CN1802987A - Preparation method for transforming pineapple bran to biological feed stuff using microbe - Google Patents
Preparation method for transforming pineapple bran to biological feed stuff using microbe Download PDFInfo
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- CN1802987A CN1802987A CNA2006100330242A CN200610033024A CN1802987A CN 1802987 A CN1802987 A CN 1802987A CN A2006100330242 A CNA2006100330242 A CN A2006100330242A CN 200610033024 A CN200610033024 A CN 200610033024A CN 1802987 A CN1802987 A CN 1802987A
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- pineapple bran
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Abstract
The invention discloses a process for preparing biological feeding-stuff by inverting pineapple bran with microorganism, inoculating a circular bacterial into seed culture medium, getting seed liquor by means of shake culture; inoculating Pseudomonas bacterial and bacillus subtilis into seed liquid in fluid enlarging LB medium with 1-5% inoculation consumption and inoculating lactic acid bacteria in PYG culture medium with 1-5% inoculation consumption, shake culturing, mixing every bacterial fermentation cultural liquid according to ratio of 1:1 as pineapple bran fermentation bacterial liquor; then inoculating which into pineapple bran, mixing evenly, compacting pineapple bran and loading into air-tight container for fermentation. The invention can eliminate environmental pollution caused by pineapple bran and at the same time produces green biological feeding-stuff of high nutritional value with the said waste.
Description
Technical field
The present invention relates to a kind of is raw material with the pineapple bran, utilizes microbial technique to transform the preparation method who produces biological feedstuff.
Background technology
Pineapple (Ananas comosus) is one of tropical large fruit, the nutrition succulence, and sweet is tempting, very popular; Pineapple is extensively planted in Guangdong Province, is that about 250,000 mu pineapple production base has been set up in main area in the Lezhou Peninsula, in Fujian, South China such as Hainan, Guangxi and south east asia extensively plant pineapple.Pineapple water content height be difficult for to store, except that a small amount of directly sell with the form of fresh fruit, most of portion pineapple is processed into goods such as pineapple tin, preserved fruit and sells to both at home and abroad.
With the continuous expansion of the cultivated area of pineapple, the pineapple processing enterprise of various big and small scales builds up one after another.In the pineapple process, a large amount of pineapple brans just constantly produces, and accounts for the ratio of fresh fruit 60%.Pineapple bran can not in time be eliminated, and concentrates and piles up near highway both sides or the processing factory serious environment pollution; The unprocessed soil that is stacked at of pineapple bran, carbohydrate fermentation produces too much organic acid and makes soil acidification, influences crop growth; Pineapple bran abandons in the river, also limited water resource is polluted.A large amount of pineapple bran generations is big, concentrated, continuous, causes local resident's protest, causes enough attention of local relevant department; Pineapple processing enterprise has to spend suitable fund and facility is handled pineapple bran.Therefore, the processing of pineapple bran has had a strong impact on pineapple processing enterprise and has further developed
Though the pineapple bran water content is higher, main component and pineapple pulp difference are little, and wherein protein content also is higher than pulp, and it is about 80% to contain NFE in the dry, fiber 8.5%, thick protein 4%, sugar amount about 30%.Pineapple bran has higher nutritional utilization and is worth.Present technique provides practicable reference scheme at the key issue of eliminating the pineapple bran pollution, simultaneously this discarded object is had the green bio feed that better nutritivity is worth as a kind of production; Can effectively implement to reach " eliminate bad pollution, make full use of resource, promote healthy aquaculture ", the purpose of achieving many things at one stroke.
Summary of the invention
The object of the present invention is to provide a kind of microbial fermentation pineapple bran to produce the preparation method of biological feedstuff,, reduce environmental pollution to improve the added value of pineapple bran.
Purpose of the present invention is achieved by the following technical programs:
Microbial conversion pineapple bran provided by the invention is the preparation method of biological feedstuff, the steps include:
A is picking one ring thalline from the original bacterial classification, is inoculated in the seed culture medium, and under 36-37 ℃ of temperature, 150-170rpm, shaking table is cultivated after 16-20 hour, up to OD
600=0.7-0.9 stops to cultivate, as seed liquor;
B. Pseudomonas stutzeri belong to bacterium and hay bacillus belong to bacterium in liquid enlarging LB culture medium, the genus lactubacillus bacterium in the PYG culture medium, each is with 1-5% inoculum concentration inoculation seed liquor, under 36-37 ℃ of temperature, 150-170rpm, shaking table was cultivated 20-28 hour.Various strain fermentation nutrient solutions are mixed in 1: 1 ratio, as pineapple bran fermented bacterium liquid;
C. the inoculum concentration of pineapple bran fermented bacterium liquid among the b with 0.3-5% is inoculated in the pineapple bran of water content 30%-50%, mix, with the pineapple bran compacting, make its inner no air, the closed container of packing into, stopped in 48-72 hour cultivating in 20-38 ℃ of temperature bottom fermentation, be positioned over the shady and cool dry place of room temperature.
Bacterial classification seed culture medium described in the step a of the present invention contains the glucose of 0.5-1.0%, the lactose of 0.3-0.5%, the sodium chloride of 0.3-0.5%, peptone and the 0.5-1.0% yeast extract of 0.5-1.0% by weight percentage, contain the sodium chloride of 0.3-0.5%, peptone and the 0.5-1.0% yeast extract of 0.3-0.5% at the LB culture medium described in the step b, the PYG culture medium contains the glucose of 0.5-1.0%, the lactose of 0.3-0.5%, the sodium acetate of 0.3-0.5%, peptone and the 0.5-1.0% yeast extract of 0.5-1.0%.
Bacterial classification described in the step a of the present invention is that Pseudomonas stutzeri belongs to bacterium (Pseudomonas srutzeri) 2 strains, hay bacillus belongs to bacterium (Bacillus subtilis) 3 strains and genus lactubacillus bacterium (Lactobacillus) 1 strain.
The present invention can eliminate the pineapple bran problem of environment pollution caused, simultaneously this discarded object is had the green bio feed that better nutritivity is worth as a kind of production, can effectively implement to reach the effect of " eliminate bad pollution, make full use of resource, promote healthy aquaculture ".Through the pineapple bran pH value 2.0-3.0 that the present invention handles, the golden yellow color of finished product after smell fragrant, pineapple bran ferment in closed container, at room temperature can be deposited 6-9 month, to needing the time spent, took out from closed container.
The pineapple bran of the present invention's preparation can be used as the biological feedstuff of animals such as milk cow, Tilapia mossambica, laying hen, can improve the feed intake of animal (milk cow, laying hen), the output of milk of milk cow more significantly.Test effect is as follows:
(1) selects 180 the 3 healthy laying hens of age in days, be divided into 2 groups (control group, fermentation pineapple bran group) at random, every group of 3 repetitions, 30 chickens of every repetition.Control group fed basal diet, processed group then add 4% pineapple bran on the basis of basal diet, experimental period was 4 week.Off-test, daily gain, daily ingestion amount and the feed consumption weightening finish of measuring chicken are than three indexs.The result shows: compare with control group, behind the interpolation pineapple bran, nascent chicken daily gain, feed intake have improved 14.16%, 0.7% respectively, and diarrhea rate has reduced by 38% (table 1).
Table 1 pineapple bran is to the effect of nascent chicken
| Experimental animal | Group | Daily gain (g/d) | Daily ingestion amount (g/d) | Feed consumption weightening finish ratio | Diarrhea rate (%) |
| Nascent chicken | The control fermentation pineapple bran | 17.2±2.3 19.6±1.5 | 33.7±2.2 34.0±3.1 | 1.96±0.22 1.73±0.17 | 33±4 19±1 |
(2) in the Tilapia mossambica batch, mix and feed 5% fermentation pineapple bran, the contrast pond pineapple bran that need not ferment, experimental period is 120 days.It is 55～58 tails/500g that test is just put the fish specification in a suitable place to breed, and breeding density is 2.55 ten thousand tails/hm
2Compare with the contrast pond, add the fermentation pineapple bran and make the survival rate of Sarotherodon sp improve 3.1%, every hm
2Single rate has improved 16% (table 2).
The effect of table 2 fermentation pineapple bran on Sarotherodon sp
| Group | Test area | Results specification (g/ tail) | Per unit area yield (t/bm 2) | Survival rate (%) |
| Contrast pond test tank | 0.813hm 2 0.920hm 2 | 653±55 755±33 | 14.83 17.30 | 95 98 |
(3) with the pineapple bran after the fermentation process as ensilage, feeding cow is after 10 days, the test group output of milk of searching for food all can recover normal; The test middle and later periods, test group and control group compare, and feed intake improves 17.76%, and the output of milk has improved 27.79%, and protein ratio has improved 3.23%, and milk dry matter content has improved 11.98%, the butterfat percnetage kept stable.
The specific embodiment
The embodiment that a kind of microbial fermentation pineapple bran provided by the invention is produced biological feedstuff is as follows:
Present embodiment 1 used bacterial classification is that Pseudomonas stutzeri belongs to bacterium (Pseudomonas stutzeri) 2 strains, hay bacillus belongs to bacterium (Bacillus subtilis) 3 strains and genus lactubacillus bacterium (Lactobacillus) 1 strain.The steps include:
A. the bacterial classification seed culture medium of being made up of 5 gram glucose, 3 gram lactose, 3 gram sodium chloride, 5 gram peptones, 5 gram yeast extracts and 1 premium on currency (pH6.5) is poured in the aseptic triangular flask, makes the seed fluid nutrient medium; By the LB fluid nutrient medium that 3 gram sodium chloride, 3 gram peptones, 5 gram yeast extracts and 1 premium on currency (pH6.5) are formed, pour in the aseptic triangular flask, make the LB fluid nutrient medium; By the PYG fluid nutrient medium that lactose, 3 gram sodium acetates, 5 gram peptones, 5 gram yeast extracts and 1 premium on currency (pH6.5) of 5 gram glucose, 3 grams are formed, pour in the aseptic triangular flask, make the PYG fluid nutrient medium.
B. with aseptic inoculation ring picking one ring thalline from the original bacterial classification, be inoculated in the seed fluid nutrient medium, under 36 ℃ of temperature, the 150rpm shaking table was cultivated 16 hours;
C. treat seed liquid medium OD
600=0.7 o'clock, stop to cultivate, this seed liquid medium is promptly as seed liquor;
D. in liquid enlarging LB culture medium in (Pseudomonas stutzeri belongs to bacterium, hay bacillus belongs to bacterium) and the PYG culture medium (genus lactubacillus bacterium) with 1% inoculum concentration inoculation seed liquor, under 36 ℃ of temperature, 150rpm, shaking table cultivation 20 hours.6 kinds of strain fermentation nutrient solutions are mixed in 1: 1 ratio, as pineapple bran fermented bacterium liquid.
E. pineapple bran fermented bacterium liquid is inoculated in the pineapple bran of water content 30% with 0.1% inoculum concentration, mixes, the pineapple bran compacting, make its inner no air, the closed container of packing into stopped to cultivate in 20 ℃ of temperature bottom fermentations in 48 hours, was positioned over the shady and cool dry place of room temperature;
F. after the pineapple bran among the step e being fermented in closed container, can not arbitrarily take out from closed container, must wait needs the time spent, can take out (promptly the pineapple bran after the fermentation can not contact oxygen, otherwise can go bad);
G. the pineapple bran after fermenting among the step f promptly can be used as the feed of milk cow, Tilapia mossambica, laying hen etc.
Present embodiment 2 used bacterial classifications are that Pseudomonas stutzeri belongs to bacterium (Pseudomonas stutzeri) 2 strains, hay bacillus belongs to bacterium (Bacillus subtilis) 3 strains and genus lactubacillus bacterium (Lactobacillus) 1 strain.The steps include:
A. the bacterial classification seed culture medium of being made up of 8 gram glucose, 4 gram lactose, 4 gram sodium chloride, 8 gram peptones, 8 gram yeast extracts and 1 premium on currency (pH6.5) is poured in the aseptic triangular flask, makes the seed fluid nutrient medium; By the LB fluid nutrient medium that 4 gram sodium chloride, 4 gram peptones, 8 gram yeast extracts and 1 premium on currency (pH6.5) are formed, pour in the aseptic triangular flask, make the LB fluid nutrient medium; By the PYG fluid nutrient medium that lactose, 4 gram sodium acetates, 8 gram peptones, 8 gram yeast extracts and 1 premium on currency (pH6.5) of 8 gram glucose, 4 grams are formed, pour in the aseptic triangular flask, make the PYG fluid nutrient medium.
B. with aseptic inoculation ring picking one ring thalline from the original bacterial classification, be inoculated in the seed fluid nutrient medium, under 36.5 ℃ of temperature, the 160rpm shaking table was cultivated 18 hours;
C. treat seed liquid medium OD
600=0.8 o'clock, stop to cultivate, as seed liquor;
D. in liquid enlarging LB culture medium in (Pseudomonas stutzeri belongs to bacterium, hay bacillus belongs to bacterium) and the PYG culture medium (genus lactubacillus bacterium) with 3% inoculum concentration inoculation seed liquor, under 36.5 ℃ of temperature, 160rpm, shaking table was cultivated 24 hours.6 kinds of strain fermentation nutrient solutions are mixed in 1: 1 ratio, as pineapple bran fermented bacterium liquid.
E. pineapple bran fermented bacterium liquid is inoculated in the pineapple bran of water content 40% with 3% inoculum concentration, mixes, the pineapple bran compacting, make its inner no air, the closed container of packing into stopped to cultivate in 29 ℃ of temperature bottom fermentations in 56 hours, was positioned over the shady and cool dry place of room temperature.
Present embodiment 3 used bacterial classifications are that Pseudomonas stutzeri belongs to bacterium (Pseudomonas stutzeri) 2 strains, hay bacillus belongs to bacterium (Bacillus subtilis) 3 strains and genus lactubacillus bacterium (Lactobacillus) 1 strain.The steps include:
A. the bacterial classification seed culture medium of being made up of 10 gram glucose, 5 gram lactose, 5 gram sodium chloride, 10 gram peptones, 10 gram yeast extracts and 1 premium on currency (pH6.5) is poured in the aseptic triangular flask, makes the seed fluid nutrient medium; By the LB fluid nutrient medium that 5 gram sodium chloride, 5 gram peptones, 10 gram yeast extracts and 1 premium on currency (pH6.5) are formed, pour in the aseptic triangular flask, make the LB fluid nutrient medium; By the PYG fluid nutrient medium that lactose, 5 gram sodium acetates, 10 gram peptones, 10 gram yeast extracts and 1 premium on currency (pH6.5) of 10 gram glucose, 5 grams are formed, pour in the aseptic triangular flask, make the PYG fluid nutrient medium.
B. with aseptic inoculation ring picking one ring thalline from the original bacterial classification, be inoculated in the seed fluid nutrient medium, under 37 ℃ of temperature, the 170rpm shaking table was cultivated 20 hours;
C. treat seed liquid medium OD
600=0.9 o'clock, stop to cultivate, promptly as seed liquor;
D. in liquid enlarging LB culture medium in (Pseudomonas stutzeri belongs to bacterium, hay bacillus belongs to bacterium) and the PYG culture medium (genus lactubacillus bacterium) with 5% inoculum concentration inoculation seed liquor, under 37 ℃ of temperature, 170rpm, shaking table cultivation 28 hours.6 kinds of strain fermentation nutrient solutions are mixed in 1: 1 ratio, as pineapple bran fermented bacterium liquid.
E. pineapple bran fermented bacterium liquid is inoculated in the pineapple bran of water content 50% with 5% inoculum concentration, mixes, the pineapple bran compacting, make its inner no air, the closed container of packing into stopped to cultivate in 38 ℃ of temperature bottom fermentations in 72 hours, was positioned over the shady and cool dry place of room temperature.
Claims (4)
1, a kind of microbial conversion pineapple bran preparation method that is biological feedstuff is characterized in that step is:
A is picking one ring thalline from the original bacterial classification, is inoculated in the seed culture medium, and under 36-37 ℃ of temperature, 150-170rpm, shaking table is cultivated after 16-20 hour, up to OD
600=0.7-0.9 stops to cultivate, as seed liquor;
B. Pseudomonas stutzeri belong to bacterium and hay bacillus belong to bacterium in liquid enlarging LB culture medium, the genus lactubacillus bacterium in the PYG culture medium, each is with 1-5% inoculum concentration inoculation seed liquor, under 36-37 ℃ of temperature, 150-170rpm, shaking table was cultivated 20-28 hour; Various strain fermentation nutrient solutions are mixed in 1: 1 ratio, as pineapple bran fermented bacterium liquid;
C. the inoculum concentration of pineapple bran fermented bacterium liquid among the b with 0.3-5% is inoculated in the pineapple bran of water content 30%-50%, mix, with the pineapple bran compacting, make its inner no air, the closed container of packing into, stopped in 48-72 hour cultivating in 20-38 ℃ of temperature bottom fermentation, be positioned over the shady and cool dry place of room temperature.
2, the microbial conversion pineapple bran according to claim 1 preparation method that is biological feedstuff is characterized in that the bacterial classification seed culture medium described in the step a contains peptone and the 0.5-1.0% yeast extract of the sodium chloride of the lactose of the glucose of 0.5-1.0%, 0.3-0.5%, 0.3-0.5%, 0.5-1.0% by weight percentage.
3, the microbial conversion pineapple bran according to claim 1 preparation method that is biological feedstuff is characterized in that the LB culture medium described in the step b contains peptone and the 0.5-1.0% yeast extract of the sodium chloride of 0.3-0.5%, 0.3-0.5%; The PYG culture medium contains the glucose of 0.5-1.0%, the lactose of 0.3-0.5%, the sodium acetate of 0.3-0.5%, peptone and the 0.5-1.0% yeast extract of 0.5-1.0%.
4, the microbial conversion pineapple bran according to claim 1 preparation method that is biological feedstuff, the bacterial classification described in its step a is that Pseudomonas stutzeri belongs to bacterium (Pseudomonas stutzeri) 2 strains, hay bacillus belongs to bacterium (Bacillus subtilis) 3 strains and genus lactubacillus bacterium (Lactobacillus) 1 strain.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444252B (en) * | 2008-11-18 | 2011-06-08 | 广东省农业科学院农业生物技术研究所 | Method for preparing fermented virus-free cottonseed meal feed protein |
| CN102150746A (en) * | 2010-12-28 | 2011-08-17 | 广东海洋大学 | Packaged and fermented pineapple dreg feed and energy-saving processing method thereof |
| CN101617737B (en) * | 2009-07-31 | 2012-07-25 | 广东希普生物科技股份有限公司 | Method for using pineapple peel and slag to prepare high activity dried yeast and ferment feed |
| CN103027188A (en) * | 2013-01-16 | 2013-04-10 | 合浦果香园食品有限公司 | Deep processing method of pineapple skin and residues |
| CN103039707A (en) * | 2012-12-26 | 2013-04-17 | 青岛盛嘉信息科技有限公司 | Pineapple waste-containing feed additive |
| CN103416593A (en) * | 2013-08-30 | 2013-12-04 | 张松波 | Method for manufacturing feed additives through jack fruit pericarps and pomace |
| CN108065031A (en) * | 2017-12-25 | 2018-05-25 | 陈益德 | A kind of microorganism conversion pineapple bran is the preparation method of biological feedstuff |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3413169B2 (en) * | 2000-04-03 | 2003-06-03 | 株式会社近藤榮一商店 | Fattening method of beef or pork |
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2006
- 2006-01-17 CN CNB2006100330242A patent/CN100358434C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444252B (en) * | 2008-11-18 | 2011-06-08 | 广东省农业科学院农业生物技术研究所 | Method for preparing fermented virus-free cottonseed meal feed protein |
| CN101617737B (en) * | 2009-07-31 | 2012-07-25 | 广东希普生物科技股份有限公司 | Method for using pineapple peel and slag to prepare high activity dried yeast and ferment feed |
| CN102150746A (en) * | 2010-12-28 | 2011-08-17 | 广东海洋大学 | Packaged and fermented pineapple dreg feed and energy-saving processing method thereof |
| CN103039707A (en) * | 2012-12-26 | 2013-04-17 | 青岛盛嘉信息科技有限公司 | Pineapple waste-containing feed additive |
| CN103027188A (en) * | 2013-01-16 | 2013-04-10 | 合浦果香园食品有限公司 | Deep processing method of pineapple skin and residues |
| CN103416593A (en) * | 2013-08-30 | 2013-12-04 | 张松波 | Method for manufacturing feed additives through jack fruit pericarps and pomace |
| CN108065031A (en) * | 2017-12-25 | 2018-05-25 | 陈益德 | A kind of microorganism conversion pineapple bran is the preparation method of biological feedstuff |
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