CN106578731A - Marine synbiotics additive and application thereof in stichopus japonicus culture - Google Patents
Marine synbiotics additive and application thereof in stichopus japonicus culture Download PDFInfo
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- CN106578731A CN106578731A CN201611184560.2A CN201611184560A CN106578731A CN 106578731 A CN106578731 A CN 106578731A CN 201611184560 A CN201611184560 A CN 201611184560A CN 106578731 A CN106578731 A CN 106578731A
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- bacillus amyloliquefaciens
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention provides a marine synbiotics additive. The marine synbiotics additive is prepared from the following components: bacillus amyloliquefaciens WB1, marine oligosaccharide prebiotics, a bacteria protectant and a plant source carrier. The invention also provides a preparation method of the marine synbiotics additive. The preparation method comprises the following steps: after taking and adding the bacillus amyloliquefaciens WB1 in the marine oligosaccharide prebiotics and the bacteria protectant, mixing with the plant source carrier; carrying out vacuum freeze drying on an obtained mixture, and preparing the obtained mixture into freeze-dried powder, i.e., the marine synbiotics additive. The invention also provides application of the marine synbiotics additive in stichopus japonicus culture. According to the marine synbiotics additive, and the preparation method and the application thereof, provided by the invention, probiotics and prebiotics are efficiently and scientifically matched and are in synergistic effect, so that the effect of 1+1>2 is expressed; by utilizing the marine synbiotics additive provided by the invention, dual probiotic functions of the bacillus amyloliquefaciens WB1 and the marine oligosaccharide prebiotics can be expressed at the same time, the growth performance and the non-specific immunity response of stichopus japonicus are remarkably increased, and healthy growth of animals is well promoted.
Description
Technical field
The present invention relates to bacillus amyloliquefaciens-marine active oligosaccharide symphysis unit method of production and its in apostichopus japonicus culture
Using.
Background technology
Radix Morinae Bulleyanae is best one kind of quality in Chinese more than 20 kinds of edible Stichopus japonicuss, is ranked first of marine products eight delicacies, with high
Nutritive value and economic worth.It is near that the development of apostichopus japonicus culture industry is swift and violent during the last ten years, national Radix Morinae Bulleyanae yield in 2013 more than 13 ×
104 tons, the output value breaks through 30,000,000,000 yuan, the highest in the aquaculture industry single output value and profit, its development speed and mixed economy
Benefit be all other economic Aquatic product kinds it is incomparable.As Radix Morinae Bulleyanae industry changes to the intensive mode of high density, Radix Morinae Bulleyanae
The health and disease problem of cultivation is increasingly highlighted, and the disease such as skin ulcerative syndrome takes place frequently, and causes production performance to reduce, seriously constrains
Radix Morinae Bulleyanae industry health and sustainable development.Although preventing and treating aquatic animal disease using antibiotic and chemicalses has certain effect
Really, but antibacterial can be caused normal flora in Drug resistance, drug residue and destruction animal and environment occur and many drawbacks such as to balance.
As modern aquaculture industry progressively develops to green, environmentally friendly and nuisanceless direction, the natural green such as probiotic bacteria and prebioticses is raised
Feed additives are because with being balance, promotion growth of animal beneficial to intestinal and water body Tiny ecosystem group and strengthening immunne response, being place
Master tape carrys out many benefits, and green safety, pollution-free, is increasingly becoming the focus of aquaculture field research.
The content of the invention
It is an object of the invention to efficient scientific compatibility probiotic bacteria and prebioticses, so as to significantly improve the growth performance of Radix Morinae Bulleyanae
And nonspecific immune response, to ensure the health sustainable development of Radix Morinae Bulleyanae industry.
In order to achieve the above object, the invention provides a kind of ocean symphysis unit additive, the additive bag is containing from sea
Ocean, marine oligosaccharide class prebioticses --- the brown alga oligose with biological activity, and can be by the use of brown alga oligose as energy substance
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) WB1 of growth.Described bacterial strain WB1 has been filed on preservation,
Concrete preservation information is as follows：
Depositary institution's title：China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC)；
Depositary institution address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica；
Preservation date：On 2 27th, 2014；
Deposit number：CGMCC No.8873；
The bacillus amyloliquefaciens and above-mentioned oligosaccharide class prebiotics scientific compatibility, the ocean symphysis unit additive prepared
Promote the new symphysis unit additive of its growth and enhancing immunity to can be used for apostichopus japonicus culture；
The composition of above-mentioned ocean symphysis unit additive includes：
Bacillus amyloliquefaciens WB1, marine oligosaccharide class prebioticses, bacteria protectant, plant source carrier；
The degree of polymerization of the Marine prebiotics brown alga oligose is 2～10；
The brown alga oligose molecular weight<2000；
The bacteria protectant is defatted milk powder, glycerol, sucrose or gelatin；It is preferred that defatted milk powder；
The plant source carrier is maize cob meal, powdered rice hulls or wheat bran；It is preferred that maize cob meal.
Under optimal way, the ocean symphysis unit additive the ratio of each component is：
Concentration is more than or equal to 1 × 1010Add in the bacillus amyloliquefaciens WB1 bacterium solutions of CFU/mL and account for the bacterium solution quality 5
～8% marine oligosaccharide class prebioticses, account for the bacteria protectant of the bacterium solution quality 5～10% after the solution that obtains and the plant
Thing source carrier compares 1 by volume mass:0.25ml/g mixes, lyophilizing.
Further optimize, bacillus amyloliquefaciens WB1 bacterial concentrations are equal to 1 × 1010CFU/mL, the marine oligosaccharide class of addition
Prebioticses account for 10% that the bacterium solution quality 5%, bacteria protectant accounts for the bacterium solution quality.
Bacillus amyloliquefaciens WB1 of the present invention does not have security risks to Radix Morinae Bulleyanae, and no pathogenicity can be used as the prebiotic of Radix Morinae Bulleyanae
Bacterium uses.
Above-mentioned bacillus amyloliquefaciens WB1 features are as follows：White, translucent, smooth moistening bacterium colony, slightly swells, circular neat
Whole bacterium colony；Gram-positive, cell are in shaft-like, motion, aerobic, are resistant to 1%～10%NaCl concentration, select in brown alga oligose
In selecting property culture medium, colonial morphology is brown, circular, neat in edge, and the most suitable growth pH is 6.5～7.5, optimum growth temperature 25
～35 DEG C.
Present invention also offers the preparation method of above-mentioned ocean symphysis unit additive, comprises the following steps that：
S1, bacillus amyloliquefaciens WB1 bacterium solutions are taken, add marine oligosaccharide class prebioticses, bacteria protectant, mixed
Liquid；
The marine oligosaccharide class prebioticses include the brown alga oligose that the degree of polymerization is 2～10；
The brown alga oligose molecular weight<2000；
The bacteria protectant is defatted milk powder, glycerol, sucrose or gelatin；It is preferred that defatted milk powder.
Under optimal way, the bacillus amyloliquefaciens WB1 bacterial concentrations are more than or equal to 1 × 1010CFU/mL；Preferably equal to
1×1010CFU/mL；
Add the marine oligosaccharide class prebioticses amount account for the bacillus amyloliquefaciens WB1 bacterium solution quality 5～
8%；It is preferred that 5%；
The amount of the bacteria protectant for adding accounts for the 5～10% of the bacillus amyloliquefaciens WB1 bacterium solution quality；It is preferred that
10%.
The preparation method of the bacillus amyloliquefaciens WB1 bacterium solutions is preferably：
Bacillus amyloliquefaciens WB1 is carried out into fermentation enrichment, cultivation temperature is 25～30 DEG C, pH 6.8～7.8, culture 48
～72h, obtains bacillus amyloliquefaciens WB1 fermentation liquids；Adjustment bacillus amyloliquefaciens WB1 fermentation liquid concentration obtains solving starch bud
Spore bacillus WB1 bacterium solutions；
The bacillus amyloliquefaciens WB1 fermentation medium be improve after 2216E oceans culture medium, concrete composition quality
Volume ratio is：
Peptone:Brown alga oligose:Yeast extract:Sand filter seawater=0.3:0.1:0.1:100(g:g:g:g:ml).
This step can adopt colony counting method to adjust the bacillus amyloliquefaciens WB1 fermentation liquid concentration after fermenting.
S3, mixed liquor obtained in step S2 is mixed with plant source carrier；
The plant source carrier is maize cob meal, powdered rice hulls or wheat bran；It is preferred that maize cob meal；
The volume mass ratio preferably 1 that the mixed liquor is mixed with plant source carrier:0.25mL/g.
S4, by mixture vacuum lyophilization 24h～48h obtained in step S3, make lyophilized powder, as ocean symphysis unit
Additive.
The actual measuring and calculating of Jing, the content of bacillus amyloliquefaciens WB1 in ocean symphysis unit additive product obtained in the present invention>
1010CFU/g。
Present invention also offers application of the above-mentioned ocean symphysis unit additive in apostichopus japonicus culture, concretely comprises the following steps：By institute
State ocean symphysis unit additive to be mixed in proportion with Stichopus japonicuss normal feedstuff；
The ocean symphysis unit additive is the 2～5% of the normal feedstuff quality.
The invention has the beneficial effects as follows：
1st, the efficient scientific compatibility probiotic bacteria of the present invention and prebioticses, make the two synergism, play " 1+1>2 " effect,
Bacillus amyloliquefaciens WB1 and marine oligosaccharide class prebioticses dual is played simultaneously can using symphysis unit additive of the present invention
Prebiotic effect, selectively promotes exogenous probiotic bacteria and in animal intestinal is colonized and breeds with endogenouss intestinal beneficial microbes,
The growth protecting of the quick-acting and slow tail effect material prebioticses of collection probiotic bacteria acts on one, plays cooperative effect, promotes animal
Growth, activates host immune system, and directly or indirectly suppresses harmful microbe breeding, so as to significantly improve the growth of Radix Morinae Bulleyanae
Performance and nonspecific immune response, advantageously promote animal health growth.
2nd, the present invention can improve Radix Morinae Bulleyanae intestine microenvironment, promote Radix Morinae Bulleyanae growth performance, improve apostichopus japonicus culture quality with
Benefit, can be used as aquaculture feature green feed additive.
In sum, the present invention is being studied and is holding existing Aquatic product additive base according to intestinal microecology threpsology's principle
On plinth, with reference to latest generation high-flux sequence and micro-ecological bacterial cluster analysiss technology, the specific probiotic bacteria of science screening compatibility and new
Marine oligosaccharide class prebioticses, there is provided a kind of with the efficient symphysis unit preparation for promoting Radix Morinae Bulleyanae growth and activating immune system so as to
Give full play to the prebiotic effect of symphysis unit each component, reach strengthen high density intensive culture pattern under Radix Morinae Bulleyanae growth performance and
The purpose of the ability of disease is resisted, promotes the healthy and stable development of apostichopus japonicus culture industry.
Specific embodiment
The specific embodiment of the present invention is described below：
Embodiment 1
First, prepare ocean symphysis unit additive
Step 1：The fermentation culture bacillus amyloliquefaciens WB1 in the 2216E oceans culture medium after improvement, cultivation temperature is
28 DEG C, pH 7.0, incubation time are 48h, obtain bacillus amyloliquefaciens WB1 fermentation liquids, adjust bacillus amyloliquefaciens WB1 and send out
Zymotic fluid to wherein bacillus amyloliquefaciens WB1 concentration is 1 × 1010CFU/mL, obtains bacillus amyloliquefaciens WB1 bacterium solutions；
Peptone:Brown alga oligose:Yeast extract:Sand filter seawater=0.3:0.1:0.1:100(g:g:g:ml)；
Step 2：Above-mentioned bacillus amyloliquefaciens WB1 bacterium solutions are mixed with marine oligosaccharide class prebioticses and defatted milk powder, is made
Marine oligosaccharide class prebioticses mass concentration is 5%, and defatted milk powder protective agent mass concentration is 10%；
The above-mentioned marine oligosaccharide class prebioticses degree of polymerization is 2～10；
The brown alga oligose molecular weight<2000.
Step 3：Step 2 solution is mixed with plant source carrier maize cob meal, volume mass ratio is 1:0.25ml/g；
Step 4：By mixture vacuum lyophilization 24h obtained in step 3, lyophilized powder is made, as ocean symphysis unit adds
Plus agent.
Two, the preparation of test apostichopus japonicus Selenka feed
Step 1：The selection of Radix Morinae Bulleyanae normal feedstuff, a kind of feed formula provided herein (Ju Ti Pei Fang optionally should be selected)：
Weigh Kelp Powder 2kg, sargassum thunbergii powder 2kg, bean cake powder 1kg, fish flour 1kg, sea mud 3kg, conch meal 0.75kg, compound vitamin and
Mineral 0.25kg；
Wherein, the compound vitamin and mineral and addition composition needed for the normal feedstuff of prior art and quantity will
Ask identical.Here provides a kind of optimal way, and the compound vitamin and mineral composition are (mg/g)：VitaminB10 .5, dimension
Raw element B21.0, nicotinic acid 0.75, vitamin B6 1.5, Vitamin B12 0.05, calcium pantothenate 10, vitamin C 50, choline chloride 200,
Menaquinone K6, vitamin A 0.6, vitamin e1 0, chemical cellulose 723.6；MgSO4-7H2O 100,ZnSO4-7H2O 3,CoCl2-
6H2O 1,MnSO4-H2O 1,Al Cl3-6H2O 0.2,FeSO4-7H2O 30,CuCl2-2H2O 63, Calcium Carbonate 801.8.
Step 2：Feedstuff pretreatment, drying are not higher than 15% to water content, and coarse powder is broken to 100～200 mesh；
Step 3：Before feeding feedstuff daily, by symphysis unit preparation of the present invention according to normal feedstuff weight 5% addition
Abundant spice mixing is carried out, the content of probiotic bacteria wherein in ocean symphysis unit additive>109CFU/g, in normal feedstuff effectively
Content>108CFU/g, then feeds to Radix Morinae Bulleyanae.
Three, raising and the management of Radix Morinae Bulleyanae
The healthy Radix Morinae Bulleyanae for selecting size similar is used as experimental subject, average weight 10g or so.Investigate foregoing invention symphysis unit
Preparation is to the facilitation effect to Radix Morinae Bulleyanae growth and immunity.Experiment carries out adaptability before starting and temporarily supports, all box for breeding 24h aerations,
Using water system, lucifuge, 30% breeding water body is changed daily, low blowdown is inhaled weekly once, in whole experiment process, dissolved oxygen
>=6mg/l, water temperature are held in 16-18 DEG C, and pH 7.9-8.1, salinity are 30-32ppt.The daily set time feeds basis respectively
The feedstuff of feedstuff and addition symphysis unit preparation of the present invention, feeding volume for Radix Morinae Bulleyanae weight 5%, test carries out 60 days.
Four, Radix Morinae Bulleyanae sample collecting and data analysiss
1. growth performance is determined
Survival rate：N2/N1×100
Daily gain：(W2-W1)T-1
Wherein N2And N1It is respectively each parallel Radix Morinae Bulleyanae end head number and initial head number eventually, W2And W1It is respectively each parallel thorn
The whole opisthosoma weight of ginseng and original body mass, T are test natural law.
2. the measure of immune indexes
Coelomocyte of Apostichopus japonicus phagocytic rate is determined
After crystallized purple dyeing and formalin-inactivated, concentration is 109cfu mL-1Staphylococcus aureuses
(Staphyloccocusaureus Rosenbach) is added to one piece 48 together with the coelomocyte of Apostichopus japonicus suspension of certain volume
In orifice plate, inhale repeatedly and beat, be sufficiently mixed cell and bacterium solution.Adjustment cell suspension volume and bacterium solution volume make cell density：Carefully
Strain density=1:50.Lucifuge reaction 1h at 18 DEG C, inhales every 20min and beats once.Then take 40 μ L mixed liquors gently to drip in load glass
On piece, smear is waited to do.Plus 5% formaldehyde (being dissolved in normal saline), phagocyte 3min of the fixed absorption on slide, with distillation
Water is rinsed, and is put.100 phagocyte are counted, the computing formula of phagocytic rate is：
Cell phagocytic rate (%)=phagocytotic phagocyte number/total cellular score × 100 occur altogether
The measure of immune correlation enzyme activity
Superoxide dismutase, lysozyme and nitricoxide synthase are lived, and using test kit, (purchase builds up biology from Nanjing
Graduate School of Engineering) determine.
3. premunition is determined
After 60d culture experiments terminate, each parallel group randomly selects 10 Radix Morinae Bulleyanaes, and every Radix Morinae Bulleyanae body cavity injects 100 μ L
Concentration is 1 × 109cfu mL-1The bacteria suspension of Apostichopus japonicus pathogenic bacteria-Vibrio splindidus, continuous injection 3 days, period does not carry out feedstuff throwing
Hello, after observing two weeks, statistics Radix Morinae Bulleyanae cumulative life-incidence (cumulative incidence rate=morbidity Radix Morinae Bulleyanae number/Radix Morinae Bulleyanae sum).
Impact of the symphysis unit of the present invention to Radix Morinae Bulleyanae growth performance and nonspecific immunity power is as shown in table 1 and table 2：
Table 1. feeds impact (n=30) of the symphysis unit of the present invention to Radix Morinae Bulleyanae growth performance
Table 2. feeds impact of the symphysis unit of the present invention to Radix Morinae Bulleyanae immunity and premunition
Result of the test shows：It is extra in Radix Morinae Bulleyanae normal feedstuff to add symphysis unit of the present invention, Radix Morinae Bulleyanae can be significantly improved special
Determine the growth performances such as rate of growth, effectively strengthen coelomocyte of Apostichopus japonicus phagocytic rate, superoxide dismutase, lysozyme and nitric oxide
Synthase activity, and cumulative life-incidence of the Radix Morinae Bulleyanae under Vibrio splindidus stress can be significantly reduced.Therefore, in Radix Morinae Bulleyanae normal feedstuff
Addition symphysis unit of the present invention can remarkably promote Radix Morinae Bulleyanae growth performance and nonspecific immunity power and premunition in right amount, can be used as thorn
Ginseng novel green feed additive is used.
The present invention is obtained feed additive using the pattern of symphysis unit, and symphysis unit is probiotic bacteria and prebioticses scientific compatibility system
Into biological preparation, its essence is not the simple superposition of two kinds of products, but the change of a matter.It is specific by efficient compatibility
Probiotic bacteria and prebioticses, targetedly promote exogenous probiotic bacteria in animal intestinal to be colonized and grow, and selective stimulating
The propagation of endogenous profitable strain, adjusts immune system, improves defence capability of the body to pathogenic microorganism, improves animal intestinal micro-
Ecogroup system balance, plays dual parts of probiotic bacteria and prebioticses, reaches " 1+1>2 " effect, it is dynamic so as to advantageously promote
The growth of thing and health.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope of present disclosure, technology according to the present invention scheme and its
Inventive concept equivalent or change in addition, should all be included within the scope of the present invention.
Claims (9)
1. a kind of ocean symphysis unit additive, it is characterised in that composition includes：
Bacillus amyloliquefaciens WB1, marine oligosaccharide class prebioticses, bacteria protectant, plant source carrier；
The marine oligosaccharide class prebioticses are the brown alga oligose of 2～10 degree of polymerization；
The brown alga oligose molecular weight<2000；
The bacteria protectant is defatted milk powder, glycerol, sucrose or gelatin；
The plant source carrier is maize cob meal, powdered rice hulls or wheat bran.
2. ocean symphysis unit additive according to claim 1, it is characterised in that the ratio of each component is：
Concentration is more than or equal to 1 × 1010Add in the bacillus amyloliquefaciens WB1 bacterium solutions of CFU/mL and account for the bacterium solution quality 5～8%
Marine oligosaccharide class prebioticses, account for the bacteria protectant of the bacterium solution quality 5～10% after the solution that obtains and the plant source
Carrier compares 1 by volume mass:0.25ml/g mixes, lyophilizing.
3. symphysis unit additive in ocean according to claim 1 or claim 2, it is characterised in that
The plant source carrier is maize cob meal；
The bacteria protectant is defatted milk powder.
4. the preparation method of symphysis unit additive in ocean described in claim 1, it is characterised in that comprise the following steps that：
S1, bacillus amyloliquefaciens WB1 bacterium solutions are taken, add marine oligosaccharide class prebioticses, bacteria protectant, obtain mixed liquor；
The marine oligosaccharide class prebioticses are the brown alga oligoses that the degree of polymerization is 2～10；
The brown alga oligose molecular weight<2000；
The bacteria protectant is defatted milk powder, glycerol, sucrose or gelatin；
S2, mixed liquor obtained in step S1 is mixed with plant source carrier；
The plant source carrier is maize cob meal, powdered rice hulls or wheat bran；
S3, by mixture vacuum lyophilization 24h～48h obtained in step S2, make lyophilized powder, as ocean symphysis unit addition
Agent.
5. the preparation method of ocean symphysis unit additive according to claim 4, it is characterised in that the solution starch spore bar
The preparation method of bacterium WB1 bacterium solutions is：
Bacillus amyloliquefaciens WB1 is carried out into fermentation enrichment, cultivation temperature is 25～30 DEG C, pH 6.8～7.8, culture 48～
72h, obtains bacillus amyloliquefaciens WB1 fermentation liquids；Adjustment bacillus amyloliquefaciens WB1 fermentation liquid concentration obtains solving starch spore
Bacillus WB1 bacterium solutions；
The bacillus amyloliquefaciens WB1 fermentation medium be improve after 2216E oceans culture medium, concrete composition quality volume
Than for：
Peptone:Brown alga oligose:Yeast extract:Sand filter seawater=0.3:0.1:0.1:100(g:g:g:ml).
6. the preparation method of ocean symphysis unit additive according to claim 4, it is characterised in that
The bacillus amyloliquefaciens WB1 bacterial concentrations are more than or equal to 1 × 1010CFU/mL；
The amount of the marine oligosaccharide class prebioticses for adding accounts for the 5～8% of the bacillus amyloliquefaciens WB1 bacterium solution quality；
The amount of the bacteria protectant for adding accounts for the 5～10% of the bacillus amyloliquefaciens WB1 bacterium solution quality；
The bacteria protectant is defatted milk powder；
The volume mass that mixed liquor described in step S2 is mixed with plant source carrier compares 1:0.25mL/g；
The plant source carrier is maize cob meal.
7. the preparation method of ocean symphysis unit additive according to claim 6, it is characterised in that
The bacillus amyloliquefaciens WB1 bacterial concentrations are equal to 1 × 1010CFU/mL；
The mass concentration of the marine oligosaccharide class prebioticses for adding is the 5% of the bacillus amyloliquefaciens WB1 bacterium solutions；
The mass concentration of the bacteria protectant for adding is the 10% of the bacillus amyloliquefaciens WB1 bacterium solutions.
8. the application of symphysis unit additive in ocean described in claim 1, it is characterised in that add as the feedstuff for apostichopus japonicus culture
Plus agent.
9. the application of ocean symphysis unit additive according to claim 8, it is characterised in that concrete operations are：By the sea
Foreign symphysis unit additive is mixed in proportion with Stichopus japonicuss normal feedstuff；
The ocean symphysis unit additive is the 2～5% of the normal feedstuff quality.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109607811A (en) * | 2018-11-23 | 2019-04-12 | 王熙涛 | A kind of preparation and application of the dedicated micro- raw first preparation of the steady water of apostichopus japonicus culture |
| CN109601446A (en) * | 2018-11-23 | 2019-04-12 | 王熙涛 | A kind of preparation and application of the dedicated micro- raw first preparation of the steady water of prawn culturing |
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