CN105385644B - The functional microorganism microbial inoculum of degradation lactic acid synthesizing hexanoic acid and its application in pit mud maintenance - Google Patents
The functional microorganism microbial inoculum of degradation lactic acid synthesizing hexanoic acid and its application in pit mud maintenance Download PDFInfo
- Publication number
- CN105385644B CN105385644B CN201511010000.0A CN201511010000A CN105385644B CN 105385644 B CN105385644 B CN 105385644B CN 201511010000 A CN201511010000 A CN 201511010000A CN 105385644 B CN105385644 B CN 105385644B
- Authority
- CN
- China
- Prior art keywords
- clostridium
- microbial inoculum
- pit mud
- functional microorganism
- pit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
Abstract
The present invention relates to it is a kind of with degradation the effect of lactic acid synthesizing hexanoic acid microorganism mix bacterium agent, the microbial inoculum include clostridium tyrobutyricum, clostridium sporogenes, clostridium klebsi viable bacteria, can be used for the maintenance of pit mud in white wine pit.Mixed bacterial of the invention can be used for pit mud maintenance, can reduce lactic acid accumulation in pit mud, avoid pit mud hardened, moreover it is possible to improve important flavor substance caproic acid and ethyl hexanoate content in white wine, produce good white wine.
Description
Technical field
The invention belongs to brewing technical fields, and in particular to a kind of to have the function of the micro- life of lactic acid synthesizing hexanoic acid effect of degrading
Object microbial inoculum, and application microbial bacterial agent maintenance pit, the method for delaying pit aging, improving out wine quality.
Background technique
The fermentation of mud cellar is the important process characteristic of Production of Luzhou-flavor Liquor, inhabites the micro- life of brewing function abundant in pit mud
Object, predominantly clostridium (such as caproic acid bacteria, butyric), purple monad, syntrophism monad, sarcina methanica and methane
Bacterium just forms just because of the raised growth of these functional microorganisms, breeding, metabolism and mutual synergistic effect with caproic acid
The typical style of ethyl ester, the Luzhou-flavor liquo that ethyl butyrate is main body note.Therefore pit mud quality has been largely fixed white wine
Quality, be influence Luzhou-flavor liquo quality key factor.
" storing out good wine always ", exactly because pit constantly tames in continuous fermentation process, is enriched with the micro- life of brewing function
Object, as pit are aging.But in the actual production process, there is also pit problem of aging, are mainly shown as pit mud water holding
Power decline, dries up hardened, and the needle-shaped or crystalline particulates such as white, green and red occurs in surface, has seriously affected the matter of white wine
Amount.The main reason for causing pit to degenerate is: one, entering to store grain grain moisture content, not enough, behind cellar exposed overlong time causes moisture
Volatilization causes pit mud moisture content to decline.Two, fermented grain acidity is excessive, causes lactic acid accumulation, the Ca in lactic acid and yellow mud, Fe ion
In conjunction with calcium lactate, ferric lactate precipitating is formed, these are deposited in pit mud and accumulate, and cause pit mud hardened.Three, pit mud is hardened and moisture
Deficiency affects transmitting of the grain grain nutriment in solid-state pit mud, causes the exhaustion of nutritional ingredient in pit mud, seriously affects micro-
Biological growth breeding and metabolic activity, cause functional microorganism to reduce, and are unfavorable for the product accumulation of microorganism in pit mud, accelerate cellar
Pond degeneration and wane.High-quality pit is the treasure and core competitiveness of Liquor-making Enterprises &, therefore establishes a perfect pit and support
Maintaining method reaches and avoids pit aging, and the purpose for accelerating pit aging becomes the important task of Liquor-making Enterprises &.
Summary of the invention
It can increase caproic acid content in pit mud technical problem to be solved by the invention is to provide a kind of, reduce lactic acid content
Functional microorganism microbial inoculum.
The functional microorganism microbial inoculum of present invention degradation lactic acid synthesizing hexanoic acid, including clostridium tyrobutyricum, clostridium sporogenes and kirschner
Clostridium, and the number of viable ratio of clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi is 0.8~1.2:1:1.2~1.5.
Wherein, above-mentioned functional microorganism microbial inoculum is liquid bacterial agent, and wherein the concentration of bacterium is 106~1010CFU/mL。
Wherein, above-mentioned functional microorganism microbial inoculum is that clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi are inoculated in half respectively
The microbial inoculum that the culture solution for obtain after Anaerobic culturel in synthesis yellow water culture medium is mixed to get.Wherein, above-mentioned functional microorganism
The formula of semi-synthetic yellow water culture medium in microbial inoculum are as follows: 4~6g/L of sodium acetate, 0.2~0.8g/L of ammonium sulfate, dipotassium hydrogen phosphate
0.2~0.6g/L, 0.1~0.3g/L of magnesium sulfate, 0.5~1.5g/L of yeast extract, 0.3~0.7g/L of cysteine hydrochloride, water
Respectively account for half by volume with yellow water, adjusting pH value is 5.5~6.5, is sterilized to obtain the final product.
Wherein, clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi described in above-mentioned functional microorganism microbial inoculum are inoculated with respectively
Inoculum concentration 5~10%；33~37 DEG C of the temperature of the anaerobic fermentation, Anaerobic culturel 5~7 days.The mixing of culture solution is generally pressed
Ratio mixing.
The present invention also provides application of the above-mentioned functional microorganism microbial inoculum in the maintenance of brewed spirit pit mud.
Furthermore the present invention also provides a kind of methods using above-mentioned energy microbial bacterial agent maintenance brewed spirit pit mud.It should
Method the following steps are included:
A, above-mentioned functional microorganism bacterium, the yellow water dilution of pH 4.0~6.0 and koji powder are mixed and made into paste cellar
Mud curing agent；
B, after cellar is played in wine cellar, paste pit mud curing agent is equably applied in Jiao Bi and cellar bottom surface, next batch grain grain is just
Normal pit entry fermentation.
Wherein, the yellow water dilution being added in above method a step is yellow water and water isometric to be mixed as what 1:1 was prepared
Liquid.
Wherein, in the above method a step functional microorganism flora, the yellow water dilution of pH 4.0~6.0, koji powder matter
Amount is than being 1 ︰, 0.8~1.2 ︰ 1.5~2.0.
Wherein, in the above method b step pit mud curing agent smearing thickness in 0.5~1.5cm.
The beneficial effects of the present invention are: the present invention has the microorganism mixed bacterial use of degradation lactic acid synthesizing hexanoic acid effect
It is conserved in pit mud, lactic acid content in pit mud can be significantly reduced, hence it is evident that promoted next round and ferment wine quality out.The junket that the present invention uses
Without apparent antagonism between each strain such as clostridium butyricum, clostridium sporogenes and clostridium klebsi, it can be good at cooperation and play work
With.Pit is conserved using the method for the present invention, the accumulation of lactic acid in pit mud can be significantly reduced, increases caproic acid content, avoids pit
Aging.The present invention provides a kind of new selection for pit maintenance, has broad application prospects.
Specific embodiment
A specific embodiment of the invention is described further below with reference to embodiment, is not therefore limited the invention to
In the embodiment described range.
The functional microorganism microbial inoculum of present invention degradation lactic acid synthesizing hexanoic acid, including clostridium tyrobutyricum, clostridium sporogenes and kirschner
Clostridium, and the number of viable ratio of clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi is 0.8~1.2:1:1.2~1.5.It is above-mentioned
Functional microorganism microbial inoculum can be prepared into solid or for liquid bacterial agent, when product form is liquid bacterial agent, wherein bacterium
Concentration is 106~1010CFU/mL。
Further, above-mentioned functional microorganism microbial inoculum is to be inoculated with clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi respectively
The bacteria liquid agent that the culture solution for carrying out obtaining after Anaerobic culturel in semi-synthetic yellow water culture medium is mixed to get.Wherein, above-mentioned function
The formula of semi-synthetic yellow water culture medium in energy microbial bacterial agent are as follows: 4~6g/L of sodium acetate, 0.2~0.8g/L of ammonium sulfate, phosphoric acid
0.2~0.6g/L of hydrogen dipotassium, 0.1~0.3g/L of magnesium sulfate, 0.5~1.5g/L of yeast extract, cysteine hydrochloride 0.3~
0.7g/L, water and yellow water respectively account for half by volume, and adjusting pH value is 5.5~6.5, sterilize to obtain the final product.Preferably, above-mentioned semi-synthetic Huang
The formula of water culture medium are as follows: sodium acetate 5g/L, ammonium sulfate 0.5g/L, dipotassium hydrogen phosphate 0.4g/L, magnesium sulfate 0.2g/L, yeast extract
1g/L, cysteine hydrochloride 0.5g/L, water and yellow water respectively account for half by volume, and adjusting pH value is 6.0, sterilize to obtain the final product.
Wherein, clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi described in above-mentioned functional microorganism microbial inoculum are inoculated with respectively
Inoculum concentration 5~10%；33~37 DEG C of the temperature of the anaerobic fermentation, Anaerobic culturel 5~7 days.The mixing of culture solution is generally pressed
Ratio mixing.
The above-mentioned functional microorganism microbial inoculum of the present invention can be used in the maintenance of brewed spirit pit mud.Gather the pit mud maintenance of justice
Method the following steps are included:
A, above-mentioned functional microorganism microbial inoculum amount, the yellow water dilution of pH5.0 and koji powder are mixed and made into paste pit mud
Curing agent；
B, after cellar is played in wine cellar, paste pit mud curing agent is equably applied in Jiao Bi and cellar bottom surface, next batch grain grain is just
Normal pit entry fermentation.
Wherein, the yellow water dilution being added in above method a step is yellow water and water isometric to be mixed as what 1:1 was prepared
Liquid.
Wherein, in the above method a step functional microorganism flora, the yellow water dilution of pH 4.0~6.0, koji powder matter
Amount is than being 1 ︰, 0.8~1.2 ︰ 1.5~2.0.The pH value of yellow water dilution is preferably 5.0.Pit mud curing agent smearing thickness in b step
More it is suitable in 0.5~1.5cm.
Major function bacterium bag includes clostridium tyrobutyricum (Clostridium in microorganism mix bacterium agent provided by the invention
Tyrobutyricum), clostridium sporogenes (Clostridium sporogenes) and clostridium klebsi (Clostridium
Kluyveri) three kinds of bacteriums.Clostridium butyricum and clostridium sporogenes energy efficient degradation lactic acid, clostridium klebsi can produce caproic acid using lactic acid.
Three does not have apparent antagonism, and can be good at cooperation and play a role, and microbial inoculum of the present invention is made on this basis.
Following embodiment has the functional microorganism flora of degradation lactic acid synthesizing hexanoic acid effect using the method for the present invention preparation
Pit mud curing agent, and be used for pit routine servicing, to verify its effect.
The preparation of embodiment one, microbial inoculum of the present invention
Take clostridium tyrobutyricum (Clostridium tyrobutyricum, deposit number: ATCC 25755), clostridium sporogenes
(Clostridium kluyveri is protected for (Clostridium sporogenes, deposit number: ATCC 11437) and clostridium klebsi
Hiding number: ATCC 8527) fermenting in 20L fermentor respectively, and culture medium is semi-synthetic yellow water culture medium, liquid amount 10L, inoculation
Amount 10%, 35 DEG C of temperature, anaerobic fermentation 7 days.
Clostridium tyrobutyricum (Clostridium tyrobutyricum, deposit number: ATCC 25755), clostridium sporogenes
(Clostridium kluyveri is protected for (Clostridium sporogenes, deposit number: ATCC 11437) and clostridium klebsi
Hiding number: ATCC 8527) respectively from streak inoculation in cryopreservation tube into semi-synthetic yellow water agar medium (sodium acetate 5g/L, sulfuric acid
Ammonium 0.5g/L, dipotassium hydrogen phosphate 0.4g/L, magnesium sulfate 0.2g/L, yeast extract 1g/L, cysteine hydrochloride 0.5g/L, agar powder
20g/L, water and yellow water respectively account for half, pH value 6.0 by volume), it is cultivated 5 days for 35 DEG C in anaerobic culture box.Then picking
Single colonie is inoculated into (sodium acetate 5g/L, ammonium sulfate 0.5g/L, dipotassium hydrogen phosphate 0.4g/ in the semi-synthetic yellow water culture medium of 1L respectively
L, magnesium sulfate 0.2g/L, yeast extract 1g/L, cysteine hydrochloride 0.5g/L, water and yellow water respectively account for half, pH value by volume
6.0), to cultivate 5 days for 35 DEG C in anaerobic culture box, being then seeded into 20L fermentor and ferment respectively, culture medium is semi-synthetic
Yellow water culture medium, liquid amount 10L, inoculum concentration 10%, 35 DEG C of temperature, anaerobic fermentation 7 days.Plate count result is clostridium tyrobutyricum
Fermentation liquid 5.5 × 108CFU/ml, clostridium sporogenes fermentation liquid 4.3 × 108CFU/ml, clostridium klebsi fermentation liquid 7.9 × 107CFU/
ml.Three kinds of fermentation liquids are mixed according to volume ratio 1:1:1.5 to get functional microorganism group fermentation liquid.
Embodiment two carries out pit mud maintenance using microbial inoculum of the present invention
Functional microorganism group fermentation liquid made from Example 1, pH value 5.0 yellow water and water the isometric mixed liquor of 1:1,
Koji powder (Luzhou Huai Yu koji-making Co., Ltd, long perfume board yeast) is uniformly mixed according to the ratio of mass ratio 1:1:1.8, is made
Paste pit mud curing agent 115kg, moisture content about 60%.
After playing cellar, paste pit mud curing agent is equably applied in Jiao Bi and cellar bottom, thickness about 1cm.Next batch grain grain is just
Often enter cellar and carries out fermenting and producing Luzhou-flavor liquo.
The result shows that the pit and conventional method using the method for the present invention maintenance conserve (spreading starter powder, yellow water and tail wine)
Pit is compared, and under same raw material and technique working condition, is produced Luzhou-flavor liquo, is compared main body fragrance matter in white wine and contain
Amount, the results are shown in Table 1.
1 pit of table goes out main body fragrance content of material (unit: g/L) in wine
| Ethyl hexanoate | Ethyl butyrate | Ethyl acetate | Caproic acid | Butyric acid | Acetic acid | |
| The method of the present invention | 3.35 | 0.45 | 1.97 | 0.98 | 0.19 | 0.38 |
| The feeding cellar method of tradition | 2.74 | 0.36 | 1.83 | 0.65 | 0.18 | 0.43 |
As can be seen from Table 1, in the Luzhou-flavor liquo of the pit brewing conserved using the present invention in main body fragrance matter oneself
The content of acetoacetic ester, ethyl butyrate and caproic acid is higher than the wine out that conventional method supports cellar pit, and it is white to illustrate that the present invention can be promoted effectively
The quality of wine.
Pit mud is taken after playing cellar, the lactate content in two mouthfuls of pit pit muds is measured using bio-sensing instrument, as a result such as 2 institute of table
Show.
Lactate content (unit: the dry mud of mg/100g) in 2 pit mud of table
| The method of the present invention | The feeding cellar method of tradition | |
| Lactate | 674.0 | 763.5 |
It is supported as can be seen from Table 2 using lactate content in the pit of the invention conserved after fermentation pit mud lower than tradition
The pit mud at cellar illustrates that the functional microorganism flora in the present invention can reduce the content of lactate in pit mud, effectively avoids pit mud
Hardened, aging.
Claims (8)
1. degrade lactic acid synthesizing hexanoic acid functional microorganism microbial inoculum, it is characterised in that: including clostridium tyrobutyricum, clostridium sporogenes and gram
Family name clostridium, and the number of viable ratio of clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi is 0.8~1.2:1:1.2~1.5；Institute
The functional microorganism microbial inoculum stated is that clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi are inoculated in semi-synthetic yellow water culture medium respectively
The microbial inoculum that the culture solution obtained after middle carry out Anaerobic culturel is mixed to get；Clostridium tyrobutyricum (the Clostridium
Tyrobutyricum) deposit number is ATCC 25755；Clostridium sporogenes (Clostridium sporogenes) deposit number is ATCC
11437；Clostridium klebsi (Clostridium kluyveri) deposit number is ATCC 8527.
2. functional microorganism microbial inoculum as described in claim 1, which is characterized in that the functional microorganism microbial inoculum is liquid bacteria
Agent, wherein the concentration of bacterium is 106~1010CFU/mL。
3. the functional microorganism microbial inoculum for lactic acid synthesizing hexanoic acid of degrading according to claim 1, it is characterised in that:
The formula of the semi-synthetic yellow water culture medium are as follows: 4~6g/L of sodium acetate, 0.2~0.8g/L of ammonium sulfate, dipotassium hydrogen phosphate
0.2~0.6g/L, 0.1~0.3g/L of magnesium sulfate, 0.5~1.5g/L of yeast extract, 0.3~0.7g/L of cysteine hydrochloride, water
Respectively account for half by volume with yellow water, adjusting pH value is 5.5~6.5, is sterilized to obtain the final product.
4. the functional microorganism microbial inoculum for lactic acid synthesizing hexanoic acid of degrading according to claim 1, it is characterised in that:
The inoculum concentration 5~10% that the clostridium tyrobutyricum, clostridium sporogenes and clostridium klebsi are inoculated with respectively；The temperature of the anaerobic fermentation
33~37 DEG C, Anaerobic culturel 5~7 days of degree；The mixing of culture solution is mixed by equal proportion.
5. application of the described in any item functional microorganism microbial inoculums of Claims 1 to 4 in the maintenance of brewed spirit pit mud.
6. the method for conserving brewed spirit pit mud, it is characterised in that the following steps are included:
A, by the described in any item functional microorganism bacterium of Claims 1 to 4, the yellow water dilution and koji powder of pH 4.0~6.0
It is mixed and made into paste pit mud curing agent；
B, after cellar is played in wine cellar, paste pit mud curing agent is equably applied in Jiao Bi and cellar bottom surface, next batch grain grain normally enters
Cellar fermentation.
7. method according to claim 6, it is characterised in that: the Huang of functional microorganism microbial inoculum, pH 4.0~6.0 in a step
Water diluent, koji powder mass ratio be 1 ︰, 0.8~1.2 ︰ 1.5~2.0.
8. method according to claim 6, it is characterised in that: pit mud curing agent smearing thickness is in 0.5~1.5cm in b step.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511010000.0A CN105385644B (en) | 2015-12-29 | 2015-12-29 | The functional microorganism microbial inoculum of degradation lactic acid synthesizing hexanoic acid and its application in pit mud maintenance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511010000.0A CN105385644B (en) | 2015-12-29 | 2015-12-29 | The functional microorganism microbial inoculum of degradation lactic acid synthesizing hexanoic acid and its application in pit mud maintenance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105385644A CN105385644A (en) | 2016-03-09 |
| CN105385644B true CN105385644B (en) | 2019-02-12 |
Family
ID=55418429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511010000.0A Active CN105385644B (en) | 2015-12-29 | 2015-12-29 | The functional microorganism microbial inoculum of degradation lactic acid synthesizing hexanoic acid and its application in pit mud maintenance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105385644B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834177B (en) * | 2017-02-21 | 2019-11-08 | 中国科学院成都生物研究所 | One plant of cud bacterium and its application |
| CN108690772A (en) * | 2018-06-29 | 2018-10-23 | 安徽瑞思威尔科技有限公司 | A kind of preparation method improving the mixed bacteria preparation of ethyl hexanoate content in aromatic Chinese spirit solid ferment process |
| CN109536331B (en) * | 2019-01-10 | 2022-04-01 | 四川大学 | Method for increasing and reducing milk in pit mud |
| CN111394397A (en) * | 2020-03-17 | 2020-07-10 | 北京科技大学 | Method for producing caproic acid by fermenting kitchen waste |
| CN111440838A (en) * | 2020-03-25 | 2020-07-24 | 北京科技大学 | Method for producing hexanoic acid by fermenting white spirit vinasse by enriching hexanoic acid functional bacteria in pit mud |
| CN114933996B (en) * | 2022-06-08 | 2023-10-27 | 四川剑南春（集团）有限责任公司 | Pit mud synthesis microbiome and preparation and application thereof |
| CN115232772A (en) * | 2022-08-04 | 2022-10-25 | 湖北稻花香酒业股份有限公司 | Method for producing caproic acid by using RF1 bacteria and combined clostridium keshiii |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174355A (en) * | 2011-02-25 | 2011-09-07 | 泸州品创科技有限公司 | Concentrated fragrance type distilled spirit pit mud pit-maintaining liquid, production method thereof and concentrated fragrance type distilled spirit pit mud maintenance method |
| CN102260640A (en) * | 2011-07-12 | 2011-11-30 | 四川省绵阳市丰谷酒业有限责任公司 | Hexanoic acid bacterium and liquid pig mud bacterium mixed bacterial solution culture method |
| CN103305448A (en) * | 2013-07-04 | 2013-09-18 | 四川省古川酒业有限公司 | Mixed culture bacterial solution for quickly aging new pit of strong aromatic Chinese spirits and maintenance method |
| CN103525743A (en) * | 2013-10-30 | 2014-01-22 | 四川绵竹剑南春酒厂有限公司 | Clostridium sporogenes and purpose thereof |
| CN104673693A (en) * | 2013-11-28 | 2015-06-03 | 鼎唐能源科技股份有限公司 | Acidogenic clostridium difficile and method for producing volatile fatty acids by using acidogenic clostridium difficile |
-
2015
- 2015-12-29 CN CN201511010000.0A patent/CN105385644B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174355A (en) * | 2011-02-25 | 2011-09-07 | 泸州品创科技有限公司 | Concentrated fragrance type distilled spirit pit mud pit-maintaining liquid, production method thereof and concentrated fragrance type distilled spirit pit mud maintenance method |
| CN102260640A (en) * | 2011-07-12 | 2011-11-30 | 四川省绵阳市丰谷酒业有限责任公司 | Hexanoic acid bacterium and liquid pig mud bacterium mixed bacterial solution culture method |
| CN103305448A (en) * | 2013-07-04 | 2013-09-18 | 四川省古川酒业有限公司 | Mixed culture bacterial solution for quickly aging new pit of strong aromatic Chinese spirits and maintenance method |
| CN103525743A (en) * | 2013-10-30 | 2014-01-22 | 四川绵竹剑南春酒厂有限公司 | Clostridium sporogenes and purpose thereof |
| CN104673693A (en) * | 2013-11-28 | 2015-06-03 | 鼎唐能源科技股份有限公司 | Acidogenic clostridium difficile and method for producing volatile fatty acids by using acidogenic clostridium difficile |
Non-Patent Citations (6)
| Title |
|---|
| 人工窖泥的研究进展;李海峰等;《酿酒》;20120331;第39卷(第2期);96-99 |
| 人工老窖泥培养中功能菌发酵液的制备;徐军等;《酿酒科技》;20111231(第10期);70-71 |
| 基于PCR-DGGE的浓香型白酒窖泥细菌群落结构研究;黄治国等;《西南大学学报》;20140831;第36卷(第8期);1-7 |
| 己酸菌、窖泥与浓香型白酒之间的关系;唐瑞;《酿酒》;20050731;第32卷(第4期);24-27 |
| 液体窖泥培养过程微生物和香气成份变化趋势;姚万春等;《中国酿造》;20131231;第32卷(第2期);45-48 |
| 窖泥老化与防治;孙景明;《酿酒》;20011130;第28卷(第6期);42-44 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105385644A (en) | 2016-03-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105385644B (en) | The functional microorganism microbial inoculum of degradation lactic acid synthesizing hexanoic acid and its application in pit mud maintenance | |
| CN102229874B (en) | Production method of Luzhou-flavor liquor | |
| CN102160595B (en) | Preparation process of complex microorganism fermented active feed | |
| CN104152318B (en) | A kind of artificial old brewing mud and preparation technology thereof | |
| CN105462872A (en) | Composite microecological preparation and preparation method thereof | |
| CN102260638B (en) | Pit mud functional bacteria and preparation method thereof | |
| CN103305448A (en) | Mixed culture bacterial solution for quickly aging new pit of strong aromatic Chinese spirits and maintenance method | |
| CN109666599B (en) | Lactobacillus reuteri high-density fermentation medium, fermentation method and application | |
| CN101280272A (en) | Microbial preparation, preparation and use thereof | |
| CN102286322B (en) | Composite microbial enzyme preparation and preparation method thereof | |
| CN112006066A (en) | Mixed fermentation liquor with improved antibacterial activity and preparation method and application thereof | |
| CN107853452A (en) | A kind of production method of additive for microbe feedstuff | |
| CN101451107B (en) | Method for large scale preparing Gliocladium chlamydospore | |
| CN113046253B (en) | Culture method for improving heat resistance of kluyveromyces marxianus | |
| CN102146325B (en) | Method for culturing closed cellar mud | |
| CN104513752A (en) | Method for curing pit mud | |
| CN101781631B (en) | Lactate lowering bacteria and culture process and application thereof in white spirit production technology | |
| CN110628543A (en) | Fermentation method for improving ratio of ethyl caproate to ethyl acetate of Luzhou-flavor liquor | |
| CN111961620B (en) | Compound microbial agent and application thereof in regulating pH of pit mud | |
| CN110150505B (en) | Lactic acid bacteria complexing agent for removing earthy smell of eriocheir sinensis and preparation method thereof | |
| CN103897920A (en) | Preparation method of space caproic acid bacteria pit mud for brewing wine | |
| KR20140020470A (en) | Method for producing fermented herb extract with high gaba content using lactobacillus helveticus rmk85 | |
| US20080213826A1 (en) | Compound microbial preparation manufacturing process | |
| CN110982660B (en) | Preparation method of fermented grains for solid-state acetic acid fermentation of mature vinegar | |
| CN104830736A (en) | Pediococcus pentosaceus strain and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |