CN105724550A - Method for performing bone and meat separation on fish wastes by utilizing microbial fermentation - Google Patents
Method for performing bone and meat separation on fish wastes by utilizing microbial fermentation Download PDFInfo
- Publication number
- CN105724550A CN105724550A CN201610129692.9A CN201610129692A CN105724550A CN 105724550 A CN105724550 A CN 105724550A CN 201610129692 A CN201610129692 A CN 201610129692A CN 105724550 A CN105724550 A CN 105724550A
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- Prior art keywords
- fish
- bacillus licheniformis
- fermentation
- separation
- pieces
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 47
- 238000000855 fermentation Methods 0.000 title claims abstract description 35
- 230000004151 fermentation Effects 0.000 title claims abstract description 35
- 238000000926 separation method Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 21
- 210000000988 bone and bone Anatomy 0.000 title abstract 4
- 235000013372 meat Nutrition 0.000 title abstract 4
- 230000000813 microbial effect Effects 0.000 title abstract 2
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 102000035195 Peptidases Human genes 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 5
- 235000021190 leftovers Nutrition 0.000 claims description 17
- 239000000126 substance Substances 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 4
- 235000019833 protease Nutrition 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 241000276707 Tilapia Species 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 241000962514 Alosa chrysochloris Species 0.000 claims description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 claims description 2
- 241000252233 Cyprinus carpio Species 0.000 claims description 2
- 241000720946 Hypophthalmichthys molitrix Species 0.000 claims description 2
- 241001275898 Mylopharyngodon piceus Species 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 239000004365 Protease Substances 0.000 abstract description 4
- 239000002184 metal Substances 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 235000013360 fish flour Nutrition 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 235000019419 proteases Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
- A22C—PROCESSING MEAT, POULTRY, OR FISH
- A22C25/00—Processing fish ; Curing of fish; Stunning of fish by electric current; Investigating fish by optical means
- A22C25/16—Removing fish-bones; Filleting fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a method for performing bone and meat separation on fish wastes by utilizing microbial fermentation. The method comprises the following main steps: adding fresh fish wastes and water into a fermentation tank according to the mass ratio of 1:(1-3); introducing bacillus licheniformis HL-3 capable of producing high temperature-resistant proteases into the fermentation tank for fermentation; controlling the fermentation temperature to be 55-60 DEG C and the fermentation time to be 6-12 hours; after the end of fermentation, enabling materials in the fermentation tank to firstly pass through a large-aperture metal mesh screen for filtering and separation so as to obtain intercepted fish bones and then enabling the permeated materials to pass through a small-aperture metal mesh screen for filtering and separation so as to obtain intercepted fish meat. The method for performing the bone and meat separation on the fish wastes, disclosed by the invention, has the characteristics of being convenient and simple to operate and low in cost, thereby having a good application prospect.
Description
Technical field
The present invention relates to a kind of method utilizing fermentable to carry out fish scrap kindred separation, belong to the technical field of processing of aquatic products and utilization.
Background technology
The processing of the current Fish of China is based on preliminary working frozen fillet.In the course of processing of fillet, substantial amounts of fish scrap can be produced.These fish scraps are mainly used to be processed into fish flour at present, but the kindred before fish scrap processing fish flour does not separate, so that the protein content of the fish flour of preparation is low, and this resource of fishbone is the most well utilized, therefore it is badly in need of a kind of simple method and the kindred of fish scrap is separated.
The method that fish scrap kindred separates at present mainly has enzymatic isolation method and mechanical phonograph recorder separation.Enzymatic isolation method mainly adds protease hydrolyzed so that the kindred in fish scrap reaches to separate.But enzymatic isolation method needs to add expensive protease, and cost is high, is difficult to large-scale utilization and extention.Mechanical Method mainly uses deboner to be separated with the flesh of fish by fishbone, but the method for machinery needs to put into expensive equipment, and treating capacity is few, is difficult to large-scale utilization.
Summary of the invention
For the problem existing for the separation of current fish scrap kindred, it is an object of the invention to provide the kindred separation method of a kind of simple fish scrap.
In order to solve the problems referred to above, the technical solution adopted in the present invention is as follows: a kind of method utilizing fermentable to carry out fish scrap kindred separation, comprises the steps:
(1) by slant preservation can produce thermostable proteinase Bacillus licheniformis (Bacillus licheniformis) HL-3 is linked into after sterilizing in the LB fluid medium of cooling, in 55 DEG C, 150r/min cultivate 12h, be prepared as Bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor is standby；
(2) ratio that fresh fish scrap and water are 1:1 ~ 3 in mass ratio is joined in fermentation vat；
(3) toward in step (2) equipped with the fermentation vat of fish scrap accesses prepared by step (1) Bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor, and mix；
(4) fish scrap adding bacterium step (3) obtained ferments in following manner: fermentation temperature controls at 55 ~ 60 DEG C, and in fermentation vat, water surface elevation is 50cm～100cm, and fermentation time is 6h～12h；
(5) after fermentation ends, first carrying out filtering separation by large aperture metallic screen by the material in fermentation vat, trapped substance is fishbone, is the flesh of fish and water through thing；
(6) flesh of fish step (5) passed through and water carry out filtering separation by the metallic screen of a small-bore again, and trapped substance is the flesh of fish.
In described step (1) used can produce thermostable proteinase Bacillus licheniformis (Bacillus licheniformis) HL-3 is preserved in Guangdong Province's Culture Collection on January 18th, 2016, deposit number is GDMCC No:60003, and preservation address is Guangdong Microbes Inst, 5th floors, No. 59 building of compound, Xianlie Middle Road, Guangzhou City 100.
Fish scrap in described step (2) is the one in leftovers of tilapia, morrhua leftover bits and pieces, Cyprinus carpio leftover bits and pieces, Harengulazunasi (Bleeker) leftover bits and pieces, Mylopharyngodon piceus leftover bits and pieces, Hypophthalmichthys molitrix leftover bits and pieces, Ctenopharyngodon idellus leftover bits and pieces and Skipjack leftover bits and pieces.
Bacillus licheniformis in described step (3) (Bacillus licheniformis) HL-3 seed liquor inoculum concentration with access seed liquor quality account for fish scrap gross mass percentages, be 0.01%～5%.
Sieve aperture 2 ~ the 15cm of described step (5) large pore metallic screen.Sieve aperture 0.01 ~ the 2cm of described step (6) small aperture metallic screen.
The present patent application compared with prior art, has the following advantages.
(1) low cost.In this technology, fermentable temperature is consistent with fish scrap endogenous enzymes peak enzymolysis-ability temperature so that fermentable is together with the enzymolysis perfect adaptation of endogenous enzymes.Zymolysis just because of the endogenous enzymes of the fish scrap made full use of so that the slight fermentation of Bacillus licheniformis i.e. can reach the purpose that kindred separates.Compared with separating with existing enzyme process kindred, this technology need not separately add protease, low cost.
(2) easy and simple to handle.With the method for mechanical separation, the equipment that this technology need not be complicated, in a simple fermentation vat.Further, this technology uses a kind of slight fermentation without artificial ventilation, and whole sweat can be simple and easy to control without manual operation.
(3) safety.This technology use high temperature resistant Bacillus licheniformis (Bacillus licheniformis) ferment, at high temperature can suppress the growth of pathogenic bacterium and putrefaction bacteria, thus the technical scheme safety that the present invention provides.
The invention provides a kind of method utilizing fermentable to carry out fish scrap kindred separation.The method that the present invention provides has feature easy and simple to handle and low cost, has a good application prospect.Carried out the kindred separation of fish scrap by the technology of the present invention after, not only can improve the protein content of made fish flour, and fishbone can also be recycled.
Detailed description of the invention
In order to be more fully understood that the present invention, with regard to the selection of fish scrap, fermentation parameter and fermentation mode, the invention will be further described in conjunction with the embodiments below, but protection scope of the present invention is not limited to this.
Embodiment 1: by slant preservation can produce thermostable proteinase Bacillus licheniformis (Bacillus licheniformis) HL-3 is linked into after sterilizing in the LB fluid medium of cooling, in 55 DEG C, 150r/min cultivate 12h, be prepared as Bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor is standby.Joining in fermentation vat by 1000 kilograms of fresh leftovers of tilapia and 2500 kg of water, in fermentation vat, water surface elevation is 60cm；By 2 kilograms of Bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor joins in fermentation vat, and stir evenly；Controlling fermentation temperature is 55 DEG C, and fermentation time is 9h；After fermentation ends, carrying out filtering separation by the metallic screen that aperture is 12 cm by material in fermentation vat, trapped substance is fishbone, through the mixture that thing is the flesh of fish and water；The flesh of fish that will transmit through and water carry out filtering by the metallic screen that aperture is 1 cm again and separate, and trapped substance is the flesh of fish.
Claims (6)
1. one kind utilizes the method that fermentable carries out fish scrap kindred separation, it is characterised in that: the method comprises the steps:
(1) by slant preservation can produce thermostable proteinase Bacillus licheniformis (Bacillus
licheniformis) HL-3 is linked into after sterilizing in the LB fluid medium of cooling, in 55 DEG C, 150r/min cultivate 12h, be prepared as Bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor is standby；
(2) ratio that fresh fish scrap and water are 1:1 ~ 2.5 in mass ratio is joined in fermentation vat；
(3) toward in step (2) equipped with the fermentation vat of fish scrap accesses prepared by step (1) Bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor, and mix；
(4) fish scrap adding bacterium step (3) obtained ferments in following manner: fermentation temperature controls at 55 ~ 60 DEG C, and in fermentation vat, water surface elevation is 50cm～100cm, and fermentation time is 6h～12h；
(5) after fermentation ends, first carrying out filtering separation by large aperture metallic screen by the material in fermentation vat, trapped substance is fishbone, is the flesh of fish and water through thing；
(6) flesh of fish step (5) passed through and water carry out filtering separation by the metallic screen of a small-bore again, and trapped substance is the flesh of fish.
A kind of method utilizing fermentable to carry out fish scrap kindred separation the most according to claim 1, its feature exists
In: Bacillus licheniformis used in described step (1) (Bacillus
licheniformis) HL-3 is GDMCC No:60003 at the deposit number of Guangdong Province's Culture Collection.
A kind of method utilizing fermentable to carry out fish scrap kindred separation the most according to claim 1, its feature exists
In: the fish scrap in described step (2) is the one in leftovers of tilapia, morrhua leftover bits and pieces, Cyprinus carpio leftover bits and pieces, Harengulazunasi (Bleeker) leftover bits and pieces, Mylopharyngodon piceus leftover bits and pieces, Hypophthalmichthys molitrix leftover bits and pieces, Ctenopharyngodon idellus leftover bits and pieces and Skipjack leftover bits and pieces.
A kind of method utilizing fermentable to carry out fish scrap kindred separation the most according to claim 1, its feature exists
In: Bacillus licheniformis in described step (3) (Bacillus licheniformis) HL-3 seed liquor inoculum concentration with access seed liquor quality account for fish scrap gross mass percentages, be 0.01%～5%.
A kind of method utilizing fermentable to carry out fish scrap kindred separation the most according to claim 1, its feature exists
In: the sieve aperture of described step (5) large pore metallic screen is 2 ~ 15cm.
A kind of method utilizing fermentable to carry out fish scrap kindred separation the most according to claim 1, its feature exists
In: the sieve aperture of described step (6) small aperture metallic screen is 0.01 ~ 2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610129692.9A CN105724550B (en) | 2016-03-09 | 2016-03-09 | A method of carrying out fish scrap bone and flesh separation using microbial fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610129692.9A CN105724550B (en) | 2016-03-09 | 2016-03-09 | A method of carrying out fish scrap bone and flesh separation using microbial fermentation |
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| Publication Number | Publication Date |
|---|---|
| CN105724550A true CN105724550A (en) | 2016-07-06 |
| CN105724550B CN105724550B (en) | 2018-08-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610129692.9A Expired - Fee Related CN105724550B (en) | 2016-03-09 | 2016-03-09 | A method of carrying out fish scrap bone and flesh separation using microbial fermentation |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107467674A (en) * | 2017-06-22 | 2017-12-15 | 乐清瑞耀食品技术有限公司 | Compound based on duckbilled fish active peptides |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3804964A (en) * | 1970-06-04 | 1974-04-16 | Astra Nutrition Ab | Method and apparatus for treating fish |
| CN101248821A (en) * | 2007-11-19 | 2008-08-27 | 广东海洋大学 | Process engineering for extracting fishes processing offal fish oil by enzymolysis process |
| CN101705219A (en) * | 2009-11-12 | 2010-05-12 | 集美大学 | Production method of protease hydrolyzing leftovers of freshwater fish and other fish efficiently |
| CN102150875A (en) * | 2011-01-21 | 2011-08-17 | 河南农业大学 | Enzymolysis method for bone dregs |
| CN102550804A (en) * | 2012-02-04 | 2012-07-11 | 广东海洋大学 | Method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals |
| CN102792997A (en) * | 2012-08-10 | 2012-11-28 | 中国水产科学研究院东海水产研究所 | Method for separating shell and meat of small crustacean |
| CN104844292A (en) * | 2015-04-29 | 2015-08-19 | 漳州三本肥料工业有限公司 | Method for preparing fertilizer by using shrimp wastes and product thereof |
| KR101563109B1 (en) * | 2015-04-17 | 2015-10-23 | 황미경 | A mudfish bone and fish meat separation equipment make use of centrifugal force |
| CN105211616A (en) * | 2015-10-27 | 2016-01-06 | 广东海洋大学 | A kind of method of comprehensive utilization to shrimping beam trawl |
-
2016
- 2016-03-09 CN CN201610129692.9A patent/CN105724550B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3804964A (en) * | 1970-06-04 | 1974-04-16 | Astra Nutrition Ab | Method and apparatus for treating fish |
| CN101248821A (en) * | 2007-11-19 | 2008-08-27 | 广东海洋大学 | Process engineering for extracting fishes processing offal fish oil by enzymolysis process |
| CN101705219A (en) * | 2009-11-12 | 2010-05-12 | 集美大学 | Production method of protease hydrolyzing leftovers of freshwater fish and other fish efficiently |
| CN102150875A (en) * | 2011-01-21 | 2011-08-17 | 河南农业大学 | Enzymolysis method for bone dregs |
| CN102550804A (en) * | 2012-02-04 | 2012-07-11 | 广东海洋大学 | Method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals |
| CN102792997A (en) * | 2012-08-10 | 2012-11-28 | 中国水产科学研究院东海水产研究所 | Method for separating shell and meat of small crustacean |
| KR101563109B1 (en) * | 2015-04-17 | 2015-10-23 | 황미경 | A mudfish bone and fish meat separation equipment make use of centrifugal force |
| CN104844292A (en) * | 2015-04-29 | 2015-08-19 | 漳州三本肥料工业有限公司 | Method for preparing fertilizer by using shrimp wastes and product thereof |
| CN105211616A (en) * | 2015-10-27 | 2016-01-06 | 广东海洋大学 | A kind of method of comprehensive utilization to shrimping beam trawl |
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| CN105724550B (en) | 2018-08-03 |
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