CN106854627B - Compound microbial inoculum for promoting growth of hermetia illucens larvae and application - Google Patents
Compound microbial inoculum for promoting growth of hermetia illucens larvae and application Download PDFInfo
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Abstract
The invention discloses a compound microbial inoculum for promoting the growth of hermetia illucens larvae and application thereof. The invention provides a compound microbial inoculum for promoting the growth of hermetia illucens larvae, which comprises the following components: corkspora bacteria (C.) (Kocuria marina）FE01，Lysinibacillus boronitoleransFE04, Proteus mirabilis (A)Proteus mirabilis) FE08 andBacillus subtilisthe BSF-CL is used for promoting the growth of the soldier flies in the process of converting the soldier flies into the chicken manure, and is safe and environment-friendly. By adding the microbial inoculum into the chicken manure containing the stratiomyiid, the weight gain of the stratiomyiid is improved by 28.57 percent compared with that of the stratiomyiid of a stratiomyiid-chicken manure conversion system without adding the microbial inoculum, and the microbial inoculum can obviously improve the economic benefit in large-scale stratiomyiid conversion of the stratiomyiid into the chicken manure and has great economic value.
Description
Technical Field
The invention belongs to the fields of agricultural microbiology, ecological agriculture, environmental protection and the like, and particularly relates to a compound microbial inoculum for promoting the growth of hermetia illucens larvae and application thereof.
Background
1. Hermetia illucens (Levl.) Rapai
Hermetiaillucens L, known as Black soldier fly, is an insect of the species Hermetiaillucens of the genus Hermetia, the family Hermetiacidae, the order Diptera. Its larvae, up to 20mm long, originated in north america and have now become a widespread worldwide insect, distributed between 45 ° north latitude and 40 ° south latitude, recorded in america, europe, asia, oceania and many pacific islands. The hermetia illucens in China are mainly distributed in China and south China. The growth and development period of the hermetia illucens is divided into egg stage, larval stage, pupal stage and adult stage, the larval stage can be divided into 6 different instar stages, the 6 th larva is called prepupa, and the head of the larva in the period is chitified and does not eat any more. The stratiomyiid illucens can live through the winter by the larvae, the larvae enter a resting stage at low temperature, the stratiomyiid illucens can grow rapidly at constant temperature (about 28 ℃), the body length of 12d hatched larvae can reach 17mm, and the growth stage of the larvae can be completed 21 days after hatching.
The hermetia illucens larvae feed on rotten organic matters and animal wastes in nature, such as sour cherimoya, pumpkins, dead crabs, rotten fruits, animal carcasses and the like. Researches show that the soldier fly larvae can intensively eat animal excrement, so that pollution caused by excrement accumulation can be reduced, odor generated by excrement can be eliminated, breeding of houseflies can be controlled, and the number of escherichia coli in the excrement can be reduced. The hermetia illucens larva can be used for treating wastes, chicken manure, cow manure and chicken manure in coffee processing, and achieves good effect. The Hermetia illucens larvae contain a large amount of protein and fat, and can also be used for producing animal feed with high economic value.
2. Animal manure
With the adjustment of agricultural structures, the novel intensive cultivation is gradually replacing the traditional scattered household cultivation, the discharge amount of the livestock and poultry manure is larger and larger, according to statistics, the discharge amount of the livestock and poultry manure reaches 45 hundred million tons at present in China, and the pollution to the environment is very prominent, so that the harmless treatment of the livestock and poultry manure is closely concerned. The traditional treatment methods of the livestock and poultry manure mainly comprise three major methods, namely a physical method, a chemical method and a biological method. The physical method and the chemical method are time-consuming and energy-consuming in treatment, have poor treatment effect and can cause secondary pollution to the environment; the biological treatment method comprises several methods such as an anaerobic pond, an aerobic pond, composting and the like, and has the characteristics of low cost, strong activity of fermentation products and the like, but the common biological method has the problems of long conversion period, incapability of realizing high-value products and the like.
3. Insect intestinal microorganisms
Insect intestines harbor a large number of microorganisms, which have important influences and effects on their hosts. Early research on insect-microorganisms focused mainly on entomopathogenic microorganisms and their applications. With the progress of microbiology and micro ecology of insects, the research on the reciprocal symbiosis relationship between insects and microorganisms is more and more prominent, and the non-pathogenic relationship includes the reciprocal relationship, and some microorganisms are necessary for the growth and reproduction of hosts (Ben-yosef et al, 2008; Bright and Bulugheri, 2010; Drew et al, 1983). The more detailed researches are mainly the social insects of the termites and the agricultural pests of the locusts in desert and the like. Some insects make outstanding contributions in the utilization of lignocellulose energy, and high-activity cellulase, hemicellulase and the like are all derived from insects (Zhangli, 2011). The action of intestinal microorganisms on insects is mainly to participate in metabolism, provide nutrients, help hosts to resist the invasion of foreign pathogens, and stimulate host immune functions (good luck and huangyong ping, 2008). Jeon et al (2011) analyzed the microbial population in the intestinal tract of soldier fly larvae eating three different foods by using 16s rDNA sequencing, the microbial species were different according to foods but belong to four broad groups, and the strains producing amylase, protease and cellulase were isolated and cultured, and it is presumed that the degradation of organic matters by soldier flies may be related to microorganisms. Their studies, however, do not give direct evidence or data regarding the relationship of soldier flies to microorganisms in the degradation of waste. The stratiomyiid intestinal tract contains high amylase, lipase and protease activities, so that a foundation is provided for efficient degradation of waste, the stratiomyiid belongs to omnivorous animals, and the activity of various enzymes in the stratiomyiid intestinal tract is obviously higher than that of houseflies, which is probably the reason that the efficiency of the stratiomyiid in utilizing the waste is higher than that of other insects (Kim et al, 2011). However, this result was derived from the analysis of the enzyme activity throughout the gut and failed to separate the role that the microorganisms play in it.
At present, the soldier fly is used as a resource insect utilizing various organic wastes, and the grain and farming organization of the united nations puts the soldier fly as the most promising insect for human food and animal feed in the future in the first place of research and development. The research and development of the influence of the growth of the microorganisms and the soldier flies are still in the initial stage, and the method has important development value.
Disclosure of Invention
The invention aims to provide a compound microbial inoculum for promoting the growth of hermetia illucens larvae, and the compound microbial inoculum can promote the growth of hermetia illucens and transform livestock and poultry manure.
The invention also aims to provide an application of the compound microbial inoculum for promoting the growth of the soldier fly larvae, which comprises the steps of promoting the growth of the soldier fly larvae and promoting the conversion of chicken manure.
Still another object of the present invention is to provide Bacillus subtilis BSF-CL, which has been delivered to the chinese type culture collection for storage at 23/5/2014, under the classification name: bacillus subtilis BSF-CL, accession number: CCTCC NO: m2014222, address: wuhan university in Wuhan, China.
The last purpose of the invention is to provide the application of Bacillus subtilis BSF-CL, which comprises the steps of promoting the growth of hermetia illucens larvae, improving the survival rate of the hermetia illucens larvae, promoting excrement conversion and the like.
In order to achieve the purpose, the invention adopts the following technical measures:
the applicant separates 4 strains of bacteria from the surfaces and intestinal tracts of soldier flies, and the preservation information and physiological and biochemical characteristics of the four strains of bacteria are as follows:
and (3) Corkspora cubensis: and (3) classification and naming: kocuria marina (Kocuria marina) FE01, accession No.: CCTCC NO: m2015558; has been sent to the China center for type culture Collection on 9/18/2015, address: wuhan university in Wuhan, China. Physiological and biochemical characteristics: the colony on the LB culture medium is yellow, and the colony is neat in edge, convex, moist, free of wrinkles and medium in size. Gram-positive cocci, cultured at 28 ℃ and aerobes. Has protease (Up-1.60), cellulase (Up-2.00) and amylase (Up-2.50) hydrolysis activity.
B-lysine resistant bacillus: and (3) classification and naming: lysinibacillus boronitolerans FE04, accession number: CCTCC NO: m2015559; has been sent to the China center for type culture Collection on 9/18/2015, address: wuhan university in Wuhan, China. Physiological and biochemical characteristics: the colony on the LB culture medium is white, and the colony is neat in edge, convex, moist, free of wrinkles and medium in size. Gram-positive short rods, cultured at 28 ℃ and aerobic bacteria. Has protease (Up 1.45), cellulase (Up 1.70) and amylase (Up 2.30) hydrolysis activity.
Proteus mirabilis: proteus mirabilis (Proteus mirabilis) FE08, accession number: CCTCC NO: m2015560; has been sent to the China center for type culture Collection on 9/18/2015, address: wuhan university in Wuhan, China. Physiological and biochemical characteristics: the bacterial lawn has obvious polymorphism, spherical and filamentous shapes on LB culture medium, and is in diffusion growth on LB solid culture medium to form migratory growth phenomenon and wet bacterial lawn surface. Is gram-positive. Culturing at 28 deg.C, and aerobic culturing.
B, bacillus subtilis: and (3) classification and naming: bacillus subtilis BSF-CL with a deposit number: CCTCC NO: and M2014222. Has been sent to the China center for type culture Collection on 23/5/2014, address: wuhan university in Wuhan, China. Physiological and biochemical characteristics: the surface of the colony on the LB culture medium is rough, opaque and yellowish, and the skin becomes more and more formed when the colony grows in the LB liquid culture medium. Culturing at 28 deg.C, and aerobic culturing. Has protease (Up 2.18), cellulase (Up 1.19) and amylase (Up 5.38) hydrolysis activity.
A composite microbial inoculum for promoting the growth of hermetia illucens larvae comprises: cocurella (Kocuria marina) FE01, Lysinibacillus boronolans FE04, Proteus mirabilis (Proteus mirabilis) FE08 and Bacillus subtilis BSF-CL.
The compound microbial inoculum has the following preferred mixture ratio: kocuria (Kocuria marina) FE 01: lysinibacillus boronitolerans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis BSF-CL is 0.5-5: 0.5-5: 0.5-5. The effective concentration of each bacterium is the same.
The compound microbial inoculum has the following preferred mixture ratio: kocuria (Kocuria marina) FE 01: lysinibacillus boronitolerans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis BSF-CL is 1-4: 1-4: 1-4.
The compound microbial inoculum comprises the following microbial inocula in an optimal ratio: kocuria (Kocuria marina) FE 01: lysinibacillus boronitolerans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis BSF-CL 4: 1:1:1.
The application of the compound microbial inoculum for promoting the growth of the soldier fly larvae of the hermetia illucens comprises the step of adding the microbial inoculum into a mixture mixed with the soldier fly and excrement.
The application of the Bacillus subtilis BSF-CL comprises the step of adding the Bacillus subtilis BSF-CL into a mixture mixed with soldier fly and excrement.
Compared with the prior art, the invention has the following advantages:
1. the symbiotic bacteria derived from the soldier flies are used for promoting the growth of the soldier flies in the process of converting the soldier flies into the chicken manure, and are safe and environment-friendly.
2. The method is used for promoting the growth of the soldier flies in the process of converting the soldier flies into chicken manure by compounding symbiotic bacteria derived from the soldier flies for the first time.
3. The growth promotion effect of the compounded hermetia illucens-derived symbiotic bacteria on the hermetia illucens is the highest in the world at present (the weight gain of the hermetia illucens is improved by 28.57% compared with that of the hermetia illucens of a hermetia illucens chicken manure conversion system without bacteria), and in large-scale hermetia illucens conversion of chicken manure, the economic benefit can be obviously improved, and the great economic value is achieved.
Detailed Description
The experimental procedures in the following examples are conventional unless otherwise specified. The stratiomyiid used in the embodiment of the invention is stratiomyiid which is a stratiomyiid of solidus leuciscus Wuhan (Hermitiillucens strain Wuhan).
Example 1:
obtaining of the strain:
the applicant separates 4 strains of bacteria from the surfaces and intestinal tracts of soldier flies, and the preservation information and physiological and biochemical characteristics of the four strains of bacteria are as follows:
cocklebur (invention or FE 01): and (3) classification and naming: kocuria marina (Kocuria marina) FE01, accession No.: CCTCC NO: m2015558; has been sent to the China center for type culture Collection on 9/18/2015, address: wuhan university in Wuhan, China. Physiological and biochemical characteristics: the colony on the LB culture medium is yellow, and the colony is neat in edge, convex, moist, free of wrinkles and medium in size. Gram-positive cocci, cultured at 28 ℃ and aerobes. Has protease (Up-1.60), cellulase (Up-2.00) and amylase (Up-2.50) hydrolysis activity.
B-lysine resistant bacillus (or FE 04): and (3) classification and naming: lisibacillus boronolans FE04, accession number: CCTCC NO: m2015559; has been sent to the China center for type culture Collection on 9/18/2015, address: wuhan university in Wuhan, China. Physiological and biochemical characteristics: the colony on the LB culture medium is white, and the colony is neat in edge, convex, moist, free of wrinkles and medium in size. Gram-positive short rods, cultured at 28 ℃ and aerobic bacteria. Has protease (Up 1.45), cellulase (Up 1.70) and amylase (Up 2.30) hydrolysis activity.
Proteus mirabilis (or FE08) of the present invention: proteus mirabilis (Proteus mirabilis) FE08, accession number: CCTCC NO: m2015560; has been sent to the China center for type culture Collection on 9/18/2015, address: wuhan university in Wuhan, China. Physiological and biochemical characteristics: has obvious polymorphism on LB culture medium and has a spherical shapeAnd in a thread-like form inFixing device Body culture mediumThe upper part of the mushroom grows in a diffusion mode, the phenomenon of migratory growth is formed, and the surface of the lawn is wet. Is gram-positive. Culturing at 28 deg.C, and aerobic culturing.
Bacillus subtilis (or BSF-CL) of the invention: and (3) classification and naming: bacillus subtilis BSF-CL with a deposit number: CCTCC NO: m2014222. Has been sent to the China center for type culture Collection on 23/5/2014, address: wuhan university in Wuhan, China. Physiological and biochemical characteristics: on LB mediumBacterial colonyRough and opaque surface, yellowish colorLiquid culture Nutrient mediumWhen growing in the middle, wrinkle often forms. Culturing at 28 deg.C, and aerobic culturing. Has protease (Up 2.18), cellulase (Up 1.19) and amylase (Up 5.38) hydrolysis activity.
Identification of strains
FE01, FE04, FE08 and BSF-CL were preliminarily classified and identified by sequence homology analysis of 16SrDNA fragment, and the identification results are shown in Table 1.
TABLE 1 identification of the strains
Example 2:
fermentation method of single strain of FE01, FE04, FE08 and BSF-CL:
FE 01: LB liquid culture medium, the inoculum size is 1%, 180r/min, shaking flask fermentation 24h at 28 ℃.
FE 04: LB liquid culture medium, the inoculum size is 1%, 180r/min, shaking flask fermentation 24h at 28 ℃.
FE 08: LB liquid culture medium, the inoculum size is 1%, 180r/min, shaking flask fermentation 24h at 28 ℃.
BSF-CL: LB liquid culture medium, the inoculum size is 1%, 180r/min, shaking flask fermentation 24h at 28 ℃.
Example 2:
the application of FE01, FE04, FE08 and BSF-CL single strain fermentation liquor:
10ml of LB liquid medium was inoculated into a PA flask. Using sterile inoculating loop to pick out FE01, FE04, FE08 and BSF-CL single colonies respectively, and inoculating to PIn a bottle A, shaking the bottle for fermentation at 28 ℃ for 24 h. Adjusting the concentration of the fermentation broth to 1 × 10 with sterile water8And (2) mixing the diluted bacterial liquid with the chicken manure according to the proportion per ml, which is shown in the table below.
After the matrix is uniformly stirred, 500 healthy 6-day-old hermetia illucens larvae with similar sizes are simultaneously inoculated into different treatment groups, and each treatment is repeated for 3 times.
After 50% of stratiomyiid prepupa, stratiomyiid is separated from the matrix, and the total dry weight of the stratiomyiid and the dry weight of the residual matrix are counted after the stratiomyiid is dried at 50 ℃. The experimental results were counted using SPSS18.0 statistical analysis software.
The survival rate (%) of the soldier fly is equal to the number of transformed soldier flies/the number of soldier flies added before transformation is multiplied by 100 percent
The percent weight gain (%) of the insect (dry weight of hermetia illucens in the experimental group after transformation-dry weight of hermetia illucens in the control group after transformation)/dry weight of hermetia illucens in the control group after transformation
(ii) stratiomyiid conversion (%) - (dry weight of stratiomyiid after transformation-dry weight of stratiomyiid before transformation)/dry weight of chicken manure before transformation × 100%
The reduction ratio (%) of chicken manure (dry weight of chicken manure before transformation-dry weight of chicken manure after transformation)/dry weight of chicken manure before transformation x 100%
Growth promoting effects of single strain fermentation broths of FE01, FE04, FE08 and BSF-CL on soldier flies in chicken manure, see Table 2:
TABLE 2 Effect of addition of Individual strains of microorganisms on the survival rates and transformation cycles of Hermetia illucens
Note: using SPSS v19.0, the same letter after the average indicates no significance, the different letter indicates significance (P ═ 0.05), and the same applies below
1The time (d) required for 50% of the chicken manure to form prepupa was started from the transformation of the soldier fly larvae, and the experiment was repeated twice as follows.
As can be seen from table 2, in the two repetitions, the survival rates of the stratiomyiid larvae in different treatment groups are remarkably improved compared with the blank control group, the survival rate can be improved by 3.4% -3.47% by adding FE01, the survival rate can be improved by 4.13% -4.93% by adding FE04, the survival rate can be improved by 5.2% -5.33% by adding FE08, and the survival rate can be improved by 5.40-5.60% by adding BSF-CL, which indicates that the survival rate of the stratiomyiid larvae can be improved by the microorganisms. From the effect of microorganisms on the conversion period of the soldier fly into the chicken manure, the FE01 strain can shorten the conversion period by 2 days, and other strains have no effect on the conversion period of the chicken manure basically. However, from a general observation, and considering human error, the addition of microorganisms does not affect the transformation cycle of soldier fly larvae.
The effect of FE01, FE04, FE08 and BSF-CL single strain broths on stratiomyiid conversion and weight gain, see table 3:
TABLE 3 Effect of addition of Individual strains of microorganisms on Hermetia illucens conversion and weight gain
As can be seen from Table 3, the influence of the microorganisms in the two repeated experiments on the transformation rate and the weight gain rate of the soldier fly is the same, and compared with the blank control group, the influence of the addition of a single strain of microorganism on the transformation rate of the soldier fly is greatly different. The addition of FE01, FE08 and BSF-CL can obviously increase the weight of the soldier fly larvae. The weight gain rate of the larva of the experimental group added with FE08 can reach 18.6 percent, and the weight gain rate of the experimental group added with BSF-CL can reach 18.23 percent; the FE04 group showed two repeated experiments, compared with the control, the conversion and weight gain rate decreased slightly for one time, and increased slightly for the other time.
The effect of FE01, FE04, FE08 and BSF-CL on the reduction of chicken manure by single strain fermentation broth, Table 4
TABLE 4 influence of addition of Individual microorganisms on the reduction of Chicken manure
As can be seen from Table 4, the results of the two repeated experiments have similar trends, and compared with the blank control group, the differences of the effects of different microorganisms on the reduction rate of the chicken manure are large, and FE01, FE04, FE08 and BSF-CL can obviously increase the reduction rate of the chicken manure.
Therefore, the fermentation liquid of each of FE01, FE04, FE08 and BSF-CL single strains can improve the survival rate of the soldier flies, promote the transformation and weight increment of the soldier flies and promote the transformation of chicken manure. Among them, the BSF-CL strain is the most effective.
Example 3:
a compound microbial preparation for promoting growth of Hermetia illucens larva comprises FE01, FE04, FE08 and BSF-CL.
In this example, 9 groups of complex microbial inocula were prepared, and the specific formula was as follows (V/V):
the concentrations of FE01, FE04, FE08 and BSF-CL fermentation broths were all adjusted to 1X 108Each/ml of the composition is provided with 9 compound combinations, and the compound proportion is as follows:
group 1: FE 01: FE 04: FE 08: BSF-CL ═ 1:1:1:1
Group 2: FE 01: FE 04: FE 08: BSF-CL ═ 2:1:1:1
Group 3: FE 01: FE 04: FE 08: BSF-CL ═ 4:1:1:1
Group 4: FE 01: FE 04: FE 08: BSF-CL ═ 1:2:1
Group 5: FE 01: FE 04: FE 08: BSF-CL ═ 1:4:1
Group 6: FE 01: FE 04: FE 08: BSF-CL ═ 1:1:2:1
Group 7: FE 01: FE 04: FE 08: BSF-CL ═ 1:1:4:1
Group 8: FE 01: FE 04: FE 08: BSF-CL ═ 1:1:1:2
Group 9: FE 01: FE 04: FE 08: BSF-CL ═ 1:1:1:4
Used in the following examples.
Example 4:
the application of the complex microbial inoculum comprises the following application processes:
blank control was 500g of sterilized substrate; the treatment group was 500g of sterilized substrate and 1ml of the complex microbial inoculum prepared in example 3 was added for nine treatment groups.
TABLE 5 growth promoting and transformation promoting effects of different proportions of compound bacteria on stratiomyiid
After the matrix is uniformly stirred, 500 healthy 6-day-old hermetia illucens larvae with similar sizes are simultaneously inoculated into different treatment groups, and each treatment is repeated for 3 times.
After 50% of stratiomyiid prepupa, stratiomyiid is separated from the matrix, and the total dry weight of the stratiomyiid and the dry weight of the residual matrix are counted after the stratiomyiid is dried at 50 ℃. The experimental results were counted using SPSS18.0 statistical analysis software. The results are shown in Table 5.
The survival rate (%) of the soldier fly is equal to the number of transformed soldier flies/the number of soldier flies added before transformation is multiplied by 100 percent
The percent weight gain (%) of the insect (dry weight of hermetia illucens in the experimental group after transformation-dry weight of hermetia illucens in the control group after transformation)/dry weight of hermetia illucens in the control group after transformation
(ii) stratiomyiid conversion (%) - (dry weight of stratiomyiid after transformation-dry weight of stratiomyiid before transformation)/dry weight of chicken manure before transformation × 100%
The chicken manure reduction (%) - (dry weight of chicken manure before transformation-dry weight of chicken manure after transformation)/dry weight of chicken manure before transformation x 100%.
The compound microbial inoculum has a promoting effect on growth and transformation of the stratiomyiid larvae, is added into a chicken manure treatment system of the stratiomyiid larvae, and is used for jointly transforming chicken manure with the stratiomyiid larvae.
As can be seen from the results in Table 5, the optimal ratio of the compound microbial inoculum is as follows: FE 01: FE 04: FE 08: BSF-CL is 4:1:1:1, the weight gain of the flufly is improved by 28.57% compared with that of a flufly chicken manure transformation system without bacteria, and more flufly larva biomass can be obtained.
Claims (4)
1. A composite microbial inoculum for promoting the growth of hermetia illucens larvae comprises: the preservation number of the Corkeslea Kocuria marina FE01 is CCTCC NO: m2015558, the preservation number of Lysinibacillus boronolans FE04 is CCTCC NO: m2015559, Proteus mirabilis (Proteus mirabilis) FE08 preservation number is CCTCC NO: m2015560 and Bacillus subtilis BSF-CL with preservation numbers of CCTCC NO: m2014222;
the compound microbial inoculum is as follows: kocuria (Kocuria marina) FE 01: lisibacillus boronolans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis bsf-CL 4:1:1: 1;
or Kocuria marina (Kocuria marina) FE 01: lysinibacillus boronitolerans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis BSF-CL ═ 2:1:1: 1;
or Kocuria marina (Kocuria marina) FE 01: lysinibacillus boronitolerans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis BSF-CL ═ 1:2:1: 1;
or Kocuria marina (Kocuria marina) FE 01: lysinibacillus boronitolerans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis BSF-CL ═ 1:4:1: 1;
or Kocuria marina (Kocuria marina) FE 01: lysinibacillus boronitolerans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis BSF-CL ═ 1:1:2: 1;
or Kocuria marina (Kocuria marina) FE 01: lysinibacillus boronitolerans FE 04: proteus mirabilis (Proteus mirabilis) FE 08: bacillus subtilis BSF-CL ═ 1: 1:1:2.
2. The use of the complex microbial inoculant of claim 1 in the preparation of a medicament for promoting the growth of hermetia illucens larvae.
3. The use of the complex microbial inoculum of claim 1 in the preparation of a drug for transforming feces.
4. The use of the complex microbial inoculant of claim 1 in the preparation of a medicament for improving the survival rate of soldier flies.
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| CN109258579A (en) * | 2018-11-30 | 2019-01-25 | 湖南农业大学 | A kind of efficient method for breeding that black soldier flies yield is improved using Animal fecal pollution |
| CN110591962B (en) * | 2019-09-28 | 2020-11-06 | 华中农业大学 | Proteus mirabilis BSFLG-CIP5 derived from stratiomyiid intestinal tract and application thereof |
| CN111100806B (en) * | 2019-10-11 | 2022-12-02 | 海南大学 | Areca-nut root rot bactericide prepared by taking boron-resistant lysine bacillus as underpan cells |
| FR3106725B1 (en) | 2020-01-31 | 2022-08-05 | Nolivade | Composition of microorganisms for the breeding of insects |
| FR3106724B1 (en) | 2020-01-31 | 2022-08-05 | Nolivade | Composition for improving insect production |
| CN111172075B (en) * | 2020-02-03 | 2022-02-18 | 南京工业大学 | Hermetia illucens compound microbial preparation, preparation method thereof and application thereof in bean dreg conversion |
| CN112795501B (en) * | 2020-12-22 | 2021-12-14 | 华中农业大学 | Bacillus beiLeisi D2406 separated from stratiomyiid intestinal tract and application thereof |
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