CN103621766A - Preparation method and application of biological feed additive with nutrition and immunocompetence - Google Patents

Preparation method and application of biological feed additive with nutrition and immunocompetence Download PDF

Info

Publication number
CN103621766A
CN103621766A CN201310725827.4A CN201310725827A CN103621766A CN 103621766 A CN103621766 A CN 103621766A CN 201310725827 A CN201310725827 A CN 201310725827A CN 103621766 A CN103621766 A CN 103621766A
Authority
CN
China
Prior art keywords
dregs
cfu
fermentation
nutrition
companion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310725827.4A
Other languages
Chinese (zh)
Inventor
田子罡
张列飞
Original Assignee
田子罡
张列飞
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 田子罡, 张列飞 filed Critical 田子罡
Priority to CN201310725827.4A priority Critical patent/CN103621766A/en
Publication of CN103621766A publication Critical patent/CN103621766A/en
Pending legal-status Critical Current

Links

Abstract

The invention discloses a preparation method of a biological feed additive with nutrition and immunocompetence. The biological feed additive with nutrition and immunocompetence comprises the following components: 0.05 to 0.1% of aerobic bacteria, 0.05 to 0.1% of anaerobic bacteria, 0.3 to 0.5% of fermenting promoter, 5 to 15% of soybean meal, 5 to 10% of molasses, 15 to 25% of DDGS (Distillers Dried Grains with Soluble), 10 to 30% of cassava residue, 20 to 50% of bran meal, 5 to 15% of bran, 5 to 15% of maize cob powder, and 20 to 40% of maize slurry protein. The preparation method comprises the processes of activating bacteria, crushing raw materials, aerobically fermenting for 1 to 2 days, and then anaerobically fermenting for 3 to 7 days. The prepared biological feed additive brings piglet, medium and large pigs and sheep with high nutrition value, is high in palatability, and has effects of accelerating growth, improving intestinal health, controlling diarrhea, controlling constipation of sow, increasing birth weight and weaning weight of the piglet, improving the quality of eggshell and egg yolk, controlling feed-containing faeces, raising the lactation level of dairy cattle, as well as increasing butterfat and protein content.

Description

There is the biological feedstuff companion's of nutrition immune activity preparation method and application thereof
Technical field
The present invention relates to feed biological technical field, a kind of preparation method and application thereof with the biological feedstuff companion of nutrition immune activity is provided especially.
Background technology
Micro-ecological active bacteria formulation has regulating intestinal canal colony balance, improves the comprehensive immune level of body, improves the functions such as livestock and poultry production performance.Along with the micro-ecological Studies achievement of animal intestinal constantly gets the nod in Animal Nutrition and Feed Science field, in feed formula, add micro-ecological active bacteria formulation and progressively become normality.But probiotics is in actual breeding production, and its validity is subject to the multifactor impacts such as storage conditions, constant product quality, feeding and management level, cultivating condition, practitioner's quality.In livestock and poultry cultivation is produced, how by solid-state fermentation technology development of new biofermentation activated feed, improve the prebiotic metabolite of probiotic active, accumulating and enriching, make immunizing health function embodiment more direct abundant of probiotics viable bacteria, will inevitably obtain cultivating practitioner's approval.
Biological fermentation feed belongs to feed biotechnology category, is multiple routine or unconventional nutritive value feedstuff on the low side are carried out to probiotics fermention, to eliminate ANFs, improves the utilization ratio of nutriment, improves palatability etc.In fermented feed product, contain the various bioactivators such as abundant probiotics viable bacteria, enzyme, vitamin, polysaccharide.While using this type of fermented feed raw material and ferment complete feed, can when increasing livestock and poultry feed intake and improving feed efficiency, reduce feed cost, improve immunity of organism level, form the market competitiveness.Production technology, mainly by fermentation engineering, enzyme engineering technology etc., improves the utilization rate of protein, reduces content of cellulose, reduces the content of poisonous and harmful substance, accumulating and enriching probio secondary metabolite, thus promote feed quality.More main, in feeding antibiotic application situation increasingly serious today, the application of functional fermentation raw material and fermented feed is for ensureing that animal health is more necessary, and market prospects are very wide.
But vast fermented feed manufacturer has, and infrastructure condition is poor, technical staff professional strong, complicated without testing conditions, the bacterial classification source of science, select the deficiencies such as raw material trophism level is low.And not enough to the prebiotic function and effect mechanism of probio understanding, the nutritional need of the different growth phases of pig, chicken, ox, sheep different animals and intestinal microecology feature are not understood.To such an extent as to fermentation bed material apolegamy specific aim is strong, bacterial classification and fermentation accelerant are arranged in pairs or groups, science, zymotechnique flow process are not unreasonable, the nutritional properties of use procedure Raw itself can not be given full play to, the enteron aisle adjusting of probio, growth promotion, immune enhancing function can not fully demonstrate, and the research and development of this series products and popularization are had to adverse effect.
Summary of the invention
For the problems referred to above, the object of this invention is to provide a kind of preparation method and application technology thereof with the biological feedstuff companion of nutrition immune activity.By fermentative degradation and bio-transformation, ANFs in conventional and unconventional feed is eliminated, macro-molecular protein and SNSP are carried out to external predigestion.Add the compound external source digestive ferment of science assembly simultaneously, improve fermenting speed and transformation efficiency.Select 4 kinds of Bacillus acidi lacticis, 2 kinds of enterococcus, 3 kinds of bacillus, 2 Yeasts, a kind of Aspergillus totally 12 kinds of beneficial microbe cooperative fermentations, the probiotics viable bacteria of accumulating and enriching and secondary metabolite in end-product.The nutrition immune biological feedstuff companion who applies this invention preparation, can significantly improve unconventional feed usage ratio, reduces feed cost, by the prebiotic function of micro-ecological active bacteria formulation and metabolite thereof, improves livestock and poultry production performance.The medicament residues such as the animal products antibiotic-free of producing thus, hormone.The more important thing is that the situation of striving grain, abuse of antibiotics for solving day by day serious people and animals provides an effective solution route.
For achieving the above object, the present invention takes following technical scheme:
The invention provides the compound method of a kind of aerobic fermentation microorganism fungus kind for the preparation of nutrition immune activated feed companion special use, concrete microbe species and proportioning are as follows: bacillus subtilis (100 * 10 8cFU/g) 5-20%, bacillus licheniformis (100 * 10 8cFU/g) 5-20%, bacillus coagulans (100 * 10 8cFU/g) 5-20%, saccharomyces cerevisiae (100 * 10 8cFU/g) 10-20%, candida utili (100 * 10 8cFU/g) 10-20%, aspergillus oryzae (100 * 10 8cFU/g) 10-20%.Said components is mixed according to a certain percentage to packing after pulverizing, packing specification 1kg/ bag.
The invention provides a kind of anaerobic fermentation microorganism fungus kind for the preparation of nutrition immune activated feed companion special use, concrete microbe species and proportioning are as follows: Lactobacillus plantarum (100 * 10 8cFU/g) 20-30%, Lactobacillus casei (100 * 10 8cFU/g) 5-15%, lactobacillus acidophilus (100 * 10 8cFU/g) 10-20%, streptococcus thermophilus (100 * 10 8cFU/g) 10-20%, lactobacillus fermenti (100 * 10 8cFU/g) 10-20%, enterococcus faecalis (100 * 10 8cFU/g) 2-25%.Said components is mixed according to a certain percentage to packing after pulverizing, packing specification 1kg/ bag.
The invention provides a kind of fermentation accelerant for the preparation of nutrition immune activated feed companion special use, concrete component and proportioning are as follows: yeast autolyzate 5-10%, betaine 20-30%, glucose 5-10%, cornstarch 10-30%, calcium carbonate 6-15%, zytase (100000U) 2-5%, phytase (20000U) 5-10%, mannase (50000U) 1-5%, amylase (2000U) 5-10%, carbohydrase (100000U) 5-10%, acid protease (50000U) 2-5%, cellulase (10000U) 2-6%, ammonium dihydrogen phosphate (ADP) 0.5-1.5%, dipotassium hydrogen phosphate 1-3%, sodium chloride 0.2-0.5%, magnesium sulfate 2-5%, Manganous sulfate monohydrate 0.001-0.005%, green vitriol 0.004-0.01%, Salzburg vitriol 0.2-1%.Said components is mixed according to a certain percentage to packing after pulverizing, packing specification 5kg/ bag.
For reaching this object, the invention provides a kind of nutrition immune activated feed companion's for newborn piglet (10-30kg) fermentation bed material mix proportion scheme, concrete raw material composition and mass percent are as follows: dregs of beans (CP=43%) 5-15%, cane molasses 5-10%, import DDGS15-25%, chaff dregs of rice 20-50%, wheat bran 5-15%, maize cob meal 5-15%.After said components is pulverized, cross 20 mesh sieves, mix according to a certain percentage and get final product.
For reaching this object, the invention provides a kind of nutrition immune activated feed companion's for large pig (more than 30kg) fermentation bed material mix proportion scheme, concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 5-10%, cotton dregs (CP=44%) 5-10%, cane molasses 5-10%, manioc waste 15-45%, import DDGS5-15%, chaff dregs of rice 10-30%, wheat bran 5-15%, maize cob meal 5-15%.After said components is pulverized, cross 20 mesh sieves, mix according to a certain percentage and get final product.
For reaching this object, the invention provides a kind of nutrition immune activated feed companion's for sow fermentation bed material mix proportion scheme, concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 10-15%, cane molasses 5-15%, manioc waste 10-25%, import DDGS10-30%, chaff dregs of rice 20-40%, wheat bran 10-20%, maize cob meal 5-10%.After said components is pulverized, cross 20 mesh sieves, mix according to a certain percentage and get final product.
For reaching this object, the invention provides a kind of nutrition immune activated feed companion's for laying hen fermentation bed material mix proportion scheme, concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 5-10%, cane molasses 5-10%, import DDGS10-30%, chaff dregs of rice 15-30%, wheat bran 10-20%, corn whitewashing protein 20-40%, maize cob meal 5-10%, stone flour 5-10%.After said components is pulverized, cross 20 mesh sieves, mix according to a certain percentage and get final product.
For reaching this object, the invention provides a kind of nutrition immune activated feed companion's for lactating cow fermentation bed material mix proportion scheme, concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 5-10%, cotton dregs 5-10%, cane molasses 10-15%, import DDGS5-15%, chaff dregs of rice 10-30%, wheat bran 5-20%, corn whitewashing protein 20-45%, maize cob meal 5-10%.After said components is pulverized, cross 20 mesh sieves, mix according to a certain percentage and get final product.
For reaching this object, the invention provides a kind of for fattening the nutrition immune activated feed companion's of mutton sheep (more than 25kg) fermentation bed material mix proportion scheme, concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 5-10%, cotton dregs 5-10%, cane molasses 5-20%, import DDGS5-15%, chaff dregs of rice 15-30%, wheat bran 10-20%, corn whitewashing protein 20-30%, maize cob meal 5-10%.After said components is pulverized, cross 20 mesh sieves, mix according to a certain percentage and get final product.
The present invention also provides this concrete preparation method with the biological feedstuff companion of nutrition immune activity:
One, actication of culture liquid preparation
Aerobic microbiological bacterial classification 0.5-1kg, is dissolved in the water of proper volume of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Anaerobe bacterial classification 0.5-1kg, is dissolved in the water of proper volume of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Two, raw material is pulverized and mixed
The all feeds raw material of fermentation use is pulverized respectively, and grain fineness is less than 20 orders, drops into according to a certain percentage mixer, under normal temperature, is fully uniformly mixed.
Three, aerobic microbiological inoculation and aerobic fermentation
Described aerobic bacterial classification activating solution is dropped into said mixture in proportion, add fermentation accelerant 3-5kg simultaneously, abundant mix and blend under normal temperature.Material moves into fermentation vat and carries out aerobic fermentation, thickness of feed layer 20-60cm, and each 6-15h stirring stirs, and after fermentation process 24-48h, finishes.
Four, anaerobe inoculation
Material moisture after measuring aerobic fermentation and finishing, drops into said mixture in proportion by described aerobic bacterial classification activating solution, and the clean water of adding appropriate volume is to water content 25-50%, abundant mix and blend under normal temperature.
Five, anaerobic fermentation
The mixture that above-mentioned stirring is completed drops in anaerobic fermentation pond or polyethylene (7-13 silk) packaging bag with one-way exhaust valve, and sealing is fermented, and fermentation temperature 20-40 ℃, ferments and finish for 3-10 days.Gained is the biological feedstuff companion finished product with nutrition immune activity.
After prepared by this product, there is unique fermentation fragrance such as dense alcohol fragrance, sour fragrance, and be rich in the essential digestive ferment of a large amount of animals.In end-product, moisture is 25%-50%, lactic acid content 2-6%, active small peptide 5-8%, about 1-5 * 10 of Bacillus acidi lactici 8cFU/g, enterococcus 0.8-2 * 10 8cFU/g, saccharomycete 0.5-1 * 10 8cFU/g; Cellulase 270-550U/g, protease (acidity) 1250-2000U/g, protease (neutrality) 1500-2200U/g, amylase 2 00-400U/g, zytase 5000-12000U/g, mannase 7500-12500U/g.
Except specified otherwise, the feedstuff the present invention relates to, microorganism fungus kind, enzyme preparation are commercially available.
The present invention, owing to taking above technical scheme, has the following advantages
One, the feature of bacterial classification collocation and zymotechnique
(1) by fermentation, reduce the ANFs in unconventional raw material, improve the absorption efficiency of nutriment, improve unconventional raw material usage ratio, significantly reduce feed cost, alleviate day by day serious people and animals simultaneously and strive grain situation.
(2) after fermentation, have unique fragranced, attractant is splendid.
(3) tunning enrichment natural acid agent--lactic acid, can reduce acidulant consumption in mixed feed; And be rich in the essential digestive ferment of a large amount of animals, contribute to improve complete feed absorption of nutrient ingredients efficiency, reduce feed cost.
(4) sweat is divided into first 2 links of aerobic rear anaerobism, and aerobic fermentation accumulates a large amount of saccharomycete body proteins and abundant active metabolite, and metabolism is simultaneously vigorous, makes material temperature maintain certain level.Anaerobic fermentation reduces pH, the organic acids such as accumulation lactic acid, citric acid, and lactic acid bacteria breeds in a large number, secretes a large amount of prebiotic factors.
(5) fermentation accelerant is rich in multiple digestive ferment, accelerates ANFs and eliminates, and carbohydrate and high molecular weight protein are carried out to external predigestion, for microorganism provides metabolizable energy, improves fermentation efficiency; Yeast growth breeding required various trace elements of stage are provided simultaneously comprehensively, improve growth speed, strengthening metabolite activity, contributes to improve activated feed companion's flavor stability and activity stability.
Two, the effect after feeding animals is remarkable
(1) fermentation raw material is composite scientific and reasonable, balanced in nutrition; Enzymatic production activity is high, and pig, Fattening Sheep, meat-poultry dynamiting is quick, the price of deed is high, physique is healthy and strong; Especially for alleviation, turn group, transportation, wean etc. stress effect remarkable.Egg fowl laying rate is high, and egg size increases, and eggshell strength is large.
(2) after fermentation, feed has unique fermentation acid fragrance and alcohol fragrance, and palatability is fabulous, and pig, ox, sheep search for food vigorous; Be rich in a large amount of active probiotics, about 1-5 * 10 of Bacillus acidi lactici 8cFU/g, enterococcus 0.8-2 * 10 8cFU/g, saccharomycete 0.5-1 * 10 8cFU/g, the quantity of Escherichia coli, salmonella is controlled at 10 3below cfu/g; After feeding, suppress the growth of the harmful bacterias such as the interior Escherichia coli of enteron aisle, salmonella, ensure intestinal microecology balance, reduce pig bacterial diarrhea; Meat egg fowl death rate reduces.
(3) sweat enriched vitamin E, A, D, C and organic trace element, can promote mammary glandular cell propagation, prevents sow, cow breast depauperation; Improve lactation amount, improve butterfat, content of milk protein.Be rich in organic trace elements, egg fowl egg-laying peak extends, and dirty egg rate of broken eggs reduces.
(4) probio and active metabolite stimulating mucosal immunity system thereof, synthesis secretion IL-10, the EGF isoreactivity factor, directly promote body innate immune activity, strengthens premunition.
(5) sweat is removed antigen and ANFs in feed, promotes feed quality, and soluble protein improves 30%-40%; Neutral detergent fiber decline 15%-20%, urea nitrogen reduces by 11%, and protein apparent digestibility improves 10-12%; Effectively solve young animal trophism diarrhea; Tunning is rich in bioactive micro peptide, improves protein utilization rate; Improve unconventional raw material use amount, reduce feed cost, improve cultivation profit;
(6) use after 7-10 days, pig defecation amount obviously reduces, and ight soil is soft, and Fattening Sheep not digest food residue disappears, and in colony house air, indoles, biogenic amine, scatol isoconcentration reduce more than 80%, and breeding environment quality is obviously improved.
the specific embodiment
Below in conjunction with embodiment, to of the present invention, be described in detail.
Embodiment 1
The preparation method who the invention provides a kind of biological feedstuff companion with nutrition immune activity for newborn piglet, the method step is as follows:
One, actication of culture liquid preparation
Aerobic microbiological bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Anaerobe bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Two, raw material is pulverized and mixed
Needed raw material kind and proportioning following (amounting to 1000kg): dregs of beans (CP=43%) 50-150kg, cane molasses 50-100kg, import DDGS150-250kg, chaff dregs of rice 200-500kg, wheat bran 50-150kg, maize cob meal 50-150kg.After said components is pulverized, cross 20-40 mesh sieve, obtain.
Three, aerobic microbiological inoculation and aerobic fermentation
Described aerobic bacterial classification activating solution is dropped into the above-mentioned fermentation raw material for the treatment of, add fermentation accelerant 3-5kg simultaneously, abundant mix and blend under normal temperature.Material moves into fermentation vat and carries out aerobic fermentation, thickness of feed layer 20-60cm, and each 6-15h stirring stirs, and after fermentation process 24-48h, aerobic stage finishes.
Four, anaerobe inoculation
Measure the material moisture after aerobic fermentation finishes, described aerobic bacterial classification activating solution is dropped into said mixture in proportion, regulate water content to 25-50%, abundant mix and blend under normal temperature.
Five, anaerobic fermentation
The mixture that above-mentioned stirring is completed drops in anaerobic fermentation pond or polyethylene (7-13 silk) packaging bag with one-way exhaust valve, and sealing is fermented, and fermentation temperature 20-40 ℃, ferments and finish for 3-10 days.Gained is the biological feedstuff companion finished product with nutrition immune activity.
Six, detoxification
The newborn piglet of preparing according to above-mentioned preparation method is used biological feedstuff companion, and feeding experiment is carried out in Xiaogan City Dawu County, Hubei happy plant of Lee.Select 300 of 35 healthy age in days piglets, be divided at random 3 processed group, each processes 2 repetitions, and each repeats 2 circle pigs, 20 pigs of every circle, and each organizes the initial body weight of piglet without significant difference.The common daily ration of control group fed, feeds after the biological feedstuff companion that test group is added common daily ration 4% ratio mixes.Piglet is fed from 35 ages in days to 55 ages in days, 20 days experimental periods.
Result of the test and analysis
Project Control group Test group 1 Test group 2
Initial weight (kg) 10.25 9.77 9.73
End heavy (kg) 20.03 20.6 20.64
Average weight increasing a day (kg) 0.49 0.55 0.54
Average daily feed intake (kg) 0.84 0.918 0.89
Material anharmonic ratio 1.71 1.68 1.63
Diarrhea rate (%) 0.34 0.253 0.18
Therefore the newborn piglet with nutrition immune activity prepared by the present invention is used biological feedstuff companion, attractant is strong, and piglet feed intake, speed of weight increment are with the obvious advantage; And nutritional labeling is more complete, feed cost obviously reduces, and culture benefit increases; Grice diarrhoea rate reduces, and body's immunity is strengthened.
Embodiment 2
The preparation method who the invention provides a kind of biological feedstuff companion with nutrition immune activity for large pig, the method step is as follows:
One, actication of culture liquid preparation
Aerobic microbiological bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Anaerobe bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Two, raw material is pulverized and mixed
Needed raw material kind and proportioning following (amounting to 1000kg): dregs of beans (CP=43%) 5-10%, cotton dregs (CP=44%) 5-10%, cane molasses 5-10%, manioc waste 15-45%, import DDGS5-15%, chaff dregs of rice 10-30%, wheat bran 5-15%, maize cob meal 5-15%.After said components is pulverized, cross 20-40 mesh sieve, obtain.
Three, aerobic microbiological inoculation and aerobic fermentation
Described aerobic bacterial classification activating solution is dropped into the above-mentioned fermentation raw material for the treatment of, add fermentation accelerant 3-5kg simultaneously, abundant mix and blend under normal temperature.Material moves into fermentation vat and carries out aerobic fermentation, thickness of feed layer 20-60cm, and each 6-15h stirring stirs, and after fermentation process 24-48h, aerobic stage finishes.
Four, anaerobe inoculation
Measure the material moisture after aerobic fermentation finishes, described aerobic bacterial classification activating solution is dropped into said mixture in proportion, regulate water content to 25-50%, abundant mix and blend under normal temperature.
Five, anaerobic fermentation
The mixture that above-mentioned stirring is completed drops in anaerobic fermentation pond or polyethylene (7-13 silk) packaging bag with one-way exhaust valve, and sealing is fermented, and fermentation temperature 20-40 ℃, ferments and finish for 3-10 days.Gained is the biological feedstuff companion finished product with nutrition immune activity.
Six, detoxification
In preparing according to above-mentioned preparation method, large pig is used biological feedstuff companion, feeding experiment is carried out in Xiaogan City Dawu County, Hubei happy plant of Lee, raise in advance 10 days phases (on July 11st, 1 2013 on July 1st, 2013), 60 days experimental periods (on October 10th, 1 2013 on July 12nd, 2013).Select 3O the 90 age in days growing and fattening pigs (40.94 ± 0.095kg) that body weight is close, be divided at random two processing, each processes 3 repetitions, and each repeats 5.Control group daily ration: formed by 63% corn, 22% dregs of beans, 11% wheat bran and 4% premix.On control group basis, by 15%, add fermented feed.Experimental period 30d.Free choice feeding, freely drinks water.Measure feed intake and weightening finish.
Result of the test and analysis
Project Control group Test group
Number (head) 15 15
Initial body weight (kg/ head) 40.9 40.8
Finish body weight (kg/ head) 63.46 65.85
Daily ingestion amount (kg/d) 1.875 2.115
Daily gain (kg/d) 0.752 0.835
Diarrhea rate (%) 16 0
Every of the test group pig 2.39kg that increases weight during off-test, although the total feed consumption rate of test group is greater than control group, because the cost of test group fermented feed is low, every pig reduces 16.5 yuan of feed costs more.Live pig price is calculated by 16 yuan/kg, and every pig increases weight and saves feed and realize 54.74 yuan of total revenues.Therefore, prepared by the present invention have nutrition immune activity in large pig use biological feedstuff companion, attractant is strong, feed intake, speed of weight increment are with the obvious advantage; And nutritional labeling is more complete, feed cost obviously reduces, and culture benefit increases; Diarrhea rate reduces, and body's immunity is strengthened.
Embodiment 3
The preparation method who the invention provides a kind of biological feedstuff companion with nutrition immune activity for sow, the method step is as follows:
One, actication of culture liquid preparation
Aerobic microbiological bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Anaerobe bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Two, raw material is pulverized and mixed
Needed raw material kind and proportioning following (amounting to 1000kg): dregs of beans (CP=43%) 10-15%, cane molasses 5-15%, manioc waste 10-25%, import DDGS10-30%, chaff dregs of rice 20-40%, wheat bran 10-20%, maize cob meal 5-10%.After said components is pulverized, cross 20-40 mesh sieve, obtain.
Three, aerobic microbiological inoculation and aerobic fermentation
Described aerobic bacterial classification activating solution is dropped into the above-mentioned fermentation raw material for the treatment of, add fermentation accelerant 3-5kg simultaneously, abundant mix and blend under normal temperature.Material moves into fermentation vat and carries out aerobic fermentation, thickness of feed layer 20-60cm, and each 6-15h stirring stirs, and after fermentation process 24-48h, aerobic stage finishes.
Four, anaerobe inoculation
Measure the material moisture after aerobic fermentation finishes, described aerobic bacterial classification activating solution is dropped into said mixture in proportion, regulate water content to 25-50%, abundant mix and blend under normal temperature.
Five, anaerobic fermentation
The mixture that above-mentioned stirring is completed drops in anaerobic fermentation pond or polyethylene (7-13 silk) packaging bag with one-way exhaust valve, and sealing is fermented, and fermentation temperature 20-40 ℃, ferments and finish for 3-10 days.Gained is the biological feedstuff companion finished product with nutrition immune activity.
Six, detoxification
The sow of preparing according to above-mentioned preparation method is used biological feedstuff companion, and feeding experiment is carried out in Xiaogan City Dawu County, Hubei happy plant of Lee.Select 46 of multiparity sows, feeding environment is consistent, each 23 of strong and weak similar groupings.The common daily ration of control group fed, test group, on control group basis, is added fermented feed by 8%.After prerun 80 d, start to carry out test data sheet as formal test.Experimental periods 120 d.
Result of the test and analysis
Project Control group Test group
Nest produces average number (head) 13.24 13.32
Piglet birth weight (kg) 1.22 1.27
Weaned piglet heavy (kg) 8.23 8.68
Number of weaned (%) 90.55 94.24
Average litter weight (kg) weans 99.88 108.85
Grice diarrhoea rate (%) 18.5 6.54
Young sow is observed Appetite is normal, movable normal Appetite is strong, vivaciously active
Therefore the sow with nutrition immune activity prepared by the present invention is used biological feedstuff companion, nutritional labeling is more complete, and feed cost obviously reduces; Prevention of sow constipation situation be improved significantly; Lactation, milk was sufficient, and piglet birth weight and weaning weight significantly improve, and grice diarrhoea rate reduces, and body's immunity is strengthened.
Embodiment 4
The preparation method who the invention provides a kind of biological feedstuff companion with nutrition immune activity for laying hen, the method step is as follows:
One, actication of culture liquid preparation
Aerobic microbiological bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Anaerobe bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Two, raw material is pulverized and mixed
Needed raw material kind and proportioning following (amounting to 1000kg): dregs of beans (CP=43%) 5-10%, cane molasses 5-10%, import DDGS10-30%, chaff dregs of rice 15-30%, wheat bran 10-20%, corn whitewashing protein 20-40%, maize cob meal 5-10%, stone flour 5-10%.After said components is pulverized, cross 20-40 mesh sieve, obtain.
Three, aerobic microbiological inoculation and aerobic fermentation
Described aerobic bacterial classification activating solution is dropped into the above-mentioned fermentation raw material for the treatment of, add fermentation accelerant 3-5kg simultaneously, abundant mix and blend under normal temperature.Material moves into fermentation vat and carries out aerobic fermentation, thickness of feed layer 20-60cm, and each 6-15h stirring stirs, and after fermentation process 24-48h, aerobic stage finishes.
Four, anaerobe inoculation
Measure the material moisture after aerobic fermentation finishes, described aerobic bacterial classification activating solution is dropped into said mixture in proportion, regulate water content to 25-50%, abundant mix and blend under normal temperature.
Five, anaerobic fermentation
The mixture that above-mentioned stirring is completed drops in anaerobic fermentation pond or polyethylene (7-13 silk) packaging bag with one-way exhaust valve, and sealing is fermented, and fermentation temperature 20-40 ℃, ferments and finish for 3-10 days.Gained is the biological feedstuff companion finished product with nutrition immune activity.
Six, detoxification
The laying hen of preparing according to above-mentioned preparation method is used biological feedstuff companion, and feeding experiment is carried out in From Shijiazhuang City of Hebei Province Pingshan County plant.Selecting 360 age in days laying rate is 3000, the Luo Man powder of 90% left and right, minute 3 processed group (1 control group and 2 processed group), and 10 repetitions of each processed group, each repeats 100.Each repeats to consist of each 4 chicken coops of upper, middle and lower-ranking, 5 chickens of every cage.Control group fed basal diet, test group 1 and test group 2 are added respectively 2.5% and 5% biological feedstuff companion in basal diet.70 days experimental periods.
Result of the test and analysis
Project Control group Test group Test group
Quantity 1000 1000 1000
Feed intake (g/d/ only) 119.9 119.86 119.93
Laying rate (%) 87.16 88.35 88.11
Egg size (g/) 63.23 64.31 64.22
Broken time egg rate (%) 1.48 1.16 1.25
Dirty egg rate (%) 2.35 1.86 1.67
Yolk color Pale yellow Yellow Yellow
Therefore the laying hen with nutrition immune activity prepared by the present invention is used biological feedstuff companion, nutritional labeling is more complete, and feed cost obviously reduces; Laying rate is stable to be increased, and dirty egg rate, rate of broken eggs situation improve, and body's immunity is strengthened; Egg size increases, and yolk change darkens greatly.
Embodiment 5
The preparation method who the invention provides a kind of biological feedstuff companion with nutrition immune activity for milk cow, the method step is as follows:
One, actication of culture liquid preparation
Aerobic microbiological bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Anaerobe bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Two, raw material is pulverized and mixed
Needed raw material kind and proportioning following (amounting to 1000kg): dregs of beans (CP=43%) 5-10%, cotton dregs 5-10%, cane molasses 10-15%, import DDGS5-15%, chaff dregs of rice 10-30%, wheat bran 5-20%, corn whitewashing protein 20-45%, maize cob meal 5-10%.After said components is pulverized, cross 20-40 mesh sieve, obtain.
Three, aerobic microbiological inoculation and aerobic fermentation
Described aerobic bacterial classification activating solution is dropped into the above-mentioned fermentation raw material for the treatment of, add fermentation accelerant 3-5kg simultaneously, abundant mix and blend under normal temperature.Material moves into fermentation vat and carries out aerobic fermentation, thickness of feed layer 20-60cm, and each 6-15h stirring stirs, and after fermentation process 24-48h, aerobic stage finishes.
Four, anaerobe inoculation
Measure the material moisture after aerobic fermentation finishes, described aerobic bacterial classification activating solution is dropped into said mixture in proportion, regulate water content to 25-50%, abundant mix and blend under normal temperature.
Five, anaerobic fermentation
The mixture that above-mentioned stirring is completed drops in anaerobic fermentation pond or polyethylene (7-13 silk) packaging bag with one-way exhaust valve, and sealing is fermented, and fermentation temperature 20-40 ℃, ferments and finish for 3-10 days.Gained is the biological feedstuff companion finished product with nutrition immune activity.
Six, detoxification
The milk biological feed for cattle companion who prepares according to above-mentioned preparation method, feeding experiment is carried out in Linhe District, Ba Yan Nor City plant; Preliminary trial period, is 6d, and the date is that on November 18th, 2012 was to November 23; Experimental period is 30d, from November 24th, 2012 to December 24.Test several 30, wherein control group is 10, tests 1 group 10, tests 2 groups 10.Same poultry raiser feeds in a cowshed, and the be on dry matter basis quantity of feeding of feed equates, time consistency, and milker is same people.Morning, noon and afternoon feed 3 times altogether, milk 3 times.Test group is added the biological feedstuff companion of 15% ratio on control group fine fodder basis, and companion and roughage are fed after mixing, and freely drink water.
Result of the test and analysis
Project Control group Test group 1 Test group 2
Number 10 10 10
Day is consumed fine fodder amount (kg) 7.7 7.7 7.7
Day is consumed roughage grain (kg) 26.7 26.7 26.7
Daily yielding (kg) 18.19 20.69 20.6
Butterfat percnetage (%) 3.3 3.7 3.73
Content of milk protein (%) 2.65 3.1 3.05
Therefore, the milk biological feed for cattle companion with nutrition immune activity prepared by the present invention, nutritional labeling is more complete, and the output of milk promotes 2.44kg/, and head/more than sky, culture benefit obviously promotes; Ight soil forming degree is good, and body's immunity is strengthened; Lactation level is high, and butterfat percnetage and content of milk protein significantly improve.
Embodiment 6
The preparation method who the invention provides a kind of biological feedstuff companion with nutrition immune activity for agent for rearing and fattening meat sheep, the method step is as follows:
One, actication of culture liquid preparation
Aerobic microbiological bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Anaerobe bacterial classification 0.5-1kg, is dissolved in the clean water of 150-400kg of the about 30-40 of water temperature ℃, intermittent stirring, and activation 2-5h, standby as seed liquor.
Two, raw material is pulverized and mixed
Needed raw material kind and proportioning following (amounting to 1000kg): dregs of beans (CP=43%) 5-10%, cotton dregs 5-10%, cane molasses 5-20%, import DDGS5-15%, chaff dregs of rice 15-30%, wheat bran 10-20%, corn whitewashing protein 20-30%, maize cob meal 5-10%.After said components is pulverized, cross 20-40 mesh sieve, obtain.
Three, aerobic microbiological inoculation and aerobic fermentation
Described aerobic bacterial classification activating solution is dropped into the above-mentioned fermentation raw material for the treatment of, add fermentation accelerant 3-5kg simultaneously, abundant mix and blend under normal temperature.Material moves into fermentation vat and carries out aerobic fermentation, thickness of feed layer 20-60cm, and each 6-15h stirring stirs, and after fermentation process 24-48h, aerobic stage finishes.
Four, anaerobe inoculation
Measure the material moisture after aerobic fermentation finishes, described aerobic bacterial classification activating solution is dropped into said mixture in proportion, regulate water content to 25-50%, abundant mix and blend under normal temperature.
Five, anaerobic fermentation
The mixture that above-mentioned stirring is completed drops in anaerobic fermentation pond or polyethylene (7-13 silk) packaging bag with one-way exhaust valve, and sealing is fermented, and fermentation temperature 20-40 ℃, ferments and finish for 3-10 days.Gained is the biological feedstuff companion finished product with nutrition immune activity.
Six, detoxification
The mutton sheep of fattening of preparing according to above-mentioned preparation method is used biological feedstuff companion, feeding experiment is carried out in Baoding city Tangxian County plant, raise in advance 10 days phases (on August 12nd, 1 2013 on August 2nd, 2013), 45 days experimental periods (on September 27th, 1 2013 on August 13rd, 2013).Test several 90, wherein control group is 30, tests 1 group 30, tests 2 groups 30.Just trying phase feeding experiment daily ration, the Alfalfa 0.4kg that feeds every day, ensiling 0.8kg, fine fodder 0.2kg.Control group fed basal diet, test group is added the biological feedstuff companion of 15% ratio of fine fodder on this basis.Day feed 2 times, measure quantity-unlimitingly, freely drink water.
Result of the test and analysis
Project Control group Test group 1 Test group 2
Number 30 30 30
Initial body weight (kg) 23.65 23.39 22.98
Finish body weight (kg) 32.27 34.51 34.90
Day is consumed fine fodder amount (kg) 0.2 0.2 0.2
Daily gain (g) 0.191 0.247 0.265
Therefore the mutton sheep of fattening with nutrition immune activity prepared by the present invention is used biological feedstuff companion, attractant is strong, and grazing speed, speed of weight increment are with the obvious advantage; And nutritional labeling is more complete, feed cost obviously reduces, and culture benefit increases; Body's immunity is strengthened.
From the probiotics strain of specific function, take into full account general character and the characteristics of personality of probio, then in conjunction with the Diet Formula feature of different animals, form the functional full price fermented feed for different animals moment.Utilize probiotics fermention to promote feed quality, reduce daily ration cost, have broad application prospects.

Claims (9)

1. for the preparation of a compound method for the aerobic fermentation microorganism fungus kind of nutrition immune activated feed companion special use, it is characterized in that: concrete microbe species and proportioning are as follows: bacillus subtilis (100 * 10 8cFU/g) 5-20%, bacillus licheniformis (100 * 10 8cFU/g) 5-20%, bacillus coagulans (100 * 10 8cFU/g) 5-20%, saccharomyces cerevisiae (100 * 10 8cFU/g) 10-20%, candida utili (100 * 10 8cFU/g) 10-20%, aspergillus oryzae (100 * 10 8cFU/g) 10-20%.
2. for the preparation of an anaerobic fermentation microorganism fungus kind for nutrition immune activated feed companion special use, it is characterized in that: concrete microbe species and proportioning are as follows: Lactobacillus plantarum (100 * 10 8cFU/g) 20-30%, Lactobacillus casei (100 * 10 8cFU/g) 5-15%, lactobacillus acidophilus (100 * 10 8cFU/g) 10-20%, streptococcus thermophilus (100 * 10 8cFU/g) 10-20%, lactobacillus fermenti (100 * 10 8cFU/g) 10-20%, enterococcus faecalis (100 * 10 8cFU/g) 2-25%.
3. the fermentation accelerant for the preparation of nutrition immune activated feed companion special use, it is characterized in that: concrete component and proportioning are as follows: yeast autolyzate 5-10%, betaine 20-30%, glucose 5-10%, cornstarch 10-30%, calcium carbonate 6-15%, zytase (100000U) 2-5%, phytase (20000U) 5-10%, mannase (50000U) 1-5%, amylase (2000U) 5-10%, carbohydrase (100000U) 5-10%, acid protease (50000U) 2-5%, cellulase (10000U) 2-6%, ammonium dihydrogen phosphate (ADP) 3-5%, dipotassium hydrogen phosphate 5-10%, sodium chloride 0.2-0.5%, magnesium sulfate 5-10%, Manganous sulfate monohydrate 0.01-0.03%, green vitriol 0.03-0.05%, Salzburg vitriol 0.2-1%.
4. the fermentation bed material mix proportion scheme for the nutrition immune activated feed companion of newborn piglet (10-30kg), it is characterized in that: concrete raw material composition and mass percent are as follows: dregs of beans (CP=43%) 5-15%, cane molasses 5-10%, import DDGS15-25%, chaff dregs of rice 20-50%, wheat bran 5-15%, maize cob meal 5-15%.
5. the fermentation bed material mix proportion scheme for the nutrition immune activated feed companion of large pig (more than 30kg), it is characterized in that: concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 5-10%, cotton dregs (CP=44%) 5-10%, cane molasses 5-10%, manioc waste 15-45%, import DDGS5-15%, chaff dregs of rice 10-30%, wheat bran 5-15%, maize cob meal 5-15%.
6. the fermentation bed material mix proportion scheme for the nutrition immune activated feed companion of sow, it is characterized in that: concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 10-15%, cane molasses 5-15%, manioc waste 10-25%, import DDGS10-30%, chaff dregs of rice 20-40%, wheat bran 10-20%, maize cob meal 5-10%.
7. the fermentation bed material mix proportion scheme for the nutrition immune activated feed companion of laying hen, it is characterized in that: concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 5-10%, cane molasses 5-10%, import DDGS10-30%, chaff dregs of rice 15-30%, wheat bran 10-20%, corn whitewashing protein 20-40%, maize cob meal 5-10%, stone flour 5-10%.
8. the fermentation bed material mix proportion scheme for the nutrition immune activated feed companion of lactating cow, it is characterized in that: concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 5-10%, cotton dregs 5-10%, cane molasses 10-15%, import DDGS5-15%, chaff dregs of rice 10-30%, wheat bran 5-20%, corn whitewashing protein 20-45%, maize cob meal 5-10%.
9. one kind for fattening the nutrition immune activated feed companion's of mutton sheep (more than 25kg) fermentation bed material mix proportion scheme, it is characterized in that: concrete raw material composition and ratio are as follows: dregs of beans (CP=43%) 5-10%, cotton dregs 5-10%, cane molasses 5-20%, import DDGS5-15%, chaff dregs of rice 15-30%, wheat bran 10-20%, corn whitewashing protein 20-30%, maize cob meal 5-10%.
CN201310725827.4A 2013-12-25 2013-12-25 Preparation method and application of biological feed additive with nutrition and immunocompetence Pending CN103621766A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310725827.4A CN103621766A (en) 2013-12-25 2013-12-25 Preparation method and application of biological feed additive with nutrition and immunocompetence

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310725827.4A CN103621766A (en) 2013-12-25 2013-12-25 Preparation method and application of biological feed additive with nutrition and immunocompetence

Publications (1)

Publication Number Publication Date
CN103621766A true CN103621766A (en) 2014-03-12

Family

ID=50203498

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310725827.4A Pending CN103621766A (en) 2013-12-25 2013-12-25 Preparation method and application of biological feed additive with nutrition and immunocompetence

Country Status (1)

Country Link
CN (1) CN103621766A (en)

Cited By (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103829099A (en) * 2014-03-18 2014-06-04 淮北正洋生物科技有限公司 Anti-diarrhea pig feed and preparation method thereof
CN103952346A (en) * 2014-04-17 2014-07-30 青岛九和宜生生物科技有限公司 Preparation of Lactobacillus plantarum/Lactobacillus casei/Bacillus licheniformis composite microbial inoculant
CN103989018A (en) * 2014-05-28 2014-08-20 河南牧业经济学院 Nutritional growth promoting preparation for breeding pheasant and preparation method of preparation
CN104082608A (en) * 2014-06-13 2014-10-08 山东香驰生物饲料有限公司 Biological cooperation grain feed for milking sows and preparation method thereof
CN104171727A (en) * 2014-08-20 2014-12-03 张海员 Preparation method of microbial beneficial living bacterium complete pellet feed for fattening sheep
CN104171744A (en) * 2014-08-28 2014-12-03 寿阳华阳农牧有限公司 Microbial fermentation antibiont-free concentrated feed for pigs
CN104222693A (en) * 2014-10-11 2014-12-24 来光业 Pig feed and producing method thereof
CN104304791A (en) * 2014-10-24 2015-01-28 广西博白扬翔宝中宝饲料有限责任公司 Piglet feed for preventing diarrhea and preparation method of piglet feed
CN104472854A (en) * 2014-11-03 2015-04-01 青岛根源生物技术集团有限公司 Corn enzyme with effect of reducing sugar content increasing, and applications thereof
CN104522294A (en) * 2014-11-27 2015-04-22 苏州嘉禧萝生物科技有限公司 Mixed bacteria composition for fermentation of soybean meal and soybean meal fermented feed
CN104522299A (en) * 2015-01-07 2015-04-22 河南智龙生物科技有限公司 Preparation method of wet-base biological active product
CN104782947A (en) * 2015-03-31 2015-07-22 覃春燕 Feed formula for fattening pigs
CN105039469A (en) * 2015-07-17 2015-11-11 季维峰 Method for preparing compound mycoprotein by organic waste
CN105146079A (en) * 2015-08-11 2015-12-16 桂林鲜牧饲料发展有限公司 Cough-relieving ginkgo nut feed and preparation method thereof
CN105211614A (en) * 2015-09-24 2016-01-06 山东祥维斯生物科技股份有限公司 A kind of betaine type composite fermentation auxiliary agent and the application in fermented bean dregs thereof
CN105360615A (en) * 2015-11-26 2016-03-02 广州市天伟生物科技有限公司 Fermented feed raw material preparation as well as preparation method and application thereof
CN105360644A (en) * 2015-11-26 2016-03-02 广州市天伟生物科技有限公司 Fermented feedstuff preparation for piglets as well as preparation method and application thereof
CN105454693A (en) * 2015-12-24 2016-04-06 辽宁爱琴牧业有限公司 Fermented pig fodder
CN105614094A (en) * 2015-12-22 2016-06-01 广州市天伟生物科技有限公司 Fermented-feed raw material preparation special for pregnant sows and preparing method and application thereof
CN105707478A (en) * 2016-03-11 2016-06-29 山东鹤来生物科技有限公司 Composite probiotic culture and preparation method thereof
CN105767507A (en) * 2016-03-30 2016-07-20 遂平广源饲料科技有限公司 Feed micro-ecological preparation, anti-nutritional substance-free feed and preparation method thereof
CN105941863A (en) * 2016-05-18 2016-09-21 广东海洋大学 Low-fish-meal compound protein source and application thereof to preparation of grouper feed
CN106035991A (en) * 2016-07-22 2016-10-26 上海绿博生物科技发展有限公司 High-protein high-calcium feedstuff prepared from fermented moringa seeds and preparation method of high-protein high-calcium feedstuff
CN106578420A (en) * 2016-12-19 2017-04-26 深圳市前海金卓生物技术有限公司 Fermentation feed and preparation method thereof
CN106615814A (en) * 2016-12-29 2017-05-10 中粮生物科技(北京)有限公司 Feed, preparation method thereof and application of feed to increase of feed intake of lactating sows
CN106954733A (en) * 2016-01-12 2017-07-18 武汉纤然纤维素有限公司 A kind of preparation method and applications of manioc waste activated feed raw material
CN107889954A (en) * 2017-11-28 2018-04-10 佛山宝来迪生物科技有限公司 A kind of pineapple peel viable bacteria feedstuff for live pigs and its preparation and feeding method
CN107963728A (en) * 2017-12-19 2018-04-27 安徽仁维环保工程科技有限公司 A kind of microbial flocculant and preparation method thereof
CN108029853A (en) * 2017-12-01 2018-05-15 河南正本清源科技发展股份有限公司 Zymocyte liquid, comprising its applied to the product of milking sow and the preparation method and application of product
CN108338274A (en) * 2018-02-10 2018-07-31 河南雄峰科技股份有限公司 A kind of milking sow of fermented type and weanling pig concentrated feed and preparation method thereof
CN110100963A (en) * 2019-05-05 2019-08-09 广西壮族自治区畜牧研究所 A kind of Silaging method of sugarcane caudal lobe
CN110663827A (en) * 2018-07-02 2020-01-10 辽宁波尔莱特农牧实业有限公司 Special fermented feed for beef cattle and preparation method thereof
CN110692805A (en) * 2019-10-30 2020-01-17 石家庄飞龙饲料有限公司 Composite probiotic fermented feed for animal breeding and preparation method thereof
CN111328921A (en) * 2020-03-11 2020-06-26 沈阳化工研究院有限公司 Ruminant fermented feed and preparation method thereof
CN111728080A (en) * 2019-03-25 2020-10-02 刘坚 High-protein high-fiber pig feed and application and preparation method thereof
CN112136964A (en) * 2020-09-07 2020-12-29 湖南启农生物科技有限公司 High-protein high-fat biological feed raw material
CN113349287A (en) * 2021-06-23 2021-09-07 宁夏农林科学院荒漠化治理研究所(宁夏防沙治沙与水土保持重点实验室) Caragana microphylla mechanical fermentation type full mixed feed and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1703968A (en) * 2004-05-25 2005-12-07 中国农业大学 Synbiotic feed addictive of mini-peptide with beneficial microbe and its usage

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1703968A (en) * 2004-05-25 2005-12-07 中国农业大学 Synbiotic feed addictive of mini-peptide with beneficial microbe and its usage

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
姜文侠等: "饲用微生态制剂—益生素", 《饲料研究》, vol. 17, no. 2, 31 March 2000 (2000-03-31) *
张日俊: "动物微生态系统的生物防治和营养免疫作用及微生物饲料添加剂的科学使用", 《饲料工业》, vol. 26, no. 16, 31 December 2005 (2005-12-31) *

Cited By (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103829099A (en) * 2014-03-18 2014-06-04 淮北正洋生物科技有限公司 Anti-diarrhea pig feed and preparation method thereof
CN103952346A (en) * 2014-04-17 2014-07-30 青岛九和宜生生物科技有限公司 Preparation of Lactobacillus plantarum/Lactobacillus casei/Bacillus licheniformis composite microbial inoculant
CN103952346B (en) * 2014-04-17 2016-08-31 青岛九和宜生生物科技有限公司 Lactobacillus plantarum, lactobacillus casei and the preparation of Bacillus licheniformis composite bacteria agent capable
CN103989018B (en) * 2014-05-28 2016-02-24 河南牧业经济学院 Pheasant is raised with auxotype growth promotion preparation and preparation method thereof
CN103989018A (en) * 2014-05-28 2014-08-20 河南牧业经济学院 Nutritional growth promoting preparation for breeding pheasant and preparation method of preparation
CN104082608A (en) * 2014-06-13 2014-10-08 山东香驰生物饲料有限公司 Biological cooperation grain feed for milking sows and preparation method thereof
CN104171727A (en) * 2014-08-20 2014-12-03 张海员 Preparation method of microbial beneficial living bacterium complete pellet feed for fattening sheep
CN104171744A (en) * 2014-08-28 2014-12-03 寿阳华阳农牧有限公司 Microbial fermentation antibiont-free concentrated feed for pigs
CN104222693A (en) * 2014-10-11 2014-12-24 来光业 Pig feed and producing method thereof
CN104304791A (en) * 2014-10-24 2015-01-28 广西博白扬翔宝中宝饲料有限责任公司 Piglet feed for preventing diarrhea and preparation method of piglet feed
CN104472854A (en) * 2014-11-03 2015-04-01 青岛根源生物技术集团有限公司 Corn enzyme with effect of reducing sugar content increasing, and applications thereof
CN104522294A (en) * 2014-11-27 2015-04-22 苏州嘉禧萝生物科技有限公司 Mixed bacteria composition for fermentation of soybean meal and soybean meal fermented feed
CN104522299A (en) * 2015-01-07 2015-04-22 河南智龙生物科技有限公司 Preparation method of wet-base biological active product
CN104782947A (en) * 2015-03-31 2015-07-22 覃春燕 Feed formula for fattening pigs
CN105039469A (en) * 2015-07-17 2015-11-11 季维峰 Method for preparing compound mycoprotein by organic waste
CN105146079A (en) * 2015-08-11 2015-12-16 桂林鲜牧饲料发展有限公司 Cough-relieving ginkgo nut feed and preparation method thereof
CN105211614A (en) * 2015-09-24 2016-01-06 山东祥维斯生物科技股份有限公司 A kind of betaine type composite fermentation auxiliary agent and the application in fermented bean dregs thereof
CN105360644A (en) * 2015-11-26 2016-03-02 广州市天伟生物科技有限公司 Fermented feedstuff preparation for piglets as well as preparation method and application thereof
CN105360615A (en) * 2015-11-26 2016-03-02 广州市天伟生物科技有限公司 Fermented feed raw material preparation as well as preparation method and application thereof
CN105360615B (en) * 2015-11-26 2019-05-31 广州市天伟生物科技有限公司 A kind of fermented feed bulk formulation and its preparation method and application
CN105614094A (en) * 2015-12-22 2016-06-01 广州市天伟生物科技有限公司 Fermented-feed raw material preparation special for pregnant sows and preparing method and application thereof
CN105454693A (en) * 2015-12-24 2016-04-06 辽宁爱琴牧业有限公司 Fermented pig fodder
CN106954733A (en) * 2016-01-12 2017-07-18 武汉纤然纤维素有限公司 A kind of preparation method and applications of manioc waste activated feed raw material
CN105707478A (en) * 2016-03-11 2016-06-29 山东鹤来生物科技有限公司 Composite probiotic culture and preparation method thereof
CN105767507A (en) * 2016-03-30 2016-07-20 遂平广源饲料科技有限公司 Feed micro-ecological preparation, anti-nutritional substance-free feed and preparation method thereof
CN105941863B (en) * 2016-05-18 2020-05-29 广东海洋大学 Low-fish-meal composite protein source and application thereof in preparation of grouper feed
CN105941863A (en) * 2016-05-18 2016-09-21 广东海洋大学 Low-fish-meal compound protein source and application thereof to preparation of grouper feed
CN106035991A (en) * 2016-07-22 2016-10-26 上海绿博生物科技发展有限公司 High-protein high-calcium feedstuff prepared from fermented moringa seeds and preparation method of high-protein high-calcium feedstuff
CN106578420A (en) * 2016-12-19 2017-04-26 深圳市前海金卓生物技术有限公司 Fermentation feed and preparation method thereof
CN106615814A (en) * 2016-12-29 2017-05-10 中粮生物科技(北京)有限公司 Feed, preparation method thereof and application of feed to increase of feed intake of lactating sows
CN107889954A (en) * 2017-11-28 2018-04-10 佛山宝来迪生物科技有限公司 A kind of pineapple peel viable bacteria feedstuff for live pigs and its preparation and feeding method
CN108029853A (en) * 2017-12-01 2018-05-15 河南正本清源科技发展股份有限公司 Zymocyte liquid, comprising its applied to the product of milking sow and the preparation method and application of product
CN108029853B (en) * 2017-12-01 2021-07-09 河南正本清源科技发展股份有限公司 Zymophyte liquid, product containing zymophyte liquid and applied to lactating sows, and preparation method and application of product
CN107963728A (en) * 2017-12-19 2018-04-27 安徽仁维环保工程科技有限公司 A kind of microbial flocculant and preparation method thereof
CN108338274A (en) * 2018-02-10 2018-07-31 河南雄峰科技股份有限公司 A kind of milking sow of fermented type and weanling pig concentrated feed and preparation method thereof
CN110663827A (en) * 2018-07-02 2020-01-10 辽宁波尔莱特农牧实业有限公司 Special fermented feed for beef cattle and preparation method thereof
CN111728080A (en) * 2019-03-25 2020-10-02 刘坚 High-protein high-fiber pig feed and application and preparation method thereof
CN110100963B (en) * 2019-05-05 2022-08-30 广西壮族自治区畜牧研究所 Silage method of sugarcane tail leaves
CN110100963A (en) * 2019-05-05 2019-08-09 广西壮族自治区畜牧研究所 A kind of Silaging method of sugarcane caudal lobe
CN110692805A (en) * 2019-10-30 2020-01-17 石家庄飞龙饲料有限公司 Composite probiotic fermented feed for animal breeding and preparation method thereof
CN111328921A (en) * 2020-03-11 2020-06-26 沈阳化工研究院有限公司 Ruminant fermented feed and preparation method thereof
CN112136964A (en) * 2020-09-07 2020-12-29 湖南启农生物科技有限公司 High-protein high-fat biological feed raw material
CN113349287A (en) * 2021-06-23 2021-09-07 宁夏农林科学院荒漠化治理研究所(宁夏防沙治沙与水土保持重点实验室) Caragana microphylla mechanical fermentation type full mixed feed and preparation method thereof

Similar Documents

Publication Publication Date Title
CN103621766A (en) Preparation method and application of biological feed additive with nutrition and immunocompetence
CN103621979A (en) Preparation and application of special compound premix for fermented feed
CN102228129B (en) Fermented feed
CN106834163A (en) Feed fermentation agent, fermented feed and preparation method thereof
CN102488084B (en) Method for preparing protein feed by fermentation on straw and cake using aerobic mixed bacteria
CN100542417C (en) Pig is used fitting-type concentrated feed and production technology and feeding method
CN102018098B (en) Preparation method for fermented feed for corn-bean pulp type daily ration
CN105483040A (en) Compound biological strain for feed and preparation method of fermented feed
CN105614020B (en) A kind of livestock and poultry Alfalfa microbial forage additive and preparation method thereof
CN104886390A (en) Biologically fermented pig feed and a preparation method
CN102907563B (en) Method for preparing high-activity probiotic preparation for livestock breeding
CN104000021B (en) The working method of a kind of vegetalitas fermented feed
CN101186896A (en) Fermentation material for composite microorganism and preparation method thereof
CN105707434A (en) Method and application of fermented biological feed prepared by multi-strain mixed fermentation
CN100998369A (en) Bo-gen double micro-organism protein forage
CN101744149A (en) High-performance environment-friendly cattle feed and preparation method thereof
CN102870990A (en) Sow fermented feed and production method thereof
CN105166422A (en) Biological fermentation feed for accelerating pig growth and preparation method thereof
CN105767575A (en) Green laying-hen feed formula capable of reducing dirty egg rate and preparation method thereof
CN104886392A (en) Biological fermentation pig feed replacing antibiotics, and preparation method of biological fermentation pig feed
CN108142728A (en) Fish fermented feed and its preparation method and application
CN106954733A (en) A kind of preparation method and applications of manioc waste activated feed raw material
CN105146057A (en) Chicken manure biological fermentation and feed preparation technology
CN105166324B (en) A method of utilizing ramie core culture medium mushroom bran Fodder making
CN111938036A (en) Biological fermentation feed additive for nursery pigs and preparation and use methods thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C05 Deemed withdrawal (patent law before 1993)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20140312