CN104839426A - Biologically-fermented feed and preparation method thereof - Google Patents

Biologically-fermented feed and preparation method thereof Download PDF

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CN104839426A
CN104839426A CN201510206609.9A CN201510206609A CN104839426A CN 104839426 A CN104839426 A CN 104839426A CN 201510206609 A CN201510206609 A CN 201510206609A CN 104839426 A CN104839426 A CN 104839426A
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feed
fermentation
root
biofermentation
honeysuckle
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CN104839426B (en
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宋鹏
杨赛
孙立枢
任付平
李爱江
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Tianjin Naer Biotechnology Co Ltd
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Henan University of Science and Technology
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Abstract

The invention specifically relates to a biologically-fermented feed and a preparation method thereof, belonging to the field of biological feeds. The biologically-fermented feed for pigs provided by the invention comprises traditional Chinese herbal components like rhubarb, baikal skullcap root, ladybell root, sandalwood, isatis root, honeysuckle, etc., so anti-stress ability and disease resistance of the pigs are improved, and the level of cholesterol in pig blood is reduced; meanwhile, the feed also comprises pig-available biologically-fermented protein, fat, mineral substances, vitamins and various high-energy substances, so the feed is rich in nutrients; the feed further comprises numerous active and non-active beneficial microorganisms capable of providing original prebiotic substances for gastrointestinal microorganisms, improving the survival environment of the gastrointestinal microorganisms and regulating growth and reproduction of the microorganisms to allow all the gastrointestinal microorganisms to be in a state of normal rapid development and be capable of promoting peristalsis and reducing gastrointestinal diseases; thus, the feed can effectively promote weight gain of the pigs, improve the quality of pork and make the pork tender and delicious while reducing feeding of concentrate and lowering cost.

Description

A kind of biological fermentation feed and preparation method thereof
Technical field
The invention belongs to biological feedstuff field, be specifically related to a kind of biological fermentation feed and preparation method thereof.
Background technology
Along with the raising of people's living standard, the demand for pollution-free food, pollution-free food is more and more higher, and pork and internal organs thereof receive much concern especially as the meat that Chinese people are traditional.At present, the common feedstuffs kind of pig is a lot, is divided into the large class of protein feeds, energy feed, roughage, green forage, ensilage, mineral feed and feed addictive 7 by nutrition.Although all kinds of feed has respective distinctive effect, also have requirement to the feed collocation of the pig of various different cultivars, the ANFs particularly in vegetalitas, animality and mineral material is unfavorable for digesting and assimilating of pig.Meanwhile, in the production of pork pig, cause excessive use antibiotic and abuse prohibited items because of eager for instant success and quick profits, the harmful substance in animal food is residual may exceed standard, and has a strong impact on the product quality of pork, thus affects the healthy of people.Therefore, for the different approaches of pork product, mode and degree, research environment-friendly feed substitute, replaces those feed being easy to cause pork to pollute or additives, thus produce the pork product of high-quality safety-type, become a new trend of future in feed industry.
Biological fermentation feed refers under manual control condition, microorganism is by the metabolic activity of self, decomposed by ANFs in vegetalitas, animality and mineral material or transform, generation more can be searched for food by livestock and poultry, digests, absorb and the feedstuff of nonhazardous effect.
At present, biological fermentation feed exists that bacterial classification source is complicated, trophic level is low, to defects such as the enteron aisle improvement result of pig are limited, these are not only relevant to fermented bacterium and zymotechnique, and fermentation substrate also has a certain impact to it.
Summary of the invention
The object of this invention is to provide a kind of biological fermentation feed and preparation method thereof.Effectively can improve immunity, promote appetite, improve food-intake, drain smoothly, anti-bacteria and anti-virus, reduce the generation of disease.
The present invention for the taked technical scheme that solves the problem is, a kind of biological fermentation feed, described feed is made up of biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle, and the parts by weight preparing each component needed for feed are respectively biofermentation protein 10-12 parts, rheum officinale 1-1.5 part, root of large-flowered skullcap 2-4 part, adenophora tetraphylla 1-2 part, santal 1-1.5 part, Radix Isatidis 1-2 part, honeysuckle 2-3 part;
Described biofermentation albumen with wheat bran, dregs of beans, sweet potato waste, pomace for fermentation substrate, with urea and sodium chloride for additive, with geotrichum candidum, bacillus megaterium and trichoderma reesei for fermented bacterium fermentation forms.
The preparation method of described biological fermentation feed, comprises the following steps:
Step one, pulverizing
Rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, after pulverizing with pulverizer, crosses 40 mesh sieves, for subsequent use;
Step 2, fermentation substrate pretreatment
By wheat bran, dregs of beans, sweet potato waste and pomace by weight mixing for 5:1:2:2 and adding urea and sodium chloride after stirring, obtained fermentate, and the weight ratio of wheat bran, dregs of beans, sweet potato waste, pomace, urea and sodium chloride is 100:20:40:40:4:1, again by fermentate and water by volume for 20:3 mixes and stir, namely obtained fermentation substrate, for subsequent use;
Step 3, inoculation bacterial classification
In fermentation substrate, first add trichoderma reesei stir, 30 DEG C of fermentations after 36 hours, then add bacillus megaterium and geotrichum candidum, and the inoculation total amount of bacterial classification is 3%, and each bacterial classification number is than being geotrichum candidum: bacillus megaterium: trichoderma reesei=3:2:1;
Step 4, fermentation
After step 3 inoculation being had the fermentation substrate 30 DEG C fermentation 36h of bacterial classification, take out tunning and put into the drying chamber inner drying that temperature is 80-90 DEG C, until the water content of tunning is lower than 10%, obtained biofermentation albumen, for subsequent use;
The preparation of step 5, biological fermentation feed
Take biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle by the parts by weight of described each component, after stirring, namely obtain biological fermentation feed.
Geotrichum candidum in the present invention is preserved in China General Microbiological culture presevation administrative center, and preservation bacterium number is CGMCC 2.1132.Bacillus megaterium is preserved in China General Microbiological culture presevation administrative center, and preservation bacterium number is CGMCC 1.2416.Trichoderma reesei is preserved in China General Microbiological culture presevation administrative center, and preservation bacterium number is CGMCC 3.5218.Above bacterial classification is purchased from Shandong abyssopelagic organism Science and Technology Co., Ltd..
Effect of each component in the present invention:
Sorrel rhubarb stem is red, gas delicate fragrance, bitter and micro-puckery, and that chews sticks to one's teeth, and has sand feeling.Have and attack stagnant, clearing away damp-heat, purging intense heat, cool blood, effect such as the stasis of blood, removing toxic substances of dispelling.The root of large-flowered skullcap is Labiatae Scutellaria herbaceos perennial, and root is used as medicine, bitter, cold in nature, has heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, the effect such as antiabortive.Cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swell the diseases such as furuncle sore.The clinical antibiotic property of the root of large-flowered skullcap is better than the coptis, and does not develop immunity to drugs.Adenophora tetraphylla is Campanulaceae herbaceos perennial, begin be loaded in Eastern Han Dynasty's Shennong's Herbal, be classified as top grade, and describe have " main hematocele shies gas, except fever and chills, bowl spares benefit lung qi." function.Santal is the dry duramen of Santalaceae plant santal trunk.Cure mainly for cold obstruction causing qi stagnation, chest diaphragm does not relax, chest impediment and cardialgia, epigastric pain, and vomiting food is few.Radix Isatidis is the dry root of cruciferae isatis, usually excavates in the fall, can be used as medicine after the process of preparing Chinese medicine.It is cold in nature, and taste is micro-sweet rear bitterness first, has clearing heat and detoxicating, preventing cold, relieve sore throat effect.Be mainly used in the diseases such as dark reddish purple dark, the scarlet fever for the treatment of febrile virulent maculae, tongue.Honeysuckle is Caprifoliaceae woodbine honeysuckle and congener dry flower or is with the flower just opened.Its property sweet cold air fragrance, clear heat with drugs of sweet flavour and cold nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil.Honeysuckle can dispelling wind-heat, also kind removing summer-heat blood poison, for various febrile disease, as the disease such as body heat, dermexanthesis, spot, hot carbuncle carbuncle, abscess of throat, and equal Be very effective.
The main component of Chinese herbal medicine has alkaloid, polysaccharide, protein, organic acid, glucosides, flavone compound and terpenoid etc., mostly have nutrition and medical value concurrently, its effect increases immunity of organisms, improve organismic internal environment, improve premunition, anti-bacteria and anti-virus, improves a poor appetite, strengthen anti-stress effect, improve livestock and poultry production performance and product quality.Interpolation Chinese herbal medicine can solve in animal husbandry development process and endanger the healthy antibiotic residue of people and problem of environmental pollution, shortens feeding time simultaneously, reduces feeding cost, enhances productivity, have important economic implications and social benefit.
beneficial effect
Not only containing medicinal herb componentses such as rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckles in live pig biological fermentation feed of the present invention, the ability of live pig resisting stress and the resistance to disease can be improved, reduce live pig Blood Cholesterol level.Simultaneously also containing live pig utilizable biofermentation albumen, fat, mineral matter, vitamin, multiple high energy capacity material, nutritious, and a large amount of activity and nonactive beneficial microbe, these materials can provide prebiotic former class material to gastrointestinal microbiological, improve the living environment of gastrointestinal microbiological, regulate microbial growth and breed, making whole gastrointestinal microbiological be in a kind of situation of normal fast development, promote to wriggle, reduce gastrointestinal disease.While reducing fine fodder of feeding, reducing costs, effectively can promote live pig weightening finish, improve meat quality, make pork fresh and tender good to eat.
Detailed description of the invention
A kind of biological fermentation feed, described feed is made up of biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle, and the parts by weight preparing each component needed for feed are respectively biofermentation protein 10-12 parts, rheum officinale 1-1.5 part, root of large-flowered skullcap 2-4 part, adenophora tetraphylla 1-2 part, santal 1-1.5 part, Radix Isatidis 1-2 part, honeysuckle 2-3 part;
Described biofermentation albumen with wheat bran, dregs of beans, sweet potato waste, pomace for fermentation substrate, with urea and sodium chloride for additive, with geotrichum candidum, bacillus megaterium and trichoderma reesei for fermented bacterium fermentation forms.
The preparation method of described biological fermentation feed, comprises the following steps:
Step one, pulverizing
Rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, after pulverizing with pulverizer, crosses 40 mesh sieves, for subsequent use;
Step 2, fermentation substrate pretreatment
By wheat bran, dregs of beans, sweet potato waste and pomace by weight mixing for 5:1:2:2 and adding urea and sodium chloride after stirring, obtained fermentate, and the weight ratio of wheat bran, dregs of beans, sweet potato waste, pomace, urea and sodium chloride is 100:20:40:40:4:1, again by fermentate and water by volume for 20:3 mixes and stir, namely obtained fermentation substrate, for subsequent use;
Step 3, inoculation bacterial classification
In fermentation substrate, first add trichoderma reesei stir, 30 DEG C of fermentations after 36 hours, then add bacillus megaterium and geotrichum candidum, and the inoculation total amount of bacterial classification is 3%, and each bacterial classification number is than being geotrichum candidum: bacillus megaterium: trichoderma reesei=3:2:1;
Step 4, fermentation
After step 3 inoculation being had the fermentation substrate 30 DEG C fermentation 36h of bacterial classification, take out tunning and put into the drying chamber inner drying that temperature is 80-90 DEG C, until the water content of tunning is lower than 10%, obtained biofermentation albumen, for subsequent use;
The preparation of step 5, biological fermentation feed
Take biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle by the parts by weight of described each component, after stirring, namely obtain biological fermentation feed.
Embodiment 1
A kind of biological fermentation feed, described feed is made up of biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal and honeysuckle, and the parts by weight preparing each component needed for feed are respectively biofermentation protein 10 part, rheum officinale 1 part, the root of large-flowered skullcap 2 parts, adenophora tetraphylla 1 part, 1 part, santal, Radix Isatidis 1 part, honeysuckle 2 parts;
Described biofermentation albumen with wheat bran, dregs of beans, sweet potato waste, pomace for fermentation substrate, with urea and sodium chloride for additive, with geotrichum candidum, bacillus megaterium and trichoderma reesei for fermented bacterium fermentation forms.
The preparation method of described biological fermentation feed, comprises the following steps:
Step one, pulverizing
Rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, after pulverizing with pulverizer, crosses 40 mesh sieves, for subsequent use;
Step 2, fermentation substrate pretreatment
By wheat bran, dregs of beans, sweet potato waste and pomace by weight mixing for 5:1:2:2 and adding urea and sodium chloride after stirring, obtained fermentate, and the weight ratio of wheat bran, dregs of beans, sweet potato waste, pomace, urea and sodium chloride is 100:20:40:40:4:1, again by fermentate and water by volume for 20:3 mixes and stir, namely obtained fermentation substrate, for subsequent use;
Step 3, inoculation bacterial classification
In fermentation substrate, first add trichoderma reesei stir, 30 DEG C of fermentations after 36 hours, then add bacillus megaterium and geotrichum candidum, and the inoculation total amount of bacterial classification is 3%, and each bacterial classification number is than being geotrichum candidum: bacillus megaterium: trichoderma reesei=3:2:1;
Step 4, fermentation
After step 3 inoculation being had the fermentation substrate 30 DEG C fermentation 36h of bacterial classification, take out tunning and put into the drying chamber inner drying that temperature is 80-90 DEG C, until the water content of tunning is lower than 10%, obtained biofermentation albumen, for subsequent use;
The preparation of step 5, biological fermentation feed
Take biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle by the parts by weight of described each component, after stirring, namely obtain biological fermentation feed.
Embodiment 2
A kind of biological fermentation feed, described feed is made up of biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal and honeysuckle, and the parts by weight preparing each component needed for feed are respectively biofermentation protein 12 part, rheum officinale 1.5 parts, the root of large-flowered skullcap 4 parts, adenophora tetraphylla 2 parts, 1.5 parts, santal, Radix Isatidis 2 parts, honeysuckle 3 parts;
Described biofermentation albumen with wheat bran, dregs of beans, sweet potato waste, pomace for fermentation substrate, with urea and sodium chloride for additive, with geotrichum candidum, bacillus megaterium and trichoderma reesei for fermented bacterium fermentation forms.
The preparation method of described biological fermentation feed, comprises the following steps:
Step one, pulverizing
Rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, after pulverizing with pulverizer, crosses 40 mesh sieves, for subsequent use;
Step 2, fermentation substrate pretreatment
By wheat bran, dregs of beans, sweet potato waste and pomace by weight mixing for 5:1:2:2 and adding urea and sodium chloride after stirring, obtained fermentate, and the weight ratio of wheat bran, dregs of beans, sweet potato waste, pomace, urea and sodium chloride is 100:20:40:40:4:1, again by fermentate and water by volume for 20:3 mixes and stir, namely obtained fermentation substrate, for subsequent use;
Step 3, inoculation bacterial classification
In fermentation substrate, first add trichoderma reesei stir, 30 DEG C of fermentations after 36 hours, then add bacillus megaterium and geotrichum candidum, and the inoculation total amount of bacterial classification is 3%, and each bacterial classification number is than being geotrichum candidum: bacillus megaterium: trichoderma reesei=3:2:1;
Step 4, fermentation
After step 3 inoculation being had the fermentation substrate 30 DEG C fermentation 36h of bacterial classification, take out tunning and put into the drying chamber inner drying that temperature is 80-90 DEG C, until the water content of tunning is lower than 10%, obtained biofermentation albumen, for subsequent use;
The preparation of step 5, biological fermentation feed
Take biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle by the parts by weight of described each component, after stirring, namely obtain biological fermentation feed.
Test the impact of 1 biological fermentation feed on grower pigs production performance
The pig 45 that parity, age in days and body weight are substantially close is selected in test, is divided into I, II and III tri-groups at random, often organizes 15, male and female half and half.I group contrasts, and does not add any biological fermentation feed, basic mixed fodder of only feeding, and II group only adds biofermentation albumen on I group basis, and III group adds biological fermentation feed on I group basis.Test pig adopts group feeding, and day feeds 2 times, and freely drink water, other management is carried out according to a conventional method.Test first 5 days for preliminary trial period, feed management method is identical.As shown in table 1, grower pigs is fed after biofermentation albumen and biological fermentation feed, and weightening finish all significantly improves than control group I, reduces feed-weight ratio simultaneously, improves feed efficiency, saves feed cost, increases economic efficiency.
The weightening finish of table 1 test pig, feed intake and feed conversion ratio
Test the impact of 2 biological fermentation feeds on quality of pig trunk and internal organ proterties
Table 2 biological fermentation feed is on the impact of quality of pig trunk and internal organ proterties
As shown in table 2, Slaughter tests showed shows, the Visceral weights such as index and conscience such as each group of hog slaughtering rate, fat thickness, lean meat percentage all improved, slaughter experiment has no internal organs and observes pathology, show that biological fermentation feed is used as feedstuff for live pigs, also have the short long function of health care to quality of pig trunk and animal tissue's organ.

Claims (2)

1. a biological fermentation feed, it is characterized in that: described feed is made up of biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle, and the parts by weight preparing each component needed for feed are respectively biofermentation protein 10-12 parts, rheum officinale 1-1.5 part, root of large-flowered skullcap 2-4 part, adenophora tetraphylla 1-2 part, santal 1-1.5 part, Radix Isatidis 1-2 part, honeysuckle 2-3 part;
Described biofermentation albumen with wheat bran, dregs of beans, sweet potato waste, pomace for fermentation substrate, with urea and sodium chloride for additive, with geotrichum candidum, bacillus megaterium and trichoderma reesei for fermented bacterium fermentation forms.
2. the preparation method of a kind of biological fermentation feed according to claim 1, is characterized in that, comprise the following steps:
Step one, pulverizing
Rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, after pulverizing with pulverizer, crosses 40 mesh sieves, for subsequent use;
Step 2, fermentation substrate pretreatment
By wheat bran, dregs of beans, sweet potato waste and pomace by weight mixing for 5:1:2:2 and adding urea and sodium chloride after stirring, obtained fermentate, and the weight ratio of wheat bran, dregs of beans, sweet potato waste, pomace, urea and sodium chloride is 100:20:40:40:4:1, again by fermentate and water by volume for 20:3 mixes and stir, namely obtained fermentation substrate, for subsequent use;
Step 3, inoculation bacterial classification
In fermentation substrate, first add trichoderma reesei stir, 30 DEG C of fermentations after 36 hours, then add bacillus megaterium and geotrichum candidum, and the inoculation total amount of bacterial classification is 3%, and each bacterial classification number is than being geotrichum candidum: bacillus megaterium: trichoderma reesei=3:2:1;
Step 4, fermentation
After step 3 inoculation being had the fermentation substrate 30 DEG C fermentation 36h of bacterial classification, take out tunning and put into the drying chamber inner drying that temperature is 80-90 DEG C, until the water content of tunning is lower than 10%, obtained biofermentation albumen, for subsequent use;
The preparation of step 5, biological fermentation feed
Take biofermentation albumen, rheum officinale, the root of large-flowered skullcap, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle by the weight ratio of component each described in claim 1, after stirring, namely obtain biological fermentation feed.
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CN109770081A (en) * 2019-01-08 2019-05-21 广西富丽乡生物科技有限公司 A kind of method of microbial flora fermentation pannage
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WO2017128448A1 (en) * 2016-01-28 2017-08-03 深圳市七彩地农业科技有限公司 Non-cereal feed and preparation thereof and feeding method using same
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CN107410687A (en) * 2017-05-18 2017-12-01 佛山市高明区生产力促进中心 A kind of feed for being favorably improved live pig degree Fresh & Tender in Texture
CN109770081A (en) * 2019-01-08 2019-05-21 广西富丽乡生物科技有限公司 A kind of method of microbial flora fermentation pannage
CN109938189A (en) * 2019-04-25 2019-06-28 湖南加丰生物科技有限公司 A kind of live pig rheum officinale no-antibiotics feed and preparation method thereof

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