CN107058170A - Pediococcus acidilactici BCC 1 and its application of the one plant height effect using xylo-oligosaccharide - Google Patents

Pediococcus acidilactici BCC 1 and its application of the one plant height effect using xylo-oligosaccharide Download PDF

Info

Publication number
CN107058170A
CN107058170A CN201710074474.4A CN201710074474A CN107058170A CN 107058170 A CN107058170 A CN 107058170A CN 201710074474 A CN201710074474 A CN 201710074474A CN 107058170 A CN107058170 A CN 107058170A
Authority
CN
China
Prior art keywords
pediococcus acidilactici
bcc
probiotics
feed
xos
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710074474.4A
Other languages
Chinese (zh)
Other versions
CN107058170B (en
Inventor
袁建敏
雷钊
呙于明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China Agricultural University
Original Assignee
China Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China Agricultural University filed Critical China Agricultural University
Priority to CN201710074474.4A priority Critical patent/CN107058170B/en
Publication of CN107058170A publication Critical patent/CN107058170A/en
Application granted granted Critical
Publication of CN107058170B publication Critical patent/CN107058170B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Abstract

The Pediococcus acidilactici BCC 1 using xylo-oligosaccharide and its application the present invention relates to plant height effect.The present invention is for easily there is Pediococcus acidilactici BCC 1 and its application of the intestinal health problem there is provided plant height effect using xylo-oligosaccharide during modern Production of Livestock and Poultry;The present invention also provides one kind and constitutes feed addictive by Pediococcus acidilactici (Pediococcus acidilactici) BCC 1 and xylo-oligosaccharide, the product can further improve the application effect of lactic acid bacteria, improve the intestinal tissue form of poultry anterior intestine, improve the content of caecum Short-Chain Fatty Acids, and reduce caecum pH value, improve the intestinal health of poultry, greatly improve the production performance of poultry, realize that green and healthy are cultivated.

Description

Pediococcus acidilactici BCC-1 and its application of the one plant height effect using xylo-oligosaccharide
Technical field
The invention belongs to livestock and poultry cultivation and feed-formulating technique field, and in particular to one plant of Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 and its application.
Background technology
China is livestock and poultry cultivation big country, with the development of China's aquaculture, needs to consume substantial amounts of mixed feed every year. China's Feed Manufacturing amount reaches 1.9 ten thousand tons within 2016.Feedstuff corn, the energy feed such as wheat contain substantial amounts of anti-nutrition because Sub- xylan (arabinoxylan, AX).Xylan enter livestock and poultry body in after can form viscous chyme, cause pathogen propagation and The intestinal health problem such as intestinal inflammation, reduces the utilization of nutrient, and then reduce the production performance of livestock and poultry.Therefore, wood is eliminated Negative effect of the glycan to livestock and poultry, improves animal and bird intestines health problem, is always problem of concern.Further, since everybody To antibiotic residue attention rate more and more higher, Production of Livestock and Poultry develops to disabling antibiotic direction, finds the addition of novel green feed Agent substitute antibiotics are main development directions from now on.At present, the intestinal health of livestock and poultry is improved by adding probiotics, More widespread practice is become.
Pediococcus acidilactici is as one kind of lactic acid bacteria, and it can secrete pediocin, so as to suppress the increasing of many pathogens Grow.At present, the application study report on Pediococcus acidilactici on livestock and poultry is less, especially on broiler chicken, also lacks related report Road.XOS is a kind of common prebiotics, can be effectively promoted the propagation of beneficial bacteria of intestinal tract.Therefore, filtering out can be efficiently sharp The Pediococcus acidilactici bred with XOS, uses with XOS compatibilities, can reach the probiotic effects of Synergistic, preferably adjust enteron aisle Health, so that promoting the production performance of livestock and poultry improves.
The content of the invention
For above-mentioned technical problem, the present invention seeks to the intestinal health problem existed for livestock raised for meat fowl in breeding process, Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 and its application of the one plant height effect using xylo-oligosaccharide are provided; The present invention also provides a kind of composite feed additive using Pediococcus acidilactici BCC-1 and XOS as main active, to improve The intestinal health of livestock and poultry, so as to improve the production performance of livestock and poultry.
Realize that the object of the invention technical scheme is as follows:
One plant of Pediococcus acidilactici (Pediococcus acidilactici) BCC-1, is from white meat-type chickens caecum chyme Isolated Pedicoccus acidilacticii strain, it is determined by colony morphological observation, physiological and biochemical property, molecular biology identification etc. For Pediococcus acidilactici.The bacterial strain is the advantage lactic acid bacteria of white meat-type chickens caecum, with the efficient effect bred using xylo-oligosaccharide.
Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 that the present invention is provided is November 4 in 2016 Day is preserved in China General Microbiological DSMZ, and (abbreviation CGMCC, address is:Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3 Institute of Microorganism, Academia Sinica, postcode:100101), deposit number is CGMCC NO.13242, Classification And Nomenclature For Pediococcus acidilactici (Pediococcus acidilactici).
Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 that the present invention is provided has following biological characteristics Levy:Colony colour is milky, and surface is smooth, circular protrusions.It is purple after Gram's staining.It is to life under an electron microscope Long, single growth is more rare.
Known array in bacterial strain BCC-1 16S rDNA sequence and GenBank is carried out into Blast to compare, from database The middle 16S rDNA for obtaining related kind, phylogenetic tree construction, and by the cellular morphology of strain, physiological and biochemical property and The experimental datas such as 16S rDNA sequences, which are integrated, determines that the bacterial strain belongs to Pediococcus acidilactici category, is ultimately determined to Pediococcus acidilactici (Pediococcus acidilactici)。
Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 that the present invention is provided, which has, efficiently utilizes XOS The characteristics of to be bred.
Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 of the present invention can be carried out using MRS culture mediums Culture.Typically use Anaerobic culturel;Temperature is generally 37 DEG C during quiescent culture.
The present invention also provides Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 cultural method, including Fermented and cultured is carried out using MRS culture mediums, fermentation condition is 30 DEG C, and rotating speed is 200rpm, be 6.5, anaerobism with ammoniacal liquor regulation pH Ferment 24h;The sucrose solution that 200mL mass fractions are 30% is added after fermentation 8h in every 3L nutrient solutions, Pediococcus acidilactici is made Zymotic fluid.
The present invention also provides the Tiny ecosystem system prepared by Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 Agent.
Preferably, the active component of the probiotics includes Pediococcus acidilactici (Pediococcus Acidilactici) BCC-1 and XOS (xylo-oligosaccharide).
Present invention research finds that Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 and XOS compatibilities make With can reach the probiotic effects of Synergistic, preferably adjust intestinal health, so as to promote the production performance of animal.
It is further preferred that Pediococcus acidilactici BCC-1 content is 1-2 × 1010cfu/g in the probiotics; XOS mass fraction is 45-47.5%.
It is further preferred that Pediococcus acidilactici BCC-1 content is 1 × 1010cfu/g in the probiotics; XOS mass fraction is 45%.
Its raw material of the probiotics also includes also including diluent, and the diluent is preferably starch.Preferably, institute State XOS purity >=90%.Usually, the XOS can be prepared by prior art conventional method, for example by corncob hydrolyze and Come;Can also be commercially available.
Probiotics of the present invention may be used as the special composite feed additive of Wheat Based Diet, especially as poultry The special composite feed additive of fowl Wheat Based Diet, to improve the intestinal health of livestock and poultry, so as to improve the production performance of livestock and poultry.
Preferably, addition of the probiotics of the present invention in the special mixed feed of Wheat Based Diet is 0.01% (mass fraction).
The present invention also provides the preparation method of above-mentioned probiotics, including each raw material is mixed by prior art conventional method Conjunction is uniformly produced.
Preferably, the preparation method of above-mentioned probiotics, comprises the following steps:By Pediococcus acidilactici BCC-1 fermentation Liquid is added after starch after mixing, and bacterium powder is made in low temperature spray drying;By gained bacterium powder and XOS in proportion (1:1) it is well mixed, Produce.
Specifically, the preparation method of above-mentioned probiotics, comprises the following steps:
1) after the seed liquor that Pediococcus acidilactici BCC-1 is prepared with MRS culture mediums, liquid is carried out according to 1% inoculum concentration inoculation Body fermented and cultured;
It is preferred that by after actication of culture 2 times, aseptically, choosing the strain in logarithmic phase, being inoculated into fermentation tank In zymotic fluid;
2) culture medium that zymotic fluid is used is MRS culture medium, and fermentation condition is 30 DEG C, and rotating speed is 200rpm, is adjusted with ammoniacal liquor It is 6.5, anaerobic fermentation 24h to save pH, adds the sucrose solution that 200mL mass fractions are 30% after the 8h that ferments in every 3L nutrient solutions;
3) it is accurate to claim fermentation 1L streptococcus acidi lactici fermented solutions, 300g starch is added thereto, after spray drying, is obtained every gram of bacterium powder and is contained Some biodiasmin quantity is 2 × 1010cfu;
4) by the xylo-oligosaccharide of gained lactic acid bacteria bacterium powder and 90% purity according to 1:After the quality such as 1 is mixed, it is made required Compound micro-ecological preparation, viable bacteria concentration is 1 × 1010cfu/g;Xylo-oligosaccharide content is 45%.
Present invention additionally comprises above-mentioned Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 or probiotics Application in Wheat Based Diet feed formulation, especially in application of the livestock and poultry in Wheat Based Diet feed formulation.It is of the present invention Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 and probiotics are applied to feed-processing plant and plant Autogamy feed.It can be well mixed when using with other raw materials in feed, can be in advance by probiotics of the present invention with lacking Measure feed to be well mixed, remix in large quantities of feeds, Direct-fed.
It is recommended that when wheat weighs less than 30% in complete feed, the addition of the probiotics is 0.01%, or Pediococcus acidilactici BCC-1 content 1 × 109cfu/kg, XOS mass fraction are 0.005% in complete feed described in person;When complete When wheat weight is more than 30% in valency feed, the addition of the probiotics is 0.015%, or controls the full price to raise Pediococcus acidilactici BCC-1 content is 1.5 × 1010cfu/g in material, and XOS mass fractions are 0.075%.
Livestock and poultry of the present invention include broiler chicken, laying hen, meat duck, the poultry such as egg duck, and live pig.
Beneficial effect
Present invention is generally directed to the intestinal health problem that meat type chicken breeding process is present, the lactic acid sheet ball filtered out is configured The composite feed additive of bacterium BCC-1 and XOS composition, to improve the intestinal health of broiler chicken, so as to improve the productivity of broiler chicken Energy.
Pediococcus acidilactici, as one kind of lactic acid bacteria, is a kind of important fungal component in Intestine of Broiler.With others breast Sour bacterium is compared, and Pediococcus acidilactici can also be by secreting pediocin, so as to suppress the propagation of many pathogens.In addition, Prebiotics (oligosaccharide) can by it is specific promotion probiotics propagation, to play corresponding prebiotic effect.XOS conducts A kind of new prebiotics, can be used with lactic acid bacteria compatibility, play bigger Synergistic prebiotic effect, adjust the intestines of broiler chicken Road health, improves the production performance of broiler chicken.
Advantages of the present invention:
(1) in Wheat Based Diet, compared with individually addition XOS, the present invention can further improve the production performance of broiler chicken.
(2) in Wheat Based Diet, compared with individually addition Pediococcus acidilactici BCC-1, the present invention is equally further increased The production performance of broiler chicken.
(3) compared with XOS and Pediococcus acidilactici BCC-1 is used alone, the present invention can also further reduce enteron aisle chyme Stickiness and pH value, improve the content of enteron aisle butyric acid, improve the intestinal health of poultry.
Social benefit and economic benefit
1st, main application:Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 of the present invention and Probiotics, the broiler chicken that is particularly suitable for use in, laying hen, meat duck, in the preparation of poultry Wheat Based Diet feed such as egg duck.
2nd, application benefit:Add and matched somebody with somebody by Pediococcus acidilactici BBC-1 and XOS in the daily ration of broiler configured by raw material of wheat The compound additive put so that broiler chicken 21d body weight is improved compared with control group, and 649.18g is brought up to by 621.03g;Feed-weight ratio shows Reduction is write, 1.51 are reduced to by 1.57.Relatively the individually increased weight of addition XOS broiler chicken, 649.18g is increased to from 621.63g;Material weight Than reduction, 1.51 are reduced to from 1.53.Relatively the individually increased weight of addition Pediococcus acidilactici BCC-1 broiler chicken, increases to from 628.56 649.18;Feed-weight ratio is reduced, and 1.51 are reduced to from 1.53.
3rd, promotional value:In recent years, wheat is widely used as energy feed.In 2012-2013, China Mixed feed, pig, poultry, egg fowl consume respectively wheat and its accessory substance 23,390,000,19,860,000,8,370,000,8,630,000 tons.But due to Wheat and its accessory substance are rich in substantial amounts of ANFs, mainly araboxylan, can cause animal and fowl fodder nutrien utilization The reduction of rate, and intestinal health is damaged, so as to reduce the production performance of livestock and poultry.
At present, the producer of China's probiotics there are about more than 400 families, the probiotics for producing and using every year nearly 4000 Ton.According to the current feed market Quantity customizing of China (about 200,000,000 tons of China's feed annual production in 2016), 20,000 tons are about needed every year Probiotics product.And lactic acid bacteria is as one of most commonly seen feeding micro-ecological preparation, therefore with before wide application Scape.It is different from traditional lactic bacteria additive, compound Pediococcus acidilactici additive prepared by the present invention, also comprising XOS, it is in poultry The propagation of lactic acid bacteria, thus more single lactic bacteria additive can further be promoted in carcass, performance that can be more preferable is prebiotic Effect.
Brief description of the drawings
Fig. 1 is the sediments microscope inspection figure after Pediococcus acidilactici BCC-1 is dyed.
Fig. 2 is the influence figure that different carbon source is bred to Pediococcus acidilactici.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.It is unreceipted specific in embodiment Technology or condition person, are carried out according to the technology or condition described by document in the art, or according to product description.It is used Reagent or the unreceipted production firm person of instrument, are the conventional products that can be commercially available by regular distributor.
Pediococcus acidilactici BCC-1 described in following examples, separation and white meat-type chickens caecum chyme, and by Chinese common micro- life Thing DSMZ preservation (CGMCC), preserving number is CGMCC 13242;The XOS of 90% purity can by corncob hydrolysis and Come.
The Pediococcus acidilactici BCC-1 of embodiment 1 separation screening, identification and preservation
1st, Pediococcus acidilactici isolates and purifies:Under aseptic condition, from white plumage meat of the feeding without antibiotic feed of health Isolated chyme in chicken (42 age in days) caecum, is trained using agar MRS culture mediums to chyme supernatant (suitable dilution) Support, select single lactic acid bacteria bacterium colony and purified, each bacterium colony purified for 10 generations.
2nd, the observation of colonial morphology:Bacterium colony is creamy white on agar MRS flat boards, circular, neat in edge, shows to moisten light It is sliding, easily provoke.
3rd, the screening of excellent Pediococcus acidilactici:The glucose in MRS culture mediums is substituted with XOS, at xylose and glucose Reason group is positive control, without the OD that sugar source is measure bacterium solution after negative control, each strain culturing 24h600, choose OD values most Big bacterial strain is identified.
4 Gram's staining:Ferment product is taken, is carried out after Gram's staining, as a result microscopy observation is shown in Fig. 1.
5th, 16S rDNA sequencings:Extract after DNA of bacteria, be sent to Beijing three and win polygala biotechnology Co., Ltd It is sequenced.Bacterial strain BCC-1 16S rDNA sequences are as shown in sequence table.
The bacterium include 1 chromosome and 2 plasmid sequences, NCBI registration number be respectively CP018763, CP018764、CP018765。
Pediococcus acidilactici BCC-1 achieved above was preserved in into China General Microbiological strain on November 4th, 2016 to protect (abbreviation CGMCC, address is at Tibetan center:Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postal Political affairs are encoded:100101), deposit number is CGMCC NO.13242, and Classification And Nomenclature is Pediococcus acidilactici (Pediococcus acidilactici)。
Embodiment 2
A kind of probiotics, its active component includes Pediococcus acidilactici (Pediococcus acidilactici) BCC- 1 and XOS (xylo-oligosaccharide);Wherein Pediococcus acidilactici BCC-1 content is 1 × 1010cfu/g;XOS mass fraction is 45%.
The present embodiment also provides the preparation method of the probiotics, comprises the following steps:
1) after the seed liquor that Pediococcus acidilactici BCC-1 is prepared with MRS culture mediums, liquid is carried out according to 1% inoculum concentration inoculation Body fermented and cultured.
2) culture medium that zymotic fluid is used is MRS culture medium, and fermentation condition is 30 DEG C, and rotating speed is 200rpm, is adjusted with ammoniacal liquor It is 6.5, anaerobic fermentation 24h to save pH, adds the sucrose solution that 200mL mass fractions are 30% after the 8h that ferments in every 3L nutrient solutions.
3) it is accurate to claim fermentation 1L streptococcus acidi lactici fermented solutions, 300g starch is added thereto, after spray drying, is obtained every gram of bacterium powder and is contained Some biodiasmin quantity is 2 × 1010cfu。
4) above-mentioned lactobacillus preparation and the xylo-oligosaccharide of 90% purity are according to 1:After the quality such as 1 is mixed, required answer is made Probiotics is closed, viable bacteria concentration is 1 × 1010cfu/g;Xylo-oligosaccharide content is 45%.
In broiler fodder, wheat content>When 30%, the content of above-mentioned probiotics is 150g/t in feed;Small Wheat content<When 30%, the content of above-mentioned probiotics is 100g/t in feed.
Experimental example 1
Pediococcus acidilactici BCC-1 used of the invention is separated from white meat-type chickens caecum chyme.When fermenting in vitro, enter Enter after stationary phase, the OD600 of bacterium solution is more than glucose (Glucose), FOS (FOS) and wood when BCC-1 is bred using XOS Sugared (Xylose), thus XOS has more preferable cultivation effect to BCC-1.Experimental result is shown in Fig. 2.As seen from Figure 2, lacking During weary carbon source, Pediococcus acidilactici can hardly grow (control group Control).FOS has very weak growth promoting function, carefully Bacteria growing shows that Pediococcus acidilactici is not strong to FOS Utilization ability to the death that falls into recession after 6h.Xylose is to Pediococcus acidilactici Preferable growth promoting function is shown, but its effect is slightly weaker than glucose.And XOS is better than wood to the growth promoting function of lactic acid bacteria Sugar, although the action effect of bacterial growth early stage is weaker than glucose, in bacterium stationary phase, with glucose identical imitate Really.
Experimental example 2 improves utilization rate of the broiler chicken to wheat
Experiment is randomly divided into 4 daily ration treatment groups from close 1 aa broiler chicken 480 of body weight, each processing 8 Repeat, each 15 chickens of weight.4 treatment groups are divided into control group and 3 test process groups.Experiment is using the configuration of full Wheat Based Diet (wheat content be 64.53%), during which broiler chicken free choice feeding and drinking-water, experiment breeding cycle is 1-21d.Test observation determines chicken group
Production performance, chyme stickiness and pH value, enteron aisle short-chain fat acid content.
The experiment of table 1 packet and processing
Table 2 adds influence of the Pediococcus acidilactici to broiler chicken 21d production performances
As can be seen from Table 2, (A groups), the addition of XOS and Pediococcus acidilactici compared with control group, can improve broiler chicken 21 The BWG (body weight gains) and FI (feed feed intake) in its age, while significantly reducing the feed-weight ratio (P of broiler chicken<0.05).And XOS and breast The compatibility of sour piece coccus reduces FCR (feed-weight ratio) using can further improve the BWG and FI of broiler chicken, shows best Production performance.
Table 3 adds influence of the Pediococcus acidilactici to broiler chicken 21d enteron aisles Chyme pH and viscosity
As can be seen from Table 3, addition XOS does not influence the chymeviscosity of 21d broiler chicken ileum pH value and jejunum, but can be notable Reduce the pH value (P of ileum<0.05).Addition Pediococcus acidilactici does not equally influence, but can significantly reduce blind on the pH of ileum The pH of the intestines and chymeviscosity (P of jejunum<0.05).XOS is used with the compatibility of Pediococcus acidilactici, compared with C groups, can be further Reduce the chymeviscosity of jejunum.
Table 4 adds influence of the Pediococcus acidilactici to broiler chicken 21d caecums lactic acid and volatile acid content
As can be seen from Table 4, the addition of XOS and Pediococcus acidilactici, can improve lactic acid content (P in 21d broiler chicken caecums >0.05);And XOS using cooperatively with Pediococcus acidilactici, but reduce the content (P of lactic acid in broiler chicken caecum>0.05).XOS is same The addition of lactic acid bacteria, is improved the trend of acetic acid content in chyme, and with the content highest (P=0.091) of D groups.With control group Compare, the addition of lactic acid bacteria is improved the trend of butyric acid content in chyme;XOS is used with Pediococcus acidilactici compatibility, can be notable Raising chyme in butyric acid content (P<0.05).XOS and the addition of Pediococcus acidilactici, it is not notable to acid content total in chyme Influence.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
SEQUENCE LISTING
<110>China Agricultural University
<120>Pediococcus acidilactici BCC-1 and its application of the one plant height effect using xylo-oligosaccharide
<130> KHP171110289.0
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1440
<212> DNA
<213>Pediococcus acidilactici(Pediococcusacidilactici)
<400> 1
agtcgaacga acttccgtta attgattatg acgtgcttgc actgaatgag attttaacac 60
gaagtgagtg gcggacgggt gagtaacacg tgggtaacct gcccagaagc aggggataac 120
acctggaaac agatgctaat accgtataac agagaaaacc gcctggtttt cttttaaaag 180
atggctctgc tatcacttct ggatggaccc gcggcgcatt agctagttgg tgaggtaacg 240
gctcaccaag gcgatgatgc gtagccgacc tgagagggta atcggccaca ttgggactga 300
gacacggccc agactcctac gggaggcagc agtagggaat cttccacaat ggacgcaagt 360
ctgatggagc aacgccgcgt gagtgaagaa gggtttcggc tcgtaaagct ctgttgttaa 420
agaagaacgt gggtgagagt aactgttcac ccagtgacgg tatttaacca gaaagccacg 480
gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttatc cggatttatt 540
gggcgtaaag cgagcgcagg cggtctttta agtctaatgt gaaagccttc ggctcaaccg 600
aagaagtgca ttggaaactg ggagacttga gtgcagaaga ggacagtgga actccatgtg 660
tagcggtgaa atgcgtagat atatggaaga acaccagtgg cgaaggcggc tgtctggtct 720
gtaactgacg ctgaggctcg aaagcatggg tagcgaacag gattagatac cctggtagtc 780
catgccgtaa acgatgatta ctaagtgttg gagggtttcc gcccttcagt gctgcagcta 840
acgcattaag taatccgcct ggggagtacg accgcaaggt tgaaactcaa aagaattgac 900
gggggcccgc acaagcggtg gagcatgtgg tttaattcga agctacgcga agaaccttac 960
caggtcttga catcttctgc caacctaaga gattaggcgt tcccttcggg gacagaatga 1020
caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg 1080
agcgcaaccc ttattactag ttgccagcat tcagttgggc actctagtga gactgccggt 1140
gacaaaccgg aggaaggtgg ggacgacgtc aaatcatcat gccccttatg acctgggcta 1200
cacacgtgct acaatggatg gtacaacgag tcgcgaaacc gcgaggttta gctaatctct 1260
taaaaccatt ctcagttcgg actgtaggct gcaactcgcc tacacgaagt cggaatcgct 1320
agtaatcgcg gatcagcatg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg 1380
tcacaccatg agagtttgta acacccaaag ccggtggggt aacctttagg agctagccgt 1440

Claims (10)

  1. Pediococcus acidilactici 1. (Pediococcus acidilactici) BCC-1, its deposit number is CGMCC NO.13242.
  2. 2. the cultural method of the Pediococcus acidilactici BCC-1 described in claim 1, it is characterised in that trained using MRS culture mediums Support.
  3. 3. Pediococcus acidilactici BCC-1 according to claim 2 cultural method, it is characterised in that including using MRS cultures Base carries out fermented and cultured, and fermentation condition is 30 DEG C, and rotating speed is 200rpm, is 6.5, anaerobic fermentation 24h with ammoniacal liquor regulation pH;Fermentation The sucrose solution that 200mL mass fractions are 30% is added after 8h in every 3L nutrient solutions.
  4. 4. the probiotics prepared as the Pediococcus acidilactici BCC-1 described in claim 1.
  5. 5. probiotics according to claim 4, it is characterised in that its active component includes Pediococcus acidilactici BCC-1 And XOS;
    Preferably, Pediococcus acidilactici BCC-1 content is 1-2 × 10 in the probiotics10cfu/g;XOS mass fraction For 45-47.5%.
  6. 6. probiotics according to claim 4, it is characterised in that Pediococcus acidilactici BCC- in the probiotics 1 content is 1 × 1010cfu/g;XOS mass fraction is 45%.
  7. 7. the preparation method of probiotics described in claim 6, it is characterised in that comprise the following steps:
    1) after the seed liquor that Pediococcus acidilactici BCC-1 is prepared with MRS culture mediums, liquid hair is carried out according to 1% inoculum concentration inoculation Ferment culture;
    2) culture medium that zymotic fluid is used is MRS culture medium, and fermentation condition is 30 DEG C, and rotating speed is 200rpm, and pH is adjusted with ammoniacal liquor For 6.5, anaerobic fermentation 24h;The sucrose solution that 200mL mass fractions are 30% is added after fermentation 8h in every 3L nutrient solutions;
    3) it is accurate to claim fermentation 1L streptococcus acidi lactici fermented solutions, 300g starch is added thereto, after spray drying, obtains what every gram of bacterium powder contained Biodiasmin quantity is 2 × 1010cfu;
    4) by the xylo-oligosaccharide of gained lactic acid bacteria bacterium powder and 90% purity according to 1:After the quality such as 1 is mixed, it is made required and is combined Probiotics, viable bacteria concentration is 1 × 1010cfu/g;Xylo-oligosaccharide content is 45%.
  8. 8. any one of Pediococcus acidilactici BCC-1 or claim 4-6 described in claim 1 probiotics is dynamic in preparation Application in thing feed;Preferably, the animal feed is the daily ration prepared using raw material based on wheat;Preferably, it is described Animal includes broiler chicken, laying hen, meat duck, egg duck, live pig.
  9. 9. it is dynamic containing any one of the Pediococcus acidilactici BCC-1 or claim 4-6 described in claim 1 probiotics Thing feed.
  10. 10. animal feed according to claim 9, it is characterised in that the animal feed is to use original based on wheat Expect the daily ration prepared;When wheat weighs less than 30% in complete feed, the addition of the probiotics is 0.01%, or Person controls the content 1 × 10 of Pediococcus acidilactici BCC-1 in the complete feed9Cfu/kg, XOS mass fraction are 0.005%; When wheat weight is more than 30% in complete feed, the addition of the probiotics is 0.015%, or is controlled described complete Pediococcus acidilactici BCC-1 content is 1.5 × 10 in valency feed10Cfu/g, XOS mass fraction are 0.075%.
CN201710074474.4A 2017-02-10 2017-02-10 Pediococcus acidilactici BCC-1 capable of efficiently utilizing xylo-oligosaccharide and application thereof Active CN107058170B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710074474.4A CN107058170B (en) 2017-02-10 2017-02-10 Pediococcus acidilactici BCC-1 capable of efficiently utilizing xylo-oligosaccharide and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710074474.4A CN107058170B (en) 2017-02-10 2017-02-10 Pediococcus acidilactici BCC-1 capable of efficiently utilizing xylo-oligosaccharide and application thereof

Publications (2)

Publication Number Publication Date
CN107058170A true CN107058170A (en) 2017-08-18
CN107058170B CN107058170B (en) 2020-11-24

Family

ID=59598795

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710074474.4A Active CN107058170B (en) 2017-02-10 2017-02-10 Pediococcus acidilactici BCC-1 capable of efficiently utilizing xylo-oligosaccharide and application thereof

Country Status (1)

Country Link
CN (1) CN107058170B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182184A (en) * 2018-09-17 2019-01-11 吉林农业大学 One plant of dog source Pediococcus acidilactici and its application
CN110463823A (en) * 2018-12-17 2019-11-19 河南中微生物科技研究院(有限合伙) A kind of micro-ecological additive and preparation method thereof improving animal intestinal tract health
CN113100355A (en) * 2021-05-19 2021-07-13 华南农业大学 Feed additive for improving meat quality of tilapia and feed
CN113388546A (en) * 2021-06-21 2021-09-14 齐鲁工业大学 Composite microecological preparation and application thereof in improving intestinal short-chain fatty acids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101054551A (en) * 2007-04-10 2007-10-17 天津生机集团有限公司 Probiotics composition capable of accelerating growth of beef cattle
CN103392911A (en) * 2013-08-06 2013-11-20 国家粮食局科学研究院 Feed-use high-activity lactobacillus solid preparation and preparation method thereof
WO2016100881A1 (en) * 2014-12-18 2016-06-23 Microbios, Inc. Prebiotic and bacterial-based probiotic pathogen inhibitor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101054551A (en) * 2007-04-10 2007-10-17 天津生机集团有限公司 Probiotics composition capable of accelerating growth of beef cattle
CN103392911A (en) * 2013-08-06 2013-11-20 国家粮食局科学研究院 Feed-use high-activity lactobacillus solid preparation and preparation method thereof
WO2016100881A1 (en) * 2014-12-18 2016-06-23 Microbios, Inc. Prebiotic and bacterial-based probiotic pathogen inhibitor

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FALCK P ET AL.: "Xylooligosaccharides from hardwood and cereal xylans produced by a thermostable xylanase as carbon sources for Lactobacillus brevis and Bifidobacterium adolescentis.", 《J AGRIC FOOD CHEM.》 *
MICHLMAYR H ET AL.: "Arabinoxylan oligosaccharide hydrolysis by family 43 and 51 glycosidases from Lactobacillus brevis DSM 20054.", 《APPL ENVIRON MICROBIOL.》 *
SEYED HOSSEIN HOSEINIFA ET AL: "Determination of the best symbiotic between probiotic bacteria Pediococcus acidilactici and prebiotics inulin, oligofructose and xylooligosaccharide", 《BIOLGICAL JOURNAL OF MICROORGANISM》 *
王津 等: "低聚糖对乳酸片球菌体外增殖和抑菌效果的影响", 《中国饲料》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182184A (en) * 2018-09-17 2019-01-11 吉林农业大学 One plant of dog source Pediococcus acidilactici and its application
CN109182184B (en) * 2018-09-17 2021-07-20 吉林农业大学 Pediococcus acidilactici strain and application thereof
CN110463823A (en) * 2018-12-17 2019-11-19 河南中微生物科技研究院(有限合伙) A kind of micro-ecological additive and preparation method thereof improving animal intestinal tract health
CN113100355A (en) * 2021-05-19 2021-07-13 华南农业大学 Feed additive for improving meat quality of tilapia and feed
CN113388546A (en) * 2021-06-21 2021-09-14 齐鲁工业大学 Composite microecological preparation and application thereof in improving intestinal short-chain fatty acids

Also Published As

Publication number Publication date
CN107058170B (en) 2020-11-24

Similar Documents

Publication Publication Date Title
CN103820363B (en) A kind of preparation and application of faecium bacterium powder
CN106148249B (en) It is a kind of suitable for the lactic acid bacteria agent of grass silage and its application
CN102356816B (en) Animal health care probiotics leavening capable of improving meat quality and application thereof
CN107058170A (en) Pediococcus acidilactici BCC 1 and its application of the one plant height effect using xylo-oligosaccharide
CN1813562A (en) Compound microbial fermented fodder nutrient additive and its preparing method
CN104293696A (en) Enterococcus faecalis HEW-A131 and application thereof
CN102805209A (en) Synbiotics feed additive for beasts and birds and application thereof
CN103468594B (en) Candidautilis strain and application thereof
CN103045498B (en) Bacillus amyloliquefaciens and application thereof
CN1903058A (en) Ternary active microbiological prepns. for animals and birds
CN103184174B (en) Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium
CN104206645A (en) Method for producing small peptide feed additive by solid fermentation of soybean meal with aspergillus oryzae
CN106854627A (en) A kind of composite bacteria agent for promoting the flat angle stratiomyiid larval growth of speck and application
CN107974421A (en) A kind of lactobacillus acidophilus and its screening technique and application, a kind of microbial inoculum
CN102936568B (en) Solid fermentation inoculum of chicken-origin bacillus subtilis and pediococcus pentosaceus and preparation method and application of solid fermentation inoculum
CN111690574B (en) Weissella mesenteroides and application thereof
CN106906166A (en) One boar source Lactobacillus salivarius active bacteria formulation and application
CN104263683B (en) Bacillus pumilus 315 and its application with prebiotic effect
CN105132321A (en) Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method
CN110257292A (en) A kind of microbial bacterial agent used for aquiculture and preparation method thereof
CN102093966B (en) Mink-derived Lactobacillus plantarum strain MDL1118 and application thereof
CN103266074A (en) B.subtilis spores strain and application thereof
CN112961806A (en) Bacillus coagulans capable of producing lactic acid at high yield, biological fermentation feed and preparation method and application thereof
CN1687400A (en) Sporolactobacillaceae and produced preparation of living fungus thereof
CN108719624A (en) A kind of compound microecological fermentation preparation and production method improving beef cattle meat

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant