CN104719636A - Method for preparing feed antibiotic substitute by using aquatic product processing wastewater and Chinese herbal medicine - Google Patents
Method for preparing feed antibiotic substitute by using aquatic product processing wastewater and Chinese herbal medicine Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for preparing a feed antibiotic substitute by using aquatic product processing wastewater and a Chinese herbal medicine. The method comprises the following steps: evaporating and concentrating the fresh aquatic product processing wastewater till the protein content is 18-25% for later use, smashing the Chinese herbal medicine and passing 60-mesh for later use; mixing the concentrated aquatic product processing wastewater and the smashed Chinese herbal medicine, adding a nutriment and mixing uniformly to form a fermentation culture medium; sterilizing and cooling the fermentation culture medium, inoculating bacillus subtilis HL-1 for producing subtilin and performing solid aerobic fermentation; drying, smashing and packaging the fermented product to prepare the feed antibiotic substitute product. By adopting the method, the aquatic product processing wastewater is changed into valuable things, and the feed antibiotic substitute containing the subtilin, the Chinese herbal medicine effective constituents and a nutrient small peptide is prepared; the feed antibiotic substitute has good growth promotion and a disease-resistant function, can be used for completely substituting the feed antibiotic in the feed industry and has good application prospect and social significance.
Description
Technical field
The present invention relates to a kind of method utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare feeding antibiotic substitute, belong to technical field prepared by feed addictive.
Background technology
In recent years along with the fast development of China's Aquatic Product Process Industry, one of the aquatic products processing enterprises that is treated as of aquatic products processing wastewater is born greatly.Waste water mainly comes from material cooking in processing of aquatic products process, thaw and the operation such as cleaning, the features such as have organic concentration high, the larger molecular organics such as protein, grease matter is many, and biodegradation rate is slow.Particularly fish meal processing waste water, prawn waste water from cooking and chitin processing waste waters etc., the protein content 3% ~ 10% of these waste water, is difficult to process especially.The current process for aquatic products processing wastewater adopts the method for biochemical treatment more, and these methods not only do not recycle the protein resource in waste water, cause the waste of a large amount of high-quality protein resource, and processing cost are high.
There are 9.7 ten thousand tons for livestock breeding industry in about 210,000 tons of antibiotic raw materials that current China produces every year, account for gross annual output amount 46.1%.Excessive use antibiotic not only result in the drug resistance of cultivated animals to antibiotic medicine, the resistance against diseases of cultivated animals is declined, and these antibiotic can enter human body with food chain, human body is made inadvertently to produce drug-resistant bacteria, treatment difficulty and expense will be increased once sick, even likely can produce " superbacteria ", occur situation about pasting medical help.Along with improving constantly of people's quality of life, safe and sanitary varieties of food items being comprised to meat product has had higher requirement, also day by day strong to the cry of " quality-assured meat ", " pollution-free food ".Therefore, finding antibiotic green substitute is China's feed and problem demanding prompt solution in cultivation industry development.
Chinese herbal medicine is the rarity in Chinese medicine treasure-house, and Chinese herbal medicine is as feed addictive, starts in Ancient Times in China.Chinese herbal medicine has and has no side effect, not easily causes the characteristic such as resistance, noresidue, and the green substitute as a kind of feeding antibiotic has been widely recognized at feed industry.From the eighties in 20th century, Chinese herbal medicine has been used to make feeding antibiotic substitute, and achieves certain effect.After Chinese herbal medicine undergoes microbial fermentation, its active ingredient is fully discharged, and makes it have more biologically active and curative effect study hotspot especially in recent years.China's feed (2010,419(15): 13-17) document discloses and from different herbal raw material, is separated the aspergillus oryzae obtained ferments to Chinese herbal medicine, and the bacteriostatic activity of fermentation material cooking liquor to Escherichia coli, staphylococcus aureus, proteus vulgaris and salmonella typhimurium comparatively contrasts raising 36.03%, 60.83%, 27.64%, 41.32% respectively.But be just confined to fully allow Chinese herbal medicine effective ingredients discharge to the fermentation of Chinese herbal medicine above, thus improve bacteriostatic activity, but the antibacterial activity of Chinese herbal medicine effective ingredients does not promote.It is that using dosage is bigger than normal that current fermentation of Chinese herbal medicine exists maximum problem.This not only adds the use cost of product, but also affect feed nutrition proportioning and palatability, thus fermentation of Chinese herbal medicine is not still widely used at feed industry.Therefore, be badly in need of the antibacterial activity increasing substantially active ingredient in fermentation of Chinese herbal medicine, reduce the using dosage of fermentation of Chinese herbal medicine at feed industry, to promote that fermentation of Chinese herbal medicine is in the extensive use of feed industry.
Bacteriocin is the albumen with antibacterial action that produced by microorganism of a class or polypeptide, can suppress gram-positive bacteria, Gram-negative bacteria and fungi.Due to bacteriocin have free from environmental pollution, pathogenic bacteria can not be made to produce drug resistance, strengthen the advantage such as host immunity, be acknowledged as a kind of natural bacteriostatic agent.But at present, because high production cost makes the application of bacteriocin mainly at field of food, and little in the application of feedstuff industry.The direct use of bacteriocin on food, also illustrate that bacteriocin is safe to animals and humans.Therefore be badly in need of carrying the antibacterial activity of bacteriocin and its production cost of reduction, to promote that bacteriocin is in the application of feed industry.
Within 1988, nisin obtains the accreditation of FDA first as food additives, has and is using nisin as food preservative more than 100 countries and regions, thus facilitates other bacterial species element and the research at other field.But at present the research of bacteriocin focuses mostly in bacteriocin lab aspect, and in the world to bacillus subtilis (
bacillus subtilis) research of subtilin that produces is then little, domestic rare especially.Industrial microorganism (2008,38(4): 14 ~ 18) disclose a kind of bacillus subtilis (
bacillus subtilis) FB123 produces the liquid fermentation condition of subtilin, but the solid state fermentation of the antibacterial activity and subtilin that improve subtilin is produced also not studies have reported that.
Summary of the invention
The present invention for raw material, prepares a kind of compound feeding antibiotic substitute being rich in Chinese herbal medicine effective ingredients and subtilin with aquatic products processing wastewater and Chinese herbal medicine.The object of the invention is not only will to reduce the production cost that subtilin and Chinese herbal medicine ferment, but also the antibacterial activity of Chinese herbal medicine effective ingredients and subtilin will be improved, thus reduce subtilin and the Chinese herbal medicine using dosage at feed industry, to promote that subtilin and Chinese herbal medicine are in the application widely of feed industry.Another object of the present invention is turned waste into wealth by aquatic products processing wastewater, fully reclaims the protein resource in aquatic products processing wastewater.
In order to solve the problem, the technical solution adopted in the present invention is as follows: a kind of method utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare feeding antibiotic substitute, comprises the steps:
(1) by slant preservation can produce subtilin bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use;
(2) be 18% ~ 25% for subsequent use by aquatic products processing wastewater evaporation and concentration to protein content, cross 60 mesh sieves after being pulverized by Chinese herbal medicine for subsequent use;
(3) ratio being 0.8 ~ 1.5:1 in mass ratio by the Chinese herbal medicine crossing 60 mesh sieves after concentrated rear aquatic products processing wastewater obtained for step (2) and pulverizing mixes, and adds nutrients mixing, the fermentation medium of acquisition mixing;
(4) fermented and cultured of mixing step (3) obtained is based on 121 DEG C of sterilizing 30min;
(5) fermentation medium after sterilizing step (4) obtained is cooled to room temperature, then access bacillus subtilis prepared by step (1) (
bacillus subtilis) HL-1 seed liquor, and mix;
(6) fermentation of raw material: the fermentation medium adding bacterium that step (5) obtains is fermented according to following method: fermentation medium is divided in the fermentation tray of having sterilized, charging thickness is 4cm ~ 8cm, the sterilized wet gauze of fermentation medium surface coverage in tray, again tray is placed in the shelf top fermentation in sterilized solid fermentation room, the temperature that controls environment is at 28 DEG C ~ 30 DEG C, and fermentation time is 35h ~ 45h;
(7) product handling system: fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, has namely obtained a kind of feeding antibiotic substitute finished product utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare.
Bacillus subtilis used in described step (1) (
bacillus subtilis) HL-1 can produce subtilin, this bacterium is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 20th, 2015, deposit number is CGMCC No.10379, and preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
Aquatic products processing wastewater in described step (2) is the one in the rotten processing waste water of fish meal processing waste water, the alkali-wash waste water of chitin process, prawn waste water from cooking and fish.Chinese herbal medicine in described step (2) is a kind of or wherein two or more mixture in Radix Glycyrrhizae, the capsule of weeping forsythia, Asiatic plantain, perillaseed, the fruit of Chinese magnoliavine, Verbena officinalis.
The mixture that nutrients in described step (3) is glucose, frerrous chloride and dipotassium hydrogen phosphate form; Nutraceutical addition in described step (3) accounts for fermentation medium gross mass percentages with the nutraceutical quality of adding, and is respectively: glucose 0.01% ~ 5%, frerrous chloride 0.01% ~ 5%, dipotassium hydrogen phosphate 0.01% ~ 5%.
The middle bacillus subtilis of described step (5) (
bacillus subtilis) inoculum concentration of HL-1 seed liquor accounts for fermentation medium gross mass percentages with the quality accessing seed liquor, is 0.1% ~ 5%.
The present invention compares with existing technology, has the following advantages.
(1) production cost of the green substitute of feeding antibiotic is reduced.The present invention with aquatic products processing wastewater and Chinese herbal medicine for raw material, with can produce subtilin bacillus subtilis (
bacillus subtilis) HL-1 carries out solid state fermentation, not only can ferment generation subtilin, but also medium-height grass the effective elements of the medicine is discharged.The fermentation of subtilin and the fermentation of Chinese herbal medicine unite two into one by the present invention, thus reduce the production cost of the green substitute of feeding antibiotic on the whole.
(2) improve the antibacterial activity of subtilin and Chinese herbal medicine effective ingredients, thus significantly reduce the using dosage of subtilin and Chinese herbal medicine effective ingredients.The present invention have selected from thousands of medium-height grass Drug Storage top sieve the Chinese herbal medicine that its active ingredient and subtilin have collaborative inhibitory effect.The fermentation generation of subtilin and Chinese herbal medicine are not only fermented and are united two into one by the present invention, and due to the interaction of subtilin and Chinese herbal medicine effective ingredients, the antibacterial activity of the two all significantly strengthens.This is also the fermentation of single at present Chinese herbal medicine with not available for subtilin fermenting and producing.Just because of invention increases prepared subtilin and the antibacterial activity of Chinese herbal medicine effective ingredients, thus the using dosage of the feeding antibiotic substitute prepared by the present invention is greatly reduced, decrease several times to tens times when wherein contained subtilin is used alone with the actual using dosage ratio of Chinese herbal medicine effective ingredients.
(3) aquatic products processing wastewater is turned waste into wealth, recycled the protein resource in waste water.Compared with existing biochemical processing method, aquatic products waste water is turned waste into wealth by the present invention, has recycled the protein resource in waste water.Not only so, protein degradation, also by the fermentation of microorganism, is little peptide by the present invention, improves the nutritive value of protein.
(4) improve growth-promoting effect.In method provided by the invention, the protein in aquatic products processing wastewater is by being degraded into little peptide under the fermentation of microorganism.Little peptide has growth promoting function to letting animals feed, and this has obtained the accreditation of feed industry.Be rich in little peptide, can further improve the growth-promoting effect of feeding antibiotic substitute prepared by the inventive method.
The invention provides the preparation method of the feed addictive of a kind of aquatic products processing wastewater and Chinese herbal medicine mixed culture fermentation.The present invention not only reduces the production cost of the green substitute of feeding antibiotic; But also greatly reduce the using dosage of subtilin and Chinese herbal medicine effective ingredients; And further increase the growth-promoting effect of feeding antibiotic substitute.The culture experiment of the pig of feeding antibiotic substitute in South China of what the present invention obtained be rich in subtilin, Chinese herbal medicine effective ingredients and little peptide, chicken, prawn shows, the feeding antibiotic substitute prepared by the inventive method can substitute feeding antibiotic completely, obvious reduction aquaculture cost, obtains significant economic benefit.
Detailed description of the invention
In order to understand the present invention better, with regard to the selection of the kind of aquatic products processing wastewater and Chinese herbal medicine, fermentation parameter and fermentation mode, the invention will be further described in conjunction with the embodiments below, but protection scope of the present invention is not limited thereto.
Embodiment 1: by slant preservation can produce subtilin bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use.It is 25% for subsequent use for fish meal processing waste water heating evaporation being concentrated into protein content; 60 mesh sieves are crossed for subsequent use after being pulverized by Radix Glycyrrhizae.150 kilograms of concentrated fish meal processing waste waters and 100 kilograms are pulverized and cross 60 mesh sieves Radix Glycyrrhizae mixing, and add 500 grams of glucose, 150 grams of frerrous chlorides mix together with 600 grams of dipotassium hydrogen phosphates, obtain mixing after fermentation medium; Fermented and cultured after mixing is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1 kilogram of bacillus subtilis (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal fermentation medium is divided in the fermentation tray of the alcohol surface sterilization using 75%, thickness of feeding in tray is 8 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is 28 DEG C, and fermentation time is 45h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain a kind of feeding antibiotic substitute finished product utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare of the present invention.
Embodiment 2: by slant preservation can produce subtilin bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use.It is 25% for subsequent use for fish meal processing waste water heating evaporation being concentrated into protein content; 60 mesh sieves are crossed for subsequent use after Radix Glycyrrhizae and the fruit of Chinese magnoliavine being pulverized.100 kilograms of concentrated fish meal processing waste waters and 100 kilograms are pulverized and cross 60 mesh sieves composite Chinese herbal (75 kilograms of Radix Glycyrrhizaes and 25 kilograms of fruit ofs Chinese magnoliavine) mixing, and add 400 grams of glucose, 200 grams of frerrous chlorides mix together with 425 grams of dipotassium hydrogen phosphates, obtain mixing after fermentation medium; Fermented and cultured after mixing is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1 kilogram of bacillus subtilis (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, thickness of feeding in tray is 4 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is 30 DEG C, and fermentation time is 35h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain a kind of feeding antibiotic substitute finished product utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare of the present invention.
Embodiment 3: by slant preservation can produce subtilin bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use.The alkali-wash waste water of chitin process is added hydrochloric acid and is adjusted to 7.0, and waste water heating evaporation to be concentrated into protein content be 18% for subsequent use; 60 mesh sieves are crossed for subsequent use after being pulverized by the capsule of weeping forsythia.80 kilograms of concentrated waste water and 100 kilograms are pulverized and cross 60 mesh sieves the capsule of weeping forsythia mixing, and add 600 grams of glucose, 150 grams of frerrous chlorides mix together with 450 grams of dipotassium hydrogen phosphates, obtain mixing after fermentation medium; Mixed fermented and cultured is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1 kilogram of bacillus subtilis (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, thickness of feeding in tray is 6 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is at 29 DEG C, and fermentation time is 40h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain a kind of feeding antibiotic substitute finished product utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare of the present invention.
Embodiment 4: by slant preservation can produce subtilin bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use.It is 20% for subsequent use for prawn waste water from cooking heating evaporation being concentrated into protein content; By Asiatic plantain and broken rear mistake 60 mesh sieve of perillaseed for subsequent use.90 kilograms of concentrated waste water and 100 kilograms are pulverized and cross 60 mesh sieves composite Chinese herbal (60 kilograms of Asiatic plantains+40 kilograms of perillaseeds) mixing, and add 400 grams of glucose, 150 grams of frerrous chlorides mix together with 525 grams of dipotassium hydrogen phosphates, obtain mixing after fermentation medium; Mixed fermented and cultured is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1 kilogram of bacillus subtilis (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, thickness of feeding in tray is 5 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is at 30 DEG C, and fermentation time is 38h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain a kind of feeding antibiotic substitute finished product utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare of the present invention.
Embodiment 5: by slant preservation can produce subtilin bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use.It is 18% for subsequent use for rotten for fish processing waste water heating evaporation processed being concentrated into protein content; 60 mesh sieves are crossed for subsequent use after Radix Glycyrrhizae, Asiatic plantain and the fruit of Chinese magnoliavine being pulverized.100 kilograms of concentrated waste water and 100 kilograms are pulverized and cross 60 mesh sieves composite Chinese herbal (35 kilograms of Radix Glycyrrhizaes+35 kilograms of Asiatic plantains+30 kilograms of fruit ofs Chinese magnoliavine) mixing, and add 488 grams of glucose, 118 grams of frerrous chlorides mix together with 588 grams of dipotassium hydrogen phosphates, obtain mixing after fermentation medium; Mixed fermented and cultured is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1.5 kilograms of bacillus subtilises (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, thickness of feeding in tray is 4 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is at 30 DEG C, and fermentation time is 38h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain a kind of feeding antibiotic substitute finished product utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare of the present invention.
Claims (7)
1. utilize aquatic products processing wastewater and Chinese herbal medicine to prepare a method for feeding antibiotic substitute, it is characterized in that: the method comprises the steps:
(1) in the LB fluid nutrient medium cooled after the bacillus subtilis HL-1 that can produce subtilin of slant preservation being linked into sterilizing, in 37 DEG C, 150r/min cultivates 16h, is prepared into bacillus subtilis HL-1 seed liquor for subsequent use;
(2) be 18% ~ 25% for subsequent use by aquatic products processing wastewater evaporation and concentration to protein content, cross 60 mesh sieves after being pulverized by Chinese herbal medicine for subsequent use;
(3) ratio being 0.8 ~ 1.5:1 in mass ratio by the Chinese herbal medicine crossing 60 mesh sieves after concentrated rear aquatic products processing wastewater obtained for step (2) and pulverizing mixes, and adds nutrients mixing, the fermentation medium of acquisition mixing;
(4) fermented and cultured of mixing step (3) obtained is based on 121 DEG C of sterilizing 30min;
(5) fermentation medium after sterilizing step (4) obtained is cooled to room temperature, then accesses the bacillus subtilis HL-1 seed liquor prepared by step (1), and mixes;
(6) fermentation of raw material: the fermentation medium adding bacterium that step (5) obtains is fermented according to following method: fermentation medium is divided in the fermentation tray of having sterilized, charging thickness is 4cm ~ 8cm, the sterilized wet gauze of fermentation medium surface coverage in tray, again tray is placed in the shelf top fermentation in sterilized solid fermentation room, the temperature that controls environment is at 28 DEG C ~ 30 DEG C, and fermentation time is 35h ~ 45h;
(7) product handling system: fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, has namely obtained a kind of feeding antibiotic substitute finished product utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare.
2. a kind of method utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare feeding antibiotic substitute according to claim 1, is characterized in that: bacillus subtilis HL-1 used in described step (1) is CGMCC No.10379 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.
3. a kind of method utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare feeding antibiotic substitute according to claim 1, is characterized in that: the aquatic products processing wastewater in described step (2) is the one in the rotten processing waste water of fish meal processing waste water, the alkali-wash waste water of chitin process, prawn waste water from cooking and fish.
4. a kind of method utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare feeding antibiotic substitute according to claim 1, is characterized in that: the Chinese herbal medicine in described step (2) is a kind of or wherein two or more mixture in Radix Glycyrrhizae, the capsule of weeping forsythia, Asiatic plantain, perillaseed, the fruit of Chinese magnoliavine, Verbena officinalis.
5. a kind of method utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare feeding antibiotic substitute according to claim 1, is characterized in that: the mixture that the nutrients in described step (3) is glucose, frerrous chloride and dipotassium hydrogen phosphate form.
6. a kind of method utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare feeding antibiotic substitute according to claim 1, it is characterized in that: the nutraceutical addition in described step (3) accounts for fermentation medium gross mass percentages with the nutraceutical quality of adding, be respectively: glucose 0.01% ~ 5%, frerrous chloride 0.01% ~ 5%, dipotassium hydrogen phosphate 0.01% ~ 5%.
7. a kind of method utilizing aquatic products processing wastewater and Chinese herbal medicine to prepare feeding antibiotic substitute according to claim 1, it is characterized in that: in described step (5), the inoculum concentration of bacillus subtilis HL-1 seed liquor accounts for fermentation medium gross mass percentages with the quality accessing seed liquor, is 0.1% ~ 5%.
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| CN201510092623.0A CN104719636A (en) | 2015-03-02 | 2015-03-02 | Method for preparing feed antibiotic substitute by using aquatic product processing wastewater and Chinese herbal medicine |
| CN201610106542.6A CN105685475A (en) | 2015-03-02 | 2016-02-27 | Method for preparing feed antibiotic substitute from subtilin producing bacillus subtilis fermented Chinese herbal medicine |
| CN201610112568.1A CN105685477A (en) | 2015-03-02 | 2016-03-01 | Preparation method of antibiotic substitute rich in subtilin and effective Chinese herbal medicine ingredients for feeding |
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| CN201610106542.6A Pending CN105685475A (en) | 2015-03-02 | 2016-02-27 | Method for preparing feed antibiotic substitute from subtilin producing bacillus subtilis fermented Chinese herbal medicine |
| CN201610112568.1A Pending CN105685477A (en) | 2015-03-02 | 2016-03-01 | Preparation method of antibiotic substitute rich in subtilin and effective Chinese herbal medicine ingredients for feeding |
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| CN102094054B (en) * | 2010-12-07 | 2013-04-10 | 南京农业大学 | Production method of Bacillus subtilis antimicrobial lipopeptide and application of Bacillus subtilis antimicrobial lipopeptide in piglet feeds |
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| CN104719635A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN104719636A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed antibiotic substitute by using aquatic product processing wastewater and Chinese herbal medicine |
| CN105010730A (en) * | 2015-08-21 | 2015-11-04 | 何万领 | Preparation method of probiotic fermented Chinese traditional herb feed additive for laying hens |
| CN105211634A (en) * | 2015-10-13 | 2016-01-06 | 延川县富源康茶业专业合作社 | A kind of regulant for animal's growth and preparation method thereof |
| CN105211616B (en) * | 2015-10-27 | 2020-01-07 | 广东海洋大学 | Comprehensive utilization method of prawn leftovers |
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| CN105746892A (en) * | 2016-03-09 | 2016-07-13 | 广东海洋大学 | Method of utilizing solid-state fermentation of aquatic product protein to prepare green growth-promoting feed additive |
| CN105661013B (en) * | 2016-03-09 | 2019-11-19 | 广东海洋大学 | A kind of method that fermentation of Chinese herbal medicine prepares compound feed additive for promoting growth |
| CN106071391A (en) * | 2016-06-17 | 2016-11-09 | 合肥丰瑞隆生物科技有限公司 | Feed additive of cultivation Mugil cephalus and preparation method thereof |
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| Publication number | Publication date |
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| CN105685475A (en) | 2016-06-22 |
| CN105685477A (en) | 2016-06-22 |
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