CN106234855A - A kind of composite immune reinforcing agent of carp kidney cell - Google Patents
A kind of composite immune reinforcing agent of carp kidney cell Download PDFInfo
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- CN106234855A CN106234855A CN201610923389.6A CN201610923389A CN106234855A CN 106234855 A CN106234855 A CN 106234855A CN 201610923389 A CN201610923389 A CN 201610923389A CN 106234855 A CN106234855 A CN 106234855A
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- reinforcing agent
- kidney cell
- immune reinforcing
- composite immune
- carp kidney
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Abstract
The invention belongs to technical field of aquaculture, be specifically related to the composite immune reinforcing agent of a kind of carp kidney cell, be made up of following raw material: Flos Caryophylli extract, curcumin, Fructus Schisandrae Chinensis lyophilized powder, thiamine hydrochloride, Lins eed protein, Freeze-dry Powder of Probioctics, pachyman.The present invention has the advantage that in the present invention, composite immune reinforcing agent is after carp kidney cell is fed compared to existing technology, its the intestines and stomach Microflora, kind and the balance of ratio can be regulated, suppress the growth of former bacterium, improve immunoglobulin concentrations, strengthen the growth of immune organ, stimulate juvenile fish to set up self immune system ahead of time；Plurality of raw materials mating reaction, the secretion of facilitating digestion enzyme, improves the raising of digestive and absorptive functions, and then improves its production performance, reduce the generation of digestive tract disease, improve juvenile fish survival rate；Preparation method is simple, it is easy to promote.
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to the composite immune reinforcing agent of a kind of carp kidney cell.
Background technology
Cyprinus carpio is that in freshwater fish, kind is at most, distribution is the widest, it is with the longest history to cultivate, one of yield soprano, adapts to
Property strong, the drug effect that its squama, brain, blood, mesh and Pi Junyou are certain, therefore it is more and more cultivated by people and eats, but due to
Juvenile fish stage physiological function and developing immune system are incomplete, cause carp kidney cell survival rate relatively low, may make adult fish simultaneously
Meat is poor, and weight is relatively low, it is therefore desirable to study the immunity how improving carp kidney cell.
Summary of the invention
It is an object of the invention to for existing problem, it is provided that the composite immune reinforcing agent of a kind of carp kidney cell.
The present invention is achieved by the following technical solutions: a kind of composite immune reinforcing agent for carp kidney cell, by with
The raw material composition of lower weight portion: Flos Caryophylli extract 4-8, curcumin 7-10, Fructus Schisandrae Chinensis lyophilized powder 8-15, thiamine hydrochloride 2-6, sub-
Fiber crops seed albumen 1-4, Freeze-dry Powder of Probioctics 3-6, pachyman 15-20；
Wherein, described Freeze-dry Powder of Probioctics is Lactobacillus Jensenii, bifidobacterium longum and the mixture of Raman lactobacillus, its weight ratio
For 2-5:2-3:1.
As further improvement of these options, described raw material mixes respectively after super micron mill is pulverized, after mixing
Cross 200 mesh sieves.
As further improvement of these options, the addition in feedstuff is the 0.5-1% of feed relative.
The present invention has the advantage that in the present invention, composite immune reinforcing agent is fed for carp kidney cell compared to existing technology
After, it is possible to regulate its intestines and stomach Microflora, kind and the balance of ratio, suppress the growth of former bacterium, improve immunoglobulin concentrations,
Strengthen the growth of immune organ, stimulate juvenile fish to set up self immune system ahead of time；Plurality of raw materials mating reaction, facilitating digestion enzyme
Secretion, improves the raising of digestive and absorptive functions, and then improves its production performance, reduces the generation of digestive tract disease, improves juvenile fish
Survival rate；Preparation method is simple, it is easy to promote.
Detailed description of the invention
Embodiment 1
A kind of composite immune reinforcing agent for carp kidney cell, is made up of the raw material of following weight portion: Flos Caryophylli extract 4, Rhizoma Curcumae Longae
Element 710, Fructus Schisandrae Chinensis lyophilized powder 8, thiamine hydrochloride 6, Lins eed protein 1, Freeze-dry Powder of Probioctics 6, pachyman 15；
Wherein, described Freeze-dry Powder of Probioctics is Lactobacillus Jensenii, bifidobacterium longum and the mixture of Raman lactobacillus, its weight ratio
For 4:3:1.
Preparation method for the composite immune reinforcing agent of carp kidney cell is, by raw material respectively after super micron mill is pulverized
Mixing, crosses 200 mesh sieves after mixing.
In actual use, the addition in feedstuff is the 0.8% of feed relative.
Embodiment 2
A kind of composite immune reinforcing agent for carp kidney cell, is made up of the raw material of following weight portion: Flos Caryophylli extract 6, Rhizoma Curcumae Longae
Element 8, Fructus Schisandrae Chinensis lyophilized powder 12, thiamine hydrochloride 4, Lins eed protein 2, Freeze-dry Powder of Probioctics 3, pachyman 16；
Wherein, described Freeze-dry Powder of Probioctics is Lactobacillus Jensenii, bifidobacterium longum and the mixture of Raman lactobacillus, its weight ratio
For 3:3:1.
Its preparation method is in the same manner as in Example 1 with using method.
Embodiment 3
A kind of composite immune reinforcing agent for carp kidney cell, is made up of the raw material of following weight portion: Flos Caryophylli extract 8, Rhizoma Curcumae Longae
Element 7, Fructus Schisandrae Chinensis lyophilized powder 8, thiamine hydrochloride 6, Lins eed protein 2, Freeze-dry Powder of Probioctics 6, pachyman 18；
Wherein, described Freeze-dry Powder of Probioctics is Lactobacillus Jensenii, bifidobacterium longum and the mixture of Raman lactobacillus, its weight ratio
For 5:2:1.
Its preparation method is in the same manner as in Example 1 with using method.
Embodiment 4
A kind of composite immune reinforcing agent for carp kidney cell, is made up of the raw material of following weight portion: Flos Caryophylli extract 6, Rhizoma Curcumae Longae
Element 10, Fructus Schisandrae Chinensis lyophilized powder 8, thiamine hydrochloride 3, Lins eed protein 3, Freeze-dry Powder of Probioctics 5, pachyman 16；
Wherein, described Freeze-dry Powder of Probioctics is Lactobacillus Jensenii, bifidobacterium longum and the mixture of Raman lactobacillus, its weight ratio
For 2:3:1.
Its preparation method is in the same manner as in Example 1 with using method.
Randomly choosing 1500 urosome types uniform, body colour is normal, and vitality is good, and 4-5 age in days, body weight is the Cyprinus carpio of 2 ± 0.3g
Fish juvenile fish, as experimental subject, is randomly divided into 5 groups, and experimental group 1 uses composite immune reinforcing agent according to method in embodiment 1；Real
Test in group 2 and pachyman in embodiment 1 raw material is replaced with the zymosan of corresponding weight；By embodiment 1 raw material in experimental group 3
Middle Fructus Schisandrae Chinensis lyophilized powder is removed, and other components are identical；Thiamine hydrochloride in embodiment 1 raw material is removed by experimental group 4, other groups
Split-phase is same；Experimental group 5 feeds according to a conventional method, without composite immune reinforcing agent；Other management methods are identical, and record is relevant
Data, the Gain weight obtaining juvenile fish is as follows:
Table 1
| Group | Amount of survival (tail) | Survival rate (%) | Average weight (g) | Feed coefficient |
| Experimental group 1 | 297 | 99.0 | 102.5 | 1.26 |
| Experimental group 2 | 275 | 91.7 | 96.4 | 1.43 |
| Experimental group 3 | 259 | 86.3 | 80.7 | 1.79 |
| Experimental group 4 | 256 | 85.3 | 83.6 | 1.65 |
| Experimental group 5 | 232 | 77.3 | 62.4 | 2.04 |
By data in table 1 it can be seen that use in the present invention after composite immune reinforcing agent, the growth performance of Cyprinus carpio and survival rate
All have clear improvement.
After throwing something and feeding by the above process 20 days, often organize random choose 100, detect its intestinal bacteria number change, detection
Result is as follows:
Table 2
| Group | Anterior intestine (1 × 105) | Middle intestinal (1 × 105) | Hindgut (1 × 105) | Averagely (1 × 105) |
| Experimental group 1 | 9.6±0.1 | 5.7 | 6.8 | 7.4 |
| Experimental group 2 | 9.1 | 5.6 | 6.1 | 6.93 |
| Experimental group 3 | 7.2 | 4.2 | 4.9 | 5.43 |
| Experimental group 4 | 6.9 | 3.8 | 4.2 | 4.97 |
| Experimental group 5 | 1.3 | 0.92 | 2.8 | 1.67 |
Can be seen that Microflora increases in composite immune reinforcing agent can make Cyprinus carpio intestinal in the present invention, has by data in table 2
Effect improves the digestive function of body, promotes fish bulk-growth further.
Claims (3)
1. the composite immune reinforcing agent for carp kidney cell, it is characterised in that be made up of the raw material of following weight portion: Flos Caryophylli
Extract 4-8, curcumin 7-10, Fructus Schisandrae Chinensis lyophilized powder 8-15, thiamine hydrochloride 2-6, Lins eed protein 1-4, probiotic bacteria lyophilizing
Powder 3-6, pachyman 15-20；
Wherein, described Freeze-dry Powder of Probioctics is Lactobacillus Jensenii, bifidobacterium longum and the mixture of Raman lactobacillus, its weight ratio
For 2-5:2-3:1.
A kind of composite immune reinforcing agent for carp kidney cell, it is characterised in that described raw material is respectively
Mix after super micron mill is pulverized, after mixing, cross 200 mesh sieves.
A kind of composite immune reinforcing agent for carp kidney cell, it is characterised in that adding in feedstuff
Dosage is the 0.5-1% of feed relative.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610923389.6A CN106234855A (en) | 2016-10-22 | 2016-10-22 | A kind of composite immune reinforcing agent of carp kidney cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610923389.6A CN106234855A (en) | 2016-10-22 | 2016-10-22 | A kind of composite immune reinforcing agent of carp kidney cell |
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| CN106234855A true CN106234855A (en) | 2016-12-21 |
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| CN201610923389.6A Pending CN106234855A (en) | 2016-10-22 | 2016-10-22 | A kind of composite immune reinforcing agent of carp kidney cell |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107410753A (en) * | 2017-03-28 | 2017-12-01 | 陆川县恒伟养殖专业合作社 | A kind of selenium-rich fish meal and preparation method thereof |
| CN107647087A (en) * | 2017-11-21 | 2018-02-02 | 五河县金塘水产养殖有限公司 | A kind of bait for strengthening carp kidney cell resistance against diseases |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076713A (en) * | 2015-09-30 | 2015-11-25 | 通威股份有限公司 | Composite feed capable for improving intestinal health of tilapia and preparation method of composite feed |
-
2016
- 2016-10-22 CN CN201610923389.6A patent/CN106234855A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076713A (en) * | 2015-09-30 | 2015-11-25 | 通威股份有限公司 | Composite feed capable for improving intestinal health of tilapia and preparation method of composite feed |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107410753A (en) * | 2017-03-28 | 2017-12-01 | 陆川县恒伟养殖专业合作社 | A kind of selenium-rich fish meal and preparation method thereof |
| CN107647087A (en) * | 2017-11-21 | 2018-02-02 | 五河县金塘水产养殖有限公司 | A kind of bait for strengthening carp kidney cell resistance against diseases |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161221 |