CN101444252B - Method for preparing fermented virus-free cottonseed meal feed protein - Google Patents

Method for preparing fermented virus-free cottonseed meal feed protein Download PDF

Info

Publication number
CN101444252B
CN101444252B CN2008102191972A CN200810219197A CN101444252B CN 101444252 B CN101444252 B CN 101444252B CN 2008102191972 A CN2008102191972 A CN 2008102191972A CN 200810219197 A CN200810219197 A CN 200810219197A CN 101444252 B CN101444252 B CN 101444252B
Authority
CN
China
Prior art keywords
cottonseed meal
rice
strain
cottonseed
fermented
Prior art date
Application number
CN2008102191972A
Other languages
Chinese (zh)
Other versions
CN101444252A (en
Inventor
叶明强
邝哲师
徐志宏
赵祥杰
罗国庆
肖更生
潘木水
陈薇
Original Assignee
广东省农业科学院农业生物技术研究所
广东宝桑园健康食品研究发展中心
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 广东省农业科学院农业生物技术研究所, 广东宝桑园健康食品研究发展中心 filed Critical 广东省农业科学院农业生物技术研究所
Priority to CN2008102191972A priority Critical patent/CN101444252B/en
Publication of CN101444252A publication Critical patent/CN101444252A/en
Application granted granted Critical
Publication of CN101444252B publication Critical patent/CN101444252B/en

Links

Abstract

The present invention discloses a method for preparing fermented virus-free cottonseed meal feed protein. The invention relates to a production process which uses cottonseed meal as raw material and produces the virus-free cottonseed meal feed protein by microbial technology in a transformation way, in particular to a microbial fermenting process of using multi-strains mixed solid to ferment and prepare virus-free cottonseed meal. Fermentation cultures of one strain Saccharomyces cerevisiae which is cultured in an amplification way, one strain Candida tropicalis, one strain Bacillus subtilis and one strain Lactobacillus are evenly mixed in the proportion of 2 to 1 to 1 to 1 and are inoculated in cottonseed meal (through 40 mesh sieve) with 5 percent of the inoculation amount, wherein, the water content of the cottonseed meal is 50 percent; simultaneously, substrate culture media (5 percent of molasses, 5 percent of bran, 0.5 percent of ammonium sulfate, and the initial pH value is 6.0) are added, evenly mixed, fermented for 72hours at 30 DEG C in aerobic way, dried and sampled at 60 DEG C; finally, the nutrient analysis is carried out on the cottonseed meal before and after fermentation; when weighed and packaged, finished products of the fermented cottonseed meal can be stored for 6 months at a cool and dry place. The fermented cottonseed meal can be used as feeds for beef cattle, chicken, tilapia, etc.

Description

A kind of preparation method of fermented virus-free cottonseed meal feed protein
Technical field
The present invention relates to the cottonseed dregs of rice is raw material, utilizes microbial technique to transform a kind of production technology of producing virus-free cottonseed meal feed protein, relates in particular to the microorganism fermentation process that a kind of many solid type mixed culture fermentations prepare virus-free cottonseed meal.
Background technology
In recent years, China's animal husbandry and feed industrial development are very rapid, and the contradiction that feed resource is in short supply becomes increasingly conspicuous.According to scholarly forecast, 2010~the year two thousand twenty, the difference of China's protein feeds is 2400~4,800 ten thousand tons, puffed soybean difference is 2,560 ten thousand tons.For a long time, the protein feed raw material dregs of beans that China mainly relies on, because of being subjected to influence of various factors such as output, weather, domestic and international market price fluctuation, supply day is becoming tight, and price irregularly goes up and fluctuates, and is bigger to the Feed Enterprise cost impact.Though and more existing its quantity of agricultural byproducts is abundant and price is cheap, because of containing ANFs or being subject to conventional machining process, has limited its effective utilization.At these problems, relevant researcher has carried out the developmental research of a lot of aspects about protein feeds both at home and abroad, and wherein effective utilization of the relevant cottonseed dregs of rice is exactly one of research emphasis.
The cottonseed dregs of rice are China's another kind of important puffed soybean protein feeds except that dregs of beans (cake), but because of containing to the virulent free gossypol of animal and some ANFs, its value and use amount are subjected to very big restriction, in order to enlarge its range of application, must carry out detoxification treatment earlier and be worth to improve applied nutrition.The main poison-removing method of the cottonseed dregs of rice has physics method, chemical method and bioanalysis at present.Though physics method and chemical detoxication method can significantly reduce free gossypol content in the cotton cake dregs, all have certain shortcoming.Handle the feeding value that the residual material in back and color and luster problem have often reduced cotton cake dregs with chemicals, organic solvent.Bioanalysis comprises enzymatic isolation method and microbe fermentation method, and the enzymatic isolation method detoxification efficiency is undesirable.Microbe fermentation method is a kind of new cotton cake dregs detoxification approach that at first grows up in China in the later stage eighties 20th century.This kind method not only detoxification efficiency is good, avoids the influence to the cottonseed protein functional character of chemical addition agent and physics method, also can improve palatability, increases protein content; The various digestive ferments that microbial fermentation produces, amino acid, vitamin etc. have also improved the nutritive value of cotton cake dregs greatly.Therefore, the cottonseed dregs of rice after microbial fermentation is handled, not only can be used as ruminant and aquatic animal feed, but also can partly replace protein feeds such as dregs of beans, fish meal in the livestock and poultry diet, or be used with other meal expeller and mealsolvent, both safe in utilization, can reduce cost again, increase economic efficiency, have good development potentiality.
The technology of the present invention is primarily aimed at the key issue of above-mentioned cottonseed dregs of rice detoxification, fermentation parameter by the many solid type mixed culture fermentations of system research, finally obtained a kind of microbial fermentation processes for preparing virus-free cottonseed meal feed protein, this all has important theory and realistic meaning for improving cottonseed dregs of rice microbial fermentation virus elimination rate and the aspects such as effective utilization of cottonseed cake protein in animal husbandry.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of fermented virus-free cottonseed meal feed protein, further promote the effective utilization of cotton cake dregs in animal husbandry, make it become one of approach that solves the present protein feed resource shortage of China.
Purpose of the present invention is achieved by the following technical programs:
The preparation method of a kind of fermented virus-free cottonseed meal feed protein provided by the invention the steps include:
A. belong to picking one ring thalline bacterium and the Blastocystis fungi from original bacterial classification hay bacillus, be inoculated into respectively in LB seed culture medium and the brewer's wort seed culture medium, under 30 ℃ of-37 ℃ of temperature, 150-170rpm, shaking table is cultivated after 16-24 hour, up to OD 600=0.7-0.9 stops to cultivate, as seed liquor.Picking one ring thalline is inoculated in the genus lactubacillus culture medium from original bacterial classification genus lactubacillus bacterium, and static cultivation is 40-48 hour under 30 ℃ of-35 ℃ of temperature, as seed liquor.
B. in liquid enlarging LB culture medium (hay bacillus belongs to bacterium) and malt extract medium (Blastocystis fungi), inoculate seed liquor with the 1-5% inoculum concentration, under 30 ℃ of-37 ℃ of temperature, 150-170rpm, shaking table was cultivated 20-28 hour.With 1-5% inoculum concentration inoculation seed liquor, static cultivation is 48 hours under 30 ℃ of-35 ℃ of temperature in liquid expansion genus lactubacillus culture medium (genus lactubacillus bacterium).The various strain fermentation nutrient solutions of saccharomyces cerevisiae, candida tropicalis, bacillus subtilis, lactic acid bacteria are mixed in the 2:1:1:1 ratio, as cottonseed dregs of rice fermented bacterium liquid.
C. cottonseed dregs of rice fermented bacterium liquid among the b being inoculated into water content with 5% inoculum concentration is in 50% the cottonseed dregs of rice (crossing 40 mesh sieves), add substrate cultivation base (molasses 5% simultaneously, wheat bran 5%, ammonium sulfate 0.5%, initial pH value is 6.0) and mix, aerobic fermentation is 72 hours under 30 ℃ of temperature, then in 60 ℃ of dry sample preparations down, at last the cottonseed dregs of rice before and after the fermentation is carried out the nutritional labeling analysis.
D. the cottonseed dregs of rice finished product that ferments is put in shady and cool dry place after weighing, packing, at room temperature can deposit 6 months.
E. the cottonseed dregs of rice after the fermentation promptly can be used as the feed of beef cattle, chicken, Tilapia mossambica etc.
Bacterial classification seed LB culture medium described in the step a of the present invention contains the sodium chloride of 0.3%-0.5%, peptone and the 0.5%-1.0% yeast extract of 0.3%-0.5% by weight percentage, and the genus lactubacillus culture medium contains the glucose, 0.3% KH of NaCl, 0.2%-0.4% of tryptone, the 0.3%-0.5% of 0.2%-0.4% 2PO 4, 0.3% K 2HPO 4, 0.02% MgCl 2, 0.005% CaSO 4With 0.2% starch, malt extract medium contains 10% malt extract powder and 0.01% chloramphenicol.
Bacterial classification described in the step a of the present invention is bacillus subtilis (Bacillus subtilis) 1 strain, lactic acid bacteria (Lactobacillus) 1 strain, saccharomyces cerevisiae (Saccharomyces crevisiae) 1 strain and candida tropicalis (Candida tropicalis) 1 strain.
The prescription of three kinds of fluid enlargement culture bases described in the step b of the present invention is identical with step a.
The present invention has following beneficial effect: the present invention adopts the microbial fermentation processing cottonseed dregs of rice, and not only detoxification efficiency is good, has avoided the influence to the cottonseed protein functional character of chemical addition agent and physics method, also can improve palatability, increases protein content; The various digestive ferments that sweat produces, amino acid, vitamin etc. have also improved the nutritive value of the cottonseed dregs of rice greatly.Therefore, the cotton cake dregs after microbial fermentation is handled is even also can partly replace protein feeds such as dregs of beans, fish meal in livestock and poultry diet, or be used with other meal expeller and mealsolvent, both safe in utilization, can reduce cost again, increase economic efficiency, have good development potentiality.
The specific embodiment
The preparation method's of a kind of fermented virus-free cottonseed meal feed protein provided by the invention embodiment is as follows:
The used bacterial classification of present embodiment is bacillus subtilis (Bacillus subtills) 1 strain, lactic acid bacteria (Lactobacillus) 1 strain, saccharomyces cerevisiae (Saccharomyces crevisiae) 1 strain and candida tropicalis (Candida tropicalis) 1 strain.Its concrete steps are:
A. the LB fluid nutrient medium of being made up of 5 gram peptones, 5 gram yeast extracts, 3 gram sodium chloride and 1 premium on currency (pH7.0) is poured in the aseptic triangular flask, makes bacillus subtilis seed fluid nutrient medium and fluid enlargement culture base; By 2 gram peptones, 5 gram NaCl, 2 gram glucose, 3 gram KH 2PO 4, 3 the gram K 2HPO 4, 0.2 the gram MgCl 2, 0.05 the gram CaSO 4, the genus lactubacillus culture medium formed of 2 gram starch and 1 premium on currency (pH6.5),, pour in the aseptic triangular flask, make lactobacillus solution body culture medium and fluid enlargement culture base; By the malt extract medium that 100 gram malt extract powder, 0.01 gram chloramphenicol and 1 premium on currency (pH6.0) are formed, pour in the aseptic triangular flask, make saccharomycete seed fluid nutrient medium and fluid enlargement culture base.
B. belong to picking one ring thalline bacterium and the Blastocystis fungi from original bacterial classification hay bacillus, be inoculated into respectively in LB seed culture medium and the brewer's wort seed culture medium, under 30 ℃ of-37 ℃ of temperature, 150-170rpm, shaking table is cultivated after 16-24 hour, up to OD 600=0.7-0.9 stops to cultivate, as seed liquor.Picking one ring thalline is inoculated in the genus lactubacillus culture medium from original bacterial classification genus lactubacillus bacterium, and static cultivation is 40-48 hour under 30 ℃ of-35 ℃ of temperature, as seed liquor.
C. in liquid enlarging LB culture medium (hay bacillus belongs to bacterium) and malt extract medium (Blastocystis fungi), inoculate seed liquor with the 1-5% inoculum concentration, under 30 ℃ of-37 ℃ of temperature, 150-170rpm, shaking table was cultivated 20-28 hour.With 1-5% inoculum concentration inoculation seed liquor, static cultivation is 48 hours under 30 ℃ of-35 ℃ of temperature in liquid expansion genus lactubacillus culture medium (genus lactubacillus bacterium).The strain fermentation nutrient solution of saccharomyces cerevisiae, candida tropicalis, bacillus subtilis, lactic acid bacteria is mixed in the 2:1:1:1 ratio, as cottonseed dregs of rice fermented bacterium liquid.
D. cottonseed dregs of rice fermented bacterium liquid being inoculated into water content with 5% inoculum concentration is in 50% the cottonseed dregs of rice (crossing 40 mesh sieves), add substrate cultivation base (molasses 5% simultaneously, wheat bran 5%, ammonium sulfate 0.5%, initial pH value is 6.0) and mix, aerobic fermentation is 72 hours under 30 ℃ of temperature, then in 60 ℃ of dry sample preparations down.
E. the cottonseed dregs of rice before and after the fermentation are carried out the nutritional labeling analysis.
F. the cottonseed dregs of rice finished product that ferments is put in shady and cool dry place after weighing, packing, at room temperature can deposit 6 months.
G. the cottonseed dregs of rice after fermenting among the step f promptly can be used as the feed of beef cattle, chicken, Tilapia mossambica etc.
The ANFs of the cottonseed dregs of rice finished product of microbe fermentation method preparation obviously reduces in above-mentioned example, crude protein and soluble protein content are increased, middle small molecular weight protein showed increased, improved palatability greatly, amino acid content tends to balance, vitamin is abundant, and contains beneficial bacterium and UGF, and nutritive value improves greatly.Can improve use amount and the scope of application of the cottonseed dregs of rice on animal produces more significantly.Its effect is as follows:
Select yellow fryer 480 plumages in the healthy 21 active age in days commodity south of the Five Ridges, adopt the single factor experiment design, establish 4 groups, establish 4 repetitions for every group, each repeats 30 plumages.Control group is for adding the typical daily ration of the 3% common cottonseed dregs of rice, and the common cottonseed dregs of rice and part dregs of beans that test group 1,2,3 is added respectively in the alternative basal diet of 5%, the 8% and 10% fermentation cottonseed dregs of rice are formulated.26 ages in days enter formal test, 22 days experimental periods.The growth performance index of last determination test fryer, butcher performance, alimentary canal ponderal index, immune indexes, blood parameters.The result is:
(1) adds 5%, 8% and 10% the fermentation cottonseed dregs of rice in the daily ration of broiler respectively to all not remarkable (p〉0.05) (table 1) of average daily ingestion amount, average daily gain and the feed conversion rate difference of fryer; (2) add 5%, 8% and 10% the fermentation cottonseed dregs of rice in the daily ration of broiler respectively to complete clean thorax rate, half clean thorax rate, chest muscle rate and the leg flesh rate difference of fryer all not significantly (p〉0.05), meat production is good.Add 10% the fermentation cottonseed dregs of rice in the daily ration, dressing percentage is significantly higher than and adds 5% the fermentation cottonseed dregs of rice, and along with the rising of adding fermentation cottonseed dregs of rice ratio in the daily ration, dressing percentage and half clean thorax rate have trend of rising (table 2); (3) add in the daily ration 5% the fermentation cottonseed dregs of rice extremely significantly (p<0.01) reduced the abdomen fat rate of fryer, but along with the rising of fermentation cottonseed dregs of rice ratio in the daily ration, fryer abdomen fat rate has trend of rising (table 2); (4) add 5%, 8% and 10% the fermentation cottonseed dregs of rice in the daily ration of broiler respectively alimentary canal, immune organ and the blood parameters aspect of fryer are not all had negative effect (table 3-5); (5) the cottonseed dregs of rice that adopt this experimental condition and mode to ferment, adding proportion can reach 10% in daily ration of broiler, but best with the fermentation cottonseed dregs of rice feeding effect that adds 5% ratio.
Table 1 adds the fermentation cottonseed dregs of rice of different proportion to the growth of meat chicken Effect on Performance
Annotate: the different differences extremely significantly (p<0.01) of representing of colleague's shoulder motes capitalization, the different significant differences (p<0.05) of representing of colleague's shoulder motes lowercase, colleague's shoulder motes identical lowercase represent difference not significantly (p〉0.05), and the mark person does not represent difference not remarkable, down with.
The fermentation cottonseed dregs of rice that add different proportion in table 2 daily ration are butchered the influence of index to fryer
Table 3 adds the influence of the fermentation cottonseed dregs of rice of different proportion to fryer alimentary canal ponderal index
Table 4 adds the influence of the fermentation cottonseed dregs of rice of different proportion to the fryer immune organ
Table 5 adds the influence of the fermentation cottonseed dregs of rice of different proportion to the fryer blood parameters

Claims (3)

1. the preparation method of a fermented virus-free cottonseed meal feed protein the steps include:
A. belong to picking one ring thalline bacterium and the Blastocystis fungi from original bacterial classification hay bacillus, be inoculated into respectively in LB seed culture medium and the brewer's wort seed culture medium, under 30 ℃ of-37 ℃ of temperature, 150-170rpm, shaking table is cultivated after 16-24 hour, up to OD 600=0.7-0.9 stops to cultivate, and as seed liquor, picking one ring thalline is inoculated in the genus lactubacillus culture medium from original bacterial classification genus lactubacillus bacterium, and static cultivation is 40-48 hour under 30 ℃ of-35 ℃ of temperature, as seed liquor;
B. in liquid enlarging LB culture medium and malt extract medium, inoculate hay bacillus respectively and belong to bacterium and Blastocystis fungi seed liquor with the 1-5% inoculum concentration, under 30 ℃ of-37 ℃ of temperature, 150-170rpm, shaking table was cultivated 20-28 hour, enlarge in the genus lactubacillus culture medium with 1-5% inoculum concentration inoculating lactic acid Pseudomonas bacterium seed liquor at liquid, static cultivation is 48 hours under 30 ℃ of-35 ℃ of temperature, with saccharomyces cerevisiae, candida tropicalis, bacillus subtilis, the various strain fermentation nutrient solutions of lactic acid bacteria were in 2: 1: 1: 1 ratio mixes, as cottonseed dregs of rice fermented bacterium liquid;
C. cottonseed dregs of rice fermented bacterium liquid among the b being inoculated into water content with 5% inoculum concentration is in 50% the cottonseed dregs of rice, add the substrate cultivation base simultaneously: molasses 5%, wheat bran 5%, ammonium sulfate 0.5%, initial pH value is 6.0, and mixes, and aerobic fermentation is 72 hours under 30 ℃ of temperature, in 60 ℃ of dry sample preparations down, at last the cottonseed dregs of rice before and after the fermentation are carried out the nutritional labeling analysis then;
D. the cottonseed dregs of rice finished product that ferments is put in shady and cool dry place after weighing, packing, at room temperature can deposit 6 months;
E. the cottonseed dregs of rice after the fermentation are as the feed of beef cattle, pig, chicken, Tilapia mossambica.
2. the preparation method of fermented virus-free cottonseed meal feed protein according to claim 1, LB seed culture medium described in its step a contains the sodium chloride of 0.3%-0.5%, peptone and the 0.5%-1.0% yeast extract of 0.3%-0.5% by weight percentage, and the genus lactubacillus culture medium contains the glucose, 0.3% KH of NaCl, 0.2%-0.4% of tryptone, the 0.3%-0.5% of 0.2%-0.4% 2PO 4, 0.3% K 2HPO 4, 0.02% MgCl 2, 0.005% CaSO 4With 0.2% starch, malt extract medium contains 10% malt extract powder and 0.01% chloramphenicol.
3. the preparation method of fermented virus-free cottonseed meal feed protein according to claim 1, the bacterial classification described in its step a is bacillus subtilis (Bacillus subtilis) 1 strain, lactic acid bacteria (Lactobacillus) 1 strain, saccharomyces cerevisiae (Saccharomyces crevisiae) 1 strain and candida tropicalis (Candida tropicalis) 1 strain.
CN2008102191972A 2008-11-18 2008-11-18 Method for preparing fermented virus-free cottonseed meal feed protein CN101444252B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2008102191972A CN101444252B (en) 2008-11-18 2008-11-18 Method for preparing fermented virus-free cottonseed meal feed protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2008102191972A CN101444252B (en) 2008-11-18 2008-11-18 Method for preparing fermented virus-free cottonseed meal feed protein

Publications (2)

Publication Number Publication Date
CN101444252A CN101444252A (en) 2009-06-03
CN101444252B true CN101444252B (en) 2011-06-08

Family

ID=40740364

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008102191972A CN101444252B (en) 2008-11-18 2008-11-18 Method for preparing fermented virus-free cottonseed meal feed protein

Country Status (1)

Country Link
CN (1) CN101444252B (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101692868B (en) * 2009-10-20 2012-11-21 大连工业大学 Method for producing animal feed based on cottonseed meal physical, chemical and microbial detoxification technology
CN101810244B (en) * 2010-04-29 2011-02-16 湖南农业大学 Biological detoxification method of cottonseed meal
CN102352314B (en) * 2011-08-03 2012-07-11 湖南农业大学 Composite bacillus cottonseed meal fermenting agent and preparation method thereof
CN102293351B (en) * 2011-09-23 2013-01-23 中国水产科学研究院淡水渔业研究中心 Biological feed for cultivating spotted silver carps and feeding method thereof
CN102488087A (en) * 2011-12-26 2012-06-13 林元山 Biological detoxification method for camellia seed cakes
CN102965301B (en) * 2012-09-24 2015-03-25 上海交通大学 Lactobacillus crustorum strain and its application
CN102972628B (en) * 2012-11-29 2014-05-21 新疆希普生物科技股份有限公司 Preparation method of fermented cottonseed meal rich in biotin
CN103355472A (en) * 2013-01-21 2013-10-23 上海创博生态工程有限公司 Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof
CN103082083B (en) * 2013-02-21 2014-02-05 河北大学 Method for producing protein feed by virtue of microbial fermentation
CN103238757B (en) * 2013-05-30 2014-07-09 山东新希望六和集团有限公司 Compound feed containing fermented cottonseed meal for chicken for eggs and preparation method thereof
CN105410432A (en) * 2015-11-30 2016-03-23 安徽金丰源畜牧科技有限公司 Attractant layer feed for reducing egg cholesterol contents
CN105410436A (en) * 2015-11-30 2016-03-23 安徽金丰源畜牧科技有限公司 Egg cholesterol content lowering nutritional laying hen feed
CN105410435A (en) * 2015-11-30 2016-03-23 安徽金丰源畜牧科技有限公司 Egg cholesterol content lowering stomach strengthening laying hen feed
CN105410437A (en) * 2015-11-30 2016-03-23 安徽金丰源畜牧科技有限公司 Egg cholesterol content lowering health-care laying hen feed
CN105410433A (en) * 2015-11-30 2016-03-23 安徽金丰源畜牧科技有限公司 Environmentally friendly layer feed for reducing the cholesterol content of eggs
CN107319566A (en) * 2017-07-26 2017-11-07 广东省农业科学院蚕业与农产品加工研究所 A kind of sorosis litchi powder rich in probiotics and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1083671A (en) * 1993-09-13 1994-03-16 山东省聊城地区粮食局 De-poisoning method for cottonseed cake
CN1259299A (en) * 1999-11-24 2000-07-12 吉林大学 Dehusk and detoxin of cotton-seed cake to produce protein feed
US20030049363A1 (en) * 2001-01-25 2003-03-13 Ajinomoto U.S.A., Inc. Flowable cottonseed and method for its preparation
CN1802987A (en) * 2006-01-17 2006-07-19 广东省农业科学院农业生物技术研究所 Preparation method for transforming pineapple bran to biological feed stuff using microbe
CN101171953A (en) * 2006-11-03 2008-05-07 广东省农业科学院农业生物技术研究所 Process for producing agricultural wastes converted biological feedstuff
CN101297677A (en) * 2008-06-16 2008-11-05 南昌大学 Technique for detoxicating rapeseed dregs and modifying rapeseed proteins by synchronous bacterium mixing and fermenting method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1083671A (en) * 1993-09-13 1994-03-16 山东省聊城地区粮食局 De-poisoning method for cottonseed cake
CN1259299A (en) * 1999-11-24 2000-07-12 吉林大学 Dehusk and detoxin of cotton-seed cake to produce protein feed
US20030049363A1 (en) * 2001-01-25 2003-03-13 Ajinomoto U.S.A., Inc. Flowable cottonseed and method for its preparation
CN1802987A (en) * 2006-01-17 2006-07-19 广东省农业科学院农业生物技术研究所 Preparation method for transforming pineapple bran to biological feed stuff using microbe
CN101171953A (en) * 2006-11-03 2008-05-07 广东省农业科学院农业生物技术研究所 Process for producing agricultural wastes converted biological feedstuff
CN101297677A (en) * 2008-06-16 2008-11-05 南昌大学 Technique for detoxicating rapeseed dregs and modifying rapeseed proteins by synchronous bacterium mixing and fermenting method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
邝哲师等.豆粕抗营养因子的微生物发酵降解试验.《中国饲料》.2007,(第22期),27-28,43. *

Also Published As

Publication number Publication date
CN101444252A (en) 2009-06-03

Similar Documents

Publication Publication Date Title
CN103766650B (en) A kind of feed addictive of layer chicken and preparation thereof
CN104171538B (en) A kind of grain-saving type laying hen in egg laying peak period daily ration and preparation method thereof
CN101558823B (en) Fermented liquid feed for Pig and poultry as well as preparation method and application thereof
CN101107970B (en) Bion cow concentrated feed and method for making same
CN103082136B (en) Pig feed additive
CN101940267B (en) Method for preparing microbial fermentation feed for improving pork quality
CN101455274B (en) Method for producing feedstuff containing ganoderma lucidum active ingredient and feedstuff using ganoderma lucidum liquid fermentation liquid
CN101422210B (en) Feedstuff additive prepared by mixed fermentation of plant feedstuff protein and chitin processing-waste and processing method thereof
CN101297677B (en) Technique for detoxicating rapeseed dregs and modifying rapeseed proteins by synchronous bacterium mixing and fermenting method
CN101263862B (en) Food and rink castoff feed processing method and culture medium and confecting method thereby
CN103262963B (en) Live pig fattening feed taking fermented agricultural and sideline products as raw materials
CN101711548B (en) Fermented feed containing sodium humate and process and application thereof
CN101278702B (en) Novel microbial feed additive and method of producing the same
CN102232475B (en) Physiological regulator for male animals and preparation method thereof
CN101099543B (en) Aquatic biological feed and preparation method thereof
CN102599351B (en) Bio-enzyme and microorganism containing compound feed additive and preparation process thereof
CN101889628B (en) Process for producing biosynthesis feed
CN103749967B (en) Sweet potato dregs fermented feed and preparation method thereof
CN101341927A (en) Microbial feed additive liquid agent for pig and preparation technique
CN102318737B (en) Preparation method of nontoxic cottonseed meal animal feed
CN1207873A (en) Straw feed fermenting process
CN105614023B (en) Fermentation enzymolysis agent for soybean meal fermentation and application thereof
CN104054966B (en) Pig is fattened with biological fodder and its preparation method in a kind of Wuzhi Mountain
CN104472879A (en) Tea residue feed additive and preparation method thereof
CN102987140A (en) Method for preparing novel ruminant feed by using beneficial bacteria and plant fiber

Legal Events

Date Code Title Description
PB01 Publication
C06 Publication
SE01 Entry into force of request for substantive examination
C10 Entry into substantive examination
GR01 Patent grant
C14 Grant of patent or utility model
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20110608

Termination date: 20151118

EXPY Termination of patent right or utility model