CN107347446A - The cultural method of Cordceps militaris - Google Patents
The cultural method of Cordceps militaris Download PDFInfo
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- CN107347446A CN107347446A CN201710609745.1A CN201710609745A CN107347446A CN 107347446 A CN107347446 A CN 107347446A CN 201710609745 A CN201710609745 A CN 201710609745A CN 107347446 A CN107347446 A CN 107347446A
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- Prior art keywords
- cordceps militaris
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- culture medium
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- wheat
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention discloses a kind of cultural method of Cordceps militaris.This method comprises the following steps：(1) magnetized processing liquid culture medium；(2) Cordceps militaris strain is inoculated with aseptic condition；(3) the obtained strain of step (2) culture is sprayed in being cultivated on the culture medium containing only wheat；(4) illumination cultivation mycelia.The Cordceps militaris that the inventive method culture obtains, cordycepin content have great medical value and market value higher than the Cordceps militaris that existing cultural method is cultivated to obtain.
Description
Technical field
The invention belongs to food technology field, and in particular to a kind of cultural method of Cordceps militaris.
Background technology
Cordceps militaris, also known as northern Chinese caterpillar Fungus.The medicinal ingredient and content and medical value of Cordceps militaris can be equal to cordyceps sinensis
U.S., wherein cordycepin content are 3-5 times of cordyceps sinensis, and the content majority of other main components was higher than or equivalent to the worm summer in winter
Grass.
Cordycepin (cordycepin, i.e. 3'-Deoxyadenosine) is the characteristic chemical constituent of some Cordyceps sinensis fungus, and cordyceps sinensis
Belong to one of most important active component in fungi, it has the function such as immunological regulation, antitumor, antifatigue, regulation cardiopulmonary.Cordyceps sinensis
Element is as a kind of new broad-spectrum antibiotic, and with its distinctive antimicrobial antiviral activity, it can suppress the RNA synthesis of virus；It is right
Hay bacillus and mycobacterium tuberculosis avium have inhibitory action；Also there is lethal effect to HIV-I type viruses；Especially to a variety of solid malignants
Tumour has very strong inhibitory action.Cordycepin shows fabulous pharmaceutical applications prospect, and the research of cordycepin at present is now just turning into
An extremely active field in pharmaceutical chemistry.
Because Cordceps militaris has the medical value that can be compared favourably with cordyceps sinensis, and can be carried out by artificial cultivation a large amount of numerous
Grow, therefore, the artificial propagation amount of Cordceps militaris greatly increases, although cultivation improves entity yield currently on the market, but
It is to ignore the content for improving cordycepin in Cordceps militaris.
The content of the invention
For above-mentioned deficiency of the prior art, the present invention provides a kind of cultural method of Cordceps militaris, can effectively solve existing
Have in artificial cultivation, the problem of cordycepin content is not high.
A kind of cultural method of Cordceps militaris, comprises the following steps：
(1) add water stirring and dissolving potassium dihydrogen phosphate, in 100~120 DEG C, sterilize 0.5~1h, be subsequently cooled to 15~25
DEG C, then via 0.2~0.4T, 5~10h of magnetic treatment, obtain magnetized liquid culture medium；Wherein, the weight of potassium dihydrogen phosphate and water ratio
For 0.1:50~55；
(2) aseptically, Cordceps militaris strain is seeded in magnetized liquid culture medium, 100~180rpm cultures 3~
5 days；
(3) configuration comprises only the culture medium of wheat, adds water, and the 8~10h that sterilized in 100~120 DEG C, is cooled to 15~25
DEG C, then aseptically, the strain that step (2) culture obtains uniformly is sprayed on the culture medium for comprising only wheat, shape
It is incubated 4~5 days in 16~22 DEG C into the liquid spawn layer that thickness is 10mm, until mycelia is paved with wheat broth completely
Surface；Wherein, the weight of wheat and water ratio is 0.5:0.8~1；
(4) mycelia is irradiated 2~3 days with 500~600lx light intensity, daily 5~10h, treats that mycelia is all changed into by white
After crocus, irradiated with 500~600lx illumination whole day, continue 12~15 days, until stroma starts to grow, then with 500~
600lx monochromatic light exposures, daily 12~14h, after stroma grows 30~35 days, irradiated 20~25 days with 500~600lx light intensity
Afterwards, you can harvesting Cordceps militaris.
Further, the weight of potassium dihydrogen phosphate and water ratio is 0.1 in step (1):50.
Further, the magnetic field intensity of magnetic treatment is 0.4T in step (1).
Further, intensity of illumination is 500lx in step (4).
Further, monochromatic light is blue light, feux rouges, purple light, gold-tinted or green glow in step (4).
Further, monochromatic light is blue light in step (4).
Beneficial effects of the present invention are：
1st, potassium dihydrogen phosphate liquid culture medium is handled using 0.2~0.4T magnetic field intensity, forms magnetized liquid culture medium,
After magnetization, the hydrogen bond in fluid nutrient medium is destroyed, and larger water molecule group becomes less water molecule group, even
Single hydrone so that the interaction force between hydrone reduces, activity aggravation, and distance increases between molecule, and air contains
Oxygen amount increase so that fluid nutrient medium surface tension diminishes, osmotic pressure increases, solubility increases etc. so that contains in the aqueous solution
Ca2+、Mg2+、Fe2+、Zn2+、Cu2+、Ni+Deng being easier hydrated in metal ion liquid medium within, it is easy to it thin in strain
Transmission in the ion channel of intracellular, be advantageous to growing for strain, reach the average production rate of development of strain
4.21mm/d, its speed of growth improve 5.02% compared to existing training method.
2nd, the fluid nutrient medium after magnetizing, can effectively lift amylase, protease and the polyphenol during breeding strain
The activity of oxidizing ferment, decomposition absorbability of the enhancing strain to nutriment, to lift the yield of cordycepin in strain；Meanwhile
Magnetized fluid nutrient medium can be obviously improved the biological efficiency of strain, lift the accumulation of cordycepin and cordycepic acid in stroma
Content.
3rd, the fluid nutrient medium after magnetizing can also be obviously improved its absorptivity to illumination, especially to red, blue, purple light
Absorptivity greatly increases, and the enriching quantity of photon energy in culture medium is lifted, further to lift absorption of the strain to light quality.
4th, when strain generates stroma, using the blue light illumination culture of 500~600lx light intensity, it is remarkably improved strain
The accumulation of cordycepin in the yield and stroma of seat, then coordinate absorptivity increase of the magnetized liquid culture medium to light quality, enter one
Step strengthens absorption efficiency of the strain to blue light, the accumulation of cordycepin in the yield and stroma of stroma is reached maximum as far as possible
Value.
Embodiment
The embodiment of the present invention is described below, in order to which those skilled in the art understand this hair
It is bright, it should be apparent that the invention is not restricted to the scope of embodiment, for those skilled in the art,
As long as various change in the spirit and scope of the present invention that appended claim limits and determines, these changes are aobvious and easy
See, all are using the innovation and creation of present inventive concept in the row of protection.
Embodiment 1
A kind of cultural method of Cordceps militaris, comprises the following steps：
(1) add water stirring and dissolving potassium dihydrogen phosphate, in 100 DEG C, sterilize 1h, be subsequently cooled to 25 DEG C, then via 0.2T magnetic
5h is handled, obtains magnetized liquid culture medium；Wherein, the weight of potassium dihydrogen phosphate and water ratio is 0.1:55；
(2) aseptically, Cordceps militaris strain is seeded in magnetized liquid culture medium, 180rpm shaking table shaken cultivations
5 days；
(3) configuration comprises only the culture medium of 250g wheats, adds water 500g, and the 10h that sterilized in 100 DEG C, is cooled to 25 DEG C, so
Afterwards aseptically, the strain that step (2) culture obtains uniformly is sprayed on the culture medium for comprising only 250g wheats, formed
Thickness is 10mm liquid spawn layer, incubated 5 days in 22 DEG C, until mycelia is paved with wheat broth surface completely；
(4) wheat broth is irradiated 2 days with 500lx light intensity, daily 5h, treats that mycelia is all changed into crocus by white
Afterwards, irradiated with 600lx illumination whole day, continue 15 days, until stroma starts to grow, then with 600lx red light irradiation, daily 12
~14h, after stroma grows 30~35 days, after being irradiated 25 days with 580lx light intensity, you can harvesting Cordceps militaris.
Embodiment 2
A kind of cultural method of Cordceps militaris, comprises the following steps：
(1) add water stirring and dissolving potassium dihydrogen phosphate, in 100 DEG C, sterilize 1h, be subsequently cooled to 25 DEG C, then via 0.4T magnetic
10h is handled, obtains magnetized liquid culture medium；Wherein, the weight of potassium dihydrogen phosphate and water ratio is 0.1:50；
(2) aseptically, Cordceps militaris strain is seeded in magnetized liquid culture medium, 160rpm shaking table shaken cultivations
5 days；
(3) configuration comprises only the culture medium of 250g wheats, adds water 450g, and the 10h that sterilized in 100 DEG C, is cooled to 25 DEG C, will
The strain that step (2) culture obtains uniformly is sprayed on the culture medium for comprising only 250g wheats, forms the liquid that thickness is 10mm
Strain layer, it is incubated 5 days in 22 DEG C, until mycelia is paved with wheat broth surface completely；
(4) wheat broth is irradiated 3 days with 500lx light intensity, daily 8h, treats that mycelia is all changed into crocus by white
Afterwards, irradiated with 500lx illumination whole day, continue 15 days, until stroma starts to grow, then with 500lx blue light illumination, daily
14h, after stroma grows 35 days, after being irradiated 25 days with 500lx light intensity, you can harvesting Cordceps militaris.
Embodiment 3
A kind of cultural method of Cordceps militaris, comprises the following steps：
(1) add water stirring and dissolving potassium dihydrogen phosphate, in 100 DEG C, sterilize 1h, be subsequently cooled to 25 DEG C, then via 0.2T magnetic
8h is handled, obtains magnetized liquid culture medium；Wherein, the weight of potassium dihydrogen phosphate and water ratio is 0.1:52；
(2) aseptically, Cordceps militaris strain is seeded in magnetized liquid culture medium, 100rpm shaking table shaken cultivations
5 days；
(3) configuration comprises only the culture medium of 250g wheats, adds water 400g, and the 10h that sterilized in 100 DEG C, is cooled to 25 DEG C, will
The strain that step (2) culture obtains uniformly is sprayed on the culture medium for comprising only 250g wheats, forms the liquid that thickness is 10mm
Strain layer, it is incubated 4 days in 16 DEG C, until mycelia is paved with wheat broth surface completely；
(4) wheat broth is irradiated 2 days with 600lx light intensity, daily 5h, treats that mycelia is all changed into crocus by white
Afterwards, irradiated with 500lx illumination whole day, continue 12 days, until stroma starts to grow, then with 600lx blue light illuminations, daily 14h,
After stroma grows 30 days, after being irradiated 20 days with 500lx light intensity, you can harvesting Cordceps militaris.
Comparative example 1
Compared with Example 2, magnetization treatment is lacked in step (1), remaining operating process is same as Example 2.
Comparative example 2
Compared with Example 2, monochromatic light exposure is lacked in step (4), remaining operating process is same as Example 2.
Comparative example 3
Compared with Example 2, magnetization treatment is lacked in step (1), step lacks monochromatic light exposure in (4), remaining operation
Process is same as Example 2.
Detection
The embodiment 1~3 and comparative example 1~3 for weighing equivalent respectively cultivate obtained Cordceps militaris, detect under the same conditions
The wherein content of cordycepin, it the results are shown in Table 1.
The content of cordycepin in the Cordceps militaris that 1 different training methods of table are obtained
From the data in table 1, it can be seen that embodiment 1~3, which cultivates cordycepin content in obtained Cordceps militaris, is above comparative example 1~3,
Wherein especially with the cordycepin content highest in embodiment 2, and the cordycepin content in comparative example 1 and comparative example 2 is also above contrast
Example 3, therefore, by each step in inventive method and the cooperation of relevant parameter, it could cultivate and obtain with high content cordyceps sinensis
The Cordceps militaris of element, and the inventive method process is simple, the cultivation cost of Cordceps militaris is low, greatly improves the medical value of Cordceps militaris
And market value.
Claims (6)
1. a kind of culture process of Cordceps militaris, it is characterised in that comprise the following steps：
(1) plus water stirring and dissolving potassium dihydrogen phosphate, in 100~120 DEG C, 0.5~1h is sterilized, is subsequently cooled to 15~25 DEG C, then
Via 0.2~0.4T, 5~10h of magnetic treatment, magnetized liquid culture medium is obtained；Wherein, the weight of potassium dihydrogen phosphate and water ratio is
0.1:50~55；
(2) aseptically, Cordceps militaris strain is seeded in magnetized liquid culture medium, 3~5 is cultivated in 100~180rpm
My god；
(3) configuration comprises only the culture medium of wheat, adds water, and the 8~10h that sterilized in 100~120 DEG C, is cooled to 15~25 DEG C, so
Afterwards aseptically, the strain that step (2) culture obtains uniformly is sprayed on the culture medium for comprising only wheat, forms thickness
It is incubated 4~5 days in 16~22 DEG C for 10mm liquid spawn layer, until mycelia is paved with wheat broth surface completely；Its
In, the weight ratio of wheat and water is 0.5:0.8~1；
(4) mycelia 2~3 days, daily 5~10h is irradiated with 500~600lx light intensity, it is orange treats that mycelia is all changed into by white
After color, irradiated with 500~600lx illumination whole day, continue 12~15 days, until stroma starts to grow, then with 500~600lx
Monochromatic light exposure, daily 12~14h, after stroma grows 30~35 days, after being irradiated 20~25 days with 500~600lx light intensity, i.e.,
Cordceps militaris can be harvested.
2. the culture process of Cordceps militaris according to claim 1, it is characterised in that potassium dihydrogen phosphate described in step (1)
Weight ratio with water is 0.1:50.
3. the culture process of Cordceps militaris according to claim 1, it is characterised in that the magnetic of magnetic treatment described in step (1)
Field intensity is 0.4T.
4. the culture process of Cordceps militaris according to claim 1, it is characterised in that intensity of illumination is equal described in step (4)
For 500lx.
5. the culture process of Cordceps militaris according to claim 1, it is characterised in that monochromatic light described in step (4) is indigo plant
Light, feux rouges, purple light, gold-tinted or green glow.
6. the culture process of Cordceps militaris according to claim 5, it is characterised in that monochromatic light described in step (4) is indigo plant
Light.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109168992A (en) * | 2018-10-27 | 2019-01-11 | 太湖县金江源农业发展有限公司 | 238 mycelia of mushroom is improved in the method for cultivating seeds of bacteria phase heat resisting temperature |
| CN109964727A (en) * | 2019-04-22 | 2019-07-05 | 淮阴师范学院 | A method of Selenium-riched Cordyceps Militaris rate is improved using illumination |
| CN112568056A (en) * | 2020-12-08 | 2021-03-30 | 锦州聚红农业科技有限公司 | High-adenosine cordyceps militaris cultivation method |
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|---|---|---|---|---|
| CN109168992A (en) * | 2018-10-27 | 2019-01-11 | 太湖县金江源农业发展有限公司 | 238 mycelia of mushroom is improved in the method for cultivating seeds of bacteria phase heat resisting temperature |
| CN109964727A (en) * | 2019-04-22 | 2019-07-05 | 淮阴师范学院 | A method of Selenium-riched Cordyceps Militaris rate is improved using illumination |
| CN112568056A (en) * | 2020-12-08 | 2021-03-30 | 锦州聚红农业科技有限公司 | High-adenosine cordyceps militaris cultivation method |
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