CN105767507A - Feed micro-ecological preparation, anti-nutritional substance-free feed and preparation method thereof - Google Patents
Feed micro-ecological preparation, anti-nutritional substance-free feed and preparation method thereof Download PDFInfo
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Abstract
The present invention discloses a feed micro-ecological preparation, an anti-nutritional substance-free feed and a preparation method thereof, and belongs to the technical field of feed. The feed micro-ecological preparation is prepared by fermenting the following raw materials in parts by weight: 1.0-1.2 parts of Saccharomyces cerevisiae, 0.2-0.6 part of Enterococcus faecalis, 1.5-2.0 parts of Bacillus subtilis, 0.2-0.6 part of Bacillus coagulans, 0.1-0.15 part of Bacillus licheniformis, 0.1-0.3 part of Bacillus lentus, 0.2-0.5 part of Clostridium butyricum and 993.05-995.15 parts of liquid medium. After the feed micro-ecological preparation is mixed with feed raw materials, enzymes produced in the process of microbial strain fermentation can decompose anti-nutritional substances in feed and increase the feed utilization rate; at the same time, microbial strains also have effects in improving animal intestinal environment, maintaining intestinal flora balance, inhibiting the growth of pathogenic bacteria, and improving animal immunity.
Description
Technical field
The present invention relates to a kind of feedstuff microbial ecological agent, further relate to a kind of nonreactive feedstuff containing feedstuff microbial ecological agent and preparation method thereof, belong to field of feed.
Background technology
At present in poultry, aquaculture field, many employings are that full price coordinates granulated meal or full price to coordinate mash feed.Its primary raw material consists of the raw materials such as Semen Maydis, Semen Tritici aestivi, Sorghum vulgare Pers., Fructus Hordei Vulgaris, bean cake, cottonseed meal, rapeseed cake.But above raw material nutrition is single and contains substantial amounts of anti-nutrient substance, containing the antinutritional factor such as trypsin ihhibitor, phytohemagglutinin in Semen Maydis, bean pulp type feedstuff, its nutritional labeling is absorbed be greatly affected, and feedstuff there is also the carbohydrate such as beta-mannase, the alpha-galactoside etc. that are difficult to be digested by poultry, they are also the key factors affecting Semen Maydis, bean pulp type feed nutritive value so that the nutritional labeling in Semen Maydis, bean pulp type feedstuff is difficult to fully be digested and assimilated by poultry；Other feedstuffs also all contain anti-nutrient substance and toxin, the giucosinolate etc. in the araboxylan in Semen Tritici aestivi and side-product thereof, the beta glucan in Fructus Hordei Vulgaris, rapeseed cake.The existence of anti-nutrient substance not only reduces the utilization rate of feedstuff, and inhibits the growth of animal；In order to improve the nutrition of poultry, in addition it is also necessary to increase the consumption of feedstuff, add the cost of livestock and poultry cultivation, increase the Production of Livestock and Poultry demand pressure to feedstuff；Meanwhile, nutrient substance not digested in a large number excretes with feces residue, not only wastes the protein resource of preciousness, but also causes water and soil " eutrophication " to pollute.
The open a kind of meat-type duck mixed feed of prior art CN105146219A, following components is comprised: Semen Maydis 20-55% Semen Tritici aestivi 10-25% Sorghum vulgare Pers. 10-25% corn DDGS 10-15% soybean meal 5-10% guar meal 3-6% cottonseed meal 3-6% compound enzymic preparation 0.02-0.04% calcium hydrogen phosphate 0.5-10% Sal 0.2-0.3% soyabean lecithin oil 1-2% lysine 0.1-0.3% stone powder 0.5-1% methionine 0.04-0.06% threonine 0.02-0.05% microbial ecological agent 0.06-0.1% compound premix 1-2% with weight percentage raw material, this meat-type duck mixed feed comprehensive nutrition, absorption easy to digest.But the constituent species of this mixed feed is more, cause the high expensive of feedstuff；And the digestion of anti-nutrient substance is had some limitations by this feedstuff.Therefore, await exploitation a kind of with low cost, the nutrient substance in feedstuff can be fully absorbed, the nonreactive green feed of animal intestinal environment can be improved.
Summary of the invention
It is an object of the invention to provide a kind of feedstuff microbial ecological agent, this feedstuff Microecological bacteria activity is good, can produce multiple metabolic enzyme.
Second purpose of the present invention is to provide a kind of nonreactive feedstuff containing feedstuff microbial ecological agent；This nonreactive feedstuff has the effect improving the absorption of feedstuff Middle nutrition substance digestion, maintenance intestinal microbial population balance, promotion growth of animals or poultry.
The preparation method that 3rd purpose of the present invention is to provide a kind of nonreactive feedstuff containing feedstuff microbial ecological agent.
In order to realize object above, the technical solution adopted in the present invention is:
A kind of feedstuff microbial ecological agent, is made up of the fermenting raw materials of following parts by weight: saccharomyces cerevisiae 1.0～1.2 parts, enterococcus faecalis 0.2～0.6 part, bacillus subtilis 1.5～2.0 parts, Bacillus coagulans 0.2～0.6 part, Bacillus licheniformis 0.1～0.15 part, bacillus lentus 0.1～0.3 part, Clostridium butyricum 0.2～0.5 part, fluid medium 993.05～995.15 parts.
The viable count of described saccharomyces cerevisiae be 150～20,000,000,000 CFU/g, enterococcus faecalis viable count be 50～10,000,000,000 CFU/g, bacillus subtilis viable count be 200～30,000,000,000 CFU/g, Bacillus coagulans viable count be 100～20,000,000,000 CFU/g, Bacillus licheniformis viable count be 50～10,000,000,000 CFU/g, bacillus lentus viable count be 50～10,000,000,000 CFU/g, Clostridium butyricum viable count be 50～10,000,000,000 CFU/g.
Described fluid medium, is made up of the component of following parts by weight: 50～100 parts of brown sugar, vitamin B2 0.05～0.1 part, vitamin B12 0.02～0.05 part, yeast nucleic acid 5～9 parts, egg albumen powder 10～25 parts, 1～3 part of sodium chloride, potassium dihydrogen phosphate 1～2 part, sterile distilled water 803～864.93 parts.
The condition of fermentation is 35.5～37.5 DEG C of fermentation 48～72h；In sweat, first to culture fluid air-supply 24～36h, strain carries out aerobic fermentation, produces the metabolite such as ethanol, protease, cellulase in sweat；Rear 24～36h is out-of-blast, and strain carries out anaerobic fermentation, produces the metabolite such as lactic acid, antibacterial peptide in sweat.
A kind of nonreactive feedstuff containing above-mentioned feedstuff microbial ecological agent, is made up of the component of following parts by weight: feedstuff microbial ecological agent 225～275 parts, Semen Maydis 405～495 parts, Sorghum vulgare Pers. 135～200 parts, bean cake 90～150 parts, rapeseed cake 67.5～82.5 parts, cottonseed meal 76.5～93.5 parts, 90～110 parts of Testa Tritici, feed additive 36～44 parts.
A kind of preparation method of above-mentioned nonreactive feedstuff, it is characterised in that: comprise the following steps:
(1) take the Semen Maydis of formula ratio, Sorghum vulgare Pers., bean cake, rapeseed cake, cottonseed meal, Testa Tritici and feed additive, after pulverizing, mix to obtain forage mixture, standby；
(2) by the feedstuff microbial ecological agent of formula ratio and the mixing of step (1) gained forage mixture, 18～35 DEG C store 36～72h, obtain the nonreactive feedstuff containing feedstuff microbial ecological agent.
The strain of the feedstuff microbial ecological agent of the present invention contains saccharomyces cerevisiae, enterococcus faecalis, bacillus subtilis, Bacillus coagulans, Bacillus licheniformis, bacillus lentus and Clostridium butyricum, a large amount of enzyme material can be produced during the fermentation, such as xylanase, glucanase, mannonase cellulase, pectase, protease, amylase etc., the enzyme that microorganism produces can effectively divide the multiple anti-nutrient substance in feedstuff, improves the digestive utilization ratio of feed nutrient.It addition, Bacillus coagulans can also produce the tart flavour small-molecule substances such as a large amount of lactic acid and acetic acid at sweat, these small-molecule substances possess the sour fragrance of uniqueness, color and luster and mouthfeel etc., improve palatability；In saccharomycetic metabolite, there is possibly together with hormone, vitamin, nucleic acid etc. the biological active substances promoting animal growth；The organic molecule acid such as the lactic acid that the short-chain fatty acid such as the butanoic acid of Clostridium butyricum generation, acetic acid and Bacillus coagulans produce directly are absorbed in generating unit, can also regulating intestinal canal pH, stimulate intestinal peristalsis promoting, improve intestinal microenvironment, suppress the propagation of pathogen and regulating intestinal canal microecological balance, vitamin B group and vitamin K can also be produced, provide nutrition for animal；Bacillus subtilis can improve the activity of animal body internal interference element and macrophage, strengthens animal immunizing power；Bacillus licheniformis also can produce antibacterial substance, kills pathogenic bacterium；Enterococcus faecalis can suppress the breeding of putrefaction bacteria in intestinal, reduces intestinal endotoxin.
The present invention nonreactive feedstuff containing microbial ecological agent, make full use of the vital movement of microorganism and the effect of metabolite thereof, can not only degrade the antinutritional factor existed in feedstuff, improve feed nutrition utilization rate, also can supplement or recover the dominant microflora in intestinal, the Tiny ecosystem flora making imbalance reaches balance again, reduces antibiotic use；The generation of livestock and poultry animal ammonia nitrogen and other corrupt substances can also be reduced, reduce the generation of antibacterial ammonia in intestinal, reduce the excretion of nitrogen phosphorus, neutralize part toxin material so that livestock and poultry animal sickness rate is low；Meanwhile, the nonreactive feed cost containing feedstuff microbial ecological agent of the present invention is cheap, green non-pollution, is conducive to aquaculture to go on the road for development of economy type.
After the present invention nonreactive feedstuff feeding laying hen containing feedstuff microbial ecological agent, eggshell color can be improved, reduce broken egg soft egg rate, extend peak of laying eggs, after delaying peak period, improve the regularity of Growing Chicken；Broiler can improve dressing percentage, reduce death rate, improve survival rate, reduce intestinal coccidiosis incidence rate；Kind of chicken can be improved hatchable egg rate, incubation rate, improve strong young rate, improve laying rate and minimizing stress；In boar, feed intake and secrete milk amount in summer can be maintained, improve boar and produce performance；Also can improve market pig survival rate, reduce mortality, health invigorating, improve immunologic function, improve the infection of the diseases such as opposing high heat, foot and mouth disease, reproductive and respiratory syndrome.
The usage of the present invention nonreactive feedstuff containing feedstuff microbial ecological agent is: add 5～10% in brood feedstuff, adds 10%～20% in finishing period animal feed.
The preparation method of the present invention nonreactive feedstuff containing microbial ecological agent, by the feedstuff such as Semen Maydis, Sorghum vulgare Pers. with feedstuff with after microbial ecological agent mixed fermentation, drying can directly not use, and preparation technology is simple, easy to operate, is suitable for large-scale application and produces.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further described.The brown sugar adopted in implementation below is food stage；Vitamin B2 is feed grade, and effective content is 80%；Vitamin B12 is feed grade, and effective content is 2%；Yeast nucleic acid is food stage, and effective content is 8%；Egg albumen powder is food stage, protein content 80%；Potassium dihydrogen phosphate is food stage, potassium content 98%；Semen Maydis, one-level；Sorghum vulgare Pers.: one-level；Bean cake is protein content is the bean cake of 45%；Rapeseed cake is protein content is the rapeseed cake of 38%；Cottonseed meal are protein content is the cottonseed meal of 41%；Testa Tritici is large stretch of Testa Tritici.
Embodiment 1
The feedstuff microbial ecological agent of the present embodiment, is made up of the fermenting raw materials of following parts by weight: saccharomyces cerevisiae 1.0 parts, enterococcus faecalis 0.6 part, bacillus subtilis 1.5 parts, Bacillus coagulans 0.6 part, Bacillus licheniformis 0.15 part, bacillus lentus 0.3 part, Clostridium butyricum 0.5 part, fluid medium 995.15 parts.The condition of fermentation is that 36 DEG C of fermentations 60h, front 30h blow with the high pressure centrifugal fan of 1.5 kilowatts, and rear 30h is out-of-blast.
The viable count of described saccharomyces cerevisiae be 20,000,000,000 CFU/g, enterococcus faecalis viable count be 10,000,000,000 CFU/g, bacillus subtilis viable count be 20,000,000,000 CFU/g, Bacillus coagulans viable count be 10,000,000,000 CFU/g, Bacillus licheniformis viable count be 5,000,000,000 CFU/g, bacillus lentus viable count be 10,000,000,000 CFU/g, Clostridium butyricum viable count be 7,500,000,000 CFU/g.
Described fluid medium, is made up of the component of following parts by weight: 100 parts of brown sugar, vitamin B2 0.1 part, vitamin B12 0.05 part, yeast nucleic acid 9 parts, egg albumen powder 25 parts, 3 parts of sodium chloride, potassium dihydrogen phosphate 2 parts, sterile distilled water 816 parts.
The nonreactive feedstuff containing feedstuff microbial ecological agent of the present embodiment, is made up of the component of following parts by weight: feedstuff microbial ecological agent 250 parts, Semen Maydis 495 parts, Sorghum vulgare Pers. 135 parts, bean cake 110 parts, rapeseed cake 82.5 parts, cottonseed meal 85 parts, 90 parts of Testa Tritici, feed additive 36 parts.
The preparation method of the nonreactive feedstuff containing feedstuff microbial ecological agent of the present embodiment, comprises the following steps:
(1) take the Semen Maydis of formula ratio, Sorghum vulgare Pers., bean cake, rapeseed cake, cottonseed meal, Testa Tritici and feed additive, after pulverizing, mix to obtain forage mixture, standby；
(2) with fluid flowmeter by the feedstuff spraying micro-ecology preparation of formula ratio on step (1) gained forage mixture, after mixing, 27 DEG C store 72h, obtain the nonreactive feedstuff containing feedstuff microbial ecological agent.
Embodiment 2
The feedstuff microbial ecological agent of the present embodiment, is made up of the fermenting raw materials of following parts by weight: saccharomyces cerevisiae 1.2 parts, enterococcus faecalis 0.2 part, bacillus subtilis 2.0 parts, Bacillus coagulans 0.2 part, Bacillus licheniformis 0.1 part, bacillus lentus 0.3 part, Clostridium butyricum 0.2 part, fluid medium 993.05 parts.The condition of fermentation is that 36 DEG C of fermentations 72h, front 36h blow with the high pressure centrifugal fan of 1.5 kilowatts, and rear 36h is out-of-blast.
The viable count of described saccharomyces cerevisiae be 15,000,000,000 CFU/g, enterococcus faecalis viable count be 5,000,000,000 CFU/g, bacillus subtilis viable count be 30,000,000,000 CFU/g, Bacillus coagulans viable count be 15,000,000,000 CFU/g, Bacillus licheniformis viable count be 5,000,000,000 CFU/g, bacillus lentus viable count be 10,000,000,000 CFU/g, Clostridium butyricum viable count be 5,000,000,000 CFU/g.
Described fluid medium, is made up of the component of following parts by weight: 50 parts of brown sugar, vitamin B2 0.1 part, vitamin B12 0.02 part, yeast nucleic acid 5 parts, egg albumen powder 10 parts, 1 part of sodium chloride, potassium dihydrogen phosphate 2 parts, sterile distilled water 864.93 parts.
The nonreactive feedstuff containing feedstuff microbial ecological agent of the present embodiment, is made up of the component of following parts by weight: feedstuff microbial ecological agent 275 parts, Semen Maydis 405 parts, Sorghum vulgare Pers. 200 parts, bean cake 90 parts, rapeseed cake 67.5 parts, cottonseed meal 85 parts, 100 parts of Testa Tritici, feed additive 44 parts.
The preparation method of the nonreactive feedstuff containing feedstuff microbial ecological agent of the present embodiment, comprises the following steps:
(1) take the Semen Maydis of formula ratio, Sorghum vulgare Pers., bean cake, rapeseed cake, cottonseed meal, Testa Tritici and feed additive, after pulverizing, mix to obtain forage mixture, standby；
(2) with fluid flowmeter by the feedstuff spraying micro-ecology preparation of formula ratio on step (1) gained forage mixture, after mixing, 18 DEG C store 36, obtain the nonreactive feedstuff containing feedstuff microbial ecological agent.
Embodiment 3
The feedstuff microbial ecological agent of the present embodiment, is made up of the fermenting raw materials of following parts by weight: saccharomyces cerevisiae 1.1 parts, enterococcus faecalis 0.4 part, bacillus subtilis 2.0 parts, Bacillus coagulans 0.4 part, Bacillus licheniformis 0.17 part, bacillus lentus 0.2 part, Clostridium butyricum 0.2 part, fluid medium 993.05 parts.The condition of fermentation is that 37.5 DEG C of fermentations 60h, front 32h blow with the high pressure centrifugal fan of 1.5 kilowatts, and rear 28h is out-of-blast.
The viable count of described saccharomyces cerevisiae be 18,000,000,000 CFU/g, enterococcus faecalis viable count be 7,500,000,000 CFU/g, bacillus subtilis viable count be 30,000,000,000 CFU/g, Bacillus coagulans viable count be 20,000,000,000 CFU/g, Bacillus licheniformis viable count be 10,000,000,000 CFU/g, bacillus lentus viable count be 7,500,000,000 CFU/g, Clostridium butyricum viable count be 10,000,000,000 CFU/g.
Described fluid medium, is made up of the component of following parts by weight: 95 parts of brown sugar, vitamin B2 0.05 part, vitamin B12 0.03 part, yeast nucleic acid 7 parts, egg albumen powder 20 parts, 2 parts of sodium chloride, potassium dihydrogen phosphate 1 part, sterile distilled water 803 parts.
The nonreactive feedstuff containing feedstuff microbial ecological agent of the present embodiment, is made up of the component of following parts by weight: feedstuff microbial ecological agent 225 parts, Semen Maydis 450 parts, Sorghum vulgare Pers. 165 parts, bean cake 150 parts, rapeseed cake 75 parts, cottonseed meal 76.5 parts, 110 parts of Testa Tritici, feed additive 40 parts.
The preparation method of the nonreactive feedstuff containing feedstuff microbial ecological agent of the present embodiment, comprises the following steps:
(1) take the Semen Maydis of formula ratio, Sorghum vulgare Pers., bean cake, rapeseed cake, cottonseed meal, Testa Tritici and feed additive, after pulverizing, mix to obtain forage mixture, standby；
(2) with fluid flowmeter by the feedstuff spraying micro-ecology preparation of formula ratio on step (1) gained forage mixture, after mixing, 35 DEG C store 54h, obtain the nonreactive feedstuff containing feedstuff microbial ecological agent.
Embodiment 4
The feedstuff microbial ecological agent of the present embodiment, is made up of the fermenting raw materials of following parts by weight: saccharomyces cerevisiae 1.2 parts, enterococcus faecalis 0.4 part, bacillus subtilis 1.8 parts, Bacillus coagulans 0.6 part, Bacillus licheniformis 0.1 part, bacillus lentus 0.1 part, Clostridium butyricum 0.4 part, fluid medium 995.15 parts.The condition of fermentation is that 35.5 DEG C of fermentations 48h, front 24h blow with the high pressure centrifugal fan of 1.5 kilowatts, and rear 24h is out-of-blast.
The viable count of described saccharomyces cerevisiae be 20,000,000,000 CFU/g, enterococcus faecalis viable count be 7,500,000,000 CFU/g, bacillus subtilis viable count be 10,000,000,000 CFU/g, Bacillus coagulans viable count be 20,000,000,000 CFU/g, Bacillus licheniformis viable count be 7,500,000,000 CFU/g, the viable count of bacillus lentus be 5,000,000,000 CFU/g, Clostridium butyricum viable count be 10,000,000,000 CFU/g.
Described fluid medium, is made up of the component of following parts by weight: 100 parts of brown sugar, vitamin B2 0.07 part, vitamin B12 0.05 part, yeast nucleic acid 9 parts, egg albumen powder 25 parts, 3 parts of sodium chloride, potassium dihydrogen phosphate 1.5 parts, sterile distilled water 856.53 parts.
The nonreactive feedstuff containing feedstuff microbial ecological agent of the present embodiment, is made up of the component of following parts by weight: feedstuff microbial ecological agent 250 parts, Semen Maydis 450 parts, Sorghum vulgare Pers. 200 parts, bean cake 90 parts, rapeseed cake 75 parts, cottonseed meal 93.5 parts, 110 parts of Testa Tritici, feed additive 36 parts.
The preparation method of the nonreactive feedstuff containing feedstuff microbial ecological agent of the present embodiment, comprises the following steps:
(1) take the Semen Maydis of formula ratio, Sorghum vulgare Pers., bean cake, rapeseed cake, cottonseed meal, Testa Tritici and feed additive, after pulverizing, mix to obtain forage mixture, standby；
(2) with fluid flowmeter by the feedstuff spraying micro-ecology preparation of formula ratio on step (1) gained forage mixture, after mixing, 35 DEG C store 72h, obtain the nonreactive feedstuff containing feedstuff microbial ecological agent.
The nonreactive feedstuff containing feedstuff microbial ecological agent of embodiment of the present invention 1-4, in storage process, adopts electronic temperature control device to observe the change of material temperature, as material temperature must adopt cooling measure more than 45 DEG C；The shelf-life of the nonreactive feedstuff of gained feedstuff microbial ecological agent is: 3-6 month autumn and winter, 2-3 month spring and summer.
The usage of the embodiment of the present invention 1-4 gained nonreactive feedstuff containing feedstuff microbial ecological agent is: add 5～10% in brood feedstuff, adds 10%～20% in finishing period animal feed.
Experimental example 1
The ablactational baby pig (21 age in days) of 120 child care early stages is divided into 4 groups, respectively matched group and test group 1-3, often group 30；Matched group feeding chow diet, test group 1-3 feeding adds the feedstuff of the nonreactive feedstuff of the embodiment of the present invention 1, and adding proportion is 5%, 10%, 15%, raises 14 days, and culture experiment result is as shown in table 1:
Table 1 child care early stage ablactational baby pig culture experiment results contrast
As can be seen from Table 1, compared with matched group, add the present invention nonreactive feedstuff when being 5%-10%, the daily gain of child care early stage ablactational baby pig can be dramatically increased, reduce feedstuff-meat ratio, the energy of per kilogram of body weight converts and significantly reduces, per kilogram material energy is obviously improved, and addition is when reaching 15%, the daily gain increase amount of ablactational baby pig, feedstuff-meat ratio drop-out value, the energy of per kilogram of body weight converts decreasing value and per kilogram material energy lift value is all obvious not as when addition is 5-10%, show, when the nonreactive feedstuff proportion of the interpolation present invention is 5%-10%, the culture efficiency of child care early stage ablactational baby pig is good, cost is low.
Experimental example 2
The ablactational baby pig (36 age in days) in 120 child care later stages is divided into 4 groups, respectively matched group and test group 1-3, often group 30；Matched group feeding chow diet, test group 1-3 feeding adds the feedstuff of the embodiment of the present invention 2 nonreactive feedstuff, and adding proportion is 5%, 10%, 15%, raises 24 days, and culture experiment result is as shown in table 1:
Table 2 child care later stage ablactational baby pig culture experiment results contrast
From table 2 it can be seen that compared with matched group, when the nonreactive feedstuff of the interpolation present invention is 5%-10%, can dramatically increase the daily gain of child care later stage ablactational baby pig, reduce feedstuff-meat ratio, the energy of per kilogram of body weight converts and significantly reduces, and per kilogram material energy is obviously improved；And addition is when being 15%, little with culture efficiency difference when adding the amount of 5%-10%.Showing, when the nonreactive feedstuff proportion of the interpolation present invention is 5%-10%, the culture efficiency of child care later stage ablactational baby pig is good, and cost is low.
Experimental example 3
The ablactational baby pig (36 age in days) in 120 child care later stages is divided into 3 groups, respectively matched group and test group 1-2, often group 40；Matched group feeding chow diet, test group 1-2 feeding adds the feedstuff of the nonreactive feedstuff of the embodiment of the present invention 3, and adding proportion is 5%, 10%, and after 24 days, culture experiment result is as shown in table 3:
Table 3 culture experiment results contrast
| Group | Matched group | Test group 1 | Test group 2 |
| Metabolizable energy (Kcal/kg) | 3342 | 3342 | 3342 |
| Albumen/apparent digestible lysine | 18.15/1.15 | 18.15/1.15 | 18.15/1.15 |
| Add proportion (%) | 0 | 5 | 10 |
| Day feeding amount (kg/ head) | 0.757 | 0.770 | 0.787 |
From table 3 it can be seen that compared with matched group 2, when the nonreactive feedstuff of the interpolation present invention is 5%-10%, the Day feeding amount of piglet can be significantly improved, illustrate that the feed intake of the ablactational baby pig in child care later stage is improved effect by the nonreactive feedstuff of the present invention notable, good palatability.
Experimental example 4
The pig (61 age in days) of 152 head growth stages of fattening is divided into 4 groups, respectively matched group and test group 1-3, often group 38；Matched group feeding chow diet, test group 1-3 feeding adds the feedstuff of the nonreactive feedstuff of the embodiment of the present invention 4, and adding proportion is 10%, 20%, 30%, raises 49 days, and culture experiment result is as shown in table 3:
The pig culture experiment results contrast of table 4 growing period
From table 4, it can be seen that compared with matched group, when the nonreactive feedstuff of the interpolation present invention is 10%-20%, can significantly reduce feedstuff-meat ratio, the energy of per kilogram of body weight converts and significantly reduces, and per kilogram material energy is obviously improved；And addition is when being 30%, the adding weight of the pig of growing period, feedstuff-meat ratio, the energy conversion values of per kilogram of body weight and per kilogram material energy lift value with add proportion be 20% time, difference is inconspicuous.Considering cost, when the nonreactive feedstuff proportion of the interpolation present invention is 10%-20%, the growth performance increase rate of growing period pig is high, and culture efficiency is good.
Experimental example 5
The pig of 21 ages in days is divided into two groups: matched group and nonreactive feedstuff group, matched group feeding chow diet, nonreactive feedstuff group feeding adds the feedstuff of the nonreactive feedstuff of the embodiment of the present invention 1, until being fed to 152 ages in days, wherein, 21-35 age in days addition is 5%, and 36-60 age in days addition is 10%, 61-152 age in days addition is 20%, and result is in Table 5.
The each exponential case of table 5 compares
| Matched group | Nonreactive feedstuff group | P value | |
| Head number | 1832 | 2143 | - |
| Gross weight (kg) | 110.48 | 113.67 | - |
| Dressing percentage (%) | 76.66 | 78.11 | 0.06 |
| Back of the body fat (mm) | 17.75 | 17.42 | 0.005 |
| Muscle (mm) | 58.16 | 58.34 | 0.18 |
| Lean meat percentage (%) | 55.02 | 55.25 | 0.002 |
It is shown that compared with matched group, after adopting the nonreactive feedstuff feeding pig of the present invention, dressing percentage significantly improves, and back fat thickness significantly reduces, and lean meat percentage significantly raises；Showing that pig is high to the nonreactive feed nutrition absorption efficiency of the present invention, culture efficiency is good.
Experimental example 6
After the nonreactive feedstuff of the nursery pig feeding embodiment of the present invention 2 of 6.5～25kg, the diarrhea rate of pig, mortality rate experimental result in Table 6.
Table 6 results contrast
| Group | Diarrhea rate (%) | Mortality rate (%) |
| Matched group | 25.5 | 13.0 |
| Nonreactive feedstuff group | 1.5 | 0.5 |
Result shows: compared with matched group, and after adopting the nonreactive feedstuff feeding nursery pig of the present invention, the diarrhea rate of pig, mortality rate significantly reduce.
Claims (6)
1. a feedstuff microbial ecological agent, it is characterised in that: it is made up of the fermenting raw materials of following parts by weight: saccharomyces cerevisiae 1.0～1.2 parts, enterococcus faecalis 0.2～0.6 part, bacillus subtilis 1.5～2.0 parts, Bacillus coagulans 0.2～0.6 part, Bacillus licheniformis 0.1～0.15 part, bacillus lentus 0.1～0.3 part, Clostridium butyricum 0.2～0.5 part, fluid medium 993.05～995.15 parts.
2. feedstuff microbial ecological agent according to claim 1, it is characterised in that: the viable count of described saccharomyces cerevisiae be 150～20,000,000,000 CFU/g, enterococcus faecalis viable count be 50～10,000,000,000 CFU/g, bacillus subtilis viable count be 200～30,000,000,000 CFU/g, Bacillus coagulans viable count be 100～20,000,000,000 CFU/g, Bacillus licheniformis viable count be 50～10,000,000,000 CFU/g, bacillus lentus viable count be 50～10,000,000,000 CFU/g, Clostridium butyricum viable count be 50～10,000,000,000 CFU/g.
3. feedstuff microbial ecological agent according to claim 1, it is characterized in that: described fluid medium, be made up of the component of following parts by weight: 50～100 parts of brown sugar, vitamin B2 0.05～0.1 part, vitamin B12 0.02～0.05 part, yeast nucleic acid 5～9 parts, egg albumen powder 10～25 parts, 1～3 part of sodium chloride, potassium dihydrogen phosphate 1～2 part, sterile distilled water 803～864.93 parts.
4. feedstuff microbial ecological agent according to claim 1, it is characterised in that: the condition of fermentation is 35.5～37.5 DEG C of fermentation 48～72h.
5. the nonreactive feedstuff containing feedstuff microbial ecological agent as claimed in claim 1, it is characterised in that: it is made up of the component of following parts by weight: feedstuff microbial ecological agent 225～275 parts, Semen Maydis 405～495 parts, Sorghum vulgare Pers. 135～200 parts, bean cake 90～150 parts, rapeseed cake 67.5～82.5 parts, cottonseed meal 76.5～93.5 parts, 90～110 parts of Testa Tritici, feed additive 36～44 parts.
6. the preparation method of a nonreactive feedstuff as claimed in claim 5, it is characterised in that: comprise the following steps:
(1) take the Semen Maydis of formula ratio, Sorghum vulgare Pers., bean cake, rapeseed cake, cottonseed meal, Testa Tritici and feed additive, after pulverizing, mix to obtain forage mixture, standby；
(2) by the feedstuff microbial ecological agent of formula ratio and the mixing of step (1) gained forage mixture, 18～35 DEG C store 36～72h, obtain the nonreactive feedstuff containing feedstuff microbial ecological agent.
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