CN103989018A - Nutritional growth promoting preparation for breeding pheasant and preparation method of preparation - Google Patents

Nutritional growth promoting preparation for breeding pheasant and preparation method of preparation Download PDF

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CN103989018A
CN103989018A CN201410230150.1A CN201410230150A CN103989018A CN 103989018 A CN103989018 A CN 103989018A CN 201410230150 A CN201410230150 A CN 201410230150A CN 103989018 A CN103989018 A CN 103989018A
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preparation
pheasant
growth promotion
auxotype
solution
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CN103989018B (en
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刘卫东
黄炎坤
程璞
宋素芳
马伟
崔锦
王章存
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Henan University of Animal Husbandry and Economy
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Henan University of Animal Husbandry and Economy
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Abstract

The invention discloses a nutritional growth promoting preparation for breeding pheasant and a preparation method of the preparation, which aim at solving the technical problem that the growth speed is low in the breeding process of the pheasant. The preparation comprises the following raw materials in parts by mass: 40 to 55 parts of chick-pea protein peptide, 20 to 25 parts of wheat hydrolyzed protein, 15 to 25 parts of pea protein peptide, 0.5 to 1 part of compound enzyme, 0.2 to 0.4 part of phytase and 7 to 12 parts of corn starch. The preparation method comprises the steps: uniformly mixing the compound enzyme, phytase and corn starch; then uniformly mixing the mixture with other ingredients. The nutritional growth promoting preparation has beneficial effects that when the growth promoting preparation is added to pheasant feed, the digestive absorption of pheasant for nutritional substances in the feed can be promoted, the utilization rate of the nutritional substances can be increased, the concentration of the nutritional substances in plasma of pheasant can be increased, the chicken of the bred pheasant is tender, fresh and delicious, the nutritional value is high, no toxicity or side effect exists, no residue exists, and no adverse effect on the health of the human body is produced.

Description

Pheasant is raised with auxotype growth promotion preparation and preparation method thereof
Technical field
The present invention relates to Animal nutrition technical field, be specifically related to a kind of pheasant and raise with auxotype growth promotion preparation and preparation method thereof.
Background technology
Not only meat cunning is tender, local flavor is delicious for pheasant, and there is the amino acid of being rich in, lack the advantages such as fatty and cholesterol, but and there is the tonifying Qi of breathing heavily, the medicinal efficacy such as tired, the removing heat from the lung to relieve cough of phlegmization only, be just used as since ancient times promote longevity, the health-care good product of healthcare.Along with people's growth in the living standard and the improvement of economic condition, people's dietary structure also changes occurring gradually, people pursue health, pursue embodiment fresh, that pursuit game has become one way of life quality now, thereby pheasant has been subject to increasing consumer's favor, so the pheasant of artificial feeding has wide market prospects.But the speed of growth of pheasant is slow, growth cycle is long, the cost that causes pheasant to be raised is high, and these problems become the bottleneck of restriction pheasant raising industry development.Some manufacturing enterprise adopts the growth promoter of antibiotic as pheasant, although there is certain effect, antibiotic toxic side effect, easily along with pheasant product introduction human body, produces even teratogenesis, carcinogenic, mutagenesis of drug resistance, the serious threat mankind's health.Therefore, the growth promotion preparation of studying a kind of Nantural non-toxic noresidue improves the speed of growth of pheasant, reduces feeding cost, and the development of pheasant being raised to industry has great importance.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of pheasant natural, that have no side effect that promotes pheasant growth and raises with auxotype growth promotion preparation and preparation method thereof.
For solving the problems of the technologies described above, pheasant of the present invention is raised with auxotype growth promotion preparation, in mass fraction, its raw materials comprises: 40~55 parts Chickpea Protein peptide, 20~25 parts of hydrolyzed wheat proteins, 15~25 parts of pea protein peptides, 0.5~1.0 part of complex enzyme, 0.2~0.4 part of phytase, 7~12 parts of cornstarch.
Preferably, described complex enzyme is made up of zytase, dextranase, cellulase, acid protease, neutral proteinase, amylase, seminase, pectase.
Preferably, in described complex enzyme, the enzyme amount of various enzymes is: zytase 18000~22000 U/g, dextranase 20000~30000U/g, cellulase 6000~7000U/g, acid protease 2500~3500U/g, neutral proteinase 1000~2000U/g, amylase 3000~4000U/g, seminase 3000~4000U/g, pectase 2000~3000U/g.
Preferably, the enzyme amount of described phytase is 4500~5500U/g.
Preferably, the addition of described growth promotion preparation is 1.5~6% of pheasant feeding quality.
Preferably, the addition of described growth promotion preparation is 2~2.5% of pheasant feeding quality.
Pheasant of the present invention is raised the preparation method with auxotype growth promotion preparation, comprises the following steps:
(1), in mass fraction, choose following raw materials: 40~55 parts Chickpea Protein peptide, 20~25 parts of hydrolyzed wheat proteins, 15~25 parts of pea protein peptides, 0.5~1.0 part of complex enzyme, 0.2~0.4 part of phytase, 7~12 parts of cornstarch;
(2) first complex enzyme, phytase are mixed with cornstarch;
(3) mixture of step (2) gained is mixed with remaining other component make pheasant auxotype growth promotion preparation again.
The preparation method of described Chickpea Protein peptide is:
A. the Chickpea Protein powder suspension that is 8~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0, reacts 2.5~3 hours;
B. in the solution of steps A gained, add the HCl of 1mol/L to adjust its pH to 7.0, then add neutral enzymatic hydrolysis 1.5~2 hours by 0.3~0.5% of solution quality;
C. step B gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, being then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal being processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain Chickpea Protein peptide.
The preparation method of described hydrolyzed wheat protein is:
A. the Gluten aaerosol solution that is 9~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0~8.3 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0~8.3, reacts 2.5 ~ 3 hours;
B. in the solution of step a gained, add the HCl of 1mol/L to adjust its pH to 7.0, then add food flavor enzyme hydrolysis 1.5~2 hours by 0.2~0.3% of solution quality;
C. step b gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, being then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal being processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain hydrolyzed wheat protein.
The preparation method of described pea protein peptide is:
1. the Fed Protein Powder of Pea Insteal aaerosol solution that is 9~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0~8.3 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0~8.3, reacts 2.5 ~ 3 hours;
2. 1. in the solution of gained, add the HCl of 1mol/L to adjust its pH to 7.0 to step, then add trypsin hydrolysis 1.5~2 hours by 0.2~0.3% of solution quality;
3. by step 2. gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, be then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain hydrolyzed wheat protein.
Beneficial effect of the present invention is:
1. in pheasant feed, add this growth promotion preparation, can promote the utilization rate of digesting and assimilating, improve nutriment of pheasant to nutriment in feed, increase the concentration of nutriment in pheasant serum, the pheasant delicious meat of raising, be of high nutritive value.
2. this growth promotion preparation can be applicable to each stage of pheasant growth, applied widely, can effectively improve the speed of growth, shortening breeding cycle, the reduction pheasant feeding cost of pheasant.
3. this growth promotion preparation adopts natural material to be prepared from, and does not have any toxic and side effect, noresidue, can not have a negative impact to health.
4. its preparation method is simple, production cost is low, and the addition in feed is few, can further save feeding cost.
Detailed description of the invention
Below in conjunction with embodiment, the specific embodiment of the present invention is described, but following examples are just used for describing in detail the present invention, and limit the scope of the invention never in any form.
The proportioning (mass fraction) of according to the form below 1 listed each embodiment is prepared pheasant and is raised with auxotype growth promotion preparation.
Wherein, complex enzyme is made up of zytase, dextranase, cellulase, acid protease, neutral proteinase, amylase, seminase, pectase.
In complex enzyme, the enzyme amount of various enzymes is: zytase 18000~22000 U/g, dextranase 20000~30000U/g, cellulase 6000~7000U/g, acid protease 2500~3500U/g, neutral proteinase 1000~2000U/g, amylase 3000~4000U/g, seminase 3000~4000U/g, pectase 2000~3000U/g.
The enzyme amount of phytase is 4500~5500U/g.
This pheasant raising comprises the following steps: by the preparation method of auxotype growth promotion preparation
(1) according to the raw material proportioning of the listed each embodiment of table 1, choose corresponding raw material;
(2) first complex enzyme, phytase are mixed with cornstarch;
(3) mixture of step (2) gained is mixed with remaining other component make pheasant auxotype growth promotion preparation again.
The preparation method of this Chickpea Protein peptide is:
A. the Chickpea Protein powder suspension that is 8~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0, reacts 2.5~3 hours;
B. in the solution of steps A gained, add the HCl of 1mol/L to adjust its pH to 7.0, then add neutral enzymatic hydrolysis 1.5~2 hours by 0.3~0.5% of solution quality;
C. step B gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, being then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal being processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain Chickpea Protein peptide.
The preparation method of this hydrolyzed wheat protein is:
A. the Gluten aaerosol solution that is 9~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0~8.3 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0~8.3, reacts 2.5 ~ 3 hours;
B. in the solution of step a gained, add the HCl of 1mol/L to adjust its pH to 7.0, then add food flavor enzyme hydrolysis 1.5~2 hours by 0.2~0.3% of solution quality;
C. step b gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, being then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal being processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain hydrolyzed wheat protein.
The preparation method of this pea protein peptide is:
1. the Fed Protein Powder of Pea Insteal aaerosol solution that is 9~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0~8.3 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0~8.3, reacts 2.5 ~ 3 hours;
2. 1. in the solution of gained, add the HCl of 1mol/L to adjust its pH to 7.0 to step, then add trypsin hydrolysis 1.5~2 hours by 0.2~0.3% of solution quality;
3. by step 2. gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, be then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain hydrolyzed wheat protein.
In above embodiment, related instrument and equipment if no special instructions, is routine instrument device; The related raw material of industry if no special instructions, is the commercially available conventional raw material of industry.
The present invention use raw material in, hydrolyzed wheat protein is the product of Gluten through enzymolysis, its gross protein value is up to 80%, amino acid content is abundant, especially glutamine content exceedes 30%, glutamine is nitrogen transfer between body tissue, the important carrier that ammonia transmits, also Shi Chang Dot theca cell, lymphocyte, vascular endothelial cells etc. are the main energy sources material of proliferative cell fast, glutamine has the cell of promotion fast breeding, strengthen the effect of Immune Function In Animals, it also participates in the synthetic of the interior reducing agent GSH of body, can strengthen body and remove free radical, anti-oxidative damage and maintain cyto-architectural function, under stress situation, if do not maintain the concentration of blood glutamine, and only improve the nitrogen balance in body, not only effect is not remarkable, even may cause serious harm to the immune system of body.Chickpea Protein peptide and pea protein peptide all belong to small peptide material, little peptide not only can directly be absorbed in enteron aisle, and because different from amino acid whose mechanism of absorption, little peptide is in the time that enteron aisle is absorbed, exist and itself be difficult for the advantage that saturated, transport speed is fast, can alleviate intestines parietal cell different free amino acids are taken in competition, thereby can accelerate little peptide and amino acid whose absorption, improve amino acid whose concentration in blood, thereby accelerate the synthetic of protein.And the amino acid in hydrolyzed wheat protein, Chickpea Protein peptide and pea protein peptide has complementarity.
Secretion of phytase not in pheasant body, phytic acid contained in plant cannot be degraded to inositol and phosphoric acid, also be difficult to by the mineral element of phytic acid complexing and protein release out, and in feed, add the phytase phytic acid of just degrading, forming phosphoric acid is utilized by pheasant, by by the mineral element of phytic acid complexing and protein release out, improve their digestibility simultaneously; The decomposition utilization that in complex enzyme, contained cellulase, protease, lipase and amylase dextranase, zytase, pectase also can improve corresponding substrate, and complex enzyme and phytase, improving on the digestibility and utilization of nutriment, have the effect of mutually promoting.
Below in conjunction with specific experiment, effect of the present invention is described.
Test 1: pheasant of the present invention is raised the impact on pheasant growth indexes and blood parameters with auxotype growth promotion preparation.
(1) experimental design: select 600 of 4 Zhou Lingliuhua pheasant healthy chicks, be divided at random 5 groups, establish 3 for every group and repeat 40 chickens of each repetition, male and female half and half.1 week preliminary trial period, just trying at the beginning of phase from the 5th week age of pheasant, finished to 16 week age Mo, control group is fed basal diet, test 1~4 group and feeds respectively corresponding test daily ration, tests the composition of daily ration and trophic level in table 2.(wherein, the growth promotion preparation in experiment daily ration is prepared by the proportioning of the embodiment of the present invention 1.) in experimental period, pheasant free choice feeding and drinking-water, feeding and management and immune programme for children carry out according to normal feeding process.Test data is carried out statistical analysis with SPSS11.0 statistical software, when significant difference, carries out multiple ratio by SSR method, and test data represents by average ± standard deviation.
Wherein, compound premix provides 1.5 milligrams of methionine, 6.6 grams of calcium, 1.8 grams of Phos for every kilogram of daily ration, 3 grams of salt, 70 milligrams, manganese, 60 milligrams, zinc, 6 milligrams of copper, 80 milligrams of iron, 0.2 milligram, selenium, 0.3 milligram of iodine, 0.5 gram, choline, vitamin A 3 000 IU, neo dohyfral D3 1500 IU, vitamin E 30 IU, 3 milligrams of vitamin Ks, 70 milligrams, nicotinic acid, 10 milligrams, pantothenic acid, 3.5 milligrams, riboflavin.
(2) testing index: weigh early morning on an empty stomach in the time of on-test and end, records feed consumption rate every day; After feeding experiment finishes, every group is repeated to select body weight and all approaches each 1 of average weight male and female chicken, wing vein Bian blood 3~5ml on an empty stomach in the morning, and the blood sample of collection is with the speed of 3000 revs/min after centrifugal ten minutes, separation of serum, sealing is stored in-20 DEG C of refrigerators to be measured.Blood sugar, triglycerides, T-CHOL, total serum protein, albumin, globulin, urea nitrogen are measured with automatic clinical chemistry analyzer.
(3) measurement result: pheasant raising of the present invention sees the following form 3 with auxotype growth promotion preparation to the impact of pheasant growth indexes.
Pheasant of the present invention is raised with auxotype growth promotion preparation as shown in table 4 below on the impact of pheasant blood parameters.
In table 3, table 4: indicate same letter with a line shoulder and represent not remarkable (P > 0.05) of difference, letter neighboar lists significant difference (P < 0.05), letter is separated by and is represented extremely significantly (P < 0.01) of difference.
By table 3, table 4 is visible, add 1.5% growth promotion preparation of the present invention, can only improve feed intake (P < 0.05), the growth promotion preparation of the present invention of interpolation 2% can obviously improve the weightening finish of pheasant, feed intake, material anharmonic ratio, blood sugar, total protein, the content (P < 0.05) of albumin and urea nitrogen, add 2.5% growth promotion preparation, also can improve the concentration (P < 0.05) of triglycerides, and further expand the advantage of weightening finish and material anharmonic ratio to difference level (P < 0.01) extremely significantly, add 6% growth promotion preparation, effect approaches with interpolation 2.5%, therefore the broiler diets of growth promotion preparation of the present invention is 2~2.5%.
Test 2: pheasant of the present invention is raised the impact on pheasant growth indexes and utilization of nutrients with auxotype growth promotion preparation.
(1) experimental design: select 300 of color pheasants in 8 week age seven, be divided at random 5 groups, establish 3 for every group and repeat 20 chickens of each repetition, male and female half and half.1 week preliminary trial period, just trying at the beginning of phase from the 9th week age of pheasant, finished to 16 week age Mo, control group is fed basal diet, 1~4 group of test group is fed respectively corresponding test daily ration, composition and the trophic level of respectively organizing daily ration see the following form 5.In experimental period, test pheasant free choice feeding and drinking-water, feeding and management and immune programme for children carry out according to normal feeding process.Test data is carried out statistical analysis with SPSS11.0 statistical software, when significant difference, carries out multiple ratio by SSR method, and test data represents by average ± standard deviation.
Wherein, compound premix provides 1.3 milligrams of lysines, 80 milligrams, manganese to every kilogram of daily ration, 60 milligrams, zinc, 6 milligrams of copper, 70 milligrams of iron, 0.2 milligram, selenium, 0.3 milligram of iodine, vitamin A 3 000 IU, neo dohyfral D3 1500 IU, vitamin E 30 IU, 3 milligrams of vitamin Ks, 70 milligrams, nicotinic acid, 10 milligrams, pantothenic acid, 3.5 milligrams, riboflavin.
(2) testing index: weigh early morning on an empty stomach in the time of on-test and end, records feed consumption rate every day; After feeding experiment finishes, enter metabolic test, amount to 5 days, adopt the conventional full excrement method of receiving, the ight soil of collecting is added 10% hydrochloric acid dry and pulverize in 65~70 DEG C.Organic matter is measured and is pressed GB/T6438-92 method, and energy is measured with oxygen bomb calorimeter, crude protein Kjeldahl's method, lysine and methionine are measured with amino acid fully-automatic analyzer, crude fibre acid-alkali treatment method, coarse ash calcination method, calcium EDTA method, phosphorus molybdenum yellow method.
Wherein: nutrient availability=((amount of nutrients of searching for food-fecaluria excretion amount of nutrients)/amount of nutrients of searching for food) × 100%
(3) measurement result: pheasant of the present invention is raised with auxotype growth promotion preparation as shown in table 6 below on the impact of pheasant growth indexes.
Pheasant of the present invention is raised with auxotype growth promotion preparation as shown in table 7 below on the impact of pheasant utilization of nutrients.
In table 6, table 7: indicate same letter with a line shoulder and represent not remarkable (P > 0.05) of difference, letter neighboar lists significant difference (P < 0.05), letter is separated by and is represented extremely significantly (P < 0.01) of difference.
By table 6, table 7 is visible, in the daily ration of seven color pheasants, add 1.5% growth promotion preparation, can only make the utilization rate of phosphorus be improved significantly (P < 0.05), add 2% growth promotion preparation, can make the weightening finish of pheasant, material anharmonic ratio, organic matter utilization rate, capacity usage ratio, crude protein utilization rate, methionine, lysine utilization rate, crude fibre utilization rate and calcium utilization significantly improve (P < 0.05), the utilization rate of phosphorus obtains the utmost point and significantly improves (P < 0.01), add 2.5% growth promotion preparation and can make material anharmonic ratio and the calcium utilization of pheasant further improve (P < 0.01), and growth promotion preparation is while adding 4% to, indices while being 2.5% with addition approaches (P < 0.05) very much, therefore the broiler diets of growth promotion preparation of the present invention is 2~2.5%.
From above-mentioned test 1, test 2, in feed, adding pheasant of the present invention raises with auxotype growth promotion preparation, can increase the feed intake of pheasant, obviously improve the speed of growth, the shortening growth cycle of pheasant, can effectively improve the utilization rate of various nutritional labelings in feed simultaneously, raise pheasant delicious meat, be of high nutritive value, there are wide market prospects.
The present invention is described in detail above in conjunction with the embodiments, but, person of ordinary skill in the field can understand, do not departing under the prerequisite of aim of the present invention, can also change each design parameter in above-described embodiment, form multiple specific embodiments, be common excursion of the present invention, describe in detail no longer one by one at this.

Claims (10)

1. a pheasant is raised with auxotype growth promotion preparation, it is characterized in that, in mass fraction, its raw materials comprises: 40~55 parts Chickpea Protein peptide, 20~25 parts of hydrolyzed wheat proteins, 15~25 parts of pea protein peptides, 0.5~1.0 part of complex enzyme, 0.2~0.4 part of phytase, 7~12 parts of cornstarch.
2. pheasant according to claim 1 is raised with auxotype growth promotion preparation, it is characterized in that, described complex enzyme is made up of zytase, dextranase, cellulase, acid protease, neutral proteinase, amylase, seminase, pectase.
3. pheasant according to claim 2 is raised with auxotype growth promotion preparation, it is characterized in that, in described complex enzyme, the enzyme amount of various enzymes is: zytase 18000~22000 U/g, dextranase 20000~30000U/g, cellulase 6000~7000U/g, acid protease 2500~3500U/g, neutral proteinase 1000~2000U/g, amylase 3000~4000U/g, seminase 3000~4000U/g, pectase 2000~3000U/g.
4. pheasant according to claim 1 is raised with auxotype growth promotion preparation, it is characterized in that, the enzyme amount of described phytase is 4500~5500U/g.
5. pheasant according to claim 1 is raised with auxotype growth promotion preparation, it is characterized in that, the addition of described growth promotion preparation is 1.5~6% of pheasant feeding quality.
6. pheasant according to claim 5 is raised with auxotype growth promotion preparation, it is characterized in that, the addition of described growth promotion preparation is 2~2.5% of pheasant feeding quality.
7. described in claim 1, pheasant is raised the preparation method with auxotype growth promotion preparation, it is characterized in that, comprises the following steps:
(1), in mass fraction, choose following raw materials: 40~55 parts Chickpea Protein peptide, 20~25 parts of hydrolyzed wheat proteins, 15~25 parts of pea protein peptides, 0.5~1.0 part of complex enzyme, 0.2~0.4 part of phytase, 7~12 parts of cornstarch;
(2) first complex enzyme, phytase are mixed with cornstarch;
(3) mixture of step (2) gained is mixed with remaining other component make pheasant auxotype growth promotion preparation again.
8. pheasant according to claim 7 is raised the preparation method with auxotype growth promotion preparation, it is characterized in that, the preparation method of described Chickpea Protein peptide is:
A. the Chickpea Protein powder suspension that is 8~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0, reacts 2.5~3 hours;
B. in the solution of steps A gained, add the HCl of 1mol/L to adjust its pH to 7.0, then add neutral enzymatic hydrolysis 1.5~2 hours by 0.3~0.5% of solution quality;
C. step B gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, being then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal being processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain Chickpea Protein peptide.
9. pheasant according to claim 7 is raised the preparation method with auxotype growth promotion preparation, it is characterized in that, the preparation method of described hydrolyzed wheat protein is:
A. the Gluten aaerosol solution that is 9~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0~8.3 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0~8.3, reacts 2.5 ~ 3 hours;
B. in the solution of step a gained, add the HCl of 1mol/L to adjust its pH to 7.0, then add food flavor enzyme hydrolysis 1.5~2 hours by 0.2~0.3% of solution quality;
C. step b gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, being then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal being processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain hydrolyzed wheat protein.
10. pheasant according to claim 7 is raised the preparation method with auxotype growth promotion preparation, it is characterized in that, the preparation method of described pea protein peptide is:
1. the Fed Protein Powder of Pea Insteal aaerosol solution that is 9~10% by mass fraction is placed in enzyme reactor, be heated to after 50 DEG C, adjust its pH to 8.0~8.3 with the sodium hydroxide solution of 1mol/L, then add alkali protease by 1% of its contained protein quality, the sodium hydroxide solution that drips 1mol/L in course of reaction makes its pH be stabilized in 8.0~8.3, reacts 2.5 ~ 3 hours;
2. 1. in the solution of gained, add the HCl of 1mol/L to adjust its pH to 7.0 to step, then add trypsin hydrolysis 1.5~2 hours by 0.2~0.3% of solution quality;
3. by step 2. gained reactant liquor be heated to boil and keep 5~10 minutes enzymes that go out, be then cooled to 25~30 DEG C, then with the speed of 2000 revs/min, this solution centrifugal processed 10 minutes, the supernatant of gained is sprayed and is drying to obtain hydrolyzed wheat protein.
CN201410230150.1A 2014-05-28 2014-05-28 Pheasant is raised with auxotype growth promotion preparation and preparation method thereof Expired - Fee Related CN103989018B (en)

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CN105029099A (en) * 2015-08-28 2015-11-11 河南牧业经济学院 Nutritional preparation capable of reducing death rate at later stage of growth of broiler chickens
CN105475668A (en) * 2015-12-18 2016-04-13 赵国中 Chinese herbal feed eaten by laying hens
CN105876154A (en) * 2016-04-22 2016-08-24 宜昌市昌伟饲料科技有限公司 Antibiotic-free feed for livestock and poultry and production method thereof
CN105995129A (en) * 2016-05-11 2016-10-12 郎溪县稻草帽生态畜禽养殖场 Pheasant nutrition promoter and preparation method thereof
CN106879860A (en) * 2016-12-31 2017-06-23 安徽宏亮饲料科技有限公司 A kind of feed addictive for preventing chicken to die suddenly

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CN105995129A (en) * 2016-05-11 2016-10-12 郎溪县稻草帽生态畜禽养殖场 Pheasant nutrition promoter and preparation method thereof
CN106879860A (en) * 2016-12-31 2017-06-23 安徽宏亮饲料科技有限公司 A kind of feed addictive for preventing chicken to die suddenly

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