CN105901305A - Antibiotic substitute feed additive, its formula, feed with antibiotic substitute feed additive and production method of feed with antibiotic substitute feed additive - Google Patents
Antibiotic substitute feed additive, its formula, feed with antibiotic substitute feed additive and production method of feed with antibiotic substitute feed additive Download PDFInfo
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Abstract
The invention relates to an antibiotic substitute feed additive, a formula of the antibiotic substitute feed additive, feed with the antibiotic substitute feed additive and a production method of the feed with the antibiotic substitute feed additive. The antibiotic substitute feed additive is a natural antibiotic component. The formula of the antibiotic substitute feed additive comprises a natural antibiotic component, trace elements, a compound enzyme, probiotics, prebiotics and an acid preparation. The invention also relates to the production method of the feed with the antibiotic substitute feed additive.
Description
Invention field
The present invention relates to substitute antibiotics feed addictive, substitute antibiotics feed addictive prescription, the feed that comprises it
And production method.In particular it relates to substitute antibiotics feed addictive is a kind of natural antimicrobial component；This
Bright substitute antibiotics feed addictive prescription comprises natural antimicrobial component, trace element, complex enzyme, probiotic, prebiotics
With acid supplement etc.；The method that the invention still further relates to produce the feed comprising it.
Background of invention
China is the country that animal products yield is maximum in the world, is also the country that antibiotic is applied or abuse is the most serious
One of.Because Yao Can problem China most animals products export amount is the highest, seriously constrain the development of China's poultry industry.Feeding antibiosis
The research application of element substitute is a task the most urgent.
In livestock and poultry cultivation, apply antibiotic, with diseases prevention, raising growth of animals or poultry and feed conversion rate etc., but can have animal
Develop immunity to drugs, the negative effect such as medicament residue and damage to the environment.
It is in livestock and poultry cultivation and applies antibiotic, existing good effect, such as diseases prevention, improves growth and feed conversion rate
Deng, more there is negative effect, such as animal develops immunity to drugs, medicament residue and damage to the environment etc..21 century, countries in the world all limited
Or prohibit the use of antibiotic.Exploitation Substitutes For Antibiotic is extremely urgent, but disables antibiotic completely, and livestock is easy must die of illness
Dying, feed efficiency is the highest, can directly influence the development of livestock and poultry breeding industry, therefore studies, develops Substitutes For Antibiotic and become mesh
The focus of former world livestock-raising technical field research.
Along with society and expanding economy, people are to the demand of nuisanceless animal derived product (meat, egg, milk etc.) day by day
Urgently.The exceeding standard of antibiotic in conventional animal production process, Use overrun makes consumer's edible safety to animal product
Worry.FAO (Food and Agriculture Organization of the United Nation) (FAO) in 2005, International Animal Health tissue (OIE) and the World Health Organization (WHO) carry jointly
Having gone out " resistance beginning " concept to control the use of veterinary medicine, animal is used antibiotic to prevent by following thoroughly forbidding
The significant damage producing the mankind, wherein European Union disabled antibiotics product in 2006 in feed comprehensively.Thus, novel
Safety and environmental protection feed nuisanceless, pollution-free, noresidue becomes the focus of current research exploitation, and safety and environmental protection feed addictive
Exploitation application be its emphasis.
The developing direction of future in feed additive be have concurrently nontoxic, noresidue, have no drug resistance, green free from environmental pollution adds
Add agent.
Along with the development of science and technology, the continuous progress of feed addictive basic research, Substitutes For Antibiotic is studied
Deepen continuously, Substitutes For Antibiotic novel, effective, comprehensive will have broad application prospects.
According to relevant information, the 50% of current whole world antibiotics production total amount is for animal husbandry, and China is existing feeding
The year market demand of antibiotic is about 30,000,000,000 yuans, accordingly, as the safe and efficient biological system of substitute antibiotics
Agent has the good market space.
Along with the development of whole world animal husbandry, the especially rise of large-scale cultivation, whole world feed market yield is fast in recent years
Speed increases, the statistics can issued according to whole world Combined fodder:
Global forage yield in 2011 is up to 873,000,000 tons, and whole world feedstuff industry total industrial output value is 350,000,000,000 dollars.
Feed addictive refers to a small amount of or micro substance added during Feed Manufacturing processing, use, in feed
Seldom but effect is notable for consumption.Feed addictive is the raw material that modern feed industry necessarily uses, to strengthening basal feed nutrition
Being worth, improve breeding performonce fo animals, it is ensured that animal health, save feed cost, improving the aspects such as livestock products quality has significantly
Effect.The development increasing to feed additive industry of feed feed total output both at home and abroad provides good development foundation
At European & American Market, feeding antibiotic is gradually replaced by the substitute of antibiotic, and other parts of the world also have equally
Trend.Replacement is now used antibiotic by the new antibiotic having no side effect；Vegetalitas and natural feed addictive market will have
Bigger growth；The application of the additive being suitable to new feed processing technology will rise appreciably.Feed addictive market, whole world pin
Volume of selling increases to 6,400,000,000 dollars in 2006 from the 6300000000 of calendar year 2001 dollars, and average growth rate per annum is 0.2%.
Although countries in the world livestock and poultry, pharmaceutical science man effort under, developed the life of multiple part substitute antibiotics
Produce product, but practical application effect is unsatisfactory, there is no a kind of product that can replace antibiotic completely so far.
The purpose of the present invention: exploitation substantially can replace antibiotic nontoxic, noresidue, have no drug resistance, free of contamination green
Feed addictive.
Utilize compound combined method, in conjunction with natural antimicrobial component, the Immune-enhancing effect factor, trace element etc., substitute antibiosis
The diseases prevention ability of element, adds complex enzyme, probiotic, prebiotics and acid supplement technology etc., and the growth promoter of substitute antibiotics is made
With, filter out effective product.
The present invention succeeds in developing, and using as a kind of more satisfactory Substitutes For Antibiotic, is improving livestock and poultry cultivation quality
And benefit, development nuisanceless green cultivation, realize increasing peasant income, industry synergy and sustainable development of animal husbandry there is important meaning
Justice.
Summary of the invention
The present invention is on the basis of using for reference existing various product technology, in conjunction with international forward position biotechnology, according to domestic poultry
The reality of fowl cultivation, exploitation team is in the professional speciality in the fields such as molecular biology, pharmacy, chemistry, according to natural anti-
Fungus matter, organic acid, oligosaccharide kind and trace element etc. carry out functional analysis, study its conspiracy relation each other and dose-effect
Relation, and the mechanism of action to animal, develop a function compared with comprehensively, effect becomes apparent from, security is high, harmless without residual
Stay, the composite biological preparation of wide adaptability, to preferably replacing existing antibiotics feedstuff additive product.
The present invention wants the key problem in technology problem that emphasis solves:
(1) on the basis of with reference to existing antibiotic substitute matter and other natural antibiotics materials, break the normal procedure, change
Become the mode of thinking, tentatively carry out compound combination, synergy should be produced；
(2) substitutional relation in feed between this compound product and original antibiotic product is solved；
(3) economy and the adaptability problem of substitute products are solved.
The principle of foundation of the present invention,
1) choose there is the natural component of antibacterial action, directly replace the effect (plant of antibiotic resistance pathogenic microorganism
Extract, natural products).
2) improve animal immunocompetence, strengthen animal self resistance against diseases (natural component).
3) suppress GI pernicious bacteria, strengthen the activity of beneficial flora, promote that animal is towards preferably material meat conversion
(prebiotics, probio, acidulant, enzyme).
4) some compositions can also reduce the content of ammonia in ight soil, makes the living environment of animal improve, and raises place to ring
The air pollution in border reduces (trace element, mineral matter).
Therefore, on the one hand, the present invention provides a kind of substitute antibiotics feed addictive, and it is a kind of natural antibacterial one-tenth
Point.
In the present invention, described natural antimicrobial component is selected from: Echinacea extract, protects brother fruit, coconut oil, wild marjoram
Oil, Olive leaf P.E, Astragalus Root P.E, garlic juice, Andrographis Paniculata, grapefruit seed extract, propolis extract and
Its any combination；It is preferably Astragalus Root P.E, garlic juice, Andrographis Paniculata, propolis extract and any combination thereof；More preferably
For garlic juice, Andrographis Paniculata, propolis extract and any combination thereof；It is further preferably Andrographis Paniculata, propolis extract
And any combination；Most preferably propolis extract.
Another further aspect, the present invention provides a kind of substitute antibiotics feed addictive prescription, and it comprises described in one or more
Natural antimicrobial component.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it also comprises prebiotics.In the present invention
Substitute antibiotics feed addictive prescription in, wherein said prebiotics is oligosaccharide, especially xylo-oligosaccharide, mannose, really
Oligosaccharides, chitosan oligosaccharide and combinations thereof；More preferably less than xylan, chitosan oligosaccharide.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it also comprises trace element, described micro-
Secondary element is selected from zinc (especially zinc sulfate), and selenium (especially sodium selenite, match happy selenium, selenium methionine, yeast selenium), germanium is (especially
It is inorganic oxide germanium, organic germanium 132) and combinations thereof.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it also comprises mineral matter, such as zeolite
Powder (silica), medical stone.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it also comprises the enzyme system selected from following enzyme
Agent: cellulase, 1,4 beta-glucanase, lipase, mannonase pectase, phytase, protease, zytase and group thereof
Close；It is preferably cellulase, pectase, phytase and any combination thereof；Most preferably cellulase, protease.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it also comprises probiotic, described prebiotic
Element is selected from bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, streptococcus fecalis saccharomycete, candida utili, wine brewing ferment
Female and combinations thereof；It is preferably bacillus subtilis, lactobacillus acidophilus, saccharomyces cerevisiae and combinations thereof；More preferably bacillus subtilis,
Lactobacillus acidophilus and combinations thereof.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it also comprises acid compound formulation, described
Acid compound formulation is selected from formic acid, calcium formate, phosphoric acid, fumaric acid, citric acid and trisodium citrate and combinations thereof；It is preferably first
Acid, phosphoric acid, citric acid and combinations thereof.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it comprises organic germanium 132, zeolite powder,
Zinc sulfate.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it comprises yeast, selenium, xylo-oligosaccharide,
Citric acid, organic germanium 132, zeolite powder.
In the substitute antibiotics feed addictive prescription embodiment of the present invention, it comprises xylo-oligosaccharide, zinc (sulfuric acid
Zinc), organic germanium 132, zeolite powder.
The present invention also provides for a kind of feed, and it comprises one or more described natural antimicrobial components described or its bag
Described substitute antibiotics feed addictive prescription containing the present invention.
In the feed embodiment of the present invention, the propolis content of wherein said natural antimicrobial component is that 0.4mg arrives
400mg/kg feed, preferably 1mg to 300mg/kg feed, more preferably 5mg to 200mg/kg feed, further preferably 10mg arrives
120mg/kg feed, most preferably 50mg to 100mg/kg feed.
In the feed embodiment of the present invention, it comprises described natural antimicrobial component.Feed of the present invention comprises conduct
The described Andrographis Paniculata of described natural antimicrobial component, and the content of described Andrographis Paniculata is that 12.5mg arrives
200g/kg feed, preferably 25mg to 150g/kg feed, more preferably 50mg to 100g/kg feed, further preferably 100mg to 50g/
Kg feed, most preferably 150mg to 1g/kg feed.
In the feed embodiment of the present invention, it comprises probiotic.The content of described probiotic is (bacillus subtilis
Bacterium, lactobacillus acidophilus 1: 3) 0.1-10g/kg feed；It is preferably (bacillus subtilis, lactobacillus acidophilus 1: 3) 0.5-5g/kg to raise
Material, more preferably (bacillus subtilis, lactobacillus acidophilus 1: 3) 1-3g/kg feed；Further preferably (bacillus subtilis, addicted to yogurt bar
Bacterium 1: 3) 1-2g/kg feed, most preferably bacillus subtilis, lactobacillus acidophilus 1: 3) 1.2g/kg feed.
In the feed embodiment of the present invention, it comprises Compound-acid preparation.Described Compound-acid preparation (phosphoric acid accounts for 30%,
Citric acid accounts for 20%, fumaric acid accounts for 20%, calcium formate accounts for 5%) content be 0.05-3% (w/w)；It is preferably
0.1-2% (w/w)；More preferably 0.1-1.5% (w/w)；It is further preferably 0.1-1, most preferably 0.2-
0.3% (w/w).
In the feed embodiment of the present invention, it comprises prebiotics.As prebiotics, the xylo-oligosaccharide content that it comprises
For xylo-oligosaccharide 10mg-300mg/kg feed；It is preferably xylo-oligosaccharide 20mg-200mg/kg feed；It it is further preferably xylo-oligosaccharide
50mg-180mg/kg feed, more preferably xylo-oligosaccharide 80mg-150mg/kg feed, minimum xylan 100mg/kg feed.
In the feed embodiment of the present invention, it comprises trace element.As trace element, its organic germanium comprised
132 is organic germanium 132 0.1-100mg/kg feed；It is preferably organic germanium 132 1-50mg/kg feed；More preferably organic germanium 132
10-25mg/kg feed；More preferably organic germanium 132 10-20mg/kg feed；More preferably organic germanium 132 12mg/kg feed.
The inorganic oxide germanium powder that feed of the present invention comprises is 10-20mg/kg feed.
In the feed embodiment of the present invention, it comprises mineral matter.As mineral matter, the zeolite that feed of the present invention comprises
Powder content is zeolite powder 5-100g/kg feed；Preferred zeolite powder 10-80g/kg feed；More preferably zeolite powder 20-40g/kg raises
Material；Further preferably zeolite powder 25-35g/kg feed；More preferably zeolite powder 30g/kg feed.
According to spirit of the invention, feed of the present invention comprises described natural antimicrobial component, the Immune-enhancing effect factor, trace unit
Element, complex enzyme, probiotic, prebiotics and acid supplement technology and any combination thereof.
The described substitute antibiotics feed addictive that the present invention provides, described substitute antibiotics feed addictive prescription are dynamic
Purposes in thing feed.Described substitute antibiotics feed addictive, substitute antibiotics feed addictive prescription and the institute of the present invention
State feed and be applicable to any letting animals feed；Described animal is preferably poultry, domestic animal, fish, pet etc.；Described livestock and poultry example
Pig, chicken, quail, birds, duck class, goose, ox, horse, sheep, dog, snake etc. in this way；Described pet e.g. dog, cat, pig, chicken, duck, goose,
Bird, pig, snake, horse, mouse etc..
The present invention also provides for a kind of method of feed producing the claim present invention, and the method includes: by described replacement
Antibiotic feed additive or described substitute antibiotics feed addictive prescription add in normal diet.
Accompanying drawing explanation
Fig. 1. diagram carrys out the flow chart of the composition of screening formula by the method that external bacterium is tested.Survey used by the flow process left side
Examination natural antimicrobial substance gram-positive bacteria is with gold-coloured staphylococci, and gas capsular clostridium is representative (Gram-positive
Bacteria:Staphylococcus aureus, C.perfringens), Gram-negative bacteria is with Pseudomonas aeruginosa, deformation
Bacillus is representative (Bacillus pyocyaneus, proteus species).Test on the right of flow process and intestinal beneficial flora is had
Bacterium used by the material of facilitation is Bacillus acidi lactici and Bifidobacterium.* the flora selected is also for Bacillus acidi lactici and bifid bar
Bacterium.
Fig. 2. diagram replaces antibiotic feed additive animal testing and flow sheet.
The specific embodiment of the invention
The method of the present invention, technology path and technological process:
(1) existing antibiotic substitute matter and trace element analysis are studied, to other without xicity related, pollution-free natural
Antibacterial material is analyzed and researched；
(2) the biochemistry relation between above-mentioned each material is studied, and effect conspiracy relation, true by the dose-effect relationship of prescription
Fixed three groups of compounds formula；Study the physicochemical character of each group of compound, determine preliminary product formulation；
(3) research compound preparation is to pig, the mechanism of action of chicken；
(4) choose pig, chicken test site, carry out 3 phase feeding experiments by statistics requirement respectively；
(5) compound preparation is observed to pig, the antibacterial protection effect of chicken, growth-promoting effect, meat Egg Quality improved effect.With
Salinomycin Sodium is as a control group；
(6) study this preparation to pig, the economy of fowl raising, make reference with Salinomycin Sodium.Mainly investigate for raiser
The change of input-output ratio；
(7) target level of product quality is researched to developed；
(8) research to develop raise pigs, safe and efficient feed additive technology normative document of raising chickens a set of.
The present invention is natural extract matter or natural fermentation products, the discharge of process of manufacture unharmful substance, for
Environment is without adverse effect.
The method tested with external bacterium is carried out the flow chart (seeing Fig. 1) of the composition of screening formula and is existed according to antibiotic
The mechanism of action of animal feeding and create.
Test natural antimicrobial substance gram-positive bacteria used by the flow process left side with gold-coloured staphylococci, gas capsular clostridium is
Represent (Gram-positive bacteria:Staphylococcus aureus, C.perfringens), Gram-negative bacteria
With Pseudomonas aeruginosa, proteus is representative (Bacillus pyocyaneus, proteus species).
Testing the bacterium used by the material having facilitation to intestinal beneficial flora on the right of flow process is Bacillus acidi lactici and bifid
Bacillus.
* the flora selected is also for Bacillus acidi lactici and Bifidobacterium
Constant broth dilution method:
1. prepare 10 test tubes, in 10 test tubes, add 1ML nutrient broth respectively.
2. adding the mixing of 1ML medicine in first test tube, inhale 1ML and add in second test tube, mixing, the 2nd examination
Pipe is inhaled 1ML and adds the 3rd ..., by that analogy, until the tenth, merely hit the tenth and discard 1ML.
It is separately added into 1ML bacterium solution (10000FU/ML) mixing the most again in 10 test tubes.
4. put into incubator to cultivate 16 to 24 hours, take out observation until which test tube is also clarified, the medicine of which test tube
Concentration is exactly MIC.
Micro-dilution method, operating procedure:
1. antibacterials and culture medium are prepared with constant broth dilution method.
Prepared by 2.MIC plate: sterile working, and the antibacterials solution of variable concentrations after doubling dilution is added separately to sterilizing
In 96 hole polystyrene plates, the 1st to the 11st hole adds liquid, and every hole 10 μ l, the 12nd hole not dosing, as growth control, freezes dried
Rear sealing, less than-20 DEG C save backup.
3. the concentration prepared by growth method or direct bacteria suspension method is equivalent to 0.5 Maxwell than turbid standard by inoculum preparation
Bacteria suspension, after MH meat soup 1: 1000 dilutes, adds 100 μ l in every hole, seals in rearmounted 35 DEG C of normal air incubators, hatch 16
～20h judged result.When test hemophilus, during streptococcus, incubation time is 20～24h.
4. result judges with the lowest concentration of drug completely inhibiting bacterial growth in aperture as MIC.Work as Positive control wells
(i.e. without antibiotic) obvious growth test of interior bacterium is the most meaningful.Generally for gram negative bacilli, trace broth dilution
The MIC that method records is identical with the result that constant broth dilution method records or a low dilution factor.
Replace antibiotic feed additive animal testing and flow sheet, see Fig. 2.
The good technique effect of the present invention:
(1) present invention is on the basis of studying existing antibiotic substitute products and other natural materials, breaks the normal procedure, changes
Become the mode of thinking, tentatively carry out compound combination, produce synergy；Wide, strong adaptability, good economy performance to realize function
Purpose, is the innovation of the present invention；
(2) achievement in research of the present invention can solve the limitation of existing Substitutes For Antibiotic substantially, keeps and strengthens original merit
Can, it is one of the characteristic of the present invention；
(3) achievement in research of the present invention will be security height, noresidue, non-hazardous and pollution, can Long-Time Service, do not produce
The raw resistance to the action of a drug, also without lay-off period, this is also one of the characteristic of the present invention.
Embodiment
Embodiment 1
The preparation method of substitute antibiotics feed addictive prescription of the present invention
The preparation method of substitute antibiotics feed addictive prescription of the present invention comprises the following steps:
Step 1: choose external minimum antibacterial (MIC) low one (A) from natural antibacterial ingredient or two kinds (A, B) have
Synergistic antibacterial one-tenth；
Step 2: testing in vitro screening to the positivity effect of normal intestinal flora or have no adverse effects the factor (a, b, c, d,
E ...), and find out its suitable concentration；
Step 3: result A that step 1 is selected, or A+B adds, less than the concentration of MIC, the immunity increasing that step 2 is selected successively
The strong factor, a, b, c, d, e ...
With 96 hole micro-plate, S. aureus L-forms, lactobacillus acidophilus is test strain, and every kind of combination 4 hole is repeated.
To strengthen S. aureus L-forms bacteriostasis, strain of lactobacillus acidophilus selects formula combination based on growing fine.
The preparation of garlic juice: fresh garlic peeling is cleaned and dries surface moisture, weigh garlic 100mg, with clean family
With pounding garlic device, garlic is pounded mashed garlic (adding sodium chloride 0 9g in advance in pounding garlic device, to reduce the peculiar smell of garlic), 4 000r/
Min is centrifuged 10min and draws supernatant 20ml, filtration sterilization, dispenses bottle, puts-20 DEG C of Refrigerator stores.By garlic juice from ice during experiment
Case takes out, and after thoroughly dissolving, carries out doubling dilution with physiological saline, puts 20,37,56 and 80 DEG C of water-bath 60min respectively.
Find out minimal inhibitory concentration MIC of antimicrobial component:
Aureomycin is 1.5 μ g/ml to staphylococcic minimal inhibitory concentration MIC.
Propolis is 10 μ g/ml to staphylococcic minimal inhibitory concentration MIC.
Andrographis Paniculata is 0.5mg/ml to S. aureus L-forms minimum inhibitory concentration MIC.
The allicin of 25% is 0.1mg/ml to S. aureus L-forms minimum inhibitory concentration MIC.
Garlic liquid is 1: 64 to S. aureus L-forms minimum inhibitory concentration MIC.
Lack synergy concentration
Embodiment 2 antibiotic substitutes feed addictive prescription
The antibiotic that in-vitro screening obtains in aforementioned manners substitutes feed addictive prescription.
Pollution-free antibiotic replacement feed addictive prescription example 1: propolis and germanium substitute antibiotics formula (Propolis&
Germanium replace Antibiotic formula)
Compound method:
2. 20 grams are weighed
A preparation packaging operation flow process (without zeolite powder)
B preparation packaging operation flow process (containing zeolite powder)
2. 20 grams are weighed
Pollution-free antibiotic substitutes feed addictive example #2
1. container:
Stainless-steel pan with cover, (capacity about 2 liters)
2. agitator: stainless steel soup ladle
3. stirring means:
1) by load weighted composition 1.-4., 2. 1/2 amount that the most first takes is inserted in container；
2) repeatedly stirring mixing with agitator, general employing is stirred 30 times clockwise, then stirring 30 times counterclockwise, the most anti-
Multiple 5 times；
3) 1/2 amount that adds repeats said method the most afterwards；
4) again by rotating each 30 times of mixing along counter-clockwise mode after adding a cover, 3 times the most repeatedly.
Pollution-free antibiotic substitutes feed addictive prescription example #3
(Galic extract and probiotics replace Antibiotic formula)
Substitute antibiotics feed addictive prescription example 4
Compound method
1. container:
Stainless-steel pan with cover, (capacity about 2 liters)
2. agitator: stainless steel soup ladle
3. stirring means:
1) by load weighted composition 1.-4. insert in container；2) repeatedly stirring mixing with agitator, general employing is clockwise
Stir 30 times, then stirring 30 times counterclockwise, 5 times the most repeatedly；
3 add a cover after again by rotating each 30 times of mixing along counter-clockwise mode, the most repeatedly 3 times altogether.
The prebiotic factor can be added with a kind of antimicrobial component in micro-plate compound with multiple, measure it to Bacillus acidi lactici
Growth effect, with growing way good for coordinating basic point, then the composition that can not be able to add plus micro-plate is (such as microorganism, zeolite powder
Deng).
Key property:
1. the balance combination of selected compounds
2. combined hearth-care unit causes improving palatability, growth of animal performance and feed dietary intake conversion ratio with acidulant.
The disease that the most pre-anti-diarrhea is relevant with enteron aisle.Heat-killed lactic acid bacteria and cell fragment thereof adhere to gut epithelium
Cell causes biomembranous formation and the adhesion stoping enteric pathogenic bacteria and invasion.Bacteriocin class material kills and suppresses pathogen
As Escherichia coli, salmonella and staphylococcus aureus increase.
The most immune regulation: Herba Andrographitis perfume (or spice) extracts antibacterial activity thing and immunomodulatory agents, promotes the siberian crabapple of animal
System.
Embodiment 3
The experiment effect of substitute antibiotics feed addictive prescription of the present invention
Experimental design:
1* such as situation does not allows, and negative control can be removed from.
Materials and methods
1.1 animals used as test and packet
Choosing the weanling pig 72 of health, close by body weight, the principle of male and female half and half is divided into 4 process groups, each place
Reason group 3 repetition, 6 piglets of each repetition.Each group original body mass no significant difference, average weight is 8.0kg.
1.2 experimental diets and experimental design
Experimental group formula 1 or 2 (A or B) organize produced above.The basal diet of experiment is with reference to NRC (1998) 8-20Kg body
Trophic level preparation [5] of weight piglet, is provided by Wen Shi group great Hua agriculture Animal Health Care Products Corporation.
Test and process 3 repetition Random Design experiments according to single factor test 4, test from the beginning of 28 age in days wean, 28d (4 by a definite date
Week)；Experiment point 4 groups is carried out: respectively group basal diet is identical, and the 1st group is control group, without, the 2nd group is added conventional antibiosis
Element, 3 to the 4th groups are added A or B formula in the basal diet of weanling pig respectively.
1.3 feeding and management
This experiment is carried out on Wen Shi group scientific research pig farm.Experiment feeding piglet is in closing, half slatted floor formula pig house, each
Repeat feeding piglet in a hurdle, free choice feeding, drinking-water.Raise according to routine and immune programme for children carry out feeding and management.
1.4 testing indexs and method
1) every pig daily inleting appetite is recorded.
2) every pig body weight growth pattern is recorded
3) record eats health condition and the disease event between period and whole growth cycle with group pig, and it is smelly to compare pigsty
Taste, daily ration intake.Test last day, weigh the weight of every pig.
Experimental result
Effects of green fodder on growth performance of weaned piglet
The impact that weaned piglets is grown by green feed additive
Ss be variance analysis group between sampling error；Sxa-xb is the difference standard error of Newman-Kouls inspection.
Comparative result two-by-two between Newman-Keuls test process group
As can be seen that formula 1 compares with antibiotic with formula 2, have non-at each time point than non-interpolation control group
The most significantly effect of gain, at first 14 days raised, without significant difference between two formulas of antibiotic set of applications and experiment.
Two weeks after antibiotic and our formula between the difference of statistical significance occurs.And to wean between our two formulas
The effect of gain indifference of piggy.
Food consumption and material meat conversion ratio
Comparative formula 2, fills a prescription 1, although and salinomycin weightening finish seen from salinomycin material meat conversion ratio more preferably, but the profit to feed
With being already clearly below our green formula.Illustrate to add in diet the feed addictive that we choose can improve daily gain and
Feed conversion rate, and feed conversion rate effect is better than salinomycin.
The compound confirming us of first in vivo studies is effective, has both improved and has improve weightening finish growth (P ＜ 0.01),
Also promote material meat conversion more effectively (P ＜ 0.01).
Entrance culture experiment:
The performance indications of product are as follows:
1. culture experiment protection effect ≈ aureomycin hydrochloride (not less than 80%)
2. culture experiment feed conversion rate ≈ aureomycin hydrochloride (not less than 80%)
It is below that pollution-free antibiotic replacement feed addictive #1 sums up at the raising experiment of 3900 chickens:
A group: antibiotic substitutes feed addictive #1, without zeolite powder
B group: antibiotic substitutes feed addictive #1, containing zeolite powder
Control group is Conventional antibiotic (15% aureomycin 50mg/kg) application comparison
*
**
* bursa fabricic
The death that * has is unrelated with bursa fabricic
A, B two groups is that different antibiotic substitutes compound, and control group is Conventional antibiotic (15% aureomycin 50mg/kg)
Application comparison.B compound shows disease-resistant different than compareing all significances with weightening finish.
Statistical analysis shows B experimental group difference (U inspection, P ＜ than antibiotic control group significance
0.001), the death rate of animal also has dramatically different (X2 checks P ＜ 0.005) than antibiotic control group). this result shows B
Formula is effective in body weight and the prevention disease of increase animal subject.
Cause by infectious bursa diseases virus that the one of 2-6 week old chick is acute, high degree in contact sexually transmitted disease.With Fa Shi
Tumour is swollen, hemorrhage, downright bad, chest muscle, leg flesh are hemorrhage, and glandular stomach, muscular stomach intersection strip is hemorrhage is characterized.Aureomycin is to this virus nothing
Effect.
Test sample uses when being produced on broiler breeding and performs by S.O.P. (SOP), carries out sucking pig and tests other
Animal experiment sample is formulated with mark and can follow.It is proved a group of formula actually active, so this compound is substituting based on broiler trial
Antibiotic is initial success, more ripe.The in vivo studies of other species can be carried out with similarity method.
Bibliography:
John Wallace Plants and Plant Extracts to Replace Growth-Promoting
Antibiotics in Farm Livestock Production Published on 1 September 2010 in
Sustainability and Communities, Food, health and wellbeing
Jacela, J.Y.；DeRouchey, Joel M.；Tokach, Michael D.；Goodband, Robert D.；
Nelssen, Jim L.；Renter, David G.；Dritz, Steven S.Feed additives for swine:Fact
Sheets-prebiotics and probiotics, and phytogenics Journal of Swine Health and
Production, Volume:18, Issue:3, Starting Page:132, Ending Page:136
J.D.Richards, J.Gong, C.F.M.de LangeThe gastrointestinal microbiota and
Its role in monogastric nutrition and health with an emphasis on pigs:Current
Understanding, possible modulations, and new technologies for ecological
Studies Canadian Journal of Animal Science, 2005,85 (4): 421-435,10.4141/A05-049
Vonne Lund, Bo Algers Research on animal health and welfare in organic
farming
Claims (9)
1. a substitute antibiotics feed addictive, it is a kind of natural antimicrobial component, wherein said natural antimicrobial component
It is selected from: Echinacea extract, protects brother fruit, coconut oil, origanum oil, Olive leaf P.E, Astragalus Root P.E, garlic juice, punching
Lotus extract, grapefruit seed extract, propolis extract and any combination thereof；It is preferably Astragalus Root P.E, garlic juice, punching
Lotus extract, propolis extract and any combination thereof；More preferably garlic juice, Andrographis Paniculata, propolis extract and appointing
Meaning combination；It is further preferably Andrographis Paniculata, propolis extract and any combination thereof；Most preferably propolis extract.
2. a substitute antibiotics feed addictive prescription, it comprises the natural antimicrobial component of claim 1.
3. the substitute antibiotics feed addictive prescription of claim 2, it also comprises prebiotics, and the most described prebiotics is oligomerization
Sugar, especially xylo-oligosaccharide, mannose, FOS, chitosan oligosaccharide and combinations thereof；More preferably less than xylan, chitosan oligosaccharide；And/or
Being optional that, it also comprises trace element, and described trace element is selected from zinc (especially zinc sulfate), selenium (especially sub-selenium
Acid sodium, match happy selenium, selenium methionine, yeast selenium), germanium (especially inorganic oxide germanium, organic germanium 132) and combinations thereof；And/or
Being optional that, it also comprises mineral matter, such as zeolite powder (silica), medical stone；And/or
Being optional that, it also comprises the enzyme preparation selected from following enzyme: cellulase, 1,4 beta-glucanase, lipase, mannosan
Enzyme, pectase, phytase, protease, zytase and combinations thereof；It is preferably cellulase, pectase, phytase and arbitrarily
Combination；Most preferably cellulase, protease；And/or
Being optional that, it also comprises probiotic, and described probiotic is selected from bacillus subtilis, and bacillus licheniformis, addicted to yogurt bar
Bacterium, streptococcus fecalis saccharomycete, candida utili, saccharomyces cerevisiae and combinations thereof；It is preferably bacillus subtilis, addicted to yogurt bar
Bacterium, saccharomyces cerevisiae and combinations thereof；More preferably bacillus subtilis, lactobacillus acidophilus and combinations thereof；And/or
Be optional that, its also comprise acid compound formulation, described acid compound formulation selected from formic acid, calcium formate, phosphoric acid, fumaric acid,
Citric acid and trisodium citrate and combinations thereof；It is preferably formic acid, phosphoric acid, citric acid and combinations thereof.
4. a feed, its substitute antibiotics feed addictive comprising claim 1 or any one of claim 2-3
Substitute antibiotics feed addictive prescription.
5. the feed of claim 4, the propolis content of wherein said natural antimicrobial component is 0.4mg to 400mg/kg feed,
It is preferably 1mg to 300mg/kg feed, more preferably 5mg to 200mg/kg feed, further preferably 10mg to 120mg/kg feed, optimum
Elect 50mg to 100mg/kg feed as.
6. the feed of claim 4, it comprises the Andrographis Paniculata as described natural antimicrobial component, and described punching
The content of lotus extract is 12.5mg to 200g/kg feed, preferably 25mg to 150g/kg feed, more preferably 50mg to 100g/
Kg feed, further preferably 100mg to 50g/kg feed, most preferably 150mg to 1g/kg feed.
7. the feed of any one of claim 4-6, the content of its probiotic is (bacillus subtilis, lactobacillus acidophilus 1: 3)
0.1-10g/kg feed；It is preferably (bacillus subtilis, lactobacillus acidophilus 1: 3) 0.5-5g/kg feed, more preferably (withered grass bud
Spore bacillus, lactobacillus acidophilus 1: 3) 1-3g/kg feed；Further preferably (bacillus subtilis, lactobacillus acidophilus 1: 3) 1-2g/kg raises
Material, the most further preferably bacillus subtilis, lactobacillus acidophilus 1: 3) 1.2g/kg feed；And/or
It is optional that, its Compound-acid preparation (phosphoric acid accounts for 30%, citric acid accounts for 20%, fumaric acid accounts for 20%, calcium formate accounts for 5%)
Content be 0.05-3% (w/w)；It is preferably 0.1-2% (w/w)；More preferably 0.1-1.5% (weight/
Weight)；Compound-acid preparation (phosphoric acid accounts for 30%, citric acid accounts for 20%, fumaric acid accounts for 20%, calcium formate accounts for 5%) 1% (weight/
Weight)；And/or
Being optional that, the xylo-oligosaccharide content that it comprises is xylo-oligosaccharide 10mg-300mg/kg feed；Xylo-oligosaccharide 20mg-
200mg/kg feed；Xylo-oligosaccharide 50mg-180mg/kg feed, xylo-oligosaccharide 80mg-150mg/kg feed, xylo-oligosaccharide
100mg/kg feed；And/or
Being optional that, the organic germanium 132 that it comprises is organic germanium 1320.1-100mg/kg feed；It is preferably organic germanium 132 1-
50mg/kg feed；More preferably organic germanium 132 10-25mg/kg feed；More preferably organic germanium 132 10-20mg/kg feed；More
Preferably organic germanium 132 12mg/kg feed；And/or
Being optional that, the zeolite powder content that it comprises is zeolite powder 5-100g/kg feed；Preferred zeolite powder 10-80g/kg feed；
More preferably zeolite powder 20-40g/kg feed；Further preferably zeolite powder 25-35g/kg feed；More preferably zeolite powder 30g/kg feed；
And/or
Being optional that, the inorganic oxide germanium powder that it comprises is 10-20mg/kg feed.
8. the substitute antibiotics feed addictive of claim 1, the substitute antibiotics feed addictive prescription of any one of 2-3,4-7
The feed of any one, it is used for any letting animals feed；Described animal is preferably poultry, fish, pet etc.；Described poultry is e.g.
Pig, ox, horse, sheep, dog, snake etc.；Described pet e.g. dog, cat, bird, pig, snake, horse, mouse etc..
9. the method producing the feed of any one of claim 4-7, the method includes: by the replacement antibiosis of claim 1
The substitute antibiotics feed addictive prescription of element feed addictive or any one of claim 2-3 adds in normal diet.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410212229 | 2014-05-19 | ||
| CN2014102122291 | 2014-05-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105901305A true CN105901305A (en) | 2016-08-31 |
Family
ID=56743943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510253266.1A Pending CN105901305A (en) | 2014-05-19 | 2015-05-18 | Antibiotic substitute feed additive, its formula, feed with antibiotic substitute feed additive and production method of feed with antibiotic substitute feed additive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105901305A (en) |
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| CN106562020A (en) * | 2016-10-25 | 2017-04-19 | 界首市洪理养殖专业合作社 | Feed capable of increasing the slaughter rate of Huaihai goats |
| CN106578731A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Marine synbiotics additive and application thereof in stichopus japonicus culture |
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| CN109287874A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For promoting the additive and its preparation method and application of ruminant cub growth and development |
| CN111165676A (en) * | 2020-01-13 | 2020-05-19 | 青岛博隆实验动物有限公司 | Additive for colostrum substitute of puppy, preparation method and application |
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| ES2797999A1 (en) * | 2019-06-04 | 2020-12-04 | Tores Alberto Alejandro Garcia | PREBIOTIC COMPOSITION FOR THE PREVENTIVE TREATMENT OF INTESTINAL INFECTIONS IN CATTLE (Machine-translation by Google Translate, not legally binding) |
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| CN106562020A (en) * | 2016-10-25 | 2017-04-19 | 界首市洪理养殖专业合作社 | Feed capable of increasing the slaughter rate of Huaihai goats |
| CN107047975A (en) * | 2016-12-19 | 2017-08-18 | 广东工业大学 | A kind of composition and feed addictive and preparation method thereof |
| CN106578731A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Marine synbiotics additive and application thereof in stichopus japonicus culture |
| CN106721653A (en) * | 2016-12-20 | 2017-05-31 | 大连赛姆生物工程技术有限公司 | A kind of apostichopus japonicus culture compound symphysis unit additive and its application |
| CN106721654A (en) * | 2016-12-20 | 2017-05-31 | 大连赛姆生物工程技术有限公司 | A kind of apostichopus japonicus culture preparation and its application of symphysis unit additive |
| CN106922972A (en) * | 2017-03-24 | 2017-07-07 | 广州广牧丰生物技术有限公司 | A kind of Efficient antibacterial feedstuff additive product |
| CN106819495A (en) * | 2017-04-13 | 2017-06-13 | 湖南赛福资源饲料科技有限公司 | Standby boar feed and preparation method thereof |
| CN107080068A (en) * | 2017-06-21 | 2017-08-22 | 甘肃农业大学 | A kind of agent for rearing and fattening meat sheep feed and its application |
| CN107484879A (en) * | 2017-08-31 | 2017-12-19 | 深圳市金新农科技股份有限公司 | Feed addictive, antibiotic-free feed and its application |
| CN107821753A (en) * | 2017-11-20 | 2018-03-23 | 江苏雅博动物健康科技有限责任公司 | A kind of pet probiotic granulate preparation and preparation method thereof |
| CN107897514A (en) * | 2017-12-27 | 2018-04-13 | 江南大学 | A kind of compound antimicrobial-free feed additive |
| CN108157606A (en) * | 2018-01-18 | 2018-06-15 | 深圳威普爱客智能科技有限公司 | A kind of feed addictive for promoting growth |
| CN108497200A (en) * | 2018-03-21 | 2018-09-07 | 南京东晨鸽业有限公司 | A kind of nutrient fodder and its preparation method and application |
| CN109287874A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For promoting the additive and its preparation method and application of ruminant cub growth and development |
| ES2797999A1 (en) * | 2019-06-04 | 2020-12-04 | Tores Alberto Alejandro Garcia | PREBIOTIC COMPOSITION FOR THE PREVENTIVE TREATMENT OF INTESTINAL INFECTIONS IN CATTLE (Machine-translation by Google Translate, not legally binding) |
| WO2020245479A1 (en) * | 2019-06-04 | 2020-12-10 | Garcia Tores Alberto Alejandro | Prebiotic composition for the preventative treatment of intestinal infections in livestock |
| CN111165676A (en) * | 2020-01-13 | 2020-05-19 | 青岛博隆实验动物有限公司 | Additive for colostrum substitute of puppy, preparation method and application |
| CN111345402A (en) * | 2020-03-25 | 2020-06-30 | 广州市优百特饲料科技有限公司 | Functional additive and preparation method and application thereof |
| CN113068773A (en) * | 2021-04-14 | 2021-07-06 | 武汉新华扬生物股份有限公司 | Feed additive for replacing antibiotics and preparation method thereof |
| CN113907187A (en) * | 2021-11-01 | 2022-01-11 | 山东省烟台市农业科学研究院 | Compound microbial feed additive and preparation method thereof |
| CN116831210A (en) * | 2023-08-14 | 2023-10-03 | 广州市杰普饲料生物科技有限公司 | Composite biological feed and method for improving activity of probiotics in composite biological feed |
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Application publication date: 20160831 |