CN105076713A - Composite feed capable for improving intestinal health of tilapia and preparation method of composite feed - Google Patents
Composite feed capable for improving intestinal health of tilapia and preparation method of composite feed Download PDFInfo
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Abstract
The invention discloses a composite feed capable for improving the intestinal health of tilapia. The composite feed is obtained by mixing Chinese herbal medicine extracts and basic ingredients, and drying the mixture, wherein the weight ratio of the basic ingredients to the Chinese herbal medicine extracts is (995 to 999): (1-5); each 1 to 5 L of the Chinese herbal medicine extracts is obtained by conducting water extraction on the following Chinese herbal medicine: 2 to 10 g of panax notoginseng, 3 to 15 g of shizandra berry, 5 to 20 g of astragalus membranaceus,5 to 15 g of liquorice, 2 to 10 g of radix notoginseng, 6 to 18 g of lithospermum, 5 to 15 g of silybum marianum, and 2 to 10 g of fructus psoraleae. The composite feed is used for tilapia culture, and can be used for improving the intestinal health of tilapia, and the non-specific immunity of the organism of tilapia. The invention further provides a preparation method of the composite feed.
Description
Technical field
The present invention relates to fish meal technical field, particularly a kind of mixed feed improving Tilapia mossambica intestinal health, this mixed feed can be protected Tilapia mossambica intestinal health, improve body non-specific immunity, and the preparation method of this mixed feed.
Background technology
Natural animal-plant product taken from by Chinese herbal medicine, maintain its original composition, the natural form of structure and biologically active, containing compositions such as alkaloid, polysaccharide, saponin(e, volatile oil and organic acids, not only there are the effects such as extra-nutrition, growth promoting effects, develop immunitypty, also there is noresidue, without the resistance to the action of a drug, the distinguishing feature such as pollution-free, side effect is little.In recent years, Chinese herbal and crude drugs preparations has been widely used in aquaculture of aquatic animal, at promotion aquatic animal to grow, improves aquatic products product quality, improves production performance and strengthen in immunity of organisms etc. and reach better effects.Along with the raising of people's living standard, pay attention to food-safety problem, the feed safety as animal food source more and more comes into one's own.Current Chinese scholars is ensureing that cultivated animals is healthy, the research of promotion growth of animal, raising animal product quality etc. has invested noresidue, without the resistance to the action of a drug, free of contamination Chinese herbal and crude drugs preparations, probiotics and enzyme preparation.
Tilapia mossambica (Oreochromismossambicus) belongs to Perciformes, Callichthyidae, Tilapia mossambica genus, that China's export is earned foreign exchange one of important cultured fishes, advantages such as having fast growth, output is high, feeding habits are assorted, be nutritious, is subject to liking of domestic and international raiser and consumer deeply.Along with aquaculture intensivization development, Tilapia mossambica cultivation scale constantly expands, and cultivation density and cultured output constantly increase; Adding up China's Tilapia mossambica output in 2013 according to China Fisheries yearbook and reached 1,550,000 tons, occupies first place in the world in position.But along with Tilapia mossambica cultivation popularization, breeding density increase, excessively throw and raise and feed inferior use, Tilapia mossambica cultivation water environment goes from bad to worse, Tilapia mossambica liver and gall and intestines problem increased and day by day serious, already become one of key factor that restriction Tilapia mossambica aquaculture develops in a healthy way.South China's outburst Tilapia mossambica streptococcosis since 2009,2012 South China outbursts maximum streptococcosis since the dawn of human civilization, Tilapia mossambica drops in production over a large area, and cultured output glides rapidly, and raiser suffers heavy losses.In order to reduce cultivation loss, raiser uses the sulfa antibiotics such as sulphadiazine to mix to raise and throw something and feed, to reducing the death rate that Tilapia mossambica disease causes.But these violated antibiotic medicines add, and use causes the congested inflammation of enteron aisle, intestinal mucosa comes off, profitable strain is suppressed; Also cause the residual detection of Tilapia mossambica muscle medicine to exceed standard, outlet obstructions, the sustainable development of Tilapia mossambica aquaculture is obstructed simultaneously.
Therefore, for the problems such as the medicament residue caused are abused in the harm and sulfa antibiotics that solve intestines problem in current Tilapia mossambica hypoimmunity, breeding process, need a kind of nutritious, environmental protection of exploitation badly, Tilapia mossambica healthy growth can be promoted, improve efficiency of feed utilization, Shortening culturing period, improve intestinal health, improve meat quality, reduce the Tilapia mossambica mixed feed of medicament residue, this has important theoretical and practical significance for Tilapia mossambica healthy aquaculture and sustainable development.
Summary of the invention
Main purpose of the present invention is to provide a kind of feeding for Tilapia mossambica cultivation, intestinal tissue structural health can be improved, improve Tilapia mossambica intestine immunity function, safeguard the mixed feed that gut flora is stable, meanwhile, the present invention also provides the preparation method of above-mentioned mixed feed.
Technical scheme provided by the invention is: a kind of mixed feed improving Tilapia mossambica intestinal health, and described mixed feed is obtained after combination drying by Chinese herbal medicine extract and basic components; Described basic components and the part by weight of Chinese herbal medicine extract are 995 ~ 999:1 ~ 5;
Every 1 ~ 5kg Chinese herbal medicine extract is obtained by following Chinese herbal medicine water extraction:
Pseudo-ginseng 2 ~ 10g, the fruit of Chinese magnoliavine 3 ~ 15g, the Radix Astragali 5 ~ 20g, Radix Glycyrrhizae 5 ~ 15g, pseudo-ginseng 2 ~ 10g, Asian puccoon 6 ~ 18g, milk thistle 5 ~ 15g, Psoralea corylifolia 2 ~ 10g.
In the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, every 1 ~ 5kg Chinese herbal medicine extract is obtained by following Chinese herbal medicine water extraction: pseudo-ginseng 4 ~ 8g, the fruit of Chinese magnoliavine 10 ~ 15g, the Radix Astragali 7 ~ 15g, Radix Glycyrrhizae 10 ~ 15g, pseudo-ginseng 6 ~ 8g, Asian puccoon 12 ~ 18g, milk thistle 8 ~ 12g, Psoralea corylifolia 5 ~ 10g.
In the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, the basic components of every 100 weight portions comprises following parts by weight of component:
Wheat 3.0 ~ 13.0 parts, Strong flour 6.0 ~ 16.0 parts, the dish dregs of rice 5.0 ~ 15.0 parts, cotton dregs 3.0 ~ 12.0 parts, dregs of beans 15.0 ~ 40.0 parts, fish meal 2.0 ~ 10.0 parts, soyabean lecithin oil 0.5 ~ 3.0 part, soybean oil 2.0 ~ 5.0 parts, import DDGS8.0 ~ 20.0 part, cuttlefish cream 1.0 ~ 4.0 parts, fine bran 0.5 ~ 3.0 part, calcium dihydrogen phosphate 1 ~ 3 part, Choline Chloride 0.05 ~ 0.1 part, mineral substance premix 1 ~ 3 part, vitamin premix 0.1 ~ 0.5 part, compound probiotic 0.03 ~ 0.07 part.
In the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, the basic components of every 100 weight portions comprises following parts by weight of component: wheat 6.0 ~ 12.0 parts, Strong flour 8.0 ~ 14.0 parts, the dish dregs of rice 6.0 ~ 8.0 parts, cotton dregs 5.0 ~ 10.0 parts, dregs of beans 31.0 ~ 38.0 parts, fish meal 5.0 ~ 8.0 parts, soyabean lecithin oil 1 ~ 2.0 part, soybean oil 2.5 ~ 3.5 parts, import DDGS10.0 ~ 15.0 part, cuttlefish cream 1.5 ~ 2.5 parts, fine bran 1.5 ~ 2.0 parts, calcium dihydrogen phosphate 1.5 ~ 2 parts, Choline Chloride 0.05 ~ 0.08 part, mineral substance premix 1.5 ~ 2 parts, vitamin premix 0.25 ~ 0.3 part, compound probiotic 0.04 ~ 0.06 part.
In the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, described basic components also comprises mould inhibitor 0.03 part, antioxidant 0.03 part.
In the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, described vitamin premix is the omnivorefish vitamin premix F21V of Guangdong Tongwei Feed Co., Ltd.; Described mineral substance premix is the omnivorefish microelement pre-mix material F29M of Guangdong Tongwei Feed Co., Ltd.; Described compound probiotic main component is bacillus subtilis, and its specification is 100 ~ 800 × 10
9cFU/g, lactobacterium acidophilus, its specification is 1 ~ 10.0 × 10
9cFU/g.
The present invention also provides the preparation method of the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, comprises the steps
Step 1: take Chinese herbal medicine according to components by weight percent and carry out water extraction, and take basic components carry out first time mixing;
Step 2: basic components carried out second time mixing and adopt interior spraying adding technology to add in basic components by Chinese herbal medicine extract in the process;
Step 3: mixed for step 2 material is carried out extrusion granulation, and dry, cool.
In the preparation method of the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, in described step 2, after Chinese herbal medicine extract adds basic components to, mix 120 ~ 180 seconds.
In the preparation method of the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, in described step 3, the hot blast of 80 ~ 90 DEG C is adopted to carry out drying.
In the preparation method of the mixed feed of above-mentioned improved Tilapia mossambica intestinal health, the method for described Chinese herbal medicine extract water extraction is as follows:
Component takes Chinese herbal medicine by weight, and soaks 20 ~ 40min, adds 2.0-4.0L water, boil 30min-60min in every 50g Chinese herbal medicine, pours out liquid cooling; Add water 1.0 ~ 3.0L again in Chinese herbal medicine, boils 20min-60min, pours out liquid cooling, then the 1.0 ~ 3.0L that adds water in medicine, boils 20min-60min, pours out liquid cooling, three the liquid mixing in front and back, Chinese herbal medicine extract 1.0 ~ 5.0kg processed.
Beneficial effect of the present invention is as follows:
1. the present invention uses Chinese herbal medicine compatibility, and it comes from the natural component of plant, environmental protection, nonhazardous effect.This Chinese herbal medicine compatibility source is wide, cheap, easily obtain, and Chinese herbal medicine finished product preparation method is simple, and it is convenient to add in feed preparation, noresidue, can not enrichment, do not pollute breeding water body.
2. in mixed feed of the present invention, raise the Tilapia mossambica of different phase, all can improve the utilization rate of feed fat, energy, improve the circulation of intestines liver, safeguard that Tilapia mossambica intestinal beneficial flora is stablized, improve enteron aisle microvillus height, intestines wall circular muscle thickness and enteron aisle toughness, strengthen the secretion capacities such as intestinal mucosa non-specific immunity, enteron aisle digestive ferment.
3. this medicinal herb components also has the function of food calling, growth promotion, enhancing enteron aisle non-specific immunity.Different phase Growth Op Tilapia performance can be promoted, improve efficiency of feed utilization, strengthen body non-specific immunity and premunition, the Tilapia mossambica fillet antibiotic residue that prevention streptococcosis, reduction raiser use forbidden drug to cause, this invention has higher Social benefit and economic benefit.
4. the Chinese herb feed additive of the present invention's use, can improve liver of hybrid tilapia, intestinal protease and lipase active, promotes that Tilapia mossambica utilizes feed digestion, improves feed protein, lipid conversion capability.
Accompanying drawing explanation
Fig. 1 is intestinal epithelial cell microscope figure before the Tilapia mossambica adopting embodiments of the invention to feed;
Fig. 2 is intestinal epithelial cell microscope figure before the Tilapia mossambica adopting commercially available certain commercial feed A to feed;
Fig. 3 is intestinal epithelial cell microscope figure before the Tilapia mossambica adopting commercially available certain commercial feed B to feed.
Detailed description of the invention
Below in conjunction with detailed description of the invention, technical scheme of the present invention is described in further detail, but does not form any limitation of the invention.
Embodiment 1
A kind of mixed feed improving Tilapia mossambica intestinal health of the present invention, is processed by the basic components of following quality proportioning and Chinese herbal medicine compound:
Formula 1: basic components 995kg, Chinese herbal medicine compound 5kg;
Formula 2: basic components 996kg, Chinese herbal medicine compound 4kg;
Formula 3: basic components 997kg, Chinese herbal medicine compound 3kg;
Formula 4: basic components 998kg, Chinese herbal medicine compound 2kg;
Formula 5: basic components 999kg, Chinese herbal medicine compound 1kg.
Wherein, every 1000kg basic components is made up of the raw material of following weight parts: wheat 120kg, Strong flour 80kg, dish dregs of rice 60kg, cotton dregs 80kg, dregs of beans 380kg, fish meal 50kg, soyabean lecithin oil 20kg, soybean oil 25kg, DDGS100kg, cuttlefish cream 25kg, fine bran 17.4kg, calcium dihydrogen phosphate 18kg, Choline Chloride 0.6kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish vitaminF 21V3kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish microelement pre-mix material F29M20kg, compound probiotic 0.4kg, mould inhibitor 0.3kg, antioxidant 0.3kg.In compound probiotic, the content of bacillus subtilis is 200 × 10
9cFU/g, the content of lactobacterium acidophilus is 5.0 × 10
9cFU/g;
Chinese herbal medicine compound is made up of the raw material of following weight parts: pseudo-ginseng 8g, fruit of Chinese magnoliavine 15g, Radix Astragali 7g, Radix Glycyrrhizae 12g, pseudo-ginseng 6g, Asian puccoon 16g, milk thistle 12g, Psoralea corylifolia 5g.
Its preparation method:
(1) basic components and each component of Chinese herbal medicine compound is taken by above-mentioned components by weight percent;
(2) Chinese herbal medicine extract is prepared, take in Chinese herbal medicine extract component to step (1) and first soak 30min, every 50g Chinese herbal medicine extract adds 3.0L water, and 40min is boiled in big fire (120 DEG C), little fire (70 DEG C) boils 20min, pours out liquid cooling; Add water 2.5L again in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, then the 1.5L that adds water in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, three the liquid mixing in front and back, Chinese herbal medicine extract finished product 5kg processed;
(3) this invention Feed Manufacturing is with the production technology pulverized afterwards of first preparing burden, Chinese herbal medicine compound step (2) prepared is when feed second time mixes, made an addition in basic components by interior spraying adding technology, through mixing in 140 seconds, after feed extrusion is granulated, 80 DEG C of hot-air seasonings, cooling gets product naturally---a kind of mixed feed improving Tilapia mossambica intestinal health.
Embodiment 2
Another kind provided by the invention can improve the mixed feed of Tilapia mossambica intestinal health, is processed by the basic components of following quality proportioning and Chinese herbal medicine compound:
Formula 1: basic components 995kg, Chinese herbal medicine compound 5kg;
Formula 2: basic components 998kg, Chinese herbal medicine compound 2kg;
Formula 3: basic components 997kg, Chinese herbal medicine compound 3kg;
Formula 4: basic components 999kg, Chinese herbal medicine compound 1kg;
Formula 5: basic components 996kg, Chinese herbal medicine compound 4kg.
Wherein: every 1000kg basic components is made up of the raw material of following weight parts: wheat 60kg, Strong flour 140kg, dish dregs of rice 70kg, cotton dregs 100kg, dregs of beans 310kg, fish meal 80kg, soyabean lecithin oil 10kg, soybean oil 35kg, DDGS120kg, cuttlefish cream 15kg, fine bran 19.8kg, calcium dihydrogen phosphate 20kg, Choline Chloride 0.5kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish vitaminF 21V2.5kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish microelement pre-mix material F29M15kg, compound probiotic 0.6kg, mould inhibitor 0.3kg, antioxidant 0.3kg.In compound probiotic, the content of bacillus subtilis is 700 × 10
9cFU/g, the content of lactobacterium acidophilus is 2.0 × 10
9cFU/g;
Chinese herbal medicine compound is made up of the raw material of following weight parts: pseudo-ginseng 4g, fruit of Chinese magnoliavine 15g, Radix Astragali 10g, Radix Glycyrrhizae 15g, pseudo-ginseng 6g, Asian puccoon 18g, milk thistle 8g, Psoralea corylifolia 10g.
Its preparation method:
(1) basic components and each component of Chinese herbal medicine compound is taken by above-mentioned components by weight percent;
(2) Chinese herbal medicine extract is prepared, take in Chinese herbal medicine extract component to step (1) and first soak 30min, every 50g Chinese herbal medicine extract adds 2.5L water, and 40min is boiled in big fire (120 DEG C), little fire (70 DEG C) boils 20min, pours out liquid cooling; Add water 2.5L again in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, then the 2.0L that adds water in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, three the liquid mixing in front and back, Chinese herbal medicine extract finished product 5kg processed;
(3) this invention Feed Manufacturing is with the production technology pulverized afterwards of first preparing burden, Chinese herbal medicine compound step (2) prepared is when feed second time mixes, made an addition in basic components by interior spraying adding technology, mixed through 120 second second, after feed extrusion is granulated, 80 DEG C of hot-air seasonings, cooling gets product naturally---a kind of mixed feed improving Tilapia mossambica intestinal health.
Embodiment 3
The present invention also provides a kind of mixed feed improving Tilapia mossambica intestinal health to be processed by 996kg basic components and 4kg Chinese herbal medicine compound.
Every 1000kg basic components is made up of the raw material of following weight parts: wheat 80kg, Strong flour 120kg, dish dregs of rice 80kg, cotton dregs 50kg, dregs of beans 340kg, fish meal 60kg, soyabean lecithin oil 15kg, soybean oil 30kg, DDGS150kg, cuttlefish cream 20kg, fine bran 15.1kg, calcium dihydrogen phosphate 15kg, Choline Chloride 0.8kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish vitaminF 21V3kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish microelement pre-mix material F29M20kg, compound probiotic 0.5kg, mould inhibitor 0.3kg, antioxidant 0.3kg.In compound probiotic, the content of bacillus subtilis is 500 × 10
9cFU/g, the content of lactobacterium acidophilus is 9.0 × 10
9cFU/g;
Described Chinese herbal medicine compound is made up of the raw material of following weight parts: pseudo-ginseng 6g, fruit of Chinese magnoliavine 10g, Radix Astragali 15g, Radix Glycyrrhizae 10g, pseudo-ginseng 8g, Asian puccoon 12g, milk thistle 10g, Psoralea corylifolia 6g.
Its preparation method:
(1) basic components and each component of Chinese herbal medicine compound is taken by above-mentioned components by weight percent;
(2) Chinese herbal medicine extract is prepared, take in Chinese herbal medicine extract component to step (1) and first soak 30min, every 50g Chinese herbal medicine extract adds 1.5L water, and 40min is boiled in big fire (120 DEG C), little fire (70 DEG C) boils 20min, pours out liquid cooling; Add water 2.5L again in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, then the 2.5L that adds water in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, three the liquid mixing in front and back, Chinese herbal medicine extract finished product 4kg processed;
(3) this invention Feed Manufacturing is with the production technology pulverized afterwards of first preparing burden, Chinese herbal medicine compound step (2) prepared is when feed second time mixes, made an addition in basic components by interior spraying adding technology, mixed through 150 second second, after feed extrusion is granulated, 80 DEG C of hot-air seasonings, cooling gets product naturally---a kind of mixed feed improving Tilapia mossambica intestinal health.
Embodiment 4
Another kind provided by the invention improves the mixed feed of Tilapia mossambica intestinal health, is processed by the basic components of following quality proportioning and Chinese herbal medicine extract:
Formula 1: basic components 995kg, Chinese herbal medicine extract 5kg;
Formula 2: basic components 998kg, Chinese herbal medicine extract 2kg;
Formula 3: basic components 997kg, Chinese herbal medicine extract 3kg;
Formula 4: basic components 999kg, Chinese herbal medicine extract 1kg;
Formula 5: basic components 996kg, Chinese herbal medicine extract 4kg.
Wherein: every 1000kg basic components is made up of the raw material of following weight parts: wheat 60kg, Strong flour 140kg, dish dregs of rice 70kg, cotton dregs 100kg, dregs of beans 310kg, fish meal 80kg, soyabean lecithin oil 10kg, soybean oil 35kg, DDGS120kg, cuttlefish cream 15kg, fine bran 19.8kg, calcium dihydrogen phosphate 20kg, Choline Chloride 0.5kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish vitaminF 21V2.5kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish microelement pre-mix material F29M15kg, compound probiotic 0.6kg, mould inhibitor 0.3kg, antioxidant 0.3kg.In compound probiotic, the content of bacillus subtilis is 300 × 10
9cFU/g, the content of lactobacterium acidophilus is 7.0 × 10
9cFU/g;
Chinese herbal medicine extract is made up of the raw material of following weight parts: pseudo-ginseng 10g, fruit of Chinese magnoliavine 15g, Radix Astragali 20g, Radix Glycyrrhizae 15g, pseudo-ginseng 10g, Asian puccoon 18g, milk thistle 15g, Psoralea corylifolia 10g.
Its preparation method:
(1) basic components and each component of Chinese herbal medicine extract is taken by above-mentioned components by weight percent;
(2) Chinese herbal medicine extract is prepared, take in Chinese herbal medicine extract component to step (1) and first soak 30min, every 50g Chinese herbal medicine extract adds 2.5L water, and 40min is boiled in big fire (120 DEG C), little fire (70 DEG C) boils 20min, pours out liquid cooling; Add water 2.5L again in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, then the 2.0L that adds water in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, after three the liquid mixing in front and back, remove excessive moisture, Chinese herbal medicine extract finished product 1kg processed
Adopt said herbal medicine extract 4 parts, more parallelly produce 4 times, altogether obtain 5kg Chinese herbal medicine extract.
(3) this invention Feed Manufacturing is with the production technology pulverized afterwards of first preparing burden, Chinese herbal medicine extract step (2) prepared is when feed second time mixes, made an addition in basic components by interior spraying adding technology, mixed through 120 second second, after feed extrusion is granulated, 80 DEG C of hot-air seasonings, cooling gets product naturally---a kind of mixed feed improving Tilapia mossambica intestinal health.
Embodiment 5
A kind of mixed feed improving Tilapia mossambica intestinal health of the present invention, is processed by the basic components of following quality proportioning and Chinese herbal medicine extract:
Formula 1: basic components 995kg, Chinese herbal medicine extract 5kg;
Formula 2: basic components 996kg, Chinese herbal medicine extract 4kg;
Formula 3: basic components 997kg, Chinese herbal medicine extract 3kg;
Formula 4: basic components 998kg, Chinese herbal medicine extract 2kg;
Formula 5: basic components 999kg, Chinese herbal medicine extract 1kg.
Wherein, every 1000kg basic components is made up of the raw material of following weight parts: wheat 120kg, Strong flour 80kg, dish dregs of rice 60kg, cotton dregs 80kg, dregs of beans 380kg, fish meal 50kg, soyabean lecithin oil 20kg, soybean oil 25kg, DDGS100kg, cuttlefish cream 25kg, fine bran 17.4kg, calcium dihydrogen phosphate 18kg, Choline Chloride 0.6kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish vitaminF 21V3kg, Guangdong Tongwei Feed Co., Ltd.'s omnivorefish microelement pre-mix material F29M20kg, compound probiotic 0.4kg, mould inhibitor 0.3kg, antioxidant 0.3kg.In compound probiotic, the content of bacillus subtilis is 250 × 10
9cFU/g, the content of lactobacterium acidophilus is 5.5 × 10
9cFU/g;
Chinese herbal medicine extract is made up of the raw material of following weight parts: pseudo-ginseng 2g, fruit of Chinese magnoliavine 3g, Radix Astragali 5g, Radix Glycyrrhizae 5g, pseudo-ginseng 2g, Asian puccoon 6g, milk thistle 5, Psoralea corylifolia 2g.
Its preparation method:
(1) basic components and each component of Chinese herbal medicine extract is taken by above-mentioned components by weight percent;
(2) Chinese herbal medicine extract is prepared, take in Chinese herbal medicine extract component to step (1) and first soak 30min, every 50g Chinese herbal medicine extract adds 3.0L water, and 40min is boiled in big fire (120 DEG C), little fire (70 DEG C) boils 20min, pours out liquid cooling; Add water 2.5L again in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, then the 1.5L that adds water in medicine, 10min is boiled in big fire (120 DEG C), little fire (60 DEG C) boils 20min, pour out liquid cooling, three the liquid mixing in front and back, Chinese herbal medicine extract finished product 5kg processed;
(3) this invention Feed Manufacturing is with the production technology pulverized afterwards of first preparing burden, Chinese herbal medicine extract step (2) prepared is when feed second time mixes, made an addition in basic components by interior spraying adding technology, through mixing in 140 seconds, after feed extrusion is granulated, 80 DEG C of hot-air seasonings, cooling gets product naturally---a kind of mixed feed improving Tilapia mossambica intestinal health.
In order to effect of the present invention is better described, provide the mixed feed of the improved Tilapia mossambica intestinal health provided in embodiment 3 below to Growth Op Tilapia culture experiment.
In order to illustrate that the present invention contains the mixed feed of the improved Tilapia mossambica intestinal health of medicinal herb components to the impact of Growth Op Tilapia performance, efficiency of feed utilization, intestinal tissue structure and non-specific immunity, Tilapia mossambica culture experiment carried out by the net cage that the present invention uses length, width and height to be 1.5m.3 processed group groups are established in test altogether, each processed group establishes 3 repetitions, each Tilapia mossambica 40 tail repeating to put in a suitable place to breed initial counterpoise and be about 8.8g/ tail, the mixed feed of the embodiment of the present invention of throwing something and feeding respectively 3, commercially available certain commercial feed A, commercially available certain commercial feed B, and be designated as G0, G1, G2 respectively.Test cultivation 2 months by a definite date, every day, throwing was raised 2 times (8:30,17:30), and throwing the rate of raising is 3% of its body weight.Cultivation is thrown something and fed after off-test, and stop eating 1 day, each net cage is weighed respectively and added up fish number.5 tail fishes got at random by each net cage, and on ice pan, solution takes its anterior intestine (near middle intestinal segment), is stored in the centrifuge tube with 10ml, for foregut tissues structure observation with 10% formalin solution.5 tail fishes got at random by each net cage, and solution takes its anterior intestine, for subsequent use in-80 DEG C of Refrigerator stores after mixing, detect for enteron aisle salivary lysozyme.Coherent detection and analysis result as follows:
The impact on Growth Op Tilapia performance is expected in table 1 the present invention and commercial goods
The impact on Tilapia mossambica intestinal health index is expected in table 2 the present invention and commercial goods
Note: the shoulder mark variant conspicuousness of different letter representation (P < 0.05) in data of going together in table 1,2.
As can be seen from Table 1, this technological invention comparatively commercial goods feed A, B cultivating tilapia is compared for 2 months, and this technological invention can significantly improve whole opisthosoma weight, rate of body weight gain, the specific growth rate of Tilapia mossambica, significantly can reduce the feed coefficient of Tilapia mossambica.As can be seen from Table 2; this technological invention comparatively commercial goods feed A, B cultivating tilapia is compared for 2 months, and this technological invention can significantly improve Tilapia mossambica enteron aisle nonspecific mental retardation and oxidation resistance, safeguard that intestinal beneficial flora is stable, protection Tilapia mossambica intestinal health.
As Fig. 1 to Fig. 3, the corresponding G2 of Fig. 1 corresponding G0, Fig. 2 corresponding G1, Fig. 3.As can be seen from Figure 1, this technological invention compared with commercial goods feed A, B cultivating tilapia 2 months, this technological invention cultivation Tilapia mossambica before intestinal epithelial cell marshalling, striated border is flourishing, goblet cell is more, and tunica propria connective tissue is normal, does not find any pathology damage; Intestinal epithelial cell vacuolar degeneration before feed A, B cultivating tilapia of commercial goods, part striated border comes off, and pathology damage symptom, appears in goblet epithelium hyperplasia, tunica propria connective tissue loose.
Above-mentioned test proves, the present invention can improve the mixed feed of Tilapia mossambica intestinal health in promotion different phase Growth Op Tilapia performance, aspects such as improving feed efficiency and non-specific immunity, improve Tilapia mossambica intestinal health, safeguard that Tilapia mossambica gut flora is stablized all obtains good effect.Can well be applied.
Above-describedly be only preferred embodiment of the present invention, all do within the scope of the spirit and principles in the present invention any amendment, equivalently to replace and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. can improve a mixed feed for Tilapia mossambica intestinal health, it is characterized in that, described mixed feed is obtained after combination drying by Chinese herbal medicine extract and basic components; Described basic components and the part by weight of Chinese herbal medicine extract are 995 ~ 999:1 ~ 5;
Every 1 ~ 5kg Chinese herbal medicine extract is obtained by following Chinese herbal medicine water extraction:
Pseudo-ginseng 2 ~ 10g, the fruit of Chinese magnoliavine 3 ~ 15g, the Radix Astragali 5 ~ 20g, Radix Glycyrrhizae 5 ~ 15g, pseudo-ginseng 2 ~ 10g, Asian puccoon 6 ~ 18g, milk thistle 5 ~ 15g, Psoralea corylifolia 2 ~ 10g.
2. the mixed feed improving Tilapia mossambica intestinal health according to claim 1, is characterized in that: every 1 ~ 5kg Chinese herbal medicine extract is obtained by following Chinese herbal medicine water extraction: pseudo-ginseng 4 ~ 8g, the fruit of Chinese magnoliavine 10 ~ 15g, the Radix Astragali 7 ~ 15g, Radix Glycyrrhizae 10 ~ 15g, pseudo-ginseng 6 ~ 8g, Asian puccoon 12 ~ 18g, milk thistle 8 ~ 12g, Psoralea corylifolia 5 ~ 10g.
3. the mixed feed improving Tilapia mossambica intestinal health according to claim 1, is characterized in that: the basic components of every 100 weight portions comprises following parts by weight of component:
Wheat 3.0 ~ 13.0 parts, Strong flour 6.0 ~ 16.0 parts, the dish dregs of rice 5.0 ~ 15.0 parts, cotton dregs 3.0 ~ 12.0 parts, dregs of beans 15.0 ~ 40.0 parts, fish meal 2.0 ~ 10.0 parts, soyabean lecithin oil 0.5 ~ 3.0 part, soybean oil 2.0 ~ 5.0 parts, import DDGS8.0 ~ 20.0 part, cuttlefish cream 1.0 ~ 4.0 parts, fine bran 0.5 ~ 3.0 part, calcium dihydrogen phosphate 1 ~ 3 part, Choline Chloride 0.05 ~ 0.1 part, mineral substance premix 1 ~ 3 part, vitamin premix 0.1 ~ 0.5 part, compound probiotic 0.03 ~ 0.07 part.
4. the mixed feed improving Tilapia mossambica intestinal health according to claim 3, it is characterized in that: the basic components of every 100 weight portions comprises following parts by weight of component: wheat 6.0 ~ 12.0 parts, Strong flour 8.0 ~ 14.0 parts, the dish dregs of rice 6.0 ~ 8.0 parts, cotton dregs 5.0 ~ 10.0 parts, dregs of beans 31.0 ~ 38.0 parts, fish meal 5.0 ~ 8.0 parts, soyabean lecithin oil 1 ~ 2.0 part, soybean oil 2.5 ~ 3.5 parts, import DDGS10.0 ~ 15.0 part, cuttlefish cream 1.5 ~ 2.5 parts, fine bran 1.5 ~ 2.0 parts, calcium dihydrogen phosphate 1.5 ~ 2 parts, Choline Chloride 0.05 ~ 0.08 part, mineral substance premix 1.5 ~ 2 parts, vitamin premix 0.25 ~ 0.3 part, compound probiotic 0.04 ~ 0.06 part.
5. the mixed feed of the improved Tilapia mossambica intestinal health according to claim 3 or 4, is characterized in that: described basic components also comprises mould inhibitor 0.03 part, antioxidant 0.03 part.
6. the mixed feed of the improved Tilapia mossambica intestinal health according to claim 3 or 4, is characterized in that: described vitamin premix is the omnivorefish vitamin premix F21V of Guangdong Tongwei Feed Co., Ltd.; Described mineral substance premix is the omnivorefish microelement pre-mix material F29M of Guangdong Tongwei Feed Co., Ltd.; Described compound probiotic main component is bacillus subtilis, and its specification is 100 ~ 800 × 10
9cFU/g, lactobacterium acidophilus, its specification is 1 ~ 10.0 × 10
9cFU/g.
7. a preparation method for the mixed feed of the improved Tilapia mossambica intestinal health as described in as arbitrary in claim 1 to 6, is characterized in that: comprise the steps
Step 1: take Chinese herbal medicine according to components by weight percent and carry out water extraction, and take basic components carry out first time mixing;
Step 2: basic components carried out second time mixing and adopt interior spraying adding technology to add in basic components by Chinese herbal medicine extract in the process;
Step 3: mixed for step 2 material is carried out extrusion granulation, and dry, cool.
8. the preparation method improving the mixed feed of Tilapia mossambica intestinal health according to claim 7, is characterized in that: in described step 2, after Chinese herbal medicine extract adds basic components to, mixes 120 ~ 180 seconds.
9. the preparation method improving the mixed feed of Tilapia mossambica intestinal health according to claim 7, is characterized in that: in described step 3, adopts the hot blast of 80 ~ 90 DEG C to carry out drying.
10. the preparation method improving the mixed feed of Tilapia mossambica intestinal health according to claim 7, is characterized in that: the method for described Chinese herbal medicine extract water extraction is as follows:
Component takes Chinese herbal medicine by weight, and soaks 20 ~ 40min, adds 1.0 ~ 4.0L water, boil 30min ~ 60min in every 50g Chinese herbal medicine, pours out liquid cooling; Add water 1.0 ~ 3.0L again in Chinese herbal medicine, boils 20min ~ 60min, pours out liquid cooling, then the 1.0 ~ 3.0L that adds water in medicine, boils 20min-60min, pours out liquid cooling, three the liquid mixing in front and back, Chinese herbal medicine extract 1.0 ~ 5.0kg processed.
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| CN106615993A (en) * | 2016-09-13 | 2017-05-10 | 通威股份有限公司 | Compound feed used for tilapia mossambica breeding and preparation method thereof |
| CN106954761A (en) * | 2017-03-30 | 2017-07-18 | 广州市海维饲料有限公司 | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof |
| CN108077125A (en) * | 2017-12-26 | 2018-05-29 | 广西金海环岛渔业有限公司 | Improve the cultural method of Tilapia mossambica oxidation resistance |
| CN110840963A (en) * | 2019-09-27 | 2020-02-28 | 珠海容川饲料有限公司 | Chinese herbal medicine additive and artificial mixed feed for improving intestinal health of loaches and preparation method thereof |
| CN112167120A (en) * | 2020-10-14 | 2021-01-05 | 中国水产科学研究院淡水渔业研究中心 | Breeding method for improving intestinal immunity of tilapia |
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| CN114470084A (en) * | 2022-01-06 | 2022-05-13 | 佛山市顺德区旺海饲料实业有限公司 | Environment-friendly and efficient composition for preventing and treating white feces of tilapia mossambica as well as preparation method and application thereof |
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