CN109197382A - A kind of method that batch production prepares lentinus edodes strain stick - Google Patents
A kind of method that batch production prepares lentinus edodes strain stick Download PDFInfo
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- CN109197382A CN109197382A CN201811036805.6A CN201811036805A CN109197382A CN 109197382 A CN109197382 A CN 109197382A CN 201811036805 A CN201811036805 A CN 201811036805A CN 109197382 A CN109197382 A CN 109197382A
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- stick
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- lentinus edodes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Mycology (AREA)
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- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of methods that batch production prepares lentinus edodes strain stick, the present invention is by improving mushroom fungi, part sawdust is substituted using wheat bran, maize flour, wood resource has not only been saved, has saved production cost, and use the formula bacterium material mushroom culture, mycelial growth rate is fast, fruiting time shortens, and yield is high, and biological transformation ratio is high；The present invention also uses liquid spawn to carry out strain production, it is shorter with manufacturing cycle, the preparation cost of strain raw material is cheap, and inoculation efficiency is high, and bacterium germination is rapid after inoculation, the advantages that cover is fast, the strain production method time shortens 50 days or so compared with the inoculation of ordinary solid strain, improves operation speed, reduces workshop standby time, the batch production preparation for realizing each step, is worthy to be popularized.
Description
Technical field
The present invention relates to a kind of methods that breed of edible fungus technical field more particularly to batch production prepare lentinus edodes strain stick.
Background technique
Mushroom also known as fragrant bacterium, mushroom meat thickness is tender and crisp, delicious perfume (or spice), and edible medicinal value is higher, is known as " queen in mushroom ", it is deep by
People's likes, there is wide market development prospect, and it is the maximum edible mushroom of China's output that mushroom, which is known as the state mushroom of China,
Kind, counts according to edible fungi of china association, and China's mushroom total output in 2016 accounts for national edible mushroom total output up to 898.3 ten thousand t
24.97%.The artificial cultivation of mushroom originates from the dragon's fountain in Zhejiang Province of China, Qingyuan, Jingning county, is " chopping colored method ",
There is the history of nearly one thousand years.First chief Mr. Chen Meipeng of Edible Fungus Inst., Shanghai Academy of Agriculture in 1956 succeeds in developing
Mushroom sawdust pure culture, segment wood cultivated mushroom is successfully replaced followed by sawdust, has established scientific basic for modern cultivating champignon.
Nineteen eighty-two Fujian Province Gutian city peasant Peng Zhaowang has founded " cultivation of wood dust bacterium stick bag " technology, has been determined that China's lentinus edodes strain stick is planted substantially
The mode of training.
With the continuous development of China's economy labor cost rises year by year, edible mushroom industry enters transition and upgrade process.
Since environmental requirement is not achieved in small scall coal-fired boiler, the cultivation of mushroom is produced fast to factory culture from every family small workshop mode
Speed transformation.It invests mushroom plant and cultivates enterprise, the first problem faced is exactly the production of strain, directly buys the bacterium of strain plant
Kind quality is difficult to ensure that solid spawn expansion numerous period is long, influences enterprise's operation.Since mushroom strain culture uses sawdust as bacterium
Material, it is expensive, and the uneconomical environmental protection for, it is improved by the formula to mushroom fungi, by some agricultural productions
Product and by-product substitute pure sawdust, filter out excellent mushroom culture medium, not only economic and environment-friendly, but also to the high-quality height of mushroom
Production is of great significance, and can effectively push the development of mushroom industry.
Summary of the invention
The object of the invention is to remedy the disadvantages of known techniques, provides the side that a kind of batch production prepares lentinus edodes strain stick
Method.
The present invention is achieved by the following technical solutions:
A kind of method that batch production prepares lentinus edodes strain stick, comprising the following specific steps
(1) sawdust, wheat bran are prewetted in advance, then carries out maize flour, land plaster, potassium dihydrogen phosphate, white sugar in proportion
The preparation of Mixed Microbes material, adds water to water content 51-52% the 1st time, secondary to add water to water content 55% according to measurement result, adds water
It finishes, compost is passed to second level agitated kettle, is packed using Full-automatic sack filling machine, speed control is 500-600 packet/h, will be packed
Bacteria stick acanthopore afterwards pastes Breathable adhesive tape；
(2) it sterilizes at once after packing, after sterilizing, opens autoclave back door, residual steam is discharged into fore-cooling room,
Bacteria stick is directly pulled in cooling chamber to cool down, refrigeration machine is then reused and forces cooling, be cooled under certain temperature and be inoculated with, make
With full-automatic strain inoculation device, it is inoculated with speed 700-1000 stick/h, the bacteria stick after inoculation can paste transparent rubberized fabric；
(3) grid bedstead mode culture lentinus edodes strain stick is used, certain temperature and humidity are controlled, is carried out within 20-30 days after inoculation
1st acanthopore oxygenation, 40-45 days after inoculation, mycelia carries out the 2nd acanthopore oxygenation after covering with bacteria stick, controls bacterium after acanthopore oxygenation
Stick central temperature is no more than 28 DEG C, and light irradiation is given after the 2nd acanthopore oxygenation, promotes annesl；
(4) optimum growth temp for passing through the sunshade net of fruiting greenhouse, plastic film and cascade air-blower control mushroom, when bacteria stick turns
Color completely, high resilience when, bacteria stick reaches physiological maturity, can cut a bag fruiting at this time, 20-25 DEG C of bacteria stick is given after fruiting
Recuperation condition promotes mycelia to restore, and recuperation fills the water after 15-20 days, and stimulation bacteria stick is grown into next damp mushroom.
A kind of method that batch production prepares lentinus edodes strain stick, Mixed Microbes material described in step (1), specific preparation method is such as
Under: sawdust, wheat bran are carried out to prewet in advance stand-by, then maize flour, land plaster, potassium dihydrogen phosphate, white sugar are mixed, added
Enter in level-one agitated kettle, sawdust, wheat bran after prewetting uniformly is added in dry mixing afterwards, is uniformly mixed.
A kind of method that batch production prepares lentinus edodes strain stick, the proportion of Mixed Microbes material described in step (1) are as follows: sawdust 70%, bran
Skin 20%, maize flour 7%, land plaster 1%, potassium dihydrogen phosphate 1%, white sugar 1%.
A kind of method that batch production prepares lentinus edodes strain stick, sterilizing described in step (2) are specially steamed using double door high pressure
Vapour autoclave, 116-118 DEG C of high pressure sterilization.
A kind of method that batch production prepares lentinus edodes strain stick is cooled under certain temperature described in step (2) and is inoculated with, refers to
It is inoculated with to 18-20 DEG C, and is inoculated with using liquid spawn.
A kind of method that batch production prepares lentinus edodes strain stick controls wet 65-70% 22-23 DEG C of temperature control described in step (3).
A kind of method that batch production prepares lentinus edodes strain stick controls the optimum growth temp of mushroom described in step (4), refers to
10-25 DEG C of temperature control.
The invention has the advantages that the present invention is by improving mushroom fungi, it is wooden using wheat bran, maize flour substitution part
Bits, have not only saved wood resource, have saved production cost, and use the formula bacterium material mushroom culture, mycelial growth rate
Fastly, fruiting time shortens, and yield is high, and biological transformation ratio is high；The present invention also uses liquid spawn to carry out strain production, has preparation
The advantages that period is shorter, and the preparation cost of strain raw material is cheap, and inoculation efficiency is high, and bacterium germination is rapid after inoculation, and cover is fast, and it is general
Lead to solid spawn inoculation and shorten 50 days or so compared to the strain production method time, operation speed is improved, when reduction workshop is idle
Between, the batch production preparation of each step is realized, is worthy to be popularized.
Specific embodiment
A kind of method that batch production prepares lentinus edodes strain stick, comprising the following specific steps
(1) sawdust, wheat bran are prewetted in advance, then by maize flour, land plaster, potassium dihydrogen phosphate, white sugar by sawdust 70%,
Wheat bran 20%, maize flour 7%, land plaster 1%, potassium dihydrogen phosphate 1%, white sugar 1% ratio carry out Mixed Microbes material preparation, the 1st time plus
Water is secondary to add water to water content 55% according to measurement result to water content 51%, and water is added to finish, and compost is passed to second level stirring
Pot, is packed using Full-automatic sack filling machine, and speed control is 500 packets/h, by the bacteria stick acanthopore after pack, pastes Breathable adhesive tape；
(2) double door high-pressure steam sterilizing pan is used after packing at once, 116 DEG C of high pressure sterilizations, after sterilizing, opening is gone out
Bacterium pot back door, is discharged into fore-cooling room for residual steam, bacteria stick is directly pulled in cooling chamber and is cooled down, it is strong then to reuse refrigeration machine
Refrigeration but, is cooled to 18 DEG C, is inoculated with using full-automatic strain inoculation device, and using liquid spawn, and inoculation 700 stick of speed/
H, the bacteria stick after inoculation can paste transparent rubberized fabric；
(3) grid bedstead mode culture lentinus edodes strain stick is used, 22 DEG C of temperature control, controls wet 65%, the 1st acanthopore of progress in 20 days after inoculation
Oxygenation, 40 days after inoculation, mycelia carries out the 2nd acanthopore oxygenation after covering with bacteria stick, controls bacteria stick central temperature not after acanthopore oxygenation
More than 28 DEG C, light irradiation is given after the 2nd acanthopore oxygenation, promotes annesl；
(4) pass through 10 DEG C of optimum growth temp of the sunshade net of fruiting greenhouse, plastic film and cascade air-blower control mushroom, work as bacterium
Stick annesl completely, high resilience when, bacteria stick reaches physiological maturity, can cut a bag fruiting at this time, bacteria stick 20 is given after fruiting
DEG C recuperation condition, promote mycelia restore, recuperation 15 days after fill the water, stimulation bacteria stick enter next damp mushroom growth.
A kind of method that batch production prepares lentinus edodes strain stick, Mixed Microbes material described in step (1), specific preparation method is such as
Under: sawdust, wheat bran are carried out to prewet in advance stand-by, then maize flour, land plaster, potassium dihydrogen phosphate, white sugar are mixed, added
Enter in level-one agitated kettle, sawdust, wheat bran after prewetting uniformly is added in dry mixing afterwards, is uniformly mixed.
Claims (7)
1. a kind of method that batch production prepares lentinus edodes strain stick, which is characterized in that comprising the following specific steps
(1) sawdust, wheat bran are prewetted in advance, then carries out maize flour, land plaster, potassium dihydrogen phosphate, white sugar in proportion
The preparation of Mixed Microbes material, adds water to water content 51-52% the 1st time, secondary to add water to water content 55% according to measurement result, adds water
It finishes, compost is passed to second level agitated kettle, is packed using Full-automatic sack filling machine, speed control is 500-600 packet/h, will be packed
Bacteria stick acanthopore afterwards pastes Breathable adhesive tape；
(2) it sterilizes at once after packing, after sterilizing, opens autoclave back door, residual steam is discharged into fore-cooling room,
Bacteria stick is directly pulled in cooling chamber to cool down, refrigeration machine is then reused and forces cooling, be cooled under certain temperature and be inoculated with, make
With full-automatic strain inoculation device, it is inoculated with speed 700-1000 stick/h, the bacteria stick after inoculation can paste transparent rubberized fabric；
(3) grid bedstead mode culture lentinus edodes strain stick is used, certain temperature and humidity are controlled, is carried out within 20-30 days after inoculation
1st acanthopore oxygenation, 40-45 days after inoculation, mycelia carries out the 2nd acanthopore oxygenation after covering with bacteria stick, controls bacterium after acanthopore oxygenation
Stick central temperature is no more than 28 DEG C, and light irradiation is given after the 2nd acanthopore oxygenation, promotes annesl；
(4) optimum growth temp for passing through the sunshade net of fruiting greenhouse, plastic film and cascade air-blower control mushroom, when bacteria stick turns
Color completely, high resilience when, bacteria stick reaches physiological maturity, can cut a bag fruiting at this time, 20-25 DEG C of bacteria stick is given after fruiting
Recuperation condition promotes mycelia to restore, and recuperation fills the water after 15-20 days, and stimulation bacteria stick is grown into next damp mushroom.
2. the method that a kind of batch production according to claim 1 prepares lentinus edodes strain stick, which is characterized in that step (1) is described
Mixed Microbes material, it is specific the preparation method is as follows: sawdust, wheat bran are carried out in advance to prewet it is stand-by, then by maize flour, gypsum
Powder, potassium dihydrogen phosphate, white sugar are mixed, and are added in level-one agitated kettle, and sawdust, wheat bran after prewetting uniformly is added in dry mixing afterwards,
It is uniformly mixed.
3. the method that a kind of batch production according to claim 1 prepares lentinus edodes strain stick, which is characterized in that step (1) is described
Mixed Microbes material proportion are as follows: sawdust 70%, wheat bran 20%, maize flour 7%, land plaster 1%, potassium dihydrogen phosphate 1%, white sugar 1%.
4. the method that a kind of batch production according to claim 1 prepares lentinus edodes strain stick, which is characterized in that step (2) is described
Sterilizing, specially use double door high-pressure steam sterilizing pan, 116-118 DEG C of high pressure sterilization.
5. the method that a kind of batch production according to claim 1 prepares lentinus edodes strain stick, which is characterized in that step (2) is described
Be cooled under certain temperature and be inoculated with, refer to being inoculated at 18-20 DEG C, and be inoculated with using liquid spawn.
6. the method that a kind of batch production according to claim 1 prepares lentinus edodes strain stick, which is characterized in that step (3) is described
22-23 DEG C of temperature control, control wet 65-70%.
7. the method that a kind of batch production according to claim 1 prepares lentinus edodes strain stick, which is characterized in that step (4) is described
Control mushroom optimum growth temp, refer to 10-25 DEG C of temperature control.
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| CN201811036805.6A CN109197382A (en) | 2018-09-06 | 2018-09-06 | A kind of method that batch production prepares lentinus edodes strain stick |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111742783A (en) * | 2020-08-14 | 2020-10-09 | 唐湘梅 | Industrial production method of edible fungus sticks |
| CN112616560A (en) * | 2019-09-24 | 2021-04-09 | 吉林益隆长白山实业有限公司 | Mushroom liquid strain culture material and preparation method thereof |
| CN112806215A (en) * | 2021-03-23 | 2021-05-18 | 贵州大学 | Method for preparing mushroom liquid strain and method for producing mushroom stick |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593274A (en) * | 2017-10-31 | 2018-01-19 | 贵州省印江自治县梵净山生态菌业有限公司 | A kind of breeding method of mushroom |
-
2018
- 2018-09-06 CN CN201811036805.6A patent/CN109197382A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593274A (en) * | 2017-10-31 | 2018-01-19 | 贵州省印江自治县梵净山生态菌业有限公司 | A kind of breeding method of mushroom |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112616560A (en) * | 2019-09-24 | 2021-04-09 | 吉林益隆长白山实业有限公司 | Mushroom liquid strain culture material and preparation method thereof |
| CN111742783A (en) * | 2020-08-14 | 2020-10-09 | 唐湘梅 | Industrial production method of edible fungus sticks |
| CN112806215A (en) * | 2021-03-23 | 2021-05-18 | 贵州大学 | Method for preparing mushroom liquid strain and method for producing mushroom stick |
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