CN108606235A - A kind of method of zearalenone in degrading maize DDGS - Google Patents
A kind of method of zearalenone in degrading maize DDGS Download PDFInfo
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- CN108606235A CN108606235A CN201810177058.1A CN201810177058A CN108606235A CN 108606235 A CN108606235 A CN 108606235A CN 201810177058 A CN201810177058 A CN 201810177058A CN 108606235 A CN108606235 A CN 108606235A
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- zearalenone
- bacillus subtilis
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- seed liquor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
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- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
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Abstract
The invention is related to a kind of method of the zearalenone in degrading maize DDGS, and the bacillus subtilis once activates：The bacillus subtilis 1ml of milk tube cell preservation is taken to be inoculated in 50mlLB fluid nutrient mediums, 37 DEG C in incubator, progress seed liquor once activates for 24 hours for 200r cultures；Bacillus subtilis re-activation：It takes in step 1 activation to obtain bacillus subtilis seed liquor 2ml to be inoculated in 100mlLB fluid nutrient mediums（Inoculum concentration is 2%）, 37 DEG C in incubator；200r cultures carry out seed liquor re-activation for 24 hours；The present invention has and can play prebiotic effect to the enteron aisle of animal, achievees the effect that animal health.
Description
Technical field
The invention belongs to the zearalenone technical fields in degrading maize DDGS, and in particular to a kind of degrading maize
The method of zearalenone in DDGS.
Background technology
Corn DDGS is that object stayed by the Yu that corn obtains during producing alcohol after saccharification, fermentation, distillation remove alcohol
The product of drying process accounts for 1/3 or so of DDGS total outputs, is mainly characterized by low starch, high protein, can digest fiber with
And available phosphorus and sulfur content are high, can be widely used for husbandry sector；Yeast thalline, B family vitamin rich contents, and rich in growth
The factor is conducive to growth of animal；DDGS is more economical and can be in partial alternative animal and fowl fodder corn, dregs of beans and biphosphate
The raw material of calcium.
The pollution of mycotoxin brings serious economic loss to animal husbandry in feed and raw material, and corn DDGS is beautiful
Rice by-product, zearalenone content is relatively high, endangers animal and human health.Therefore, seek a kind of safely and effectively drop
Zearalenone in corn DDGS is solved, the nutritive value of corn DDGS can be improved, improve its utilization rate.Traditional physics and
Chemical removal method effect is unstable, and nutrient component damages are big, therefore, using in microbial solid fermentation technology degrading maize DDGS
Zearalenone, can greatly improve the utility value and economic benefit of corn DDGS, have broad application prospects.
Invention content
The purpose of the invention is to overcome the deficiencies in the prior art, and the corn in providing a kind of degrading maize DDGS is red
The method of mould ketenes.
The object of the present invention is achieved like this：A kind of method of zearalenone in degrading maize DDGS, it is described
Bacillus subtilis once activates：The bacillus subtilis 1ml of milk tube cell preservation is taken to be inoculated in 50mlLB fluid nutrient mediums, in
37 DEG C in incubator, progress seed liquor once activates for 24 hours for 200r cultures.
Bacillus subtilis re-activation：It takes in step 1 activation to obtain bacillus subtilis seed liquor 2ml to be inoculated in
In 100mlLB fluid nutrient mediums（Inoculum concentration is 2%）, 37 DEG C in incubator；200r cultures carry out seed liquor re-activation for 24 hours.
It is prepared by bacillus subtilis fermentation liquor：The bacillus subtilis seed liquor 6ml activated in step 2 is taken to be inoculated in
In 300ml fermented and cultureds（Inoculum concentration is 2%）, 37 DEG C in constant incubator, 200r culture for 24 hours.
Wherein, the preparation method of LB culture mediums is in step 1 and step 2：Tryptone 10g, sodium chloride 10g, yeast leaching
Powder 5g adjusts the .4 of pH=7,16 layers of gauze, after 8 layers of brown paper sealing, in 121 DEG C, high pressure is gone out with distilled water constant volume to 1000mL
Bacterium 20min.
Fermentation medium is grouped as by following group in step 3：Tryptone 10g, yeast extract 2g, glucose 2g, beef
Cream 3g, sodium chloride 4g, disodium hydrogen phosphate 3g, epsom salt 1.0g, 1000 ml of distilled water, pH value 7.2, after sealing, Yu Gao
121 DEG C are pressed in autoclave, and sterilize 20min.
Corn DDGS 200g is weighed in 1L large beakers, 150ml distilled water is added, goes out after mixing in 121 DEG C, high pressure
Bacterium 20min is added the bacillus subtilis fermentation liquor obtained in 10ml steps 3 after cooling, is sealed with single layer newspaper after stirring evenly
It is cultivated to 96h in 37 DEG C of incubators after mouthful（Material-water ratio is 1:0.8, inoculum concentration 5%）, and respectively in fermentation to for 24 hours, 36h,
After taking out part low temperature drying when 48h, 60h, 72h, 84h, 96h, zearalenone content therein is detected, and calculate degradation
Rate；Wherein material-water ratio is 1:0.8, inoculum concentration 5%.
Beneficial effects of the present invention：The present invention uses bacillus subtilis bacteria solid fermentation corn DDGS, safe efficient degradation
Zearalenone in corn DDGS；The solid fermentation raw materials corn DDGS that the present invention is prepared contains abundant prebiotic
Bacterium can play prebiotic effect to the enteron aisle of animal, achieve the effect that animal health.
Description of the drawings
Fig. 1 is the degradation rate collection of illustrative plates of the method for the zearalenone in a kind of degrading maize DDGS of the present invention.
Fig. 2 is the bacillus subtilis microscopy in the method for the zearalenone in a kind of degrading maize DDGS of the present invention
Collection of illustrative plates.
Specific implementation mode
Following further describes the present invention with reference to the drawings.
Embodiment 1
As shown in Figs. 1-2, a kind of method of the zearalenone in degrading maize DDGS, the bacillus subtilis once live
Change：The bacillus subtilis 1ml of milk tube cell preservation is taken to be inoculated in 50mlLB fluid nutrient mediums, 37 DEG C, 200r in incubator
Culture carries out seed liquor and once activates for 24 hours；Bacillus subtilis re-activation：Activation in step 1 is taken to obtain bacillus subtilis
Seed liquor 2ml is inoculated in 100mlLB fluid nutrient mediums（Inoculum concentration is 2%）, 37 DEG C in incubator.200r cultures carry out for 24 hours
Seed liquor re-activation.
The present invention uses bacillus subtilis bacteria solid fermentation corn DDGS, the corn in safe efficient degrading maize DDGS
Zeranol；The solid fermentation raw materials corn DDGS that the present invention is prepared contains abundant probiotics, can be to the intestines of animal
Road plays prebiotic effect, achievees the effect that animal health.
Embodiment 2
As shown in Figs. 1-2, a kind of method of the zearalenone in degrading maize DDGS, the bacillus subtilis once live
Change：The bacillus subtilis 1ml of milk tube cell preservation is taken to be inoculated in 50mlLB fluid nutrient mediums, 37 DEG C, 200r in incubator
Culture carries out seed liquor and once activates for 24 hours；Bacillus subtilis re-activation：Activation in step 1 is taken to obtain bacillus subtilis
Seed liquor 2ml is inoculated in 100mlLB fluid nutrient mediums（Inoculum concentration is 2%）, 37 DEG C in incubator；200r cultures carry out for 24 hours
Seed liquor re-activation；It is prepared by bacillus subtilis fermentation liquor：Take the bacillus subtilis seed liquor activated in step 2
6ml is inoculated in 300ml fermented and cultureds（Inoculum concentration is 2%）, 37 DEG C in constant incubator, 200r culture for 24 hours；Wherein, step
1 and step 2 in the preparation methods of LB culture mediums be：Tryptone 10g, sodium chloride 10g, yeast extract 5g, with distilled water constant volume
To 1000mL, the .4 of pH=7 are adjusted, 16 layers of gauze, after 8 layers of brown paper sealing, in 121 DEG C, high pressure sterilization 20min；It is sent out in step 3
Ferment culture medium is grouped as by following group：Tryptone 10g, yeast extract 2g, glucose 2g, beef extract 3g, sodium chloride 4g, phosphoric acid
Disodium hydrogen 3g, epsom salt 1.0g, 1000 ml of distilled water, pH value 7.2 after sealing, 121 DEG C in high-pressure sterilizing pot, are gone out
Bacterium 20min；Corn DDGS 200g is weighed in 1L large beakers, 150ml distilled water is added, goes out after mixing in 121 DEG C, high pressure
Bacterium 20min is added the bacillus subtilis fermentation liquor obtained in 10ml steps 3 after cooling, is sealed with single layer newspaper after stirring evenly
It is cultivated to 96h in 37 DEG C of incubators after mouthful（Material-water ratio is 1:0.8, inoculum concentration 5%）, and respectively in fermentation to for 24 hours, 36h,
After taking out part low temperature drying when 48h, 60h, 72h, 84h, 96h, zearalenone content therein is detected, and calculate degradation
Rate；Wherein material-water ratio is 1:0.8, inoculum concentration 5%.
Specific implementation mode is unrestricted to further explanation of the invention, is existed for those of ordinary skills
Further transformation is done to structure in the case of not departing from substantive content of the present invention, and all these transformation should all belong to institute of the present invention
Attached scope of the claims.
Claims (6)
1. a kind of method of the zearalenone in degrading maize DDGS, it is characterised in that：The bacillus subtilis is primary
Activation：The bacillus subtilis 1ml of milk tube cell preservation is taken to be inoculated in 50mlLB fluid nutrient mediums, 37 DEG C in incubator,
200r cultures carry out seed liquor and once activate for 24 hours.
2. the method for the zearalenone in a kind of degrading maize DDGS according to claim 1, it is characterised in that：It is withered
Careless bacillus re-activation：It takes in step 1 activation to obtain bacillus subtilis seed liquor 2ml and is inoculated in the training of 100mlLB liquid
It supports in base（Inoculum concentration is 2%）, 37 DEG C in incubator；200r cultures carry out seed liquor re-activation for 24 hours.
3. the method for the zearalenone in a kind of degrading maize DDGS according to claim 2, it is characterised in that：It is withered
It is prepared by careless fermentation of bacillus liquid：The bacillus subtilis seed liquor 6ml activated in step 2 is taken to be inoculated in 300ml fermentations
In culture（Inoculum concentration is 2%）, 37 DEG C in constant incubator, 200r culture for 24 hours.
4. a kind of method of the zearalenone in degrading maize DDGS according to claim 2 and 3, feature exist
In：Wherein, the preparation method of LB culture mediums is in step 1 and step 2：Tryptone 10g, sodium chloride 10g, yeast extract 5g,
With distilled water constant volume to 1000mL, the .4 of pH=7 are adjusted, 16 layers of gauze, after 8 layers of brown paper sealing, in 121 DEG C, high pressure sterilization
20min。
5. the method for the zearalenone in a kind of degrading maize DDGS according to claim 4, it is characterised in that：Step
Fermentation medium is grouped as by following group in rapid 3：Tryptone 10g, yeast extract 2g, glucose 2g, beef extract 3g, sodium chloride
4g, disodium hydrogen phosphate 3g, epsom salt 1.0g, 1000 ml of distilled water, pH value 7.2, after sealing, in high-pressure sterilizing pot
121 DEG C, sterilize 20min.
6. a kind of method of the zearalenone in degrading maize DDGS according to claim 1-5, it is characterised in that：
Corn DDGS 200g is weighed in 1L large beakers, 150ml distilled water is added, after mixing in 121 DEG C, high pressure sterilization 20min,
The bacillus subtilis fermentation liquor obtained in 10ml steps 3 is added after cooling, in 37 after being sealed with single layer newspaper after stirring evenly
Culture is to 96h in DEG C incubator（Material-water ratio is 1:0.8, inoculum concentration 5%）, and respectively in fermentation to for 24 hours, 36h, 48h, 60h,
After taking out part low temperature drying when 72h, 84h, 96h, zearalenone content therein is detected, and calculate degradation rate, wherein
Material-water ratio is 1:0.8, inoculum concentration 5%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810177058.1A CN108606235A (en) | 2018-03-04 | 2018-03-04 | A kind of method of zearalenone in degrading maize DDGS |
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| CN201810177058.1A CN108606235A (en) | 2018-03-04 | 2018-03-04 | A kind of method of zearalenone in degrading maize DDGS |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181376A (en) * | 2010-12-23 | 2011-09-14 | 中国农业大学 | Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof |
| CN103667103A (en) * | 2013-10-08 | 2014-03-26 | 暨南大学 | Bacillus subtilis for decomposing zearalenone and xylan and application thereof |
| CN103695340A (en) * | 2013-12-11 | 2014-04-02 | 中国农业大学 | Bacillus subtilis ANSB0E1 and applications thereof |
| CN106472962A (en) * | 2016-10-26 | 2017-03-08 | 北京科润生科技发展有限公司 | The detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its preparation technology in a kind of biodegradation feedstuff |
-
2018
- 2018-03-04 CN CN201810177058.1A patent/CN108606235A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181376A (en) * | 2010-12-23 | 2011-09-14 | 中国农业大学 | Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof |
| CN103667103A (en) * | 2013-10-08 | 2014-03-26 | 暨南大学 | Bacillus subtilis for decomposing zearalenone and xylan and application thereof |
| CN103695340A (en) * | 2013-12-11 | 2014-04-02 | 中国农业大学 | Bacillus subtilis ANSB0E1 and applications thereof |
| CN106472962A (en) * | 2016-10-26 | 2017-03-08 | 北京科润生科技发展有限公司 | The detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its preparation technology in a kind of biodegradation feedstuff |
Non-Patent Citations (1)
| Title |
|---|
| 周艳敬: "玉米赤霉烯酮降解酶基因表达的启动子优化研究", 《中国优秀硕士学位论文全文数据库基础科学辑》 * |
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Application publication date: 20181002 |