CN103305448A - Mixed culture bacterial solution for quickly aging new pit of strong aromatic Chinese spirits and maintenance method - Google Patents
Mixed culture bacterial solution for quickly aging new pit of strong aromatic Chinese spirits and maintenance method Download PDFInfo
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Abstract
The invention discloses a mixed culture bacterial solution for quickly aging a new pit of strong aromatic Chinese spirits and a maintenance method, and belongs to the field of microorganisms. The mixed culture bacterial solution mainly contains 5-20 parts by weight of methanobacterium culture solution, 1-7 parts by weight of caproic acid bacterium culture solution and 15-30 parts by weight of liquid old pit mud; and the mixed culture bacterial solution is prepared through the following steps of: putting maternal draff, yellow water, medium-high temperature yeast for making hard liquor, yeast extract, sodium acetate and dipotassium phosphate into a fermentation container, performing heat treatment by using boiling water and closing the fermentation container; when the temperature in the fermentation container drops to 40 DEG C, adding the methanobacterium culture solution, the caproic acid bacterium culture solution and the liquid old pit mud as well as an alcohol to the container, full filling the fermentation container with cool boiled water and regulating the pH value to 6.5 to 7.0, and then cultivating at 33 to 37 DEG C. The mixed culture bacterial solution provided by the invention is capable of aging the pit mud more quickly and more efficiently; and therefore, a new method for improving the quality of Chinese spirits is provided.
Description
Technical field
The invention belongs to microorganism field, be specifically related to a kind of aromatic Chinese spirit fast aging mixed culture bacterium liquid and maintenance process of new cellar for storing things that make.
Background technology
Aromatic Chinese spirit is one of three large typical aromatic white spirits, and its unique mud cellar for storing things zymotechnique has been brought up distinctive local flavor.One of its flavor characteristic of giving off a strong fragrance liquor is that the cellar for storing things is aromatic strongly fragrant, and cellar for storing things mud microorganism is the main source of cellar for storing things Studies of The Aromatic Substances in the brewing process just.Constantly carrying out along with fermenting process in the production of giving off a strong fragrance liquor, cellar for storing things mud microorganism species kind and quantity constantly tend to balance with stable, finally reach its specific microbial bacteria group structure, and cellar for storing things mud reaches aging, tend towards stability thereby use the wine quality, make the more rich aromatic strongly fragrant sense in cellar for storing things of wine.
The Luzhou-flavor liquor pit mud quality good or not is determining product wine quality, therefore the aging particularly important of wine quality acceleration cellar for storing things mud for improving.Because traditional giving off a strong fragrance cellar for storing things mud natural aging is the time that needs many decades, yet cellar for storing things mud microbe species is various and metabolism is particularly complicated, therefore aging mechanism still can't be illustrated at present fully.
Giving off a strong fragrance White wine pit mud aging, basic reason be functional microorganism constantly instruction, flora structure mutually coordinate, finally set up the process of flora advantage.Cellar for storing things mud in long-term domestication process, gradually enrichment the relevant various functional microorganisms of a large amount of flavourings, microorganisms such as caproic acid bacteria, methagen, butyric bacteria, actinomycetes.
The present invention is by Manual propagation cellar for storing things mud functional microorganism, improves new cellar for storing things mud microbial bacteria group structure, achieves aging fast.For mixed culture bacterium liquid of the present invention and foster cellar for storing things method, have not yet to see relevant therewith research report.
Summary of the invention
Technical problem to be solved by this invention provides a kind of aromatic Chinese spirit fast aging mixed culture bacterium liquid and maintenance process of new cellar for storing things that make.
The technical solution adopted for the present invention to solve the technical problems is: make fast aging mixed culture bacterium liquid of the new cellar for storing things of aromatic Chinese spirit, comprising: the liquid old cellar for storing things mud of the methagen nutrient solution of 5～20 weight parts, the caproic acid bacteria nutrient solution of 1～7 weight part and 15～30 weight parts.
Further, above-mentioned mixed culture bacterium liquid comprises: the liquid old cellar for storing things mud of the methagen nutrient solution of 10～14 weight parts, the caproic acid bacteria nutrient solution of 3～5 weight parts and 18～25 weight parts.
Wherein, above-mentioned mixed culture bacterium liquid is to be made by following methods: female grain of 3～5 weight parts, the yellow water of 3～5 weight parts, the middle high-temperature daqu of 2～4 weight parts, the yeast extract paste of 0.05～0.2 weight part, the sodium-acetate of 0.1～0.3 weight part and the dipotassium hydrogen phosphate of 0.02～0.03 weight part are put into fermenting container, and the boiling water that adds again 30～50 weight parts carries out sterilising treatment and seals fermenting container; When the temperature in the fermenting container is down to 40 ℃, in container, add methagen nutrient solution, caproic acid bacteria nutrient solution, liquid old alcohol of storing mud and 1～3 weight part, fill up fermenting container with cold boiling water again and regulate pH value to 6.5～7.0, under 33～37 ℃ temperature, cultivated 20～30 days.
Wherein, in the above-mentioned mixed culture bacterium liquid, described methagen nutrient solution is to add yeast extract paste in the methagen liquid filter out cultivate bacterium liquid more than 30 days under 33～37 ℃ of temperature by cultivating to artificial eye-liquid.
Further, the weight of the yeast extract paste of interpolation is 0.5～1.5 ‰ of methagen liquid weight.
Wherein, in the above-mentioned mixed culture bacterium liquid, described caproic acid bacteria nutrient solution is to add yeast extract paste, sodium-acetate, alcohol and dipotassium hydrogen phosphate by cultivating in the caproic acid bacterial classification that filters out to artificial eye-liquid, re-adjustment pH value to 6.5～7.0, and liquid anaerobism is cultivated 7～10 days bacterium liquid under 35 ℃ of constant temperatures.
Further, take the weight of caproic acid bacterial classification as benchmark, the weight of adding yeast extract paste is 0.5～1.5 ‰, the weight of sodium-acetate is 4～6 ‰, the weight of alcohol is 1～3%, the weight of dipotassium hydrogen phosphate is 0.2～0.3 ‰.
Wherein, in the above-mentioned mixed culture bacterium liquid, it is with after 38～42 ℃ cold boiling water mixes by the weight ratio of 1 ︰ 0.8～1 with the old cellar for storing things mud of maturation that described liquid state is stored mud always, add again yeast extract paste, sodium-acetate and dipotassium hydrogen phosphate stirring and evenly mixing, pack into fermenting container and with alcohol to the sterilization of vessel port and liquid level, under 33～37 ℃ temperature, cultivate 3～5 days bacterium liquid.
Further, take the gross weight of old cellar for storing things mud and cold boiling water as benchmark, the weight of adding yeast extract paste is 0.5～1.5 ‰, the weight of sodium-acetate is 4～6 ‰, the weight of dipotassium hydrogen phosphate is 0.2～0.3 ‰.
The present invention also provides and makes aromatic Chinese spirit fast aging maintenance process of new cellar for storing things, dig cellar for storing things mud at the bottom of the cellar for storing things of cellar for storing things mud not aging in the pond, cellar for storing things or appearance degeneration loose, to dig loose cellar for storing things mud weight as benchmark, add 5～15% above-mentioned mixed culture bacterium liquid and dig loose cellar for storing things mud mixing, Jiao Chi is applied flat with the cellar for storing things mud behind the mixing again.
Wherein, every a bite Jiao Chi processes 2～3 times as stated above.
The invention has the beneficial effects as follows: the inventive method is the beneficial microorganism that adopts Manual propagation methagen, caproic acid bacteria, actinomycetes etc. relevant with producing wine, adopt the maintenance of polynary bacterium liquid, make the flora structure reach balance and optimization, thereby improve cellar for storing things mud microbial bacteria group structure, realize fast aging a kind of technology of cellar for storing things mud.The present invention makes aging more quick, the high-level efficiency of cellar for storing things mud, for the raising of liquor quality provides a kind of new method.
Embodiment
Below by embodiment the present invention is further detailed.
The present invention makes fast aging mixed culture bacterium liquid of the new cellar for storing things of aromatic Chinese spirit, comprising: the liquid old cellar for storing things mud of the methagen nutrient solution of 5～20 weight parts, the caproic acid bacteria nutrient solution of 1～7 weight part and 15～30 weight parts.
Preferably, above-mentioned mixed culture bacterium liquid comprises: the liquid old cellar for storing things mud of the methagen nutrient solution of 10～14 weight parts, the caproic acid bacteria nutrient solution of 3～5 weight parts and 18～25 weight parts.
Preferably, in order to make maintenance effect better, above-mentioned mixed culture bacterium liquid is to be made by following methods: female grain of 3～5 weight parts, the yellow water of 3～5 weight parts, the middle high-temperature daqu of 2～4 weight parts, the yeast extract paste of 0.05～0.2 weight part, the sodium-acetate of 0.1～0.3 weight part and the dipotassium hydrogen phosphate of 0.02～0.03 weight part are put into fermenting container, and the boiling water that adds again 30～50 weight parts carries out sterilising treatment and seals fermenting container; When the temperature in the fermenting container is down to 40 ℃, in container, add methagen nutrient solution, caproic acid bacteria nutrient solution, liquid old alcohol of storing mud and 1～3 weight part, fill up fermenting container with cold boiling water again and regulate pH value to 6.5～7.0, under 33～37 ℃ temperature, cultivated 20～30 days.
In the culturing process of above-mentioned mixed bacteria liquid, the effect of each main substance: 1) effect of female grain is for microorganism provides solid-liquid interface, is conducive to the wherein apposition growth of microorganism, provides certain nutritive substance for cellar for storing things mud microorganism simultaneously; 2) Main Function of yellow water is for the high organic acid of simulation cellar for storing things mud microorganism growth and the environment of alcoholic strength, turns out the beneficial microorganism that is suitable for storing the mud acid environment in culturing process, and certain nutritive substance also is provided simultaneously; 3) high-temperature daqu and yeast extract paste in, its Main Function are exactly that cultivation for mixed bacteria liquid provides the sufficient nutrient material, i.e. carbon source, nitrogenous source, inorganic salt etc.; 4) Main Function of sodium-acetate is, suppresses the breeding of the miscellaneous bacterias such as acetic bacteria by free acetate ion; 5) dipotassium hydrogen phosphate main shock absorption that plays potential of hydrogen in culturing process guarantees that namely mixed bacteria liquid acidity in culturing process keeps relative stability.Most importantly the mixing of above-mentioned these materials, the nutritive substances such as sufficient carbon source, nitrogenous source, inorganic salt not only are provided for cellar for storing things mud microbial growth, make the nutritive substance balance, and under the above-mentioned substance comprehensive action, make the mixing microorganisms of turning out well to be fit to the high organic acid environment of cellar for storing things mud.
The cultivation acidity environment that act as the balance mixing microorganisms of pH value to 6.5～7.0, because the optimal pH value of caproic acid bacteria is that about 6.0 (its pathways metabolism changed when pH was too high, pH crosses when low that its growth and breeding is suppressed even is dead), the optimal pH of methagen then is neutral meta-alkalescence, therefore the common cultivation of comprehensive above-mentioned two kinds of major microorganisms is adjusted to 6.5～7.0 with the pH value.Cultivated under 33～37 ℃ temperature 20～30 days, its effect is that microbe population is fully bred, and the microbial bacteria group structure is continued to optimize, and reaches or approach the flora structure of ripe old cellar for storing things mud microorganism; Fully ferment through the long period simultaneously and remove different assorted smell in the nutrient solution, guarantee to produce the pure of wine aroma when making it be used for supporting the cellar for storing things operation.
Preferably, better in order to make maintenance effect, in the above-mentioned mixed culture bacterium liquid, described methagen nutrient solution is to add yeast extract paste in the methagen liquid filter out cultivate bacterium liquid more than 30 days under 33～37 ℃ of temperature by cultivating to artificial eye-liquid.
Preferably, the weight of the yeast extract paste of interpolation is 0.5～1.5 ‰ of methagen liquid weight.
Preferably, in order to make maintenance effect better, in the above-mentioned mixed culture bacterium liquid, described caproic acid bacteria nutrient solution is to add yeast extract paste, sodium-acetate, alcohol and dipotassium hydrogen phosphate by cultivating in the caproic acid bacterial classification that filters out to artificial eye-liquid, re-adjustment pH value to 6.5～7.0, liquid anaerobism is cultivated 7～10 days bacterium liquid under 35 ℃ of constant temperatures.
Preferably, take the weight of caproic acid bacterial classification as benchmark, the weight of adding yeast extract paste is 0.5～1.5 ‰, the weight of sodium-acetate is 4～6 ‰, the weight of alcohol is 1～3%, the weight of dipotassium hydrogen phosphate is 0.2～0.3 ‰.
Preferably, in order to make maintenance effect better, in the above-mentioned mixed culture bacterium liquid, it is with after 38～42 ℃ cold boiling water mixes by the weight ratio of 1 ︰ 0.8～1 with the old cellar for storing things mud of maturation that described liquid state is stored mud always, add again yeast extract paste, sodium-acetate and dipotassium hydrogen phosphate stirring and evenly mixing, pack into fermenting container and with alcohol to the sterilization of vessel port and liquid level, under 33～37 ℃ temperature, cultivate 3～5 days bacterium liquid.
Preferably, take the gross weight of old cellar for storing things mud and cold boiling water as benchmark, the weight of adding yeast extract paste is 0.5～1.5 ‰, the weight of sodium-acetate is 4～6 ‰, the weight of dipotassium hydrogen phosphate is 0.2～0.3 ‰.
The present invention also provides and makes aromatic Chinese spirit fast aging maintenance process of new cellar for storing things, dig cellar for storing things mud at the bottom of the cellar for storing things of cellar for storing things mud not aging in the pond, cellar for storing things or appearance degeneration loose, to dig loose cellar for storing things mud weight as benchmark, add 5～15% above-mentioned mixed culture bacterium liquid and dig loose cellar for storing things mud mixing, Jiao Chi is applied flat with the cellar for storing things mud behind the mixing again.
Preferably, every a bite Jiao Chi processes 2～3 times as stated above.
Below by embodiment the specific embodiment of the present invention is further described, but therefore protection scope of the present invention is not limited among the embodiment.
Embodiment one
Select to produce Quality of Liquors identical and compare experiment with batch two mouthfuls of Xin Jiao ponds of building, wherein No. 1 Jiao Chi is according to the following step process of carrying out:
1, preparation and the treatment process of polynary foster cellar for storing things liquid:
(1) artificial eye-liquid is cultivated the methagen mixed solution that filters out, and adds yeast extract paste 1 ‰ at 35 ℃ of bacterium liquid namely for methane bacillus nutrient solutions of cultivating 30 days.
(2) artificial eye-liquid is cultivated to screen and is produced the well behaved caproic acid bacterial classification of caproic acid, add yeast extract paste 1 ‰, sodium-acetate 5 ‰, 95Vol% alcohol 2%, dipotassium hydrogen phosphate 0.25 ‰, pH is adjusted to 6.8, and the bacterium liquid that liquid anaerobism was cultivated 7 days under 35 ℃ of constant temperatures is the caproic acid bacteria nutrient solution.
(3) choose ripe quality aged pit mud and 40 ℃ cold boiling water, stir loose mixing according to the ratio of 1:1 and yeast extract paste 1 ‰, sodium-acetate 5 ‰, the dipotassium hydrogen phosphate 0.25 ‰ of interpolation old cellar for storing things mud and cold boiling water gross weight, pack into fermenting container and with alcohol to the sterilization of vessel port and liquid level, be the liquid old cellar for storing things of the present invention mud at 35 ℃ of lower bacterium liquid of cultivating 4 days.
(4) select female poor 4 weight parts of high-quality, high-quality yellow water 4 weight parts, middle high-temperature daqu powder 3 weight parts, yeast extract paste 0.1 weight part, sodium-acetate 0.2 weight part, dipotassium hydrogen phosphate 0.025 weight part, above-mentioned article have been added the boiling water of rear use 40 weight parts and heat-treated and closed container; Treat that temperature is down to 40 ℃ in the fermenting container, add methagen nutrient solution 12 weight parts, caproic acid bacteria nutrient solution 3 weight parts, liquid old cellar for storing things mud 20 weight parts, 95Vol% alcohol 2 weight parts, all the other fill up culture vessel with cold boiling water, transfer pH to 6.8 35 ℃ of lower cultivations 25 days, namely obtain mixed culture bacterium liquid.
(5) dig cellar for storing things mud at the bottom of the cellar for storing things of cellar for storing things mud not aging in the pond, No. 1 cellar for storing things or appearance degeneration loose with rake, add 200 kilograms of ripe mixed culture bacterium liquid (digging loose cellar for storing things mud weight 10%) mixing, wait to handle cellar for storing things mud is applied smooth for normal production, 2 rounds of continuous above-mentioned processing.
2, all carry out former cellar for storing things according to identical manufacturing parameter and technological operation and produce, the end, cellar for storing things grain unstrained spirits produces the wine quality and sees Table 1 and table 2:
The main physical and chemical index unit of table 1 wine body: mg/100g
The sensory evaluation of table 2 wine body
| Contrast Jiao Chi | Sensory evaluation |
| No. 1 Jiao Chi | The cellar for storing things perfume (or spice) stronger, sweet mellow, fragrance is coordinated, aftertaste is clean |
| Blank | Cellar for storing things perfume is not obvious, more pure and sweet, the wine body is full not, the aftertaste bitter taste is more obvious |
Embodiment two
Select to produce the Quality of Liquors two Kou Jiao ponds close with degree of degeneration and compare experiment.
1, preparation and the treatment process of polynary foster cellar for storing things liquid:
(1) artificial eye-liquid is cultivated the methagen mixed solution that filters out, and adds yeast extract paste 1 ‰ at 35 ℃ of bacterium liquid of cultivating 30 days, namely for methane bacillus nutrient solution.
(2) artificial eye-liquid is cultivated to screen and is produced the well behaved caproic acid bacterial classification of caproic acid, add yeast extract paste 1 ‰, sodium-acetate 5 ‰, 95Vol% alcohol 2%, dipotassium hydrogen phosphate 0.25 ‰, pH is adjusted to 6.8, and liquid anaerobism is cultivated 10 days bacterium liquid, i.e. caproic acid bacteria nutrient solution under 35 ℃ of constant temperatures.
(3) choose ripe quality aged pit mud and 40 ℃ cold boiling water, according to the ratio of 1:0.8 and add yeast extract paste 1 ‰, sodium-acetate 5 ‰, dipotassium hydrogen phosphate 0.25 ‰ and stir loose mixing, pack into fermenting container and with alcohol to the sterilization of vessel port and liquid level, at 35 ℃ of lower bacterium liquid of cultivating 4 days, i.e. the liquid old cellar for storing things of the present invention mud.
(4) select female poor 4 weight parts of high-quality, high-quality yellow water 4 weight parts, middle high-temperature daqu powder 3 weight parts, yeast extract paste 0.1 weight part, sodium-acetate 0.2 weight part, dipotassium hydrogen phosphate 0.025 weight part, above-mentioned article have been added the boiling water of rear use 40 weight parts and heat-treated and closed container; Treat that temperature is down to 40 ℃ in the fermenting container, add methagen nutrient solution 12 weight parts, caproic acid bacteria nutrient solution 5 weight parts, liquid old cellar for storing things mud 20 weight parts, 95Vol% alcohol 2 weight parts, all the other fill up culture vessel with cold boiling water, transfer pH to 6.8 35 ℃ of lower cultivations 30 days, namely obtain mixed culture bacterium liquid.
(5) cellar for storing things mud digs loosely at the bottom of cellar for storing things mud that will be not aging or the cellar for storing things that occurs degenerating with rake, adds 250 kilograms of ripe mixed culture bacterium liquid (digging loose cellar for storing things mud weight 10%) mixing, wait to handle with cellar for storing things mud deposited smooth for normal production, 3 rounds of continuous above-mentioned processing.
2, all carry out former cellar for storing things according to identical manufacturing parameter and technological operation and produce, the end, cellar for storing things grain unstrained spirits produces wine quality such as table 3 and table 4:
The main physical and chemical index unit of table 3 wine body: mg/100g
The sensory evaluation of table 4 wine body
| Contrast Jiao Chi | Sensory evaluation |
| Experiment Jiao Chi | Cellar for storing things perfume (or spice) obviously, more mellow, the smell coordination of wine body, aftertaste be clean |
| Contrast Jiao Chi | The cellar for storing things fragrant not obvious, owe pure and sweet, the wine body is thin, the aftertaste bitter taste is obvious |
Embodiment three
Each compares experiment flatly to select Xin Jiao pond and ripe Lao Jiao pond, and the Xin Jiao pond adopts the inventive method to process.
1, preparation and the treatment process of polynary foster cellar for storing things liquid:
(1) artificial eye-liquid is cultivated the methagen mixed solution that filters out, and adds yeast extract paste 1 ‰ at 35 ℃ of bacterium liquid of cultivating 30 days, namely for methane bacillus nutrient solution.
(2) artificial eye-liquid is cultivated to screen and is produced the well behaved caproic acid bacterial classification of caproic acid, add yeast extract paste 1 ‰, sodium-acetate 5 ‰, 95Vol% alcohol 2%, dipotassium hydrogen phosphate 0.25 ‰, pH is adjusted to 6.8, and liquid anaerobism is cultivated 10 days bacterium liquid, i.e. caproic acid bacteria nutrient solution under 35 ℃ of constant temperatures.
(3) choose ripe quality aged pit mud and 40 ℃ cold boiling water, according to the ratio of 1:1 and add yeast extract paste 1 ‰, sodium-acetate 5 ‰, dipotassium hydrogen phosphate 0.25 ‰ and stir loose mixing, pack into fermenting container and with alcohol to the sterilization of vessel port and liquid level, at 35 ℃ of lower bacterium liquid of cultivating 5 days, i.e. the liquid old cellar for storing things of the present invention mud.
(4) select female poor 4 weight parts of high-quality, high-quality yellow water 4 weight parts, middle high-temperature daqu powder 3 weight parts, yeast extract paste 0.1 weight part, sodium-acetate 0.2 weight part, dipotassium hydrogen phosphate 0.025 weight part, the boiling water that above-mentioned article has been added rear use 40 weight parts carries out heat place and closed container reason; Treat that temperature is down to 40 ℃ in the fermenting container, add methagen nutrient solution 12 weight parts, caproic acid bacteria nutrient solution 5 weight parts, liquid old cellar for storing things mud 20 weight parts, 95Vol% alcohol 2 weight parts, all the other fill up culture vessel with cold boiling water, transfer pH to 6.8 35 ℃ of lower cultivations 30 days, namely obtain mixed culture bacterium liquid.
(5) cellar for storing things mud digs loosely at the bottom of cellar for storing things mud that will be not aging or the cellar for storing things that occurs degenerating with rake, adds 250 kilograms of ripe mixed culture bacterium liquid (digging loose cellar for storing things mud weight 10%) mixing, wait to handle with cellar for storing things mud deposited smooth for normal production, 2 rounds of continuous above-mentioned processing.
2, all carry out former cellar for storing things according to identical manufacturing parameter and technological operation and produce, the end, cellar for storing things grain unstrained spirits produces wine quality such as table 5 and table 6:
The main physical and chemical index unit of table 5 wine body: mg/100g
The sensory evaluation of table 6 wine body
Through the analysis of above comparison example as can be known: example is one contrast result show, for the new cellar for storing things mud of same batch, present method agingly has an obvious promoter action to it, is mainly reflected in out the wine quality and significantly improves; Example two comparing results show, the maintenance that utilizes this mixed bacteria liquid to carry out, and to the phenomenon that cellar for storing things mud occurs hardening and degenerates, tool improves significantly, and to going out the wine quality obvious raising is arranged also simultaneously, and main manifestations is for take the increase of ethyl hexanoate as main body fragrant content; Example three comparing results show, the Xin Jiao pond that utilizes this mixed bacteria liquid maintenance to process, and it goes out the wine quality and stores out the wine quality near the old of natural maturity, has obvious promoter action therefore further verify the method to cellar for storing things mud is aging.
As known from the above examples: the maintenance by this mixed bacteria liquid is processed, and the microbial bacteria group structure of cellar for storing things mud is optimized, and cellar for storing things mud Oranoleptic indicator, physical and chemical index and microbiological indicator all improve significantly; Go out simultaneously the wine quality and obviously improve, liquor body style shows as: store aromatic strongly fragrant, the wine body is coordinated more, mellow.By the application of present method, newly storing mud can reach or near the effect of natural aging, improve significantly for pond, the cellar for storing things tool that occurs degenerating simultaneously in the short period.
Claims (10)
1. make fast aging mixed culture bacterium liquid of the new cellar for storing things of aromatic Chinese spirit, it is characterized in that comprising: the liquid old cellar for storing things mud of the methagen nutrient solution of 5～20 weight parts, the caproic acid bacteria nutrient solution of 1～7 weight part and 15～30 weight parts.
2. the fast aging mixed culture bacterium liquid of the new cellar for storing things of aromatic Chinese spirit that makes according to claim 1 is characterized in that comprising: the liquid old cellar for storing things mud of the methagen nutrient solution of 10～14 weight parts, the caproic acid bacteria nutrient solution of 3～5 weight parts and 18～25 weight parts.
3. the aromatic Chinese spirit that makes according to claim 1 and 2 is newly stored fast aging mixed culture bacterium liquid, it is characterized in that being made by following methods: female grain of 3～5 weight parts, the yellow water of 3～5 weight parts, the middle high-temperature daqu of 2～4 weight parts, the yeast extract paste of 0.05～0.2 weight part, the sodium-acetate of 0.1～0.3 weight part and the dipotassium hydrogen phosphate of 0.02～0.03 weight part are put into fermenting container, and the boiling water that adds again 30～50 weight parts carries out sterilising treatment and seals fermenting container; When the temperature in the fermenting container is down to 40 ℃, in container, add methagen nutrient solution, caproic acid bacteria nutrient solution, liquid old alcohol of storing mud and 1～3 weight part, fill up fermenting container with cold boiling water again and regulate pH value to 6.5～7.0, under 33～37 ℃ temperature, cultivated 20～30 days.
4. according to claim 1, the 2 or 3 described fast aging mixed culture bacterium liquid of the new cellar for storing things of aromatic Chinese spirits that make, it is characterized in that: described methagen nutrient solution is to add yeast extract paste in the methagen liquid that filters out cultivate bacterium liquid more than 30 days under 33～37 ℃ of temperature by cultivating to artificial eye-liquid.
5. the aromatic Chinese spirit that makes according to claim 4 is newly stored fast aging mixed culture bacterium liquid, and it is characterized in that: the weight of the yeast extract paste of interpolation is 0.5～1.5 ‰ of methagen liquid weight.
6. according to claim 1, the 2 or 3 described fast aging mixed culture bacterium liquid of the new cellar for storing things of aromatic Chinese spirit that make, it is characterized in that: described caproic acid bacteria nutrient solution is to add yeast extract paste, sodium-acetate, alcohol and dipotassium hydrogen phosphate by cultivating in the caproic acid bacterial classification that filters out to artificial eye-liquid, re-adjustment pH value to 6.5～7.0, liquid anaerobism is cultivated 7～10 days bacterium liquid under 35 ℃ of constant temperatures.
7. the aromatic Chinese spirit that makes according to claim 6 is newly stored fast aging mixed culture bacterium liquid, it is characterized in that: take the weight of caproic acid bacterial classification as benchmark, the weight of adding yeast extract paste is 0.5～1.5 ‰, the weight of sodium-acetate is 4～6 ‰, the weight of alcohol is 1～3%, the weight of dipotassium hydrogen phosphate is 0.2～0.3 ‰.
8. according to claim 1, the 2 or 3 described fast aging mixed culture bacterium liquid of the new cellar for storing things of aromatic Chinese spirit that make, it is characterized in that: it is with after 38～42 ℃ cold boiling water mixes by the weight ratio of 1 ︰ 0.8～1 with the old cellar for storing things mud of maturation that described liquid state is stored mud always, add again yeast extract paste, sodium-acetate and dipotassium hydrogen phosphate stirring and evenly mixing, pack into fermenting container and with alcohol to the sterilization of vessel port and liquid level, under 33～37 ℃ temperature, cultivate 3～5 days bacterium liquid.
9. the aromatic Chinese spirit that makes according to claim 8 is newly stored fast aging mixed culture bacterium liquid, it is characterized in that: take the gross weight of old cellar for storing things mud and cold boiling water as benchmark, the weight of adding yeast extract paste is 0.5～1.5 ‰, the weight of sodium-acetate is 4～6 ‰, the weight of dipotassium hydrogen phosphate is 0.2～0.3 ‰.
10. make aromatic Chinese spirit fast aging maintenance process of new cellar for storing things, it is characterized in that: will store cellar for storing things mud not aging in the pond or the cellar for storing things of occur degenerating at the bottom of cellar for storing things mud dig loose, to dig loose cellar for storing things mud weight as benchmark, add in 5～15% the claim 1～9 each described mixed culture bacterium liquid and dig loose cellar for storing things mud mixing, Jiao Chi is applied flat with the cellar for storing things mud behind the mixing again.
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| CN105385644A (en) * | 2015-12-29 | 2016-03-09 | 泸州品创科技有限公司 | Functional microbial agent for synthesizing caproic acid by degrading lactic acid and application thereof to pit mud curing |
| CN106085741A (en) * | 2016-08-30 | 2016-11-09 | 花冠集团酿酒股份有限公司 | A kind of aromatic Chinese spirit band pit mud nutrient fluid cultural method |
| CN106434125A (en) * | 2016-10-17 | 2017-02-22 | 湖北枝江酒业股份有限公司 | Wine making multi-bacteria functional bacterial liquid, and production method and application thereof |
| CN106479830A (en) * | 2016-12-19 | 2017-03-08 | 钦州市钦南区科学技术情报研究所 | A kind of Luzhou-flavor liquor pit mud curing fluid and preparation method thereof |
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| CN111334390A (en) * | 2020-04-28 | 2020-06-26 | 安徽古井贡酒股份有限公司 | Method for comprehensively improving quality of pit mud at bottom of strong aromatic Chinese spirits pool |
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| CN103695241B (en) * | 2013-12-11 | 2016-03-02 | 安徽龙舒酒业有限公司 | A kind of Biological organic liquor pit soil |
| CN103695241A (en) * | 2013-12-11 | 2014-04-02 | 安徽龙舒酒业有限公司 | Biological organic liquor pit soil |
| CN104087478A (en) * | 2014-07-21 | 2014-10-08 | 安徽古井贡酒股份有限公司 | Method for culturing bacterial solution used for inoculation of pit mud |
| CN104087478B (en) * | 2014-07-21 | 2016-04-20 | 安徽古井贡酒股份有限公司 | A kind of bacterium liquid cultural method for storing mud inoculation |
| CN105385644B (en) * | 2015-12-29 | 2019-02-12 | 泸州品创科技有限公司 | The functional microorganism microbial inoculum of degradation lactic acid synthesizing hexanoic acid and its application in pit mud maintenance |
| CN105385644A (en) * | 2015-12-29 | 2016-03-09 | 泸州品创科技有限公司 | Functional microbial agent for synthesizing caproic acid by degrading lactic acid and application thereof to pit mud curing |
| CN106085741A (en) * | 2016-08-30 | 2016-11-09 | 花冠集团酿酒股份有限公司 | A kind of aromatic Chinese spirit band pit mud nutrient fluid cultural method |
| CN106434125A (en) * | 2016-10-17 | 2017-02-22 | 湖北枝江酒业股份有限公司 | Wine making multi-bacteria functional bacterial liquid, and production method and application thereof |
| CN106479830A (en) * | 2016-12-19 | 2017-03-08 | 钦州市钦南区科学技术情报研究所 | A kind of Luzhou-flavor liquor pit mud curing fluid and preparation method thereof |
| CN107586643A (en) * | 2017-09-27 | 2018-01-16 | 安徽古井贡酒股份有限公司 | A kind of distiller's yeast culturing process for liquor production |
| CN110029076A (en) * | 2019-04-23 | 2019-07-19 | 中国科学院成都生物研究所 | A kind of compound caproic acid bacteria solution and preparation method thereof for producing Luzhou-flavor liquo |
| CN110777033A (en) * | 2019-12-03 | 2020-02-11 | 安徽迎驾贡酒股份有限公司 | Preparation method of culture solution for accelerating aging of new pit mud |
| CN110846168A (en) * | 2019-12-31 | 2020-02-28 | 河北凤来仪酒业有限公司 | Strong-aroma clean elegant white spirit pit mud and culture method thereof |
| CN110846168B (en) * | 2019-12-31 | 2023-01-13 | 河北凤来仪酒业有限公司 | Strong-aroma clean elegant white spirit pit mud and culture method thereof |
| CN111334390A (en) * | 2020-04-28 | 2020-06-26 | 安徽古井贡酒股份有限公司 | Method for comprehensively improving quality of pit mud at bottom of strong aromatic Chinese spirits pool |
| CN111334390B (en) * | 2020-04-28 | 2023-03-21 | 安徽古井贡酒股份有限公司 | Method for comprehensively improving quality of pit mud at bottom of strong aromatic Chinese spirits pool |
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