CN110024614A

CN110024614A - The method of container culture Antrodia camphorata fructification

Info

Publication number: CN110024614A
Application number: CN201810030446.7A
Authority: CN
Inventors: 邱德晃
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-01-12
Filing date: 2018-01-12
Publication date: 2019-07-19

Abstract

The invention discloses a kind of method of container culture Antrodia camphorata fructification, step includes: to prepare water, Cinnamomum kanahirai hay leaf, cinnamomum kanehirai sawdust, glucose, malt extract, peptone and milk powder, and the above material is mixed into the culture solution of liquid；Culture solution is taken out, and brown rice or rice germ is added and adds that cinnamomum kanehirai sawdust is cooked to form culture medium again, the culture medium is placed in container after culture medium is cooling；Antrodia camphorata mother bacterium is placed on the culture medium in the container under aseptic conditions, and is cultivated 45 days, Antrodia camphorata mother bacterium can grow up to antrodia mycelia；And temperature is then improved again, it cultivates 75 to 120 days, antrodia mycelia can be cultivated as Antrodia camphorata fructification；By the above method, it can reach paucibacillary cultivation environment and nutrient be easier to the practical effect being absorbed and utilized.

Description

The method of container culture Antrodia camphorata fructification

Technical field

The present invention relates to a kind of cultural methods of mushroom, especially with respect to a kind of side of container culture Antrodia camphorata fructification Method.

Background technique

The culture of known Antrodia camphorata fructification has TaiWan, China patent of invention, Application No. the 97114761st, invents Title: the artificial cultivation method of Antrodia camphorata mainly includes: providing appropriate culture medium, which is comprising one kind or one Kind or more comprising rice, millet, sorghum or wheat five cereals, 10% to 35% (V/V) coconut water, root crop, 10% to 30% (W/V) sugar；Wherein, the ratio of five cereals is about that 1 liter of culture medium about uses 0.5 to 1.5 kilogram of five cereals；Root The culture medium that the ratio of stem class crop is about 1 liter about uses 10 to 50 g of root crops；It is inoculated with Antrodia camphorata strain, and makes it It is grown under 23.0 ~ 27.0 DEG C of condition of culture；It is entirely after Antrodia camphorata strain covers after culture medium, adjustment cultivation temperature is 28.0 ~ 32.0 DEG C are continued culture to growing fructification；And collect Antrodia camphorata product.However, the artificial cultivation of above-mentioned Antrodia camphorata Method has missing below:

1. culture medium is comprising one or more kinds of five cereals comprising rice, millet, sorghum or wheat, those additions are It will be using the starch component of be rich in nutrition in the five cereals of rice, millet, sorghum or wheat, however, it is without maturation Starch will make Antrodia camphorata mother bacterium relatively be difficult to be absorbed and utilized in fact, that is, fail the five cereals of rice, millet, sorghum or wheat The nutrient of starch does best utilization.

2. those additions are the five cereals of rice utilize, millet, sorghum or wheat without sterilization processing, it is contained carefully Bacterium can grow up therewith in cultivating process and rob the nutrient of the culture medium in container, and make Antrodia camphorata mother bacterium can not be by cultivating Base obtains that institute is nutritious to grow up.

Summary of the invention

Present invention is primarily intended to be to solve the above problems, and provide a kind of side of container culture Antrodia camphorata fructification Method has more preferably practical effect, major technique, purpose are as follows: culture solution is by water, the Cinnamomum kanahirai hay leaf of crushing, Cinnamomum kanahirai hay trees Bits, glucose, malt extract (maltodextrin), peptone and milk powder mix, and brown rice or rice germ is added and adds again Add the cinnamomum kanehirai sawdust cooked culture medium for forming Antrodia camphorata of high temperature together, to which culture medium is placed in container after culture medium cooling In, by the culture medium containing Cinnamomum kanahirai hay leaf, cinnamomum kanehirai sawdust, cooked brown rice or rice germ, Antrodia camphorata mother bacterium is made to be easy to inhale Nutrition is received come the effect of growing into fructification.

Above-mentioned purpose according to the present invention provides a kind of method of container culture Antrodia camphorata fructification, and step includes: (a) Prepare the water of 1 liter of capacity, the Cinnamomum kanahirai hay leaf of 100 g of weight of crushing, 30 g of weight of cinnamomum kanehirai sawdust, 80 g of Portugals Grape sugar, 80 g of malt extracts (maltodextrin), 20 g of peptones and 20 g of milk powder, the above material is mixed into The culture solution of liquid；(b) 80 milliliters of culture solutions are taken out, and 100 g of brown rice or rice germ is added and adds 50 g of Cinnamomum kanahirai hays again Trees bits one, which arise to be cooked under 100 ~ 110 degree of high temperature Celsius, forms culture medium, is placed in the culture medium after culture medium is cooling In container；(c) Antrodia camphorata mother bacterium is placed on the culture medium in the container under aseptic conditions, and is trained at a temperature of 26 degree Celsius It supports 45 days, Antrodia camphorata mother bacterium can grow up to antrodia mycelia；And temperature (d) is then improved again to training at a temperature of 28 ~ 32 degree Celsius It supports 75 to 120 days, antrodia mycelia can be cultivated as Antrodia camphorata fructification.

In an embodiment of the present invention, wherein the Cinnamomum kanahirai hay leaf and cinnamomum kanehirai sawdust are ground into less than 0.2 square centimeter Clast.

By the above method, it can reach paucibacillary cultivation environment and growth nutrient be easier to the practical function being absorbed and utilized Effect.

Detailed description of the invention

Fig. 1 is the flow chart of the manufacturing method of the present invention.

Specific embodiment

To make to be further understood that the present invention, now lifts a preferred embodiment and cooperates schema, details are as follows:

Refering to Figure 1, its step includes: the cinnamomum kanehirai that (a) prepares the water of 1 liter of capacity, 100 g of weight of crushing Leaf, 30 g of cinnamomum kanehirai sawdust, 80 g of glucose, 80 g of malt extracts (maltodextrin), 20 g of peptones and The above material, is mixed into the culture solution of liquid by 20 g of milk powder；(b) 80 milliliters of culture solutions are taken out, and are added 100 g Brown rice or rice germ and add again 50 g of cinnamomum kanehirai sawdusts one arise under 100 ~ 110 degree of high temperature Celsius be cooked form culture medium, The culture medium is placed in container after culture medium is cooling；(c) Antrodia camphorata mother bacterium is placed in the container under aseptic conditions Culture medium on, and cultivated 45 days at a temperature of 26 degree Celsius, Antrodia camphorata mother bacterium can grow up to antrodia mycelia；And (d) then Temperature is improved again to cultivating 75 to 120 days at a temperature of 28 ~ 32 degree Celsius, and it is real as Antrodia camphorata can to cultivate antrodia mycelia Body；By the above method, reaches paucibacillary cultivation environment and growth nutrient is easier to the practical effect being absorbed and utilized.

Composition of the invention, specification specified are as follows: as shown in Figure 1, being the flow chart of the manufacturing method of the present invention, will first adopt The fresh Cinnamomum kanahirai hay leaf collected is crushed with 100 g of weight using pulverizer, it is preferable that the Cinnamomum kanahirai hay leaf and Cinnamomum kanahirai hay Trees are ground into the clast less than 0.2 square centimeter, make monoterpene contained by blade and Cinnamomum kanahirai hay trees script, sequiterpene, class Flavones, polysaccharide body, polyphenol, amino acid and a variety of vitamins and microelement, minerals are easy to release, then by the Cinnamomum kanahirai hay of crushing Leaf and 30 g of cinnamomum kanehirai sawdusts, 80 g of glucose, 80 g of malt extracts (maltodextrin), 20 g of peptones, 20 g of milk powder are mixed and added into the preparation for being mixed evenly in 1 liter of water and completing culture solution；Again by the culture solution Take out 80 milliliters, and 100 g of brown rice or rice germ is added and add 50 g of cinnamomum kanehirai sawdusts again into the culture solution and with 80 milliliter one arise under 100 ~ 110 degree of high temperature Celsius be cooked, formed can be used as culture Antrodia camphorata fructification culture medium, boiling In the process, it can kill except most bacterium, substantially reduce the culture medium containing bacterium number, and avoid other bacteriums and Antrodia camphorata female Bacterium mutually fights for the nutrient of the culture medium during cultivation, and meeting when the amylum body in cooked brown rice or rice germ sucks hot water It expands and ruptures, make Starch Hydrolysis and generate dextrin and the ingredient containing monosaccharide, and compare not cooked rice, cooked brown rice Or rice germ is easier to the effect of absorbing nutrient by Antrodia camphorata mother bacterium；Furthermore culture medium after cooling is placed in container bottle, and Ready Antrodia camphorata mother bacterium is placed on the culture medium in the container in desinfection chamber, and with 26 degree of growing environment cultures Celsius Grow up to antrodia mycelia after 45 days.Then, then change cultivation environment, make antrodia mycelia in 28 ~ 32 degree of growth rings Celsius It cultivates 75 to 120 days and Antrodia camphorata fructification can be grown up in border.

Embodiment described above is only to absolutely prove preferred embodiment that is of the invention and being lifted, not to limit to this The feature of invention, such as using the relevant technological means of the present invention, found creating again for principle, still fall within equivalent structure of the present invention Intention scope.

Claims

1. a kind of method of container culture Antrodia camphorata fructification, which is characterized in that step includes:

(a) water of 1 liter of capacity, the Cinnamomum kanahirai hay leaf of 100 g of weight of crushing, 30 g of cinnamomum kanehirai sawdusts, 80 g are prepared The above material, is mixed into the training of liquid by glucose, 80 g of malt extracts, 20 g of peptones and 20 g of milk powder Nutrient solution；

(b) 80 milliliters of culture solutions are taken out, and 100 g of brown rice or rice germ is added and adds 50 g of cinnamomum kanehirai sawdusts again together It is cooked under 100 ~ 110 degree of high temperature Celsius and forms culture medium, the culture medium is placed in container after culture medium is cooling；

(c) Antrodia camphorata mother bacterium is placed on the culture medium in the container under aseptic conditions, and is cultivated at a temperature of 26 degree Celsius 45 days, Antrodia camphorata mother bacterium grew up to antrodia mycelia；And

(d) temperature is then improved again to cultivating at a temperature of 28 ~ 32 degree Celsius 75 to 120 days, i.e. culture antrodia mycelia becomes Antrodia camphorata fructification.

2. the method for container culture Antrodia camphorata fructification as described in claim 1, which is characterized in that in step (a), the ox Camphortree leaf and cinnamomum kanehirai sawdust are ground into the clast less than 0.2 square centimeter.