CN105724550B - A method of carrying out fish scrap bone and flesh separation using microbial fermentation - Google Patents
A method of carrying out fish scrap bone and flesh separation using microbial fermentation Download PDFInfo
- Publication number
- CN105724550B CN105724550B CN201610129692.9A CN201610129692A CN105724550B CN 105724550 B CN105724550 B CN 105724550B CN 201610129692 A CN201610129692 A CN 201610129692A CN 105724550 B CN105724550 B CN 105724550B
- Authority
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- China
- Prior art keywords
- fish
- bacillus licheniformis
- fermentation
- bone
- flesh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 47
- 238000000855 fermentation Methods 0.000 title claims abstract description 47
- 230000004151 fermentation Effects 0.000 title claims abstract description 47
- 238000000926 separation method Methods 0.000 title claims abstract description 29
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000000813 microbial effect Effects 0.000 title claims abstract description 14
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- 102000035195 Peptidases Human genes 0.000 claims abstract description 5
- 235000019833 protease Nutrition 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 4
- 235000021190 leftovers Nutrition 0.000 claims description 17
- 239000011148 porous material Substances 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 claims description 2
- 241000252233 Cyprinus carpio Species 0.000 claims description 2
- 241000276435 Gadus Species 0.000 claims description 2
- 241000273924 Harengula Species 0.000 claims description 2
- 241000252234 Hypophthalmichthys nobilis Species 0.000 claims description 2
- 241001275898 Mylopharyngodon piceus Species 0.000 claims description 2
- 241000276707 Tilapia Species 0.000 claims description 2
- 230000005540 biological transmission Effects 0.000 claims description 2
- 235000013372 meat Nutrition 0.000 claims 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 235000019733 Fish meal Nutrition 0.000 description 4
- 239000004467 fishmeal Substances 0.000 description 4
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 235000019419 proteases Nutrition 0.000 description 3
- 238000010297 mechanical methods and process Methods 0.000 description 2
- 241000276701 Oreochromis mossambicus Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
- A22C—PROCESSING MEAT, POULTRY, OR FISH
- A22C25/00—Processing fish ; Curing of fish; Stunning of fish by electric current; Investigating fish by optical means
- A22C25/16—Removing fish-bones; Filleting fish
Abstract
The invention discloses a kind of methods carrying out fish scrap bone and flesh separation using microbial fermentation, including following key step：It is 1 in mass ratio by fresh fish scrap and water：1~3 ratio is added in fermentation vat；And the bacillus licheniformis of thermostable proteinase will be produced（Bacillus licheniformis）HL 3, which is linked into fermentation vat, to ferment；It is 55 DEG C~60 DEG C to control fermentation temperature, and fermentation time is 6h~12h；Material first passes through large aperture wire-mesh screen and is filtered separation retention fish-bone in fermentation vat after fermentation, is then filtered separation by small-bore wire-mesh screen through object by retains the flesh of fish.The bone and flesh separation method of fish scrap of the present invention has the characteristics that easy to operate and at low cost, there is good application prospect.
Description
Technical field
The present invention relates to a kind of methods carrying out fish scrap bone and flesh separation using microbial fermentation, belong to aquatic products and add
Work and the technical field utilized.
Background technology
The processing of the current fish in China is based on preliminary working frozen fillet.In the process of fillet, it will produce a large amount of
Fish scrap.These fish scraps are mainly used to be processed into fish meal at present, but before fish scrap processing fish meal
Bone and flesh is not detached, so that the protein content of the fish meal prepared is low, and fish-bone this resource does not also obtain
It utilizes well, therefore a kind of simple and practicable method of urgent need detaches the bone and flesh of fish scrap.
The method of fish scrap bone and flesh separation at present mainly has enzymatic isolation method and mechanical phonograph recorder separation.Enzymatic isolation method mainly adds
Protease hydrolyzed so that the bone and flesh in fish scrap reaches separation.But enzymatic isolation method needs to add expensive protease, cost
Height, it is difficult to large-scale utilization and extention.Mechanical Method is mainly detached fish-bone with the flesh of fish using deboner, but mechanical
Method need to put into expensive equipment, treating capacity is few, it is difficult to utilize on a large scale.
Invention content
For the problems of current fish scrap bone and flesh separation, the object of the present invention is to provide a kind of simple and practicable
Fish scrap bone and flesh separation method.
To solve the above-mentioned problems, the technical solution adopted in the present invention is as follows：It is a kind of to carry out fish using microbial fermentation
The method of class leftover bits and pieces bone and flesh separation, includes the following steps：
（1）By the bacillus licheniformis that can produce thermostable proteinase of slant preservation（Bacillus licheniformis）HL-3 is linked into the LB liquid medium of sterilizing postcooling, and 12h, system are cultivated in 55 DEG C, 150r/min
For at bacillus licheniformis（Bacillus licheniformis）HL-3 seed liquors are spare；
（2）It is 1 in mass ratio by fresh fish scrap and water：1 ~ 3 ratio is added in fermentation vat；
（3）Toward step（2）In equipped with fish scrap fermentation vat in access step（1）Prepared bacillus licheniformis
（Bacillus licheniformis）HL-3 seed liquors, and mixing；
（4）By step（3）The fish scrap of the addition bacterium of acquisition ferments in following manner：Fermentation temperature controls
At 55 ~ 60 DEG C, water surface elevation is 50cm~100cm in fermentation vat, and fermentation time is 6h~12h；
（5）After fermentation, the material in fermentation vat is filtered separation, trapped substance by large aperture metallic screen first
It is the flesh of fish and water through object for fish-bone；
（6）By step（5）The flesh of fish and water of transmission are filtered separation, trapped substance by the metallic screen of a small-bore again
For the flesh of fish.
The step（1）Used in can produce the bacillus licheniformis of thermostable proteinase（Bacillus licheniformis）HL-3 is preserved in Guangdong Province's Culture Collection on January 18th, 2016, and deposit number is
GDMCC No：60003, preservation address is 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100 Guangdong Microbes Inst.
The step（2）In fish scrap be leftovers of tilapia, gadus leftover bits and pieces, carp leftover bits and pieces, Harengula zunasi
One kind in leftover bits and pieces, black carp leftover bits and pieces, silver carp leftover bits and pieces, grass carp leftover bits and pieces and stripped tuna leftover bits and pieces.
The step（3）Middle bacillus licheniformis（Bacillus licheniformis）The inoculum concentration of HL-3 seed liquors with
The quality of access seed liquor accounts for fish scrap gross mass percentages, is 0.01%~5%.
The step（5）2 ~ 15cm of sieve pore of large pore metallic screen.The step（6）The sieve pore of small aperture metallic screen
0.01~2cm。
The present patent application compared with prior art, has the following advantages.
（1）It is at low cost.Microbial fermentation temperature is consistent with fish scrap endogenous enzymes peak enzymolysis-ability temperature in this technology, makes
Microbial fermentation is obtained together with the enzymolysis perfect adaptation of endogenous enzymes.Just because of the endogenous enzymes of the fish scrap made full use of
Enzymolysis so that the slight fermentation of bacillus licheniformis can reach the purpose of bone and flesh separation.With existing enzyme process bone and flesh
Separation is compared, and this technology does not have to separately add protease, at low cost.
（2）It is easy to operate.With mechanically decoupled method, this technology does not have to complicated equipment, in a simple fermentation vat
In.Also, this technology can not be grasped manually using a kind of slight fermentation without artificial ventilation, entire fermentation process
Make, it is simple and easy to control.
（3）Safety.This technology is using high temperature resistant bacillus licheniformis（Bacillus licheniformis）It carries out
Fermentation can inhibit the growth of pathogenic bacteria and spoilage organisms, thus technical solution provided by the invention safety at high temperature.
The present invention provides a kind of methods carrying out fish scrap bone and flesh separation using microbial fermentation.The present invention provides
Method have the characteristics that easy to operate and at low cost, have a good application prospect.Technology carries out under fish through the invention
After the bone and flesh separation of heel, the protein content of made fish meal can be not only improved, and can also be recycled to fish-bone.
Specific implementation mode
For a better understanding of the present invention, the selection below with regard to fish scrap, fermentation parameter and fermentation method combines in fact
Applying example, the invention will be further described, and but the scope of the present invention is not limited thereto.
Embodiment 1：By the bacillus licheniformis that can produce thermostable proteinase of slant preservation（Bacillus licheniformis）HL-3 is linked into the LB liquid medium of sterilizing postcooling, and 12h, system are cultivated in 55 DEG C, 150r/min
For at bacillus licheniformis（Bacillus licheniformis）HL-3 seed liquors are spare.By 1000 kilograms of fresh Tilapia mossambicas
Leftover bits and pieces and 2500 kg of water are added in fermentation vat, and water surface elevation is 60cm in fermentation vat；By 2 kilograms of bacillus licheniformis
（Bacillus licheniformis）HL-3 seed liquors are added in fermentation vat, and are stirred evenly；It is 55 DEG C to control fermentation temperature, hair
The ferment time is 9h；After fermentation, material in fermentation vat is filtered separation by the metallic screen that aperture is 12 cm, retained
Object is fish-bone, through the mixture that object is the flesh of fish and water；The flesh of fish and water that will transmit through are again the metal of 1 cm by an aperture
Sieve is filtered separation, and trapped substance is the flesh of fish.
Claims (6)
1. a kind of method carrying out fish scrap bone and flesh separation using microbial fermentation, it is characterised in that：This method includes such as
Lower step：
（1）By the bacillus licheniformis that can produce thermostable proteinase of slant preservation（Bacillus licheniformis）HL-3
It is linked into the LB liquid medium of sterilizing postcooling, cultivates 12h in 55 DEG C, 150r/min, be prepared into bacillus licheniformis
（Bacillus licheniformis）HL-3 seed liquors are spare；
（2）It is 1 in mass ratio by fresh fish scrap and water：1 ~ 2.5 ratio is added in fermentation vat；
（3）Toward step（2）In equipped with fish scrap fermentation vat in access step（1）Prepared bacillus licheniformis
（Bacillus licheniformis）HL-3 seed liquors, and mixing；
（4）By step（3）The fish scrap of the addition bacterium of acquisition ferments in following manner：Fermentation temperature is controlled 55
~ 60 DEG C, water surface elevation is 50cm~100cm in fermentation vat, and fermentation time is 6h~12h；
（5）After fermentation, the material in fermentation vat is filtered separation by large aperture metallic screen first, trapped substance is fish
Bone is the flesh of fish and water through object；
（6）By step（5）The flesh of fish and water of transmission are filtered separation by the metallic screen of a small-bore again, and trapped substance is fish
Meat.
2. a kind of method carrying out fish scrap bone and flesh separation using microbial fermentation according to claim 1, special
Sign exists
In：The step（1）Used in bacillus licheniformis（Bacillus licheniformis）HL-3 is micro- in Guangdong Province
The deposit number of biological inoculum collection is GDMCC No：60003.
3. a kind of method carrying out fish scrap bone and flesh separation using microbial fermentation according to claim 1, special
Sign exists
In：The step（2）In fish scrap be leftovers of tilapia, gadus leftover bits and pieces, carp leftover bits and pieces, under Harengula zunasi
One kind in heel, black carp leftover bits and pieces, silver carp leftover bits and pieces, grass carp leftover bits and pieces and stripped tuna leftover bits and pieces.
4. a kind of method carrying out fish scrap bone and flesh separation using microbial fermentation according to claim 1, special
Sign exists
In：The step（3）Middle bacillus licheniformis（Bacillus licheniformis）The inoculum concentration of HL-3 seed liquors with
The quality of access seed liquor accounts for fish scrap gross mass percentages, is 0.01%~5%.
5. a kind of method carrying out fish scrap bone and flesh separation using microbial fermentation according to claim 1, special
Sign exists
In：The step（5）The sieve pore of large pore metallic screen is 2 ~ 15cm.
6. a kind of method carrying out fish scrap bone and flesh separation using microbial fermentation according to claim 1, special
Sign exists
In：The step（6）The sieve pore of small aperture metallic screen is 0.01 ~ 2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610129692.9A CN105724550B (en) | 2016-03-09 | 2016-03-09 | A method of carrying out fish scrap bone and flesh separation using microbial fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610129692.9A CN105724550B (en) | 2016-03-09 | 2016-03-09 | A method of carrying out fish scrap bone and flesh separation using microbial fermentation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105724550A CN105724550A (en) | 2016-07-06 |
| CN105724550B true CN105724550B (en) | 2018-08-03 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610129692.9A Expired - Fee Related CN105724550B (en) | 2016-03-09 | 2016-03-09 | A method of carrying out fish scrap bone and flesh separation using microbial fermentation |
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| Country | Link |
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| CN (1) | CN105724550B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467674A (en) * | 2017-06-22 | 2017-12-15 | 乐清瑞耀食品技术有限公司 | Compound based on duckbilled fish active peptides |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3804964A (en) * | 1970-06-04 | 1974-04-16 | Astra Nutrition Ab | Method and apparatus for treating fish |
| CN101248821A (en) * | 2007-11-19 | 2008-08-27 | 广东海洋大学 | Process engineering for extracting fishes processing offal fish oil by enzymolysis process |
| CN101705219A (en) * | 2009-11-12 | 2010-05-12 | 集美大学 | Production method of protease hydrolyzing leftovers of freshwater fish and other fish efficiently |
| CN102150875A (en) * | 2011-01-21 | 2011-08-17 | 河南农业大学 | Enzymolysis method for bone dregs |
| CN102550804A (en) * | 2012-02-04 | 2012-07-11 | 广东海洋大学 | Method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals |
| CN104844292A (en) * | 2015-04-29 | 2015-08-19 | 漳州三本肥料工业有限公司 | Method for preparing fertilizer by using shrimp wastes and product thereof |
| KR101563109B1 (en) * | 2015-04-17 | 2015-10-23 | 황미경 | A mudfish bone and fish meat separation equipment make use of centrifugal force |
| CN105211616A (en) * | 2015-10-27 | 2016-01-06 | 广东海洋大学 | A kind of method of comprehensive utilization to shrimping beam trawl |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102792997B (en) * | 2012-08-10 | 2015-07-22 | 中国水产科学研究院东海水产研究所 | Method for separating shell and meat of small crustacean |
-
2016
- 2016-03-09 CN CN201610129692.9A patent/CN105724550B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3804964A (en) * | 1970-06-04 | 1974-04-16 | Astra Nutrition Ab | Method and apparatus for treating fish |
| CN101248821A (en) * | 2007-11-19 | 2008-08-27 | 广东海洋大学 | Process engineering for extracting fishes processing offal fish oil by enzymolysis process |
| CN101705219A (en) * | 2009-11-12 | 2010-05-12 | 集美大学 | Production method of protease hydrolyzing leftovers of freshwater fish and other fish efficiently |
| CN102150875A (en) * | 2011-01-21 | 2011-08-17 | 河南农业大学 | Enzymolysis method for bone dregs |
| CN102550804A (en) * | 2012-02-04 | 2012-07-11 | 广东海洋大学 | Method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals |
| KR101563109B1 (en) * | 2015-04-17 | 2015-10-23 | 황미경 | A mudfish bone and fish meat separation equipment make use of centrifugal force |
| CN104844292A (en) * | 2015-04-29 | 2015-08-19 | 漳州三本肥料工业有限公司 | Method for preparing fertilizer by using shrimp wastes and product thereof |
| CN105211616A (en) * | 2015-10-27 | 2016-01-06 | 广东海洋大学 | A kind of method of comprehensive utilization to shrimping beam trawl |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467674A (en) * | 2017-06-22 | 2017-12-15 | 乐清瑞耀食品技术有限公司 | Compound based on duckbilled fish active peptides |
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| Publication number | Publication date |
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| CN105724550A (en) | 2016-07-06 |
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