CN111418709A - Feed for preventing dairy cow mastitis and preparation method thereof - Google Patents

Feed for preventing dairy cow mastitis and preparation method thereof Download PDF

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Publication number
CN111418709A
CN111418709A CN202010395790.3A CN202010395790A CN111418709A CN 111418709 A CN111418709 A CN 111418709A CN 202010395790 A CN202010395790 A CN 202010395790A CN 111418709 A CN111418709 A CN 111418709A
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China
Prior art keywords
parts
feed
cfu
cow
mastitis
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CN202010395790.3A
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Chinese (zh)
Inventor
李婉
王岗
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Beijing Jiuzhou Dadi Biological Technology Group Co ltd
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Beijing Jiuzhou Dadi Biological Technology Group Co ltd
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Priority to CN202010395790.3A priority Critical patent/CN111418709A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/22Compounds of alkali metals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention provides a feed for preventing cow mastitis and a preparation method thereof, belongs to the technical field of cow breeding, and is prepared by fermenting the following raw materials in parts by weight: 50-70 parts of corn, 5-15 parts of bran, 20-30 parts of alfalfa, 5-15 parts of soybean meal, 0.2-0.4 part of baking soda, 0.5-1 part of salt, 3-8 parts of candida utilis, 1-3 parts of lactobacillus plantarum, 2-6 parts of lactobacillus acidophilus, 4-7 parts of bifidobacterium and 3-5 parts of corynebacterium glutamicum. The feed provided by the invention can reduce the somatic cells of the dairy cows, thereby preventing the occurrence of mastitis of the dairy cows. When the feed provided by the invention is fed, the number of somatic cells of a cow is reduced by 40-44 ten thousand per ml, and the number of somatic cells of a control group is increased by 17 ten thousand per ml, which shows that the feed provided by the invention can effectively prevent the occurrence and development of cow mastitis.

Description

Feed for preventing dairy cow mastitis and preparation method thereof
Technical Field
The invention belongs to the technical field of cow breeding, and particularly relates to a feed for preventing cow mastitis and a preparation method thereof.
Background
With the continuous improvement of the living standard of people, the milk becomes common food for dining tables of people. Milk is known to become a necessity in human life. The dairy cow breeding industry in China develops rapidly, and the breeding scale is continuously enlarged, however, mastitis of dairy cows is difficult to treat due to high morbidity, so that the dairy cow industry is greatly influenced, and the development of the dairy cow industry is severely restricted. Although the antibiotics have good effect on treating the cow mastitis, the antibiotics can remain in the cow milk, so that drug-resistant strains can be caused, the treatment effect on the cow is influenced, and the excrement can cause water and soil pollution.
Disclosure of Invention
In view of the above, the present invention aims to provide a feed for preventing mastitis in dairy cows and a preparation method thereof, wherein the feed provided by the present invention can reduce somatic cells of dairy cows, thereby preventing mastitis in dairy cows.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a feed for preventing cow mastitis, which is prepared by fermenting the following raw materials in parts by weight:
50-70 parts of corn, 5-15 parts of bran, 20-30 parts of alfalfa, 5-15 parts of soybean meal, 0.2-0.4 part of baking soda, 0.5-1 part of salt, 3-8 parts of candida utilis, 1-3 parts of lactobacillus plantarum, 2-6 parts of lactobacillus acidophilus, 4-7 parts of bifidobacterium and 3-5 parts of corynebacterium glutamicum.
Preferably, the number of viable bacteria of the Candida utilis is 1 × 1010cfu/g or more.
Preferably, the viable count of the lactobacillus plantarum is 2 × 1010cfu/g or more.
Preferably, the viable count of the lactobacillus acidophilus is 2 × 1011cfu/g or more.
Preferably, the viable count of the bifidobacteria is 3 × 1011cfu/g or more.
Preferably, the number of viable bacteria of the Corynebacterium glutamicum is 1 × 109cfu/g or more.
The invention also provides a preparation method of the feed, which comprises the following steps:
1) mixing the corn, the bran, the alfalfa, the soybean meal, the baking soda and the salt to obtain a mixture, and adjusting the water content of the mixture to be 30-40% to obtain a fermentation substrate;
2) mixing the fermentation substrate obtained in the step 1) with candida utilis, lactobacillus plantarum, lactobacillus acidophilus, bifidobacterium and corynebacterium glutamicum, and then fermenting to obtain the feed.
Preferably, the fermentation temperature is 25-35 ℃.
Preferably, the fermentation time is 3-5 days.
Preferably, the mixture has a water content of 35%.
The invention provides a feed for preventing cow mastitis, which is prepared by fermenting the following raw materials in parts by weight: 50-70 parts of corn, 5-15 parts of bran, 20-30 parts of alfalfa, 5-15 parts of soybean meal, 0.2-0.4 part of baking soda, 0.5-1 part of salt, 3-8 parts of candida utilis, 1-3 parts of lactobacillus plantarum, 2-6 parts of lactobacillus acidophilus, 4-7 parts of bifidobacterium and 3-5 parts of corynebacterium glutamicum. The feed provided by the invention can reduce the somatic cells of the dairy cows, thereby preventing the occurrence of mastitis of the dairy cows.
The results of the embodiments of the present invention show that: when the feed provided by the invention is fed, the number of somatic cells of a cow is reduced by 40-44 ten thousand per ml, and the number of somatic cells of a control group is increased by 17 ten thousand per ml, which shows that the feed provided by the invention can effectively prevent the occurrence and development of cow mastitis.
Detailed Description
The invention provides a feed for preventing cow mastitis, which is prepared by fermenting the following raw materials in parts by weight: 50-70 parts of corn, 5-15 parts of bran, 20-30 parts of alfalfa, 5-15 parts of soybean meal, 0.2-0.4 part of baking soda, 0.5-1 part of salt, 3-8 parts of candida utilis, 1-3 parts of lactobacillus plantarum, 2-6 parts of lactobacillus acidophilus, 4-7 parts of bifidobacterium and 3-5 parts of corynebacterium glutamicum.
The feed provided by the invention comprises 50-70 parts of corn by weight, preferably 55-65 parts of corn by weight, and more preferably 60 parts of corn by weight. The feed provided by the invention comprises 5-15 parts by weight of bran, preferably 8-12 parts by weight, and more preferably 10 parts by weight. The feed provided by the invention comprises 20-30 parts of alfalfa, preferably 22-28 parts of alfalfa, and more preferably 25 parts of alfalfa. The feed provided by the invention comprises 5-15 parts of soybean meal, preferably 8-12 parts of soybean meal, and more preferably 10 parts of soybean meal. The feed provided by the invention comprises 0.2-0.4 part by weight of baking soda, and preferably 0.3 part by weight of baking soda. The feed provided by the invention comprises 0.5-1 part by weight of salt, preferably 0.8 part by weight. In the present invention, the source of the above raw materials is not particularly limited, and a conventional commercially available product may be used.
The feed provided by the invention comprises 3-8 parts by weight of candida utilis, preferably 5 parts by weight of candida utilis, and the viable count of the candida utilis is 1 × 1010cfu/g or more. The Candida utilis is not particularly limited in the source thereof, and may be produced by a commercially available product or a conventional production method.
The feed provided by the invention comprises 1-3 parts by weight of lactobacillus plantarum, preferably 2 parts by weight of lactobacillus plantarum, and the viable count of the lactobacillus plantarum is 2 × 1010cfu/g or more. The source of the lactobacillus plantarum is not particularly limited, and the lactobacillus plantarum can be prepared by a commercially available product or a conventional preparation method.
The feed provided by the invention comprises 2-6 parts by weight of lactobacillus acidophilus, preferably 3-5 parts by weight of lactobacillus acidophilus, wherein the viable count of the lactobacillus acidophilus is 2 × 1011cfu/g or more. The source of the lactobacillus acidophilus is not particularly limited, and the lactobacillus acidophilus can be prepared by a commercially available product or a conventional preparation method.
The feed provided by the invention comprises 4-7 parts by weight of bifidobacteria, preferably 5-6 parts by weight of bifidobacteria, wherein the viable count of the bifidobacteria is 3 × 1011cfu/g or more. The source of the bifidobacterium is not particularly limited, and the bifidobacterium can be prepared by a commercial product or a conventional preparation method.
The feed provided by the invention comprises 3-5 parts by weight of corynebacterium glutamicum, preferably 4 parts by weight of corynebacterium glutamicum, wherein the viable count of the corynebacterium glutamicum is 1 × 109cfu/g or more. The source of the corynebacterium glutamicum is not particularly limited, and the corynebacterium glutamicum can be prepared by a commercially available product or a conventional preparation method.
The invention also provides a preparation method of the feed, which comprises the following steps:
1) mixing the corn, the bran, the alfalfa, the soybean meal, the baking soda and the salt to obtain a mixture, and adjusting the water content of the mixture to be 30-40% to obtain a fermentation substrate;
2) mixing the fermentation substrate obtained in the step 1) with candida utilis, lactobacillus plantarum, lactobacillus acidophilus, bifidobacterium and corynebacterium glutamicum, and then fermenting to obtain the feed.
The mixing mode of the corn, the bran, the alfalfa, the soybean meal, the baking soda and the salt is not particularly limited, and the corn, the bran, the alfalfa, the soybean meal, the baking soda and the salt are uniformly mixed by adopting a conventional mixing mode. In the invention, the water content of the mixture is 30-40%, and preferably 35%.
In the invention, the fermentation temperature is preferably 25-35 ℃, and more preferably 30 ℃; the fermentation time is preferably 3-5 d, and more preferably 4 d.
The feed for preventing the mastitis of the dairy cows is not specially limited in use method, and is used by being matched with coarse feed for feeding (the proportion of the coarse feed of the dairy cows in the lactation period is 40-60%). The feed intake of the feed for the dairy cows is not specially limited, and the feed can be taken freely.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A preparation method of a feed for preventing cow mastitis comprises the following steps:
1) mixing 50kg of corn, 5kg of bran, 20kg of alfalfa, 5kg of soybean meal, 0.2kg of baking soda and 0.5kg of salt to obtain a mixture, and adjusting the water content of the mixture to be 30% to obtain a fermented material;
2) mixing the obtained fermented material with 3kg Candida utilis, 1kg Lactobacillus plantarum, 2kg Lactobacillus acidophilus, 4kg Bifidobacterium and 3kg Corynebacterium glutamicum, and fermenting at 35 deg.C for 3d to obtain feed.
Wherein the number of viable Candida utilis is 1 × 1010cfu/g, the viable count of the lactobacillus plantarum is 2 × 1010cfu/g, viable count of Lactobacillus acidophilus 2 × 1011cfu/g, viable count of Bifidobacterium 3 × 1011cfu/g, viable count of Corynebacterium glutamicum 1 × 109cfu/g。
Example 2
A preparation method of a feed for preventing cow mastitis comprises the following steps:
1) mixing 70kg of corn, 15kg of bran, 30kg of alfalfa, 15kg of soybean meal, 0.4kg of baking soda and 1kg of salt to obtain a mixture, and adjusting the water content of the mixture to 40% to obtain a fermented material;
2) mixing the obtained fermented material with 8kg Candida utilis, 3kg Lactobacillus plantarum, 6kg Lactobacillus acidophilus, 7kg Bifidobacterium and 5kg Corynebacterium glutamicum, and fermenting at 25 deg.C for 5d to obtain feed.
Wherein the number of viable Candida utilis is 1 × 1010cfu/g, the viable count of the lactobacillus plantarum is 2 × 1010cfu/g, viable count of Lactobacillus acidophilus 2 × 1011cfu/g, viable count of Bifidobacterium 3 × 1011cfu/g, viable count of Corynebacterium glutamicum 1 × 109cfu/g。
Example 3
A preparation method of a feed for preventing cow mastitis comprises the following steps:
1) mixing 60kg of corn, 8kg of bran, 25kg of alfalfa, 10kg of soybean meal, 0.3kg of baking soda and 0.8kg of salt to obtain a mixture, and adjusting the water content of the mixture to 35% to obtain a fermented material;
2) mixing the obtained fermented material with 5kg Candida utilis, 2kg Lactobacillus plantarum, 3kg Lactobacillus acidophilus, 5kg Bifidobacterium and 4kg Corynebacterium glutamicum, and fermenting at 28 deg.C for 4d to obtain feed.
Wherein the number of viable Candida utilis is 1 × 1010cfu/g, the viable count of the lactobacillus plantarum is 2 × 1010cfu/g, viable count of Lactobacillus acidophilus 2 × 1011cfu/g, viable count of Bifidobacterium 3 × 1011cfu/g, viable count of Corynebacterium glutamicum 1 × 109cfu/g。
Example 4
The test groups are respectively the feeds prepared in the examples 1-3; the control group was a regular daily ration.
120 healthy lactating Holstein cows are selected and paired into 4 groups according to the principle that the number of fetuses, the weight, the number of lactating days and the daily milk yield are similar, and the test group and the control group are selected. The grouping is shown in table 1.
The daily ration structure of the test group and the control group is consistent with the feeding mode, and the feeding is carried out for 40 days. The cows are raised in bulk, fed 3 times, milked 3 times and drunk freely. The number of somatic cells in the milk was measured at the beginning and end of the experiment, respectively, and the results are shown in Table 1.
TABLE 1 number of somatic cells of cows
Figure BSA0000208540540000051
As can be seen from the table 1, when the feed provided by the invention is fed, the number of somatic cells of a cow is reduced by 40-44 ten thousand per ml, and the number of somatic cells of a control group is increased by 5 ten thousand per ml, which shows that the feed provided by the invention can effectively prevent the occurrence and development of cow mastitis.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. The feed for preventing the mastitis of the dairy cows is characterized by being obtained by fermenting the following raw materials in parts by weight:
50-70 parts of corn, 5-15 parts of bran, 20-30 parts of alfalfa, 5-15 parts of soybean meal, 0.2-0.4 part of baking soda, 0.5-1 part of salt, 3-8 parts of candida utilis, 1-3 parts of lactobacillus plantarum, 2-6 parts of lactobacillus acidophilus, 4-7 parts of bifidobacterium and 3-5 parts of corynebacterium glutamicum.
2. The feed of claim 1, wherein the Candida utilis has a viable count of 1 × 1010cfu/g or more.
3. The feed of claim 1, wherein the viable count of the Lactobacillus plantarum is 2 × 1010cfu/g or more.
4. The feed of claim 1, wherein the viable count of said lactobacillus acidophilus is 2 × 1011cfu/g or more.
5. The feed of claim 1, wherein the viable count of the bifidobacteria is 3 × 1011cfu/g or more.
6. The feed as claimed in claim 1, wherein the number of viable bacteria of Corynebacterium glutamicum is 1 × 109cfu/g or more.
7. A process for preparing a feed as claimed in any one of claims 1 to 6, comprising the steps of:
1) mixing the corn, the bran, the alfalfa, the soybean meal, the baking soda and the salt to obtain a mixture, and adjusting the water content of the mixture to be 30-40% to obtain a fermentation substrate;
2) mixing the fermentation substrate obtained in the step 1) with candida utilis, lactobacillus plantarum, lactobacillus acidophilus, bifidobacterium and corynebacterium glutamicum, and then fermenting to obtain the feed.
8. The method according to claim 7, wherein the fermentation temperature is 25 to 35 ℃.
9. The method according to claim 7 or 8, wherein the fermentation time is 3-5 days.
10. The method of claim 7, wherein the mixture has a water content of 35%.
CN202010395790.3A 2020-05-07 2020-05-07 Feed for preventing dairy cow mastitis and preparation method thereof Pending CN111418709A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116616366A (en) * 2023-05-31 2023-08-22 广州大台农饲料有限公司 Fermented nutritional composition for improving lactation capacity of sows as well as preparation method and application thereof

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Publication number Priority date Publication date Assignee Title
KR20060109582A (en) * 2005-04-16 2006-10-23 한국스테비아(주) Agent for reducing somatic cell of bulk milk
CN101253934A (en) * 2008-04-07 2008-09-03 中国农业科学院北京畜牧兽医研究所 Composite microorganism additive agent for milk cattle feed stuff and method of preparing the same
CN101700105A (en) * 2009-11-24 2010-05-05 黄建壮 Microecological feed additive for milk cows and preparation method thereof
CN103981123A (en) * 2014-03-28 2014-08-13 内蒙古和美科盛生物技术有限公司 Probiotic agent containing Lactobacillus plantarum and Bifidobacterium lactis, and its preparing method
CN104012803A (en) * 2014-06-12 2014-09-03 天津和正美科技发展有限公司 Preparation method of fermented feed capable of preventing cow mastitis

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060109582A (en) * 2005-04-16 2006-10-23 한국스테비아(주) Agent for reducing somatic cell of bulk milk
CN101253934A (en) * 2008-04-07 2008-09-03 中国农业科学院北京畜牧兽医研究所 Composite microorganism additive agent for milk cattle feed stuff and method of preparing the same
CN101700105A (en) * 2009-11-24 2010-05-05 黄建壮 Microecological feed additive for milk cows and preparation method thereof
CN103981123A (en) * 2014-03-28 2014-08-13 内蒙古和美科盛生物技术有限公司 Probiotic agent containing Lactobacillus plantarum and Bifidobacterium lactis, and its preparing method
CN104012803A (en) * 2014-06-12 2014-09-03 天津和正美科技发展有限公司 Preparation method of fermented feed capable of preventing cow mastitis

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116616366A (en) * 2023-05-31 2023-08-22 广州大台农饲料有限公司 Fermented nutritional composition for improving lactation capacity of sows as well as preparation method and application thereof
CN116616366B (en) * 2023-05-31 2023-11-07 广州大台农饲料有限公司 Fermented nutritional composition for improving lactation capacity of sows as well as preparation method and application thereof

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