CN108029853B - Zymophyte liquid, product containing zymophyte liquid and applied to lactating sows, and preparation method and application of product - Google Patents
Zymophyte liquid, product containing zymophyte liquid and applied to lactating sows, and preparation method and application of product Download PDFInfo
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Abstract
The invention provides a zymophyte liquid, a product containing the same and applied to lactating sows, and a preparation method and application of the product, and relates to the technical field of microorganism and feed processing. The fermented feed additive provided by the invention can obviously improve the digestibility of the lactating sows to the feed. The fermented feed provided by the invention comprises the fermented feed additive, has the advantages of improving the palatability of the feed, improving the nutritional value of the feed, lowering the production cost and fully utilizing various feed raw materials, effectively improves the constipation of sows, and reduces the morbidity of sows, so that the breeding rate of piglets is improved, the intestinal health is promoted, the digestibility of the feed is improved, the pollution to the breeding environment is reduced, and the fermented feed additive has an important application prospect on the safe production of animal products.
Description
Technical Field
The invention relates to the technical field of microorganism and feed processing, in particular to a zymophyte liquid, a product containing the zymophyte liquid and applied to lactating sows, and a preparation method and application of the product.
Background
The nursing sow is the most important position in the whole pig raising production process, and the feeding management aims to obtain higher weight and survival rate of weaned piglets. If the management feeding is proper, the sow is easy to oestrous after weaning, so that the sow keeps good reproductive performance, and meanwhile, the piglets are transferred to a nursery house after weaning to be easy to feed, so that the breeding rate can be improved, and the breeding cost is reduced. If the nursing sows are not well managed and fed, the sows are too fat or too thin, the next oestrus is influenced, the nonpregnant period is prolonged, the piglets are poor in growth, the feeding management is difficult after the piglets are transferred to a nursery house, the breeding rate is low, and the whole production link is influenced. Therefore, nursing sows are burdened with a heavy task, not only supplying the nutrients they need, but also supplying the breast milk from which the newborn piglets live, grow and develop. Generally, sow lactation is associated with a number of factors: the breed, the fetal number, the litter size, the nutritional factors, the environment and the milk releasing technology, and the lactation yield of the sow is most obviously influenced by the quality and the quantity of the lactation feed. Therefore, the feed for lactating sows should contain high levels of vitamins, amino acids, high quality proteins, minerals, energy and the like in good quality to produce a large amount of high quality breast milk.
As is well known, the active fermentation type microbial ecological feed is a biological fermentation feed which takes microorganisms and complex enzyme as biological feed starter strains and converts feed raw materials into microbial mycoprotein, bioactive small peptide amino acid, microbial active probiotics, complex enzyme preparation and the like. The feed additive has the characteristics of improving the palatability of the feed, improving the nutritional value of the feed, reducing the production cost and the like, can make up for the defects of easy shortage of amino acid, low conversion rate of raw material nutrients of coarse feed and the like in the conventional feed, and achieves the effect of enhancing the digestion, absorption and utilization of the feed. At present, the fermented feed used in the breeding production has various varieties and is mostly universal for livestock and poultry, the active quantity of strains has no unified standard, the quality is uneven, the use method and the application effect are different, and the farmers are seriously puzzled.
Therefore, a method for improving the health of the lactating sows is found from the aspect of developing fermented feed so as to improve the feed utilization rate, enhance the immunity of the sows, improve the lactation capacity and the piglet breeding rate, and the method is a breeding method which not only accords with the large breeding environment, but also adapts to the national policy, and is time-saving and labor-saving.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The first purpose of the invention is to provide a zymophyte liquid, the second purpose of the invention is to provide a fermented feed additive, and the third purpose of the invention is to provide a fermented feed, so as to relieve the technical problems that the varieties of the fermented feeds used in the breeding production in the prior art are various and are mostly universal for livestock and poultry, the active quantity of strains has no unified standard, the quality is different, the using method and the application effect are different, and the technical problems of farmers are seriously troubled.
The fourth purpose of the present invention is to provide a preparation method of the fermented feed additive, so as to alleviate the technical problems of complicated preparation method and difficulty in large-scale production of the fermented feed additive in the prior art.
The invention provides a zymophyte liquid, which mainly comprises the following components in parts by weight: 1-5 parts of aspergillus oryzae, 1-5 parts of clostridium butyricum and 1-5 parts of lactobacillus delbrueckii.
Further, the zymocyte liquid mainly comprises the following components in parts by weight: 2-4 parts of aspergillus oryzae, 2-4 parts of clostridium butyricum and 2-4 parts of lactobacillus delbrueckii.
Further, the zymocyte liquid mainly comprises the following components in parts by weight: 3 parts of aspergillus oryzae, 3 parts of clostridium butyricum and 3 parts of lactobacillus delbrueckii.
Further, the concentration of aspergillus oryzae in the zymocyte liquid is 3.27-5.24 log CFU/mL; the concentration of clostridium butyricum is 4.35-5.17 log CFU/mL; the concentration of Lactobacillus delbrueckii is 4.11-5.56 log CFU/mL.
The invention also provides a fermented feed additive, which comprises the zymophyte liquid as defined in any one of claims 1-4.
Further, the fermented feed additive comprises a substrate and the fermented bacterial liquid of any one of claims 1 to 4;
wherein, the matrix mainly comprises the following components in parts by weight: 400-600 parts of corn flour, 100-150 parts of wheat bran, 40-80 parts of soybean meal, 60-90 parts of molasses, 40-100 parts of peanut meal, 40-100 parts of rapeseed meal, 10-60 parts of wheat protein powder, 10-50 parts of soybean protein concentrate, 5-10 parts of xylo-oligosaccharide and 5-10 parts of salt; 3-15 parts of zymocyte liquid.
The invention also provides a preparation method of the fermented feed additive, which comprises the following steps:
and respectively preparing the matrix and the zymophyte liquid according to the formula amount, inoculating the prepared zymophyte liquid into the matrix, uniformly mixing, and fermenting to obtain the fermented feed additive.
The invention also provides a fermented feed which comprises the fermented feed additive, a basic feed and full-fat powder.
Furthermore, in the fermented feed, the addition amount of the fermented feed additive is 2-5%, and the addition amount of the whole fat powder is 1-3%.
In addition, the invention also provides application of the fermented bacterial liquid, the fermented feed additive or the fermented feed in preparation of products for promoting intestinal health of sows, improving constipation, enhancing resistance and/or improving piglet growth rate.
The zymophyte liquid provided by the invention comprises aspergillus oryzae, clostridium butyricum and lactobacillus delbrueckii, various beneficial bacteria are combined together by compounding the excellent strains, respective functions are fully exerted, the growth and propagation of a large amount of mixed bacteria and harmful bacteria contained in a feeding system and feed raw materials can be effectively inhibited, and the good fermentation of fermented feed is ensured. The fermented feed additive provided by the invention comprises the fermented bacterial liquid provided by the invention, and the digestion and absorption rate of the lactating sow on the feed can be obviously improved through reasonable matching of the fermented bacterial liquid and the matrix. The fermented feed provided by the invention comprises the fermented feed additive, and by controlling the reasonable proportion and components of the fermented feed additive and the basic feed, the fermented feed additive has the advantages of improving the palatability of the feed, improving the nutritional value of the feed, lowering the production cost and fully utilizing various feed raw materials, and can effectively degrade anti-nutritional factors such as antigen protein and the like in the feed under the condition of not adding antibiotics, so that the effective components of the feed can be better reserved, the intestinal health of sows is promoted, the immunity and disease resistance of the sows are enhanced, the constipation of the sows is effectively improved, the morbidity of the sows is reduced, the breeding rate of piglets is increased, the intestinal health is promoted, the digestibility of the feed is improved, the pollution to the breeding environment is reduced, and the fermented feed additive has an important application prospect on the safe production of animal products.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Aiming at overcoming the technical defects of the prior product and aiming at the nutrition physiological characteristics of the lactating sow, the special fermented feed for the lactating sow is provided, and the selection, preparation and use methods of strains and fermentation substrate raw materials, the standard specification of the matched lactating sow feeding management and the like are set forth in detail. The fermented product can effectively degrade anti-nutritional factors such as antigen protein, improves the effective components and the utilization rate of the feed, is more beneficial to the early weaning of sows, and increases the weight and the survival rate of weaned piglets.
The invention provides a zymophyte liquid which mainly comprises the following components in parts by weight: 1-5 parts of aspergillus oryzae, 1-5 parts of clostridium butyricum and 1-5 parts of lactobacillus delbrueckii.
The strain is purchased from China general microbiological culture Collection center, and the preservation numbers are respectively as follows: aspergillus oryzae (CGMCC No. 3.5232), Clostridium butyricum (CGMCC number 1.336), and Lactobacillus delbrueckii (CGMCC No. 1.139).
Wherein, the aspergillus oryzae can be, but not limited to, 1 part, 2 parts, 3 parts, 4 parts or 5 parts; clostridium butyricum can be, for example, but not limited to, 1 part, 2 parts, 3 parts, 4 parts, or 5 parts; the lactobacillus delbrueckii strain may be, for example, but not limited to, 1 part, 2 parts, 3 parts, 4 parts, or 5 parts.
In a preferred embodiment, the zymocyte liquid mainly comprises the following components in parts by weight: 3 parts of aspergillus oryzae, 3 parts of clostridium butyricum and 3 parts of lactobacillus delbrueckii.
In a preferred embodiment, the concentration of Aspergillus oryzae in the fermentation broth is 3.27-5.24 log CFU/mL; the concentration of clostridium butyricum is 4.35-5.17 log CFU/mL; the concentration of Lactobacillus delbrueckii is 4.11-5.56 log CFU/mL.
In a more preferred embodiment, the concentration of Aspergillus oryzae in the fermentation broth is 4.26 log CFU/mL; the concentration of clostridium butyricum is 4.76 log CFU/mL; the concentration of Lactobacillus delbrueckii was 4.84 log CFU/mL.
The zymophyte liquid provided by the invention combines various beneficial bacteria together by compounding the excellent strains, fully exerts respective functions, can effectively inhibit the growth and reproduction of a large amount of mixed bacteria and harmful bacteria contained in a feeding system and feed raw materials, and ensures good fermentation of fermented feed.
The invention also provides a fermented feed additive which comprises the fermented bacterial liquid.
In a preferred embodiment, the fermented feed additive comprises a substrate and the above-mentioned fermented bacterial liquid;
the matrix mainly comprises the following components in parts by weight: 400-600 parts of corn flour, 100-150 parts of wheat bran, 40-80 parts of soybean meal, 60-90 parts of molasses, 40-100 parts of peanut meal, 40-100 parts of rapeseed meal, 10-60 parts of wheat protein powder, 10-50 parts of soybean protein concentrate, 5-10 parts of xylo-oligosaccharide and 5-10 parts of salt; 3-15 parts of zymophyte liquid.
Wherein, the corn flour can be, but is not limited to, 400, 450, 500, 550 or 600 parts; wheat bran may be, for example, but not limited to, 100 parts, 110 parts, 120 parts, 130 parts, 140 parts, or 150 parts; the soybean meal can be, for example, but not limited to, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, or 100 parts; molasses may be, for example, but is not limited to, 60 parts, 70 parts, 80 parts, or 90 parts; the peanut meal can be, for example, but is not limited to, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, or 100 parts; the rapeseed meal may be, for example, but not limited to, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, or 100 parts; the wheat protein powder may be, for example, but not limited to, 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, or 60 parts; the soy protein concentrate may be, for example, but is not limited to, 10 parts, 20 parts, 30 parts, 40 parts, or 50 parts; the xylo-oligosaccharide can be, for example, but not limited to, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, or 10 parts; the common salt may be, but not limited to, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts.
In a more preferred embodiment, the fermented feed additive comprises a substrate and a fermented bacterial liquid as described above;
the matrix mainly comprises the following components in parts by weight: 500 parts of corn flour, 130 parts of wheat bran, 70 parts of soybean meal, 70 parts of molasses, 70 parts of peanut meal, 70 parts of rapeseed meal, 40 parts of wheat protein powder, 30 parts of soybean protein concentrate, 8 parts of xylo-oligosaccharide and 8 parts of salt; the zymophyte liquid is 9 parts by weight.
The invention also provides a preparation method of the fermented feed additive, which comprises the following steps:
respectively preparing a substrate and a zymophyte liquid according to the formula amount, inoculating the prepared zymophyte liquid into the substrate, uniformly mixing, and fermenting to obtain the fermented feed additive.
Wherein, the fermentation method comprises the following steps: inoculating the zymophyte liquid into the matrix, and uniformly mixing, wherein the inoculation amount of the zymophyte liquid in the matrix is 5-8% (v/w); the material-water ratio is 1: 1-1: 0.4 according to the weight-volume ratio; the temperature is 35-40 ℃; the fermentation time is 90-120 h.
The fermented feed additive provided by the invention is simple in preparation process, simple and convenient in operation because fermentation process and fermentation process parameters are tested and optimized, and is easy to operate by various farmers and feed production enterprises.
The invention also provides a fermented feed which comprises the fermented feed additive, a basic feed and full-fat powder.
Wherein the basic feed comprises the following components in percentage by total weight: 0.15% of multi-mineral for sows, 0.05% of breast-feeding multi-vitamin, 0.1% of 98 lysine, 0.05% of antioxidant, 0.05% of mildew preventive, 0.01% of sweetener, 10000IU0.02% of phytase and 0.1% of sodium bicarbonate5%，MgSO40.15 percent, 0.02 percent of VE, 0.002 percent of 1 percent of chromium picolinate, 0.015 percent of yeast selenium and 0.233 percent of zeolite powder.
The whole powder comprises the following components: crude fat is more than or equal to 82 percent, calcium is more than or equal to 6.5 percent, and water content is less than or equal to 10 percent; color: light yellow, total energy 33.6 MJ/kg; the fatty acid component ratio is as follows: 10.4% of palmitic acid, 3.6% of stearic acid, 19.9% of oleic acid, 53.3% of linoleic acid and 7.3% of linolenic acid.
In a preferred embodiment, the fermented feed additive is added in an amount of 2-5% and the whole fat powder is added in an amount of 1-3% in the fermented feed.
Wherein, the addition amount of the fermented feed additive can be, but is not limited to, 2%, 3%, 4% or 5%; the amount of whole fat powder added may be, for example, but is not limited to, 1%, 2% or 3%.
In the invention, the addition amount of the fermented feed additive refers to the percentage content of the mass of the fermented feed additive in the mass of the fermented feed.
In addition, the invention also provides application of the fermented bacterial liquid, the fermented feed additive or the fermented feed in preparation of products for promoting intestinal health of sows, improving constipation, enhancing resistance and/or improving piglet growth rate.
The clostridium butyricum and lactobacillus delbrueckii in the zymocyte liquid are excellent strains suitable for solid-state fermented feed, can effectively inhibit the growth and reproduction of a large amount of mixed bacteria and harmful bacteria contained in a feeding system and feed raw materials, and ensure the good fermentation of the fermented solid-state feed. Aspergillus oryzae can produce a large amount of protease, amylase, glucoamylase, cellulase, phytase, etc. by using raw materials, and can degrade amylose and amylopectin in the raw materials into dextrin and various low-molecular saccharides, such as maltose, glucose, etc.; the indigestible macromolecular protein is degraded into peptone, polypeptide and various amino acids, and the substances which are difficult to absorb, such as crude fiber, phytic acid and the like in the raw materials can be degraded, so that the nutritional value is improved. Most of harmful microorganisms in the intestinal tract are aerobic microorganisms, clostridium butyricum and lactobacillus delbrueckii used in the invention are anaerobic microorganisms, and the aspergillus oryzae oxygen-consuming microorganisms can quickly exhaust oxygen in the intestinal tract, thereby creating favorable conditions for the growth of beneficial microorganisms. Xylo-oligosaccharide added in the substrate can not be utilized by harmful microorganisms, but can provide nutrition for beneficial bacteria, promote proliferation and simultaneously has the function of enhancing the immune function of organisms.
Therefore, the feed disclosed by the invention combines various beneficial bacteria together, fully exerts respective functions, can obviously improve the digestibility of the lactating sow to the feed, simultaneously promotes the intestinal health of the sow, enhances the immunity and disease resistance of the sow, effectively improves the constipation of the sow, reduces the morbidity of the sow and further improves the breeding rate of piglets. The preparation process of the fermented feed is simple, the fermentation process and the fermentation process parameters are tested and optimized by the applicant, and the fermented feed is simple and convenient and is easy to operate by various farmers and feed production enterprises. The preparation process of the composite bacteria antibiotic-free fermented feed and the final product of the invention have no antibiotic, are green and safe and are environment-friendly.
In order to facilitate a further understanding of the present invention, the technical solutions of the present invention will now be described in detail with reference to the preferred embodiments.
Preparation of zymocyte liquid
The preparation method of the clostridium butyricum seed liquid comprises the following steps: inoculating the activated clostridium strain into 200 mL of a preset RCM (clostridium multiplication medium component: 3 g/L of yeast extract, 10 g/L of beef extract, 10 g/L of tryptone, 5 g/L of glucose, 1 g/L of soluble starch, 5 g/L of sodium chloride, 3 g/L of sodium acetate trihydrate, 0.15 g/L of cysteine hydrochloride and 1L of water) medium, adjusting the pH value to be 7.3, and culturing for 18 h in a constant temperature shaking table at 37 ℃, wherein the rotating speed of the shaking table is 220 rpm, thus obtaining the spare clostridium butyricum seed liquid.
The preparation method of the lactobacillus delbrueckii seed liquid comprises the following steps: inoculating the activated Bifidobacterium iniae strain to a culture medium (20 g/L glucose, 10 g/L peptone, 10 g/L beef extract, 5 g/L yeast extract, 2 g/L dipotassium phosphate, 5 g/L anhydrous sodium acetate, 2 g/L triammonium citrate, 0.58 g/L magnesium sulfate heptahydrate, 0.25 g/L manganese sulfate tetrahydrate, 801 mL Tween and 1000 mL distilled water) filled with MSR, adjusting the pH value to 6.2, culturing for 24 h in a constant temperature shaking table at 37 ℃, and adjusting the rotating speed of the shaking table to 220 rpm to obtain the standby Lactobacillus delbrueckii seed liquid.
The preparation method of the aspergillus oryzae seed liquid comprises the following steps: inoculating the activated aspergillus oryzae strain to a culture medium (bean cake extract: 100 g bean cake, water 500 mL, soaking for 4 h, boiling for 3-4 h, natural filtering with gauze, collecting the solution, adjusting to 5 Baume degree, 20 g/L soluble starch, 1 g/L potassium dihydrogen phosphate, 0.5 g/L magnesium sulfate, 0.5 g/L ammonium sulfate and 20 g/L agar) with natural pH, and culturing in a constant temperature shaking table at 37 ℃ for 20 h, wherein the rotation speed of the shaking table is 200 rpm, thus obtaining the spare aspergillus oryzae seed solution.
And (3) mixing the prepared seed liquid by 3 parts of aspergillus oryzae seed liquid, 3 parts of clostridium butyricum seed liquid and 3 parts of lactobacillus delbrueckii seed liquid in parts by weight, and uniformly stirring to obtain a zymogen liquid. Wherein the concentration of Aspergillus oryzae is 3.27-5.24 log CFU/mL; the concentration of clostridium butyricum is 4.35-5.17 log CFU/mL; the concentration of Lactobacillus delbrueckii is 4.11-5.56 log CFU/mL.
Fermented feed additive and preparation of fermented feed
Example 1
1) Preparing a zymophyte liquid: taking the prepared microbial seed liquid, and uniformly mixing according to 30 ml of clostridium butyricum liquid, 30 ml of lactobacillus delbrueckii liquid and 30 ml of aspergillus oryzae liquid, and totaling to 90 ml.
2) The matrix composition is as follows: 500 g of corn flour, 130 g of bran, 70 g of molasses, 70 g of bean pulp, 70 g of rapeseed meal, 70 g of peanut meal, 40 g of wheat protein powder, 30 g of soybean protein concentrate, 8g of xylo-oligosaccharide and 8g of salt, and all the raw materials are mixed and stirred uniformly.
3) Inoculating 90 ml of the zymocyte liquid into a matrix, adding 600 ml of water, and uniformly mixing; placing in a sterile closed fermentation tank, and fermenting at 37 deg.C; the fermentation time is 100 h. Determining the concentration of Aspergillus oryzae in the fermentation feed to be 4.26 log CFU/mL; the concentration of clostridium butyricum is 4.76 log CFU/mL; the concentration of the lactobacillus delbrueckii is 4.84 log CFU/mL, and the content of soluble protein reaches 13.24%.
4) After fermentation is finished, the fermented feed is dried and crushed in the air in a natural state, 5% of the fermented feed is added into a preset sow feed to be used as a feed raw material, complete feed is prepared, and the fermented feed is uniformly mixed to obtain the fermented feed special for the lactating sows.
Example 2
1) Preparing a zymophyte liquid: taking the prepared microbial seed liquid, and uniformly mixing according to 10 ml of clostridium butyricum liquid, 50 ml of lactobacillus delbrueckii liquid and 10 ml of aspergillus oryzae liquid, and accounting for 70 ml.
2) The matrix composition is as follows: 400 g of corn flour, 150 g of bran, 90 g of molasses, 40 g of soybean meal, 40 g of rapeseed meal, 100 g of peanut meal, 10g of wheat protein powder, 50 g of soybean protein concentrate, 5g of xylo-oligosaccharide and 10g of salt, and all the raw materials are mixed and stirred uniformly.
3) Inoculating 70 ml of the zymocyte liquid into a matrix, adding 600 ml of water, and uniformly mixing; placing in a sterile closed fermentation tank, and fermenting at 37 deg.C; the fermentation time is 100 h.
4) After fermentation is finished, the fermented feed is dried and crushed in the air in a natural state, 5% of the fermented feed is added into a preset sow feed to be used as a feed raw material, complete feed is prepared, and the fermented feed is uniformly mixed to obtain the fermented feed special for the lactating sows.
Example 3
1) Preparing a zymophyte liquid: taking the prepared microbial seed liquid, and uniformly mixing according to the total volume of 50 ml of clostridium butyricum liquid, 10 ml of lactobacillus delbrueckii liquid and 50 ml of aspergillus oryzae liquid, and 110 ml.
2) The matrix composition is as follows: 600 g of corn flour, 100 g of bran, 60 g of molasses, 80 g of bean pulp, 100 g of rapeseed meal, 40 g of peanut meal, 60 g of wheat protein powder, 10g of soybean protein concentrate, 10g of xylo-oligosaccharide and 5g of salt, and the raw materials are mixed and stirred uniformly.
3) Inoculating 110 ml of the zymocyte liquid into a matrix, adding 600 ml of water, and uniformly mixing; placing in a sterile closed fermentation tank, and fermenting at 37 deg.C; the fermentation time is 100 h.
4) After fermentation is finished, the fermented feed is dried and crushed in the air in a natural state, 5% of the fermented feed is added into a preset sow feed to be used as a feed raw material, complete feed is prepared, and the fermented feed is uniformly mixed to obtain the fermented feed special for the lactating sows.
Comparative example 1
1) Preparing a zymophyte liquid: and (3) taking the prepared microbial seed liquid, and uniformly mixing according to 30 ml of lactobacillus delbrueckii bacterial liquid and 30 ml of aspergillus oryzae bacterial liquid, wherein the total amount is 60 ml.
2) The matrix composition is as follows: 500 g of corn flour, 130 g of bran, 70 g of molasses, 70 g of bean pulp, 70 g of rapeseed meal, 70 g of peanut meal, 40 g of wheat protein powder, 30 g of soybean protein concentrate, 8g of xylo-oligosaccharide and 8g of salt, and all the raw materials are mixed and stirred uniformly.
3) Inoculating 60 ml of the zymocyte liquid into a matrix, adding 600 ml of water, and uniformly mixing; placing in a sterile closed fermentation tank, and fermenting at 37 deg.C; the fermentation time is 100 h.
4) After fermentation is finished, the fermented feed is dried and crushed in the air in a natural state, 5% of the fermented feed is added into a preset sow feed to be used as a feed raw material, complete feed is prepared, and the fermented feed is uniformly mixed to obtain the fermented feed special for the lactating sows.
Comparative example 2
1) Preparing a zymophyte liquid: and (3) uniformly mixing the prepared microbial seed liquid with 60 ml of clostridium butyricum liquid, 5 ml of lactobacillus delbrueckii liquid and 55 ml of aspergillus oryzae liquid.
2) The matrix composition is as follows: 500 g of corn flour, 130 g of bran, 70 g of molasses, 70 g of bean pulp, 70 g of rapeseed meal, 70 g of peanut meal, 40 g of wheat protein powder, 30 g of soybean protein concentrate, 8g of xylo-oligosaccharide and 8g of salt, and all the raw materials are mixed and stirred uniformly.
3) Inoculating the zymocyte liquid into a matrix, adding 600 ml of water, and uniformly mixing; placing in a sterile closed fermentation tank, and fermenting at 37 deg.C; the fermentation time is 100 h.
4) After fermentation is finished, the fermented feed is dried and crushed in the air in a natural state, 5% of the fermented feed is added into a preset sow feed to be used as a feed raw material, complete feed is prepared, and the fermented feed is uniformly mixed to obtain the fermented feed special for the lactating sows.
Comparative example 3
1) Preparing a zymophyte liquid: taking the prepared microbial seed liquid, and uniformly mixing according to 30 ml of clostridium butyricum liquid, 30 ml of lactobacillus delbrueckii liquid and 30 ml of aspergillus oryzae liquid, and totaling to 90 ml.
2) The matrix composition is as follows: 300 g of corn flour, 180 g of bran, 40 g of molasses, 90 g of bean pulp, 30 g of rapeseed meal, 120 g of peanut meal, 90 g of wheat protein powder, 5g of soybean protein concentrate, 2g of xylo-oligosaccharide and 15 g of salt, and all the raw materials are mixed and stirred uniformly.
3) Inoculating the zymocyte liquid into a matrix, adding 600 ml of water, and uniformly mixing; placing in a sterile closed fermentation tank, and fermenting at 37 deg.C; the fermentation time is 100 h.
4) After fermentation is finished, the fermented feed is dried and crushed in the air in a natural state, 5% of the fermented feed is added into a preset sow feed to be used as a feed raw material, complete feed is prepared, and the fermented feed is uniformly mixed to obtain the fermented feed special for the lactating sows.
To further illustrate the beneficial effects of the fermented feed additive provided by the present invention in basal feed applications, the following tests were performed:
experimental example 1
240 healthy and large lactating sows with similar body conditions, fetal times, body weights and expected delivery periods are selected in a Henan Crane wall-pig farm and are randomly divided into 8 groups, each group is 3 in number, each group is 10 in number, 7 groups are test groups, the fermented feeds of the groups of the examples 1-3 (S1-3) and the comparative examples 1-3 (D1-3) are respectively fed, and the rest groups are control groups and are fed with base feeds with equivalent energy and protein. The practical feeding effect of the high-energy lactating sow fermented feed is researched, the constipation condition of the sow is recorded before parturition, and the litter size and the live litter size of the sow are recorded after parturition. The balance is settled every 5 days during the test period, and the daily average feed intake of each sow is calculated. And (4) weaning the piglets for 21 days, recording the diarrhea number of the piglets, and calculating the diarrhea rate of the piglets. The data obtained at the end of the test are as follows:
TABLE 1 fermented feed additive lactating sow compound feed
The premix comprises the following components in percentage by weight: 0.15% of multi-mineral for sow, 0.05% of lactation multi-vitamin, 0.1% of 98 lysine, 0.05% of antioxidant, 0.05% of mildew preventive, 0.01% of sweetener, 0.02% of phytase 10000IU, 0.15% of sodium bicarbonate and MgSO40.15 percent, 0.02 percent of VE, 0.002 percent of 1 percent of chromium picolinate, 0.015 percent of yeast selenium and 0.233 percent of zeolite powder.
The whole powder comprises the following components: crude fat is more than or equal to 82 percent, calcium is more than or equal to 6.5 percent, and water content is less than or equal to 10 percent; color: light yellow, total energy 33.6 MJ/kg; the fatty acid component ratio is as follows: 10.4% of palmitic acid, 3.6% of stearic acid, 19.9% of oleic acid, 53.3% of linoleic acid and 7.3% of linolenic acid.
TABLE 2 nutrient composition and pH of the fermented feeds of each group
Note: the difference of lower case letters in the same row represents significant difference (P＜0.05）。
As can be seen from the results of table 2, the fermented feeds provided in examples 1 to 3 of the present invention have higher contents of each nutrient component than the fermented feeds provided in the control group and comparative examples 1 to 3 of the present invention, wherein the fermented feed provided in example 1 of the present invention has the highest content of each nutrient component. The fermented feed provided by the invention has the advantages of rich and comprehensive nutrition by reasonably matching the raw materials, and the content of the nutrient components of the fermented feed prepared by the fermented feed is reduced by changing the content of the raw materials or deleting any raw material.
Experimental example 2
The influence of the fermented feed on the productivity of lactating sows and piglets was examined and the results are shown in table 3.
TABLE 3 influence of feeding fermented feed on various indexes of lactating sows and piglets
Note: the difference of lower case letters in the same row represents significant difference (P＜0.05）。
The above test data show that: the daily average feed intake and lactation yield of the lactating sows are obviously improved by feeding the fermented feed to the lactating sows, and the constipation rate of the lactating sows is obviously reduced; the birth weight, the diarrhea rate and the survival rate of the piglets are obviously better than those of the control group and the comparative group.
Experimental example 3
The influence of the fermented feed on the reproductive performance of lactating sows is detected, and the result is shown in table 4.
TABLE 4 influence of feeding fermented feed on reproductive performance of lactating sows
Note: the difference of lower case letters in the same row represents significant difference (P＜0.05）。
The above test data show that: the fermented feed for the lactating sows obviously improves the litter size of the lactating sows, and shortens the average birth process and the postweaning estrus interval of the sows.
In conclusion, the fermented feed additive provided by the invention can obviously improve the digestibility of the lactating sows to the feed through the reasonable matching of the zymocyte liquid and the matrix. The fermented feed provided by the invention has the advantages of improving the palatability of the feed, improving the nutritional value of the feed, lowering the production cost and fully utilizing various feed raw materials by controlling the reasonable proportion and components of the fermented feed additive and the basic feed, can effectively degrade anti-nutritional factors such as antigen protein and the like in the feed under the condition of not adding antibiotics, better retains the effective components of the feed, simultaneously promotes the intestinal health of sows, enhances the immunity and disease resistance of the sows, effectively improves the constipation of the sows, reduces the morbidity of the sows, improves the breeding rate of piglets, is more favorable for promoting the intestinal health, improves the digestibility of the feed and lightens the pollution to the breeding environment, and has important application prospect for the safe production of animal products.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (7)
1. A fermented feed additive is characterized in that the raw materials of the fermented feed additive consist of a substrate and a zymophyte liquid;
the matrix comprises the following components in parts by weight: 400-600 parts of corn flour, 100-150 parts of wheat bran, 40-80 parts of soybean meal, 60-90 parts of molasses, 40-100 parts of peanut meal, 40-100 parts of rapeseed meal, 10-60 parts of wheat protein powder, 10-50 parts of soybean protein concentrate, 5-10 parts of xylo-oligosaccharide and 5-10 parts of salt; 3-15 parts of zymocyte liquid by weight;
the zymophyte liquid comprises the following components in parts by weight: 1-5 parts of aspergillus oryzae, 1-5 parts of clostridium butyricum and 1-5 parts of lactobacillus delbrueckii;
the concentration of aspergillus oryzae in the zymocyte liquid is 3.27-5.24 log CFU/mL; the concentration of clostridium butyricum is 4.35-5.17 log CFU/mL; the concentration of Lactobacillus delbrueckii is 4.11-5.56 log CFU/mL.
2. The fermented feed additive according to claim 1, wherein the fermented bacterial liquid comprises, in parts by weight: 2-4 parts of aspergillus oryzae, 2-4 parts of clostridium butyricum and 2-4 parts of lactobacillus delbrueckii.
3. The fermented feed additive according to claim 2, wherein the fermented bacterial liquid comprises, in parts by weight: 3 parts of aspergillus oryzae, 3 parts of clostridium butyricum and 3 parts of lactobacillus delbrueckii.
4. A method of preparing a fermented feed additive according to any one of claims 1 to 3, wherein the method of preparation comprises:
and respectively preparing the matrix and the zymophyte liquid according to the formula amount, inoculating the prepared zymophyte liquid into the matrix, uniformly mixing, and fermenting to obtain the fermented feed additive.
5. A fermented feed, characterized in that it comprises the fermented feed additive according to any one of claims 1-3, a basal feed and a whole fat meal.
6. The fermented feed according to claim 5, wherein the fermented feed additive according to any one of claims 1 to 3 is added in an amount of 2 to 5% and the whole fat powder is added in an amount of 1 to 3%.
7. Use of the fermented feed additive of any one of claims 1-3 or the fermented feed of claim 5 or 6 for the preparation of a product for promoting gut health, improving constipation, enhancing resistance and/or increasing piglet growth rate in sows.
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Address after: 476000 Cultural Office of Suiyang District, Shangqiu, Henan, east of Zhenxing Road, Shangqiu eco Food Industrial Park Patentee after: HENAN ZHENGBEN QINGYUAN TECHNOLOGY DEVELOPMENT Co.,Ltd. Address before: 476000 Xinanmen Industrial Zone, Suiyang District, Shangqiu City, Henan Province Patentee before: HENAN ZHENGBEN QINGYUAN TECHNOLOGY DEVELOPMENT Co.,Ltd. |
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Denomination of invention: Fermented bacterial liquid, products containing the same for lactating sows, preparation method and application of products Effective date of registration: 20220114 Granted publication date: 20210709 Pledgee: Bank of China Limited by Share Ltd. Shangqiu branch Pledgor: HENAN ZHENGBEN QINGYUAN TECHNOLOGY DEVELOPMENT Co.,Ltd. Registration number: Y2022980000563 |