CN102726605A - Micro-ecological synbiotic Chinese herbal medicine feed addictive, preparation method and application thereof - Google Patents
Micro-ecological synbiotic Chinese herbal medicine feed addictive, preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a micro-ecological synbiotic Chinese herbal medicine feed addictive, comprising a micro-ecological synbiotic feed addictive and a Chinese herbal medicine additive, wherein the micro-ecological synbiotic feed addictive comprises micro-ecological probiotics and oligosaccharide, the micro-ecological probiotics is a lactic acid bacillus preparation, and the oligosaccharide is one or more of fructo-oligo saccharide, xylo oligosaccharide, isomaltose oligosaccharide and soybean oligosaccharide, the weight of the micro-ecological synbiotic feed addictive accounts for 25-75 % of that of the total weight of the micro-ecological synbiotic Chinese herbal medicine feed addictive, and the Chinese herbal medicine additive comprises the following ingredients by weight parts: malt, pericarpium citri reticulatae viride, dried orange peel, rhizoma atractylodis, medicated leaven, coke hawthorn, coke gardenia, Chinese medical stone, folium artemisiae argyi, eucommia, dichroae radix, and panax japonicus. Separate application of the additive can raise animal culture production efficiency, reduce feeding cost, enhance the immunity and anti-stress capability of animals, and reduce incidence of diseases. Combined application of the additive and the Chinese herbal medicine additive has significant synergism.
Description
Technical field
The present invention relates to the livestock rearing field, particularly microbial forage additive.
Background technology
Along with the raising day by day of fast development of national economy and living standards of the people, people have proposed requirement higher, that upgrade to breeding environment and safety, healthy animal product.China is as the first in the world animal-breeding big country; The animal product outlet is very important in national economy; But some countries such as Europe, Japan are barrier with the food-safety problem in recent years, and the part of refusing Chinese animal product gets into, and cause unnecessary loss for China's economy.This a series of problem all is due to " animal production safety " problem, has become the bottleneck of the healthy and sustainable development of the Chinese aquaculture science of restriction.
China's ecologic breeding has been obtained many progress with regulation and control in recent years, and generally believes at present that in the world probiotics will have great application prospect in the animal-breeding in future, will play bigger effect to solving animal production safety property.
Animal microecological formulation is theoretical according to animal microecology, utilizes normal microorganism member and metabolite or growth thing in the animal body, the preparation of processing through special processing technology.Have additional, adjust or keep microbial balance in the animal intestinal, reach diseases prevention, cure the disease, promote health and improve the purpose of production performance.
Probio is the main body of probiotics, and probio is active bacteria formulation again, refers to bring into play beneficial effect for improving the animal microecological balance, reaches the raising animal level of the productive forces and the general level of the health and the artificial active bacteria formulation that adds.
At the beginning of last century (1907), the famous bacteriologist plum Cheney husband of section just proposes to drink the hypothesis that sour milk can be promoted longevity.Nineteen forty-seven, Meng Hade (Mollgaard) at first finds to use the Bacillus acidi lactici piglet of feeding, and can increase the body weight of piglet effectively, and improve the physical condition of piglet.Yet the 50-70 age in 20th century has caused the research and development of probiotics to be in low tide at antibiotic research just, the gold period of producing and using, and does not receive enough attention, has just had the change of essence to change up to the mid-80.
Japan develops and utilizes one of probiotics country early in the world, to the existing 26 kinds of products of the mid-80, it is reported, reaches family surplus the enterprise existing 10 more than 20,000,000,000 yen in Japanese probiotics annual value of production so far.
Other many countries, for example all there are dissimilar probiotics products in Germany, the U.S., France, Italy, Holland, Britain, Russia and Korea S etc., and the product that has has got into China market in recent years.Like the Protexin of the PIC company of Britain, the Togocerin of Thailand's development and production adds in the feed with 0.04% ratio, can improve in-pig and lactation period sow production performance, the diarrhea rate of piglet and the death rate are obviously descended.The Prosurs Paste (mainly containing bacillus, streptococcus fecalis, Bifidobacterium etc.) that Canada produces can make the growing and fattening pigs daily gain improve 9.5%.The external at present composite bacteria agent capable that uses more.Like the Broilact of Finland, form by 32 kinds of bacterium.Kind of a microorganism was composited surplus the EM stoste of Japan belonged to 80 by 5 sections 10.
The main probiotics in domestic and international market is main with Bacillus acidi lactici and bacillus subtilis at present; Though the Bacillus acidi lactici acid producing ability is strong but thermo-labile; Dead because of heating in the feed granulating process; So additive for microbe feedstuff is selected the bacillus subtilis of the stronger high yield digestive ferment of hear resistance mostly for use, but bacillus subtilis lactic acid producing not.
Summary of the invention
The object of the present invention is to provide the mixture feed of forming with a kind of little ecological symphysis unit's feed addictive and Chinese herbal feed; The number of viable that said additive contains is high; Can significantly promote the propagation of beneficial bacterium in the young goose enteron aisle with the feed of this additive preparation; Suppress the growth of harmful bacterium, can promote the growth of livestock and poultry again, can also reduce the consumption of feed; Good stability, safely, have no side effect, noresidue, pollution-free.
For realizing above-mentioned purpose, technical scheme of the present invention is:
1, the first Chinese herbal feed additive of little ecological symphysis; Form by little ecological symphysis unit's feed addictive and Chinese herbal feed additive; The first feed addictive of little ecological symphysis is composed of the following components according to listed as parts by weight: little ecological probio 10-60 part; Oligosaccharides 40-90 part; Containing lactic acid bud pole bacterium viable count in the every gram of said little ecological symphysis unit's feed addictive is hundred million of 1-500, and said little ecological probio is the lactic acid bacillus preparation, and said oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and the soyabean oligosaccharides; The first feed addictive of said little ecological symphysis accounts for the 25-75% of the first Chinese herbal feed additive gross weight of said little ecological symphysis, and said Chinese herb feed additive is composed of the following components by weight: Fructus Hordei Germinatus 1-5 part, rascal 1-5 part, dried orange peel 1-5 part; Rhizoma atractylodis 1-5 part, Divine Comedy 1-5 part, charred FRUCTUS CRATAEGI 1-5 part; Burnt branch 1-5 part, medical stone 1-5 part, tarragon 1-5 part; Bark of eucommia 1-5 part, Changshan 1-5 part, canton fairybells root and rhizome 0.1-1 part.
Further, in the said little ecological symphysis unit feed addictive every gram to contain lactic acid bud pole bacterium viable count be hundred million of 10-300.
Further, the first feed addictive of said little ecological symphysis accounts for 45% of the first Chinese herbal feed additive gross weight of said little ecological symphysis, and said Chinese herb feed additive is composed of the following components by weight: 2 parts in Fructus Hordei Germinatus, 3 parts on rascal; 5 parts of dried orange peels, 4 parts of rhizoma atractylodis, 2 parts of Divine Comedy, 2 parts of charred FRUCTUS CRATAEGIs; 3 parts of burnt branches, 4 parts of medical stones, 3 parts of tarragons; 1 part of the bark of eucommia, 2 parts of Changshans, 0.5 part of canton fairybells root and rhizome.
The feed that contains described compositions of additives.
Further, containing mass fraction in the said feed is 0.5% said little ecological symphysis unit Chinese herbal feed additive.
Another object of the present invention is to provide the preparation method of the first feed addictive of above-mentioned little ecological symphysis, this method is simple to operate, and production cost is low, is applicable to large-scale production.
With the 4-6% inoculation that lactic acid bacillus is pressed culture volume, be 6.0-8.0 in the pH value, 37 ℃ of constant temperature leave standstill to be cultivated 24-72 hour, got the lactic acid bacillus preparation; The lactic acid bacillus culture medium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, yeast extract 0.1-0.5 part, sodium chloride 0.1-0.5 part, MnSO
4.H
2O 0.001-0.01 part, CaCl
20.001-0.01 part, MgSO
4.7H
2O 0.001-0.1 part, aqueous medium 90-99 part, sterilization; The lactic acid bacillus preparation of formula ratio and the FOS of formula ratio are fully mixed, get the first feed addictive of little ecological symphysis; Said little ecological symphysis unit's feed addictive of formula ratio and each component of said Chinese herb feed additive are fully mixed, get the first Chinese herbal feed additive of said little ecological symphysis.
Wherein, said lactic acid bacillus preparation specifically may further comprise the steps:
A enlarges kind of a daughter bacteria
Get on the inclined-plane bacterial classification inoculation and go into to be equipped with the lactic acid bacillus culture medium in vitro, 37 ℃ of constant temperature leave standstill to be cultivated 24 hours, obtained the first order seed bacterium, took out refrigerator and preserved, for use;
The preparation of B secondary seed bacterium
With the ratio of first order seed bacterium in the 4-6% of lactic acid bacillus culture volume, be inoculated in the lactic acid bacillus culture medium of sterilizing and cooling off, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium; The taking-up refrigerator is preserved, and is for use; The lactic acid bacillus culture medium is poured in the microorganism fermentation tank into sterilization and cooling;
The C fermented and cultured
With of the ratio inoculation of step B gained secondary seed bacterium in the 6-8% of the lactic acid bacillus kind culture volume of sterilizing and cooling off, stir, 37 ℃ of constant temperature leave standstill cultivates 48-72h, gets nutrient solution;
The D centrifugal dehydration
Supernatant in the centrifugal 20min of 3000r/min, is removed in step C gained nutrient solution cooling back, get bacterium mud, add the filler that is equivalent to bacterium mud weight 1-2 times and mix, place under the 40-50 ℃ of condition and dry, get the lactic acid bacillus preparation.
Wherein, the lactic acid bacillus culture medium prescription is preferably: 0.5 part of peptone, 0.3 part of beef extract, 0.1 part of yeast extract, 0.5 part in sodium chloride, MnSO
4.H
20.005 part of O, CaCl
20.00756 part, MgSO
4.7H
20.05 part of O, aqueous medium 90-99 part.
Beneficial effect of the present invention is: 1) have the following advantages with single comparing with the first feed addictive of ecological symphysis: can improve animal-breeding production efficiency, reduce feeding cost; Can strengthen immunity and the anti-stress ability of animal, reduce the incidence of disease, the few or not medication of medication helps health of human body and environmental protection; Be fit to modernization industry and produce constant product quality; 2) Chinese herbal medicine in this prescription; Build spleen, the aid digestion master's of being compound Chinese medicinal preparation to regulate the flow of vital energy; Reuse reinforcings spleen to promote digestion such as Fructus Hordei Germinatus, rascal, dried orange peel, rhizoma atractylodis, Divine Comedy, charred FRUCTUS CRATAEGI, canton fairybells root and rhizome in the side, have the Chinese medicine of the merit of strong liver kidney concurrently, tarragon, medical stone, burnt branch, Changshan, the anti-diarrhoea of the bark of eucommia, increase trace element.Fructus Hordei Germinatus has digestion-aid effect because of containing digestive ferment and Cobastab; Rascal has soothing the liver relieving stagnant Qi, the stagnant function of long-pendingization that disappear; Dried orange peel has to regulate the flow of vital energy builds spleen, eliminating dampness and eliminating phlegm effect; The rhizoma atractylodis function cures mainly drying damp and strengthening spleen, expelling wind and clearing away cold; Divine Comedy ability reinforcing spleen to promote digestion, regulating QI to expel dampness; But charred FRUCTUS CRATAEGI is food digesting stomach fortifying, the diffusing stasis of blood of promoting the circulation of qi then; Burnt branch normalizing gallbladder to cure jaundice can promote pancreatic secretion; Medical stone can prevent diarrhoea and flatulence, improve the intestines function; Tarragon has the blood of regulating the flow of vital energy by the effect of cold-dampness, can prevent and treat trusted subordinate's crymodynia, the convulsion of having loose bowels, protracted dysentery tell nosebleed; The filling liver kidney of bark of eucommia tool, strengthening the bones and muscles, many effects such as hypotensive; Changshan is vomiting phlegmatic savliva, preventing malaria then; Canton fairybells root and rhizome can be clearing heat and detoxicating, relaxes the muscles and stimulate the blood circulation.
The specific embodiment
The preparation of the first feed addictive of embodiment 1 little ecological symphysis
One, taking by weighing oligosaccharides is that each 10Kg of FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides is subsequent use;
Two, the preparation of lactic acid bacillus preparation:
Bacterial classification is the lactic acid bacillus (lactic acid bacillus) that freeze-drying is preserved; My listed as parts by weight of lactic acid bacillus culture medium is composed of the following components: 0.5 part of peptone, 0.3 part of beef extract, 0.1 part of yeast extract, 0.5 part in sodium chloride, MnSO
4Part .H
20.005 part of O, CaCl
20.00756 part, MgSO
4.7H
20.05 part of O, 99 parts of ultra-pure waters, 6.8,115 ℃ of high pressure steam sterilization 30min of pH;
1. enlarge kind of a daughter bacteria, get bacterial classification on the inclined-plane (like an oese) inoculation and go into to be equipped with the lactic acid bacillus culture medium in vitro, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the first order seed bacterium, takes out refrigerator and preserves, for use;
2. the preparation of secondary seed bacterium: with 5% the ratio of first order seed bacterium in the volume of lactic acid bacillus culture medium, be inoculated in the lactic acid bacillus culture medium, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium;
3. produce the preparation of the lactic acid bacteria culture medium of usefulness: take by weighing each composition in the composition ratio of lactic acid bacillus culture medium and be dissolved in water, pour in the microorganism fermentation tank, in 115 ℃ of steam sterilizations 30 minutes;
4. inoculation: wait that the lactic acid bacillus kind culture medium of producing usefulness is chilled to about 37 ℃, add the secondary seed bacterium down, inoculate, stir in the ratio of the 6-8% of lactic acid bacillus kind culture volume in flame control;
5. cultivate: close all breather valves, 37 ℃ of constant temperature culture 60h;
6. centrifugal dehydration: in biological fermentation tank, take out nutrient solution, refrigerator is temporary, treats that nutrient solution cooling back is centrifugal 20 minutes in 3000 rev/mins; Remove supernatant, get 2 times of chaff powder of bacterium mud adding and mix the disk of packing into; Place oven dry in 45 ℃ of air dry ovens, carry out the total count plate of lactic acid bacteria by GB 4789.35-2010 method and detect, pack; Get the lactic acid bacillus preparation, keep in Dark Place subsequent use at shady and cool dry place.
The preparation of the first feed addictive of three ecological symphysis
1) ecological symphysis unit's feed addictive prescription one (being called for short additive 1): calculate composed of the following components by weight: 40 parts in lactic acid bacillus preparation, 90 parts of FOSs, wherein, viable count is 30,000,000,000 in the ecological symphysis of the every gram unit feed addictive.
The first Chinese herbal feed additive prescription one of little ecological symphysis (being called for short composition 1): the first feed addictive prescription one of ecological symphysis accounts for 30% of compositions of additives gross weight, and surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), and said Chinese herb feed additive is composed of the following components by weight: 1 part in Fructus Hordei Germinatus, 1 part on rascal; 1 part of dried orange peel, 1 part of rhizoma atractylodis, 1 part of Divine Comedy, 5 parts of charred FRUCTUS CRATAEGIs; 5 parts of burnt branches, 5 parts of medical stones, 5 parts of tarragons; 5 parts of the barks of eucommia, 5 parts of Changshans, 0.5 part of canton fairybells root and rhizome.
2) ecological symphysis unit's feed addictive prescription two (being called for short additive 2): calculate composed of the following components by weight: 70 parts in lactic acid bacillus preparation, 30 parts of xylo-oligosaccharides, wherein, viable count is 20,000,000,000 in the ecological symphysis of the every gram of the every gram unit feed addictive.
The first Chinese herbal feed additive prescription two of little ecological symphysis (being called for short compositions 2): the first feed addictive prescription one of ecological symphysis accounts for 75% of compositions of additives gross weight, and surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 2), and said Chinese herb feed additive is composed of the following components by weight: 5 parts in Fructus Hordei Germinatus, 5 parts on rascal; 5 parts of dried orange peels, 5 parts of rhizoma atractylodis, 5 parts of Divine Comedy, 1 part of charred FRUCTUS CRATAEGI; 1 part of burnt branch, 1 part of medical stone, 1 part of tarragon; 1 part of the bark of eucommia, 1 part of Changshan, 0.5 part of canton fairybells root and rhizome.
3) ecological symphysis unit's feed addictive prescription three (being called for short additive 3): calculate composed of the following components by weight: 40 parts in lactic acid bacillus preparation, 60 parts of isomalto-oligosaccharides, wherein, viable count is 30,000,000,000 in the ecological symphysis of the every gram unit feed addictive.
The first Chinese herbal feed additive prescription three of little ecological symphysis (being called for short compositions 3): the first feed addictive prescription one of ecological symphysis accounts for 45% of compositions of additives gross weight, and surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 3), and said Chinese herb feed additive is composed of the following components by weight: 2 parts in Fructus Hordei Germinatus, 3 parts on rascal; 5 parts of dried orange peels, 4 parts of rhizoma atractylodis, 2 parts of Divine Comedy, 2 parts of charred FRUCTUS CRATAEGIs; 3 parts of burnt branches, 4 parts of medical stones, 3 parts of tarragons; 1 part of the bark of eucommia, 2 parts of Changshans, 0.5 part of canton fairybells root and rhizome.The mouthfeel of this prescription is best.
Each component in the said herbal medicine additive; See " national Chinese herbal medicine compilation " (Xie Zongwan chief editor for details; The People's Health Publisher, 1996-1052) and " Chinese medicine voluminous dictionary " (Nanjing University of Traditional Chinese Medicine writes, Shanghai science tech publishing house publication time: the 2006-3-1 second edition).
Each component of formula ratio is mixed in proportion, process granule, so that quality control, preservation, transportation and use.
The feeding effect of embodiment 2 ducklings
The test newly-hatched duckling is selected cherry paddy meat duck; Testing the same age in days of initial selection goes out strong young 240 of shell and concentrates the week of brooding to be divided into 4 groups every group 60 at random after age; If 4 repetitions, individual weight is claimed in 15 of every repetitions; After reaching homogeneity P＞0.05 between each group of variance analysis, these 4 groups examination ducks are confirmed as 4 kinds of different disposal group feeding experiment duck material, 44 days experimental periods at random.Each is organized the daily ration combination and sees table 1.
Table 1 experiment is divided into groups and the daily ration combination
Said basestocks is the conventional young goose feed of market sale, and above-mentioned percentage is weight percentage
Table 2 various combination and consumption are to the influence (g) of duckling production performance
Can find out that from table 2 in the 1-14 age in days stage, daily gain, feed intake and feedstuff-meat ratio are tested 4 groups and extremely significantly be superior to control group and other test group.
Table 3 probiotic and little ecological symphysis tuple are to the influence of Escherichia coli, lactic acid bacteria and Bifidobacterium
Can find out that from table 3 group 2 is lower than control group with coliform count in the group 4 rectum ight soil, and Bifidobacterium, Bacillus acidi lactici are counted test group and all are higher than control group, the most remarkable with group 4 especially.Show and throw something and feed probiotic especially after probiotic and prebiotics are used; Thereby the propagation that can obviously promote beneficial bacteriums such as Bifidobacterium and Bacillus acidi lactici improves the quantity of beneficial bacterium in enteron aisle, and harmful bacterium Escherichia coli is suppressed and quantity obviously reduces.The increase of beneficial bacteria of intestinal tract quantity can activate enteron aisle digestion and immunologic function, promotes digestion and the absorption of young goose, and suppresses the breeding of harmful bacterium, reduces the generation of intestines problem, thereby plays the short long effect of health care.The present invention has the following advantages with single comparing with the first feed addictive of ecological symphysis: can improve young goose breeding production efficient, reduce feeding cost; Can strengthen immunity and the anti-stress ability of young goose, reduce the incidence of disease, the few or not medication of medication helps health of human body and environmental protection; Be fit to modernization industry and produce constant product quality.
The feeding effect of embodiment 3 piglets
Selection is grown normal same parity, body weight than consistent length * big binary ablactation piglet.To supply the examination pig to be divided into 5 groups at random by body weight, sex, every group of 21 piglets are established 3 repetitions in the group, and each repeats 7 piglets, and are interior to organize, not have significant difference (P＞0.05) between organizing be principle.Experimental period is 30d, preliminary trial period 2d.Each is organized the daily ration combination and sees table 4.
Table 4 experiment is divided into groups and the daily ration combination
Said basestocks is the conventional pig starter feed of market sale; Above-mentioned percentage is weight percentage
Table 5 various combination and consumption are to the influence (g) of piglet production performance
Annotate: The data average ± standard deviation; Same line number shoulder mark
*With
*It is remarkable to be illustrated on 0.05 and 0.01 level difference.
Can find out from table 5, piglet daily gain, feed intake and feedstuff-meat ratio test 8 groups extremely significantly be superior to control group and other test group (
*P＜0.05;
*P＜0.01)
Table 6 probiotic and Synbiotics group are to the influence of escherichia coli of piglets, lactic acid bacteria and Bifidobacterium
Can find out that from table 6 group 5 is lower than control group with coliform count in the group 8 rectum ight soil, and Bifidobacterium, Bacillus acidi lactici are counted test group and all are higher than control group, the most remarkable with group 8 especially.Show and throw something and feed probiotic especially after probiotic and prebiotics are used; Thereby the propagation that can obviously promote beneficial bacteriums such as Bifidobacterium and Bacillus acidi lactici improves the quantity of beneficial bacterium in enteron aisle, and harmful bacterium Escherichia coli is suppressed and quantity obviously reduces.The increase of beneficial bacteria of intestinal tract quantity can activate enteron aisle digestion and immunologic function, promotes digestion and the absorption of young pig, and suppresses the breeding of harmful bacterium, reduces the generation of intestines problem, thereby plays the short long effect of health care.The present invention has the following advantages with single comparing with the first feed addictive of ecological symphysis: can improve young pig breeding production efficient, reduce feeding cost; Can strengthen immunity and the anti-stress ability of young pig, reduce the incidence of disease, the few or not medication of medication helps health of human body and environmental protection; Be fit to modernization industry and produce constant product quality.
In addition, behind the long-term feeding, the discovery animal is felt the preference degree of group 4 and group 8 most, and each component of said Chinese herb feed additive and weight portion are harmonious each other, and the mouthfeel of its allotment is best.
Explanation is at last; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although with reference to preferred embodiment the present invention is specified, those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention; And not breaking away from the aim and the scope of technical scheme of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (8)
1. the first Chinese herbal feed additive of little ecological symphysis; It is characterized in that; Form by little ecological symphysis unit's feed addictive and Chinese herbal feed additive; The first feed addictive of little ecological symphysis is composed of the following components according to listed as parts by weight: little ecological probio 10-60 part, and oligosaccharides 40-90 part, containing lactic acid bud pole bacterium viable count in the every gram of said little ecological symphysis unit's feed addictive is hundred million of 1-500; Said little ecological probio is the lactic acid bacillus preparation, and said oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and the soyabean oligosaccharides; The first feed addictive of said little ecological symphysis accounts for the 25-75% of the first Chinese herbal feed additive gross weight of said little ecological symphysis, and said Chinese herb feed additive is composed of the following components by weight: Fructus Hordei Germinatus 1-5 part, rascal 1-5 part, dried orange peel 1-5 part; Rhizoma atractylodis 1-5 part, Divine Comedy 1-5 part, charred FRUCTUS CRATAEGI 1-5 part; Burnt branch 1-5 part, medical stone 1-5 part, tarragon 1-5 part; Bark of eucommia 1-5 part, Changshan 1-5 part, canton fairybells root and rhizome 0.1-1 part.
2. little ecological symphysis according to claim 1 unit Chinese herbal feed additive is characterized in that, to contain lactic acid bud pole bacterium viable count be hundred million of 10-300 to every gram in the said little ecological symphysis unit feed addictive.
3. the first Chinese herbal feed additive of little ecological symphysis according to claim 1 is characterized in that: the first feed addictive of said little ecological symphysis accounts for 45% of the first Chinese herbal feed additive gross weight of said little ecological symphysis, and said Chinese herb feed additive is composed of the following components by weight: 2 parts in Fructus Hordei Germinatus, 3 parts on rascal; 5 parts of dried orange peels, 4 parts of rhizoma atractylodis, 2 parts of Divine Comedy, 2 parts of charred FRUCTUS CRATAEGIs; 3 parts of burnt branches, 4 parts of medical stones, 3 parts of tarragons; 1 part of the bark of eucommia, 2 parts of Changshans, 0.5 part of canton fairybells root and rhizome.
4. the preparation method of each described little ecological symphysis unit Chinese herbal feed additive of claim 1-3; It is characterized in that, with the 4-6% inoculation that lactic acid bacillus is pressed culture volume, be 6.0-8.0 in the pH value; 37 ℃ of constant temperature leave standstill to be cultivated 24-72 hour, got the lactic acid bacillus preparation; The lactic acid bacillus culture medium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, yeast extract 0.1-0.5 part, sodium chloride 0.1-0.5 part, MnSO
4.H
2O 0.001-0.01 part, CaCl
20.001-0.01 part, MgSO
4.7H
2O 0.001-0.1 part, aqueous medium 90-99 part, sterilization; The lactic acid bacillus preparation of formula ratio and the FOS of formula ratio are fully mixed, get the first feed addictive of little ecological symphysis; Said little ecological symphysis unit's feed addictive of formula ratio and each component of said Chinese herb feed additive are fully mixed, get the first Chinese herbal feed additive of said little ecological symphysis.
5. preparation method according to claim 4 is characterized in that, said lactic acid bacillus preparation specifically may further comprise the steps:
A enlarges kind of a daughter bacteria
Get on the inclined-plane bacterial classification inoculation and go into to be equipped with the lactic acid bacillus culture medium in vitro, 37 ℃ of constant temperature leave standstill to be cultivated 24 hours, obtained the first order seed bacterium, took out refrigerator and preserved, for use;
The preparation of B secondary seed bacterium
With the ratio of first order seed bacterium in the 4-6% of lactic acid bacillus culture volume, be inoculated in the lactic acid bacillus culture medium of sterilizing and cooling off, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium; The taking-up refrigerator is preserved, and is for use; The lactic acid bacillus culture medium is poured in the microorganism fermentation tank into sterilization and cooling;
The C fermented and cultured
With of the ratio inoculation of step B gained secondary seed bacterium in the 6-8% of the lactic acid bacillus kind culture volume of sterilizing and cooling off, stir, 37 ℃ of constant temperature leave standstill cultivates 48-72h, gets nutrient solution;
The D centrifugal dehydration
Supernatant in the centrifugal 20min of 3000r/min, is removed in step C gained nutrient solution cooling back, get bacterium mud, add the filler that is equivalent to bacterium mud weight 1-2 times and mix, place under the 40-50 ℃ of condition and dry, get the lactic acid bacillus preparation.
6. preparation method according to claim 5 is characterized in that the lactic acid bacillus culture medium prescription: 0.5 part of peptone, 0.3 part of beef extract, 0.1 part of yeast extract, 0.5 part in sodium chloride, MnSO
4.H
20.005 part of O, CaCl
20.00756 part, MgSO
4.7H
20.05 part of O, aqueous medium 90-99 part.
7. the feed that contains the first Chinese herbal feed additive of the described little ecological symphysis of claim 1.
8. feed according to claim 7 is characterized in that, contains mass fraction in the said feed and be 0.5% said little ecological symphysis unit Chinese herbal feed additive.
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| CN2012100744756A CN102726605B (en) | 2012-03-20 | 2012-03-20 | Micro-ecological synbiotic Chinese herbal medicine feed addictive, preparation method and application thereof |
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| CN2012100744756A CN102726605B (en) | 2012-03-20 | 2012-03-20 | Micro-ecological synbiotic Chinese herbal medicine feed addictive, preparation method and application thereof |
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| CN103191180A (en) * | 2013-04-12 | 2013-07-10 | 重庆市畜牧科学院 | Preparation for preventing and treating chickling salmonella enterica white diarrhea and preparation method |
| CN104522403A (en) * | 2014-12-26 | 2015-04-22 | 太仓安佑生物科技有限公司 | Special synbiotics for piglet creep feed |
| CN105146199A (en) * | 2015-09-16 | 2015-12-16 | 怀宁县森源生态养殖有限公司 | Pig feed formula |
| CN105433061A (en) * | 2015-12-23 | 2016-03-30 | 广州聚注专利研发有限公司 | Microbial fish feed additive and preparation method thereof |
| CN109287874A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For promoting the additive and its preparation method and application of ruminant cub growth and development |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103082092A (en) * | 2013-01-18 | 2013-05-08 | 刘显军 | Optimal selection method of preparation parameters of bacillus licheniformis-xylo-oligosaccharide synbiotics, preparation process and application |
| CN103191180A (en) * | 2013-04-12 | 2013-07-10 | 重庆市畜牧科学院 | Preparation for preventing and treating chickling salmonella enterica white diarrhea and preparation method |
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| CN104522403A (en) * | 2014-12-26 | 2015-04-22 | 太仓安佑生物科技有限公司 | Special synbiotics for piglet creep feed |
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| CN105433061A (en) * | 2015-12-23 | 2016-03-30 | 广州聚注专利研发有限公司 | Microbial fish feed additive and preparation method thereof |
| CN109287874A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For promoting the additive and its preparation method and application of ruminant cub growth and development |
| CN112042832A (en) * | 2020-09-28 | 2020-12-08 | 井冈山大学 | Poultry breeding microbial feed additive and preparation method and application thereof |
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Effective date of registration: 20210520 Address after: 261000 Room 101, office building, south of Yuqing street and west of Dongfang Road, high tech Zone, Weifang City, Shandong Province Patentee after: Shandong shidewaite Bioengineering Co.,Ltd. Address before: 402460, No. 770 Chang Zhou road, Rongchang County, Chongqing Patentee before: CHONGQING ACADEMY OF ANIMAL SCIENCES |