CN103340277A - Feeding microecological additive and compound feed containing same - Google Patents
Feeding microecological additive and compound feed containing same Download PDFInfo
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- CN103340277A CN103340277A CN2013102259105A CN201310225910A CN103340277A CN 103340277 A CN103340277 A CN 103340277A CN 2013102259105 A CN2013102259105 A CN 2013102259105A CN 201310225910 A CN201310225910 A CN 201310225910A CN 103340277 A CN103340277 A CN 103340277A
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Abstract
The invention discloses a feeding microecological additive and applications thereof, specifically to a composite microecological preparation containing a plurality of probiotics and prebiotics, and applications of the preparation. The preparation is prepared by mixing bacillus licheniformis CGMCC No. 7030, bacillus subtilis, enterococcus faecalis, saccharicterpenin and a carrier according to a certain ratio. The protrude advantages comprises: under the combined action of bacillus licheniformi, the bacillus subtilis powder, a lactobacillus powder and saccharicterpenin, intestinal structure and flora of animals are improved; the microecological additive can help to improve forage utilization rate, promote animal growth, improve output, raise immunity and partially substitute antibiotic, and thus animal product quality is improved; and the microecological additive is beneficial for animal and human health.
Description
Technical field
The invention belongs to field of animal feed, be specifically related to a kind ofly form feed addictive and contain its batch by bacillus licheniformis, bacillus subtilis, enterococcus faecalis, saccharicter-penin and carrier mixed preparing.
Background technology
Along with being rooted in the hearts of the people day by day of the strategy of sustainable development, the raising of scientific and technological progress, economic development and living standards of the people, in recent years, pollution-free food, pollution-free food have become people's consumption to be pursued.Yet the additive that still exists additives such as antibiotic, hormone at present in culturing industry on a large scale uses, though these materials have improved the total amount of animal products, and, its residual injury that will cause human body in food.
Symphysis unit is the probiotics that probiotic and prebiotics are united use, is characterized in bringing into play simultaneously the effect of probio and prebiotics.It is to take oxygen, flora adjustment by force, strengthen enzymatic activity and improve immunologic function to reach control animal intestinal pathogenicity diarrhoea and growth promoting function by biology that probiotic mainly acts on.Prebiotics is the growth of the interior beneficial bacterium of stimulation of host animal alimentary canal optionally, thereby animal is produced favourable effect, comprises compound sugar, little algae and saccharicter-penin etc.
Summary of the invention
In order to address the above problem, the invention provides a kind of feeding micro-ecological additive and application thereof.
Feeding micro-ecological additive provided by the invention, this additive contains: bacillus licheniformis, bacillus subtilis, lactic acid bacteria and saccharicter-penin, make its performance synergy, can effectively improve animal health condition, improve food conversion ratio.
Wherein, described bacillus licheniformis can be the various bacterial strains that this area is fit to, and is preferably CGMCC No.7030.
Wherein, described enterococcus faecalis can be the various bacterial strains that this area is fit to, and is preferably CGMCC No.5092.
Wherein, described carrier can be any carrier that this area is used for additive, is preferably in maize cob meal, powdered rice hulls, soluble starch, wheat bran, defatted rice bran, corn protein powder, the stone flour one or more.
In the working of an invention scheme, it is 1～5:0.01～0.1:0.1～1:3～5:7～15 according to weight ratio that described additive contains bacillus licheniformis, bacillus subtilis, lactic acid bacteria, saccharicter-penin and carrier, saccharicter-penin and carrier, and wherein bacterium powder viable count is respectively 2.0～5.0 * 10
9Cfu/g, 1.0～5 * 10
11Cfu/g and 2.0～5.0 * 10
10Cfu/g.
In embodiment preferred of the present invention, it is 3:0.04:0.8:4:9 according to weight ratio that described additive contains bacillus licheniformis, bacillus subtilis, lactic acid bacteria, saccharicter-penin and carrier, saccharicter-penin and carrier, and wherein bacterium powder viable count is respectively 2.0～5.0 * 10
9Cfu/g, 1.0～5 * 10
11Cfu/g and 2.0～5.0 * 10
10Cfu/g.
The present invention also provides the application of described additive in improving the weanling pig production performance, and making the addition of described additive in basal diet is 0.1wt ‰～1wt ‰, preferred 0.5wt ‰.
In basal diet, add additive of the present invention, can significantly improve the weanling pig daily gain, reduce feedstuff-egg ratio and diarrhea rate.
Bacillus licheniformis of the present invention (Bacillus licheniformis) obtains for the applicant separates from the duck enteron aisle.Its vegetative cell is shaft-like, and its gemma is oval or long tubular, the oval gemma of giving birth in the generation, and sporangiocyst expands.Gram is positive.Form white at beef-protein medium, opaque, irregular bacterium colony, the edge is hair-like, corrugationless.The catalase positive can be utilized propionate.Can produce the various active enzyme, as protease, cellulase etc., improve efficiency of feed utilization, strengthen the activity of animal digestion enzyme, promote the growth of cultivated animals.Bacillus licheniformis of the present invention (Bacillus licheniformis) applicant has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on December 25th, 2012, be called for short CGMCC, address: No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, deposit number is CGMCC No.7030, and this bacterial strain is announced.
Enterococcus faecalis of the present invention (Enterococcus faecalis) obtains for the applicant separates from Radix Polygalae Crotalarioidis, and its biological property is as follows: the bacterium colony rounding, and smooth surface, opaque, milky, the edge is smooth, gram-positive bacteria; Single thalline is spherical or ellipticity.Enterococcus faecalis of the present invention is through simulated gastric fluid, bile fluid and stable on heating screening; Again through enzymatic productivity screening and bacteriostasis screening, can tolerate pH2.0,1% pepsic simulated gastric fluid, can tolerate 0.3% artificial bile fluid, can tolerate 85 ℃ pelleting temperature, also can suppress enteropathogenic E K88, K99 and staphylococcus aureus, have the ability of mould in stronger product acid and inhibition dregs of beans, cotton dregs, the maize straw.Enterococcus faecalis of the present invention (Enterococcus faecalis) applicant has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 26th, 2011, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.5092, and this bacterial strain is announced.
Bacillus licheniformis CGMCC No.7030 of the present invention, can produce highly active cellulase and protease by fermentation, add the decomposition that can increase compositions such as protein in the feed, cellulose and hemicellulose in the precession thing feed, improve the feed nutrient utilization rate.
Bacillus licheniformis in the micro-ecological additive of the present invention, bacillus subtilis bacterium powder, lactic acid bacteria bacterium powder, saccharicter-penin acting in conjunction, can improve animal intestinal structure and flora, can improve efficiency of feed utilization, promote growth of animal, improve output, improve immunity, part substitutes antibiotic, thereby improves the animal product quality.Be of value to animals and human beings class health.
The specific embodiment
Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
The mensuration that embodiment 1 bacillus licheniformis CGMCC No.7030 fermentation broth enzyme is lived
To be inoculated on the beef extract-peptone solid medium for the examination bacterium, picking bacterial classification behind 30 ℃ of cultivation 24h is suspended from 0.85% physiological saline, and the blood counting chamber counting is regulated concentration about 5.0 * 10
7Individual/mL inserts bacteria suspension 2mL in the 100mL triangular flask that 20mL product enzyme culture medium is housed, 30 ℃ of natural supply oxygen, and 170r/min condition bottom fermentation is cultivated 24h, and liquid fermentation liquid is centrifugation 15min under the 5000r/min condition, gets supernatant, is crude enzyme liquid.
Cellulase produces the enzyme culture medium prescription: peptone 0.9%, yeast extract 1.0%, CMC-Na0.5%, pH8.0.
Protease produces the enzyme culture medium prescription: peptone 1.0%, yeast extract 0.5%, glucose 1.0%, soluble starch 0.5%, KH
2PO40.2%, MgSO
4〃 7H
2O0.05%, CaCl
2〃 2H
2O0.02%, pH7.2.
(1) cellulase activity is measured
Cellulase activity is measured: according to GB NY/T912-2004 drawing standard curve, adopt the cellulase activity in the DNS method mensuration zymotic fluid.Containing 1%(w/v with 1mL) the pH8.0 phosphate buffer of CMC-Na is substrate, adds the 0.2mL crude enzyme liquid, 50 ℃ of insulation 30min, add 2.5mL DNS reagent then, boiling water bath 5min is settled to 5.0mL after being cooled to room temperature, utilizes the absorbance at spectrophotometric determination wavelength 540nm place; Add above-mentioned substrate reactions liquid as blank to boil the deactivation crude enzyme liquid, with substrate solution as negative control; 1mL enzyme liquid per minute is produced the 1g glucose amount be defined as 1 enzyme activity unit, U/mL.
After measured, the cellulase activity of the fermentation crude enzyme liquid of bacillus licheniformis CGMCC7030 is: 230.5U/mL.
(2) prolease activity is measured
The mensuration of prolease activity: adopt the folin's methods drawing standard curve in the GB SB/T10317-1999 protease activity amylograph.Sample determination: get 3 in 15 * 100mm test tube, numbering 1,2,3, add crude enzyme liquid 1mL in every pipe, place 40 ℃ of water-bath preheating 2min, each adds 2% casein solution (pH7.2) 1mL through same preheating again, accurately be incubated 10min, after time arrives, respectively add 0.4mol trichloroacetic acid 2m L immediately again, with cessation reaction, continue to place water-bath to be incubated 20min, make the centrifugal or filtration of residual protein post precipitation, get 3 in 15 * 150mm test tube then in addition, numbering 1,2,3, add filtrate 1mL in every pipe, add 0.4mol sodium carbonate 5mL again, the Folin reagent 1mL that has diluted shakes up, and measures O D value behind 40 ℃ of insulation color development 20min under 540nm.3 in test tube is also got in blank test, numbering (1), (2), and (3), assay method is the same, only adds 0.4mol trichloroacetic acid 2mL before adding 2% casein earlier, makes enzyme deactivation, adds 2% casein again.
The prolease activity unit definition is: 1mL enzyme liquid is at 40 ℃, and under the condition of pH7, the enzyme amount that the per minute caseinhydrolysate produces 1 μ g tyrosine is an enzyme activity unit, represents with (U/ml).
The compound method of Folin reagent (Folin reagent): in 200mL ground reflux, add sodium tungstate (Na
2WO
42H
2O) 100g, sodium molybdate (Na
2MO
4〃 2H
2O) 25g, distilled water 700mL, 85% phosphoric acid 50mL, concentrated hydrochloric acid 100mL, slow fire backflow 10h.Remove condenser, add lithium sulfate (Li
2SO
4) 50g, distilled water 50m L, mixing adds a few drop of liquid bromines of people, boils 15min again, removes color to expel residual bromine, and it is yellow but not green that solution should be.If solution still has green, need add several Australia's liquid again, boil again and remove it.After the cooling, be settled to 1000mL, filter with bacterial filter (2.2 μ m), place brown bottle to preserve.This solution adds 2 times of distilled water dilutings when using.The Folin reagent that has diluted.
After measured, the prolease activity of every milliliter of bacillus licheniformis CGMCC7030 fermentation crude enzyme liquid is 1500U/mL.
The product enzyme test shows, bacillus licheniformis CGMCC No.7030 of the present invention, can produce highly active cellulase and protease by fermentation, add the decomposition that can increase compositions such as protein in the feed, cellulose and hemicellulose in the precession thing feed, improve the feed nutrient utilization rate, reduce concentrations of nutrient in the animal wastes simultaneously, alleviate the pollution to environment, have very high feeding value.
The preparation of embodiment 2 bacillus licheniformis CGMCC No.7030 freeze-dried powders
The preparation method of bacillus licheniformis bacterium powder comprises following step
(1) dull and stereotyped cultivation rejuvenation: the bacillus licheniformis bacterial classification inoculation on plating medium, in 30 ℃ of cultivation 18h, is made the bacillus licheniformis rejuvenation, and forms single bacterium colony, and picking list bacterium colony is cultivated 24h for 30 ℃ on the inoculation plating medium;
Described plating medium prescription is: tryptone 1%, yeast extract 0.5%, sodium chloride 1%, agar 2%, pH7.0 ± 0.2.
(2) preparation of primary seed solution: the bacillus licheniformis bacterial classification that step (1) is cultivated is forwarded in the test tube that the 10mL culture medium is housed, and 30 ℃ of shaking tables are cultivated 16h, and rotating speed is 170rpm, makes to be in the logarithm later stage, gets primary seed solution;
(3) preparation of secondary seed solution: it is in the conical flask of 250ml that the primary seed solution of step (2) preparation is inoculated in the capacity that the 75ml culture medium is housed by 1% inoculum concentration, and 30 ℃ of constant temperature shaking tables are cultivated 16h, and rotating speed is 170rpm, gets secondary seed solution;
The seed culture based formulas is: tryptone 1%, yeast extract 0.5%, sodium chloride 1%, pH7.0 ± 0.2.
(4) preparation of the lichen bacillus ferments liquid: be 1% to be inoculated in the fermentation tank of 100L the seed liquor in the step (3) by inoculum concentration, sample-loading amount 20%, 30 ℃ of temperature, the aerobic gemma production rate that is cultured to of rotating speed 250rpm is more than 80%, and viable count is 10
10More than the CFU/ml, then put a jar termination fermentation, get the lichen bacillus ferments liquid;
Described fermentation medium is: wheat bran 2%, corn flour 1%, dregs of beans 2%, ammonium sulfate 2%, magnesium sulfate 0.03%; Ammonium citrate 0.5%, pH are 7.0-7.4.
(5) preparation of bacillus licheniformis freeze-dried vaccine powder: after the zymotic fluid of step (4) preparation is centrifugal; (the frozen-dried protective agent prescription is the freeze drying protectant of adding 10%: skimmed milk power 10% in bacterium mud; lactose 10%; glycerine 0.5%; vitamin C 0.2%, distilled water 79.3%), the mixing postlyophilization; obtain the bacillus licheniformis bacterium powder of moisture＜5%, viable count is about 40,000,000,000 in the freeze-dried vaccine powder.
The preparation of embodiment 3 bacillus subtilis freeze-dried powders
1, the preparation of bacillus subtilis (Bacillus subtilis) CGMCC No.6511 bacterium powder
1) preparation of bacillus subtilis seed liquor: preservation bacterial classification inoculation in test tube slant to the test tube that the 15mL culture medium is housed, is cultivated 16-20h in 37 ℃ of constant temperature shaking tables, and rotating speed is 225rpm, treats that viable count reaches 10
7CFU/mL is inoculated in the 250mL conical flask that the 100mL seed culture medium is housed by 1%, cultivates 16-20h in 37 ℃ of constant temperature shaking tables, and shaking speed is 225rpm, treats that viable count reaches 10
7CFU/mL, standby as seed liquor.
Seed culture medium is: glucose, 20.0g/L; Peptone, 10.0g/L; Dusty yeast, 4.0g/L; Sodium chloride, 5g/L; Distilled water adds to 1000mL, pH7.0 ± 0.2.
2) liquid deep layer fermenting is cultivated
Select for use the 5L vertical digester to carry out liquid fermentation and culture.Be 1% with the inoculum concentration of bacillus subtilis seed liquor, behind 20%, 37 ℃ of aerobic cultivation 20h of liquid amount, measure every milliliter of viable count in the bacterium liquid, treat that viable count reaches 10
8More than the CFU/mL, the gemma formation rate arrives 80%, and warping finishes fermented and cultured, and (defoamer is vegetable oil, addition 1%, unit: g/L).
Fermentation medium is composed as follows: peptone, 50g/L; Glucose 10g/L; Dusty yeast 17g/L; Compound sugar 3g/L; Distilled water adds to 1000mL, pH7.0 ± 0.2.
3) freeze-dried vaccine powder preparation
By centrifugal, concentrated, the production routines such as adding freeze drying protectant, vacuum freeze drying and the check of bacterium powder viable count of zymotic fluid, finally prepare the powdery active bacteria formulation.
4) finished product detection
Get the powdery active bacteria formulation 1g that has prepared, measure viable count in every gram bacterium powder by international NY/T1461-2007 method, record that viable count is about 1.0 * 10 in the bacterium powder
11Cfu/g.
The preparation of embodiment 4 enterococcus faecalis freeze-dried powders
1) dull and stereotyped cultivation rejuvenation: enterococcus faecalis CGMCC No.5092 bacterial classification inoculation on the MRS plating medium, in 37 ℃ of cultivation 24h, is made the rejuvenation of enterococcus faecalis bacterium, and forms single bacterium colony; Picking list colony inoculation is cultivated 36h in 37 ℃ on slant medium;
2) preparation of first order seed: the enterococcus faecalis slant strains that step 1) is cultivated is transferred in the 500mL triangular flask that 250ml～300ml MRS culture medium is housed, and in 37 ℃ of cultivation 16h, rotating speed 150rpm makes it be in the logarithm middle and later periods, is first order seed;
3) preparation of secondary seed: with step 2) the Bacillus acidi lactici first order seed of Pei Yanging is transferred in the 2.0L triangular flask that 1.5L MRS culture medium is housed, and in 37 ℃ of static cultivation 16h, is secondary seed solution;
4) preparation of fermentation liquid: the secondary seed solution of step 3) preparation is inoculated into according to 5% inoculum concentration 12～16M is housed
3In the fermentation tank of fermentation medium, 37 ℃ of temperature, rotating speed 160rpm, tank pressure 0.05Mpa cultivates 16h, to viable count be 2～5 * 10
9Cfu/ml, stuck fermentation;
Described fermentation medium is: glucose 1～2%, soy peptone 1～2%, ammonium sulfate 0.5～1.0%, sodium chloride 0.2～0.8%, magnesium sulfate 0.01～0.05%;
5) preparation of enterococcus faecalis bacterium powder: with the zymotic fluid of step 4) preparation; 5000rpm is centrifugal; obtain bacterium mud; the weight/volume percent of adding and bacterium mud is 20% freeze drying protectant; mixing; in-40 ℃ of freeze dryings, obtain the enterococcus faecalis bacterium powder of moisture＜5%, record that viable count is about 2.0 * 10 in the bacterium powder
10Cfu/g.
Described freeze drying protectant is: the mixture of skimmed milk power, glycerine, sucrose, maltodextrin and sodium glutamate, according to: skimmed milk power: glycerine: sucrose: maltodextrin: the ratio of sodium glutamate=2: 0.5～1.0: 0.5～1.0: 0.5～1.5: 0.5～1.0 mixes.
Embodiment 5
The feeding symphysis of the present invention unit additive adopts following raw material to make: bacillus licheniformis, bacillus subtilis, enterococcus faecalis, saccharicter-penin and carrier, saccharicter-penin and maize cob meal are that 3:0.04:0.8:4:9 mixes according to weight ratio, can obtain additive of the present invention wherein bacterium powder viable count be respectively 5.0 * 10
9Cfu/g, 1.0 * 10
11Cfu/g and 2.0 * 10
10Cfu/g.
Using method: with the addition of this additive according to weight 0.5 ‰, add in the basal diet of piglet, mix, routine is fed, and gets final product.
Embodiment 6
The feeding symphysis of the present invention unit additive adopts following raw material to make: bacillus licheniformis, bacillus subtilis, lactic acid bacteria, saccharicter-penin and powdered rice hulls are that 3.5:0.04:0.7:3.2:9 mixes according to weight ratio, can obtain additive of the present invention.Wherein bacterium powder viable count is respectively 5.0 * 10
9Cfu/g, 1.0 * 10
11Cfu/g and 2.0 * 10
10Cfu/g.
Using method: with the addition of this additive according to weight 0.6 ‰, add in the basal diet of laying hen, mix, routine is fed, and gets final product.
Embodiment 7
The feeding symphysis of the present invention unit additive adopts following raw material to make: bacillus licheniformis, bacillus subtilis, lactic acid bacteria, saccharicter-penin and powdered rice hulls are that 3.25:0.05:0.6:3.2:9 mixes according to weight ratio, can obtain additive of the present invention.Wherein bacterium powder viable count is respectively 5.0 * 10
9Cfu/g, 1.0 * 10
11Cfu/g and 2.0 * 10
10Cfu/g.
Using method: with the addition of this additive according to weight 0.4 ‰, add in the basal diet of broiler chicken, mix, routine is fed, and gets final product.
Test example 1 additive is to the influence of piglet production performance
1 experimental design
1.1 experimental animal and material
Test selects for use 72 average weights to hybridize weanling pig for Du * length of (7.85 ± 0.45) Kg * Dasanyuan, is divided into 4 groups immediately by the principle of male and female half and half, and each handles 3 repetitions, and each repeats 6 pigs.Test group is added 0.5 ‰ feeding micro-ecological additives respectively in daily ration, and establish 3 control groups, be respectively, control group 1 is the basal diet of feeding, control group 2 basal diet of feeding adds bacillus subtilis, lactic acid bacteria, the saccharicter-penin probiotics of embodiment 1 preparation, the control group 3 bacillus licheniformis CGMCC No.7030 that feeds.
1.2 the not corn-soybean meal diet of added with antibiotic is adopted in experiment daily ration experiment, with reference to NRC(1998) the swine rearing standard prepares.
1.3 the feeding and management test pig is raised in closed pig house, the cement flooring, the well-ventilated, the house temperature remains on 18-22 ℃, every morning and afternoon each reinforced 2-3 time, guaranteeing has material in the hopper all the time, free choice feeding with freely drink water.Carry out immunity according to the pig farm conventional method.
1.4 testing index
Raise the 5th day of phase in advance, select and stop feeding the 14th day, the 28th day evening 8 of experimental period, as usual supply water, morning next day pig is weighed on an empty stomach one by one, after each issue off-test, be that unit claims surplus material amount to repeat (hurdle), the record feed consumption rate, calculate test early stage (1-14d), later stage (15-28d) and full phase (1-28d) each phase average daily gain (ADG), average daily ingestion amount (ADFI), expect anharmonic ratio (F/G) and diarrhea rate.
Daily gain (ADG): daily gain (g/d)=(the test end weighs-the test initial weight)/test fate.
Daily ingestion amount (ADFI): the feed intake that records every hurdle is calculated the feed intake of every every day;
Feedstuff-meat ratio: the feed that the per unit weightening finish consumes.
Diarrhea rate: record the pig number of elements of diarrhoea in each repetition every day, obtain total diarrhoea number of times of this repetition, with its total raising number of times divided by this repetition, namely draw the diarrhea rate of this repetition.Calculate this processing at last and respectively repeat the mean value of diarrhea rate.The standard of diarrhoea is that ight soil does not form stabilization of solid, and tangible anus tail adherent phenomenon is arranged.
Diarrhea rate (%)=[every hurdle always suffer from diarrhoea head day number of times/(every hurdle piglet number * raising fate)] * 100%
1.5 data analysis
Adopt SPSS16.0 software to carry out data statistic analysis.Result of the test is represented with mean+SD, adopts the one-factor analysis of variance, and the relatively employing LSD multiple ratio of mean value was carried out significance test of difference between each was handled, and was significant difference with p＜0.05.
2 result of the tests
2.1 additive of the present invention is to the influence of weanling pig production performance in early stage
Table 1 additive of the present invention is to the influence of weanling pig production performance in early stage
Annotate: be difference not significantly (p〉0.05), different lowercase person significant differences (p＜0.05) with the line data person that do not have the shoulder
Table 1 is the result show, the test group weight average that increases day by day is higher than three control groups, and difference reaches the level of signifiance (p＜0.05).Daily ingestion amount aspect, test group are higher than the blank group, but difference not significantly (p〉0.05).Test group material anharmonic ratio is lower than control group 1,2, and reaches the level of signifiance (p＜0.05).Diarrhea rate is lower than control group 1 and difference reaches the level of signifiance (p＜0.05).The result shows, adding 0.5 ‰ feeding micro-ecological additive has appreciable impact to weanling pig early stage ADG, F/G and diarrhea rate, and is better than adding respectively bacillus subtilis, lactic acid bacteria, saccharicter-penin or adds bacillus licheniformis CGMCC No.7030.
2.2 additive of the present invention is to the influence of weanling pig later stage production performance
Table 2 additive of the present invention is to the influence of weanling pig later stage production performance
As shown in Table 2, the daily gain (ADG) of test later stage test group is significantly higher than three control groups (p＜0.05).ADFI is at test later stage difference not significantly (p〉0.05).Test group material anharmonic ratio is lower than control group 1,2, and reaches the level of signifiance (p＜0.05).Diarrhea rate be lower than control group 1,3 and difference reach the level of signifiance (p＜0.05).The result shows, adding 0.5 ‰ feeding micro-ecological additive has appreciable impact to weanling pig later stage ADG, F/G and diarrhea rate.
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. a feeding micro-ecological additive is characterized in that, this additive is that 1 ~ 5:0.01 ~ 0.1:0.1 ~ 1:3 ~ 5:7 ~ 15 mixed preparing form by bacillus licheniformis, bacillus subtilis, enterococcus faecalis, saccharicter-penin and carrier according to weight ratio.
2. feeding micro-ecological additive according to claim 1 is characterized in that this additive is formed for the 3:0.04:0.8:4:9 mixed preparing according to weight ratio by bacillus licheniformis, bacillus subtilis, enterococcus faecalis, saccharicter-penin and carrier.
3. feeding micro-ecological additive according to claim 1 is characterized in that, described bacillus licheniformis, bacillus subtilis and enterococcus faecalis are dry powder formulations, and its viable count is respectively 0.1 ~ 1.0 * 10
10Cfu/g, 1.0 ~ 9.0 * 10
11Cfu/g and 2.0 ~ 9.0 * 10
10Cfu/g.
4. feeding micro-ecological additive according to claim 1 and 2 is characterized in that, the preserving number of described bacillus licheniformis is CGMCC No. 7030.
5. feeding micro-ecological additive according to claim 1 and 2 is characterized in that, the preserving number of described bacillus subtilis is CGMCC No. 6511.
6. feeding micro-ecological additive according to claim 1 and 2 is characterized in that, the preserving number of described enterococcus faecalis is CGMCC No. 5092.
7. feeding micro-ecological additive according to claim 1 and 2 is characterized in that, described carrier is one or more in maize cob meal, powdered rice hulls, wheat bran, defatted rice bran, corn protein powder, the stone flour.
8. the application of each described feeding micro-ecological additive of claim 1 ~ 7 in improving livestock and poultry production performance, it is characterized in that, add described feeding micro-ecological additive in basal diet, making the addition of described feeding micro-ecological additive in basal diet is 0.1wt ‰ ~ 1wt ‰.
9. application according to claim 8 is characterized in that, the addition of described feeding micro-ecological additive in basal diet is 0.5wt ‰.
10. the batch that contains each described feeding micro-ecological additive of claim 1 ~ 7.
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| CN103858818A (en) * | 2014-02-07 | 2014-06-18 | 江西大北农科技有限责任公司 | Novel roaster breeding method |
| CN103859236A (en) * | 2014-04-02 | 2014-06-18 | 太仓市永发农场专业合作社 | Chicken feed with high nutrient ingredients |
| CN104171419A (en) * | 2014-08-06 | 2014-12-03 | 南昌傲农生物科技有限公司 | Piglet feed additive and preparation method and use thereof |
| CN104232518B (en) * | 2014-08-27 | 2017-04-26 | 武汉轻工大学 | Bacillus cereus wettable powder as well as preparation method and applications of bacillus cereus wettable powder |
| CN104232518A (en) * | 2014-08-27 | 2014-12-24 | 武汉轻工大学 | Bacillus cereus wettable powder as well as preparation method and applications of bacillus cereus wettable powder |
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| CN106689798A (en) * | 2016-11-17 | 2017-05-24 | 大连理工大学 | Marine synbiotic additive, preparation method thereof, as well as application of marine synbiotic additive in breeding of stichopus japonicus |
| CN106578731A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Marine synbiotics additive and application thereof in stichopus japonicus culture |
| CN109157546A (en) * | 2018-07-27 | 2019-01-08 | 江苏省海洋水产研究所 | A kind of compound micro-ecological preparation of the acute Hepatopancreatic necrosis syndrome of prevention and control Penaeus Vannmei and its application |
| CN110169498A (en) * | 2019-07-04 | 2019-08-27 | 湖南师范大学 | It is a kind of adjust animal and bird intestines length feed addictive and its application |
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