CN104012803A - Preparation method of fermented feed capable of preventing cow mastitis - Google Patents
Preparation method of fermented feed capable of preventing cow mastitis Download PDFInfo
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Abstract
The invention provides a preparation method of a fermented feed capable of preventing cow mastitis. The preparation method comprises the following steps: weighing crushed crop straws; subjecting the crushed crop straws to water washing, acid washing, alkaline washing and secondary water washing in sequence; filtering the processed crop straws; concentrating the filter; inoculating the filter with strains such as several kinds of lactobacillus and bacillus subtilis, and allowing the mixture to perform aerobic fermentation and anaerobic fermentation, so as to produce fulvic acid; adding the obtained fulvic acid into a mixture material which is prepared by mixing corn flour, rice bran, distilled grain, bean pulp and other trace elements according to a certain proportion, and then adding 40% of water; inoculating the mixture solution with candidautilis to perform anaerobic fermentation, and finally obtaining the fermented feed which can directly feed cows. The formulation of the fermented feed is reasonable and nutritionally balanced, the somatic cell count in milk can be effectively reduced, the incidence of hidden mastitis of the cows is reduced, a good development mechanism of the mammary gland of cows is kept, and the milk yield and the milk quality of the cows are also improved.
Description
Technical field
The invention belongs to animal feed production technical field, especially relate to a kind of preparation method that can prevent the fermented feed of mastitis for milk cows.
Background technology
Mammitis is one of common disease of milk cow, and the incidence of disease is aborning up to 40%, and making output of milk decline 15%-20% is the important diseases that threatens dairy to develop in a healthy way.The clinical property mammitis of acute research is correlation R=0.5-0.7 with body cell, so various countries measure body cell as the index of detection mammitis and as the seed selection foundation of mastitis for milk cows resistance.According to the report milk cow dailys output such as Shook and recessive mastitis occurring degree, be extremely significantly negative correlation.Traditional prevention and cure method is mainly by breeding for disease resistance, is in addition to use antibiotic etc prevention and treatment mammitis, and the loss that reduces milk cattle cultivating industry needs a kind of environmental preparation of drug residue free, and biochemical fulvic acid also just arises at the historic moment.The biochemical fulvic acid macromolecule polyalcohol that to be a kind of organic matter produce through microorganism fermentation includes the multiple nutriments such as bioactive disease-resistant factor and amino acid, vitamine that have, and its biologically active has anti-inflammation hemostasia convergence antiallergy and adjusts in the effect daily ration of stomach and intestine function enhanced machine body immunocompetence and be added with on a small quantity health care and production-increasing function.The present invention, by Early-lactation milk cow feeding having been increased to the fermented feed of biochemical fulvic acid, studies its impact on Contents in Cows cell number and milk performance.
Summary of the invention
The problem to be solved in the present invention is to provide a kind of preparation method that can prevent the fermented feed of mastitis for milk cows, and the fermented feed that the method is produced can reduce somatic cell score prevention mammitis well, greatly improves production performance.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
The preparation method that can prevent the fermented feed of mastitis for milk cows, comprises the following steps:
(1) prepare biochemical fulvic acid: take the agricultural crop straw after pulverizing, through washing-pickling-alkali cleaning-secondary, wash four steps, abundant agitation and filtration, after filtrate is concentrated, inoculation various lactobacillus, bacillus subtilis, under certain fermentation condition, through aerobic fermentation, two kinds of fermentation modes of anaerobic fermentation, obtain biochemical fulvic acid;
(2) mixed material: the part by weight according to 10%-15% corn flour, 5%-7% rice bran, 4%-8% vinasse, 10%-18% dregs of beans and 2%-5% trace mineral supplement is evenly mixed to get mixed material, after biochemical fulvic acid that (1) is obtained join in described mixed material, finally adding weight ratio is 40% water;
(3) prepare fermented feed: by adding the mixed material inoculation candida utili of biochemical fulvic acid, then under certain condition of culture, carrying out anaerobic fermentation, the fermented feed of the milking cow that obtains directly throwing something and feeding.
Preferably, described in step (1), fermentation condition is: 28 ℃-30 ℃ of fermentation temperatures, inoculum concentration 10%, puts tank time 18～20h.
Preferably, in step (1), the described various lactobacillus of inoculation and the number ratio of described bacillus subtilis are (1.2-1.5): 1.
Preferably, trace mineral supplement described in step (2) comprises Organic Selenium 0.4%-0.9%, organic zinc 0.3-0.8%, biological anti-oxidant 0.2-0.5%, mould bacteria adsorbent 0.6-0.9%, vitamin 0.4-1.5%.
Preferably, described in above-mentioned steps (3), condition of culture is: inoculum concentration 50%, and cultivation temperature 20-27 ℃, cultivates after 2-3 days, reduce the temperature to 8-15 ℃, humidity 65-80%, ventilation 6.0L/min, incubation time 1-2 days, improve subsequently temperature to 20-27 ℃, humidity 75-90%, ventilation 5.0L/min, total incubation time 4-7 days.
The present invention has good effect:
1. the present invention has added bacillus subtilis, and the feed that the present invention produces contains very strong protease, lipase, amylase isoreactivity, can produce antibiotic, has compared with Johnson & Johnson's thing and take oxygen ability by force in animal intestinal; These characteristics are to promoting zootrophic feed conversion rate, diseases prevention and promotion growth of digesting and assimilating, improving animal to play an important role;
2. the present invention has added biochemical fulvic acid, and the feed of preparation has anti-inflammation hemostasia convergence antiallergy and adjusts stomach and intestine function, strengthens the effect of body immunity; Reduce the intrusion of breast pathogenic bacteria, reduced somatic number in the incidence of recessive mastitis and milk cow;
3. the present invention produces large number of biological enzyme by fermentation technique etc., as protease, lipase, amylase etc., helps animal to the degraded of feed, digestion, improves efficiency of feed utilization.In feed, not digested protein and some nitrogen substances are generated the materials such as putrescine, indoles, phenols with potential toxicity by Escherichia coli and the de-shuttle of other bacteriums in enteron aisle;
4. improve the output of milk of milk cow, reduce the incidence of disease of cow subclinical mastitis, for clinic mastitis, there is good result for the treatment of, no drug residue, to ox body stress not, safe and reliable;
In sum, the invention provides a kind of preparation method that can prevent the fermented feed of mastitis for milk cows, its formula rationally, balanced in nutrition, can effectively reduce somatic number in milk, reduce the incidence of cow subclinical mastitis, safeguard the good developmental mechanism of mammary gland system, improve milk production of cow and milk-quality.
The specific embodiment
Biochemical fulvic acid is the environment that simulating nature circle humic acid forms, a kind of active material forming with organic matter fermentations such as agricultural crop straws.The aromatic compound of the various bioactivators of its main component and the polymer of nitride, contain the functional groups such as phenolic hydroxyl group, carboxyl, alcoholic extract hydroxyl group, hydroxyl quinonyl, sulfonic group, amino, quinonyl, in its crystalline solid, contain fulvic acid sterling 62%, ribonucleic acid 15.92%, the several amino acids such as tryptophan and a large amount of B family vitamin, vitamin C, inositol, polysaccharide etc., its activity is 10 times of natural humus acid.Biochemical fulvic acid has high cation exchange capacity (CEC), sequestering power, buffer capacity, adsorption capacity and catalytic capability.Poultry is had to the function that promotes growth and increase immunity.
Bacillus subtilis is that China Ministry of Agriculture allows one of two kinds of bacillus as feed addictive, is developed into more and more feeding micro-ecological preparation.Because its preparation is nontoxic, noresidue, free of contamination " green " additive, in animal husbandry, feed industry extensive use.Bacillus subtilis has very strong protease, lipase, amylase isoreactivity, can produce antibiotic, has compared with Johnson & Johnson's thing and take oxygen ability by force in animal intestinal.These characteristics are to promoting zootrophic feed conversion rate, diseases prevention and promotion growth of digesting and assimilating, improving animal to play an important role.
Bacillus subtilis is a kind of anaerobic growth microorganism, can produce enzyme and nutriment.Research shows that bacillus can secrete a large amount of ectoenzymes, as protease, lipase, amylase etc., helps animal to the degraded of feed, digestion, improves efficiency of feed utilization.In feed, not digested protein and some nitrogen substances are generated the materials such as putrescine, indoles, phenols with potential toxicity by Escherichia coli and the de-shuttle of other bacteriums in enteron aisle.Some bacillus subtilises can produce the enzyme of amino oxidation, SOD enzyme, decomposing hydrogen sulfide and other antibacterial materials as hydrogen peroxide, play bactericidal action, thereby reduce the generation of the interior harmful substance of animal body.Bacillus produces SOD enzyme, and SOD can remove active oxygen radical in organism, reduces its toxic action to cell, and organism is escaped injury.Bacillus can produce volatile fatty acid in growth and breeding process, and as acetic acid, propionic acid, butyric acid etc., some aliphatic acid can reduce the pH of animal intestinal, thereby for the growth of lactic acid bacteria creates conditions, and the growth of inhibition germ.Wherein propionic acid can also participate in three shuttles acid circulations, for the metabolism of animal provides energy.Meanwhile, can produce very high Cobastab and vitamin C, for animal provides vitamin.Within 1996, Ozols studies and finds that bacillus is the main producer of Cobastab l and pyridoxamine 106 strain enteron aisle bacterium; Ni Xue in 1998 is diligent when waiting research to show that bacillus generation organic acid provides energy nutrient for body, also promotes animal absorbing calcium, phosphorus, iron.
Enteron aisle ancestral home flora is the dominant bacteria of enteron aisle, most anaerobism, and pathogenic bacteria mostly are aerobic bacteria.The artificial bacillus subtilis of introducing is after enteron aisle field planting, consume a large amount of free oxygens, cause anaerobic environment, can reduce the field planting of aerobic bacteria to enteron aisle, and be conducive to the growths of the anaerobic bacterias such as Bacillus acidi lactici and Bifidobacterium, cause the beneficial bacterium in body to increase and pathogenic bacteria minimizing, keep intestinal microecology system balancing.The mucous membrane binding sites such as bacillus and harmful bacteria competition basic nutrition material and enteron aisle, thus suppressing harmful bacteria reduces intestines problem, improves body resistance against diseases.Its toxic action to cell, escapes injury organism less.Bacillus can produce volatile fatty acid in growth and breeding process, and as acetic acid, propionic acid, butyric acid etc., some aliphatic acid can reduce the pH of animal intestinal, thereby for the growth of lactic acid bacteria creates conditions, and the growth of inhibition germ.
Many types bacillus and metabolite thereof have been widely used in food, feed, not yet find at present toxic and side effect, are a kind of safe bacterial classifications.Many bacillus and metabolin thereof are consciously or unconsciously used by people very early in food, medicine and feed, some bacillus belong to the bacterial classification of " generally recognized as safe (GRAs) ", the plurality of enzymes that bacillus metabolism produces, as cellulase, amylase, protease extensive use in food and feed.Bacillus is as probio to animal nonhazardous, and the meat egg food of producing is also safe.
Bacillus is easy to separation, cultivates and preserves, generally fairly simple to nutritional requirement, and accretion rate is than very fast, for industrial less demanding.Gemma has very strong resistance to heat, ultraviolet ray, ionising radiation and some chemicals in addition, can stand poor environment, such as can be in the existence of pH sour environment, also can grow in up to 80 ℃ of even higher environment in temperature.
With it, the native form in plant exists selenium element in Organic Selenium, can be digested better and retain in animal body, thereby help improve animal in the production performance in each stage, and need not face the threat of toxicity and the adverse effect that environment is caused.Selenium also participates in the formation of the synthetic and tissue of protein, studies confirm that, after use Organic Selenium, the energetic supersession of animal changes, and milk production of cow is significantly increased.It had just excreted before playing a role unlike inorganic selenium or artificial synthetic selenium; but can store in animal body and discharge when animal needs; because yeast selenium is present in polypeptide form in the protein of yeast cells, be therefore protected, and can in cud, do not explained.
Mammary gland surface coverage keratin, and after each lactation, latex dust also can secretory protein mucus seal latex dust.Therefore keratin is the first defence that lactating cow prevents intramammary infection.In this process, approximately there is 50% keratin to discharge and lose with milk at every turn.When keratin loses, need to be supplemented rapidly, otherwise can cause that latex dust has a superficial wound, cause that bacterium infects and makes the increase of Contents in Cows cell number then cause the raising of the mastitis for milk cows incidence of disease and affect quality and the output of milk of milk simultaneously.In addition, multiparity ox, because of protein synthesis rate reduction and the malabsorption to mineral matter, makes the synthetic deficiency of mammary gland and latex dust Epidermal Keratin cannot provide epithelial cell enough protections, and Contents in Cows cell is increased.Zinc is in conjunction with forming organic zinc after different amino acid and little peptide, can the overdraw absorption approach of mucous membrane of small intestine amino acid and little peptide of its absorption enters in tissue, its biological chelate structure is stable, is not subject to the impact of external environment and changes its solubility and not disturbed by other mineral.
The content of candida utili protein and Cobastab is all high than brewer's yeast, and it can using urea and nitric acid as nitrogenous source, does not need to add any growth factor to grow in culture medium.It can utilize pentose and hexose, can utilize the sulfite waste liquor of paper industry, can also utilize molasses, wood hydrolysis liquid etc. to produce the edible protein of people and animals.
Selection and the grouping of test ox
The healthy He Sitanniu that selects 240 lactation mid-terms, is divided into five groups according to close principles such as the output of milk, somatic cell score, parity and lactation number of days, and every 40 oxen are as one group.Before test, carry out t check, after indices difference is remarkable, do not test.
Embodiment 1
The preparation method that can prevent the fermented feed of mastitis for milk cows, comprises the following steps:
(1) prepare biochemical fulvic acid: take the agricultural crop straw after pulverizing, prepare biochemical fulvic acid, comprise the following steps:
1. washing: take the agricultural crop straw after pulverizing, with the running water rinsing impurity of 10 times, fully stir, filter, collect filter residue;
2. pickling: above-mentioned 1. middle filter residue is added to 5 times of water gagings, and salt adding acid for adjusting pH to 1.2, to 2.0, heats while stirring, in heating process, stir, test pH changes at 2～3 o'clock, then adds inorganic acid and be transferred to pH to 1.2 to 2.0, add thermal agitation, pH is raised to again 2～3, then adds inorganic acid, repeatedly for several times to pH to 1.2 till 2.0 constant, filter, the filter residue obtaining, washs three times to pH to 5.5-6.0 with running water washing filter residue, collects filter residue;
3. alkali cleaning: by above-mentioned 2. middle filter residue, the 2%NaOH that adds 10 times to it, heats while stirring half an hour, standing half an hour, pH=9～10 of detection filtrate, filter, and collect filtrate;
4. secondary washing: the abundant agitation and filtration of filtrate to obtaining in 3., concentrates filtrate;
5. after concentrating, filtrate is inoculated various lactobacillus, bacillus subtilis, in 28 ℃ of fermentation temperatures, inoculum concentration 10%; Under fermentation condition, through aerobic fermentation, two kinds of fermentation modes of anaerobic fermentation, put tank time 18h and obtain biochemical fulvic acid; Wherein the number ratio of various lactobacillus and bacillus subtilis is 1.2:1.
(2) mixed material: the part by weight according to 10% corn flour, 5% rice bran, 5% vinasse, 10% dregs of beans and 3% trace mineral supplement is evenly mixed to get mixed material, after biochemical fulvic acid that (1) is obtained join in described mixed material, finally adding weight ratio is 40% water.
Described trace mineral supplement comprises Organic Selenium 0.4%, organic zinc 0.4%, biological anti-oxidant 0.3%, mould bacteria adsorbent 0.6%, vitamin 0.6%.
(3) prepare fermented feed: by adding the mixed material inoculation candida utili of biochemical fulvic acid, then carry out anaerobic fermentation, inoculum concentration 50%, cultivation temperature 20-27 ℃, cultivated after 2-3 days, reduced the temperature to 8-15 ℃, humidity 65-80%, ventilation 6.0L/min, incubation time 1-2 days, improves temperature to 25 ℃ subsequently, humidity 75-90%, ventilation 5.0L/min, total incubation time 4 days, the fermented feed of the milking cow that obtains directly throwing something and feeding.
The feed that embodiment 1 is produced carries out feeding, every day three times according to normal day flow vector to first group of milk.90 days experimental periods.
Embodiment 2
The preparation method that can prevent the fermented feed of mastitis for milk cows, comprises the following steps:
(1) prepare biochemical fulvic acid: take the agricultural crop straw after pulverizing, prepare biochemical fulvic acid, comprise the following steps:
1. washing: take the agricultural crop straw after pulverizing, with the running water rinsing impurity of 10 times, fully stir, filter, collect filter residue;
2. pickling: above-mentioned 1. middle filter residue is added to 5 times of water gagings, and salt adding acid for adjusting pH to 1.2, to 2.0, heats while stirring, in heating process, stir, test pH changes at 2～3 o'clock, then adds inorganic acid and be transferred to pH to 1.2 to 2.0, add thermal agitation, pH is raised to again 2～3, then adds inorganic acid, repeatedly for several times to pH to 1.2 till 2.0 constant, filter, the filter residue obtaining, washs three times to pH to 5.5-6.0 with running water washing filter residue, collects filter residue;
3. alkali cleaning: by above-mentioned 2. middle filter residue, the 2%NaOH that adds 10 times to it, heats while stirring half an hour, standing half an hour, pH=9～10 of detection filtrate, filter, and collect filtrate;
4. secondary washing: the abundant agitation and filtration of filtrate to obtaining in 3., concentrates filtrate;
5. after concentrating, filtrate is inoculated various lactobacillus, bacillus subtilis, in 30 ℃ of fermentation temperatures, inoculum concentration 10%; Under fermentation condition, through aerobic fermentation, two kinds of fermentation modes of anaerobic fermentation, put tank time 20h and obtain biochemical fulvic acid; Wherein the number ratio of various lactobacillus and bacillus subtilis is 1.4:1.
(2) mixed material: the part by weight according to 12% corn flour, 6% rice bran, 6% vinasse, 14% dregs of beans and 4% trace mineral supplement is evenly mixed to get mixed material, after biochemical fulvic acid that (1) is obtained join in described mixed material, finally adding weight ratio is 40% water.
Described trace mineral supplement comprises Organic Selenium 0.6%, organic zinc 0.7%, biological anti-oxidant 0.4%, mould bacteria adsorbent 0.7%, vitamin 1.1%.
(3) prepare fermented feed: by adding the mixed material inoculation candida utili of biochemical fulvic acid, then carry out anaerobic fermentation, inoculum concentration 50%, cultivation temperature 20-27 ℃, cultivated after 2-3 days, reduced the temperature to 8-15 ℃, humidity 65-80%, ventilation 6.0L/min, incubation time 1-2 days, improves temperature subsequently to 20-27 ℃, humidity 75-90%, ventilation 5.0L/min, total incubation time 7 days, the fermented feed of the milking cow that obtains directly throwing something and feeding.
The feed that embodiment 2 is produced carries out feeding, every day three times according to normal day flow vector to second group of milk.90 days experimental periods.
Embodiment 3
The preparation method that can prevent the fermented feed of mastitis for milk cows, comprises the following steps:
(1) prepare biochemical fulvic acid: take the agricultural crop straw after pulverizing, prepare biochemical fulvic acid, comprise the following steps:
1. washing: take the agricultural crop straw after pulverizing, with the running water rinsing impurity of 10 times, fully stir, filter, collect filter residue;
2. pickling: above-mentioned 1. middle filter residue is added to 5 times of water gagings, and salt adding acid for adjusting pH to 1.2, to 2.0, heats while stirring, in heating process, stir, test pH changes at 2～3 o'clock, then adds inorganic acid and be transferred to pH to 1.2 to 2.0, add thermal agitation, pH is raised to again 2～3, then adds inorganic acid, repeatedly for several times to pH to 1.2 till 2.0 constant, filter, the filter residue obtaining, washs three times to pH to 5.5-6.0 with running water washing filter residue, collects filter residue;
3. alkali cleaning: by above-mentioned 2. middle filter residue, the 2%NaOH that adds 10 times to it, heats while stirring half an hour, standing half an hour, pH=9～10 of detection filtrate, filter, and collect filtrate;
4. secondary washing: the abundant agitation and filtration of filtrate to obtaining in 3., concentrates filtrate;
5. after concentrating, filtrate is inoculated various lactobacillus, bacillus subtilis, in 28 ℃-30 ℃ of fermentation temperatures, inoculum concentration 10%; Under fermentation condition, through aerobic fermentation, two kinds of fermentation modes of anaerobic fermentation, put tank time 18～20h and obtain biochemical fulvic acid; Wherein the number ratio of various lactobacillus and bacillus subtilis is 1.5:1.
(2) mixed material: the part by weight according to 15% corn flour, 7% rice bran, 7% vinasse, 17% dregs of beans and 4% trace mineral supplement is evenly mixed to get mixed material, after biochemical fulvic acid that (1) is obtained join in described mixed material, finally adding weight ratio is 40% water.
Described trace mineral supplement comprises Organic Selenium 0.9%, organic zinc 0.8%, biological anti-oxidant 0.4%, mould bacteria adsorbent 0.8%, vitamin 1.4%.
(3) prepare fermented feed: by adding the mixed material inoculation candida utili of biochemical fulvic acid, then carry out anaerobic fermentation, inoculum concentration 50%, cultivation temperature 20-27 ℃, cultivated after 2-3 days, reduced the temperature to 8-15 ℃, humidity 65-80%, ventilation 6.0L/min, incubation time 1-2 days, improves temperature subsequently to 20-27 ℃, humidity 75-90%, ventilation 5.0L/min, total incubation time 5 days, the fermented feed of the milking cow that obtains directly throwing something and feeding.
The feed that embodiment 3 is produced carries out feeding, every day three times according to normal day flow vector to the 3rd group of milk.90 days experimental periods.
Embodiment 4
The preparation method that can prevent the fermented feed of mastitis for milk cows, comprises the following steps:
Prepare additive, every 100g additive comprises: biological anti-oxidant 7g, mould bacteria adsorbent 11g, health breast precious 11g, biochemical fulvic acid 6g, Organic Selenium 4g, zinc 4g and vitamin E 0.4g; 100g/ (top margin of a page) is installed to be joined additive in the daily daily ration of the 4th group of milk cow.90 days experimental periods.
Embodiment 5
The test feed of the fermentation milk cow forage of lactic acid bacteria, bacillus subtilis as embodiment 5 will be contained on market.
The feed that embodiment 5 is produced carries out feeding, every day three times according to normal day flow vector to the 5th group of milk.90 days experimental periods.
Embodiment 6
Test feed using non-fermented milk ox feed common on market as embodiment 6.According to normal day flow vector, carry out feeding, every day three times to the 6th group of milk in the feed of embodiment 6.90 days experimental periods.
Measure detection method
(1) output of milk is measured
Adopt milk Room formula to concentrate and milk, jigger counting; Milk for one day three times, accumulative total morning, noon and afternoon sum is one day output of milk.Two days stature output of milk mean value of METHOD FOR CONTINUOUS DETERMINATION is counted once individual milk production of cow.
(2) conventional ingredients in milk analysis
Every 15 days, adopt milk sample once, according to the ratio of 4:3:3, get early, middle and late three mixed milks of milking.On-the-spot adding preservative agent 1-2 drips, 4 ℃ of preservations.Adopt milk composition automatic analyzer to measure the conventional ingredients in milks such as butterfat percnetage, milky white rate, lactose, milk dry matter.
(3) body cell detects
Adopt somatic cell counting instrument (Somacount 150), in the newborn routine of survey, above-mentioned newborn sample is carried out to Somatic Cell Count simultaneously.
Test results and analysis
(1) data of cows before test
Before on-test, first the basic data of test cows is investigated, record the age in days of giving milk, parity, daily milk yield and the milk-quality thereof of every ox as indexs such as butterfat percnetage, milky white rate, lactose, milk dry matters, particularly measured body cell quantity in milk.As shown in table 1, test result shows These parameters there was no significant difference between each group.
Table 1: cows statistical reliability data table before test
(2) impact of the feed that the present invention produces on milk production of cow
Duration of test, after one, two, three group of test Cow-feeding food all feeds, result shows that each test group daily yielding compares all and be improved with six groups, and battery of tests group is compared with six groups of control groups, and duration of test on average improves 3.02kg/d head; Two groups of test group with contrast six groups and compare, duration of test on average improves 2.27kg/d head; Three groups of test group with contrast six groups and compare, duration of test on average improves 2.30kg/d head; Four groups of test group are compared with six groups of control groups, and duration of test on average improves 1.28kg/d head; Five groups of test group are compared with six groups of control groups, and duration of test on average improves 1.70kg/d head;
1. one, two, three groups of test group are remarkable to milk production of cow raising; Test improves milk production of cow once, two, three groups of outputs of milk greatly from on-test, and between 90d, milk cow average daily milk production is ascendant trend a little, and stable performance, keeps the trend of the high output of milk always.
2. the 4th group just starts the output of milk effect of milk cow significantly, but on a declining curve after 45 days, illustrates that this feed is to improving the unstable properties of the output of milk;
3. the 5th group of 15 days output of milk rises, held stationary trend then, and stable performance, illustrates that the various enzymes that the fermented feed of lactic acid bacteria, bacillus subtilis produces play certain impact to the output of milk.On average, higher than control group, the per day output of milk has increase.Different tests group per day output of milk ascendant trend of whole experimental period refers to table 2.
Table 2: the different tests time is respectively organized output of milk statistical form (kg/d head)
(3) impact of feed on somatic number in milk in different embodiment
Milk cow feeding after five kinds of different prevention recessive mastitis feeds, all have different-effect.Test body cell average ratio in one group of milk and test the front lower 20.80 ten thousand/ml that fallen; Test body cell average ratio in two groups of milk and test the front lower 22.62 ten thousand/ml that fallen; Test body cell average ratio in three groups of milk and test the front lower 22.60 ten thousand/ml that fallen, test body cell average ratio in four groups and test the front lower 14.8 ten thousand/ml that fallen, test in five groups body cell average and risen 15.08 ten thousand/ml than test is front upper, contrast six groups in body cell average than test is front upper, risen 22.73 ten thousand/ml.
From whole experimental period variation tendency, test one, two, three group and greatly reduce body cell in milk cow, especially to feed after 45 days, milk somatic cell number is reduced to every milliliter of 400,000 left and right, and body cell number held stationary afterwards; From trend, test four groups in body cell average than test before greatly reduce Contents in Cows cell number; Test five groups and compare test front and back all less than declining, rise on the contrary; But test five groups, compare with six groups of control groups, whole or on a declining curve.
Test body cell downward trend in one, two, three group of milk and compare significant difference with other test group, the indirect corresponding relation occurring according to body cell quantity in milk and recessive mastitis, somatic number reduces and reduces with regard to respective description cow subclinical mastitis illness rate.Test somatic downward trend explanation biochemical fulvic acid in four groups and play very large impact in the process of prevention mastitis for milk cows.In each test group milk, the statistical analysis of body cell quantity is in Table 3.
Table 3: the different tests date is respectively organized somatic number statistical form (10 in milk
4individual/milliliter)
(4) impact of feed on butterfat, lactoprotein and Ruzhong dry in different embodiment
Table 4: test 60 days front and back and respectively organize milk conventional index statistical form
As can be seen from Table 4, give milk cow feeding experiment one, two, the feed of three groups, in reducing milk body cell quantity and increase the output of milk in, do not reduce butterfat percnetage in milk, milky white rate, the content of lactose and milk dry matter, and test one, two, the indices of three groups also has raising in various degree, test butterfat percnetage in five groups of milk, milky white rate, the content of lactose and milk dry matter has also has part to improve, test butterfat percnetage in four groups of milk, milky white rate, the content of lactose and milk dry matter does not change, illustrate that each index do not tested the impact of four groups of feeds.
Above-mentioned evidence, the fermented feed that can prevent mastitis for milk cows of preparing to the present invention of milk cow feeding, can reduce the incidence of cow subclinical mastitis, has improved milk production of cow.Wherein, feed prepared by the present invention that feeds, in milk, body cell average is than the 220000/ml that on average declined before testing, the average general 2.53kg of the duration of test output of milk, effect is significantly better than other group test products, and therefore, fermented feed prepared by the present invention can reduce the incidence of disease of cow recessive mastitis, for clinic mastitis, there is good result for the treatment of, no drug residue, to ox body stress not, safe and reliable, can improve the output of milk and the dairy food quality of milk cow, there is synergistic function simultaneously.
Above several embodiments of the present invention are had been described in detail, but described content is only preferred embodiment of the present invention, can not be considered to for limiting practical range of the present invention.All equalization variations of doing according to the present patent application scope and improvement etc., within all should still belonging to patent covering scope of the present invention.
Claims (5)
1. can prevent a preparation method for the fermented feed of mastitis for milk cows, it is characterized in that: comprise the following steps:
(1) prepare biochemical fulvic acid: take the agricultural crop straw after pulverizing, through washing-pickling-alkali cleaning-secondary, wash four steps, abundant agitation and filtration, after filtrate is concentrated, inoculation various lactobacillus, bacillus subtilis, under certain fermentation condition, through aerobic fermentation, two kinds of fermentation modes of anaerobic fermentation, obtain biochemical fulvic acid;
(2) mixed material: the part by weight according to 10%-15% corn flour, 5%-7% rice bran, 4%-8% vinasse, 10%-18% dregs of beans and 2%-5% trace mineral supplement is evenly mixed to get mixed material, after biochemical fulvic acid that (1) is obtained join in described mixed material, finally adding weight ratio is 40% water;
(3) prepare fermented feed: by adding the mixed material inoculation candida utili of biochemical fulvic acid, then under certain condition of culture, carrying out anaerobic fermentation, the fermented feed of the milking cow that obtains directly throwing something and feeding.
2. a kind of preparation method that can prevent the fermented feed of mastitis for milk cows according to claim 1, is characterized in that: described in step (1), fermentation condition is: 28 ℃-30 ℃ of fermentation temperatures, inoculum concentration 10%, puts tank time 18～20h.
3. a kind of preparation method that can prevent the fermented feed of mastitis for milk cows according to claim 1, is characterized in that: in step (1), the described various lactobacillus of inoculation and the number ratio of described bacillus subtilis are (1.2-1.5): 1.
4. a kind of preparation method that can prevent the fermented feed of mastitis for milk cows according to claim 1, it is characterized in that: trace mineral supplement described in step (2) comprises Organic Selenium 0.4%-0.9%, organic zinc 0.3-0.8%, biological anti-oxidant 0.2-0.5%, mould bacteria adsorbent 0.6-0.9%, vitamin 0.4-1.5%.
5. a kind of preparation method that can prevent the fermented feed of mastitis for milk cows according to claim 1, it is characterized in that: described in above-mentioned steps (3), condition of culture is: inoculum concentration 50%, cultivation temperature 20-27 ℃, cultivates after 2-3 days, reduce the temperature to 8-15 ℃, humidity 65-80%, ventilation 6.0L/min, incubation time 1-2 days, improve subsequently temperature to 20-27 ℃, humidity 75-90%, ventilation 5.0L/min, total incubation time 4-7 days.
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| CN104489246A (en) * | 2014-12-12 | 2015-04-08 | 韦文峰 | Selenium-enriched microbial feed additive for dairy cow and preparation method thereof |
| CN105166451A (en) * | 2015-08-06 | 2015-12-23 | 钱峰 | Cow feed capable of preventing and treating mammitis |
| CN108777975A (en) * | 2016-01-08 | 2018-11-09 | Can 科技公司 | animal feed pill and its manufacturing method |
| CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
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| CN110403071A (en) * | 2019-09-02 | 2019-11-05 | 田梅 | A kind of fermented feed of milk cow in lactation period |
| CN111418709A (en) * | 2020-05-07 | 2020-07-17 | 北京九州大地生物技术集团股份有限公司 | Feed for preventing dairy cow mastitis and preparation method thereof |
| CN115606681A (en) * | 2022-11-04 | 2023-01-17 | 安徽省农业科学院棉花研究所 | Broussonetia papyrifera leaf compound fermentation feed and preparation method thereof |
| CN116649464A (en) * | 2023-07-27 | 2023-08-29 | 山东昊日农牧生物技术有限公司 | Environment-friendly type fermented feed with tiepiece based on complex flora fermentation and preparation method thereof |
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