CN116831210A - Composite biological feed and method for improving activity of probiotics in composite biological feed - Google Patents
Composite biological feed and method for improving activity of probiotics in composite biological feed Download PDFInfo
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- CN116831210A CN116831210A CN202311026099.8A CN202311026099A CN116831210A CN 116831210 A CN116831210 A CN 116831210A CN 202311026099 A CN202311026099 A CN 202311026099A CN 116831210 A CN116831210 A CN 116831210A
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- biological feed
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- probiotics
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- 239000006041 probiotic Substances 0.000 title claims abstract description 29
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000002131 composite material Substances 0.000 title claims description 15
- 230000000694 effects Effects 0.000 title abstract description 33
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 claims abstract description 64
- 150000001875 compounds Chemical class 0.000 claims abstract description 48
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims abstract description 36
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims abstract description 33
- 235000019691 monocalcium phosphate Nutrition 0.000 claims abstract description 33
- 235000004515 gallic acid Nutrition 0.000 claims abstract description 32
- 229940074391 gallic acid Drugs 0.000 claims abstract description 32
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 28
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 28
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 20
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 19
- 235000016709 nutrition Nutrition 0.000 claims abstract description 16
- 241000186660 Lactobacillus Species 0.000 claims abstract description 13
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 13
- 239000001913 cellulose Substances 0.000 claims abstract description 8
- 229920002678 cellulose Polymers 0.000 claims abstract description 8
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 16
- 241000235342 Saccharomycetes Species 0.000 claims description 15
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 13
- 229940088594 vitamin Drugs 0.000 claims description 13
- 229930003231 vitamin Natural products 0.000 claims description 13
- 235000013343 vitamin Nutrition 0.000 claims description 13
- 239000011782 vitamin Substances 0.000 claims description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 claims description 8
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 8
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 8
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 7
- 244000105624 Arachis hypogaea Species 0.000 claims description 7
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 7
- 235000018262 Arachis monticola Nutrition 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 7
- 235000020232 peanut Nutrition 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 239000004310 lactic acid Substances 0.000 claims description 6
- 235000014655 lactic acid Nutrition 0.000 claims description 6
- 239000010902 straw Substances 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 235000019764 Soybean Meal Nutrition 0.000 claims description 5
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 5
- 239000004455 soybean meal Substances 0.000 claims description 5
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 claims description 5
- 235000005282 vitamin D3 Nutrition 0.000 claims description 5
- 239000011647 vitamin D3 Substances 0.000 claims description 5
- 229940021056 vitamin d3 Drugs 0.000 claims description 5
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 4
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 claims description 4
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 4
- 229930003451 Vitamin B1 Natural products 0.000 claims description 4
- 229930003779 Vitamin B12 Natural products 0.000 claims description 4
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims description 4
- ZAKOWWREFLAJOT-UHFFFAOYSA-N d-alpha-Tocopheryl acetate Natural products CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-UHFFFAOYSA-N 0.000 claims description 4
- 229960000304 folic acid Drugs 0.000 claims description 4
- 235000019152 folic acid Nutrition 0.000 claims description 4
- 239000011724 folic acid Substances 0.000 claims description 4
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 claims description 4
- 229960000342 retinol acetate Drugs 0.000 claims description 4
- 235000019173 retinyl acetate Nutrition 0.000 claims description 4
- QGNJRVVDBSJHIZ-QHLGVNSISA-N retinyl acetate Chemical compound CC(=O)OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C QGNJRVVDBSJHIZ-QHLGVNSISA-N 0.000 claims description 4
- 239000011770 retinyl acetate Substances 0.000 claims description 4
- 235000019192 riboflavin Nutrition 0.000 claims description 4
- 239000002151 riboflavin Substances 0.000 claims description 4
- 229960002477 riboflavin Drugs 0.000 claims description 4
- 229960003495 thiamine Drugs 0.000 claims description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 4
- 229940042585 tocopherol acetate Drugs 0.000 claims description 4
- 235000010374 vitamin B1 Nutrition 0.000 claims description 4
- 239000011691 vitamin B1 Substances 0.000 claims description 4
- 235000019163 vitamin B12 Nutrition 0.000 claims description 4
- 239000011715 vitamin B12 Substances 0.000 claims description 4
- 235000012711 vitamin K3 Nutrition 0.000 claims description 4
- 239000011652 vitamin K3 Substances 0.000 claims description 4
- 230000035899 viability Effects 0.000 claims description 2
- 239000001506 calcium phosphate Substances 0.000 abstract description 17
- 229910000150 monocalcium phosphate Inorganic materials 0.000 abstract description 17
- 206010012735 Diarrhoea Diseases 0.000 abstract description 12
- 244000144977 poultry Species 0.000 abstract description 12
- 230000004083 survival effect Effects 0.000 abstract description 10
- 241001465754 Metazoa Species 0.000 abstract description 6
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- 102000004169 proteins and genes Human genes 0.000 abstract description 4
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- 238000012360 testing method Methods 0.000 description 10
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- 238000002360 preparation method Methods 0.000 description 7
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- 239000000126 substance Substances 0.000 description 6
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- 230000000813 microbial effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
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- 238000009395 breeding Methods 0.000 description 1
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- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
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- 238000000576 coating method Methods 0.000 description 1
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- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000019722 synbiotics Nutrition 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Fodder In General (AREA)
Abstract
The application discloses a compound biological feed and a method for improving the activity of probiotics in the compound biological feed; according to the application, active general probiotics such as bacillus subtilis, streptococcus faecalis, bifidobacteria and lactobacillus are screened, xylooligosaccharide, monocalcium phosphate and gallic acid are adopted, and the survival rate and activity of the probiotics can be improved through the combined action of the xylooligosaccharide, the monocalcium phosphate and the gallic acid, so that the digestion and absorption of the animal on nutritional compositions such as protein, cellulose and the like in the feed are promoted, the diarrhea incidence rate of poultry is reduced, and the feed return is improved.
Description
Technical Field
The application relates to the technical field of feeds, in particular to a compound biological feed and a method for improving the activity of probiotics in the compound biological feed.
Background
The feed is an indispensable key substance for realizing complete nutrition of animals, is a necessary substance for ensuring normal growth and development of livestock and poultry, is widely used in livestock and poultry and aquaculture, is quite common in adding feed additives into the feed, and plays a key role in preventing animal diseases (such as diarrhea), promoting animal growth (weight) and the like.
At present, more products used in the livestock breeding industry are probiotics, synbiotics and the like, but are influenced by the common addition of antibiotics in the existing feed products, probiotics are difficult to stably survive in the feed, the technology of probiotic preparation which is tolerant to feed processing and long-term in vitro preservation is not too closed, the activity is poor, and the application effect is not ideal. Although some microecologics adopt the technology of microcapsule coating with equal height, the survival rate and activity of beneficial bacteria, especially anaerobic probiotics, are not ideal, and the preservation time is short.
The conventional common feed has the defects of poor absorption effect and insignificant weight gain effect.
Disclosure of Invention
The application aims to overcome the defects of the prior art and provides a compound biological feed and a method for improving the activity of probiotics in the compound biological feed.
In order to solve the problems, the application provides the following technical scheme:
in a first aspect, the present application provides a compound biological feed, wherein, per 1000g of the compound biological feed, the compound biological feed comprises:
0.1 to 2g of bacillus subtilis,
0.1 to 2g of streptococcus faecalis,
0.2 to 0.8g of bifidobacterium,
0.4 to 1.2g of lactic acid bacteria,
0.4 to 1.2g of saccharomycete,
0.1 to 0.6g of xylo-oligosaccharide,
10 to 20g of calcium dihydrogen phosphate,
gallic acid 0.4-1.2 g.
As a preferable technical scheme of the application, each 1000g of the compound biological feed comprises the following components:
0.5 to 2g of bacillus subtilis,
0.2 to 1.6g of streptococcus faecalis,
0.3 to 0.8g of bifidobacterium,
0.4 to 0.8g of lactic acid bacteria,
0.4 to 0.8g of saccharomycete,
0.2 to 0.5g of xylo-oligosaccharide,
12-18 g of calcium dihydrogen phosphate,
gallic acid 0.5-1 g.
As a preferable technical scheme of the application, each 1000g of the compound biological feed further comprises:
400-500 g of water.
As a preferred technical solution of the present application, the compound biological feed further comprises a nutritional composition;
the nutritional composition is one or more selected from corn, soybean meal, wheat bran, peanut seedling powder and straw powder.
As a preferable technical scheme of the application, the compound biological feed also comprises vitamins;
the vitamins are one or more selected from vitamin B1, riboflavin, vitamin B6 hydrochloride, folic acid, vitamin K3, vitamin B12, vitamin A acetate, vitamin D3, vitamin E acetate and medium cellulose.
In a second aspect, the present application provides a method of increasing the viability of a probiotic in a compound biological feed, the method comprising:
adding a combination of bacillus subtilis, streptococcus faecalis, bifidobacteria, lactobacillus, saccharomycetes, xylo-oligosaccharide and calcium dihydrogen phosphate into a compound biological feed;
wherein, every 1000g of the composite biological feed comprises the following components in percentage by weight:
0.1 to 2g of bacillus subtilis, 0.1 to 2g of streptococcus faecalis, 0.2 to 0.8g of bifidobacterium, 0.4 to 1.2g of lactobacillus, 0.4 to 1.2g of saccharomycete, 0.1 to 0.6g of xylo-oligosaccharide, 10 to 20g of calcium dihydrogen phosphate and 0.4 to 1.2g of gallic acid.
Compared with the prior art, the application has the following beneficial effects:
according to the application, active general probiotics such as bacillus subtilis, streptococcus faecalis, bifidobacteria and lactobacillus are screened, xylooligosaccharide, monocalcium phosphate and gallic acid are adopted, and the survival rate and activity of the probiotics can be improved through the combined action of the xylooligosaccharide, the monocalcium phosphate and the gallic acid, so that the digestion and absorption of the animal on nutritional compositions such as protein, cellulose and the like in the feed are promoted, the diarrhea incidence rate of poultry is reduced, and the feed return is improved.
Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.
Drawings
FIG. 1 is a schematic diagram of the preparation process of the compound biological feed of the application.
Detailed Description
For a more complete understanding of the present application, reference should be made to the following descriptions and illustrations of the present application in conjunction with the accompanying drawings and the specific embodiments thereof; it will be apparent that the described embodiments are only some, but not all, embodiments of the application; all other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to fall within the scope of the application.
The features, advantages and advantages of the present application will become apparent to those skilled in the art from a reading of the present disclosure.
All percentages, fractions and ratios are by weight of the total composition of the present application, unless otherwise specified. The term "weight content" is used herein to denote the symbol "%".
The terms "comprising," "including," "containing," "having," or other variations thereof herein are intended to cover a non-closed inclusion, without distinguishing between them. The term "comprising" means that other steps and ingredients may be added that do not affect the end result. The term "comprising" also includes the terms "consisting of and" consisting essentially of. The compositions and methods/processes of the present application can comprise, consist of, and consist essentially of the essential elements and limitations described herein, as well as additional or optional ingredients, components, steps, or limitations of any of the embodiments described herein.
In the following schemes, the corresponding starting materials are either commercially available or prepared according to common general knowledge known in the art.
The raw materials in the compound biological feed of the application are all commercially available.
The composite biological feed of the present application is described in detail below.
Composite biological feed
The compound biological feed comprises bacillus subtilis, streptococcus faecalis, bifidobacteria, lactobacillus, saccharomycetes, xylo-oligosaccharide, calcium dihydrogen phosphate and gallic acid.
Wherein bacillus subtilis, streptococcus faecalis, bifidobacterium, lactobacillus and saccharomycetes are probiotics.
The amount of probiotics used in the present application is not particularly limited.
In the application, bacillus subtilis is purchased from Jinan Qinghai chemical industry Co., ltd, streptococcus faecalis is purchased from Wuhan Hua Xiangke biological technology Co., ltd, bifidobacterium is purchased from Jiayi biological engineering Co., shandong, and lactobacillus and microzyme are purchased from Cangzhou Huayu biological technology Co., ltd.
Those skilled in the art know that probiotics are difficult to stably survive in feed, and can have poor vitality and unsatisfactory application effect.
The inventor finds through repeated verification that the xylooligosaccharide, the monocalcium phosphate and the gallic acid are added into the compound biological feed, so that the survival rate and the activity of probiotics can be improved, the digestion and the absorption of organisms on nutrient substances such as feed proteins, cellulose and the like are promoted, and the feed return is improved. The principle of the method is as follows:
the xylooligosaccharide can selectively promote the proliferation of probiotics such as bifidobacteria, bacillus subtilis and the like; the gallic acid can improve the oxidation resistance of the feed, can remove excessive active oxygen substances generated in the feed and organisms, maintains the balance of an oxidation-reduction system, has obvious inhibition effect on pathogenic bacteria after entering the intestinal tract, can improve the intestinal tract morphology and regulate the microbial diversity; in the presence of gallic acid, a more suitable environment can be created, so that more xylo-oligosaccharide participates in the proliferation of probiotics, the capability of the xylo-oligosaccharide for promoting the proliferation of the probiotics is improved, the digestion and absorption capability of animals on nutrient substances is enhanced, and the immunity of organisms is enhanced. The monocalcium phosphate can not only provide mineral nutrition such as phosphorus and calcium, but also increase the ionic strength, and in the application, the monocalcium phosphate can also act synergistically with xylo-oligosaccharide as an improver, so that the proliferation, activity and survival rate of probiotics are improved, the incidence of diarrhea of poultry is reduced, and the growth and development of the livestock are accelerated.
In the present application, both xylo-oligosaccharide and monocalcium phosphate were purchased from Yan biosciences, inc., guangzhou, and gallic acid was purchased from Hubei one-way biosciences, inc.
In a preferred embodiment, the present application provides a compound biological feed, comprising, per 1000 g:
0.1 to 2g of bacillus subtilis,
0.1 to 2g of streptococcus faecalis,
0.2 to 0.8g of bifidobacterium,
0.4 to 1.2g of lactic acid bacteria,
0.4 to 1.2g of saccharomycete,
0.1 to 0.6g of xylo-oligosaccharide,
10 to 20g of calcium dihydrogen phosphate,
gallic acid 0.4-1.2 g.
As a more preferable technical scheme of the application, each 1000g of the compound biological feed comprises the following components:
0.5 to 2g of bacillus subtilis,
0.2 to 1.6g of streptococcus faecalis,
0.3 to 0.8g of bifidobacterium,
0.4 to 0.8g of lactic acid bacteria,
0.4 to 0.8g of saccharomycete,
0.2 to 0.5g of xylo-oligosaccharide,
12-18 g of calcium dihydrogen phosphate,
gallic acid 0.5-1 g.
Among them, the content of monocalcium phosphate is preferably 15 to 17g, more preferably 15.7 to 16.2g.
Water and its preparation method
In the application, each 1000g of the composite biological feed further comprises: 400-500 g of water. The inventor verifies that the water content is lower than 40% and is easy to be underfermented, and the water content is higher than 50% and is easy to be overfermented. Therefore, the present application limits the water content to 40 to 50%.
Namely, the composite biological feed is wet.
Other components
The compound biological feed of the present application may reasonably employ conventional components such as a nutritional composition and vitamins in addition to the above-listed various components within a range that does not significantly impair the effects of the present application.
The kind of the nutritional composition is not particularly limited in the present application, and preferably the nutritional composition is selected from one or more of corn, soybean meal, wheat bran, peanut seedling powder, and straw powder.
For example: every 1000g of the composite biological feed comprises 300-400 g of corn, 50-100 g of bean pulp, 50-100 g of wheat bran, 20-40 g of peanut seedling powder and 20-50 g of straw powder.
More specifically, per 1000g of the compound biological feed:
corn content includes, but is not limited to: 300g, 310g, 320g, 330g, 340g, 350g, 360g, 370g, 380g, 390g, 400g;
the content of the soybean meal includes, but is not limited to: 50g, 60g, 70g, 80g, 85g, 88g, 90g, 95g, 100g;
the content of wheat bran includes, but is not limited to: 50g, 60g, 70g, 80g, 85g, 88g, 90g, 95g, 100g;
the content of peanut seedling powder includes but is not limited to: 20g, 25g, 30g, 32g, 35g, 38g, 40g;
the content of straw powder includes, but is not limited to: 20g, 25g, 30g, 32g, 35g, 38g, 40g, 50g.
In the application, the varieties fed by the composite biological feed comprise but are not limited to chickens, ducks, geese, pigs, cattle and sheep; the specific dosage of the nutritional composition can also be adaptively adjusted for varieties with different growth periods and body types.
For example, when the raised variety is geese, the content of peanut seedling powder can be adjusted to 150-200 g per 1000g of the composite biological feed.
Also, the kind of the vitamins is not particularly limited in the present application, and preferably, the vitamins are selected from one or more of vitamin B1, riboflavin, vitamin B6 hydrochloride, folic acid, vitamin K3, vitamin B12, vitamin a acetate, vitamin D3, vitamin E acetate, and medium cellulose.
For example: every 1000g of the compound biological feed comprises 20-60 g of vitamins.
More preferably, the vitamin is 30g vitamin D3.
The compound biological feed of the application can be prepared by a preparation method known in the art. For example, in connection with fig. 1:
mixing and fermenting bacillus subtilis, streptococcus faecalis, bifidobacterium, lactobacillus, saccharomycetes, xylo-oligosaccharide, monocalcium phosphate and gallic acid in water to obtain fermentation liquor;
the nutrient composition is crushed and added into fermentation broth together with vitamins, and the mixture is stirred uniformly to obtain the compound biological feed.
After the preparation of the compound biological feed is finished, the compound biological feed can be directly filled into a plastic barrel and stored in a sealing way, and can be packaged by a double-layer sealing plastic bag additionally arranged on the plastic barrel, wherein the storage time can reach 60 days, the compound biological feed does not generate acid, does not become alcohol or black. When the poultry is to be fed, the poultry can be directly taken out of the plastic bucket for feeding.
The application is further illustrated by the following examples. It is to be understood that these examples are illustrative of the present application and are not intended to limit the scope of the present application.
In the examples below, materials, reagents and apparatus used, unless otherwise specified, were commercially available.
Example 1
In this example, compound biological feeds having the following formulations were prepared, respectively, with the specific compositions shown in tables 1 and 2.
Table 1: component and dosage (g) of compound biological feed 1-6
Table 2: component and dosage (g) of compound biological feed 7-11
The preparation method of the composite biological feed comprises the following steps:
mixing bacillus subtilis, streptococcus faecalis, bifidobacterium, lactobacillus, saccharomycetes, xylo-oligosaccharide, calcium biphosphate and gallic acid in water according to a proportion, and fermenting at normal temperature for 10 hours to obtain a fermentation product;
pulverizing the nutritional composition (particle size is about 10 mm), adding into the fermented product together with vitamins, and stirring to obtain corresponding compound biological feed.
Example 2
2.1 the effect of the compound biological feed 1-11 prepared in the above example was verified, specifically including:
2.1.1 Bacillus subtilis count
After 1 day of storage at room temperature, the number of bacillus subtilis is detected according to the detection standard of bacillus subtilis in the GB/T26428-2010 feed microbial preparation. The test results are shown in Table 3.
Table 3: results of test on bacillus subtilis counts of feeds 3 and 7-11
| Test group | Bacterial count (CFU/g) |
| Feed 3 | 2×10 11 |
| Feed 7 | 4×10 10 |
| Feed 8 | 1×10 11 |
| Feed 9 | 3×10 10 |
| Feed 10 | 8×10 10 |
| Feed 11 | 2×10 10 |
According to the test results in Table 3, under the combined action of xylooligosaccharide, calcium dihydrogen phosphate and gallic acid, the proliferation and survival of bacillus subtilis are more facilitated. Compared with calcium dihydrogen phosphate, the proliferation effect of the xylo-oligosaccharide on bacillus subtilis is more obvious, and the presence of the gallic acid is more favorable for the xylo-oligosaccharide to exert the effect, and the gallic acid can promote the proliferation effect of the xylo-oligosaccharide on bacillus subtilis.
2.1.2 Effect on piglet growth Properties and diarrhea
110 piglets with good health condition and basically consistent weight (8.49+/-0.15 kg) are selected for the test. The piglets are randomly divided into 11 groups, are kept in the upper columns, are free to drink water, and have the same feeding amount and feeding time in daily feed (feed prepared at the same time), and are fed once in the morning and evening. After 21 days of continuous feeding, the piglets were checked regularly for diarrhea and tested for weight, the number of diarrhea in piglets was recorded and the average daily gain was calculated. The test results are shown in Table 4.
Table 4: piglet growth performance and diarrhea influence test result
| Test group | Average daily gain (kg/day) | Diarrhea number (head) of piglet |
| Feed 1 | 0.52 | 0 |
| Feed 2 | 0.53 | 0 |
| Feed 3 | 0.56 | 0 |
| Feed 4 | 0.52 | 0 |
| Feed 5 | 0.54 | 0 |
| Feed 6 | 0.50 | 0 |
| Feed 7 | 0.33 | 3 |
| Feed 8 | 0.40 | 1 |
| Feed 9 | 0.32 | 1 |
| Feed 10 | 0.37 | 1 |
| Feed 11 | 0.26 | 5 |
The test results in Table 3 and Table 4 are combined, and under the combined action of xylooligosaccharide, calcium dihydrogen phosphate and gallic acid, the feed 1-6 can obviously improve the activity and survival rate of probiotics, and has the effects of reducing the incidence of diarrhea of poultry and accelerating the growth and development of livestock.
When the feed 11 does not contain xylooligosaccharide, monocalcium phosphate and gallic acid, the activity and survival rate of probiotics are obviously reduced, so that the effects of improving the incidence of diarrhea of poultry and promoting the growth and development of the poultry are obviously reduced.
When the feed 7 only contains xylo-oligosaccharide, the xylo-oligosaccharide can only play a limited role in promoting the proliferation of probiotics such as bifidobacteria, bacillus subtilis and the like due to the lack of gallic acid, and meanwhile, the effect of promoting the growth and development of livestock and poultry and improving the intestinal health of the feed 7 is obviously reduced due to the lack of monocalcium phosphate.
Similarly, the feed 8 contains monocalcium phosphate and xylo-oligosaccharide, but the weight gain effect of the feed 8 can be obviously improved through the synergistic effect of the monocalcium phosphate and the xylo-oligosaccharide due to the lack of gallic acid. But the effect was reduced compared to feeds 1-6. It is also described herein that gallic acid can promote better action of xylo-oligosaccharides in the system of the present application.
According to the test results of the feed 9 and the feed 10, the activity and the survival rate of probiotics can be improved to a limited extent, and meanwhile, the effect of the gallic acid on the monocalcium phosphate is not obviously promoted; however, the effect of improving intestinal health of the feed 9 and the feed 10 is obvious due to the presence of gallic acid.
In conclusion, the method can improve the survival rate and the activity of the probiotics by screening the active general probiotics such as bacillus subtilis, streptococcus faecalis, bifidobacteria, lactobacillus and the like and adopting the xylooligosaccharide, the monocalcium phosphate and the gallic acid and through the combined action among the xylooligosaccharide, the monocalcium phosphate and the gallic acid, promote the digestion and absorption of the animal on the nutritional composition such as protein, cellulose and the like in the feed, reduce the diarrhea incidence of the poultry and improve the feed return.
The foregoing has described in detail the technical solutions provided by the embodiments of the present application, and specific examples have been applied to illustrate the principles and implementations of the embodiments of the present application, where the above description of the embodiments is only suitable for helping to understand the principles of the embodiments of the present application; meanwhile, as for those skilled in the art, according to the embodiments of the present application, there are variations in the specific embodiments and the application scope, and the present description should not be construed as limiting the present application.
Claims (10)
1. A compound biological feed, characterized in that it comprises, per 1000g of said compound biological feed:
0.1 to 2g of bacillus subtilis,
0.1 to 2g of streptococcus faecalis,
0.2 to 0.8g of bifidobacterium,
0.4 to 1.2g of lactic acid bacteria,
0.4 to 1.2g of saccharomycete,
0.1 to 0.6g of xylo-oligosaccharide,
10 to 20g of calcium dihydrogen phosphate,
gallic acid 0.4-1.2 g.
2. The compound biological feed of claim 1, comprising, per 1000g of the compound biological feed:
0.5 to 2g of bacillus subtilis,
0.2 to 1.6g of streptococcus faecalis,
0.3 to 0.8g of bifidobacterium,
0.4 to 0.8g of lactic acid bacteria,
0.4 to 0.8g of saccharomycete,
0.2 to 0.5g of xylo-oligosaccharide,
12-18 g of calcium dihydrogen phosphate,
gallic acid 0.5-1 g.
3. The compound biological feed of claim 1 or 2, further comprising, per 1000g of the compound biological feed:
400-500 g of water.
4. The compound biological feed of claim 3, further comprising a nutritional composition;
the nutritional composition is one or more selected from corn, soybean meal, wheat bran, peanut seedling powder and straw powder.
5. The compound biological feed of claim 4, further comprising vitamins;
the vitamins are one or more selected from vitamin B1, riboflavin, vitamin B6 hydrochloride, folic acid, vitamin K3, vitamin B12, vitamin A acetate, vitamin D3, vitamin E acetate and medium cellulose.
6. A method for increasing the viability of probiotics in a compound biological feed, the method comprising:
adding a combination of bacillus subtilis, streptococcus faecalis, bifidobacteria, lactobacillus, saccharomycetes, xylo-oligosaccharide and calcium dihydrogen phosphate into a compound biological feed;
wherein, every 1000g of the composite biological feed comprises the following components in percentage by weight:
0.1 to 2g of bacillus subtilis, 0.1 to 2g of streptococcus faecalis, 0.2 to 0.8g of bifidobacterium, 0.4 to 1.2g of lactobacillus, 0.4 to 1.2g of saccharomycete, 0.1 to 0.6g of xylo-oligosaccharide, 10 to 20g of calcium dihydrogen phosphate and 0.4 to 1.2g of gallic acid.
7. The method according to claim 6, wherein the contents of each component in each 1000g of the compound biological feed are:
0.5 to 2g of bacillus subtilis, 0.2 to 1.6g of streptococcus faecalis, 0.3 to 0.8g of bifidobacterium, 0.4 to 0.8g of lactobacillus, 0.4 to 0.8g of saccharomycete, 0.2 to 0.5g of xylo-oligosaccharide, 12 to 18g of calcium dihydrogen phosphate and 0.5 to 1g of gallic acid.
8. The method according to claim 6 or 7, characterized in that the method further comprises: adding water into the compound biological feed;
wherein, the water content in each 1000g of the composite biological feed is 400-500 g.
9. The method of claim 8, wherein the method further comprises: adding the nutritional composition to a compound biological feed;
the nutritional composition is one or more selected from corn, soybean meal, wheat bran, peanut seedling powder and straw powder.
10. The method of claim 8, wherein the method further comprises: adding vitamins into the compound biological feed;
the vitamins are one or more selected from vitamin B1, riboflavin, vitamin B6 hydrochloride, folic acid, vitamin K3, vitamin B12, vitamin A acetate, vitamin D3, vitamin E acetate and medium cellulose.
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| CN117461735A (en) * | 2023-11-02 | 2024-01-30 | 广东超聚和生物科技有限公司 | Feed composition with synergistic fermentation of bacterial enzymes and method for improving intestinal tracts of livestock and poultry by using feed composition |
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