KR20090087974A - A making method of feed additive from whole rubus coreanus miquel fermentation - Google Patents

A making method of feed additive from whole rubus coreanus miquel fermentation Download PDF

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KR20090087974A
KR20090087974A KR1020080013240A KR20080013240A KR20090087974A KR 20090087974 A KR20090087974 A KR 20090087974A KR 1020080013240 A KR1020080013240 A KR 1020080013240A KR 20080013240 A KR20080013240 A KR 20080013240A KR 20090087974 A KR20090087974 A KR 20090087974A
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bokbunja
feed additive
fermentation
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KR1020080013240A
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Korean (ko)
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김기호
김혜자
이기남
김석환
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김기호
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
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Abstract

A method of manufacturing fermented feed additives is provided to improve the quality of a feedstuff by feeding fermented feedstuff to an domestic animal. A feed additive base is manufactured by mixing a rubi fructus of 10 weight% ~40 weight%, rubi fructus leaf, a stem of 10 weight% ~40 weight%, a rice bran of 10 weight% ~30 weight%, sugar of 5 weight% ~15 weight%, ginseng cake of 1 weight% ~10 weight%, and hardwood charcoal of 0.5 weight% ~5 weight%. The lactobacillus sp and saccharomyces sp are vaccinated to a MRS broth and a Potato Dextrose Broth and are cultivated under 24‹C~37‹C within 24 hours ~72 hour and pre-culture medium is manufactured.

Description

복분자박, 복분자잎, 줄기 발효 사료 첨가제의 제조방법{A making method of feed additive from whole Rubus coreanus miquel fermentation}A making method of feed additive from whole Rubus coreanus miquel fermentation

본 발명은 복분자 추출 부산물인 복분자박과 복분자잎, 줄기를 이용한 기능성 사료 첨가제에 관한 것으로 더욱 상세하게는 복분자박과 복분자잎, 줄기를 건조하여 인삼박, 미강, 참숯, 설탕 등을 첨가하고 유산균과 효모 전배양액을 접종하여 혐기적 상태에서 배양시켜 만든 기능성 사료 첨가제의 제조방법에 관한 것이다. The present invention relates to a functional feed additive using the bokbunja extract and bokbunja leaves, stems, which are by-products of bokbunja extract, and more specifically, ginseng leaves, rice bran, charcoal, sugar, etc. It relates to a method for producing a functional feed additive made by incubating in the anaerobic state by inoculating the yeast preculture.

복분자는 항산화 작용, 콜레스테롤 및 간, 위장 기능개선 등의 약리적 효과가 있으며 복분자의 추출 후에 발생되는 복분자박에도 소량의 유용성분이 함유되어 있고 특히 복분자잎에는 혈압강하, 항산화 작용 등의 약리성분이 함유되어 있다. 또한 인삼박, 미강, 참숯 등의 유용성분을 극대화시키기 위하여 설탕을 첨가한 후 유산균과 효모 혼합 전배양액을 접종시켜 10℃~35℃에서 수일 이상 혐기적으로 발효시킴으로서 효모, 유산균 등의 유용미생물이 함유된 생균 제제 함유 기능성 사료 첨가제를 개발할 수 있는 방법에 관한 것이다.Bokbunja has pharmacological effects such as antioxidant activity, cholesterol, liver, and gastrointestinal function improvement, and small amounts of useful ingredients are also contained in Bokbunja Park, which is generated after extracting Bokbunja. have. In addition, in order to maximize useful ingredients such as ginseng meal, rice bran, and charcoal, sugar is added, and then inoculated with lactic acid bacteria and yeast mixed pre-culture solution, and fermented anaerobicly for more than several days at 10 ℃ ~ 35 ℃ to make useful microorganisms such as yeast and lactic acid bacteria. The present invention relates to a method for developing a functional feed additive containing a probiotic preparation.

본 발명에서는 식품가공 부산물인 복분자박과 복분자잎, 줄기, 인삼박,미강, 참숯, 설탕 등의 유용성분의 구성비와 배합방법, 유산균, 효모 혼합배양액의 전 배양 방법, 유산균과 효모 배양액을 접종하여 혐기적 상태에서 저온 발효 숙성시키는 방법 등에 관한 관련 기술을 연구 검토하였다.In the present invention, by inoculating the composition ratio and blending method of useful components such as bokbunja Park and bokbunja leaf, stem, ginseng gourd, rice bran, sesame charcoal, sugar, food processing by-products, pre-culture method of lactic acid bacteria, yeast mixed culture medium, lactic acid bacteria and yeast culture solution Related technologies related to the method of fermentation at low temperature in anaerobic conditions were studied.

소, 돼지, 닭 등 사료 급이 효율을 극대화 시키고 양질의 육질이 형성될 수 있도록 하기 위하여 항산화 작용 및 콜레스테롤 강하, 간, 위장 및 장기능 개선 등의 효과가 원료를 선별하여 각각의 기능별 유용성분을 배합하고 흡수율과 유기산 등 영양성분을 향상시키기 위하여 유산균과 효모의 혼합배양 방법을 개발하여 본 발명에 의한 사료 첨가제의 제조방법에 관한 기술을 확립하였다.In order to maximize feed efficiency such as cattle, pigs, and chickens, and to ensure high quality meat quality, antioxidants, cholesterol lowering, liver, gastrointestinal function, etc. In order to blend and improve the absorption rate and nutrients such as organic acids, a method of producing a feed additive according to the present invention was established by developing a mixed culture method of lactic acid bacteria and yeast.

본 발명에 의한 사료 첨가제의 제조방법에 있어서는 복분자박과 복분자잎, 줄기의 유요 기능성분을 활용하여 상호 상승효과가 있는 식품 부산물류를 탐색하여 그 종류와 배합비율을 확립하고 배합된 원료의 영양 기능 성분을 배가시키기 위한 유산균과 효모 전배양액의 제조방법 및 유산균, 효모 전배양액을 접종하여 혐기적으로 저온 발효시키는 방법을 개발하고자 한다.In the manufacturing method of the feed additive according to the present invention, by utilizing the active functional ingredients of bokbunja Park, bokbunja leaves, stems to search for food by-products having a synergistic effect, to establish the type and ratio of nutrition and the nutritional function of the blended raw materials It is intended to develop a method for producing lactic acid bacteria and yeast preculture solution to double the components and a method of inoculating anaerobic low temperature fermentation by inoculating lactic acid bacteria and yeast preculture solution.

본 발명은 복분자박, 복분자잎, 줄기를 주요 성분으로 구성하여 인삼박, 미강, 참숯 등을 혼합 조제하는 단계, 상기 단계 후 유산균과 효모 혼합 발효 전배양액을 첨가하여 발효시키는 단계로 구성하여 각종 영양 성분 및 기능 성분을 극대화시키는 과정을 특징으로 하고 있다.The present invention comprises a step of preparing a mixture of ginseng gourd, rice bran, sesame charcoal, etc. by the main components consisting of bokbunja bakja, bokbunja leaves, stems, and fermentation by adding a pre-cultured fermentation broth mixed with lactic acid bacteria after the step It is characterized by a process that maximizes the ingredients and functional ingredients.

본 발명에 의한 복분자박과 복분자잎, 줄기를 이용한 사료 첨가제는 식품 부산물인 복분자박과 복분자잎, 줄기, 인삼박, 미강, 참숯 등의 유용 기능성 부산물을 활용하여 사료 첨가제의 원료로 사용함으로서 자원의 재활용 효과가 기대되며 유산균과 효모의 혼합발효 방법을 확립함으로서 유기산, 유산균 등에 의한 생리활성 기능성을 극대화 시킬 수 있는 효과도 있게 된다.Feed additives using bokbunja Park, bokbunja leaves, stems according to the present invention is a raw material of feed additives by using the useful functional by-products such as bokbunja Park and bokbunja leaves, stem, ginseng gourd, rice bran, charcoal, etc. Recycling effect is expected, and by establishing a mixed fermentation method of lactic acid bacteria and yeast, there is also an effect that can maximize the physiological activity functionality by organic acids, lactic acid bacteria and the like.

아울러 가축의 사료급이 효율을 향상시키고 고품질의 육류를 생산할 수 있게 되어 축산 산업의 고부가 가치를 실현할 수 있는 효과가 있게 된다.In addition, the feed grade of livestock can improve the efficiency and produce high quality meat, which is effective in realizing the high value of livestock industry.

본 발명에 의한 복분자박과 복분자잎, 줄기를 이용한 사료 첨가제의 제조방법은 복분자박과 복분자잎, 줄기를 주 원료로 하여 인삼박, 미강, 참숯, 설탕 등의 부재료를 혼합하는 단계, 생리활성과 기능성을 배가시키기 위하여 MRS broth와 PDB 배지에서 24℃~37℃로 각각 유산균과 효모 전배양액을 만드는 단계, 상기 복분자박과 복분자잎, 줄기 함유 사료 첨가제에 유산균과 효모 전배양액을 10%~20% 접종시켜 10℃~35℃에서 혐기적으로 발효 숙성시키는 단계로 구성되어 있다.Method for preparing feed additives using bokbunja bokbunja, bokbunja leaves, stems according to the present invention is a step of mixing the ingredients, such as ginseng gourd, rice bran, charcoal, sugar using bokbunja park, bokbunja leaves, stems as main raw materials, In order to double the functionality, to prepare the lactic acid bacteria and yeast preculture solution at 24 ℃ ~ 37 ℃ in MRS broth and PDB medium, respectively, lactic acid bacteria and yeast preculture solution to the feed additive containing bokbunja and bokbunja leaves, stems Inoculation fermentation is anaerobic at 10 ℃ ~ 35 ℃ consists of the step.

본 발명의 구성에 관한 사항을 실시 예를 통하여 설명하면 다음과 같다.The matters relating to the configuration of the present invention will be described through the following embodiments.

1) 복분자박 10~40 중량%, 복분자잎,줄기 10~40 중량%, 미강 10~30 중량%, 설탕 5~15 중량%, 인삼박 1~10 중량%, 참숯 0.5~5 중량%를 혼합하여 사료 첨가제 베이스를 만든다.1) Mix 10 ~ 40% by weight of bokbunja, mix 10 ~ 40% by weight of bokbunja leaves, 10 ~ 30% by weight of rice bran, 5 ~ 15% by weight of sugar, 1 ~ 10% by weight of ginseng, 0.5 ~ 5% by weight of charcoal To make a feed additive base.

2) MRS, PDB 액체배지에 유산균(Lactobacillus sp .)과 효모(Saccharomyces sp.)를 각각 0.1~10 중량%씩 접종하여 24℃~37℃에서 24~72시간 배양하여 전배양액을 만든다.2) Lactobacillus in MRS, PDB liquid medium sp . ) And yeast ( Saccharomyces sp. ) Are inoculated with 0.1 to 10% by weight of each incubation at 24 ℃ to 37 ℃ for 24 to 72 hours to make a preculture.

3) 상기 1)에 상기 2)를 5~25 중량%를 접종한다.3) Inoculate 5 to 25% by weight of 1) to 2).

4) 상기 3)을 10℃~35℃에서 24시간 이상 혐기적으로 발효 숙성시킨다. 4) The fermentation and fermentation of 3) anaerobic at least 24 hours at 10 ℃ ~ 35 ℃.

5) 상기 4)를 정선, 정량, 포장하여 기능성 사료 첨가제를 얻는다. 5) Select, quantify and package 4) above to obtain a functional feed additive.

상기 복분자박, 복분자잎, 줄기 발효 사료 첨가제 50g에 정제수 1kg을 가하여 99℃에서 8시간 열수 추출 후 원심분리한 다음 상등액을 감압 농축 동결건조하여 항산화 효과를 알아보기 위하여 SOD-like activity(SOD 유사활성) 실험을 수행였으며 시료의 농도는 1mg/ml, 5mg/ml, 10mg/ml, 20mg/ml로 하여 Marklund의 방법 을 이용하여 측정하였다.1 kg of purified water was added to 50 g of the bokbunja park, bokbunja leaf and stem fermented feed additives, extracted with hot water at 99 ° C. for 8 hours, and then centrifuged. The concentration of the sample was measured using Marklund's method as 1mg / ml, 5mg / ml, 10mg / ml, 20mg / ml.

실험결과 모든 농도에서 80% 이상의 항산화 효과가 있는 것으로 나타났으며이는 다른 시료들을 이용한 실험 결과와 비교해 볼 때 상당히 높은 것으로 판단되었다.Experimental results showed that there was an antioxidant effect of more than 80% at all concentrations, which was considered to be considerably high compared with the results of experiments using other samples.

concentration (unit %) concentration (unit%) 20mg/ml  20mg / ml 10mg/ml 10mg / ml 5mg/ml 5mg / ml 1mg/ml 1mg / ml 95.42  95.42 94.54 94.54 92.15 92.15 82.24 82.24

본 발명에서의 복분자박, 복분자잎, 줄기 함유 유산균, 효모 발효 사료 첨가제는 복분자박, 복분자잎, 줄기, 인삼박, 미강 등의 식품 부산물을 이용하여 유산균과 효모 발효 숙성에 의한 고 기능성 시료 첨가제를 제공할 수 있게 해 준다.Bokbunja Park, Bokbunja Leaf, Stem-Containing Lactic Acid Bacteria, Yeast Fermentation Feed Additive is a high functional sample additive by fermentation of lactic acid bacteria and yeast fermentation using food by-products such as bokbunja park, bokbunja leaf, stem, ginseng and rice bran Enable to provide

따라서 저가의 식품 부산물 원부재료를 이용하여 값이 싸고 생리활성이 있는 사료 첨가제를 제공해 줄 수 있게 되어 소, 돼지, 닭 등의 사육에 있어 사료급이 효율을 극대화 시킬 수 있게 하고 고품질의 육류를 생산할 수 있게 되어 고부가 가치 축산업에 이용될 수 있게 된다.Therefore, it is possible to provide inexpensive and physiologically active feed additives using raw food by-products of low cost, so that feed grade can maximize efficiency in breeding cattle, pigs, and chickens and produce high quality meat. It can be used for high value-added livestock industry.

도 1은 본 발명에 의한 사료 첨가제의 제조 공정도 및 항산화 실험 결과1 is a manufacturing process chart and antioxidant test results of the feed additive according to the present invention

Claims (1)

복분자박, 복분자잎, 줄기 함유 유산균, 효모 발효 사료 첨가제 기능성 사료 첨가제에 있어서Bokbunja Park, Bokbunja Leaf, Lactobacillus-containing Lactic Acid Bacteria, Yeast Fermented Feed Additives 1) 복분자박 10중량%~40중량%, 복분자잎, 줄기 10중량%~40중량%, 미강 10 중량%~30중량%, 설탕 5중량%~15중량%, 인삼박 1중량%~10중량%, 참숯 0.5중량%~5중량%를 혼합하여 사료 첨가제 베이스를 만드는 단계1) 10% to 40% by weight of bokbunja, 10% to 40% by weight of bokbunja leaf, stem, 10% to 30% by weight of rice bran, 5% to 15% by weight of sugar, 1% to 10% by weight of ginseng leaf %, 0.5% to 5% by weight of charcoal to make a feed additive base 2) MRS, PDB 액체배지에 유산균(Lactobacillus sp .)과 효모(Saccharomyces sp.)를 각각 0.1중량%~10중량%씩 접종하여 24℃~37℃에서 24시간~72시간 배양하여 전배양액을 만드는 단계2) Lactobacillus in MRS, PDB liquid medium sp . ) And inoculating 0.1% by weight to 10% by weight of Saccharomyces sp. , Respectively, and incubating at 24 ° C. to 37 ° C. for 24 hours to 72 hours to form a preculture solution. 3) 상기 1)에 상기 2)를 5중량%~25중량%를 접종하는 단계3) inoculating 1) 5% to 25% by weight of 2) 4) 상기 3)을 10℃~35℃에서 24시간 이상 혐기적으로 발효 숙성시키는 단계 5) 상기 1)~4)의 제조 공정을 특징으로 하여 마들어 지는 사료 첨가제 또는 그 제조 방법4) fermentatively aged 3) at 10 ° C. to 35 ° C. for anaerobic fermentation for 24 hours or more.
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Publication number Priority date Publication date Assignee Title
KR101372400B1 (en) * 2013-06-20 2014-03-07 재단법인 금산국제인삼약초연구소 A manufacturing method of red ginseng staf4h with enhanced trace ginsenoside
KR101444416B1 (en) * 2012-08-14 2014-09-30 한국방송통신대학교 산학협력단 Pharmaceutical composition for preventing or treating infectious diseases by pathogenic microorganism comprising extract of Rubus coreanus branch as effective component
CN104877921A (en) * 2015-05-27 2015-09-02 广州环亚化妆品科技有限公司 Saccharomycetes culture medium and preparation method thereof
KR20190071207A (en) * 2017-12-14 2019-06-24 재단법인 전라북도생물산업진흥원 Feed composition for tenebrio molitor using agricultural byproducts and breeding method of tenebrio molitor using it

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101444416B1 (en) * 2012-08-14 2014-09-30 한국방송통신대학교 산학협력단 Pharmaceutical composition for preventing or treating infectious diseases by pathogenic microorganism comprising extract of Rubus coreanus branch as effective component
KR101372400B1 (en) * 2013-06-20 2014-03-07 재단법인 금산국제인삼약초연구소 A manufacturing method of red ginseng staf4h with enhanced trace ginsenoside
CN104877921A (en) * 2015-05-27 2015-09-02 广州环亚化妆品科技有限公司 Saccharomycetes culture medium and preparation method thereof
KR20190071207A (en) * 2017-12-14 2019-06-24 재단법인 전라북도생물산업진흥원 Feed composition for tenebrio molitor using agricultural byproducts and breeding method of tenebrio molitor using it

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