embodiment 5 production method is substantially with example 1
The invention provides a kind of nutrient feed additive product;
Parts by weight are composed as follows:
Stachyose 3, soya-bean polypeptides 10, Chinese angelica root extract 5, lentinan 2, bacillus subtilis powder 16, Lactobacillus plantarum bacterium powder 15, ginger powder 2, Ganoderma Lucidum solid fermentation culture 8.Lactobacillus plantarum is CGMCC 9405, and bacillus subtilis (Bacillus subtilis) is CGMCC6012.
Product result of use
The result of use test of example 1 feed addictive of the present invention in weanling pig
Test method:
Subordinate plant of experimental animal selection Ningxia feed corporation,Ltd average weight is the healthy weanling pig 120 of (7.16 ± 0.15) kg, and statistical analysis weight differences is not remarkable.Adopt single-factor Randomized Designs, by 120 healthy weanling pigs, by male and female half and half, be divided into 2 groups (control group and test group), often organize 6 repetitions, each repetition 10 pigs.Test group adds the obtained product of the present invention of embodiment 1, and control group does not add product of the present invention.Feed continuously 30 days, compared with control group, use invention feed addictive can obtain following effect:
| Project | Control group | Test group | |
| Starting weight (kg) | 7.09 | 7.23 | |
| End heavy (kg) | 18.34 | 19.95 | |
| Daily gain (g/d ﹒ head) | 375 | 424 | |
| Daily ingestion amount (g/d ﹒ head) | 607.5 | 627.5 | |
| Feedstuff-meat ratio | 1.62 | 1.48 | |
| Diarrhea rate (%) | 14 | 2 |
Result of the test shows, in weanling pig daily ration, add this product, and the daily gain of piglet improves a lot than control group; Feedstuff-meat ratio has obvious reduction; Diarrhea rate reduces significantly.
The result of use test of example 1 feed addictive of the present invention in milking sow
The feeding experiment of 21 days has by a definite date been carried out on Ningxia feed corporation,Ltd subordinate pig farm.Test adopts single factor test contrast design, random selecting 30 healthy, farrowing head number is close with birth counterpoise, parity is the milking sow of 2 or 3 tires, be divided at random 2 groups (i.e. test group and control groups), often group 15 repetitions.Wherein: test group daily ration adds this product be prepared into by embodiment 2, and control group does not add product of the present invention.Record weaned piglet weight, diarrhea rate, the death rate.
| Project | Test group | Control group | Deviation |
| Number born alive (head number) | 10.9 | 11.0 | -- |
| Birth counterpoise (kg) | 1.46 | 1.48 | -- |
| 21 days small weaning pigs (head) | 10 | 8.30 | 20.4％ |
| 21 days weight of weaning litters (kg) | 66.4 | 44.4 | 49.5％ |
| Wean counterpoise (kg) in 21 days | 6.64 | 5.35 | 24.1％ |
| Head net gain (kg) | 5.18 | 3.87 | 33.8％ |
| Head daily gain (g) | 246 | 184 | 33.6％ |
| Grice diarrhoea rate (%) | 6.1 | 14.3 | -57.3％ |
| The death rate (%) | 1.6 | 5.4 | -70.3％ |
Test shows that product of the present invention can improve sow and piglet body immunity, reduces antibiotic dosage, increases cultivation quality and benefits.
Claims (6)
1. a nutrient feed additive product, parts by weight are composed as follows: stachyose 1-3, soya-bean polypeptides 3-10, Chinese angelica root extract 3-5, lentinan 2-6, bacillus subtilis powder 16-23, Lactobacillus plantarum bacterium powder 15-25, ginger powder 2-5, Ganoderma Lucidum solid fermentation culture 3-8.
2. nutrient feed additive product as claimed in claim 1, Lactobacillus plantarum is CGMCC 9405, and bacillus subtilis (Bacillus subtilis) is CGMCC6012.
3. nutrient feed additive product as claimed in claim 1, preparation method is as follows for Ganoderma Lucidum solid fermentation culture: slant strains activation culture: be transferred on slant medium by the glossy ganoderma slant strains of preservation, cultivate 96-120 hours, cover with inclined-plane to mycelia for 22-28 DEG C; Optimum culturing temperature 25 DEG C; Liquid Culture is inoculated into solid fermentation and cultivates, and adopts SGF solid-state fermentation tank; Fermented and cultured terminates, and selects the multiple drying means such as fluid bed, by dry materials to moisture below 7%.Solid fermentation material is pulverized; In above-mentioned solid fermentation culture medium, be added with mass ratio is after 2-6% pulverizes, and crosses 40-50 object ginger.
4. nutrient feed additive product as claimed in claim 1, described Chinese angelica root extract preparation method is as follows: Radix Angelicae Sinensis is pulverized, after crossing 40-50 mesh sieve, add Radix Angelicae Sinensis 3-6 times of weight absolute ethyl alcohol Soakage extraction, control temperature 30-45 DEG C, adjusting temperature after 2-4 hour is 55-60 DEG C of 1-2 hour, and extract is concentrated, drying obtains ethanol extract; Add 75-85 DEG C of hot water in Radix Angelicae Sinensis residue after alcohol extract, hot water addition is 2-4 times of Radix Angelicae Sinensis residue weight, and processing time 30-50 minute, extracts 2-3 time continuously, by spraying dry after extract Vacuum Concentration, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, crosses 60 mesh sieves, obtain angelica extract.
5. nutrient feed additive product as claimed in claim 1, parts by weight are composed as follows: stachyose 2, soya-bean polypeptides 5, Chinese angelica root extract 4, lentinan 4, bacillus subtilis powder 20, Lactobacillus plantarum bacterium powder 20, ginger powder 4, Ganoderma Lucidum solid fermentation culture 5.
6. nutrient feed additive product as claimed in claim 1, parts by weight are composed as follows: stachyose 1, soya-bean polypeptides 10, Chinese angelica root extract 5, lentinan 2, bacillus subtilis powder 23, Lactobacillus plantarum bacterium powder 25, ginger powder 2, Ganoderma Lucidum solid fermentation culture 8.
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| CN106901015A (en) * | 2016-04-01 | 2017-06-30 | 陈伟 | Feed addictive containing active probiotic and its production and use |
| CN107259165A (en) * | 2017-08-03 | 2017-10-20 | 宿松县玖索科技信息有限公司 | A kind of suckling animals feed and preparation method thereof |
| CN109548989A (en) * | 2018-09-27 | 2019-04-02 | 安徽省强家庄生态农业有限公司 | Young ox immunity feed of a kind of raising and preparation method thereof |
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