CN102067941B - Method for producing compound microbial preparation serving as fodder - Google Patents
Method for producing compound microbial preparation serving as fodder Download PDFInfo
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- CN102067941B CN102067941B CN2010105810666A CN201010581066A CN102067941B CN 102067941 B CN102067941 B CN 102067941B CN 2010105810666 A CN2010105810666 A CN 2010105810666A CN 201010581066 A CN201010581066 A CN 201010581066A CN 102067941 B CN102067941 B CN 102067941B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a method for producing a compound microbial preparation serving as fodder, which comprises the steps of microbial inoculum production, material proportioning, bacillus subtilis medium preparation, beer yeast medium preparation and lactobacillus plantarum medium preparation. According to the method, bacillus subtilis, beer yeast and lactobacillus plantarum are bred to form compound strains, so that the totality of the compound thalli is increased, and the advantages such as high strain survival rate, strong stability and the like are achieved; the compound thalli are mixed with ball-milling chaff, bran and oligoisomaltose in a proper proportion, and the optimum compounding proportion is formed for the preparation; good conditions are created for the formation of the internal environment of intestinal canals of livestock and poultry, the aerobic bacillus subtilis interacts with yeast to generate more nutrient substances, amylase, protease cellulose and the like; and meanwhile, oxygen in the intestinal canals of livestock and poultry can be consumed, excellent growth environment is created for propagation of lactic acid bacteria, intestinal canal functions of livestock and poultry are improved, the fodder conversion rate is improved, and the microecological balance of the intestinal canals of livestock and poultry is improved.
Description
Technical field
The present invention relates to a kind of preparation method of microorganism formulation, be specifically related to a kind of production method that adopts the feeding complex microorganism preparations of liquid deep layer fermenting.
Background technology
The digestive system of animal particularly has hundreds of in the intestines, tens billion of bacteriums forms intestinal flora, and it and animal health have confidential relation.Intestinal flora can also suppress the effect that pathogen propagation waits defence to infect in the various nutritional physiology effects of performance.The generation of the coliform disease of domestic animal, salmonella disease and clostruidium enteritis all with intestines in the state of bacterium substantial connection is arranged.Therefore, the balance of intestinal flora is kept normally to safeguarding that animal health is absolutely necessary.The raising and the improvement of each item index in it and the Production of Livestock and Poultry have substantial connection.
Microorganism formulation is the microbial product of one or more fungus strains in a kind of replacement or the balance animal ecology system.Say on the narrow sense that it is a kind ofly can excite the breeding of self probiotics to increase, and resists the microbial product of harmful fungus strain growth simultaneously.
Microorganism formulation contains multiple beneficial microorganisms such as a large amount of lactic acid bacterias, saccharomycete; After getting in the livestock and poultry body as feed, can breed rapidly, drop in the metabolin of bacterial classification on the one hand and enterogenous endotoxin; Suppressed the growth of other harmful flora; In host, formed normal microorganism species on the other hand,, nutrition and the invasion that stops pathogenic bacteria are provided for the synthetic main vitamin of host.
Microorganism formulation gets into and resists harmful bacterium in the intestines; Reduce the formation (stench is that the harmful substances such as toxicity amine, ammonia and hydrogen sulfide that produce during by harmful bacterium decomposing protein such as achalme's bacillus and amino acid cause) of stench and harmful components; Make the ight soil of discharge eliminate stench; Thereby alleviate the internal organs burden, make the peripheral vessel expansion, the supply of nutrition becomes smooth and easy.Simultaneously, owing to eliminated obstacle, cell proliferation is accelerated, and makes the meat, egg matter, the newborn matter that produce be improved.
Microorganism formulation product in the market mainly contains pulvis and aqua two big classes, but the general bacterial classification of powder product is single, and survival rate is low, poor stability.Aqua then transports inconvenience, and active storage life is short, easy autolyze phenomenon etc.
Summary of the invention
The object of the invention is to the deficiency of prior art; A kind of feeding complex microorganism preparations pulvis and manufacturing approach thereof are provided; The composite bacteria that complex microorganism preparations adopts bacillus subtilis, saccharomycete and lactobacillus to constitute; Solve defectives such as existing all kinds of microorganism formulation bacterial classifications are single, survival rate is low, poor stability, the short and easy autolyze of active storage life, increase substantially the output and the storage rate of microbial bacterial agent.
A kind of feeding complex microorganism preparations production method that provides of the present invention, this method may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, process pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis; Mix with ball milling chaff, wheat bran and oligoisomaltose; After weighing, packing, produce the feeding complex microorganism preparations of powder-type; Wherein, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 15～25%, wheat bran 74～84%, oligoisomaltose 0.02～1%, bacillus subtilis number>=2 * 10
7Cfu/g, saccharomyces cerevisiae number>=5 * 10
6Cfu/g and Lactobacillus plantarum number>=4.5 * 10
6Cfu/g.
Wherein, bacillus subtilis bacteria culture fluid of the present invention and bacillus subtilis cultural method are: glucose 5g, peptone 10g, beef extract 5g, sodium chloride 5g and water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature; Be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition; The bacillus subtilis slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, the rotating speed when shaking bottle work is 160rmp, 37 ℃ of temperature; Fermentation 24h, pH is controlled at 7.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 2.5～3m
3/ h, pH are 7.4, pressure 0.05～0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 15-20m
3/ h, pH are 7.4, pressure 0.05～0.1MPa, and with collecting through spray-drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, spray-dired formulation weight ratio is: maltodextrin 20, CaCl again
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 2-3kg/cm during spray-drying
2
Brewer's yeast nutrient solution of the present invention and saccharomyces cerevisiae cultural method are: yeast extract 5g, peptone 6g, glucose 20g and distilled water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Rotating speed 160rmp when shaking bottle work; 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change (temperature under 121 ℃, pressure 0.1MPa condition, sterilize 30min) the 50L seed fermentation jar of having sterilized, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 2.5～3m over to
3/ h, pH nature, pressure 0.05～0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 15～20m
3/ h, PH nature, pressure 0.05～0.1MPa.With collecting through freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-33～-35 ℃, drying time 18-20h.
Lactobacillus plantarum nutrient solution of the present invention and Lactobacillus plantarum cultural method are: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25g, epsom salt 0.58g, glucose 20g, Tween 80 1.0 mL and distilled water 1000 mL are packed in the lump shake in the bottle; 121 ℃ of temperature; Be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition; The Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.2～6.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 24h, pH6.2～6.4, pressure 0.05～0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of 500L fermentation jar temperatures, fermentation 72h, pH6.2～6.4, pressure 0.05～0.1MPa.With collecting through freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-33～-35 ℃, drying time 18-20h.
Moisture≤10.0% in the described feeding complex microorganism preparations, described pulvis are the composite bacteria agent capable of bacillus subtilis, brewer's yeast and Lactobacillus plantarum.
Change in order to investigate the inventive method sanitary index in process of production, the inventor counts content to the organoleptic indicator in the product, moisture, granularity, bacterial count of bacillus subtilis, saccharomyces cerevisiae number and Lactobacillus plantarum bacterium and measures by range estimation nasil (organoleptic indicator's determination method), GB/T 6435 (aquametry), GB/T 5917.1 (granulometry), bacillus subtilis colony counting method, brewer's yeast colony counting method and Lactobacillus plantarum colony counting method respectively.
Existing microorganism fodder preparation technique is compared on the present invention and the market, has the following advantages:
1, the present invention adopts the liquid deep layer fermenting technology, can obviously improve live body bacterium number in the finished product, and the fermentation fluid power reaches 1.0 * 10
10Cfu/mL, bacterium number>=2 * 10 of bacillus subtilis in the product
7Cfu/g, saccharomyces cerevisiae number>=5 * 10
6Cfu/g, Lactobacillus plantarum number>=4.5 * 10
6Cfu/g.
2, the present invention has improved the sum of thalline through improving fermentation batching and raise craft condition, has solved problems such as present bacterial classification is single, the viable count storage rate is low, and bacterial classification survival rate 1 year is up to 80%.
3, the present invention through seed selection, and through improving fermentation batching and raise craft condition, has improved the sum of composite thallus with bacillus subtilis, saccharomycete, three kinds of bacterial classifications of lactobacillus; Have bacterial classification survival rate, stability advantage such as strong; Mix with good proportioning with ball milling chaff, wheat bran and oligoisomaltose, make it form best compositely proportional, for good condition has been created in the formation of environment in its animal and bird intestines; Produce more multiple nutrient matter and amylase, protease cellulose etc. through aerobic bacteria bacillus subtilis and saccharomycete interaction; Can also exhaust simultaneously the oxygen in the animal and bird intestines, create good growing environment, improve the function of intestinal canal of livestock and poultry for the breeding of lactic acid bacteria; Improve food conversion ratio, improve and give birth to the animal and bird intestines microecological balance.
4, the inventive method is simple, and technical maturity is easy to grasp control, is convenient to promote.
The specific embodiment
Provide embodiment below so that the present invention is carried out concrete description; Be pointed out that following examples can not be interpreted as the restriction to protection domain of the present invention, the person skilled in the art of this area foregoing according to the present invention still belongs to protection scope of the present invention to some nonessential improvement and the adjustment that the present invention makes.
[embodiment 1]:The production method of feeding complex microorganism preparations, it may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, process pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis; Mix with ball milling chaff, wheat bran and oligoisomaltose; After weighing, packing, produce the feeding complex microorganism preparations of powder-type; In its product, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 15%, wheat bran 84%, oligoisomaltose 0.02%, wherein bacillus subtilis number>=2 * 10
7Cfu/g, saccharomyces cerevisiae number>=5 * 10
6Cfu/g and Lactobacillus plantarum number>=4.5 * 10
6Cfu/g.
Bacillus subtilis bacteria culture fluid and bacillus subtilis cultural method are: glucose 5.0g, peptone 10.0g, beef extract 5.0g, sodium chloride 5.0g and water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the bacillus subtilis slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Rotating speed when shaking bottle work is 160rmp; 37 ℃ of temperature, fermentation 24h, pH is controlled at 7.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 2.5m
3/ h, pH are 7.4, pressure 0.05MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 15m
3/ h, pH are 7.4, pressure 0.05MPa, and with collecting through spray-drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, spray-dired formulation weight ratio is: maltodextrin 20, CaCl again
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 2kg/cm during spray-drying
2
Brewer's yeast nutrient solution and saccharomyces cerevisiae cultural method are: yeast extract 5.0g, peptone 6.0g, glucose 20.0g and distilled water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Rotating speed 160rmp when shaking bottle work; 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 3m
3H, pH nature, pressure 0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 20m
3/ h; The pH nature, pressure 0.1MPa is with collecting through freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again; Cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-33 ℃, drying time 20h.
Lactobacillus plantarum nutrient solution and Lactobacillus plantarum cultural method are: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25g, epsom salt 0.58g, glucose 20g, Tween 80 1.0mL and distilled water 1000 mL are packed in the lump shake in the bottle; 121 ℃ of temperature; Be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition; The Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.2; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, and 37 ℃ of fermentation jar temperatures, fermentation 24h, pH 6.2, pressure 0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition; 37 ℃ of 500L fermentation jar temperatures, fermentation 72h, pH6.4; Pressure 0.1MPa; With collecting through freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-35 ℃, drying time 20h.
In the inventive method in the feeding complex microorganism preparations moisture be below 10.0%.
[embodiment 2]:The production method of feeding complex microorganism preparations is characterized in that: it may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, process pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis; Mix with ball milling chaff, wheat bran and oligoisomaltose; After weighing, packing, produce the feeding complex microorganism preparations of powder-type; In its product, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 25%, wheat bran 74%, oligoisomaltose 1%, wherein bacillus subtilis number>=2 * 10
7Cfu/g, saccharomyces cerevisiae number>=5 * 10
6Cfu/g and Lactobacillus plantarum number>=4.5 * 10
6Cfu/g.
Bacillus subtilis bacteria culture fluid and bacillus subtilis cultural method are: glucose 5.0g, peptone 10.0g, beef extract 5.0g, sodium chloride 5.0g and water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the bacillus subtilis slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Rotating speed when shaking bottle work is 160rmp; 37 ℃ of temperature, fermentation 24h, pH is controlled at 7.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 3m
3/ h, pH are 7.4, pressure 0.1MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 20m
3/ h, pH are 7.4, pressure 0.1MPa, and with collecting through spray-drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, spray-dired formulation weight ratio is: maltodextrin 20, CaCl again
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 3kg/cm during spray-drying
2。
Brewer's yeast nutrient solution and saccharomyces cerevisiae cultural method are: yeast extract 5.0g, peptone 6.0g, glucose 20.0g and distilled water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Rotating speed 160rmp when shaking bottle work; 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 2.5m
3/ h, pH nature, pressure 0.05MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 15m
3/ h; The pH nature, pressure 0.05MPa is with collecting through freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again; Cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-35 ℃, drying time 18h.
Lactobacillus plantarum nutrient solution and Lactobacillus plantarum cultural method are: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25.0g, epsom salt 0.58g, glucose 20.0g, Tween 80 1.0mL and distilled water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature; Be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition; The Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 24h, pH6.4, pressure 0.05MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition; 37 ℃ of fermentation jar temperatures, fermentation 72h, pH 6.2; Pressure 0.05MPa; With collecting through freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-33 ℃, drying time 18h.
In the inventive method in the feeding complex microorganism preparations moisture be below 10.0%.
[embodiment 3]:The production method of feeding complex microorganism preparations, it may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, process pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis; Mix with ball milling chaff, wheat bran and oligoisomaltose; After weighing, packing, produce the feeding complex microorganism preparations of powder-type; In its product, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 20%, wheat bran 79.5%, oligoisomaltose 0.5%, wherein bacillus subtilis number>=2 * 10
7Cfu/g, saccharomyces cerevisiae number>=5 * 10
6Cfu/g and Lactobacillus plantarum number>=4.5 * 10
6Cfu/g.
Bacillus subtilis bacteria culture fluid and bacillus subtilis cultural method are: glucose 5.0g, peptone 10.0g, beef extract 5.0g, sodium chloride 5.0g and water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature, be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Rotating speed when shaking bottle work is 160rmp; 37 ℃ of temperature, fermentation 24h, pH is controlled at 7.4; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 2.8m
3/ h, pH are 7.4, pressure 0.08MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 18m
3/ h, pH are 7.4, pressure 0.08MPa, and with collecting through spray-drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, spray-dired formulation weight ratio is: maltodextrin 20, CaCl again
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 2.5kg/cm during spray-drying
2
Brewer's yeast nutrient solution and saccharomyces cerevisiae cultural method are: yeast extract 5.0g, peptone 6.0g, glucose 20.0g and distilled water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature, be cooled to about 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Rotating speed 160rmp when shaking bottle work; 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 2.8m
3/ h, pH nature, pressure 0.08MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 18m
3/ h; The pH nature, pressure 0.08MPa is with collecting through freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting again; Cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-34 ℃, drying time 19h.
Lactobacillus plantarum nutrient solution and Lactobacillus plantarum cultural method are: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25g, epsom salt 0.58g, glucose 20.0g, Tween 80 1.0mL and distilled water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature; Be cooled to about 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition; The Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.3; And then change (30min sterilizes) the 50L seed fermentation jar of having sterilized under 121 ℃ of temperature, pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 24h, pH6.3, pressure 0.08MPa; Then change (30min sterilizes) the 500L fermentation tank of having sterilized again under 121 ℃ of temperature, pressure 0.1MPa condition; 37 ℃ of 500L fermentation jar temperatures, fermentation 72h, pH6.3; Pressure 0.08MPa; With collecting through freeze drying behind nutrient solution universal high speed tube centrifuge (rotating speed 16000rmp) the enrichment thalline after fermenting, cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying again
1MPa, 45 ℃ of baffle temperature, compressor temperature-34 ℃, drying time 19h.
In the inventive method in the feeding complex microorganism preparations moisture be below 10.0%.
Claims (1)
1. the production method of a feeding complex microorganism preparations, it is characterized in that: it may further comprise the steps:
(1) bacillus subtilis bacterial classification, brewer's yeast bacterial classification and Lactobacillus plantarum kind are inoculated in respectively in bacillus subtilis bacteria culture fluid, brewer's yeast nutrient solution and the Lactobacillus plantarum nutrient solution, process pulvis after collecting thalline respectively;
(2) the bacillus subtilis bacteria powder that step (1) is made, brewer's yeast bacteria powder and Lactobacillus plantarum pulvis; Mix with ball milling chaff, wheat bran and oligoisomaltose; After weighing, packing, produce the feeding complex microorganism preparations of powder-type; Wherein, the percentage by weight of each component is in the described feeding complex microorganism preparations: ball milling chaff 15～25%, wheat bran 74～84%, oligoisomaltose 0.02～1%, wherein bacillus subtilis number>=2 * 10
7Cfu/g, saccharomyces cerevisiae number>=5 * 10
6Cfu/g and Lactobacillus plantarum number>=4.5 * 10
6Cfu/g, moisture≤10.0%;
Wherein, described bacillus subtilis bacteria culture fluid and bacillus subtilis cultural method are: glucose 5g, peptone 10g, beef extract 5g, sodium chloride 5g and water 1000mL are packed in the lump shake in the bottle, 121 ℃ of temperature; Be cooled to 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition; The bacillus subtilis slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform, the rotating speed when shaking bottle work is 160rmp, 37 ℃ of temperature; Fermentation 24h, pH is controlled at 7.4; And then change 121 ℃ of temperature the 50L seed fermentation jar of sterilization 30min under the pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 2.5～3m over to
3/ h, pH are 7.4, pressure 0.05～0.1MPa; Then change temperature again over to, the 500L fermentation tank of sterilization 30min under the pressure 0.1MPa condition, fermentation tank rotating speed 160rmp, 37 ℃ of temperature, fermentation 24h, air quantity 15-20m at 121 ℃
3/ h, pH are 7.4, pressure 0.05～0.1MPa, and with collecting through spray-drying behind the nutrient solution universal high speed tube centrifuge enrichment thalline after fermenting, spray-dired formulation weight ratio is: maltodextrin 20, CaCl again
21, thalline 10,170 ℃ of inlet temperatures, 55 ℃ of outlet temperatures, pressure 2-3kg/cm during spray-drying
2
Described brewer's yeast nutrient solution and saccharomyces cerevisiae cultural method are: yeast extract 5g, peptone 6g, glucose 20g and distilled water 1000mL are packed in the lump shake in the bottle; 121 ℃ of temperature, be cooled to 30 ℃ behind the sterilization 20min under the pressure 0.1MPa condition, the brewer's yeast slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Rotating speed 160rmp when shaking bottle work; 30 ℃ of temperature, fermentation 24h, pH keeps nature; And then change 121 ℃ of temperature the 50L seed fermentation jar of sterilization 30min under the pressure 0.1MPa condition, the rotating speed 160rmp of fermentation tank, 30 ℃ of temperature, fermentation 24h, air quantity 2.5～3m over to
3/ h, pH nature, pressure 0.05～0.1MPa; Then change the 500L fermentation tank of sterilization 30min under the pressure 0.1MPa condition, the rotating speed 160rmp of 500L fermentation tank, 30 ℃ of temperature, fermentation 48h, air quantity 15～20m again over to 121 ℃ of temperature
3/ h; The PH nature, pressure 0.05～0.1MPa is with collecting through freeze drying behind the nutrient solution universal high speed tube centrifuge enrichment thalline after fermenting again; Cryodesiccated formulation weight ratio is: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-33～-35 ℃, drying time 18-20h;
Described Lactobacillus plantarum nutrient solution and Lactobacillus plantarum cultural method are: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, Sodium acetate trihydrate 5.0g, four water manganese sulfate 0.25g, epsom salt 0.58g, glucose 20g, Tween 80 1.0 mL and distilled water 1000 mL are packed in the lump shake in the bottle; 121 ℃ of temperature; Be cooled to 37 ℃ behind the sterilization 20min under the pressure 0.1MPa condition; The Lactobacillus plantarum slant strains of preserving is inoculated in good the shaking in the bottle of sterilization on aseptic platform; Shake 37 ℃ of bottle operating temperatures, fermentation 24h, pH is adjusted to 6.2～6.4; And then change 121 ℃ of temperature the 50L seed fermentation jar of sterilization 30min under the pressure 0.1MPa condition, 37 ℃ of fermentation jar temperatures, fermentation 24h, pH6.2～6.4, pressure 0.05～0.1MPa over to; Then change the 500L fermentation tank of sterilization 30min under the pressure 0.1MPa condition, 37 ℃ of 500L fermentation jar temperatures again over to 121 ℃ of temperature; Fermentation 72h; PH6.2～6.4, pressure 0.05～0.1MPa is with collecting through freeze drying behind the nutrient solution universal high speed tube centrifuge enrichment thalline after fermenting again; Cryodesiccated formula rate: trehalose 10, milk powder 10, thalline 5, vacuum 1.29 * 10 during freeze drying
1MPa, 45 ℃ of baffle temperature, compressor temperature-33～-35 ℃, drying time 18-20h;
The turn up 16000rmp of described high speed tube centrifuge, effectively separating thallus reaches more than 99%.
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| CN104381686A (en) * | 2014-11-24 | 2015-03-04 | 上海创博生态工程有限公司 | Spent mushroom substrate microbial milk-cow feed and preparation method thereof |
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| CN108391758A (en) * | 2018-04-25 | 2018-08-14 | 广西康佳龙农牧集团有限公司 | Milking sow compound Synbiotics |
| CN113881600A (en) * | 2021-10-29 | 2022-01-04 | 内蒙古自治区农牧业科学院 | Liquid culture medium for simultaneously culturing two different bacilli and culture method thereof |
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| CN101530165A (en) * | 2009-04-23 | 2009-09-16 | 上海谱莱生物技术有限公司 | Animal feed composite microecologic agent |
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