CN103518980B - Preparation method for microecologics for laying hen feeds and application of microecologics - Google Patents
Preparation method for microecologics for laying hen feeds and application of microecologics Download PDFInfo
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Abstract
The invention discloses a preparation method for microecologics for laying hen feeds and application of the microecologics. The microecologics is prepared by uniformly mixing living microcapsules bacterium powder with selenium carrageenan, zinc methionine and auxiliary materials through a spray drying technology, wherein living microcapsules bacterium powder is obtained by uniformly mixing lactobacillus plantarum and bacillus subtilis strains with an aqueous solution containing pectin, tween-80, glycerinum, mannitol, trehalose and sodium bicarbonate after the lactobacillus plantarum and the bacillus subtilis strains are subjected to enrichment culture. The microecologics can be stored under a normal-temperature condition and is high in enteric solubility and biological adhesive. On the basis of normal daily ration, the laying hens are also fed with the microecologics, so that the anti-oxidization capability of organ bodies of laying hens can be improved; therefore, the damage degree of fallopian tubes of the laying hens in the later period of laying is reduced, excessive release of inflammatory factors in tissues can be suppressed, and the injury caused by inflammatory reaction can be alleviated; the activity and the completeness of organ tissues and cells can be guaranteed, the organ health is retained, and the productivity is improved.
Description
Technical field
The present invention is specifically related to a kind of laying hen feeding micro-ecological preparation and preparation method thereof, belongs to probiotics field.
Background technology
Egg is the important sources that the mankind obtain protein, dark liking by domestic and international consumer.For a long time, layer breeding person is for improving egg output, realize maximization of economic benefit, generally adopt highdensity cage culture feeding mode, make laying hen lack motion, Immunoresistance declines, stress increase, and the energy of daily ration, protein ratio are uneven, laying hen histoorgan generation pathologic is very easily made to change.And the health of laying hen is its performance production performance, realizes the basic of maximizing the benefits.
Correlative study shows; often peroxidation can be there is in Egg Production of Laying Hens process; as lipid peroxidation destroys the complete of membrane structure and function, distress stimulates body to produce a large amount of active oxygens, causes the oxidative damage etc. of lipid peroxidation, protein and DNA.Simultaneously inflammatory reaction be also opening egg-layers clinical in common a kind of pathological change, all can occur at the tissue of body different parts and organ, especially occur the most common with positions such as fallopian tubals.The excessive release of Correlative Inflammatory Factors can cause histiocytic damage, and then destroys the integrality of respective organization organ, makes it to play its normal physiological function, has a strong impact on the health of laying hen, and reduce its production performance.
Problems existing in producing for above-mentioned laying hen, at present both at home and abroad all without good solution.
Summary of the invention
The present invention is in order to control an above-mentioned difficult problem for existence in laying hen production, the oxidation resistance having and improve laying hen is separated in healthy poultry intestinal tract, suppress the excessive release of inflammatory factor in tissue, reduce inflammation the probio bacterial classification reacted, efficient active bacteria formulation is processed into by composite, reach and effectively safeguard that laying hen is healthy and safe, improve the object of production performance.The object of this invention is to provide a kind of preparation method and application thereof of laying hen feeding micro-ecological preparation, for improving the oxidation resistance of laying hen, the fallopian tubal extent of damage in the later stage that makes to lay eggs reduces, and improves production performance; For suppressing the excessive release of Correlative Inflammatory Factors in histocyte, reduce inflammation reaction, safeguards that laying hen is healthy, improve the quality and yield of poultry product.
The object of the invention is to be achieved through the following technical solutions, a kind of preparation method of laying hen feeding micro-ecological preparation, comprises the following steps:
(1) Lactobacillus plantarum and Bacillus subtilis strain are mixed respectively after fermentation tank Zengjing Granule, obtain mixed bacteria liquid;
(2) described mixed bacteria liquid is fully mixed by same volume with the aqueous solution containing pectin, Tween 80, glycerine, sweet mellow wine, trehalose and sodium acid carbonate, after spray-dried, make microencapsulation viable bacteria powder; In the described aqueous solution, the mass percent of pectin, Tween 80, glycerine, sweet mellow wine, trehalose, sodium acid carbonate is respectively 0.5-1%, 0.8-1.5%, 0.8-1.2%, 0.5-1%, 3-6%, 2-3.5%;
(3) by described microencapsulation viable bacteria powder and selenide of carragheen, zinc methionine and auxiliary material Homogeneous phase mixing, obtained laying hen feeding micro-ecological preparation.
Fowl lactobacillu plantarurn CCTCC 2012079(LP1002), fowl source bacillus subtilis CCTCC 2012078(BS0903), be from the some probiotics bacterial classifications collected over the years, preserve, obtain after repeated screening for many years.Fowl lactobacillu plantarurn CCTCC 2012079(LP1002) on March 14th, 2012 send China typical culture collection center (China. Wuhan. Wuhan University) preservation, title and diagnostic characteristics: Lactobacillus plantarum (
lactobacillus plantarum), strain number: LP1002, deposit number CCTCC NO:M2012079; Fowl source bacillus subtilis CCTCC 2012078(BS0903) on March 14th, 2012 send China typical culture collection center (China. Wuhan. Wuhan University) preservation, title and diagnostic characteristics: bacillus subtilis (
bacillus subtilis), strain number: BS0903, deposit number CCTCC NO:M2012078.
The number that every gram of described laying hen feeding micro-ecological preparation contains Lactobacillus plantarum is 2.5-5 hundred million.
The number that every gram of described laying hen feeding micro-ecological preparation contains bacillus subtilis is 10-25 hundred million.
In described laying hen feeding micro-ecological preparation, the mass percent of selenide of carragheen is 0.05-0.12%.
In described laying hen feeding micro-ecological preparation, the mass percent of zinc methionine is 0.15-0.3%.
Described in step (3), auxiliary material is glucose.
The application of above-mentioned laying hen feeding micro-ecological preparation, by described laying hen feeding micro-ecological preparation in 1% ratio add in laying hens essential ration, feed to opening egg-layers after fully stirring.
Compared with prior art, the present invention has following beneficial effect:
The first, the fowl lactobacillu plantarurn that this probiotics adopts and bacillus subtilis are poultry probio, have and suppress immune response, the enhancing resisting stress capability of growth of pathogenic bacteria, stimulation body and provide the effects such as nutrition for body.In addition, bacillus subtilis also there is prevention and therapy acute dysentery, reduce cholesterol, radioresistance and the prebiotic activity such as anti-oxidant.
Second, this probiotics adopts pectin, sweet mellow wine, trehalose, sodium acid carbonate etc. as capsulating material, utilizes microcapsules technology to enable obtained active bacteria formulation keep its biologically active, and more stable, can store at normal temperatures, improve the utilization rate of resource simultaneously.In addition, select sodium acid carbonate as coating agent, can stomach inner pH value be improved simultaneously, promote digest and assimilate and improve the normal environment of stomach intestinal microbial; Also have and suppress or murder pathogenic entero becteria, propagation probio, improves microorganism species in enteron aisle, promotes growth of animal.Above-mentioned capsulating material has good biocompatibility and bioadhesive, and acceptor intrinsic factor does not affect, has the advantages such as controlled release fertilizer.Probiotics have employed sodium acid carbonate and pectin as enteric solubility capsulating material and trehalose and sweet mellow wine as heat-resistant stable agent, effectively improves activity and the stability of probio.
3rd, probiotics of the present invention adopts selenide of carragheen as selenium source.Research finds that inorganic selenium (as sodium selenite) has that peroxidating characteristic, toxicity are comparatively large and bioavailability is lower, harmful effect can be caused to animal and environment, and the activity of biogenic Organic Selenium exceedes inorganic selenium, and the absorption efficiency of animal gastrointestinal tract to Organic Selenium is higher than inorganic selenium.
4th, Organic Selenium and organic zinc and probio are kept apart by coating technique by probiotics of the present invention in the preparation, can make selenium, zinc both can be used as prebiotics and matched with probio after entering body, directly can be absorbed by body again, play its effect.
5th, find with application by experiment, probiotics of the present invention is applied in feed addictive of layer chicken, effectively can improve the oxidation resistance of laying hen, the fallopian tubal extent of damage of later stage laying hen of making to lay eggs reduces, and suppress the excessive release of inflammatory cytokine in tissue, reduce inflammation the damage of reacting and causing simultaneously, thus ensure vigor and the integrality of tissue and cell, safeguard the health of laying hen and improve production performance.
6th, the prebiotic substance that probiotics of the present invention contains not only can improve the oxidation resistance of laying hen, make later stage fallopian tubal extent of damage reduction of laying eggs, the excessive release organizing interior inflammatory factor can also be suppressed, reduce inflammation the damage of reacting and causing, thus ensure vigor and the integrality of body tissue and cell, maintain body health and improve production performance.
7th, probiotics of the present invention can store under normal temperature condition, and product has good enteric solubility and bioadhesive.Laying hen is on the basis of feeding normal daily ration, add this probiotics, not only can improve the oxidation resistance of body, the fallopian tubal extent of damage of later stage laying hen of making to lay eggs reduces, the excessive release organizing interior inflammatory factor can also be suppressed, reduce inflammation the damage of reacting and causing, thus ensure vigor and the integrality of body tissue and cell, maintains body health and improve production performance.
Accompanying drawing explanation
Fig. 1 be embodiment 3 stop feed probiotics within 15 days, respectively organize IL-1 β to express on later stage laying hen batterylaying of laying eggs affect comparison diagram;
Fig. 2 be embodiment 3 stop feed probiotics within 15 days, respectively organize IL-2 to express on later stage laying hen batterylaying of laying eggs affect comparison diagram;
Fig. 3 is that embodiment 3 is stopped feeding probiotics 15 days and affected comparison diagram to each tissue T NF-alpha expression of later stage laying hen batterylaying of laying eggs;
Fig. 4 be embodiment 3 stop feed probiotics within 45 days, respectively organize IL-1 β to express on later stage laying hen batterylaying of laying eggs affect comparison diagram;
Fig. 5 be embodiment 3 stop feed probiotics within 45 days, respectively organize IL-2 to express on later stage laying hen batterylaying of laying eggs affect comparison diagram;
Fig. 6 be embodiment 3 stop feed probiotics within 45 days, organize IL-4 to express on later stage laying hen batterylaying of laying eggs affect comparison diagram;
Fig. 7 is that embodiment 3 is stopped feeding probiotics 45 days and affected comparison diagram to later stage laying hen batterylaying tissue T NF-alpha expression of laying eggs;
Fig. 8 be embodiment 3 stop feed probiotics within 45 days, organize INF-r to express on later stage laying hen batterylaying of laying eggs affect comparison diagram;
Fig. 9 is the microencapsulation viable bacteria powder stereoscan photograph figure that the present invention obtains.
Detailed description of the invention
Embodiment 1
A preparation method for laying hen feeding micro-ecological preparation, comprises the following steps:
(1) Lactobacillus plantarum and Bacillus subtilis strain are mixed respectively after fermentation tank Zengjing Granule, obtain mixed bacteria liquid;
(2) described mixed bacteria liquid is fully mixed by same volume with the aqueous solution containing pectin, Tween 80, glycerine, sweet mellow wine, trehalose and sodium acid carbonate, after spray-dried, make microencapsulation viable bacteria powder (see figure 9); In the described aqueous solution, the mass percent of pectin, Tween 80, glycerine, sweet mellow wine, trehalose, sodium acid carbonate is respectively 0.5-1%, 0.8-1.5%, 0.8-1.2%, 0.5-1%, 3-6%, 2-3.5%;
(3) by described microencapsulation viable bacteria powder and selenide of carragheen, zinc methionine and auxiliary material Homogeneous phase mixing, obtained laying hen feeding micro-ecological preparation.
The number that every gram of described laying hen feeding micro-ecological preparation contains Lactobacillus plantarum is 2.5-5 hundred million.
The number that every gram of described laying hen feeding micro-ecological preparation contains bacillus subtilis is 10-25 hundred million.
In described laying hen feeding micro-ecological preparation, the mass percent of selenide of carragheen is 0.05-0.12%.
In described laying hen feeding micro-ecological preparation, the mass percent of zinc methionine is 0.15-0.3%.
Described in step (3), auxiliary material is glucose.
The application of above-mentioned laying hen feeding micro-ecological preparation, by described laying hen feeding micro-ecological preparation in 1% ratio add in laying hens essential ration, feed to opening egg-layers after fully stirring.
Embodiment 2
After the liquid fermentate of the Lactobacillus plantarum after fermentation tank culture and bacillus subtilis is mixed in proportion, after fully mixing by same volume with the solution containing 0.5% pectin, 0.8% Tween 80,0.8% glycerine, 0.5% sweet mellow wine, 6% trehalose and 3.5% sodium acid carbonate, viable bacteria powder is made after spray-dried, again bacterium powder is mixed with selenide of carragheen, zinc methionine and glucose, obtain laying hen feeding micro-ecological preparation.Show after detection, every gram of probiotics contains Lactobacillus plantarum 2.5 hundred million, bacillus subtilis 1,000,000,000, and the content of selenide of carragheen and zinc methionine is respectively 0.5% and 3%.By the probiotics prepared in 1% ratio add in basal diet, feed to opening egg-layers after fully stirring.
Experiment chooses two opening egg-layers of raising in cages, 10000 every, and be divided into control group (C) and experimental group (E), two groups are added probiotics on the basis of normal daily ration of feeding.During 450 age in days, C group stops feeding probiotics.Stop feeding 15, two groups of random selecting ten respectively after 45d, put to death and collect the mensuration that the sample such as blood and organ carries out index of correlation.
MDA and MDA, it is the primary product of peroxidatic reaction of lipid, the mensuration of MDA usually cooperatively interacts with the mensuration of SOD, the height indirect reaction of the SOD vigor ability of body scavenging activated oxygen, and the order of severity that the height indirect reaction of MDA body cell is attacked by free radical.SOD(superoxide dismutase) vital effect is played to the oxidative and anti-oxidative balance of body, it can remove ultra-oxygen anion free radical, and Cell protection escapes injury.
TAC (T-AOC) is the overall objective evaluated antioxidant ability of organism.Power and the health degree of the oxidation resistance of body defenses system also exist close ties, this defense system has enzymatic and non-enzymatic two individual system, many enzymes take trace element as activated centre, such as: superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), glutathione S-transferase (GST) etc., be mainly vitamin, amino acid and metalloprotein in non-enzymatic reaction system.Such as: V
e, carrotene, V
c, cysteine, methionine, tryptophan, histidine, glucose, ketone blue albumen, transferrins, lactoferrin etc.For anti-oxidant, above-mentioned system by eliminating free radical and active oxygen in order to avoid cause lipid peroxidation; And decompose hydroperoxide, block peroxidating chain; Removed the metal ion of catalytic action simultaneously.
Carry out oxidation resistance detection to 15 days two groups of Laying-hen Serums and each tissue, result is as shown in the table:
Table 1 stops feeding probiotics 15 days to laying eggs later stage Laying-hen Serum and organize the impact of oxidation resistance
Note: 1. in table, data all represent with mean value ± standard error;
2. * represents that E and C compares, P<0.05 (n=10);
3. * * represents that E and C compares, P<0.01 (n=10).
As shown in table 1, research finds, compared with C group, E group Laying-hen Serum SOD, T-AOC vigor and MDA content all do not have marked change (P>0.05); E group laying hen respectively organize in SOD, T-AOC vigor all on the rise, the active pole of fallopian tubal SOD, T-AOC is significantly higher than Normal group (P<0.05); MDA content has downward trend in heart, kidney, fallopian tubal, but is significantly higher than Normal group (P<0.05) in liver.Result shows when stopping feeding probiotics 15 days, and on laying eggs, later stage laying hen fallopian tubal impact is comparatively serious, significantly reduces its antioxidation activity; The adverse consequences that liver occurs infer with stop feeding time or liver compensatory movable strengthen relevant.
Carry out oxidation resistance detection to 45 days two groups of Laying-hen Serums and each tissue, result is as shown in the table:
Table 2 stops feeding probiotics 45 days to laying eggs later stage Laying-hen Serum and organize the impact of oxidation resistance
Note: 1. in table, data all represent with mean value ± standard error;
2. * represents that E and C compares, P<0.05 (n=10);
3. * * represents that E and C compares, P<0.01 (n=10).
As shown in table 2, research finds, compared with C group, E group Laying-hen Serum SOD, T-AOC vigor and MDA content all do not have marked change (P>0.05); E group laying hen respectively organize in T-AOC vigor on the rise, fallopian tubal, duodenum T-AOC activity are significantly higher than Normal group (P<0.05); MDA content has downward trend and remarkable (P<0.05) in heart, liver, duodenum.Result shows when stopping feeding probiotics 45 days, comparatively serious on later stage laying hen fallopian tubal of laying eggs, duodenum impact, significantly reduces its antioxidation activity.
Embodiment 3
After the liquid fermentate of the Lactobacillus plantarum after fermentation tank culture and bacillus subtilis is mixed in proportion, after fully mixing by same volume with the aqueous solution containing 1% pectin, 1.5% Tween 80,1.2% glycerine, 1% sweet mellow wine, 3% trehalose and 2% sodium acid carbonate, viable bacteria powder is made after spray-dried, again bacterium powder is mixed with selenide of carragheen, zinc methionine and glucose, show after detection, obtain probiotics every gram containing Lactobacillus plantarum 500,000,000, bacillus subtilis 2,500,000,000, the content of selenide of carragheen and zinc methionine is respectively 1.2% and 1.5%.By the probiotics of preparation in 1% ratio add in basal diet, feed to opening egg-layers after fully stirring.
After tissue and cell damage, except activating neutrophil leucocyte, macrophage and cause the product such as arachidonic acid and prostaglandin to discharge and the generation of oxygen radical, the inflammatory cytokines such as monocyte release TNF-a can also be stimulated.TNF-α is one of most important in cell factor, factor with initiating effect.IL-1 β be impel inflammation occur and by the inflammatory cytokine locally developing into whole body.Its secretion of IL-1 β is subject to TNF-α to regulate and control and work in coordination with TNF-α and together plays biological function.This research finds to stop raising the expression that probiotics causes inflammatory factor in each tissue, promotes that the inflammatory reaction of animal body occurs.
Detect stopping feeding inflammatory Cytokines Expression level in probiotics 15,45 days Laying-hen Serums respectively, result is as follows:
Table 3 stops feeding probiotics the impact on later stage batterylaying serum levels of inflammatory cytokines expression of laying eggs in 15,45 days
Note: 1. in table, data all represent with mean value ± standard error;
2. * represents that E and C compares, P<0.05 (n=10);
3. * * represents that E and C compares, P<0.01 (n=10).
As shown in table 3, compared with control group (C), when stopping feeding 15 days, (E) organizes TNF-α in Laying-hen Serum, IL-1 β, IL-2, IL-4 and INF-r decreases but not remarkable; Stop feeding after 45 days, (E) organizes TNF-α in Laying-hen Serum, IL-1 β, IL-2 and IL-4 concentration significantly reduces (P<0.05), and INF-r decreases but not remarkable; Infer that probiotics can suppress to organize the excessive generation of inflammatory cytokine, the damages of the factor pair tissue that reduces inflammation such as interior IL-1 β, TNF-α.
As Figure 1-3, for stopping feeding the probiotics impact on each tissue inflammation factor expression of later stage batterylaying of laying eggs in 15 days, compared with control group (C), when stopping feeding 15 days, (E) organize IL-1 β in Liver of Laying Hens, fallopian tubal, kidney and heart significantly to reduce (P<0.05), in liver and heart, TNF-α concentration significantly reduces (P<0.05); In liver, fallopian tubal and kidney, IL-2 concentration significantly reduces (P<0.05); Infer that probiotics can suppress to organize the excessive generation of inflammatory cytokine, the damages of the factor pair tissue that reduces inflammation such as interior IL-1 β, TNF-α.
As Figure 4-8, for stopping feeding the probiotics impact on each tissue inflammation factor expression of later stage batterylaying of laying eggs in 45 days, compared with control group (C), when stopping feeding 45 days, (E) organize IL-1 β and IL-2 in Liver of Laying Hens, kidney and heart significantly to reduce (P<0.05), in liver and heart, TNF-α concentration significantly reduces (P<0.05), IL-4 and INF-r decreases but not remarkable; Infer that probiotics can suppress to organize the excessive generation of inflammatory cytokine, the damages of the factor pair tissue that reduces inflammation such as interior IL-1 β, TNF-α.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. a preparation method for laying hen feeding micro-ecological preparation, is characterized in that, comprises the following steps:
(1) fowl lactobacillu plantarurn and fowl source Bacillus subtilis strain are mixed respectively after fermentation tank Zengjing Granule, obtain mixed bacteria liquid; The number that every gram of described laying hen feeding micro-ecological preparation contains fowl lactobacillu plantarurn is 2.5-5 hundred million, and the number that every gram of described laying hen feeding micro-ecological preparation contains fowl source bacillus subtilis is 10-25 hundred million; Described fowl lactobacillu plantarurn deposit number CCTCC NO:M2012079; Described fowl source bacillus subtilis CCTCC NO:M 2012078;
(2) described mixed bacteria liquid is fully mixed by same volume with the aqueous solution containing pectin, Tween 80, glycerine, sweet mellow wine, trehalose and sodium acid carbonate, after spray-dried, make microencapsulation viable bacteria powder; In the described aqueous solution, the mass percent of pectin, Tween 80, glycerine, sweet mellow wine, trehalose, sodium acid carbonate is respectively 0.5-1%, 0.8-1.5%, 0.8-1.2%, 0.5-1%, 3-6%, 2-3.5%;
(3) by described microencapsulation viable bacteria powder and selenide of carragheen, zinc methionine and auxiliary material glucose Homogeneous phase mixing, obtained laying hen feeding micro-ecological preparation, in described laying hen feeding micro-ecological preparation, the mass percent of selenide of carragheen is 0.05-0.12%, and in described laying hen feeding micro-ecological preparation, the mass percent of zinc methionine is 0.15-0.3%.
2. the application of laying hen feeding micro-ecological preparation as claimed in claim 1, by described laying hen feeding micro-ecological preparation in 1% ratio add in laying hens essential ration, feed to opening egg-layers after abundant stirring.
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| CN103966124A (en) * | 2014-04-23 | 2014-08-06 | 漳州大北农农牧科技有限公司 | Compound microecological preparation and additive, as well as premix thereof |
| CN105062916B (en) * | 2015-07-22 | 2017-12-12 | 江南大学 | Application of one bacillus coagulans on lifting laying hen egg yield |
| CN105494261A (en) * | 2015-12-30 | 2016-04-20 | 天津市寿南山禽蛋营养研发有限公司 | Method and nutrient for producing DHA (Docosahexaenoic Acid) and zinc-rich eggs |
| CN107518202A (en) * | 2016-06-21 | 2017-12-29 | 孙洪军 | A kind of bird feed additive |
| CN106954751B (en) * | 2017-02-17 | 2021-02-09 | 孙朝阳 | Method for feeding laying hens by using microecologics |
| CN107279586A (en) * | 2017-08-23 | 2017-10-24 | 合肥市明航养殖有限公司 | A kind of feed addictive for improving egg duck resistance against diseases |
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| CN101190003B (en) * | 2006-12-01 | 2010-12-01 | 北京东方联鸣科技发展有限公司 | High-efficiency biological active fodder additives products and producing method and application thereof |
| CN101278702B (en) * | 2007-04-05 | 2011-03-23 | 北京大北农科技集团股份有限公司 | Novel microbial feed additive and method of producing the same |
| CN102067941B (en) * | 2010-12-09 | 2012-11-28 | 四川省食品发酵工业研究设计院 | Method for producing compound microbial preparation serving as fodder |
| CN102120975B (en) * | 2010-12-15 | 2012-06-20 | 山东宝来利来生物工程股份有限公司 | Bacillus subtilis strain with stronger bacteriostatic action and application thereof |
| CN102228235A (en) * | 2011-05-10 | 2011-11-02 | 江南大学 | Method for preparing probiotics microcapsule and its application |
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