CN102067941A - Method for producing compound microbial preparation serving as fodder - Google Patents
Method for producing compound microbial preparation serving as fodder Download PDFInfo
- Publication number
- CN102067941A CN102067941A CN2010105810666A CN201010581066A CN102067941A CN 102067941 A CN102067941 A CN 102067941A CN 2010105810666 A CN2010105810666 A CN 2010105810666A CN 201010581066 A CN201010581066 A CN 201010581066A CN 102067941 A CN102067941 A CN 102067941A
- Authority
- CN
- China
- Prior art keywords
- temperature
- fermentation
- pressure
- bacillus subtilis
- condition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for producing a compound microbial preparation serving as fodder, which comprises the steps of microbial inoculum production, material proportioning, bacillus subtilis medium preparation, beer yeast medium preparation and lactobacillus plantarum medium preparation. According to the method, bacillus subtilis, beer yeast and lactobacillus plantarum are bred to form compound strains, so that the totality of the compound thalli is increased, and the advantages such as high strain survival rate, strong stability and the like are achieved; the compound thalli are mixed with ball-milling chaff, bran and oligoisomaltose in a proper proportion, and the optimum compounding proportion is formed for the preparation; good conditions are created for the formation of the internal environment of intestinal canals of livestock and poultry, the aerobic bacillus subtilis interacts with yeast to generate more nutrient substances, amylase, protease cellulose and the like; and meanwhile, oxygen in the intestinal canals of livestock and poultry can be consumed, excellent growth environment is created for propagation of lactic acid bacteria, intestinal canal functions of livestock and poultry are improved, the fodder conversion rate is improved, and the microecological balance of the intestinal canals of livestock and poultry is improved.
Description
Technical field
The present invention relates to a kind of preparation method of microorganism formulation, be specifically related to a kind of production method that adopts the feeding complex microorganism preparations of liquid deep layer fermenting.
Background technology
The digestive system of animal particularly has hundreds of in the intestines, tens billion of bacteriums forms intestinal flora, and it and animal health have confidential relation.Intestinal flora can also suppress the effect that pathogen propagation waits defence to infect in the various nutritional physiology effects of performance.The generation of the coliform disease of domestic animal, salmonella disease and clostruidium enteritis all with intestines in the state of bacterium substantial connection is arranged.Therefore, the balance of intestinal flora is kept normally to safeguarding that animal health is absolutely necessary.The raising and the improvement of the every index in it and the Production of Livestock and Poultry have substantial connection.
Microorganism formulation is the microbial product of one or more fungus strains in a kind of replacement or the balance animal ecology system.Say that narrowly it is a kind ofly can excite the breeding of self probiotics to increase, and resists the microbial product of harmful fungus strain growth simultaneously.
Microorganism formulation contains multiple beneficial microorganisms such as a large amount of lactic acid bacterias, saccharomycete, after entering in the livestock and poultry body as feed, can breed rapidly, drop on the one hand in the metabolin of bacterial classification and enterogenous endotoxin, suppressed the growth of other harmful flora, in host, formed normal microorganism species on the other hand, be the synthetic main vitamin of host, nutrition is provided and stops the invasion of pathogenic bacteria.
Microorganism formulation enters and resists harmful bacterium in the intestines, reduce the formation (stench is that the harmful substances such as toxicity amine, ammonia and hydrogen sulfide that produce during by harmful bacterium decomposing protein such as achalme's bacillus and amino acid cause) of stench and harmful components, make the ight soil of discharge eliminate stench, thereby alleviate the internal organs burden, make the peripheral vessel expansion, the supply of nutrition becomes smooth and easy.Simultaneously, owing to eliminated obstacle, cell proliferation is accelerated, and makes the meat, egg matter, the newborn matter that produce be improved.
Microorganism formulation product in the market mainly contains pulvis and aqua two big classes, but the general bacterial classification of powder product is single, and survival rate is low, poor stability.Aqua then transports inconvenience, and active storage life is short, easy autolyze phenomenon etc.
Summary of the invention
Purpose of the present invention is at the deficiencies in the prior art, a kind of feeding complex microorganism preparations pulvis and manufacture method thereof are provided, the composite bacteria that complex microorganism preparations adopts bacillus subtilis, saccharomycete and lactobacillus to constitute, solve defectives such as existing all kinds of microorganism formulation bacterial classifications are single, survival rate is low, poor stability, the short and easy autolyze of active storage life, increase substantially the output and the storage rate of microbial bacterial agent.
A kind of feeding complex microorganism preparations production method that provides of the present invention, this method may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, make pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis, mix with ball milling chaff, wheat bran and oligoisomaltose, after weighing, packing, produce the feeding complex microorganism preparations of powder-type, wherein, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 15～25%, wheat bran 74～84%, oligoisomaltose 0.02～1%, bacillus subtilis number 〉=2 * 10
7Cfu/g, saccharomyces cerevisiae number 〉=5 * 10
6Cfu/g and Lactobacillus plantarum number 〉=4.5 * 10
6Cfu/g.
Wherein, bacillus subtilis bacteria culture fluid of the present invention and bacillus subtilis cultural method are: glucose 5g, peptone 10g, beef extract 5g, sodium chloride 5g and water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the bacillus subtilis slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, the rotating speed when shaking bottle work is 160rmp, 37 ℃ of temperature, fermentation 24h, pH is controlled at 7.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 2.5～3m
3/ h, pH are 7.4, pressure 0.05～0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 15-20m
3/ h, pH are 7.4, and pressure 0.05～0.1MPa will collect by spray-drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again, and spray-dired formulation weight ratio is: maltodextrin 20, CaCl
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 2-3kg/cm during spray-drying
2
Brewer's yeast nutrient solution of the present invention and saccharomyces cerevisiae cultural method are: yeast extract 5g, peptone 6g, glucose 20g and distilled water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed 160rmp when shaking bottle work, 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change (temperature under 121 ℃, pressure 0.1MPa condition, sterilize 30min) the 50L seed fermentation jar of having sterilized, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 2.5～3m over to
3/ h, pH nature, pressure 0.05～0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 15～20m
3/ h, PH nature, pressure 0.05～0.1MPa.To collect by freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-33～-35 ℃, drying time 18-20h.
Lactobacillus plantarum nutrient solution of the present invention and Lactobacillus plantarum cultural method are: with peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25g, epsom salt 0.58g, glucose 20g, Tween 80 1.0 mL and distilled water 1000 mL pack in the lump and shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.2～6.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 24h, pH6.2～6.4, pressure 0.05～0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of 500L fermentation jar temperatures, fermentation 72h, pH6.2～6.4, pressure 0.05～0.1MPa.To collect by freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-33～-35 ℃, drying time 18-20h.
Moisture≤10.0% in the described feeding complex microorganism preparations, described pulvis are the composite bacteria agent capable of bacillus subtilis, brewer's yeast and Lactobacillus plantarum.
Change in order to investigate the inventive method sanitary index in process of production, the inventor counts content respectively by range estimation nasil (organoleptic indicator's determination method), GB/T 6435(aquametry to the organoleptic indicator in the product, moisture, granularity, bacterial count of bacillus subtilis, saccharomyces cerevisiae number and Lactobacillus plantarum bacterium), GB/T 5917.1(granulometry), bacillus subtilis colony counting method, brewer's yeast colony counting method and Lactobacillus plantarum colony counting method measure.
The present invention compares with existing microorganism fodder preparation technique on the market, has the following advantages:
1, the present invention adopts the liquid deep layer fermenting technology, can obviously improve live body bacterium number in the finished product, and zymotic fluid can reach 1.0 * 10
10Cfu/mL, bacterium number 〉=2 * 10 of bacillus subtilis in the product
7Cfu/g, saccharomyces cerevisiae number 〉=5 * 10
6Cfu/g, Lactobacillus plantarum number 〉=4.5 * 10
6Cfu/g.
2, the present invention has improved the sum of thalline by improving fermentation batching and raise craft condition, has solved problems such as present bacterial classification is single, the viable count storage rate is low, and bacterial classification survival rate 1 year is up to 80%.
3, the present invention is with bacillus subtilis, saccharomycete, three kinds of bacterial classifications of lactobacillus are through seed selection, and by improving fermentation batching and raise craft condition, improved the sum of composite thallus, has the bacterial classification survival rate, advantages such as stability is strong, with the ball milling chaff, wheat bran and oligoisomaltose mix with good proportioning, make it form best compositely proportional, for good condition has been created in the formation of environment in its animal and bird intestines, produce more multiple nutrient matter and amylase by aerobic bacteria bacillus subtilis and saccharomycete interaction, protease cellulose etc., can also exhaust simultaneously the oxygen in the animal and bird intestines, for good growing environment has been created in the breeding of lactic acid bacteria, improve the function of intestinal canal of livestock and poultry, improve food conversion ratio, improve livestock fowl intestinal microecology balance.
4, the inventive method is simple, and technical maturity is easy to grasp control, is convenient to promote.
The specific embodiment
Provide embodiment below so that the present invention is carried out concrete description; be pointed out that following examples can not be interpreted as limiting the scope of the invention, the person skilled in the art of this area foregoing according to the present invention still belongs to protection scope of the present invention to some nonessential improvement and the adjustment that the present invention makes.
[embodiment 1]:The production method of feeding complex microorganism preparations, it may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, make pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis, mix with ball milling chaff, wheat bran and oligoisomaltose, after weighing, packing, produce the feeding complex microorganism preparations of powder-type, in its product, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 15%, wheat bran 84%, oligoisomaltose 0.02%, wherein bacillus subtilis number 〉=2 * 10
7Cfu/g, saccharomyces cerevisiae number 〉=5 * 10
6Cfu/g and Lactobacillus plantarum number 〉=4.5 * 10
6Cfu/g.
Bacillus subtilis bacteria culture fluid and bacillus subtilis cultural method are: glucose 5.0g, peptone 10.0g, beef extract 5.0g, sodium chloride 5.0g and water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the bacillus subtilis slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed when shaking bottle work is 160rmp, 37 ℃ of temperature, fermentation 24h, pH is controlled at 7.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 2.5m
3/ h, pH are 7.4, pressure 0.05MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 15m
3/ h, pH are 7.4, and pressure 0.05MPa will collect by spray-drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again, and spray-dired formulation weight ratio is: maltodextrin 20, CaCl
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 2kg/cm during spray-drying
2
Brewer's yeast nutrient solution and saccharomyces cerevisiae cultural method are: yeast extract 5.0g, peptone 6.0g, glucose 20.0g and distilled water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed 160rmp when shaking bottle work, 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 3m
3H, pH nature, pressure 0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 20m
3/ h, the pH nature, pressure 0.1MPa will collect by freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again, cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-33 ℃, drying time 20h.
Lactobacillus plantarum nutrient solution and Lactobacillus plantarum cultural method are: with peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25g, epsom salt 0.58g, glucose 20g, Tween 80 1.0mL and distilled water 1000 mL pack in the lump and shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.2; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, and 37 ℃ of fermentation jar temperatures, fermentation 24h, pH 6.2, pressure 0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of 500L fermentation jar temperatures, fermentation 72h, pH6.4, pressure 0.1MPa, to collect by freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-35 ℃, drying time 20h.
In the inventive method in the feeding complex microorganism preparations moisture be below 10.0%.
[embodiment 2]:The production method of feeding complex microorganism preparations is characterized in that: it may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, make pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis, mix with ball milling chaff, wheat bran and oligoisomaltose, after weighing, packing, produce the feeding complex microorganism preparations of powder-type, in its product, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 25%, wheat bran 74%, oligoisomaltose 1%, wherein bacillus subtilis number 〉=2 * 10
7Cfu/g, saccharomyces cerevisiae number 〉=5 * 10
6Cfu/g and Lactobacillus plantarum number 〉=4.5 * 10
6Cfu/g.
Bacillus subtilis bacteria culture fluid and bacillus subtilis cultural method are: glucose 5.0g, peptone 10.0g, beef extract 5.0g, sodium chloride 5.0g and water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the bacillus subtilis slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed when shaking bottle work is 160rmp, 37 ℃ of temperature, fermentation 24h, pH is controlled at 7.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 3m
3/ h, pH are 7.4, pressure 0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 20m
3/ h, pH are 7.4, and pressure 0.1MPa will collect by spray-drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again, and spray-dired formulation weight ratio is: maltodextrin 20, CaCl
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 3kg/cm during spray-drying
2。
Brewer's yeast nutrient solution and saccharomyces cerevisiae cultural method are: yeast extract 5.0g, peptone 6.0g, glucose 20.0g and distilled water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed 160rmp when shaking bottle work, 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 2.5m
3/ h, pH nature, pressure 0.05MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 15m
3/ h, the pH nature, pressure 0.05MPa will collect by freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again, cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-35 ℃, drying time 18h.
Lactobacillus plantarum nutrient solution and Lactobacillus plantarum cultural method are: with peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25.0g, epsom salt 0.58g, glucose 20.0g, Tween 80 1.0mL and distilled water 1000mL pack in the lump and shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 24h, pH6.4, pressure 0.05MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 72h, pH 6.2, pressure 0.05MPa, to collect by freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-33 ℃, drying time 18h.
In the inventive method in the feeding complex microorganism preparations moisture be below 10.0%.
[embodiment 3]:The production method of feeding complex microorganism preparations, it may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, make pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis, mix with ball milling chaff, wheat bran and oligoisomaltose, after weighing, packing, produce the feeding complex microorganism preparations of powder-type, in its product, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 20%, wheat bran 79.5%, oligoisomaltose 0.5%, wherein bacillus subtilis number 〉=2 * 10
7Cfu/g, saccharomyces cerevisiae number 〉=5 * 10
6Cfu/g and Lactobacillus plantarum number 〉=4.5 * 10
6Cfu/g.
Bacillus subtilis bacteria culture fluid and bacillus subtilis cultural method are: glucose 5.0g, peptone 10.0g, beef extract 5.0g, sodium chloride 5.0g and water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed when shaking bottle work is 160rmp, 37 ℃ of temperature, fermentation 24h, pH is controlled at 7.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 2.8m
3/ h, pH are 7.4, pressure 0.08MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 18m
3/ h, pH are 7.4, and pressure 0.08MPa will collect by spray-drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again, and spray-dired formulation weight ratio is: maltodextrin 20, CaCl
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 2.5kg/cm during spray-drying
2
Brewer's yeast nutrient solution and saccharomyces cerevisiae cultural method are: yeast extract 5.0g, peptone 6.0g, glucose 20.0g and distilled water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed 160rmp when shaking bottle work, 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 2.8m
3/ h, pH nature, pressure 0.08MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 18m
3/ h, the pH nature, pressure 0.08MPa will collect by freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again, cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-34 ℃, drying time 19h.
Lactobacillus plantarum nutrient solution and Lactobacillus plantarum cultural method are: with peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25g, epsom salt 0.58g, glucose 20.0g, Tween 80 1.0mL and distilled water 1000mL pack in the lump and shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.3; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 24h, pH6.3, pressure 0.08MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of 500L fermentation jar temperatures, fermentation 72h, pH6.3, pressure 0.08MPa, to collect by freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-34 ℃, drying time 19h.
In the inventive method in the feeding complex microorganism preparations moisture be below 10.0%.
Claims (6)
1. the production method of a feeding complex microorganism preparations, it is characterized in that: it may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, make pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis, mix with ball milling chaff, wheat bran and oligoisomaltose, after weighing, packing, produce the feeding complex microorganism preparations of powder-type, wherein, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 15～25%, wheat bran 74～84%, oligoisomaltose 0.02～1%, wherein bacillus subtilis number 〉=2 * 10
7Cfu/g, saccharomyces cerevisiae number 〉=5 * 10
6Cfu/g and Lactobacillus plantarum number 〉=4.5 * 10
6Cfu/g.
2. the production method of feeding complex microorganism preparations according to claim 1, it is characterized in that: described bacillus subtilis bacteria culture fluid and bacillus subtilis cultural method are: with glucose 5g, peptone 10g, beef extract 5g, sodium chloride 5g and water 1000mL pack in the lump and shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the bacillus subtilis slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed when shaking bottle work is 160rmp, 37 ℃ of temperature, fermentation 24h, pH is controlled at 7.4; And then change 121 ℃ of temperature the 50L seed fermentation jar of sterilization 30min under the pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 2.5～3m over to
3/ h, pH are 7.4, pressure 0.05～0.1MPa; Then change temperature again over to, the 500L fermentation tank of sterilization 30min under the pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 15-20m at 121 ℃
3/ h, pH are 7.4, and pressure 0.05～0.1MPa will collect by spray-drying behind the nutrient solution universal high speed tube centrifuge enrichment thalline after fermenting again, and spray-dired formulation weight ratio is: maltodextrin 20, CaCl
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 2-3kg/cm during spray-drying
2
3. the production method of feeding complex microorganism preparations according to claim 1, it is characterized in that: described brewer's yeast nutrient solution and saccharomyces cerevisiae cultural method are: yeast extract 5g, peptone 6g, glucose 20g and distilled water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, rotating speed 160rmp when shaking bottle work, 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change 121 ℃ of temperature the 50L seed fermentation jar of sterilization 30min under the pressure 0.1MPa condition, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 2.5～3m over to
3/ h, pH nature, pressure 0.05～0.1MPa; Then change the 500L fermentation tank of sterilization 30min under the pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 15～20m again over to 121 ℃ of temperature
3/ h, the PH nature, pressure 0.05～0.1MPa will collect by freeze drying behind the nutrient solution universal high speed tube centrifuge enrichment thalline after fermenting again, cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-33～-35 ℃, drying time 18-20h.
4. the production method of feeding complex microorganism preparations according to claim 1, it is characterized in that: described Lactobacillus plantarum nutrient solution and Lactobacillus plantarum cultural method are: with peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25g, epsom salt 0.58g, glucose 20g, Tween 80 1.0 mL and distilled water 1000 mL pack in the lump and shake in the bottle, 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.2～6.4; And then change 121 ℃ of temperature the 50L seed fermentation jar of sterilization 30min under the pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 24h, pH6.2～6.4, pressure 0.05～0.1MPa over to; Then change over to again 121 ℃ of temperature, the 500L fermentation tank of sterilization 30min under the pressure 0.1MPa condition, 37 ℃ of 500L fermentation jar temperatures, fermentation 72h, pH6.2～6.4, pressure 0.05～0.1MPa will collect by freeze drying behind the nutrient solution universal high speed tube centrifuge enrichment thalline after fermenting again, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-33～-35 ℃, drying time 18-20h.
5. the production method of feeding complex microorganism preparations according to claim 1 is characterized in that: moisture≤10.0% in the described feeding complex microorganism preparations.
6. the production method of feeding complex microorganism preparations according to claim 1, it is characterized in that: the turn up 16000rmp of described high speed tube centrifuge, effectively separating thallus reaches more than 99%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105810666A CN102067941B (en) | 2010-12-09 | 2010-12-09 | Method for producing compound microbial preparation serving as fodder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105810666A CN102067941B (en) | 2010-12-09 | 2010-12-09 | Method for producing compound microbial preparation serving as fodder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102067941A true CN102067941A (en) | 2011-05-25 |
| CN102067941B CN102067941B (en) | 2012-11-28 |
Family
ID=44026849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105810666A Expired - Fee Related CN102067941B (en) | 2010-12-09 | 2010-12-09 | Method for producing compound microbial preparation serving as fodder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102067941B (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102423015A (en) * | 2011-11-29 | 2012-04-25 | 天津中裕维特生物科技有限公司 | Special synbioticmbiotic for broiler |
| CN103181478A (en) * | 2011-12-27 | 2013-07-03 | 北京资源亚太饲料科技有限公司 | Microecological preparation for poultry and its preparation method |
| CN103385362A (en) * | 2013-04-18 | 2013-11-13 | 湖北宏全生物科技有限公司 | Preparation method for biological microbial protease preparation |
| CN103518980A (en) * | 2013-05-21 | 2014-01-22 | 南京农业大学 | Preparation method for microecologics for laying hen feeds and application of microecologics |
| CN103875922A (en) * | 2014-04-09 | 2014-06-25 | 吉林大学 | Compound probiotic micro-ecological feed additive and preparation method thereof |
| CN104095148A (en) * | 2014-07-21 | 2014-10-15 | 九江礼涞生物科技有限公司 | Preparation method of products containing animal intestinal beneficial bacteria |
| CN104381686A (en) * | 2014-11-24 | 2015-03-04 | 上海创博生态工程有限公司 | Spent mushroom substrate microbial milk-cow feed and preparation method thereof |
| CN104904994A (en) * | 2015-07-14 | 2015-09-16 | 谷实农牧集团股份有限公司 | Three major types of bacteria mixed liquid fermentation, solid yeast feed additive and preparation method thereof |
| CN104988089A (en) * | 2015-06-13 | 2015-10-21 | 施瑞客（天津）生物技术有限公司 | Probiotic bacterium preparation for chicken and preparing method of preparation |
| CN106915826A (en) * | 2015-12-28 | 2017-07-04 | 北京有色金属研究总院 | A kind of repairing method of microorganism of mine tailing and mine tailing wastewater |
| CN108391758A (en) * | 2018-04-25 | 2018-08-14 | 广西康佳龙农牧集团有限公司 | Milking sow compound Synbiotics |
| CN113881600A (en) * | 2021-10-29 | 2022-01-04 | 内蒙古自治区农牧业科学院 | Liquid culture medium for simultaneously culturing two different bacilli and culture method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101371682A (en) * | 2007-08-21 | 2009-02-25 | 上海创博生态工程有限公司 | Microorganism formulation for eliminating nutrilit-resistance function and preparation method |
| CN101530165A (en) * | 2009-04-23 | 2009-09-16 | 上海谱莱生物技术有限公司 | Animal feed composite microecologic agent |
| CN101558824A (en) * | 2008-04-15 | 2009-10-21 | 北京大北农科技集团股份有限公司 | Compound micro-ecological preparation |
-
2010
- 2010-12-09 CN CN2010105810666A patent/CN102067941B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101371682A (en) * | 2007-08-21 | 2009-02-25 | 上海创博生态工程有限公司 | Microorganism formulation for eliminating nutrilit-resistance function and preparation method |
| CN101558824A (en) * | 2008-04-15 | 2009-10-21 | 北京大北农科技集团股份有限公司 | Compound micro-ecological preparation |
| CN101530165A (en) * | 2009-04-23 | 2009-09-16 | 上海谱莱生物技术有限公司 | Animal feed composite microecologic agent |
Non-Patent Citations (6)
| Title |
|---|
| 《国外畜牧学--猪与禽》 20030531 吴俊罡，张秉胜，刘吉华，秦贵江，王梅雪，张红霞，庚涛 枯草芽孢杆菌发酵培养基优化培养实验 第23卷, 第3期 * |
| 刘海燕，徐幸莲，卢士玲: "Plackett-Burman(PB)优化植物乳杆菌增殖培养基的研究", 《现代食品科技》, vol. 24, no. 12, 31 December 2008 (2008-12-31) * |
| 吴俊罡，张秉胜，刘吉华，秦贵江，王梅雪，张红霞，庚涛: "枯草芽孢杆菌发酵培养基优化培养实验", 《国外畜牧学——猪与禽》, vol. 23, no. 3, 31 May 2003 (2003-05-31) * |
| 廖湘萍, 徐功瑾, 付三乔: "利用啤酒酵母生产SOD的提取条件研究", 《酿酒科技》, no. 5, 31 December 2007 (2007-12-31) * |
| 李家鹏，张学刚，乔晓玲，牛晋阳: "响应面法优化植物乳杆菌冷冻干燥", 《食品科学》, vol. 29, no. 6, 31 December 2008 (2008-12-31) * |
| 郝林华，孙丕喜，姜振波，陈靠山，牛德庆: "枯草芽孢杆菌( Bacillus subtilis)液体发酵条件", 《上海交通大学学报(农业科学版)》, vol. 24, no. 4, 31 August 2006 (2006-08-31) * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102423015A (en) * | 2011-11-29 | 2012-04-25 | 天津中裕维特生物科技有限公司 | Special synbioticmbiotic for broiler |
| CN103181478A (en) * | 2011-12-27 | 2013-07-03 | 北京资源亚太饲料科技有限公司 | Microecological preparation for poultry and its preparation method |
| CN103385362A (en) * | 2013-04-18 | 2013-11-13 | 湖北宏全生物科技有限公司 | Preparation method for biological microbial protease preparation |
| CN103518980A (en) * | 2013-05-21 | 2014-01-22 | 南京农业大学 | Preparation method for microecologics for laying hen feeds and application of microecologics |
| CN103875922A (en) * | 2014-04-09 | 2014-06-25 | 吉林大学 | Compound probiotic micro-ecological feed additive and preparation method thereof |
| CN104095148A (en) * | 2014-07-21 | 2014-10-15 | 九江礼涞生物科技有限公司 | Preparation method of products containing animal intestinal beneficial bacteria |
| CN104381686A (en) * | 2014-11-24 | 2015-03-04 | 上海创博生态工程有限公司 | Spent mushroom substrate microbial milk-cow feed and preparation method thereof |
| CN104988089A (en) * | 2015-06-13 | 2015-10-21 | 施瑞客（天津）生物技术有限公司 | Probiotic bacterium preparation for chicken and preparing method of preparation |
| CN104904994A (en) * | 2015-07-14 | 2015-09-16 | 谷实农牧集团股份有限公司 | Three major types of bacteria mixed liquid fermentation, solid yeast feed additive and preparation method thereof |
| CN106915826A (en) * | 2015-12-28 | 2017-07-04 | 北京有色金属研究总院 | A kind of repairing method of microorganism of mine tailing and mine tailing wastewater |
| CN106915826B (en) * | 2015-12-28 | 2020-07-03 | 有研工程技术研究院有限公司 | Microbial remediation method for tailings and tailings wastewater |
| CN108391758A (en) * | 2018-04-25 | 2018-08-14 | 广西康佳龙农牧集团有限公司 | Milking sow compound Synbiotics |
| CN113881600A (en) * | 2021-10-29 | 2022-01-04 | 内蒙古自治区农牧业科学院 | Liquid culture medium for simultaneously culturing two different bacilli and culture method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102067941B (en) | 2012-11-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102067941B (en) | Method for producing compound microbial preparation serving as fodder | |
| CN111363698B (en) | Microbial inoculum for reducing mildew and mycotoxin harm of fermented feed and application | |
| CN108330080B (en) | Feed silage agent containing lactobacillus buchneri and preparation method and application thereof | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN107173522A (en) | A kind of fermented feed containing probiotics and its preparation method and application | |
| CN110226671A (en) | A kind of piglet full price fermented feed and its production method | |
| CN103966124A (en) | Compound microecological preparation and additive, as well as premix thereof | |
| CN106974063B (en) | Method for producing high-efficiency protein feed by using feed enzyme in cooperation with bacillus coagulans | |
| CN101580799A (en) | Micro-ecological preparation and application thereof | |
| CN107164269A (en) | A kind of lactobacillus paracasei, preparation and its application in pig feed | |
| CN101971920B (en) | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof | |
| CN108541805A (en) | The reuse method and fermentate of a kind of clostridium butyricum fermentation waste water and its application | |
| CN103289910A (en) | Solid fermentation production method of bacillus coagulans | |
| CN101199558A (en) | Compound probiotics for poultry and preparing method thereof | |
| CN105368755A (en) | Acid-yielding Enterococcus faecium, bacteriostatic microecological preparation and application thereof | |
| CN113549574B (en) | Bacillus coagulans and application thereof | |
| CN102321552B (en) | Clostridium butyricum used for feeding, and application thereof | |
| CN104673726A (en) | Porcine lactobacillus acidophilus freeze-drying preparation and application thereof | |
| CN109548955A (en) | A kind of additive for farm animal feed and preparation method thereof | |
| WO2017012571A1 (en) | Use of bacillus coagulans strain in increasing egg production in laying hen | |
| CN110269146A (en) | A kind of probiotics applied on feed | |
| CN110317753A (en) | A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application | |
| CN103875922B (en) | A kind of composite probiotics micro-ecological additive agent for feeding and preparation method thereof | |
| CN105166416A (en) | Compound microorganism feed additive and preparation method thereof | |
| CN108029853B (en) | Zymophyte liquid, product containing zymophyte liquid and applied to lactating sows, and preparation method and application of product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121128 Termination date: 20201209 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |