CN108065031A - A kind of microorganism conversion pineapple bran is the preparation method of biological feedstuff - Google Patents
A kind of microorganism conversion pineapple bran is the preparation method of biological feedstuff Download PDFInfo
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- CN108065031A CN108065031A CN201711416360.XA CN201711416360A CN108065031A CN 108065031 A CN108065031 A CN 108065031A CN 201711416360 A CN201711416360 A CN 201711416360A CN 108065031 A CN108065031 A CN 108065031A
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- pineapple
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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Abstract
It is disclosed by the invention to belong to microbiological feed technical field, it is specially the preparation method that a kind of microorganism conversion pineapple bran is biological feedstuff, which includes the following steps for the preparation method of biological feedstuff：S1：Pineapple Slag treatment：Choose commercially available pineapple processing waste, dry, crush it is spare；S2：Bacterial strain activates；S3：Saccharomycete culture；S4：Pineapple fermenting microbe culture：Pineapple bran in step S1 is added in solid fermentation culture medium, when fermentation 40 52 is small under 30 34 degrees Celsius；S5：Mixing：By malt medium, seed culture medium and the solid fermentation culture medium in step S2 according to 1:1:2 ratio stirs evenly, when culture 65 80 is small in the environment of sealing, using pineapple bran as raw material, by the fermentation to pineapple bran, the biological feedstuff of high protein is produced, energetically progradation is played to realization pineapple bran recycling industrialization, reduce the pollution of environment, energy conservation and environmental protection.
Description
Technical field
The present invention relates to microbiological feed technical fields, are specially the system that a kind of microorganism conversion pineapple bran is biological feedstuff
Preparation Method.
Background technology
Pineapple is one of tropical fruit (tree).Fujian and Taiwan be referred to as prosperous pears or it is prosperous come, Singapore and Malaysia one with being known as yellow pears,
Continent and Hong Kong are referred to as pineapple.There are one of a kind more than 70, four big, south of the Five Ridges fruit.Pineapple fruit is best in quality, full of nutrition, contains
There are substantial amounts of fructose, glucose, vitamin B, C, phosphorus, the substances such as citric acid and protease.Pineapple water content is high, is not easy to store,
In addition to being sold on a small quantity directly in the form of fresh fruit, most of portion pineapple is processed into the products such as pineapple tin, preserved fruit and sells to both at home and abroad.
In pineapple process, substantial amounts of pineapple bran is just constantly be generated, and accounts for the ratio of fresh fruit 60%.Pineapple bran cannot disappear in time
It removes, concentration is piled up near highway both sides or processing factory, seriously pollutes environment；Pineapple bran is unprocessed to be stacked at soil, carbohydrate hair
Ferment, which generates excessive organic acid, makes soil acidification, influences crop growth；Pineapple bran is abandoned in river, also to limited water resource
It pollutes.Biological feedstuff is generally referred to using feed and feed addictive as object, with genetic engineering, protein engineering, fermentation
The new and high technologies such as engineering are means, utilize the novel fodder resource and feed addictive of microbial project fermentation exploitation.Therefore adopt
The biological feedstuff that pineapple fermenting and producing is gone out to high protein with microorganism has a vast market prospect.It is for this purpose, it is proposed that a kind of
Microorganism conversion pineapple bran is the preparation method of biological feedstuff.
The content of the invention
It is an object of the invention to provide the preparation method that a kind of microorganism conversion pineapple bran is biological feedstuff, in solution
State the problem of being proposed in background technology.
To achieve the above object, the present invention provides following technical solution：A kind of microorganism conversion pineapple bran is biological feedstuff
Preparation method, which includes the following steps for the preparation method of biological feedstuff：
S1：Pineapple Slag treatment：Choose commercially available pineapple processing waste, dry, crush it is spare；
S2：Bacterial strain activates：Strain is transferred in malt medium, when culture 70-75 is small under 26-30 degrees Celsius；
S3：Saccharomycete culture：Saccharomycete is seeded to seed culture medium, it is small in 26-30 degrees Celsius of lower stir culture 36-40
When；
S4：Pineapple fermenting microbe culture：Pineapple bran in step S1 is added in solid fermentation culture medium, is taken the photograph in 30-34
When the 40-52 that ferments under family name's degree is small；
S5：Mixing：By malt medium, seed culture medium and the solid fermentation culture medium in step S2 according to 1:1:2
Ratio stirs evenly, when culture 65-80 is small in the environment of sealing.
Preferably, in the step S1, pineapple processing waste is needed during drying to pineapple processing waste
Sterilization treatment is carried out, during drying, the water content control of pineapple processing waste is in 10%-35%.
Preferably, in the step S1, the pineapple bran after crushing needed 10-20 mesh sieves.
Preferably, the strain in the step S2 is trichoderma viride and saccharomyces cerevisiae.
Preferably, stir speed (S.S.) is 180-200 revs/min in the step S3.
Preferably, the solid fermentation culture medium in the step S4 includes ammonium sulfate, potassium dihydrogen phosphate, peptone, lactose
And glucose.
Preferably, the material-water ratio of the solid fermentation culture medium in the step S4 is 2:1.
Preferably, the stir speed (S.S.) in the step S5 is 140-160 revs/min, and whipping temp is 30-40 degrees Celsius.
Compared with prior art, the beneficial effects of the invention are as follows：The invention propose a kind of microorganism conversion pineapple bran be
The preparation method of biological feedstuff, using pineapple bran as raw material, by the fermentation to pineapple bran, the biology for producing high protein is raised
Material to realizing that pineapple bran recycling industrialization plays energetically progradation, reduces the pollution of environment, realizes money
The reasonable utilization in source, energy conservation and environmental protection.
Description of the drawings
Fig. 1 is preparation method flow chart of the present invention.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work
Embodiment belongs to the scope of protection of the invention.
Referring to Fig. 1, the present invention provides the preparation method that a kind of microorganism conversion pineapple bran is biological feedstuff：
Embodiment 1
The microorganism conversion pineapple bran includes the following steps for the preparation method of biological feedstuff：
S1：Pineapple Slag treatment：Commercially available pineapple processing waste is chosen, dries, crush spare, pineapple processing waste is drying
It needs to carry out sterilization treatment to pineapple processing waste in dry process, during drying, pineapple processing waste contains
Pineapple bran of the water rate control after 10%-35%, crushing needed 20 mesh sieves；
S2：Bacterial strain activates：Strain is transferred in malt medium, when culture 75 is small under 30 degrees Celsius, strain is green
Color Trichoderma and saccharomyces cerevisiae；
S3：Saccharomycete culture：Saccharomycete is seeded to seed culture medium, when 30 degrees Celsius of lower stir cultures 40 are small, is stirred
Rate is mixed as 200 revs/min；
S4：Pineapple fermenting microbe culture：Pineapple bran in step S1 is added in solid fermentation culture medium, is taken the photograph in 30-34
When fermentation 52 is small under family name's degree, solid fermentation culture medium includes ammonium sulfate, potassium dihydrogen phosphate, peptone, lactose and glucose, solid
The material-water ratio of fermentation medium is 2:1；
S5：Mixing：By malt medium, seed culture medium and the solid fermentation culture medium in step S2 according to 1:1:2
Ratio stirs evenly, and when culture 80 is small in the environment of sealing, stir speed (S.S.) is 160 revs/min, and whipping temp is Celsius for 40
Degree.
Embodiment 2
The microorganism conversion pineapple bran includes the following steps for the preparation method of biological feedstuff：
S1：Pineapple Slag treatment：Commercially available pineapple processing waste is chosen, dries, crush spare, pineapple processing waste is drying
It needs to carry out sterilization treatment to pineapple processing waste in dry process, during drying, pineapple processing waste contains
Pineapple bran of the water rate control after 10%-35%, crushing needed 10 mesh sieves；
S2：Bacterial strain activates：Strain is transferred in malt medium, when culture 70 is small under 26 degrees Celsius, strain is green
Color Trichoderma and saccharomyces cerevisiae；
S3：Saccharomycete culture：Saccharomycete is seeded to seed culture medium, when 26 degrees Celsius of lower stir cultures 36 are small, is stirred
Rate is mixed as 180 revs/min；
S4：Pineapple fermenting microbe culture：Pineapple bran in step S1 is added in solid fermentation culture medium, at 30 degrees Celsius
It is lower fermentation 40 it is small when, solid fermentation culture medium include ammonium sulfate, potassium dihydrogen phosphate, peptone, lactose and glucose, solid fermentation
The material-water ratio of culture medium is 2:1；
S5：Mixing：By malt medium, seed culture medium and the solid fermentation culture medium in step S2 according to 1:1:2
Ratio stirs evenly, and when culture 65 is small in the environment of sealing, stir speed (S.S.) is 140 revs/min, and whipping temp is Celsius for 30
Degree.
Embodiment 3
The microorganism conversion pineapple bran includes the following steps for the preparation method of biological feedstuff：
S1：Pineapple Slag treatment：Commercially available pineapple processing waste is chosen, dries, crush spare, pineapple processing waste is drying
It needs to carry out sterilization treatment to pineapple processing waste in dry process, during drying, pineapple processing waste contains
Pineapple bran of the water rate control after 10%-35%, crushing needed 15 mesh sieves；
S2：Bacterial strain activates：Strain is transferred in malt medium, when culture 73 is small under 28 degrees Celsius, strain is green
Color Trichoderma and saccharomyces cerevisiae；
S3：Saccharomycete culture：Saccharomycete is seeded to seed culture medium, when 28 degrees Celsius of lower stir cultures 38 are small, is stirred
Rate is mixed as 190 revs/min；
S4：Pineapple fermenting microbe culture：Pineapple bran in step S1 is added in solid fermentation culture medium, at 32 degrees Celsius
It is lower fermentation 46 it is small when, solid fermentation culture medium include ammonium sulfate, potassium dihydrogen phosphate, peptone, lactose and glucose, solid fermentation
The material-water ratio of culture medium is 2:1；
S5：Mixing：By malt medium, seed culture medium and the solid fermentation culture medium in step S2 according to 1:1:2
Ratio stirs evenly, and when culture 72 is small in the environment of sealing, stir speed (S.S.) is 150 revs/min, and whipping temp is Celsius for 35
Degree.
In summary embodiment, the crude protein content of the biological feedstuff in embodiment 1 is 20.5%, in embodiment 2
The crude protein content of biological feedstuff is 22.4%, and the crude protein content of the biological feedstuff in embodiment 3 is 26.5%.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of microorganism conversion pineapple bran is the preparation method of biological feedstuff, it is characterised in that：The microorganism conversion pineapple bran
Include the following steps for the preparation method of biological feedstuff：
S1：Pineapple Slag treatment：Choose commercially available pineapple processing waste, dry, crush it is spare；
S2：Bacterial strain activates：Strain is transferred in malt medium, when culture 70-75 is small under 26-30 degrees Celsius；
S3：Saccharomycete culture：Saccharomycete is seeded to seed culture medium, when 26-30 degrees Celsius of lower stir culture 36-40 is small；
S4：Pineapple fermenting microbe culture：Pineapple bran in step S1 is added in solid fermentation culture medium, at 30-34 degrees Celsius
It is lower fermentation 40-52 it is small when；
S5：Mixing：By malt medium, seed culture medium and the solid fermentation culture medium in step S2 according to 1:1:2 ratio
It stirs evenly, when culture 65-80 is small in the environment of sealing.
2. a kind of microorganism conversion pineapple bran according to claim 1 is the preparation method of biological feedstuff, it is characterised in that：
In the step S1, pineapple processing waste needs to carry out sterilization treatment to pineapple processing waste during drying,
During drying, the water content control of pineapple processing waste is in 10%-35%.
3. a kind of microorganism conversion pineapple bran according to claim 1 is the preparation method of biological feedstuff, it is characterised in that：
In the step S1, the pineapple bran after crushing needed 10-20 mesh sieves.
4. a kind of microorganism conversion pineapple bran according to claim 1 is the preparation method of biological feedstuff, it is characterised in that：
Strain in the step S2 is trichoderma viride and saccharomyces cerevisiae.
5. a kind of microorganism conversion pineapple bran according to claim 1 is the preparation method of biological feedstuff, it is characterised in that：
Stir speed (S.S.) is 180-200 revs/min in the step S3.
6. a kind of microorganism conversion pineapple bran according to claim 1 is the preparation method of biological feedstuff, it is characterised in that：
Solid fermentation culture medium in the step S4 includes ammonium sulfate, potassium dihydrogen phosphate, peptone, lactose and glucose.
7. a kind of microorganism conversion pineapple bran according to claim 1 is the preparation method of biological feedstuff, it is characterised in that：
The material-water ratio of solid fermentation culture medium in the step S4 is 2:1.
8. a kind of microorganism conversion pineapple bran according to claim 1 is the preparation method of biological feedstuff, it is characterised in that：
Stir speed (S.S.) in the step S5 is 140-160 revs/min, and whipping temp is 30-40 degrees Celsius.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112438160A (en) * | 2019-09-02 | 2021-03-05 | 北京市房山区种植业技术推广站 | Ramaria strain and application thereof, and mother culture medium for artificially culturing Ramaria and application thereof |
| CN113397050A (en) * | 2021-07-23 | 2021-09-17 | 金银卡(广州)生物科技股份有限公司 | Preparation method of ultralow protein compound feed for fattening sheep |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802987A (en) * | 2006-01-17 | 2006-07-19 | 广东省农业科学院农业生物技术研究所 | Preparation method for transforming pineapple bran to biological feed stuff using microbe |
| CN101595940A (en) * | 2008-06-05 | 2009-12-09 | 广东海洋大学 | Method for producing feed protein by pineapple peel fermentation |
| CN102326672A (en) * | 2011-09-09 | 2012-01-25 | 薛晓宁 | Fermented feed protein prepared from pineapple peel and processing method thereof |
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2017
- 2017-12-25 CN CN201711416360.XA patent/CN108065031A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1802987A (en) * | 2006-01-17 | 2006-07-19 | 广东省农业科学院农业生物技术研究所 | Preparation method for transforming pineapple bran to biological feed stuff using microbe |
| CN101595940A (en) * | 2008-06-05 | 2009-12-09 | 广东海洋大学 | Method for producing feed protein by pineapple peel fermentation |
| CN102326672A (en) * | 2011-09-09 | 2012-01-25 | 薛晓宁 | Fermented feed protein prepared from pineapple peel and processing method thereof |
Non-Patent Citations (1)
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| 王向东等主编: "《食品生物技术》", 30 September 2007, 东南大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112438160A (en) * | 2019-09-02 | 2021-03-05 | 北京市房山区种植业技术推广站 | Ramaria strain and application thereof, and mother culture medium for artificially culturing Ramaria and application thereof |
| CN113397050A (en) * | 2021-07-23 | 2021-09-17 | 金银卡(广州)生物科技股份有限公司 | Preparation method of ultralow protein compound feed for fattening sheep |
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Application publication date: 20180525 |