CN105211614A - A kind of betaine type composite fermentation auxiliary agent and the application in fermented bean dregs thereof - Google Patents
A kind of betaine type composite fermentation auxiliary agent and the application in fermented bean dregs thereof Download PDFInfo
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Abstract
The invention discloses a kind of betaine type composite fermentation auxiliary agent, the application of composite fermentation auxiliary agent in fermented bean dregs, betaine type composite fermentation auxiliary agent comprises following material composition: the FeSO47H2O of the betaine of 90 ~ 98.5%, the CuSO45H2O of 0.1 ~ 0.8%, the MnSO4H2O of 0.2 ~ 1%, the ZnSO47H2O of 0.5 ~ 5% and 0.5 ~ 5%.By mass percent be the dregs of beans of 60 ~ 80%, the wheat bran of 15 ~ 35% and 5 ~ 10% sucrose composition dregs of beans culture high-temperature sterilization, by bacillus subtilis bacterium liquid access fermentation tank in, fermentation 48 ~ 60h; Then according to the composite fermentation auxiliary agent fermentation 36 ~ 48h of access lactic acid bacterial liquid and 1.0 ~ 6.0wt%, fermentation ends drying, sieve and obtain fermented bean dregs finished product.The present invention is applied in bean pulp fermentation, improves ferment effect, ensure that fermented bean dregs steady quality.
Description
Technical field
The invention belongs to fermentation technical field, be specifically related to betaine type composite fermentation auxiliary agent and the application in fermented bean dregs thereof, particularly the preparation of betaine fermentation assistant of the Coenzyme Complex factor and the application in fermented bean dregs thereof.
Background technology
Dregs of beans is a kind of byproduct obtained after soybean is squeezed soybean oil.In soybean, protein content is very high, and to carry out in course of oil processing, protein losses is little, and therefore, the protein content in dregs of beans is also very high, and it is one of known the abundantest plant protein resource.Although dregs of beans because of its protein content high, amino acid balance is good, and particularly the content of lysine is higher, is a kind of excellent Plant protein feed source, become one of indispensable good protein feedstuff of livestock and poultry cultivation, especially fish and the good dietary protein source of nonruminant.But, there is multiple ANFs in dregs of beans simultaneously, mainly contain trypsin inhibitor, phytic acid, soybean lectin, urase, compound sugar, lipoxidase, Soybean antigen protein (sensitizing factor) and goitrin etc.The existence of these ANFs hinders livestock and poultry to the digestion of bean-dregs feed nutrient, absorption and metabolism, produces harmful effect in various degree to animal health and production performance, also limit its application percentage in the daily ration such as fish and nonruminant.
At present, the method eliminating ANFs in dregs of beans is both at home and abroad more, and sum up multiple methods such as mainly containing physics, chemistry, Biology Breeding, additive and biotechnology, numerous method respectively has its pluses and minuses.Also a lot of according to the limiting factor that the known enzymatic isolation method of practical condition utilizing enzymatic isolation method to prepare bean-dregs feed for many years exists, the kind being mainly used for the enzyme of enzymatic isolation method is few, so just greatly limit the development of bean-dregs feed industry; In addition, because the kind of the protease for hydrolysis is few, can not good degrading soybean protein, the Soybean Peptide bitter taste produced can not be removed at all, thus affect feed mouthfeel, cause when carrying out animal feeding greatly being reduced by letting animals feed food ration.
Compared with enzymatic isolation method, microbe fermentation method has shortening fermentation period, reduces the advantages such as the production cost of bean-dregs feed, has vast potential for future development.Solid state fermentation can realize more large-scale production, and the production cost preparing bean-dregs feed obviously reduces; Substantially produce without to the refuse of environment in solid ferment process.Therefore, solid state fermentation will occupy more and more important position in feed processing industry.Fermentation method has been proved to be as a kind of of bioremediation the better method reducing the trypsin inhibitor factor.Some microbial enzymes on the one hand by producing in fermentation process, eliminate ANFs residual in dregs of beans.Protein on the other hand in dregs of beans is become little peptide by the protease hydrolytic that microorganism produces, and the little peptide produced after hydrolysis has good dissolubility, anti-gel formative, low viscosity and digests and assimilates the characteristics such as fast, can further improve dregs of beans nutritive value.
Fermented bean dregs has another name called biological soybean meal, for primary raw material with high-quality dregs of beans, under multiple bacteria compound fermentation, microorganism is utilized to be small molecular protein and little peptide in the enzymolysis of the multiple protein enzyme secreted in journey that grows by the breaks down proteins of macromolecule, in fermented bean dregs degrading soybean protein process, produce a large amount of probio simultaneously, oligopeptides, glutamic acid, lactic acid, vitamin, the materials such as UGF (UGF), and utilize the enzymolysis of protease by inhibiting factors such as the trypsin inhibitor of soybean protein, a kind of nutritious plant protein fodder that the ANFs such as haemoglutinin are eliminated as much as possible and obtained.Research shows, when not affecting feeding effect, fermented bean dregs partly can replace import high-quality fish meal, whey powder in feed formula, remarkable reduction Feed Manufacturing cost, in fermented bean dregs, soybean protein has been decomposed into the easily digested Soybean Peptide absorbed in a large number, therefore can well overcome by the letting animals feed shortcoming more weak to phytoprotein digestion power, promote that animal better grows, shortens breeding cycle, improves measurement techniques for quality detection of meat, greatly reduce feeding cost, bring better economic benefit.
Although in recent years, multiple bacteria compound fermentation method fermented bean dregs is utilized to prepare bean-dregs feed for the ANFs removed in dregs of beans and inhibitor, thus the nutritive value aspect improving dregs of beans compares deep research, solve the many problems existed with the inhibitor in other method removal dregs of beans and ANFs, in the inhibitor removed in dregs of beans and ANFs, achieve obvious progress.But, produced problem mainly unstable product quality in present fermented bean dregs production, even the fermented bean dregs quality of same producer different batches also differs greatly, except each producer adopts different strain, different process, cause that fermented bean dregs difference in quality is large, the main cause of poor stability is the bad operation of large scale fermentation, material skewness is exactly lack extensive, the measured fermented bean dregs process for solid state fermentation of matter in addition.
Betaine is a kind of quaternary amine type alkaloid, is extensively present in animals and plants, especially the highest with content in beet blackstrap.It is as a kind of surfactant, can reduce material surface interfacial tension, increase material mixture homogeneity; In addition, betaine can effective adjusting ambient osmotic pressure, ensures that the bioenzyme activity produced in one-step fermentation is unaffected; And the minor metallic element additive in composite fermentation auxiliary agent, meet the constant supply of the required various minor metallic element of fermentation, and can promote to form amino acid and metallo-chelate.Chinese patent CN102660520A discloses a kind of Nutrious fermented auxiliary agent, nutritional auxiliary zytase preparation in application and application process, its auxiliary agent consists of anhydrous betaine 80g/L, vitamin A 0.1g/L, vitaminB10 .4g/L, vitamin B6 0.05g/L, vitamin B12 0.15g/L, vitamine D3 0.1g/L, vitamin E 0.6g/L, Vitamin K3 0.1g/L and nicotinic acid 0.8g/L, effectively can promote the growth of aspergillus niger spore suspension thalline and the metabolism of zytase, significantly improve the output of ferment effect and zytase, shorten fermentation time, reduce production cost.Chinese patent CN102719504A disclose a kind of vitamin B12 composite fermentation auxiliary agent and vitamin B12 fermentation in application process, comprise the betaine of 90 ~ 98%, the B B-complex of 0.15 ~ 0.55% and 1.45 ~ 9.85% compound amino acid; Described B B-complex comprise the thiamine of mass percent 30 ~ 55%, the puridoxine hydrochloride of 10 ~ 25% and 40 ~ 55% folic acid; Described compound amino acid comprise mass percent 5 ~ 10% lysine, 10 ~ 15% serine, the glutamic acid of 10 ~ 15%, the asparagine of 30 ~ 35% and 30 ~ 40% aspartic acid.This auxiliary agent can ensure the constant supply of various trace element, reduces the fermentation problem because organic carbon nitrogenous source medium trace element instability causes, effectively can promote fermentation yield.But, although all betaine is applied as fermentation assistant in above-mentioned document, all fail to relate to and use in bean pulp fermentation field.How betaine is applied to bean pulp fermentation field, providing a kind of and can significantly improve the fermented bean dregs stay-in-grade betaine type composite fermentation auxiliary agent that ferment effect, the material fermentation uniformity are high, produce, is problem demanding prompt solution of the present invention.
Summary of the invention
The object of the invention is to provide a kind of being applied in bean pulp fermentation can significantly improve the fermented bean dregs stay-in-grade betaine type composite fermentation auxiliary agent that ferment effect, the material fermentation uniformity are high, produce, and the application and methods for using them of described composite fermentation auxiliary agent in fermented bean dregs.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of betaine type composite fermentation auxiliary agent, described composite fermentation auxiliary agent at least comprises the material composition of following mass percent: the FeSO47H2O of the betaine of 90 ~ 98.5%, the CuSO45H2O of 0.1 ~ 0.8%, the MnSO4H2O of 0.2 ~ 1%, the ZnSO47H2O of 0.5 ~ 5% and 0.5 ~ 5%.
Preferably, described composite fermentation auxiliary agent comprises the material composition of following mass percent: 91 ~ 98% betaines, CuSO45H2O, the MnSO4H2O of 0.4 ~ 0.8% of 0.1 ~ 0.8%, the FeSO47H2O of the ZnSO47H2O of 0.5 ~ 3% and 1 ~ 5%.
Preferably, described betaine is the mixture of one or more in anhydrous betaine, a water betaine, hydrochloride betaine, phosphate betaine, sulphonic acid betaine.
Preferably, in described betaine, the content of residual amine is 0.001% ~ 0.1%.
Present invention also offers a kind of above-mentioned application of betaine type composite fermentation auxiliary agent in fermented bean dregs.
Preferably, described composite fermentation auxiliary agent is joined in fermentation medium with the addition of 1.0 ~ 6.0wt% of fermentation medium ferment.
Present invention also offers a kind of method of fermented bean dregs, i.e. the application process of betaine type composite fermentation auxiliary agent in fermented bean dregs, comprise following steps:
(1) dregs of beans culture preparation: be the dregs of beans of 60 ~ 80% by mass percent, the wheat bran of 15 ~ 35% and 5 ~ 10% the dregs of beans culture of sucrose composition mix, load fermentation tank, add water and make material-water ratio reach 0.6 ~ 1.2, carry out high-temperature sterilization;
(2) fermented and cultured: by bacillus subtilis bacterium liquid according in the inoculum concentration access fermentation tank of 2 ~ 5wt%, stir after fermentation; Then according to the inoculum concentration access lactic acid bacterial liquid of 2 ~ 5wt%, stir, the composite fermentation auxiliary agent described in one of claim 1-4 is sprayed onto in dregs of beans culture by the addition of 1.0 ~ 6.0wt% and ferments;
(3) drying and crushing: by the material after fermentation ends after 40 ~ 70 DEG C dry, pulverize and sieve and obtain fermented bean dregs finished product.
Preferably, in described step (2), fermentation of bacillus subtilis condition is 25 ~ 30 DEG C of fermentation 48 ~ 60h.
Preferably, in described step (2), the fermentation condition of composite fermentation auxiliary agent is 32 ~ 37 DEG C of fermentation 36 ~ 48h.
Preferably, in described step (1), sample-loading amount during described dregs of beans culture loading fermentation tank is 1/4 ~ 1/3.
Preferably, the concentration of described bacillus subtilis bacterium liquid is 10
7~ 10
9individual/ml; The concentration of described lactic acid bacterial liquid is 10
7~ 10
9individual/ml.
Preferably, described composite fermentation auxiliary agent is added in dregs of beans culture after micro-filtration is degerming.
Preferably, described high-temperature sterilization refers at 115 DEG C of high-temperature sterilization 20min.
The present invention has beneficial effect as described below:
Composite fermentation auxiliary agent of the present invention comprises following material composition: the FeSO47H2O of the betaine of 90 ~ 98.5%, the CuSO45H2O of 0.1 ~ 0.8%, the MnSO4H2O of 0.2 ~ 1%, the ZnSO47H2O of 0.5 ~ 5% and 0.5 ~ 5%, wherein betaine is a kind of quaternary amine type alkaloid, extensively be present in animals and plants, especially the highest with content in beet blackstrap.It is as a kind of surfactant, can reduce material surface interfacial tension, increase material mixture homogeneity; In addition, betaine can effective adjusting ambient osmotic pressure, ensures that the bioenzyme activity produced in one-step fermentation is unaffected; And the minor metallic element additive in composite fermentation auxiliary agent, meet the constant supply of the required various minor metallic element of fermentation, and can promote to form amino acid and metallo-chelate.
Therefore, composite fermentation auxiliary agent of the present invention is applied in bean pulp fermentation, is not affecting the constant supply that can ensure various minor metallic element in bean pulp fermentation process within the scope of spawn activity on the one hand, promote to form amino-acid metal chelate; Material table surface tension, the improving material uniformity can be reduced on the other hand, ensure fermented bean dregs steady quality.In addition, adopt two-step fermenting during this bean pulp fermentation, improve ferment effect, greatly improve fermentation efficiency, shorten fermentation time.
Detailed description of the invention
For better the present invention being described, the present invention can be explained in more detail by the following examples.The present invention is not limited to the following examples.
Embodiment 1
Betaine type composite fermentation auxiliary agent comprises the material composition of following mass percent: the FeSO47H2O of the anhydrous betaine of 91.7%, the CuSO45H2O of 0.8%, the MnSO4H2O of 0.5%, the ZnSO47H2O of 2% and 5%.
Embodiment 2
Betaine type composite fermentation auxiliary agent comprises the material composition of following mass percent: the FeSO47H2O of the hydrochloride betaine of 95%, the CuSO45H2O of 0.2%, the MnSO4H2O of 0.8%, the ZnSO47H2O of 1.5% and 2.5%, in described betaine, the content of residual amine is 0.001%.
Embodiment 3
Betaine type composite fermentation auxiliary agent comprises the material composition of following mass percent: the FeSO47H2O of the phosphate betaine of 98%, the CuSO45H2O of 0.1%, the MnSO4H2O of 0.4%, the ZnSO47H2O of 0.5% and 1%, in described betaine, the content of residual amine is 0.1%.
Embodiment 4
Betaine type composite fermentation auxiliary agent comprises the material composition of following mass percent: the mixture of the sulphonic acid betaine of 93%, CuSO45H2O, the MnSO4H2O of 0.5% of 0.5%, the FeSO47H2O of the ZnSO47H2O of 3% and 3%, in described betaine, the content of residual amine is 0.008%.
Embodiment 5
Be the dregs of beans of 60% by mass percent, the wheat bran of 35% and 5% the dregs of beans culture of sucrose composition mix, load fermentation tank, add water and make material-water ratio reach 0.6, at 115 DEG C of high-temperature sterilization 20min.
By concentration be 107/ml bacillus subtilis bacterium liquid according to 5wt% inoculum concentration access fermentation tank in, after stirring 25 DEG C fermentation 60h; Then be the lactic acid bacterial liquid of 107/ml according to the inoculum concentration access concentration of 5wt%, stir, the addition by 6.0% after composite fermentation auxiliary agent micro-filtration embodiment 1 prepared is degerming is sprayed onto in dregs of beans culture, at 32 DEG C of fermentation 48h.
By the material after fermentation ends after 40 DEG C dry, pulverize and sieve and obtain fermented bean dregs finished product.
Embodiment 6
Be the dregs of beans of 70% by mass percent, the wheat bran of 25% and 5% the dregs of beans culture of sucrose composition mix, the sample-loading amount according to 1/3 loads fermentation tank, adds water and makes material-water ratio reach 0.9, at 115 DEG C of high-temperature sterilization 20min.
By concentration be 108/ml bacillus subtilis bacterium liquid according to 3wt% inoculum concentration access fermentation tank in, after stirring 28 DEG C fermentation 52h; Then be the lactic acid bacterial liquid of 108/ml according to the inoculum concentration access concentration of 3wt%, stir, the addition by 4.5% after composite fermentation auxiliary agent micro-filtration embodiment 2 prepared is degerming is sprayed onto in dregs of beans culture, at 34 DEG C of fermentation 45h.
By the material after fermentation ends after 58 DEG C dry, pulverized 1.2mm and sieved and obtained fermented bean dregs finished product.
Embodiment 7
Be the dregs of beans of 75% by mass percent, the wheat bran of 18% and 7% the dregs of beans culture of sucrose composition mix, the sample-loading amount according to 1/4 loads fermentation tank, adds water and makes material-water ratio reach 1.2, at 115 DEG C of high-temperature sterilization 20min.
By concentration be 109/ml bacillus subtilis bacterium liquid according to 2wt% inoculum concentration access fermentation tank in, after stirring 30 DEG C fermentation 48h; Then be the lactic acid bacterial liquid of 109/ml according to the inoculum concentration access concentration of 2wt%, stir, the addition by 2.0% after composite fermentation auxiliary agent micro-filtration embodiment 3 prepared is degerming is sprayed onto in dregs of beans culture, at 37 DEG C of fermentation 36h.
By the material after fermentation ends after 70 DEG C dry, pulverized 1.2mm and sieved and obtained fermented bean dregs finished product.
Embodiment 8
Be the dregs of beans of 70% by mass percent, the wheat bran of 20% and 10% the dregs of beans culture of sucrose composition mix, the sample-loading amount according to 1/3 loads fermentation tank, adds water and makes material-water ratio reach 1.0, at 115 DEG C of high-temperature sterilization 20min.
By concentration be 108/ml bacillus subtilis bacterium liquid according to 3wt% inoculum concentration access fermentation tank in, after stirring 28 DEG C fermentation 54h; Then be the lactic acid bacterial liquid of 108/ml according to the inoculum concentration access concentration of 3wt%, stir, the addition by 4.0% after composite fermentation auxiliary agent micro-filtration embodiment 4 prepared is degerming is sprayed onto in dregs of beans culture, at 35 DEG C of fermentation 42h.
By the material after fermentation ends after 60 DEG C dry, pulverized 1.2mm and sieved and obtained fermented bean dregs finished product.
Comparative example 1
As different from Example 6, the addition of composite fermentation auxiliary agent is 9.0% to the present embodiment, and all the other are identical.
Be the dregs of beans of 70% by mass percent, the wheat bran of 25% and 5% the dregs of beans culture of sucrose composition mix, the sample-loading amount according to 1/3 loads fermentation tank, adds water and makes material-water ratio reach 0.9, at 115 DEG C of high-temperature sterilization 20min.
By concentration be 108/ml bacillus subtilis bacterium liquid according to 3wt% inoculum concentration access fermentation tank in, after stirring 28 DEG C fermentation 52h; Then be the lactic acid bacterial liquid of 108/ml according to the inoculum concentration access concentration of 3wt%, stir, the addition by 9% after composite fermentation auxiliary agent micro-filtration embodiment 2 prepared is degerming is sprayed onto in dregs of beans culture, at 34 DEG C of fermentation 45h.
By the material after fermentation ends after 58 DEG C dry, pulverized 1.2mm and sieved and obtained fermented bean dregs finished product.
Comparative example 2
As different from Example 6, the addition of composite fermentation auxiliary agent is 0 to the present embodiment, and all the other are identical.
Be the dregs of beans of 70% by mass percent, the wheat bran of 25% and 5% the dregs of beans culture of sucrose composition mix, the sample-loading amount according to 1/3 loads fermentation tank, adds water and makes material-water ratio reach 0.9, at 115 DEG C of high-temperature sterilization 20min.
By concentration be 108/ml bacillus subtilis bacterium liquid according to 3wt% inoculum concentration access fermentation tank in, after stirring 28 DEG C fermentation 52h; Then be the lactic acid bacterial liquid of 108/ml according to the inoculum concentration access concentration of 3wt%, stir, at 34 DEG C of fermentation 45h.
By the material after fermentation ends after 58 DEG C dry, pulverized 1.2mm and sieved and obtained fermented bean dregs finished product.
To in embodiment 5-8, comparative example 1-2, the indexs such as fermented bean dregs color, smell, viscosity, PD degree are monitored, the impact of result composite fermentation additive dosage on test the results are shown in Table 1, and in composite fermentation auxiliary agent, the impact of betaine kind on test the results are shown in Table 2.
Table 1 composite fermentation additive dosage is on the impact of test
"+": represent high-quality degree
In table 2 composite fermentation auxiliary agent, betaine kind is on the impact of test
"+": represent high-quality degree
According to the result of table 1, use the composite fermentation auxiliary agent that the present invention adopts, fermented bean dregs color, smell, viscosity can be made all can to reach preferably level, and protein degradation is thorough.On the contrary, when not adding composite fermentation auxiliary agent of the present invention, the fermented bean dregs color obtained, smell, levels of viscosity are general, and protein degradation is not thorough.Illustrate, use composite fermentation auxiliary agent of the present invention, can ferment effect be improved, ensure fermented bean dregs steady quality.
According to the result of table 2, use the composite fermentation auxiliary agent that the present invention adopts, adopt different types of betaine that fermented bean dregs color, smell, viscosity can be made all can to reach preferably level, protein degradation is thorough.Illustrate, when using composite fermentation auxiliary agent of the present invention, betaine kind is not limited to the concrete kind such as anhydrous betaine, hydrochloride betaine, phosphate betaine, sulphonic acid betaine, all can improve ferment effect, ensure fermented bean dregs steady quality.
The part not in the detailed description of the invention prior art that maybe can adopt same as the prior art is realized.
Patent of the present invention is not limited to above-mentioned disclosed embodiment, and all belong to all changes of the embodiment in this design and scope and improve all within protection scope of the present invention.
Claims (10)
1. a betaine type composite fermentation auxiliary agent, is characterized in that: described composite fermentation auxiliary agent at least comprises the material composition of following mass percent: the FeSO47H2O of the betaine of 90 ~ 98.5%, the CuSO45H2O of 0.1 ~ 0.8%, the MnSO4H2O of 0.2 ~ 1%, the ZnSO47H2O of 0.5 ~ 5% and 0.5 ~ 5%; Preferably comprise the material composition of following mass percent: 91 ~ 98% betaines, CuSO45H2O, the MnSO4H2O of 0.4 ~ 0.8% of 0.1 ~ 0.8%, the FeSO47H2O of the ZnSO47H2O of 0.5 ~ 3% and 1 ~ 5%.
2. betaine type composite fermentation auxiliary agent as claimed in claim 1, is characterized in that: described betaine is the mixture of one or more in anhydrous betaine, a water betaine, hydrochloride betaine, phosphate betaine, sulphonic acid betaine; In preferred described betaine, the content of residual amine is 0.001% ~ 0.1%.
3. the application of betaine type composite fermentation auxiliary agent in fermented bean dregs as described in one of claim 1-2.
4. the application of betaine type composite fermentation auxiliary agent in fermented bean dregs as claimed in claim 3, is characterized in that: joined in fermentation medium with the addition of 1.0 ~ 6.0wt% of fermentation medium by described composite fermentation auxiliary agent and ferment.
5. a method for fermented bean dregs, is characterized in that comprising following steps:
(1) dregs of beans culture preparation: be the dregs of beans of 60 ~ 80% by mass percent, the wheat bran of 15 ~ 35% and 5 ~ 10% the dregs of beans culture of sucrose composition mix, load fermentation tank, add water and make material-water ratio reach 0.6 ~ 1.2, carry out high-temperature sterilization;
(2) fermented and cultured: by bacillus subtilis bacterium liquid according in the inoculum concentration access fermentation tank of 2 ~ 5wt%, stir after fermentation; Then according to the inoculum concentration access lactic acid bacterial liquid of 2 ~ 5wt%, stir, the composite fermentation auxiliary agent described in one of claim 1-2 is sprayed onto in dregs of beans culture by the addition of 1.0 ~ 6.0wt% and ferments;
(3) drying and crushing: by the material after fermentation ends after 40 ~ 70 DEG C dry, pulverize and sieve and obtain fermented bean dregs finished product.
6. the method for a kind of fermented bean dregs as claimed in claim 5, is characterized in that: in described step (2), fermentation of bacillus subtilis condition is 25 ~ 30 DEG C of fermentation 48 ~ 60h; In preferred described step (2), the fermentation condition of composite fermentation auxiliary agent is 32 ~ 37 DEG C of fermentation 36 ~ 48h.
7. the method for a kind of fermented bean dregs as described in one of claim 5-6, is characterized in that: in described step (1), and sample-loading amount during described dregs of beans culture loading fermentation tank is 1/4 ~ 1/3.
8. the method for a kind of fermented bean dregs as described in one of claim 4-7, is characterized in that: the concentration of described bacillus subtilis bacterium liquid is 10
7~ 10
9individual/ml; The concentration of described lactic acid bacterial liquid is 10
7~ 10
9individual/ml.
9. the method for a kind of fermented bean dregs as described in one of claim 4-8, is characterized in that: added after micro-filtration is degerming in dregs of beans culture by described composite fermentation auxiliary agent.
10. the method for a kind of fermented bean dregs as described in one of claim 4-9, is characterized in that: described high-temperature sterilization refers at 115 DEG C of high-temperature sterilization 20min.
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| CN105962360A (en) * | 2016-05-09 | 2016-09-28 | 山东祥维斯生物科技股份有限公司 | Method for producing microorganism ferment |
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| CN109527221A (en) * | 2019-01-07 | 2019-03-29 | 吕锋 | Saccharomycete base compound micro-ecological preparation containing Valine and preparation method thereof |
| CN111264711A (en) * | 2020-03-27 | 2020-06-12 | 辽宁九州生物科技有限公司 | Chinese herbal medicine fermented feed and preparation process thereof |
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