CN106472962A - The detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its preparation technology in a kind of biodegradation feedstuff - Google Patents
The detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its preparation technology in a kind of biodegradation feedstuff Download PDFInfo
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Abstract
The present invention is the detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and preparation technology in a kind of biodegradation feedstuff, and detoxifying agent includes：Bentonite 40～60；Immune polysaccharide 20；Free radical scavenger 10～20；The bacillus subtilis powder agent 20～30 of degrading zearalenone；The step of preparation bacillus subtilis powder agent：1) original strain carries out seed liquor activation, carries out kind of a female culture in the one grade fermemtation tank hold culture medium；2）Plant female culture fluid to be amplified cultivating in the second order fermentation tank holding LB culture medium；3）Thalline and fermentation liquid are carried out separating, concentrate, be dried, makes the bacillus subtilis powder agent of degrading zearalenone；Mixture compounds and obtains finished product twice for powder and above-mentioned weight portion., to the degradation rate of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone up to more than 95%, degradation efficiency is high for the present invention, it is to avoid the defect of adsorbent, and adds immune polysaccharide in product, removes free based component, can play hepatoprotective, the function of raising immunity of organisms, effect is obvious.
Description
Technical field
The invention belongs to feed additive technology, degraded in animal feed by biotechnology more particularly, to a kind of
The detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its this detoxifying agent preparation technology.
Background technology
Mycotoxins in feed：The secondary metabolite that mycete produces, to the speed of growth of animal, organ, reproductive ability
Energy, production performance etc. are negatively affected.
6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone：There is estrogen action, mainly act on reproductive system, domestic animal can be made, poultry produces female
The hyperfunction disease of hormone.The feedstuff that female animal animal (including people) eats containing 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone can cause miscarriage, stillborn fetuses and monster；
Mold toxin sorbent：Part, a small amount of, particular toxin are fallen by physics mode absorption, excretes, poison will not be changed
Disposition shape and chemical constitution；
China's contamination of raw material ratio is more serious at present, and the pollution of mycotoxin has been a global problem, according to the United Nations grain farmer
Survey of organization, every year by the corn of fungal contamination, oilseed and feedstuff account for the 10% about of its total amount in the whole world, and according to U.S.
The investigation of state, in the corn of all world trade supply, has 25% to be polluted by mycotoxin.Mycotoxin seriously threatens dynamic
The production performance of thing and human health, bring huge economic loss to every year food industry, feed industry and animal husbandry.Due to
Physics and chemical method remove mycotoxin and there are a variety of applied defects, and current biodegradation has become safe efficient and environmental protection
Detoxification and receive much concern.
At present detoxicating mycotoxin being processed mainly has：Mechanical detoxification, biological detoxication, chemical detoxication and physical absorption detoxification
Etc. method.Wherein, mechanical detoxification, chemical detoxication are because of trivial operations, with high costs, itself detoxification technology defect and chemicals
The problems such as residual, effectively cannot use in production practices；Physical absorbent detoxicity method mainly passes through to add in feedstuff or corn
Plus various adsorbent, reduce or eliminate mycotoxin and absorb in gastrointestinal, adding in feedstuff can be with absorbing mycotoxin
Material, so that toxin is not absorbed by animals when through animal intestinal, be expelled directly out animal external.This is current feed market
On a kind of more ripe and more feasible detoxicating mycotoxin method, it is contemplated that the multiformity of mycotoxin structure, special
Property and the limitation of unicity, common adsorbents material, it is multiple that any single, conventional adsorbent material is all unable to effectively solving
The pollution of mycotoxin and harm problem.Mold toxin sorbent widely studied at present includes clay class adsorbent or organic
Polymer, but just at present using situation from the point of view of, effect is all undesirable.
Solve the problems, such as mycotoxin in the market substantially based on adsorbent, main component is essentially montmorillonite, ferment
Blast cell wall polysaccharide.Ultimate principle is the physical absorption using the two, part toxin absorption heel row is removed external, but adsorbent
Shortcoming be can only absorbed portion toxin it is impossible to thoroughly solve the harm that mycotoxin brings；In addition adsorbent is in absorbing toxin
Simultaneously also can nutrient substance in adsorption feed and additive medicine, the production performance of animal is negatively affected, impact is supported
The economic benefit of the person of growing.
Zearalenone toxin has estrogen action, mainly acts on reproductive system, can make domestic animal, and poultry produces female
The hyperfunction disease of gonadal hormone.The feedstuff that female animal animal (including people) eats containing 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone can cause miscarriage, stillborn fetuses and monster.
The current method removing 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone is mainly physical adsorption process, removes the jade in feedstuff using adsorbent
Zearlenone, not only effect is undesirable, and some little points in meeting adsorption feed while adsorbing 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone
Sub- nutrient substance, causes feed nutrition unbalance, affects digesting and assimilating of feedstuff.Additionally, using biological enzyme formulation in actual production
6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone in removal feedstuff is although effect is permissible, but the activity of enzyme preparation is easily subject to some factors extraneous
Some microbial ecological agent in interference, such as temperature, humidity and feedstuff, and enzyme preparation is during feed granulating, often
Substantially reduce its activity, affect its application in animal productiong.
Biological detoxication is a kind of new detoxicating mycotoxin mode, mainly has enzymatic isolation method and microbe fermentation method.Enzymolysis
Method mainly selects some enzymes, using its degraded or destruction toxin.But such method due to mycotoxin species more it is desirable to enzyme
System is complicated complete, and is easily affected by animal digestion organ dysfunction；Additionally, due to enzyme thermo-labile and during feed manufacturing hold
Easy in inactivation, and relatively costly it is also difficult to promote the use of in actual production.To remove mycotoxin using fermentable has
Many reports, are other products such as by mycotoxin microbe conversion, but this conversion are typically slow, and be incomplete
, and the safety of converted product there is no final conclusion, and can play a role in actual production also needs to make the research of system perfecting.
Therefore, research one kind safely, effectively, can be effectively reduced the harm to animal for the mycotoxin, and growth of animal not caused
Negative effect, to medicine and the no adsorbing degrading mold toxin adsorbent of other nutrient substance, is a great prospect
Research topic.
Content of the invention
The invention aims to overcoming the shortcomings of existing adsorbent, a kind of energy is provided to pass through in biodegradation feedstuff
6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone, enhancing animal body resistance, adjustment intestinal health, effectively prevention, elimination 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone are to animal
The feed additive of harm and its preparation technology.
For realizing the purpose of the present invention, the present invention provides a kind of novel corn zeranol detoxification treatment technology, by life
Thing fermentation technique, the fermentation of bacillus subtilis being possible to decompose 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone forms product, and bacillus subtilises enter
The internal enzyme producing decomposition toxin, to 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone biodegradation to reach the purpose dispelling toxin.
The technical scheme is that：
The detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone in a kind of biodegradation feedstuff, described detoxifying agent by following component weight portion compound and
Become：
Bentonite 40～60；
Immune polysaccharide 20；
Free radical scavenger 10～20；
Bacillus subtilis powder agent 20～30；
Wherein, described bacillus subtilis powder agent is the powder made of bacillus subtilises of degrading zearalenone.
In the biodegradation feedstuff of the present invention, the preparation technology of the detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone includes step：
1) the bacillus subtilises original strain of the degrading zearalenone preserving milk powder in test tube is connect by 2% inoculum concentration
Plant in the triangular flask holding LB culture medium, and in 37 DEG C, under conditions of 200r/min, culture 24h carries out seed liquor activation, will
The seed liquor having activated is inoculated in the one grade fermemtation tank hold culture medium with 2% inoculum concentration and carries out kind of a female culture, is fermenting
Uninterruptedly filtrated air is transported in fermentation tank by air compressor in journey；
2）Female culture fluid of planting of fermentation in one grade fermemtation tank is inputted the second order fermentation tank holding LB culture medium by sterile pipes
It is amplified cultivating, the pH value adjusting culture medium in fermentation tank is 8.0 ± 0.5, and is uninterruptedly passed through no by air compressor
Bacterium air, adjusts fermentation temperature and is 37 ± 2 DEG C, rotating speed is 200r/min, so that probioticss is fermented and maximizes；
3）By being centrifuged at a high speed, thalline and fermentation liquid are carried out separating by device, pass through sterile tube by separating the fermentation liquid obtaining
Road, is transported to exchange resin tower and is concentrated, and the fermentation liquid after concentration passes through spray drying tower, and by bacterium solution, fall is made in drying rapidly
The bacillus subtilis powder agent of solution 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone；
4）The free radical scavenger of the immune polysaccharide of 20 weight portions, 10～20 weight portions is carried out mixing for the first time, obtains once
Mixture；
5）The bacillus subtilis powder agent adding 20～30 weight portion fermentations in once mixture carries out second mixing, obtains
Secondary mixture；
6）Add 40～60 parts by weight of bentonite as carrier in second mixture, that is, obtain finished product.
The LB culture medium prescription of described one grade fermemtation tank and second order fermentation tank and collocation method are:Tryptone 10g, chlorination
Sodium 10g, yeast extract 5g, with distilled water constant volume to 1000mL, adjust pH=7.4, after sealing, in 121 DEG C, autoclaving
20min.
Carrying out kind of a percentage by weight for the medium component of female culture in described one grade fermemtation tank is：Wheat bran 80%, rice husk
15%th, bean cake 4.95% and sulfate 0.05＆, material-water ratio, 1:1.1.
Described filtrated air is sterilized using steam autoclave.
The bacillus subtilises of degrading zearalenone toxin are combined by the present invention, and bacillus cereuss enter internal producing and degrade
The enzyme of toxin, mycotoxin is digested into innocuous substance and reaches the purpose removing toxin, and can continue producing enzyme in vivo, produce
Enzyme amount is big, and degradation efficiency is high；And bacillus cereuss are high temperature resistant, acidproof, antibiotic-resistant, strong stress resistance.So with bacillus cereuss
Form joint product advantage is larger.The enzyme of degraded toxin is proposed to make the strong stress resistance of product, low cost, and enzyme, high temperature resistant.
, to the degradation rate of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone up to more than 95%, degradation efficiency is high the present invention, it is to avoid lacking of adsorbent
Fall into, and add immune polysaccharide in product, remove free based component, hepatoprotective, the function of raising immunity of organisms can be played,
Effect is obvious.
Brief description
Fig. 1 is the process chart of detoxifying agent of the present invention.
Specific embodiment
For making the object, technical solutions and advantages of the present invention become more apparent, below in conjunction with the drawings and specific embodiments,
The present invention is described in more detail.
The present invention is the probioticss combinations different by many plants, and probioticss produce degrading zearalenone in vivo
Enzyme, 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone is digested into innocuous substance, thus reaching the purpose eliminating toxin, this biodegradation method degraded effect
Rate is high, high specificity, effect of ejecting poison speed.And add immune polysaccharide in formula and remove free based component, play raising body
Immunity and the effect of hepatoprotective, fundamentally solve the harm that 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone brings.
Technical scheme：The bacillus subtilises of degrading zearalenone are obtained by way of biofermentation
To bacillus subtilises mycopowder, according to different feedstuff types, corresponding bacillus subtilises are compounded and forms product.
Fig. 1 is the process chart of detoxifying agent of the present invention.As illustrated, the technological process of the present invention comprises the following steps：
1st, the bacillus subtilises of degrading zearalenone in milk powder test tube will be stored in by 2% inoculum concentration, be inoculated in and hold
In the triangular flask of LB culture medium（LB culture medium prescription:Tryptone 10 g, sodium chloride 10 g, yeast extract 5 g, with steaming
Distilled water constant volume, to 1000mL, adjusts pH=7.4.After sealing, in 121 DEG C, autoclaving 20min）, and in 37 DEG C, 200r/min's
Under the conditions of culture 24h carry out seed liquor activation, then seed liquor activate is inoculated in one grade fermemtation with 2% inoculum concentration
Carry out kind of a female culture in tank（Medium component：Wheat bran 80%, rice husk 15%, bean cake 4.95%, sulfate 0.05%, material-water ratio, 1:
1.1）, the bacterium due to fermentation is aerobic bacteria, so needing during the fermentation constantly by filtrated air（Entered using steam autoclave
Row sterilizing）It is transported in fermentation tank by air compressor；
2nd, the kind mother's culture fluid completing fermenting in one grade fermemtation tank enters second order fermentation tank by sterile pipes（Medium component
With one-level fermentation tank）It is amplified cultivating, adjust medium pH=8.0 ± 0.5 value, and be constantly passed through no by air compressor
Bacterium air, adjusts fermentation temperature and is 37 ± 2 DEG C, rotating speed is 200r/min, so that probioticss is fermented and maximizes；
3rd, thalline and fermentation liquid are carried out separating by the device that is centrifuged at a high speed by the tunning in second order fermentation tank, will separate
The fermentation liquid obtaining passes through sterile pipes, is transported to exchange resin tower and is concentrated, and the fermentation liquid after concentration passes through to be spray-dried
By bacterium solution, powder is made in drying to tower rapidly, that is, obtain the mycopowder of degrading zearalenone；
According to the formula feature of different animals, the material characteristic being adopted, by 40%～60% bentonite, 20% immune polysaccharide,
10%～20% free radical scavenger, the bacillus cereuss powder of 20%～30% fermentation are compounded.Concrete preparation method：A, by above-mentioned
Ratio weighs raw material；B, immune polysaccharide, free radical scavenger are carried out mixing for the first time, obtain once mixture；C, once
The bacillus subtilis powder agent adding fermentation in mixture carries out second mixing, obtains secondary mixture；D, mixed second
Add carrier bentonite in compound, that is, obtain finished product.
The mechanism of degradation of degrading mold toxin agent is by producing bacillus subtilis enzyme, and mycotoxin is digested into non-poisonous material
Matter and reach remove toxin purpose.If it is best for proposing to make product by the enzyme of degraded toxin, but this technology
Shortcoming is that cost is too high, and the resistance of enzyme is poor.Such as：Non-refractory.
The present invention is to be produced the enzyme of degraded toxin in vivo by probioticss, toxin is digested into innocuous substance, thus reaching
Eliminate the purpose of toxin, this biodegradation method degradation efficiency is high, high specificity, effect of ejecting poison speed.And add in formula
Immune polysaccharide and the free based component of removing, play the effect improving immunity of organisms and hepatoprotective, fundamentally solve mycete
The harm that toxin brings.The present invention is to be combined the bacillus subtilises of degrading zearalenone, and bacillus subtilises enter
The internal enzyme producing degraded mycotoxin, and producing enzyme can be continued in vivo, yield of enzyme is big, and degradation efficiency is high；And hay bud
Spore bacillus is high temperature resistant, acidproof, antibiotic-resistant, strong stress resistance.So joint product advantage is larger in the form of bacillus subtilises.
Particular embodiments described above, has been carried out further to the purpose of the present invention, technical scheme and beneficial effect
Describe in detail, be should be understood that the specific embodiment that the foregoing is only the present invention, be not limited to the present invention,
All any modification, equivalent substitution and improvement within the spirit and principles in the present invention, done etc., should be included in the present invention's
Within protection domain.
Claims (5)
1. in a kind of biodegradation feedstuff the detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone it is characterised in that described detoxifying agent is by following component
Weight portion compound form：
Bentonite 40～60；
Immune polysaccharide 20；
Free radical scavenger 10～20；
Bacillus subtilis powder agent 20～30；
Wherein, described bacillus subtilis powder agent is the powder made of bacillus subtilises of degrading zearalenone.
2. in biodegradation feedstuff as claimed in claim 1 the detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone preparation technology it is characterised in that
Described preparation technology includes step：
1) the bacillus subtilises original strain ANSB01G of the degrading zearalenone that milk powder in test tube is preserved（Aerobic bacteria）
It is inoculated in by 2% inoculum concentration in the triangular flask holding LB culture medium, and in 37 DEG C, under conditions of 200r/min, culture 24h is carried out
Seed liquor activates, and the seed liquor having activated is inoculated in the one grade fermemtation tank hold culture medium with 2% inoculum concentration and carries out kind of a mother
Culture, uninterruptedly filtrated air is transported in fermentation tank by air compressor during the fermentation；
2）Female culture fluid of planting of fermentation in one grade fermemtation tank is inputted the second order fermentation tank holding LB culture medium by sterile pipes
It is amplified cultivating, the pH value adjusting culture medium in fermentation tank is 8.0 ± 0.5, and is uninterruptedly passed through no by air compressor
Bacterium air, adjusts fermentation temperature and is 37 ± 2 DEG C, rotating speed is 200r/min, so that probioticss is fermented and maximizes；
3）By being centrifuged at a high speed, thalline and fermentation liquid are carried out separating by device, pass through sterile tube by separating the fermentation liquid obtaining
Road, is transported to exchange resin tower and is concentrated, and the fermentation liquid after concentration passes through spray drying tower, and by bacterium solution, fall is made in drying rapidly
The bacillus subtilis powder agent of solution 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone；
4）The free radical scavenger of the immune polysaccharide of 20 weight portions, 10～20 weight portions is carried out mixing for the first time, obtains once
Mixture；
5）The bacillus subtilis powder agent adding 20～30 weight portion fermentations in once mixture carries out second mixing, obtains
Secondary mixture；
6）Add 40～60 parts by weight of bentonite as carrier in second mixture, that is, obtain finished product.
3. the preparation technology of detoxifying agent according to claim 2 is it is characterised in that described one grade fermemtation tank and second order fermentation
The LB culture medium prescription of tank and collocation method are:Tryptone 10g, sodium chloride 10g, yeast extract 5g, is arrived with distilled water constant volume
1000mL, adjusts pH=7.4, after sealing, in 121 DEG C, autoclaving 20min.
4. the preparation technology of detoxifying agent according to claim 3 is it is characterised in that carry out kind of a mother in described one grade fermemtation tank
The percentage by weight of medium component of culture is：Wheat bran 80%, rice husk 15%, bean cake 4.95% and sulfate 0.05＆, material-water ratio,
1:1.1.
5. the preparation technology of detoxifying agent according to claim 4 is it is characterised in that described filtrated air adopts steam sterilization
Device is sterilized.
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| CN201610946722.5A CN106472962A (en) | 2016-10-26 | 2016-10-26 | The detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its preparation technology in a kind of biodegradation feedstuff |
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| CN108034616A (en) * | 2018-01-19 | 2018-05-15 | 中国农业大学 | A kind of fermentation process of bacillus |
| CN108112777A (en) * | 2017-11-28 | 2018-06-05 | 河南智龙生物科技有限公司 | A kind of feed formula for alleviating the poisoning of growing and fattening pigs zearalenone |
| CN108456648A (en) * | 2018-03-04 | 2018-08-28 | 河南亿万中元生物技术有限公司 | A kind of method of aflatoxin B1 in degradation peanut meal |
| CN108456647A (en) * | 2018-03-04 | 2018-08-28 | 河南亿万中元生物技术有限公司 | A kind of fowl type mycotoxins in feed degradation agent and preparation method thereof |
| CN108606235A (en) * | 2018-03-04 | 2018-10-02 | 河南亿万中元生物技术有限公司 | A kind of method of zearalenone in degrading maize DDGS |
| CN109619267A (en) * | 2017-10-09 | 2019-04-16 | 北京大北农科技集团股份有限公司 | A kind of feeding mold toxin sorbent and the preparation method and application thereof |
| CN111820360A (en) * | 2019-04-17 | 2020-10-27 | 海南泓缘生物科技股份有限公司 | Biodegradation method for gibberellin ketene toxin in corn steep liquor |
| CN112063559A (en) * | 2020-09-20 | 2020-12-11 | 河南新汉博生物科技有限公司 | Zearalenone degrading strain and application thereof |
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| CN109619267A (en) * | 2017-10-09 | 2019-04-16 | 北京大北农科技集团股份有限公司 | A kind of feeding mold toxin sorbent and the preparation method and application thereof |
| CN108112777A (en) * | 2017-11-28 | 2018-06-05 | 河南智龙生物科技有限公司 | A kind of feed formula for alleviating the poisoning of growing and fattening pigs zearalenone |
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| CN108456648A (en) * | 2018-03-04 | 2018-08-28 | 河南亿万中元生物技术有限公司 | A kind of method of aflatoxin B1 in degradation peanut meal |
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| CN111820360A (en) * | 2019-04-17 | 2020-10-27 | 海南泓缘生物科技股份有限公司 | Biodegradation method for gibberellin ketene toxin in corn steep liquor |
| CN112063559A (en) * | 2020-09-20 | 2020-12-11 | 河南新汉博生物科技有限公司 | Zearalenone degrading strain and application thereof |
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Application publication date: 20170308 |