CN113046085A - Animal enzyme for saline-alkali soil improvement and preparation method thereof - Google Patents
Animal enzyme for saline-alkali soil improvement and preparation method thereof Download PDFInfo
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- CN113046085A CN113046085A CN202110331874.5A CN202110331874A CN113046085A CN 113046085 A CN113046085 A CN 113046085A CN 202110331874 A CN202110331874 A CN 202110331874A CN 113046085 A CN113046085 A CN 113046085A
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- 238000011282 treatment Methods 0.000 claims description 37
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- NCYVXEGFNDZQCU-UHFFFAOYSA-N nikethamide Chemical compound CCN(CC)C(=O)C1=CC=CN=C1 NCYVXEGFNDZQCU-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
Abstract
The invention discloses an animal enzyme for saline-alkali soil improvement, which is prepared from the following components in parts by weight: 60-100 parts of animal protein raw material, 0.1-0.5 part of complex enzyme preparation, 0.1-0.5 part of zymophyte agent A, 0.1-1 part of zymophyte agent B and 1-2 parts of auxiliary material; simultaneously discloses a preparation method and application of the animal ferment for improving the saline-alkali soil. The production process is simple and reasonable, the raw materials are easy to obtain, the produced product has remarkable functionality, the improvement effect on saline-alkali soil is good, and the utilization field of the cultivation waste is widened.
Description
Technical Field
The invention belongs to the technical field of bioengineering, and particularly relates to an animal ferment for saline-alkali soil improvement and a preparation method thereof.
Background
The problem of soil salinization is a worldwide problem, causes great harm to land utilization in China and seriously hinders the improvement of land capability. In addition, the saline-alkali soil treatment measures are incomplete and the treatment technology is not comprehensive, so that the area of the saline-alkali soil tends to increase year by year. Because the saline-alkali soil is not suitable for crop cultivation, a large amount of cultivated land area is idle, and other modes can not be utilized through short-term transformation, thereby causing great threats to food safety and reasonable utilization of land resources in China. The salinized soil in China has wide distribution range, is mainly in northeast, northwest, north China and coastal areas, and has a total area of more than 3000 ten thousand hm2And occupies 10 percent or more of the total cultivated land area, and a large amount of cultivated land is abandoned or even abandoned every year along with the salinization of soil. The cultivated land resource is one of the most important resources in China, is a basic condition for guaranteeing food production and food safety in China, and causes serious influence and harm to agricultural production due to soil salinization.
However, China is also a big livestock breeding country, more than 400 million tons of animals died of illness are generated in China every year, the animal carcasses are generally treated by traditional treatment modes such as landfill, incineration, high-temperature treatment and the like, but the treatment modes have the characteristics of environmental pollution, high treatment cost, low product added value and the like, and the resources such as protein, fat and the like cannot be fully utilized, so that a new treatment mode needs to be found, and the application range of the materials is expanded.
Disclosure of Invention
The first technical problem to be solved by the invention is as follows: the animal ferment prepared by the method for improving the saline-alkali soil is provided, and the effect of improving the saline-alkali soil by the animal ferment is obvious.
The second technical problem to be solved by the invention is: the preparation method of the animal enzyme for improving the saline-alkali soil is characterized by comprising the steps of emulsifying, sterilizing and carrying out enzymolysis on animals died of diseases, then adding microorganisms for anaerobic fermentation and after-ripening treatment, and adding auxiliary materials.
In order to solve the technical problems, the technical scheme of the invention is as follows:
an animal enzyme for saline-alkali soil improvement is prepared from the following components in parts by weight:
60-100 parts of animal protein raw material, 0.1-0.5 part of complex enzyme preparation, 0.1-0.5 part of zymophyte agent A, 0.1-1 part of zymophyte agent B and 1-2 parts of auxiliary material.
Preferably, the animal ferment for saline-alkali soil improvement comprises the following components in parts by weight: 80 parts of animal protein raw material, 0.3 part of complex enzyme preparation, 0.3 part of zymophyte agent A, 0.6 part of zymophyte agent B and 1.5 parts of auxiliary material.
Preferably, the animal protein raw material is slaughter leftovers and farmed animals died of diseases; farm animals include poultry, livestock and pets.
Further, the animal protein raw materials are leftovers from slaughtering chicken intestines, duck half shells and the like, fish impurities and other aquatic product processing leftovers, dead pigs, dead chickens, dead cattle and other dead animal bodies.
Preferably, the compound enzyme preparation comprises 0.1-1 part by weight of acid protease and 0.1-1 part by weight of lipase; the fermentation inoculant A comprises 0.1-1 part by weight of bacillus subtilis, 0.1-1 part by weight of bacillus licheniformis and 0.5-2 parts by weight of saccharomyces cerevisiae; the fermentation inoculum B is 0.1-1 part of enterococcus faecalis, 0.1-2 parts of lactobacillus plantarum, 0.1-0.5 part of lactobacillus acidophilus and 0.1-1 part of streptococcus thermophilus respectively in parts by weight; the adjuvants include one or more of glucose, corn flour, and molasses.
The acidic protease is prepared by fermenting, refining and refining microorganisms, and can effectively hydrolyze protein under the condition of low pH.
Lipase is prepared by fermenting and refining microorganisms, and can convert fat into glycerol and fatty acid.
The bacillus subtilis is aerobic bacteria, has high growth speed and low requirement on nutrition, and can efficiently secrete a plurality of enzymes for degradation; active substances such as subtilin, polymyxin, nystatin, gramicidin and the like are secreted, and the active substances have obvious inhibiting effect on pathogenic bacteria or pathogenic bacteria with endogenous infection.
The bacillus licheniformis is facultative anaerobe, can consume oxygen in the growth process, has a plurality of excellent characteristics of heat resistance, rich enzyme system, higher enzyme yield, safety and the like, and has the characteristics of high temperature resistance, drying resistance, acid resistance, cholate resistance, artificial gastric juice resistance and the like.
The saccharomyces cerevisiae belongs to facultative anaerobe, needs a large amount of oxygen during propagation, contains rich beta-1, 3-glucan and mannan oligosaccharide in cell walls, contains rich small peptide and nucleic acid in yeast cell protoplasm, produces bouquet by fermentation, and improves the palatability of fermented materials.
Enterococcus faecalis, a kind of lactic acid bacteria, belongs to enterococcus of streptococcaceae, is facultative anaerobic, grows well under aerobic conditions, has good heat resistance, and has a tolerance pH range of 4.0-9.6. The acid and gas are produced, the L-lactic acid can be secreted, and the nitrogen-free extract of various carbohydrates can be converted into lactic acid which can be completely absorbed and utilized by organisms. Can produce Volgamycin and effectively inhibit the growth and the propagation of listeria, staphylococcus aureus and putrefying microorganisms.
Lactobacillus plantarum and facultative anaerobe can grow at the optimum temperature of 30-35 ℃ in the pH range of 4.5-9.5, grow well in an anaerobic environment, produce lactic acid, have high acid resistance, and can produce various metabolites with special functions, such as organic acid, polypeptide lactobacillus plantarum and the like.
Lactobacillus acidophilus, belonging to the genus lactobacillus of the family lactobacillus, anaerobic or facultative anaerobic; it can utilize glucose, fructose, lactose and cane sugar to make homotype fermentation, and can produce DL-type lactic acid by means of fermentation. Has effects in inhibiting growth of helicobacter pylori, Staphylococcus aureus, Salmonella, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa; stimulating immune system and improving immunity.
The streptococcus thermophilus belongs to the streptococcus genus, and is facultative anaerobic bacteria or microaerobic, and has good heat resistance; the optimum pH is 6.0-7.0, the protease activity in the body of Streptococcus thermophilus is usually weak or even lack of necessary protease, so the most suitable nitrogen source is protein hydrolysate.
A preparation method of animal ferment for saline-alkali soil improvement comprises the following steps:
a. putting animal source raw materials into a primary crushing device, and performing primary crushing treatment, wherein the crushed materials reach 10-20 meshes; then conveying the mixture to a mincing device for secondary crushing treatment, and crushing the mixture to 100-200 meshes;
b. b, pulping and sterilizing the material prepared in the step a;
c. adding 1-2% by mass of auxiliary materials into the material prepared in the step b, stirring and cooling to 40-60 ℃;
d. adding 0.1-0.5% of a complex enzyme preparation into the material in the step c, stirring for enzymolysis for 1-3 hours, wherein the stirring frequency is 5 r/min; then adjusting the pH value to 6.0-7.0;
e. inoculating 0.1-0.5% of a fermentation inoculum A into the slurry prepared in the step d, and carrying out aerobic fermentation, wherein the stirring frequency is 5r/min, 0.4-0.6 VVm of air is introduced, and the fermentation time is 12-24 hours;
f. e, inoculating 0.1-1% of a fermentation microbial inoculum B into the slurry prepared in the step e, and carrying out anaerobic fermentation at the set temperature of 30-35 ℃ for 24 hours at 6-hour intervals by stirring for 1 hour;
g. conveying the slurry prepared in the step f into a sealed container for after-ripening for 3-5 days, wherein the fermentation temperature is 30-35 ℃, and an exhaust port is arranged at the top of the container for exhausting;
h. and g, crushing the slurry prepared in the step g again, filtering the slurry by a sieve of 100-200 meshes, and filling the filtered slurry to obtain the liquid animal enzyme for improving the saline-alkali soil, wherein the solid animal enzyme for improving the saline-alkali soil can be obtained by drying the liquid animal enzyme for improving the saline-alkali soil.
Preferably, the sterilization temperature in the step b is 80-100 ℃, the sterilization time is 0.5-3 hours, the stirring is continuously carried out in the sterilization process, and the stirring frequency is 1-5 r/min.
An animal ferment for improving saline-alkali soil is prepared by the above preparation method, is liquid or solid, contains compound probiotic bacteria such as lactobacillus, spore bacteria, and yeast, and has total viable bacteria amount of 1 × 104～1×109cfu/ml, and the content of small peptide is 10-30% (based on dry basis), and the method can be used for saline-alkali soil improvement and assisting in development of saline-alkali soil agriculture.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the enzyme prepared by the invention is rich in organic matters, effectively solves the problem of lack of organic matters in saline-alkali soil, and is suitable for improvement of the saline-alkali soil.
2. The enzyme prepared by the invention is rich in organic acid, has low pH value, can neutralize alkaline substances in saline-alkali soil, effectively reduces the pH value of the saline-alkali soil, and is suitable for improving the saline-alkali soil.
3. According to the invention, animal source protein raw materials such as animals died of illness and the like are converted into the animal enzyme for improving the saline-alkali soil with acid aroma or bouquet through biological fermentation, the product is rich in various probiotics and metabolites thereof, such as biological antibacterial peptide, probiotics, biological stimulin and other functional substances, the salt and alkali resistance of the plant can be improved while nutrient substances are provided for the plant, and the animal enzyme can be used as a special fertilizer for agricultural planting of the saline-alkali soil to promote the growth of the plant.
4. The method of the invention treats animals died of illness, and the obtained treated product is stored in a sealed way in high-temperature seasons, and can be stored and preserved for a long time. Provides a biological preservation technology completely different from cold-chain logistics and chemical preservation for the preservation and preservation of animal protein, and can greatly reduce the investment cost and energy consumption cost of the storage and transportation of the cold-chain logistics of meat products.
5. The invention has better environmental protection advantage and high-value utilization advantage, can realize the harmless treatment of animals died of illness, provides a set of new high-value utilization approach for the high-value utilization of the animals died of illness, and realizes the change of waste into valuable.
6. The traditional treatment process has the characteristics of environmental pollution, high treatment cost and low added value of products, which is the fundamental reason for the difficulty in running the projects at present. The invention can carry out harmless treatment and resource utilization on animals died of diseases, and solves the problems.
In a word, the production process is simple and reasonable, raw materials are easy to obtain, the produced product has obvious functionality, the improvement effect on saline-alkali soil is good, and the utilization field of the breeding waste is widened.
Detailed Description
The invention is further illustrated by the following examples.
Example one
1000kg of dead chickens are put into a crushing device for crushing treatment, and the materials are crushed to be below 20 meshes. And then conveying the materials to a mincing device for mincing treatment, and mincing to below 100 meshes. And (4) sterilizing the minced materials at the temperature of 80 ℃ for 1 hour. Stirring is continuously carried out in the sterilization process, and the stirring frequency is 5 r/min. Adding 2% by mass of glucose into the sterilized materials, and cooling to 55 ℃. And after the temperature reduction is finished, adding 0.3% of complex enzyme preparation (0.4 part of acid protease and 0.6 part of lipase), stirring and performing enzymolysis for 3 hours, wherein the stirring frequency is 5r/min, and the pH value is adjusted to be within the range of 6.0-7.0. And (2) conveying the slurry prepared in the step (a) into an aerobic fermentation container, inoculating 0.1% of fermentation broth A agent (0.6 part of bacillus subtilis, 0.6 part of bacillus licheniformis and 0.8 part of saccharomyces cerevisiae), stirring at the frequency of 5r/min, introducing 0.4VVm of air, and fermenting for 24 hours. After the aerobic fermentation is finished, the mixture is conveyed to an anaerobic fermentation container, 0.1% of fermentation inoculum B (0.3 part of enterococcus faecalis, 1.4 parts of lactobacillus plantarum, 0.4 part of lactobacillus acidophilus and 0.6 part of streptococcus thermophilus) is inoculated, the temperature is set at 30 ℃, the mixture is stirred for 1 hour at intervals of 6 hours, and the mixture is conveyed to a sealed storage tank for after-ripening for 5 days after 24 hours of anaerobic fermentation. And (4) conveying the slurry after the post-maturation to a grinding device for grinding treatment, and filling to prepare the animal enzyme for improving the saline-alkali soil. The detection results are shown in table 1:
TABLE 1 detection data for animal enzymes for saline and alkaline land improvement
Example two
1000kg of pigs died of illness are put into a crushing device for crushing treatment, and the materials are crushed to be below 20 meshes. And then conveying the materials to a mincing device for mincing treatment, and mincing to below 100 meshes. And (4) sterilizing the minced materials at the temperature of 80 ℃ for 1 hour. Stirring is continuously carried out in the sterilization process, and the stirring frequency is 5 r/min. Adding 2% by mass of glucose into the sterilized materials, and cooling to 55 ℃. And after the temperature reduction is finished, adding 0.3% of complex enzyme preparation (0.4 part of acid protease and 0.6 part of lipase), stirring and performing enzymolysis for 3 hours, wherein the stirring frequency is 5r/min, and the pH value is adjusted to be within the range of 6.0-7.0. And (2) conveying the slurry prepared in the step (a) into an aerobic fermentation container, inoculating 0.1% of fermentation broth A agent (0.6 part of bacillus subtilis, 0.6 part of bacillus licheniformis and 0.8 part of saccharomyces cerevisiae), stirring at the frequency of 5r/min, introducing 0.4VVm of air, and fermenting for 24 hours. After the aerobic fermentation is finished, the mixture is conveyed to an anaerobic fermentation container, 0.1% of fermentation inoculum B (0.3 part of enterococcus faecalis, 1.4 parts of lactobacillus plantarum, 0.4 part of lactobacillus acidophilus and 0.6 part of streptococcus thermophilus) is inoculated, the temperature is set at 30 ℃, the mixture is stirred for 1 hour at intervals of 6 hours, and the mixture is conveyed to a sealed storage tank for after-ripening for 5 days after 24 hours of anaerobic fermentation. And (4) conveying the slurry after the post-maturation to a grinding device for grinding treatment, and filling to prepare the animal enzyme for improving the saline-alkali soil. The detection results are shown in table 2:
TABLE 2 detection data for animal enzymes for saline and alkaline land improvement
Experimental example 1
The animal ferment for saline-alkali soil improvement prepared in the first embodiment is hermetically packaged in a plastic barrel for 12 barrels, two barrels are opened every 2 months for sampling detection, the pH, the salmonella, the escherichia coli and the staphylococcus aureus are detected, the average value of the two barrels is taken as the detection result, and the result is shown in table 3.
TABLE 3 preservation test data for animal enzymes for saline and alkaline land improvement
| Time | 2 month | 4 month | 6 month | 8 month | 10 month | 12 month |
| pH value | 4.8 | 4.8 | 4.7 | 4.6 | 4.6 | 4.6 |
| Salmonella (25 ml per) | Not detected out | Not detected out | Not detected out | Not detected out | Not detected out | Not detected out |
| Escherichia coli (cfu/ml) | ＜10 | ＜10 | ＜10 | ＜10 | ＜10 | ＜10 |
| Staphylococcus aureus (cfu/ml) | 0 | 0 | 0 | 0 | 0 | 0 |
Experimental example twoSaline-alkali soil improvement effect experiment I
Randomly selecting 20 green radishes with similar shapes and sizes, and only taking the positions of the radish heads. In an experimental group, 10 radish heads are diluted by 1 kg of the animal ferment for saline-alkali soil improvement prepared in the embodiment I with 500 kg of water, and are soaked in 5% saline water for 1 day, and the number of test days is 4. The 10 radish heads of the control group were soaked in 5% saline without treatment for 4 days. Results 9 radish heads in the test group have sprouting growth, and the control group has all atrophy and softness without growth signs.
Experimental example IIISaline-alkali soil improvement effect experiment 2
The fertilizer efficiency test was carried out on the organic fertilizer prepared in the above example.
The test was set up with the following treatments:
treatment A1, namely using the organic fertilizer prepared in example 1 as a leaf-eating grass base fertilizer with the use amount of 40Kg/667m2；
Treatment A2, namely using the organic fertilizer prepared in example 2 as a leaf-eating grass base fertilizer with the use amount of 40Kg/667m2；
B, using the common organic fertilizer produced by the Shouguanyuan organic fertilizer processing factory as a leaf-eating grass base fertilizer with the use amount of 40Kg/667m2
Treatment of C, without using organic fertilizer
The test plots are located in a planting area of Shandong Baide agriculture and animal husbandry science and technology Limited, the soil type of the area belongs to the type of coastal saline-alkali soil, 4 plots with one mu are divided flatly in 5 months in 2020, and the plots are respectively sown with the leaf-eating grass after the treatments of A1, A2, B and C. During the period, other management modes are the same, and the results of the test (as base fertilizer) of the comparative organic fertilizer on the leaf-eating grass are carried out after harvesting:
the comparative experiments show that the yield of the leaf-eating grass applied with the animal ferment (used as a base fertilizer) is increased compared with the leaf-eating grass applied with the common organic fertilizer (used as a base fertilizer) by the following steps: the treatment A1 increased 8.1% more than the treatment B, and the treatment A2 increased 10.3% more than the treatment B; furthermore, the leaf-eating grass has soft texture, softness and juiciness and better palatability. Meanwhile, the treatment B showed a 31.3% increase over the treatment C. The data on the saline-alkali soil improvement are shown in table 4:
TABLE 4 percentage increase and decrease of saline-alkali soil after different treatments compared with original soil
| Reduction of pH | Reduced salt content | Water holding capacity increasing device | Organic matterIncrease | Increased nitrogen content | Quick-acting phosphorus increasing agent | Quick-acting potassium increasing agent | |
| Process A1 | 12.73％ | 35.26％ | 58.11％ | 48.5％ | 91.8％ | 64.6％ | 77.2％ |
| Process A2 | 13.25％ | 35.58％ | 59.04％ | 54.7％ | 92.6％ | 67.1％ | 78.9％ |
| Treatment B | 10.44％ | 23.37％ | 35.53％ | 32.2％ | 78.6％ | 49.1％ | 27.5％ |
| Treatment C | 1.57％ | 9.98％ | 17.73％ | 4.1％ | -3.2％ | -5.8％ | -8.5％ |
The data show that the invention has obvious effect on improving saline-alkali soil and can promote the yield of the planted corresponding plants.
It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
Claims (7)
1. The animal enzyme for improving the saline-alkali soil is characterized by being prepared from the following components in parts by weight:
60-100 parts of animal protein raw material, 0.1-0.5 part of complex enzyme preparation, 0.1-0.5 part of zymophyte agent A, 0.1-1 part of zymophyte agent B and 1-2 parts of auxiliary material.
2. The animal ferment for saline-alkali soil improvement according to claim 1, wherein: the animal ferment for improving the saline-alkali soil comprises the following components in parts by weight: 80 parts of animal protein raw material, 0.3 part of complex enzyme preparation, 0.3 part of zymophyte agent A, 0.6 part of zymophyte agent B and 1.5 parts of auxiliary material.
3. The animal ferment for saline-alkali soil improvement according to claim 1, wherein: the animal protein raw material is slaughter leftovers and breeding animals died of diseases; the breeding animals include poultry, livestock and pets.
4. The animal ferment for saline-alkali soil improvement according to claim 1, wherein: the compound enzyme preparation comprises 0.1-1 part by weight of acid protease and 0.1-1 part by weight of lipase; the fermentation inoculant A comprises 0.1-1 part by weight of bacillus subtilis, 0.1-1 part by weight of bacillus licheniformis and 0.5-2 parts by weight of saccharomyces cerevisiae; the fermentation inoculum B is 0.1-1 part of enterococcus faecalis, 0.1-2 parts of lactobacillus plantarum, 0.1-0.5 part of lactobacillus acidophilus and 0.1-1 part of streptococcus thermophilus respectively in parts by weight; the adjuvants include one or more of glucose, corn flour, and molasses.
5. The preparation method of the animal ferment for improving the saline-alkali soil is characterized by comprising the following steps of:
a. putting animal source raw materials into a primary crushing device, and performing primary crushing treatment, wherein the crushed materials reach 10-20 meshes; then conveying the mixture to a mincing device for secondary crushing treatment, and crushing the mixture to 100-200 meshes;
b. b, pulping and sterilizing the material prepared in the step a;
c. adding 1-2% by mass of auxiliary materials into the material prepared in the step b, stirring and cooling to 40-60 ℃;
d. adding 0.1-0.5% of a complex enzyme preparation into the material in the step c, stirring for enzymolysis for 1-3 hours, wherein the stirring frequency is 5 r/min; then adjusting the pH value to 6.0-7.0;
e. inoculating 0.1-0.5% of a fermentation inoculum A into the slurry prepared in the step d, and carrying out aerobic fermentation, wherein the stirring frequency is 5r/min, 0.4-0.6 VVm of air is introduced, and the fermentation time is 12-24 hours;
f. e, inoculating 0.1-1% of a fermentation microbial inoculum B into the slurry prepared in the step e, and carrying out anaerobic fermentation at the set temperature of 30-35 ℃ for 24 hours at 6-hour intervals by stirring for 1 hour;
g. conveying the slurry prepared in the step f into a sealed container for after-ripening for 3-5 days, wherein the fermentation temperature is 30-35 ℃, and an exhaust port is arranged at the top of the container for exhausting;
h. and g, crushing the slurry prepared in the step g again, filtering the slurry by a sieve of 100-200 meshes, and filling the filtered slurry to obtain the liquid animal enzyme for improving the saline-alkali soil, wherein the solid animal enzyme for improving the saline-alkali soil can be obtained by drying the liquid animal enzyme for improving the saline-alkali soil.
6. The method for preparing animal ferment for saline-alkali soil improvement according to claim 5, wherein the animal ferment comprises the following components: and c, sterilizing at 80-100 ℃ for 0.5-3 hours in the step b, and continuously stirring in the sterilizing process, wherein the stirring frequency is 1-5 r/min.
7. The application of the animal enzyme for improving the saline-alkali soil is characterized in that: the animal ferment for saline-alkali soil improvement is applied to saline-alkali soil improvement.
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