CN108391758A - Milking sow compound Synbiotics - Google Patents
Milking sow compound Synbiotics Download PDFInfo
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- CN108391758A CN108391758A CN201810378094.4A CN201810378094A CN108391758A CN 108391758 A CN108391758 A CN 108391758A CN 201810378094 A CN201810378094 A CN 201810378094A CN 108391758 A CN108391758 A CN 108391758A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a kind of milking sow compound Synbiotics, mixed bacteria liquid is added in 25~35 DEG C of physiological saline after being stirred with the rotating speed of 50~100r/min, yellow worm powder, oyster shell whiting, short chain dextrin, oligoisomaltose, fructose, hawthorn are sequentially added while stirring, after persistently stirring 15~30min, it adds citric acid and adjusts pH value to 6~7 and functional additive, compound Synbiotics is obtained after stirring evenly.Milking sow immunity can be improved the present invention provides a kind of, increase food-intake and lactation amount, immune anti-inflammatory antipyretic compound Synbiotics of competing, in addition to this, the compound Synbiotics can also promote sow postpartum recovery, accelerate normal heat, the breeding of conditioning promotion next time.
Description
Technical field
The present invention relates to pig feed additive production technical fields, more particularly to milking sow compound Synbiotics.
Background technology
Pig, which is that China is main, stores up domestic animal, and pork is also daily eaten one of the main source of meat of people.For
The disease resistance for the pig improved often adds antibiotic, however, the use of antibiotic is not in feeding process in feed
Only so that the immunity degradation of pig, while the antibiotic that can not be metabolized discharge can also remain in pork, and then enter human body, this
It is a great threat to health.As the food-safe aspect of people is increasingly paid close attention to, the meats such as pork are eaten
The control of the residual quantity of antibiotic is also increasingly stringenter in product.European Union has completely forbidden anti-using growth promotion for food animal
Raw element, while the U.S. also plans to stop using preventive antibiotics, and China is also increasingly stringent for the management and control of antibiotic, gradually
Carry out reducing and uses antibacterials.
And it is well known that the quality of agent of feed for nursing sow directly affect sow lactation amount, weanling pig and survival rate and
The weanling weight of piglet, but also the normal heat and breeding of sow after wean can be influenced.Currently, the feed of China milking sow
Existing main problem is as follows：1, palatability is bad, and feed intake is relatively low, and especially summer is especially serious；2, lactation amount is insufficient, past
It is past to cause piglet development bad；3, constipation phenomenon is serious, influences the normal of milking sow and feeds breast and feed, often to cultivation
Enterprise and cultivation user bring bigger loss, therefore, improve the feed nutrition for enhancing milking sow, replenishing and activating blood, anti-dysentery
Disease and lactation amount etc. are very crucial.
So a kind of feed addictive that can solve problem above simultaneously need to be looked for be highly desirable.
Invention content
It is an object of the invention to：In view of the above problems, provide it is a kind of can improve milking sow immunity, increase
Food-intake and lactation amount, immune anti-inflammatory antipyretic compound Synbiotics of competing, in addition to this, which can also promote sow
Postpartum recovery accelerates normal heat, the breeding of conditioning promotion next time.In order to achieve the above-mentioned object of the invention, the present invention uses
Technical solution is as follows：
Milking sow compound Synbiotics, the compound Synbiotics are prepared by the component of following mass fraction：Mixed Microbes
7~26 parts of liquid, yellow 13~22 parts of worm powder, 2~10 parts of oyster shell whiting, 15~30 parts of short chain dextrin, 5~10 parts of oligoisomaltose,
13~26 parts of fructose, 15~27 parts of citric acid, 3~10 parts of hawthorn, 7~28 parts of functional additive.
Preferably, the mixed bacteria liquid includes Enterococcus faecalis fermentation liquid, saccharomycetes to make fermentation liquid, fermentation of bacillus subtilis
Liquid, bacillus coagulans zymotic fluid, the Enterococcus faecalis fermentation liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, condensation
The weight part ratio of fermentation of bacillus liquid is 1~2:1~2:2~4:1~3.
Preferably, the Enterococcus faecalis fermentation liquid contains enterococcus faecalis 8*1010~2*1011It is a, the saccharomycetes to make fermentation liquid
Contain saccharomycete 8*1010~2*1011A, the bacillus subtilis fermentation liquor contains bacillus subtilis 1*1011~4*1011
A, the bacillus coagulans zymotic fluid contains bacillus coagulans 8*1010~2*1011It is a.
Preferably, the yellow worm powder is that drying and grinding into powder, the mesh number of the Huang worm powder are after impurities removal by yellow worm
100~300 mesh.
Preferably, the functional additive by following parts by weight at being grouped as：8~16 parts of Radix Astragali, Radix Angelicae Sinensis 3~10
Part, 5~11 parts of the stem pith of the rice-paper plant, 5~15 parts of motherwort, 5~15 parts of Ramulus Taxilli, 7~13 parts of luffa, 3~12 parts of Radix Rhapontici seu Radix Echinopsis and honeysuckle 3
~7 parts.
Preferably, the preparation method of the milking sow compound Synbiotics includes the following steps：
(1) mixed bacteria liquid is prepared
(1) Enterococcus faecalis fermentation liquid is prepared：The enterococcus faecalis activation medium of enterococcus faecalis and sterilized processing will be loaded with
35 ± 1 DEG C are warming up to the rotating speed stirring of 200~250r/min, pH value is adjusted to 30~50h of culture after 9~10, is activated
Enterococcus faecalis.Enterococcus faecalis culture solution is placed in fermentation tank through 30min125 DEG C of sterilizing postcooling to 45 DEG C, then will activation
Enterococcus faecalis be placed in fermentation tank be sealed by fermentation 3~4 days after obtain Enterococcus faecalis fermentation liquid；
(2) saccharomycetes to make fermentation liquid is prepared：By the saccharomycete activation medium for being loaded with saccharomycete and sterilized processing with 200~
The rotating speed stirring of 250r/min is maintained the temperature to 25 ± 1 DEG C, and 30~50h is cultivated between adjusting pH value to 4~5, is activated
Saccharomycete.Yeast bacteria culture fluid is placed on through 30min125 DEG C of sterilizing in fermentation tank to and is kept 25 DEG C of temperature, will be activated
Saccharomycete be placed in fermentation tank be sealed by fermentation 3~4 days after obtain saccharomycetes to make fermentation liquid；
(3) bacillus subtilis fermentation liquor is prepared：The bacillus subtilis of bacillus subtilis and sterilized processing will be loaded with
Bacterium activation medium is maintained the temperature at the rotating speed stirring of 200~250r/min to 35 ± 1 DEG C, is trained between adjusting pH value to 6~7
Support 30~50h, the bacillus subtilis activated.Bacillus subtilis bacteria culture fluid is placed on hair through 30min125 DEG C of sterilizing
In fermentation tank and 45 ± 1 DEG C of temperature is kept, the bacillus subtilis of activation is placed in fermentation tank after being sealed by fermentation 3~4 days must
To bacillus subtilis fermentation liquor；
(4) bacillus coagulans zymotic fluid is prepared：The condensation gemma bar of bacillus coagulans and sterilized processing will be loaded with
Bacterium activation medium is maintained the temperature at the rotating speed stirring of 200~250r/min to 35 ± 1 DEG C, between adjusting pH value to 9~10
Cultivate 30~50h, the bacillus coagulans activated.Bacillus coagulans culture solution is placed on through 30min125 DEG C of sterilizing
In fermentation tank and it is warming up to 45 DEG C of temperature, the bacillus coagulans of activation are placed in fermentation tank after being sealed by fermentation 3~4 days
To bacillus coagulans zymotic fluid；
(5) mixed bacteria liquid：By obtained in above-mentioned steps four kinds of zymotic fluids adjust respectively pH value to after 7~8 with 1~2:1
~2:2~4:1~3 ratio is mixed to get mixed bacteria liquid while stirring.
(2) functional additive is prepared
It is mixed after each raw material is weighed according to its mass fraction, is placed in wall-breaking machine and is crushed to 200~300 mesh, will break
After broken mixed-powder adds the water of double volume to impregnate 1.5~2h, the water etc. for being added and evaporating after 30~45min of boiling is decocted
The water of volume continues 30~45min of decoction, is evaporated to obtain bottom dry powder to be functional additive by liquid after repeating 2~3 times.
(3) compound Synbiotics is prepared
Mixed bacteria liquid is added in 25~35 DEG C of physiological saline after being stirred with the rotating speed of 50~100r/min,
Yellow worm powder, oyster shell whiting, short chain dextrin, oligoisomaltose, fructose are sequentially added while stirring, by hawthorn grind into powder
It is added, after persistently stirring 15~30min, after addition citric acid adjusting pH value to 6~7, functional additive is added, stirs evenly
Obtain compound Synbiotics.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
(1) milking sow compound Synbiotics disclosed by the invention adds the mixed bacteria liquid of four kinds of probiotics, passes through probiotics
With harmful bacteria competition oxygen, nutrition and field planting site, inhibit harmful bacteria proliferation, to change intestinal flora balance, improves enteron aisle
Health, and antibody tune is played to sow by modes such as antioxidation, the different effect of resistance, physiological activity, immunostimulations
Section, body defenses, prevention disease, treatment disease and other effects.And it the substantial amounts of these types of beneficial bacteria of intestinal tract, complements each other, is resistance to
Hydrochloric acid in gastric juice conducive to Competitive assays harmful bacteria, promotes digestion, anti-drawing to treat constipation.
(2) compound Synbiotics disclosed by the invention contains " bifidus factor " oligoisomaltose, and oligoisomaltose will not
By stomach and intestinal absorption, but it is directly entered large intestine, is preferentially utilized by the Bifidobacterium in human body, and helps its mass propagation, energy
Bifidobacterium is promoted significantly to be proliferated.Harmful bacteria cannot also utilize oligoisomaltose in enteron aisle, so as to inhibit the life of harmful bacteria
It is long, promote the Tiny ecosystem in enteron aisle to be adjusted to benign cycle, enhance immune function, prevents antibiotics to the various bad of body
Side effect improves healthcare function.
(3) compound Synbiotics of the invention also added citric acid, and citric acid, which has, to be increased feed intake, promotes digestion, carry
The high price of deed inhibits the functions such as harmful bacteria, disease-prevention health, also added functional additive, is formed with plurality of Chinese,
Rich in a variety of nutriments such as protein, amino acid, vitamin, grease, resin, carbohydrate, macroelement and trace element, and contain
There are a large amount of organic acids, alkaloid, polysaccharide, volatile oil, wax, glucoside, tannin and panimmunity active material and growth promoting activity substance,
Animal body immunity function can be enhanced.Wherein, Radix Astragali enhances immunity of organism, Chinese angelica blood supplementing and blood, and moisturize laxation adjusts immunity of organism
Function, antibacterial etc., stem pith of the rice-paper plant clearing away heat and promoting diuresis, lower breast of ventilating, motherwort can activating blood and removing stasis, excited Gilt Uterus, Ramulus Taxilli strengthening tendons are strong
Bone tonifies the liver and kidney, blood-nourishing conditioning, luffa and leakage cucurbit and cooperation lactagogue, and honeysuckle is clearing heat and detoxicating, anti-inflammatory, antipyretic, and antibacterial is anti-
Virus, enhancing are immune.These Chinese medicines are by repeatedly decocting the concentrated traditional Chinese medicine agent that evaporating water obtains, and the Chinese medicine is fully by medium-height grass
Active ingredient in medicine extracts, and is conducive to sow absorption enhancement sow postpartum recovery, increases immune and disease resistance.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention more comprehensible, preferred embodiment is enumerated below, to this hair
Bright further description.However, it is necessary to illustrate, many details listed in specification are used for the purpose of making reader to this
There are one thorough explanations for the one or more aspects of invention, can also realize the present invention's even without these specific details
These aspects.
Embodiment 1
Milking sow compound Synbiotics, the compound Synbiotics are prepared by the component of following mass fraction：Mixed Microbes
7 parts of liquid, yellow 13 parts of worm powder, 2 parts of oyster shell whiting, 15 parts of short chain dextrin, 5 parts of oligoisomaltose, 13 parts of fructose, 15 parts of citric acid,
3 parts of hawthorn, 7 parts of functional additive.
Mixed bacteria liquid includes Enterococcus faecalis fermentation liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, condensation gemma bar
Fermented liquid, the Enterococcus faecalis fermentation liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, bacillus coagulans fermentation
The weight part ratio of liquid is 1:1:2:1；Enterococcus faecalis fermentation liquid contains enterococcus faecalis 8*1010A, the saccharomycetes to make fermentation liquid contains
There is saccharomycete 8*1010A, the bacillus subtilis fermentation liquor contains bacillus subtilis 1*1011It is a, the condensation gemma bar
Fermented liquid contains bacillus coagulans 8*1010It is a；Yellow worm powder be by yellow worm after impurities removal drying and grinding into powder, yellow worm powder
Mesh number be 100 mesh；Functional additive is by following parts by weight at being grouped as：8 parts of Radix Astragali, 3 parts of Radix Angelicae Sinensis, 5 parts of the stem pith of the rice-paper plant, benefit
3 parts of 5 parts of Brittle Falsepimpernel Herb, 5 parts of Ramulus Taxilli, 7 parts of luffa, 3 parts of Radix Rhapontici seu Radix Echinopsis and honeysuckle.
The preparation method of milking sow compound Synbiotics includes the following steps：
(1) mixed bacteria liquid is prepared
(1) Enterococcus faecalis fermentation liquid is prepared：The enterococcus faecalis activation medium of enterococcus faecalis and sterilized processing will be loaded with
35 ± 1 DEG C are warming up to the rotating speed stirring of 200r/min, cultivates 30h after adjusting pH value to 9, the enterococcus faecalis activated.It will
Enterococcus faecalis culture solution is placed in fermentation tank through 30min125 DEG C of sterilizing postcooling to 45 DEG C, then the enterococcus faecalis of activation is placed in
Enterococcus faecalis fermentation liquid is obtained after being sealed by fermentation 3 days in fermentation tank；
(2) saccharomycetes to make fermentation liquid is prepared：The saccharomycete activation medium of saccharomycete and sterilized processing will be loaded with 200r/
The rotating speed stirring of min is maintained the temperature to 25 ± 1 DEG C, cultivates 30h between adjusting pH value to 4, the saccharomycete activated.By ferment
Female bacteria culture fluid is placed on through 30min125 DEG C of sterilizing in fermentation tank and keeps 25 DEG C of temperature, and the saccharomycete of activation is placed in hair
Saccharomycetes to make fermentation liquid is obtained after being sealed by fermentation 3 days in fermentation tank；
(3) bacillus subtilis fermentation liquor is prepared：The bacillus subtilis of bacillus subtilis and sterilized processing will be loaded with
Bacterium activation medium is maintained the temperature at the rotating speed stirring of 200r/min to 35 ± 1 DEG C, is cultivated 30h between adjusting pH value to 6, is obtained
To the bacillus subtilis of activation.Bacillus subtilis bacteria culture fluid is placed in fermentation tank and is kept through 30min125 DEG C of sterilizing
The bacillus subtilis of activation is placed in fermentation tank after being sealed by fermentation 3 days and obtains bacillus subtilis hair by 45 ± 1 DEG C of temperature
Zymotic fluid；
(4) bacillus coagulans zymotic fluid is prepared：The condensation gemma bar of bacillus coagulans and sterilized processing will be loaded with
Bacterium activation medium is maintained the temperature at the stirring of the rotating speed of 200r/min to 35 ± 1 DEG C, adjust pH value to culture 30 between 9~
50h, the bacillus coagulans activated.Bacillus coagulans culture solution is placed on through 30min125 DEG C of sterilizing in fermentation tank
And it is warming up to 45 DEG C of temperature, the bacillus coagulans of activation are placed in fermentation tank after being sealed by fermentation 3 days and obtain condensation gemma
Bacillus fermentation liquid；
(5) mixed bacteria liquid：By obtained in above-mentioned steps four kinds of zymotic fluids adjust respectively pH value to after 7~8 with 1:1:2:1
Ratio be mixed to get mixed bacteria liquid while stirring.
(2) functional additive is prepared
It is mixed after each raw material is weighed according to its mass fraction, is placed in wall-breaking machine and is crushed to 200 mesh, broken is mixed
Close after powder adds the water of double volume to impregnate 1.5h, decoct the isometric water of the water that is added and evaporates after boiling 30min after
It is continuous to decoct 30min, liquid is evaporated to obtain bottom dry powder to be functional additive after being repeated 3 times.
(3) compound Synbiotics is prepared
Mixed bacteria liquid is added in 25 DEG C of physiological saline after being stirred with the rotating speed of 50r/min, is stirred on one side
Yellow worm powder, oyster shell whiting, short chain dextrin, oligoisomaltose, fructose are sequentially added on one side, and hawthorn grind into powder is added, is held
After continuous stirring 15min, after addition citric acid adjusting pH value to 6, functional additive is added, is uniformly mixing to obtain compound symphysis
Element.
Embodiment 2
Milking sow compound Synbiotics is prepared by the component of following mass fraction：18 parts of mixed bacteria liquid, yellow worm powder 18
Part, 8 parts of oyster shell whiting, 22 parts of short chain dextrin, 8 parts of oligoisomaltose, 20 parts of fructose, 22 parts of citric acid, 8 parts of hawthorn, functionality
18 parts of additive.
Mixed bacteria liquid includes Enterococcus faecalis fermentation liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, condensation gemma bar
Fermented liquid, the Enterococcus faecalis fermentation liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, bacillus coagulans fermentation
The weight part ratio of liquid is 1:2:2:1；Enterococcus faecalis fermentation liquid contains enterococcus faecalis 1*1011A, saccharomycetes to make fermentation liquid contains ferment
Female bacterium 1*1011A, bacillus subtilis fermentation liquor contains bacillus subtilis 2*1011A, bacillus coagulans zymotic fluid contains
Bacillus coagulans 1*1011It is a；Yellow worm powder is that drying and grinding into powder, the mesh number of yellow worm powder are 200 after impurities removal by yellow worm
Mesh；Functional additive is by following parts by weight at being grouped as：12 parts of Radix Astragali, 8 parts of Radix Angelicae Sinensis, 7 parts of the stem pith of the rice-paper plant, 10 parts of motherwort,
5 parts of 10 parts of Ramulus Taxilli, 10 parts of luffa, 8 parts of Radix Rhapontici seu Radix Echinopsis and honeysuckle.
The preparation method of milking sow compound Synbiotics includes the following steps：
(1) mixed bacteria liquid is prepared
(1) Enterococcus faecalis fermentation liquid is prepared：The enterococcus faecalis activation medium of enterococcus faecalis and sterilized processing will be loaded with
35 ± 1 DEG C are warming up to the rotating speed stirring of 220r/min, cultivates 40h after adjusting pH value to 10, the enterococcus faecalis activated.It will
Enterococcus faecalis culture solution is placed in fermentation tank through 30min125 DEG C of sterilizing postcooling to 45 DEG C, then the enterococcus faecalis of activation is placed in
Enterococcus faecalis fermentation liquid is obtained after being sealed by fermentation 4 days in fermentation tank；
(2) saccharomycetes to make fermentation liquid is prepared：The saccharomycete activation medium of saccharomycete and sterilized processing will be loaded with 220r/
The rotating speed stirring of min is maintained the temperature to 25 ± 1 DEG C, cultivates 40h between adjusting pH value to 4, the saccharomycete activated.By ferment
Female bacteria culture fluid is placed on through 30min125 DEG C of sterilizing in fermentation tank and keeps 25 DEG C of temperature, and the saccharomycete of activation is placed in hair
Saccharomycetes to make fermentation liquid is obtained after being sealed by fermentation 4 days in fermentation tank；
(3) bacillus subtilis fermentation liquor is prepared：The bacillus subtilis of bacillus subtilis and sterilized processing will be loaded with
Bacterium activation medium is maintained the temperature at the rotating speed stirring of 220r/min to 35 ± 1 DEG C, is cultivated 40h between adjusting pH value to 7, is obtained
To the bacillus subtilis of activation.Bacillus subtilis bacteria culture fluid is placed in fermentation tank and is kept through 30min125 DEG C of sterilizing
The bacillus subtilis of activation is placed in fermentation tank after being sealed by fermentation 3 days and obtains bacillus subtilis hair by 45 ± 1 DEG C of temperature
Zymotic fluid；
(4) bacillus coagulans zymotic fluid is prepared：The condensation gemma bar of bacillus coagulans and sterilized processing will be loaded with
Bacterium activation medium is maintained the temperature at the rotating speed stirring of 220r/min to 35 ± 1 DEG C, is cultivated 40h between adjusting pH value to 9, is obtained
To the bacillus coagulans of activation.Bacillus coagulans culture solution is placed in fermentation tank and is heated up through 30min125 DEG C of sterilizing
To 45 DEG C of temperature, the bacillus coagulans of activation is placed in fermentation tank after being sealed by fermentation 4 days and obtains bacillus coagulans hair
Zymotic fluid；
(5) mixed bacteria liquid：By obtained in above-mentioned steps four kinds of zymotic fluids adjust respectively pH value to after 8 with 1:1:2:1
Ratio is mixed to get mixed bacteria liquid while stirring.
(2) functional additive is prepared
It is mixed after each raw material is weighed according to its mass fraction, is placed in wall-breaking machine and is crushed to 250 mesh, broken is mixed
Close after powder adds the water of double volume to impregnate 1.8h, decoct the isometric water of the water that is added and evaporates after boiling 40min after
It is continuous to decoct 38min, liquid is evaporated to obtain bottom dry powder to be functional additive after being repeated 3 times.
(3) compound Synbiotics is prepared
Mixed bacteria liquid is added in 30 DEG C of physiological saline after being stirred with the rotating speed of 80r/min, is stirred on one side
Yellow worm powder, oyster shell whiting, short chain dextrin, oligoisomaltose, fructose are sequentially added on one side, and hawthorn grind into powder is added, is held
After continuous stirring 22min, after addition citric acid adjusting pH value to 7, functional additive is added, is uniformly mixing to obtain compound symphysis
Element.
Embodiment 3
Milking sow compound Synbiotics, the compound Synbiotics are prepared by the component of following mass fraction：Mixed Microbes
26 parts of liquid, yellow 22 parts of worm powder, 10 parts of oyster shell whiting, 30 parts of short chain dextrin, 10 parts of oligoisomaltose, 26 parts of fructose, citric acid 27
Part, 10 parts of hawthorn, 28 parts of functional additive.
Mixed bacteria liquid includes Enterococcus faecalis fermentation liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, condensation gemma bar
Fermented liquid, the Enterococcus faecalis fermentation liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, bacillus coagulans fermentation
The weight part ratio of liquid is 2:2:4:3；Enterococcus faecalis fermentation liquid contains enterococcus faecalis 2*1011A, saccharomycetes to make fermentation liquid contains ferment
Female bacterium 2*1011A, bacillus subtilis fermentation liquor contains bacillus subtilis 4*1011A, bacillus coagulans zymotic fluid contains
Bacillus coagulans 2*1011It is a；Yellow worm powder is that drying and grinding into powder, the mesh number of yellow worm powder are 300 after impurities removal by yellow worm
Mesh；Functional additive is by following parts by weight at being grouped as：Yellow 16 parts, 10 parts of Radix Angelicae Sinensis, 11 parts of the stem pith of the rice-paper plant, 15 parts of motherwort,
7 parts of 15 parts of Ramulus Taxilli, 13 parts of luffa, 12 parts of Radix Rhapontici seu Radix Echinopsis and honeysuckle.
The preparation method of milking sow compound Synbiotics includes the following steps：
(1) mixed bacteria liquid is prepared
(1) Enterococcus faecalis fermentation liquid is prepared：The enterococcus faecalis activation medium of enterococcus faecalis and sterilized processing will be loaded with
35 ± 1 DEG C are warming up to the rotating speed stirring of 250r/min, cultivates 50h after adjusting pH value to 10, the enterococcus faecalis activated.It will
Enterococcus faecalis culture solution is placed in fermentation tank through 30min125 DEG C of sterilizing postcooling to 45 DEG C, then the enterococcus faecalis of activation is placed in
Enterococcus faecalis fermentation liquid is obtained after being sealed by fermentation 4 days in fermentation tank；
(2) saccharomycetes to make fermentation liquid is prepared：The saccharomycete activation medium of saccharomycete and sterilized processing will be loaded with 250r/
The rotating speed stirring of min is maintained the temperature to 25 ± 1 DEG C, cultivates 50h between adjusting pH value to 5, the saccharomycete activated.By ferment
Female bacteria culture fluid is placed on through 30min125 DEG C of sterilizing in fermentation tank and keeps 25 DEG C of temperature, and the saccharomycete of activation is placed in hair
Saccharomycetes to make fermentation liquid is obtained after being sealed by fermentation 4 days in fermentation tank；
(3) bacillus subtilis fermentation liquor is prepared：The bacillus subtilis of bacillus subtilis and sterilized processing will be loaded with
Bacterium activation medium is maintained the temperature at the rotating speed stirring of 250r/min to 35 ± 1 DEG C, is cultivated 50h between adjusting pH value to 7, is obtained
To the bacillus subtilis of activation.Bacillus subtilis bacteria culture fluid is placed in fermentation tank and is kept through 30min125 DEG C of sterilizing
The bacillus subtilis of activation is placed in fermentation tank after being sealed by fermentation 4 days and obtains bacillus subtilis hair by 45 ± 1 DEG C of temperature
Zymotic fluid；
(4) bacillus coagulans zymotic fluid is prepared：The condensation gemma bar of bacillus coagulans and sterilized processing will be loaded with
Bacterium activation medium is maintained the temperature at the rotating speed stirring of 250r/min to 35 ± 1 DEG C, and 50h is cultivated between adjusting pH value to 10,
The bacillus coagulans activated.Bacillus coagulans culture solution is placed in fermentation tank and is risen through 30min125 DEG C of sterilizing
The bacillus coagulans of activation are placed in fermentation tank after being sealed by fermentation 3~4 days and obtain condensation gemma bar by the temperature of temperature to 45 DEG C
Fermented liquid；
(5) mixed bacteria liquid：By obtained in above-mentioned steps four kinds of zymotic fluids adjust respectively pH value to after 8 with 2:2:4:3
Ratio is mixed to get mixed bacteria liquid while stirring.
(2) functional additive is prepared
It is mixed after each raw material is weighed according to its mass fraction, is placed in wall-breaking machine and is crushed to 300 mesh, broken is mixed
After closing the water immersion 2h that powder adds double volume, decocts the isometric water of the water for being added and evaporating after boiling 45min and continue
45min is decocted, is evaporated to obtain bottom dry powder to be functional additive by liquid after being repeated 3 times.
(3) compound Synbiotics is prepared
Mixed bacteria liquid is added in 35 DEG C of physiological saline after being stirred with the rotating speed of 100r/min, is stirred on one side
Yellow worm powder, oyster shell whiting, short chain dextrin, oligoisomaltose, fructose are sequentially added on one side, and hawthorn grind into powder is added, is held
After continuous stirring 30min, after addition citric acid adjusting pH value to 7, functional additive is added, is uniformly mixing to obtain compound symphysis
Element.
Embodiment 4
Milking sow compound Synbiotics is prepared by the component of following mass fraction：9 parts of mixed bacteria liquid, yellow worm powder 15
Part, 4 parts of oyster shell whiting, 17 parts of short chain dextrin, 6 parts of oligoisomaltose, 15 parts of fructose, 16 parts of citric acid, 4 parts of hawthorn, functionality
10 parts of additive.
Functional additive is by following parts by weight at being grouped as：9 parts of Radix Astragali, 4 parts of Radix Angelicae Sinensis, 6 parts of the stem pith of the rice-paper plant, motherwort 7
Part, 7 parts of Ramulus Taxilli, 8 parts of luffa, 4 parts of Radix Rhapontici seu Radix Echinopsis and 4 parts of honeysuckle.
Other embodiment is consistent with embodiment 2 in the present embodiment.
Embodiment 5
Milking sow compound Synbiotics is prepared by the component of following mass fraction：11 parts of mixed bacteria liquid, yellow worm powder 16
Part, 5 parts of oyster shell whiting, 19 parts of short chain dextrin, 7 parts of oligoisomaltose, 17 parts of fructose, 17 parts of citric acid, 5 parts of hawthorn, functionality
12 parts of additive.
Functional additive is by following parts by weight at being grouped as：10 parts of Radix Astragali, 5 parts of Radix Angelicae Sinensis, 7 parts of the stem pith of the rice-paper plant, motherwort 8
Part, 8 parts of Ramulus Taxilli, 9 parts of luffa, 5 parts of Radix Rhapontici seu Radix Echinopsis and 5 parts of honeysuckle.
Other embodiment is consistent with embodiment 2 in the present embodiment.
Embodiment 6
Milking sow compound Synbiotics is prepared by the component of following mass fraction：13 parts of mixed bacteria liquid, yellow worm powder 17
Part, 6 parts of oyster shell whiting, 21 parts of short chain dextrin, 8 parts of oligoisomaltose, 19 parts of fructose, 19 parts of citric acid, 6 parts of hawthorn, functionality
14 parts of additive.
Functional additive is by following parts by weight at being grouped as：11 parts of Radix Astragali, 6 parts of Radix Angelicae Sinensis, 7 parts of the stem pith of the rice-paper plant, motherwort 8
Part, 9 parts of Ramulus Taxilli, 10 parts of luffa, 6 parts of Radix Rhapontici seu Radix Echinopsis and 6 parts of honeysuckle.
Other embodiment is consistent with embodiment 2 in the present embodiment.
Embodiment 7
Milking sow compound Synbiotics is prepared by the component of following mass fraction：16 parts of mixed bacteria liquid, yellow worm powder 19
Part, 7 parts of oyster shell whiting, 23 parts of short chain dextrin, 8 parts of oligoisomaltose, 20 parts of fructose, 21 parts of citric acid, 7 parts of hawthorn, functionality
17 parts of additive.
Functional additive is by following parts by weight at being grouped as：13 parts of Radix Astragali, 8 parts of Radix Angelicae Sinensis, 8 parts of the stem pith of the rice-paper plant, motherwort 9
Part, 11 parts of Ramulus Taxilli, 11 parts of luffa, 9 parts of Radix Rhapontici seu Radix Echinopsis and 6 parts of honeysuckle.
Other embodiment is consistent with embodiment 2 in the present embodiment.
Embodiment 8
Milking sow compound Synbiotics is prepared by the component of following mass fraction：19 parts of mixed bacteria liquid, yellow worm powder 20
Part, 8 parts of oyster shell whiting, 25 parts of short chain dextrin, 8.5 parts of oligoisomaltose, 21 parts of fructose, 22 parts of citric acid, 8 parts of hawthorn, function
20 parts of additive of property.
Functional additive is by following parts by weight at being grouped as：14 parts of Radix Astragali, 8 parts of Radix Angelicae Sinensis, 9 parts of the stem pith of the rice-paper plant, motherwort
6 parts of 11 parts, 12 parts of Ramulus Taxilli, 12 parts of luffa, 10 parts of Radix Rhapontici seu Radix Echinopsis and honeysuckle.
Other embodiment is consistent with embodiment 2 in the present embodiment.
Embodiment 9
Milking sow compound Synbiotics is prepared by the component of following mass fraction：21 parts of mixed bacteria liquid, yellow worm powder 21
Part, 9 parts of oyster shell whiting, 26 parts of short chain dextrin, 9 parts of oligoisomaltose, 23 parts of fructose, 24 parts of citric acid, 9 parts of hawthorn, functionality
23 parts of additive.
Functional additive is by following parts by weight at being grouped as：15 parts of Radix Astragali, 9 parts of Radix Angelicae Sinensis, 9 parts of the stem pith of the rice-paper plant, motherwort
6 parts of 12 parts, 13 parts of Ramulus Taxilli, 12 parts of luffa, 11 parts of Radix Rhapontici seu Radix Echinopsis and honeysuckle.
Other embodiment is consistent with embodiment 2 in the present embodiment.
Embodiment 10
Milking sow compound Synbiotics is prepared by the component of following mass fraction：23 parts of mixed bacteria liquid, yellow worm powder 21
Part, 9 parts of oyster shell whiting, 27 parts of short chain dextrin, 9 parts of oligoisomaltose, 25 parts of fructose, 26 parts of citric acid, 9 parts of hawthorn, functionality
25 parts of additive.
Functional additive is by following parts by weight at being grouped as：15 parts of Radix Astragali, 10 parts of Radix Angelicae Sinensis, 10 parts of the stem pith of the rice-paper plant, benefit are female
7 parts of 13 parts of grass, 14 parts of Ramulus Taxilli, 13 parts of luffa, 11 parts of Radix Rhapontici seu Radix Echinopsis and honeysuckle.
Other embodiment is consistent with embodiment 2 in the present embodiment.
Prepared compound Synbiotics in above-described embodiment is added in sow mixed feed, additive amount 2.5%
It every kilogram, selects that kind is identical, weight is close with Breeding date and the milking sow as breeding wony, is divided into 11 groups, preceding 10
It organizes and is fed for the mixed feed for being added to the compound Synbiotics of the milking sow prepared by 1~embodiment of embodiment 10 respectively, and the 11st
It organizes and is then fed for the mixed feed for being not added with mixed feed as a control group, every group of feeding patterns all same, three times a day, in morning
Evening is each primary, and feeding capacity starts to increase by one kilogram daily since one kilogram of childbirth second day, until feeding maximum is kept to breaking
Then milk measures record sow average weight, piglet average birth weight, piglet and is averaged weaning weight, piglet survival ratio and sow
Oestrus rate, data are as shown in table 1 again in one week.
Influence of the different compound Synbiotics of table 1 to milking sow performance
As seen from the above table, the lactation fed using the mixed feed of the milking sow compound Synbiotics added with the present invention is female
Pig both can guarantee the somatogenic need of sow, and milk abundance ensures that the growth demand of piglet, the sow of control group embodiment 11 are lost
Weight and piglet daily gain be significantly lower than other embodiment, illustrate the present invention compound Synbiotics can promote milking sow recovery and
Lactation growth to piglet, and the survival rate that traditional Chinese medicine ingredients in milk improve piglet can be promoted by compound Synbiotics, and significantly
One week interior Oestrus rate again of sow is improved, the shortening sow nonpregnant phase is added to compound Synbiotics of the present invention compared with the control group
Mixed feed has significant advantage, is a big innovation of pig feed additive.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the principle of the present invention, it can also make several improvements and retouch, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (6)
1. milking sow compound Synbiotics, it is characterised in that：The compound Synbiotics by following mass fraction component prepare and
At：7~26 parts of mixed bacteria liquid, yellow 13~22 parts of worm powder, 2~10 parts of oyster shell whiting, 15~30 parts of short chain dextrin, oligoisomaltose
5~10 parts, 13~26 parts of fructose, 15~27 parts of citric acid, 3~10 parts of hawthorn, 7~28 parts of functional additive.
2. milking sow compound Synbiotics according to claim 1, it is characterised in that：The mixed bacteria liquid includes excrement intestines ball
Fermented liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, bacillus coagulans zymotic fluid, the Enterococcus faecalis fermentation
Liquid, saccharomycetes to make fermentation liquid, bacillus subtilis fermentation liquor, bacillus coagulans zymotic fluid weight part ratio be 1~2:1~2:
2~4:1~3.
3. milking sow compound Synbiotics according to claim 2, it is characterised in that：The Enterococcus faecalis fermentation liquid contains
Enterococcus faecalis 8*1010~2*1011A, the saccharomycetes to make fermentation liquid contains saccharomycete 8*1010~2*1011It is a, the withered grass gemma
Bacillus fermentation liquid contains bacillus subtilis 1*1011~4*1011A, the bacillus coagulans zymotic fluid contains condensation gemma
Bacillus 8*1010~2*1011It is a.
4. milking sow compound Synbiotics according to claim 1, it is characterised in that：The Huang worm powder is to pass through yellow worm
The mesh number of drying and grinding into powder after impurities removal, the Huang worm powder is 100~300 mesh.
5. milking sow compound Synbiotics according to claim 1, it is characterised in that：The functional additive is by following
Parts by weight at being grouped as：8~16 parts of Radix Astragali, 3~10 parts of Radix Angelicae Sinensis, 5~11 parts of the stem pith of the rice-paper plant, 5~15 parts of motherwort, Ramulus Taxilli 5
3~7 parts of~15 parts, 7~13 parts of luffa, 3~12 parts of Radix Rhapontici seu Radix Echinopsis and honeysuckle.
6. according to Claims 1 to 5 any one of them milking sow compound Synbiotics, it is characterised in that：The milking sow
The preparation method of compound Synbiotics includes the following steps：
(1) mixed bacteria liquid is prepared
(1) Enterococcus faecalis fermentation liquid is prepared：The enterococcus faecalis activation medium of enterococcus faecalis and sterilized processing will be loaded with 200
The rotating speed stirring of~250r/min is warming up to 35 ± 1 DEG C, adjusts pH value to 30~50h of culture, the excrement intestines activated after 9~10
Coccus.Enterococcus faecalis culture solution is placed in fermentation tank through 30min125 DEG C of sterilizing postcooling to 45 DEG C, then by the excrement intestines of activation
Coccus is placed in fermentation tank be sealed by fermentation 3~4 days after obtain Enterococcus faecalis fermentation liquid；
(2) saccharomycetes to make fermentation liquid is prepared：By the saccharomycete activation medium for being loaded with saccharomycete and sterilized processing with 200~
The rotating speed stirring of 250r/min is maintained the temperature to 25 ± 1 DEG C, and 30~50h is cultivated between adjusting pH value to 4~5, is activated
Saccharomycete.Yeast bacteria culture fluid is placed on through 30min125 DEG C of sterilizing in fermentation tank to and is kept 25 DEG C of temperature, will be activated
Saccharomycete be placed in fermentation tank be sealed by fermentation 3~4 days after obtain saccharomycetes to make fermentation liquid；
(3) bacillus subtilis fermentation liquor is prepared：The bacillus subtilis for being loaded with bacillus subtilis and sterilized processing is lived
Change culture medium to maintain the temperature to 35 ± 1 DEG C with the rotating speed stirring of 200~250r/min, 30 are cultivated between adjusting pH value to 6~7
~50h, the bacillus subtilis activated.Bacillus subtilis bacteria culture fluid is placed on fermentation tank through 30min125 DEG C of sterilizing
In and keep 45 ± 1 DEG C of temperature, the bacillus subtilis of activation is placed in fermentation tank after being sealed by fermentation 3~4 days obtain it is withered
Careless fermentation of bacillus liquid；
(4) bacillus coagulans zymotic fluid is prepared：The bacillus coagulans for being loaded with bacillus coagulans and sterilized processing are lived
Change culture medium to maintain the temperature to 35 ± 1 DEG C with the rotating speed stirring of 200~250r/min, be cultivated between adjusting pH value to 9~10
30~50h, the bacillus coagulans activated.Bacillus coagulans culture solution is placed on fermentation through 30min125 DEG C of sterilizing
In tank and it is warming up to 45 DEG C of temperature, the bacillus coagulans of activation is placed in fermentation tank after being sealed by fermentation 3~4 days and is coagulated
Tie fermentation of bacillus liquid；
(5) mixed bacteria liquid：By obtained in above-mentioned steps four kinds of zymotic fluids adjust respectively pH value to after 7~8 with 1~2:1~2:2
~4:1~3 ratio is mixed to get mixed bacteria liquid while stirring.
(2) functional additive is prepared
It is mixed after each raw material is weighed according to its mass fraction, is placed in wall-breaking machine and is crushed to 200~300 mesh, by what is be crushed
After mixed-powder adds the water of double volume to impregnate 1.5~2h, it is isometric to decoct the water for being added and evaporating after 30~45min of boiling
Water continue 30~45min of decoction, liquid is evaporated to obtain bottom dry powder to be functional additive after repeating 2~3 times.
(3) compound Synbiotics is prepared
Mixed bacteria liquid is added in 25~35 DEG C of physiological saline after being stirred with the rotating speed of 50~100r/min, on one side
Stirring sequentially add yellow worm powder, oyster shell whiting, short chain dextrin, oligoisomaltose, fructose, hawthorn on one side, persistently stir 15~
After 30min, after addition citric acid adjusting pH value to 6~7, functional additive is added, is uniformly mixing to obtain compound Synbiotics.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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