CN105175108A - Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus - Google Patents

Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus Download PDF

Info

Publication number
CN105175108A
CN105175108A CN201510512662.1A CN201510512662A CN105175108A CN 105175108 A CN105175108 A CN 105175108A CN 201510512662 A CN201510512662 A CN 201510512662A CN 105175108 A CN105175108 A CN 105175108A
Authority
CN
China
Prior art keywords
mushroom
pleurotus ostreatus
culture medium
cotton seed
sterilization
Prior art date
Application number
CN201510512662.1A
Other languages
Chinese (zh)
Inventor
陈翠翠
巩玉辉
宋芳芳
何洪胜
司昌雷
安志勇
隋海周
Original Assignee
青岛华盛绿能农业科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 青岛华盛绿能农业科技有限公司 filed Critical 青岛华盛绿能农业科技有限公司
Priority to CN201510512662.1A priority Critical patent/CN105175108A/en
Publication of CN105175108A publication Critical patent/CN105175108A/en

Links

Abstract

The invention belongs to the technical field of edible fungus culture, and specifically relates to a pleurotus ostreatus culture medium and a method using the same to culture pleurotus ostreatus. The provided pleurotus ostreatus culture medium is composed of the following raw materials in percentage by weight: 20 to 25% of cotton seed husk, 15 to 20% of barley husk, 40 to 48% of corn cob, 10 to 15% of wheat bran, 1 to 2% of gypsum, and 1 to 3% of lime. Wheat husk and corn cob are used to replace part of cotton seed husk as the main components of the pleurotus ostreatus culture medium; the circulation and reutilization of agricultural and sideline products are realized, the production cost of pleurotus ostreatus is greatly reduced, and moreover, the pleurotus ostreatus culture medium has more nutrients and higher medical value.

Description

A kind of oyster mushroom culture medium and the method with its cultivating white mushroom
Technical field
The invention belongs to fungus growing technique field.More specifically, the present invention relates to a kind of oyster mushroom culture medium and the method with its cultivating white mushroom.
Background technology
Flat mushroom have another name called pick up the ears, be Basidiomycota under the one of Agaricales Pleurotaceae, be kind of a quite common grey edible mushrooms.Flat mushroom is put down, and taste is sweet, has qi-restoratives, anticancer effect, can improve human metabolism, build up health, and regulating plant is neural.Flat mushroom contains polysaccharide body, has very strong restraining effect, and have immunological characteristic to tumour cell.The effect that flat mushroom also has wind-evil dispelling and cold-evil expelling, stimulates the circulation of the blood and cause the muscles and joints to relax, can control the diseases such as lumbago and skelalgia, numb in every limb, channels and collaterals are uncomfortable.In addition, flat mushroom is all effective in cure to hepatitis, chronic gastritis, stomach and duodenal ulcer, richets, hypertension etc., also has certain effect, can play opsonization to female dimacteric syndrome to reducing blood cholesterol and preventing and treating urethral calculus.Therefore, flat mushroom has good nutritive value and medical value, also has good economic worth simultaneously.
Along with the progress of science, the flat mushroom of the various different colours of modern artificial a large amount of cultivation is progressively become by wild domestication, in the 2000 multiple eating bacterium found both at home and abroad in the modern times, belong to growth rapidly, individual larger a kind of matter tender delicious, be worth very high large edible bacterium.
Substratum many employings cotton seed hulls of mushroom cultivation in prior art, in recent years due to the development of mushroom industry, the continuous increase of cotton seed hulls consumption, cause the price of cotton seed hulls also to rise steadily thereupon, price to cotton seed hulls in 2015 has risen to 2000 yuan/ton, the continuous increase of production cost, and economic benefit also reduces thereupon, therefore, the new problem that new substitutability raw material becomes cultivating white mushroom is found.
Summary of the invention
The present invention for a change prior art utilizes the high deficiency of cottonseed shell cultivation flat mushroom production cost, provides a kind ofly to utilize large wheat husk, the oyster mushroom culture medium of corn cob Substitute For Partial cotton seed hulls and the method with its cultivating white mushroom.
The present invention is achieved through the following technical solutions.
A kind of oyster mushroom culture medium, it is made up of the raw material of following mass percent: cotton seed hulls 20-25%, large wheat husk 15-20%, corn cob 40-48%, wheat bran 10-15%, gypsum 1-2%, lime 1-3%.
By a method for described substratum cultivating white mushroom, it comprises the steps:
(1) cotton seed hulls and the corn cob that take formula ratio are prewetted;
(2) add the large wheat husk of formula ratio, wheat bran, gypsum and lime to mix;
(3) add suitable quantity of water to control moisture at 60-65% in compound, stir laggard luggage bag, sealing;
(4) sterilising treatment is carried out;
(5), after end subject to sterilization, it is cooled;
(6) inoculate when temperature is down to room temperature;
(7) inoculate the complete culturing room that puts into cultivate.
In described step (1), in advance 24-48h carries out water pouring, turning prewets, and keeps prewetting moisture 65%.
In described step (3), edible fungus bag adopts polyethylene or polypropylene plastics pocket, when it is polyethylene plastic bag, in described step (4), sterilizing parameter is: atmospheric steam sterilizing, and sterilising temp is 100-105 DEG C, sterilization time is 10-12h, and the stewing time of putting is 6-8h; When it is polypropylene plastics pocket, in described step (4), sterilizing parameter is: high pressure steam sterilization, and sterilising temp is 121 DEG C-126 DEG C, and sterilization time is 2-4h, and the stewing time of putting is 6-8h.
Inoculation adopts one end to transplant the inoculation method of bar in described step (6), and carries out the inoculating process of front cover with flat mushroom solid spawn.
Culturing room's temperature controls at 20-26 DEG C in described step (7), and incubation time is 20-30 days, till mycelia sends out bacterium bag full.
The beneficial effect that the present invention compared with prior art has is:
(1) a kind of oyster mushroom culture medium provided by the invention, utilize large wheat husk, corn cob Substitute For Partial cotton seed hulls as main ingredient, achieve agricultural byproducts recycling capable of circulation, significantly reduce the production cost of flat mushroom, obtain huge economic benefit, ventilation property and the water resistance of substratum can be changed simultaneously;
(2) a kind of oyster mushroom culture medium provided by the invention, owing to containing part wheat bran in large wheat husk, vitamin B group enriches, and the large material rich cellulose of wheat husk own, easily absorbed by hypha of Pleurotus ostreatus, and good water retention can be played, thus indicate large wheat husk can cultivating white mushroom, this is not only the great achievement in research of an edible mushrooms industry, can also make tremendous contribution for significantly reducing flat mushroom production cost;
(3) a kind of oyster mushroom culture medium provided by the invention, the flat mushroom of cultivating has the advantages that mycelial growth is fast, growth cycle is short, output is high, and the flat mushroom leaf produced is large, meat is thick, and color glossiness is good;
(4) a kind of oyster mushroom culture medium provided by the invention, compared with the flat mushroom of cultivating using cotton seed hulls as main ingredient, there is more abundant amino acid, vitamin B group and anti-cancer polysaccharide, thus make the flat mushroom using this oyster mushroom culture medium to cultivate have better nutritive value and medical value.
Embodiment
Embodiment 1:
Control group and experimental group is divided to test in bacterium job contract factory of Qingdao Hua Shenglvneng agricultural science and technology company limited.
A kind of oyster mushroom culture medium, it comprises the component of following weight:
Control group: cotton seed hulls 88%, wheat bran 10%, gypsum 1%, lime 1%;
Experimental group: cotton seed hulls 25%, large wheat husk 15%, corn cob 48%, wheat bran 10%, gypsum 1%, lime 1%.
By a method for described substratum cultivating white mushroom, it comprises the following steps:
(1) cotton seed hulls, corn cob macrobead raw material in the flat mushroom base-material of formula ratio are shifted to an earlier date 24-48h and prewet, ensure that moisture is about 65%;
(2) take the cotton seed hulls of prewetting, the corn cob of formula ratio, then add large wheat husk, after wheat bran mixes, obtain compound;
(3) in the compound in step (2), add the water that the lime of formula ratio and gypsum, substratum siccative weigh 1.2 times, keep moisture at 60-65%, 40min is stirred at one-level stirrer, enter secondary blender again and carry out more than stirring 30min, then pack, adopt the polyethylene edible fungus bag of 22cm*40cm*5C, work loading height is 23cm, adopts three-piece (collar+lid+sponge) sealing;
(4) load sterilization basket, put on sterilizing car, car subject to sterilization is filled, and push Autoclave and carry out sterilizing, sterilizing parameter is: normal-pressure sterilization, and sterilising temp is 100 DEG C, and sterilization time is 12h, boils in a covered pot over a slow fire and puts time 6h;
(5), after sterilizing terminates, put into cooling room and cool;
(6) inoculate when bacterium bag is cooled to 25 DEG C, adopt the inoculation method of a bar of transplanting;
(7) inoculate the complete culturing room that puts into cultivate, culturing room's temperature 25 DEG C, cultivate about 20 days mycelia and cover with bacterium bag.
The oyster mushroom culture medium cost that the oyster mushroom culture medium that in the present embodiment prepared by experimental group is prepared than control group is low 0.58 yuan/bag, produce 10000 bags day, then can reduce costs 5800 yuan every day, the flat mushroom cultivated is compared with common flat mushroom, there is more abundant amino acid, vitamin B group and anti-cancer polysaccharide, therefore there is better nutritive value and medical value.Meanwhile, the flat mushroom of cultivating has that mycelial growth is fast, growth cycle is short, output is high, turns ultrafast feature, and the flat mushroom leaf produced is large, and glossiness is good.
Embodiment 2:
Control group and experimental group is divided to test in bacterium job contract factory of Qingdao Hua Shenglvneng agricultural science and technology company limited.
A kind of oyster mushroom culture medium, it comprises the component of following weight:
Control group: cotton seed hulls 88%, wheat bran 10%, gypsum 1%, lime 1%;
Experimental group: cotton seed hulls 20%, large wheat husk 20%, corn cob 48%, wheat bran 10%, gypsum 1%, lime 1%.
By a method for described substratum cultivating white mushroom, it comprises the following steps:
(1) cotton seed hulls, corn cob macrobead raw material in the flat mushroom base-material of formula ratio are shifted to an earlier date 24-48h and prewet, ensure that moisture is about 65%;
(2) take the cotton seed hulls of prewetting, the corn cob of formula ratio, then add large wheat husk, after wheat bran mixes, obtain compound;
(3) in the compound in step (2), add the water that the lime of formula ratio and gypsum, substratum siccative weigh 1.2 times, keep moisture at 60-65%, 40min is stirred at one-level stirrer, enter secondary blender again and carry out more than stirring 30min, then pack, adopt the polyethylene edible fungus bag of 22cm*40cm*5C, work loading height is 23cm, adopts three-piece (collar+lid+sponge) sealing;
(4) load sterilization basket, put on sterilizing car, car subject to sterilization is filled, and push Autoclave and carry out sterilizing, sterilizing parameter is: normal-pressure sterilization, and sterilising temp is 100 DEG C, and sterilization time is 12h, boils in a covered pot over a slow fire and puts time 6h;
(5), after sterilizing terminates, put into cooling room and cool;
(6) inoculate when bacterium bag is cooled to 25 DEG C, adopt the inoculation method of a bar of transplanting;
(7) inoculate the complete culturing room that puts into cultivate, culturing room's temperature 25 DEG C, cultivate about 20 days mycelia and cover with bacterium bag.
The oyster mushroom culture medium cost that the oyster mushroom culture medium that in the present embodiment prepared by experimental group is prepared than control group is low 0.64 yuan/bag, produce 10000 bags day, then can reduce costs 6400 yuan every day, the flat mushroom cultivated is compared with common flat mushroom, there is more abundant amino acid, vitamin B group and anti-cancer polysaccharide, therefore there is better nutritive value and medical value.Meanwhile, the flat mushroom of cultivating has that mycelial growth is fast, growth cycle is short, output is high, turns ultrafast feature, and the flat mushroom leaf produced is large, and glossiness is good.

Claims (6)

1. an oyster mushroom culture medium, is characterized in that, is made up of the raw material of following mass percent: cotton seed hulls 20-25%, large wheat husk 15-20%, corn cob 40-48%, wheat bran 10-15%, gypsum 1-2%, lime 1-3%.
2., by a method for substratum cultivating white mushroom described in claim 1, it is characterized in that, comprise the steps:
(1) cotton seed hulls and the corn cob that take formula ratio are prewetted;
(2) add the large wheat husk of formula ratio, wheat bran, gypsum and lime to mix;
(3) add suitable quantity of water to control moisture at 60-65% in compound, stir laggard luggage bag, sealing;
(4) sterilising treatment is carried out;
(5), after end subject to sterilization, it is cooled;
(6) inoculate when temperature is down to room temperature;
(7) inoculate the complete culturing room that puts into cultivate.
3. the method for cultivating white mushroom according to claim 2, is characterized in that, in described step (1), 24-48h carries out water pouring in advance, turning prewets, and keeps prewetting moisture 65%.
4. the method for cultivating white mushroom according to claim 2, it is characterized in that, in described step (3), edible fungus bag adopts polyethylene or polypropylene plastics pocket, when it is polyethylene plastic bag, in described step (4), sterilizing parameter is: atmospheric steam sterilizing, sterilising temp is 100-105 DEG C, and sterilization time is 10-12h, and the stewing time of putting is 6-8h; When it is polypropylene plastics pocket, in described step (4), sterilizing parameter is: high pressure steam sterilization, and sterilising temp is 121 DEG C-126 DEG C, and sterilization time is 2-4h, and the stewing time of putting is 6-8h.
5. the method for cultivating white mushroom according to claim 2, is characterized in that, in described step (6), inoculation adopts one end to transplant the inoculation method of bar, and carries out the inoculating process of front cover with flat mushroom solid spawn.
6. the method for cultivating white mushroom according to claim 2, is characterized in that, in described step (7), culturing room's temperature controls at 20-26 DEG C, and incubation time is 20-30 days, till mycelia covers with bacterium bag.
CN201510512662.1A 2015-08-20 2015-08-20 Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus CN105175108A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510512662.1A CN105175108A (en) 2015-08-20 2015-08-20 Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510512662.1A CN105175108A (en) 2015-08-20 2015-08-20 Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus

Publications (1)

Publication Number Publication Date
CN105175108A true CN105175108A (en) 2015-12-23

Family

ID=54897640

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510512662.1A CN105175108A (en) 2015-08-20 2015-08-20 Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus

Country Status (1)

Country Link
CN (1) CN105175108A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105732188A (en) * 2016-01-19 2016-07-06 严廷良 Culture medium for improving oyster mushroom nutritional components and reducing heavy metal and preparation method of culture medium
CN105766370A (en) * 2016-03-09 2016-07-20 沈成友 Method for utilizing blue-green algae for planting high-quality oyster mushrooms
CN106171525A (en) * 2016-07-28 2016-12-07 禄劝盛腾农业科技发展有限公司 The planting technique of edible fungi
CN106258490A (en) * 2016-08-17 2017-01-04 王瑜琳 A kind of cultural method of mushroom with abundant selenium
CN106748064A (en) * 2016-12-08 2017-05-31 河池市技术开发中心 It is a kind of to promote culture medium of elegant precious mushroom growth and preparation method thereof
CN108849238A (en) * 2018-06-29 2018-11-23 福建省永春县冠菌现代农业发展有限公司 Oyster mushroom inoculation method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101317532A (en) * 2007-06-06 2008-12-10 赵伟 Method for growing mushroom with corn core wheat hull briquetting
CN101870614A (en) * 2010-08-03 2010-10-27 李文胜 Edible mushroom culture medium
CN103598016A (en) * 2013-11-26 2014-02-26 黄秀英 Method for using raw materials to cultivate pleurotus eryngii
CN104829336A (en) * 2015-04-28 2015-08-12 吴中区胥口精益生物医药研究所 Mixed culture base material for pleurotus eryngii culture

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101317532A (en) * 2007-06-06 2008-12-10 赵伟 Method for growing mushroom with corn core wheat hull briquetting
CN101870614A (en) * 2010-08-03 2010-10-27 李文胜 Edible mushroom culture medium
CN103598016A (en) * 2013-11-26 2014-02-26 黄秀英 Method for using raw materials to cultivate pleurotus eryngii
CN104829336A (en) * 2015-04-28 2015-08-12 吴中区胥口精益生物医药研究所 Mixed culture base material for pleurotus eryngii culture

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105732188A (en) * 2016-01-19 2016-07-06 严廷良 Culture medium for improving oyster mushroom nutritional components and reducing heavy metal and preparation method of culture medium
CN105766370A (en) * 2016-03-09 2016-07-20 沈成友 Method for utilizing blue-green algae for planting high-quality oyster mushrooms
CN106171525A (en) * 2016-07-28 2016-12-07 禄劝盛腾农业科技发展有限公司 The planting technique of edible fungi
CN106258490A (en) * 2016-08-17 2017-01-04 王瑜琳 A kind of cultural method of mushroom with abundant selenium
CN106748064A (en) * 2016-12-08 2017-05-31 河池市技术开发中心 It is a kind of to promote culture medium of elegant precious mushroom growth and preparation method thereof
CN108849238A (en) * 2018-06-29 2018-11-23 福建省永春县冠菌现代农业发展有限公司 Oyster mushroom inoculation method

Similar Documents

Publication Publication Date Title
CN103355094B (en) A kind of method utilizing ramulus mori to cultivate white fungus
CN103387446B (en) A kind of planting almond abalone mushroom base and cultivating method thereof
CN103030468B (en) Oyster mushroom culture medium and oyster mushroom culture method using same
CN102187787B (en) Method for culturing rare edible fungi including tricholoma lobayense heim and clitocybe maxima by using vine shoot dust
CN101897273B (en) Coprinus comatus cultivating method and cultivating medium
CN101889522B (en) Method for planting pleurotus ostreatus by using shitake mushroom dregs
CN101627697B (en) Method for planting lentinus edodes by using mulberry branch wood chips as planting material
CN102577840B (en) Method for cultivating edible fungus with vinegar residue
CN103396205B (en) A kind of substratum of needle mushroom and cultivating method
CN102845219B (en) Cultivation technique of Pleurotus eryngii
CN101857489B (en) Zinc-enriched selenium-enriched lentinus edodes and cultivation method thereof
CN102557800B (en) Formula of special culture material capable of increasing content of each amino acid in grey mushroom fruiting body and culture method
CN102960184B (en) Preparation methodof edible fungus strain rich in organic selenium
CN103918475B (en) The elegant precious method of mushroom bonsai type cultivation and the medium for cultivating elegant precious mushroom
CN104938211A (en) Cultivation method for high yield and quality of mushroom
CN103518533B (en) Method for cultivating Zhijin bamboo fungus material zymophyte sticks
CN104303846B (en) Method with the big bag cultivating Pleurotus geesteranus of fermentation material
CN104478515B (en) Zymotic fluid, pleurotus eryngii culture medium and preparation method thereof for cultivating pleurotus eryngii matrix
CN102850116B (en) Needle mushroom cultivation medium and preparation method thereof
CN103250560A (en) Cultivating method of healthcare cassia seed contained coprinus comatus
CN104262024B (en) A kind of culture medium for golden mushroom and preparation method thereof
CN102318505B (en) Ganoderma lucidum potted landscape cultivation method
CN102786333B (en) Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same
CN106069185A (en) A kind of breeding method of Morchella esculenta (L.) Pers
CN103477869A (en) Method for utilizing mulberry branches to produce edible fungi

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20151223