Background technology
In the modern livestock and poultry cultivation process, in order to prevent and treat Animal diseases, often guarantee animal health with excessive antibiotic product, but excessive antibiotic use often causes the quality of animal meat product and product thereof to descend and the enhancing of antibiotic patience, and the transmission by food chain causes human health also to be subject to having a strong impact on of antibiotic problem.
Contain the several functions materials such as several amino acids, chlorogenic acid, vitamin, trace element in the folium cortex eucommiae, have spectrum antibiotic, improve animal body immunity and the speed of growth, improve the function of meat quality.
Contain polysaccharide, flavones, caffeinic derivative, essential oil, polyyne, alkylamine and alkaloid in the Echinacea, virus, bacterium and fungal infection disease that it is widely used in treating respiratory system suppress their breeding, stop its growth.Experimental results show that much Echinacea can the stimulating immune system cellular activity, it is by acting on macrophage and NK comes activating immune system.
Chinese mugwort powder (the cauline leaf drying and crushing by wormwood forms) nutritive value is very high, contains aromatic oil in the tarragon, can stimulate circulation, and strengthens metabolism, improves meat, and antibacterial action is arranged.
Purslane powder: mainly contain tea phenol, Polysaccharide from Portulaca oleracea, alkaloid and multivitamin etc., have desinsection detoxifcation, the loose blood of heat-clearing and bacteriostasis, can treat the infections of digestive canal such as enteritis diarrhea, the white scour of piglet.
Pine needle meal: contain several amino acids and vitamin, these nutritional labelings and bioactivator can promote growing of animal, improve output and the quality of livestock products.
Yeast selenium: keep the necessary trace element of body normal activities, in vivo with multiple biologically active form, participate in the adjusting of body different physiological roles, one of them important function is exactly to improve the immunity of body.Mechanisms of physiological role of selenium shows anti-oxidant, improves the aspects such as immunity and thyroid hormone conciliation mainly by the selenoprotein performance, is showed by the effect in their disease prevention and cure again.
Mushroom claims again mushroom, dried mushroom, is a kind of edible mushroom that human body is had stronger nutrition health-care functions.According to one's analysis, contain in the 100g fresh mushroom protein 18.4g, fatty 4.8g, carbohydrate 71g(wherein sugar account for 35.1g), and contain calcium 120mg, phosphorus 420mg, iron 25mg, vitamin B1 0.07mg, vitamin B2 1.13mg, and vitamin C, 30 plurality of enzymes and 16 seed amino acids, also contain in addition the compositions such as double stranded RNA, lentinan that rare ergot in the general vegetables stays alcohol (generating the precursor substance of vitamin D in the human body) and has immunologic function.
Contain in the mushroom leachate more can produce 5 of delicate flavour '-guanylic acid (5 '-GMP) and the mushroom smart (Lenthionine) that produces fragrance, several amino acids is produced the composition of distinguishing the flavor of with these and is mutually used, and has just produced the distinctive delicate flavour of mushroom and fragrance.Mushroom contains water-soluble delicate flavour material, can be used as food seasoning, so mushroom important edible and medical fungus and the flavouring that be people.Utilize the mushroom raw material, be equipped with suitable auxiliary material and still can process various delicious condiment, so both enriched the kind of food of fungi, improved again the economic benefit of mushroom.
Glossy ganoderma [Ganoderema lucidum(Leyss ex Fr.) karst], claim again polyporus lucidus, belong to Basidiomycotina, Aphyllophorales, Ganodermataceae, Ganoderma.Doctor's allusion quotation in successive dynasties playbacks glossy ganoderma in " medicine-feeding ", and its ranking is before ginseng.The record of Shennong's Herbal, Compendium of Material Medica, glossy ganoderma materials for improving vision, tonifying liver gas, calm the nerves, beneficial essence, the beneficial motive, beneficial temper, beneficial lung qi, kidney-nourishing gas, logical nine orifices, ear acute hearing, bright, the sharp joint of order, diuresis, skin maintenance, be first-class excellent tonic product.
GL-B can promote the synthetic of protein, nucleic acid, and to the renewal of serum, liver and bone marrow cell protein or nucleic acid, synthetic facilitation arranged, it is one of glossy ganoderma main active ingredient of strengthening the body resistance to consolidate the constitution.GL-B mainly is present in lucid ganoderma fungus fructification, sclerotium, mycelium or the zymotic fluid; Being present in ganoderic acid in the glossy ganoderma pain relieving, calmness, inhibition histamine releasing are arranged, detoxify, protect the liver, kill the function of tumour cell, is one of main active ingredient of glossy ganoderma.
Be derived from more natural natural materials and not only have good nutritive peculiarity, also have simultaneously antiviral, anti-inflammatory, anti-oxidant, regulate the effect such as body's immunity, have broad application prospects.
Summary of the invention
The technical problem that the present invention solves provides a kind of raising immunity of organisms composite Chinese herbal pig feed additive and preparation method.
Technical scheme of the present invention is as follows:
Improve immunity of organisms composite Chinese herbal pig feed additive, parts by weight consist of: Eucommia Leaf Powder 10-15 part; Echinacea powder 5-10 part; Wormwood powder 8-15 part; Purslane powder 10-20 part; Pine needle meal 8-15 part; Yeast selenium 3-6 part; Cellulase 5-8 part; Chinese herbal and crude drugs preparations 10-20 part; Bacillus subtilis (Bacillus subtilis) CGMCC6012 preparation 10-15 part; Herbal cuisine fungi 10-25 part.
Described herbal cuisine Fungal community composition is: mix making after the mushroom mushroom entity of the Ganoderma Lucidum fructification of 50-70% or mycelium and 30-50% is pulverized.
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
According to the preparation of following weight ratio, Radix Codonopsis 20-30%, fruit of Chinese wolfberry 10-20%, Radix Angelicae Sinensis 15-20%, Chinese cassia tree 15-25%, Radix Isatidis 5-10%, root of kudzu vine 10-20%, ginseng 10-15%; Be below 2 millimeters with being crushed to particle diameter after the above-mentioned mixing; Place container to add 2-4 times of water gaging mixed material, control temperature 50 C~70 ℃ keep 2~4h, and control temperature to 4 ℃~8 ℃ is added 1-2 times of weight ethanol of mixed material, keeps 2~3h, is warming up to 50 ℃~70 ℃ and keeps 4~6h, filters; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations.
Described bacillus subtilis is bacillus subtilis (Bacillus subtilis) CGMCC6012, and bacillus subtilis formulation is the freeze-dried vaccine powder, and number of viable is (0.1-5) * 10 in the bacterium powder
8Individual/g.
The invention provides a kind of production of fodder bacillus subtilis (Bacillus subtilis) bacterial classification, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 16th, 2012, the preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCC No6012.
Product preparation method of the present invention is as follows:
Bacillus subtilis formulation, Chinese herbal and crude drugs preparations, herbal cuisine fungi, Eucommia Leaf Powder, Echinacea powder, wormwood powder, purslane powder, pine needle meal, yeast selenium and the cellulase of freeze-drying preparation are mixed.
Technique scheme of the present invention has the following advantages compared to existing technology:
The present invention is composite by science, bacillus subtilis, glossy ganoderma, Chinese herbal and crude drugs preparations etc. have been realized organic assembling, particularly added traditional Chinese medicine ingredients, product of the present invention can effectively reduce the use amount of antibiotics in letting animals feed, improve the security of animal meat product, improve the food utilization efficiency of animal, strengthen the immunity of animal, improve raise benefit.
Bacillus subtilis has following function: 1. oxygen consumption produces acid, and the regulating intestinal canal microecological balance reaches the diseases such as prevention diarrhoea, diarrhea; 2. secrete multiple enzyme, improve the feed digestive utilization ratio; 3. generation various nutrients participates in body metabolism; 4. raising animal immunizing power.
The hydrolysis of described cellulase energy catalyse cellulose generates short fiber, cellobiose, glucose etc.As feed addictive, can improve feed digestibility, obviously improve gain in weight, milk production and the egg production of livestock and poultry, and improve disease-resistant and ability that conform.
Composite by the science of microorganism formulation bacillus subtilis, Ganoderma Lucidum, Chinese herbal and crude drugs preparations, enzyme preparation and natural material among the present invention, it is low effectively to solve the animal immunizing power that exists in the present feed cultivation, antibiotic dosage is too much, the problem that product quality descends.Use product of the present invention that animal immunizing power is improved a lot, antibiotic dosage reduces more than 60%, and animal product taste and meat have obvious lifting, and the organoleptic test evaluation shows that the animal product taste of use invention product and the evaluation of meat divide raising more than 65%.
The specific embodiment
Form and technique below by specific embodiment narration product of the present invention.Unless stated otherwise, used technological means is method known in those skilled in the art among the present invention.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes or change that separation and Extraction condition in these embodiments is carried out also belong to protection scope of the present invention.
Example 1: prepare 100 kilograms of products;
Improve immunity of organisms composite Chinese herbal pig feed additive, parts by weight consist of: 12 parts of Eucommia Leaf Powders, 8 parts in Echinacea powder, 10 parts in wormwood powder, 15 parts in purslane powder, 10 parts of pine needle meals, 5 parts of yeast seleniums, 7 parts of cellulases, 15 parts of Chinese herbal and crude drugs preparationses, 12 parts in bacillus subtilis (Bacillus subtilis) CGMCC6012 preparation, 20 parts of herbal cuisine fungies.
Described herbal cuisine Fungal community composition is: mix making after 60% Ganoderma Lucidum fructification and 40% mushroom mushroom entity are pulverized.
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
According to the preparation of following weight ratio, Radix Codonopsis 21%, the fruit of Chinese wolfberry 12%, Radix Angelicae Sinensis 17%, Chinese cassia tree 19%, Radix Isatidis 8%, the root of kudzu vine 12%, ginseng 11%; Be below 1 millimeter with being crushed to particle diameter after the above-mentioned mixing; Place container to add 3 times of water gagings mixed material, the control temperature 60 C keeps 3h, and control temperature to 6 ℃ is added 1.5 times of weight ethanol of mixed material again, keeps 2h, is warming up to 65 ℃ and keeps 5h, filters; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations.
Described bacillus subtilis is bacillus subtilis CGMCC6012, and number of viable is 2*10 in the freeze-dried vaccine powder
8Individual/g.
Product preparation method of the present invention is as follows:
Freeze-drying is prepared bacillus subtilis formulation, Chinese herbal and crude drugs preparations, herbal cuisine fungi, Eucommia Leaf Powder, Echinacea powder, wormwood powder, purslane powder, pine needle meal, yeast selenium and cellulase to be mixed and gets final product.
Bacillus subtilis formulation obtains:
Induction mutation of bacterium: take the ultraviolet mutagenesis method: take CICC10023 as starting strain, make bacteria suspension, coat on the culture medium plate, cultivate in 37 ℃ of incubators behind the ultraviolet irradiation 90s.
Bacterial screening: choose 30 single bacterium colonies of looking strong from the 90s plate, be inoculated on the inclined-plane, in 37 ℃ of incubators, cultivated 24 hours, 0--4 ℃ stored refrigerated; With original strain in contrast, adopt the Conventional herd breeding condition, sodium carboxymethylcellulose is as carbon source and proteolysis circle method screening cellulase-producing and the strong bacterial strain of protease, screening obtains cellulase-producing and strong bacterial strain bacillus subtilis (Bacillus subtilis) L1 of protease, this bacterial strain cellulase-producing can improve 25% by the force rate original strain, produces protease and can improve 52% by the force rate original strain.In the common micro-organisms center preservation of Chinese common micro-organisms culture presevation administration committee, deposit number is CGMCC No 6012 with this bacterial classification.
The preparation of bacillus subtilis microbial agent:
1. the acquisition of zymotic fluid: adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: bacillus subtilis CGMCC6012 slant strains is accessed in 500 ml shake flasks 100 milliliters of culture medium loading amounts, 180 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks 1000 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: with the first class seed pot of three grades of seeds take 10% inoculum concentration access total measurement (volume) as 150L, fermentation medium loading amount 100L, 28 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours;
(5) fermented and cultured: the first class seed pot bacterial classification is accessed total measurement (volume) as 1.5 tons of secondary seed tanks take 10% inoculum concentration, 1 ton of fermentation medium loading amount, 28 ℃ of condition of culture cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours is cultivated and is finished bacteria concentration at 11 * 108/ml.
(6) centrifugation: adopt centrifuge to separate and obtain thalline.
(7) vacuum freeze drying: adopt drying process with atomizing to process the acquisition dry bacteria after adding protective agent; Protective agent consists of defatted milk 10%, lactose 5%, glycerine 5%.Vacuum freeze-drying technique is write the culture presevation handbook referring to Institute of Microorganism, Academia Sinica, publishes in 1980. 610-653.
Culture medium forms: peptone 5 grams; Beef extract 3 grams; Sodium chloride 5 grams; Agar 20 grams; 1000 milliliters of distilled water; PH7.0.
Described cellulase is available from the grand mcroorganism Engineering Co., Ltd in Shandong.
Example 2: substantially with 100 kilograms of products of example 1 preparation
Improve immunity of organisms composite Chinese herbal pig feed additive, parts by weight consist of: 10 parts of Eucommia Leaf Powders; 10 parts in Echinacea powder; 8 parts in wormwood powder; 20 parts in purslane powder; 8 parts of pine needle meals; 3 parts of yeast seleniums; 5 parts of cellulases; 20 parts of Chinese herbal and crude drugs preparationses; 10 parts in bacillus subtilis (Bacillus subtilis) CGMCC6012 preparation; 25 parts of herbal cuisine fungies.
Described herbal cuisine Fungal community composition is: mix making after the mushroom mushroom entity of 50% Ganoderma Lucidum fructification or mycelium and 50% is pulverized.
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
According to the preparation of following weight ratio, Radix Codonopsis 30%, the fruit of Chinese wolfberry 10%, Radix Angelicae Sinensis 15%, Chinese cassia tree 15%, Radix Isatidis 10%, the root of kudzu vine 10%, ginseng 10%; Be below 2 millimeters with being crushed to particle diameter after the above-mentioned mixing; Place container to add 3 times of water gagings mixed material, the control temperature 60 C keeps 3h, and control temperature to 4 ℃ is added 2 times of weight ethanol of mixed material, keeps 2h, is warming up to 70 ℃ and keeps 6h, filters; Filtrate Vacuum Concentration postlyophilization obtains Chinese herbal and crude drugs preparations.
Described bacillus subtilis is bacillus subtilis CGMCC6012, and number of viable is 4*10 in the freeze-dried vaccine powder
8Individual/g.
Product preparation method of the present invention is as follows:
Freeze-drying is prepared bacillus subtilis formulation, Chinese herbal and crude drugs preparations, herbal cuisine fungi, Eucommia Leaf Powder, Echinacea powder, wormwood powder, purslane powder, pine needle meal, yeast selenium and cellulase to be mixed and gets final product.
Example 3: method is substantially with 100 kilograms of products of example 1 preparation
Improve immunity of organisms composite Chinese herbal pig feed additive, parts by weight consist of: 15 parts of Eucommia Leaf Powders; 5 parts in Echinacea powder; 15 parts in wormwood powder; 10 parts in purslane powder; 15 parts of pine needle meals; 6 parts of yeast seleniums; 8 parts of cellulases; 10 parts of Chinese herbal and crude drugs preparationses; 10 parts in bacillus subtilis (Bacillus subtilis) CGMCC6012 preparation; 25 parts of herbal cuisine fungies.
Described herbal cuisine Fungal community composition is: mix making after the mushroom mushroom entity of 70% Ganoderma Lucidum fructification or mycelium and 30% is pulverized.
The preparation method of described Chinese herbal and crude drugs preparations is as follows:
According to the preparation of following weight ratio, Radix Codonopsis 20%, the fruit of Chinese wolfberry 10%, Radix Angelicae Sinensis 20%, Chinese cassia tree 20%, Radix Isatidis 5%, the root of kudzu vine 15%, ginseng 10%;
Described bacillus subtilis is bacillus subtilis CGMCC6012, and number of viable is 1*10 in the freeze-dried vaccine powder
8Individual/g.
The result of use experiment
The result of use test of the inventive example 1 feed addictive in weanling pig
Test method:
It is 120 of the healthy weanling pigs of (7.16 ± 0.15) kg that experimental animal is selected Guangnan (Zhanjiang) subordinate plant of rich feed corporation,Ltd of family average weight, and statistical analysis body weight difference is not remarkable.Adopt the single-factor Randomized Designs, with 120 healthy weanling pigs, by male and female half and half, be divided into 2 groups (control group and test group), every group of 6 repetitions, each repeats 10 pigs.Test group is added the product of the present invention that embodiment 1 makes, and control group does not add product of the present invention.Fed continuously 30 days, and compared with control group, use the invention feed addictive can obtain following effect:
Project |
Control group |
Test group |
Deviation |
Starting weight (kg) |
7.09 |
7.23 |
-- |
End heavy (kg) |
18.34 |
19.95 |
-- |
Daily gain (g/d ﹒ head) |
375 |
424 |
13% |
Daily ingestion amount (g/d ﹒ head) |
607.5 |
627.5 |
3.3% |
Feedstuff-meat ratio |
1.62 |
1.48 |
-8.6% |
Diarrhea rate (%) |
14 |
2 |
-85.7% |
Result of the test shows, adds this product in the weanling pig daily ration, and the daily gain contrast group of piglet improves a lot; Feedstuff-meat ratio has obvious reduction; Diarrhea rate has reduced by 85.7%.
The result of use test of the inventive example 1 feed addictive in milking sow
Rich feed corporation,Ltd of (Zhanjiang) family has carried out by a definite date 21 days feeding experiment in the subordinate pig farm in the Guangnan.Single factor contrast design is adopted in test, chooses at random milking sow 30 health, that a farrowing number is close with the birth counterpoise, that parity is 2 or 3 tires, is divided at random 2 groups (being test group and control group), every group of 15 repetitions.Wherein: the test group daily ration adds this product that is prepared into by embodiment 2, and control group does not add product of the present invention.Record weaned piglet weight, diarrhea rate, the death rate.
Project |
Test group |
Control group |
Deviation |
Produce the young number (number) of living |
10.8 |
11.0 |
-- |
Birth counterpoise (kg) |
1.46 |
1.48 |
-- |
21 days small weaning pigs (head) |
10 |
9 |
11.1% |
21 days weight of weaning litters (kg) |
66.4 |
48.2 |
37.8% |
21 days wean counterpoises (kg) |
6.64 |
5.35 |
24.1% |
Net gain (kg) |
5.18 |
3.87 |
33.8% |
Daily gain (g) |
246 |
184 |
33.6% |
Grice diarrhoea rate (%) |
6.1 |
14.3 |
-57.3% |
The death rate (%) |
7.4 |
18.2 |
-59.3% |
Test shows: test group contrast group weight of weaning litter can improve 37.8%, and a piglet daily gain improves 33.6%, and diarrhea rate reduces 57.3%, and the death rate reduces by 59.3%.
The above results shows that product of the present invention can improve sow and piglet body immunity, increases the cultivation quality and benefits.