CN103750072A - Green pig feed additive capable of substituting antibiotic - Google Patents
Green pig feed additive capable of substituting antibiotic Download PDFInfo
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- CN103750072A CN103750072A CN201410054559.2A CN201410054559A CN103750072A CN 103750072 A CN103750072 A CN 103750072A CN 201410054559 A CN201410054559 A CN 201410054559A CN 103750072 A CN103750072 A CN 103750072A
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Abstract
The invention discloses a green pig feed additive capable of substituting antibiotic, and belongs to the field of feed additives. The additive comprises fermented Chinese herbal medicine powder, a rhodiola rosea extract, folium artemisiae argyi powder, radix glehniae powder, folium cortex eucommiae powder, a honeysuckle stem, centella asiatica powder, platycodon grandiflorum powder and dandelion powder. The additive has the characteristics of no toxicity, no residue and no tolerance, can effectively improve the immunity of a pig, can improve the disease resistance of the pig, can reduce the incidence of the pig, can improve the growth property of the pig, can increase a breeding benefit, can substitute the antibiotic in a daily ration of the pug, and is broad in application prospect.
Description
Technical field
The present invention relates to a kind of pig's feed additive, especially relate to a kind of alternative antibiotic pig green feed additive, belong to feed additive field.
Technical background
Feed safety is the significant problem of world today's extensive concern, and quality of the fodder direct relation the quality of cultured product, and feed addictive is the key component of feed.Antibiotics feed was used in a large number in the past, but along with people are to food security and healthy attention, find that antibiotics feed exists many drawbacks, the long-term use of antibiotic in feed causes the generation of drug resistance, cause livestock and poultry endogenous infection and suprainfection, immunity of livestock is declined, residual etc. in animal products and environment.This also becomes a global difficult problem, increasing developed country starts to limit the use of or forbid feeding antibiotic, China has also limited part antibiotic and has used in feed as feed addictive, there is clearly regulation in country to antibiotic use in additive: the antibiotic 1, banning use of, as chloramphenicol; 2, the antibiotic that limitation is used, as terramycin.Therefore, the green feed additive of development of new safety, substitutes or part substitute antibiotics or the application of medicine in livestock and poultry breeding industry, for alleviating feed and the food-safety problems such as livestock products drug residue and pathogenic bacteria resistance to drugs, ensure human health, there is positive meaning.
Chinese herbal medicine is as the rarity of China, because the physiological function of its low toxic and side effect, basic noresidue, nothing " three cause ", the uniqueness such as free from environmental pollution becomes the focus that researchers pay close attention to.But the application of Chinese herbal feed additive in livestock and poultry at present also exists some problems, tradition application mostly is Chinese herbal medicine and directly pulverizes, and formulation is single, addition is large, effect is unstable.
So develop a kind of green additive take Chinese herbal medicine as main component, carry out the application in feed such as substitute antibiotics, nontoxic, noresidue, have no drug resistance, meet the needs of social development, simultaneously the sustainable health development important in inhibiting to animal husbandry.
Summary of the invention
The object of the present invention is to provide a kind of nontoxic, noresidue, have no drug resistance, antibiotic green feed additive in alternative daily ration, this additive can effectively improve the immunity of pig, strengthen the resistance against diseases of pig, reduce the incidence of disease of pig, can also improve the growth performance of pig, increase culture benefit simultaneously.
For achieving the above object, the present invention adopts following technical scheme:
A kind of alternative antibiotic pig green feed additive, is comprised of following raw materials by weight portion: fermentation of Chinese herbal medicine powder 10-15 part, gadol extract 1-3 part, Folium Artemisiae Argyi powder 4-8 part, radix glehniae powder 3-6 part, Eucommia Leaf Powder 2-6 part, caulis lonicerae powder 2-8 part, accumulated snow grass meal 3-8 part, balloonflower powder 2-6 part, dandelion powder 1-4 part.
The preparation method of described fermentation of Chinese herbal medicine powder is:
(1) by selected cattail pollen, rascal, the vine of multiflower knotweed, tamarind, the fruit of Chinese magnoliavine and mother chrysanthemum in mass ratio 2-3:1-4:2-5:1-3:2-4:2-5 co-grinding cross 30 mesh sieves, with 75% the ethanol spraying sterilizing 30-60 minute that evenly carries out disinfection, through 55 ℃ of oven dry, obtain mixing Chinese herbal medicine powder;
(2) mixing Chinese herbal medicine powder and composite fermentation liquid are mixed by solid-to-liquid ratio 100:1-3, must contain bacterium Chinese herbal medicine powder; Described composite fermentation liquid is in every 100ml composite fermentation liquid, to contain bacillus licheniformis 25ml, bacillus subtilis 35ml, bifidobacterium breve 18 ml, Rhodopseudomonas palustris 22 ml, and wherein the viable count of bacillus licheniformis is 2.4 × 10
8cfu/ml, the viable count of bacillus subtilis is 1.8 × 10
9the viable count of cfu/ml, bifidobacterium breve is 2.1 × 10
8the viable count of cfu/ml, Rhodopseudomonas palustris is 1.6 × 10
7cfu/ml;
(3) will, containing bacterium Chinese herbal medicine powder and fermentation medium 48:2-3 in mass ratio, mix, then add sterilized water by solid-to-liquid ratio 1:1, fermentation 48-72h, obtains fermentation of Chinese herbal medicine powder, by fermentation of Chinese herbal medicine powder drying and crushing, crosses 60 mesh sieves; Described fermentation medium is by weight being comprised of 0.1 part of 8 parts of glucose, 5 parts of peptones, 5 parts of beef extracts, 0.3 part, sodium chloride, 0.2 part, magnesium sulfate, manganese sulfate;
The preparation method of described gadol extract is: rhodiola root was pulverized to 40 mesh sieves, by rhodiola root powder and complex enzyme in mass ratio 100:1-3 mix, by solid-to-liquid ratio 1:3, add distilled water again, heating maintains 48 ℃ by water temperature, carry out enzymolysis, enzymolysis time 1-2h, in enzymolysis process, every 10 minutes stir 1 time, after enzymolysis is complete by dry rhodiola root dried bean noodles; The ethanol of rhodiola root powder after enzymolysis and 60-75% is mixed by solid-to-liquid ratio 1:5-8, and ultrasonic extraction 30-40 minute, filters, and filtrate drying and crushing is crossed to 60 mesh sieves, obtains gadol extract; Described complex enzyme is cellulase and pectase 3:2 composition in mass ratio.
The preparation method of described Folium Artemisiae Argyi powder is: tarragon was pulverized to 50 mesh sieves;
The preparation method of described radix glehniae powder is: radix glehniae was pulverized to 50 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae was pulverized to 50 mesh sieves;
The preparation method of described caulis lonicerae powder is: caulis lonicerae was pulverized to 50 mesh sieves;
The preparation method of described accumulated snow grass meal is: centella was pulverized to 50 mesh sieves;
The preparation method of described balloonflower powder is: balloonflower root was pulverized to 50 mesh sieves;
The preparation method of described dandelion powder is: dandelion was pulverized to 50 mesh sieves.
A kind of alternative antibiotic pig green feed additive in the present invention, its preparation method is as follows:
Step 1: Folium Artemisiae Argyi powder, radix glehniae powder, Eucommia Leaf Powder, caulis lonicerae powder, accumulated snow grass meal, balloonflower powder and dandelion powder are mixed by formula rate, by solid-to-liquid ratio, 1:4-8 adds distilled water, the 1-2h that refluxes under 50-60 ℃ of condition, refluxes and finishes rear drying and crushing and cross 60 mesh sieves, stand-by;
Step 2: step 1 is made to thing and fermentation of Chinese herbal medicine powder and gadol extract and mix and obtain additive of the present invention.
A kind of alternative antibiotic pig green feed additive in the present invention, the addition in pig feed is 600-1000 gram/ton.
Beneficial effect
The present invention is composite by composition science such as fermentation of Chinese herbal medicine powder, gadol extract, Folium Artemisiae Argyi powder, radix glehniae powder, can obtain a kind of nontoxic, noresidue, have no drug resistance, antibiotic green feed additive in alternative daily ration, this additive can not only effectively improve the immunity of pig, strengthen the resistance against diseases of pig, reduce the incidence of disease of pig, can also improve the growth performance of pig, increase culture benefit simultaneously.The present invention has also adopted biofermentation technique in addition, and Chinese herbal medicine is fermented, and makes Chinese herbal medicine and probio realize organic assembling, not only improved Chinese herbal medicine pharmacologically active, improve its result of use, probio more can effectively be improved the intestinal health of pig, regulates metabolic function.The formula components of product of the present invention is natural products simultaneously, and green safety, meets social development, to the sustainable health development important in inhibiting of animal husbandry.
Specific embodiment
Embodiment 1
A kind of alternative antibiotic pig green feed additive, is comprised of following raw materials by weight portion: 10 parts, fermentation of Chinese herbal medicine powder, 1 part of gadol extract, 4 parts of Folium Artemisiae Argyi powders, 3 parts, radix glehniae powder, 2 parts of Eucommia Leaf Powders, 2 parts, caulis lonicerae powder, 3 parts of accumulated snow grass meals, 2 parts of balloonflower powders, 1 part, dandelion powder.
The preparation method of described fermentation of Chinese herbal medicine powder is:
(1) by selected cattail pollen, rascal, the vine of multiflower knotweed, tamarind, the fruit of Chinese magnoliavine and mother chrysanthemum in mass ratio 2:1:2:1:2:2 co-grinding cross 30 mesh sieves, with 75% the ethanol spraying sterilizing 30 minutes that evenly carries out disinfection, through 55 ℃ of oven dry, obtain mixing Chinese herbal medicine powder;
(2) mixing Chinese herbal medicine powder and composite fermentation liquid are mixed by solid-to-liquid ratio 100:1, must contain bacterium Chinese herbal medicine powder; Described composite fermentation liquid is in every 100ml composite fermentation liquid, to contain bacillus licheniformis 25ml, bacillus subtilis 35ml, bifidobacterium breve 18 ml, Rhodopseudomonas palustris 22 ml, and wherein the viable count of bacillus licheniformis is 2.4 × 10
8cfu/ml, the viable count of bacillus subtilis is 1.8 × 10
9the viable count of cfu/ml, bifidobacterium breve is 2.1 × 10
8the viable count of cfu/ml, Rhodopseudomonas palustris is 1.6 × 10
7cfu/ml;
(3) will, containing bacterium Chinese herbal medicine powder and fermentation medium 48:2 in mass ratio, mix, then add sterilized water by solid-to-liquid ratio 1:1, fermentation 48h, obtains fermentation of Chinese herbal medicine powder, by fermentation of Chinese herbal medicine powder drying and crushing, crosses 60 mesh sieves; Described fermentation medium is by weight being comprised of 0.1 part of 8 parts of glucose, 5 parts of peptones, 5 parts of beef extracts, 0.3 part, sodium chloride, 0.2 part, magnesium sulfate, manganese sulfate;
The preparation method of described gadol extract is: rhodiola root was pulverized to 40 mesh sieves, by rhodiola root powder and complex enzyme in mass ratio 100:1 mix, by solid-to-liquid ratio 1:3, add distilled water again, heating maintains 48 ℃ by water temperature, carry out enzymolysis, enzymolysis time 1h, in enzymolysis process, every 10 minutes stir 1 time, after enzymolysis is complete by dry rhodiola root dried bean noodles; The ethanol of the rhodiola root powder after enzymolysis and 60% is mixed by solid-to-liquid ratio 1:5, and ultrasonic extraction 30 minutes, filters, and filtrate drying and crushing is crossed to 60 mesh sieves, obtains gadol extract; Described complex enzyme is cellulase and pectase 3:2 composition in mass ratio.
The preparation method of described Folium Artemisiae Argyi powder is: tarragon was pulverized to 50 mesh sieves;
The preparation method of described radix glehniae powder is: radix glehniae was pulverized to 50 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae was pulverized to 50 mesh sieves;
The preparation method of described caulis lonicerae powder is: caulis lonicerae was pulverized to 50 mesh sieves;
The preparation method of described accumulated snow grass meal is: centella was pulverized to 50 mesh sieves;
The preparation method of described balloonflower powder is: balloonflower root was pulverized to 50 mesh sieves;
The preparation method of described dandelion powder is: dandelion was pulverized to 50 mesh sieves.
A kind of alternative antibiotic pig green feed additive in the present invention, its preparation method is as follows:
Step 1: Folium Artemisiae Argyi powder, radix glehniae powder, Eucommia Leaf Powder, caulis lonicerae powder, accumulated snow grass meal, balloonflower powder and dandelion powder are mixed by formula rate, by solid-to-liquid ratio, 1:4 adds distilled water, the 1h that refluxes under 50 ℃ of conditions, refluxes and finishes rear drying and crushing and cross 60 mesh sieves, stand-by;
Step 2: step 1 is made to thing and fermentation of Chinese herbal medicine powder and gadol extract and mix and obtain additive of the present invention.
A kind of alternative antibiotic pig green feed additive in the present invention, the addition in pig feed is 600 grams/ton.
Embodiment 2
A kind of alternative antibiotic pig green feed additive, is comprised of following raw materials by weight portion: 13 parts, fermentation of Chinese herbal medicine powder, 2 parts of gadol extracts, 6 parts of Folium Artemisiae Argyi powders, 5 parts, radix glehniae powder, 4 parts of Eucommia Leaf Powders, 5 parts, caulis lonicerae powder, 6 parts of accumulated snow grass meals, 4 parts of balloonflower powders, 3 parts, dandelion powder.
The preparation method of described fermentation of Chinese herbal medicine powder is:
(1) by selected cattail pollen, rascal, the vine of multiflower knotweed, tamarind, the fruit of Chinese magnoliavine and mother chrysanthemum in mass ratio 3:2:4:2:3:4 co-grinding cross 30 mesh sieves, with 75% the ethanol spraying sterilizing 45 minutes that evenly carries out disinfection, through 55 ℃ of oven dry, obtain mixing Chinese herbal medicine powder;
(2) mixing Chinese herbal medicine powder and composite fermentation liquid are mixed by solid-to-liquid ratio 100:2, must contain bacterium Chinese herbal medicine powder; Described composite fermentation liquid is in every 100ml composite fermentation liquid, to contain bacillus licheniformis 25ml, bacillus subtilis 35ml, bifidobacterium breve 18 ml, Rhodopseudomonas palustris 22 ml, and wherein the viable count of bacillus licheniformis is 2.4 × 10
8cfu/ml, the viable count of bacillus subtilis is 1.8 × 10
9the viable count of cfu/ml, bifidobacterium breve is 2.1 × 10
8the viable count of cfu/ml, Rhodopseudomonas palustris is 1.6 × 10
7cfu/ml;
(3) will, containing bacterium Chinese herbal medicine powder and fermentation medium 48:3 in mass ratio, mix, then add sterilized water by solid-to-liquid ratio 1:1, fermentation 62h, obtains fermentation of Chinese herbal medicine powder, by fermentation of Chinese herbal medicine powder drying and crushing, crosses 60 mesh sieves; Described fermentation medium is by weight being comprised of 0.1 part of 8 parts of glucose, 5 parts of peptones, 5 parts of beef extracts, 0.3 part, sodium chloride, 0.2 part, magnesium sulfate, manganese sulfate;
The preparation method of described gadol extract is: rhodiola root was pulverized to 40 mesh sieves, by rhodiola root powder and complex enzyme in mass ratio 100:2 mix, by solid-to-liquid ratio 1:3, add distilled water again, heating maintains 48 ℃ by water temperature, carry out enzymolysis, enzymolysis time 1.5h, in enzymolysis process, every 10 minutes stir 1 time, after enzymolysis is complete by dry rhodiola root dried bean noodles; The ethanol of the rhodiola root powder after enzymolysis and 70% is mixed by solid-to-liquid ratio 1:7, and ultrasonic extraction 35 minutes, filters, and filtrate drying and crushing is crossed to 60 mesh sieves, obtains gadol extract; Described complex enzyme is cellulase and pectase 3:2 composition in mass ratio.
The preparation method of described Folium Artemisiae Argyi powder is: tarragon was pulverized to 50 mesh sieves;
The preparation method of described radix glehniae powder is: radix glehniae was pulverized to 50 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae was pulverized to 50 mesh sieves;
The preparation method of described caulis lonicerae powder is: caulis lonicerae was pulverized to 50 mesh sieves;
The preparation method of described accumulated snow grass meal is: centella was pulverized to 50 mesh sieves;
The preparation method of described balloonflower powder is: balloonflower root was pulverized to 50 mesh sieves;
The preparation method of described dandelion powder is: dandelion was pulverized to 50 mesh sieves.
A kind of alternative antibiotic pig green feed additive in the present invention, its preparation method is as follows:
Step 1: Folium Artemisiae Argyi powder, radix glehniae powder, Eucommia Leaf Powder, caulis lonicerae powder, accumulated snow grass meal, balloonflower powder and dandelion powder are mixed by formula rate, by solid-to-liquid ratio, 1:6 adds distilled water, the 1.5h that refluxes under 55 ℃ of conditions, refluxes and finishes rear drying and crushing and cross 60 mesh sieves, stand-by;
Step 2: step 1 is made to thing and fermentation of Chinese herbal medicine powder and gadol extract and mix and obtain additive of the present invention.
A kind of alternative antibiotic pig green feed additive in the present invention, the addition in pig feed is 800 grams/ton.
Embodiment 3
A kind of alternative antibiotic pig green feed additive, is comprised of following raw materials by weight portion: 15 parts, fermentation of Chinese herbal medicine powder, 3 parts of gadol extracts, 8 parts of Folium Artemisiae Argyi powders, 6 parts, radix glehniae powder, 6 parts of Eucommia Leaf Powders, 8 parts, caulis lonicerae powder, 8 parts of accumulated snow grass meals, 6 parts of balloonflower powders, 4 parts, dandelion powder.
The preparation method of described fermentation of Chinese herbal medicine powder is:
(1) by selected cattail pollen, rascal, the vine of multiflower knotweed, tamarind, the fruit of Chinese magnoliavine and mother chrysanthemum in mass ratio 3:4:5:3:4:5 co-grinding cross 30 mesh sieves, with 75% the ethanol spraying sterilizing 60 minutes that evenly carries out disinfection, through 55 ℃ of oven dry, obtain mixing Chinese herbal medicine powder;
(2) mixing Chinese herbal medicine powder and composite fermentation liquid are mixed by solid-to-liquid ratio 100:3, must contain bacterium Chinese herbal medicine powder; Described composite fermentation liquid is in every 100ml composite fermentation liquid, to contain bacillus licheniformis 25ml, bacillus subtilis 35ml, bifidobacterium breve 18 ml, Rhodopseudomonas palustris 22 ml, and wherein the viable count of bacillus licheniformis is 2.4 × 10
8cfu/ml, the viable count of bacillus subtilis is 1.8 × 10
9the viable count of cfu/ml, bifidobacterium breve is 2.1 × 10
8the viable count of cfu/ml, Rhodopseudomonas palustris is 1.6 × 10
7cfu/ml;
(3) will, containing bacterium Chinese herbal medicine powder and fermentation medium 48:3 in mass ratio, mix, then add sterilized water by solid-to-liquid ratio 1:1, fermentation 72h, obtains fermentation of Chinese herbal medicine powder, by fermentation of Chinese herbal medicine powder drying and crushing, crosses 60 mesh sieves; Described fermentation medium is by weight being comprised of 0.1 part of 8 parts of glucose, 5 parts of peptones, 5 parts of beef extracts, 0.3 part, sodium chloride, 0.2 part, magnesium sulfate, manganese sulfate;
The preparation method of described gadol extract is: rhodiola root was pulverized to 40 mesh sieves, by rhodiola root powder and complex enzyme in mass ratio 100:3 mix, by solid-to-liquid ratio 1:3, add distilled water again, heating maintains 48 ℃ by water temperature, carry out enzymolysis, enzymolysis time 2h, in enzymolysis process, every 10 minutes stir 1 time, after enzymolysis is complete by dry rhodiola root dried bean noodles; The ethanol of the rhodiola root powder after enzymolysis and 75% is mixed by solid-to-liquid ratio 1:8, and ultrasonic extraction 40 minutes, filters, and filtrate drying and crushing is crossed to 60 mesh sieves, obtains gadol extract; Described complex enzyme is cellulase and pectase 3:2 composition in mass ratio.
The preparation method of described Folium Artemisiae Argyi powder is: tarragon was pulverized to 50 mesh sieves;
The preparation method of described radix glehniae powder is: radix glehniae was pulverized to 50 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae was pulverized to 50 mesh sieves;
The preparation method of described caulis lonicerae powder is: caulis lonicerae was pulverized to 50 mesh sieves;
The preparation method of described accumulated snow grass meal is: centella was pulverized to 50 mesh sieves;
The preparation method of described balloonflower powder is: balloonflower root was pulverized to 50 mesh sieves;
The preparation method of described dandelion powder is: dandelion was pulverized to 50 mesh sieves.
A kind of alternative antibiotic pig green feed additive in the present invention, its preparation method is as follows:
Step 1: Folium Artemisiae Argyi powder, radix glehniae powder, Eucommia Leaf Powder, caulis lonicerae powder, accumulated snow grass meal, balloonflower powder and dandelion powder are mixed by formula rate, by solid-to-liquid ratio, 1:8 adds distilled water, the 2h that refluxes under 60 ℃ of conditions, refluxes and finishes rear drying and crushing and cross 60 mesh sieves, stand-by;
Step 2: step 1 is made to thing and fermentation of Chinese herbal medicine powder and gadol extract and mix and obtain additive of the present invention.
A kind of alternative antibiotic pig green feed additive in the present invention, the addition in pig feed is 1000 grams/ton.
Embodiment 4
A kind of alternative antibiotic pig green feed additive, is comprised of following raw materials by weight portion: 6 parts, fermentation of Chinese herbal medicine powder, 1 part of gadol extract, 1 part of Folium Artemisiae Argyi powder, 2 parts, radix glehniae powder, 8 parts of Eucommia Leaf Powders, 1 part, caulis lonicerae powder, 2 parts of accumulated snow grass meals, 1 part of balloonflower powder, 7 parts, dandelion powder.
All the other are with embodiment 2.
Embodiment 5
A kind of alternative antibiotic pig green feed additive, is comprised of following raw materials by weight portion: 13 parts, fermentation of Chinese herbal medicine powder, 2 parts of gadol extracts, 6 parts of Folium Artemisiae Argyi powders, 5 parts, radix glehniae powder, 4 parts of Eucommia Leaf Powders, 5 parts, caulis lonicerae powder, 6 parts of accumulated snow grass meals, 4 parts of balloonflower powders, 3 parts, dandelion powder.
The preparation method of described fermentation of Chinese herbal medicine powder is:
(1) by selected cattail pollen, rascal, the vine of multiflower knotweed, tamarind, the fruit of Chinese magnoliavine and mother chrysanthemum in mass ratio 1:1:1:4:5:1 co-grinding cross 30 mesh sieves, with 55% the ethanol spraying sterilizing 5 minutes that evenly carries out disinfection, through 55 ℃ of oven dry, obtain mixing Chinese herbal medicine powder;
(2) mixing Chinese herbal medicine powder and composite fermentation liquid are mixed by solid-to-liquid ratio 100:0.5, must contain bacterium Chinese herbal medicine powder; Described composite fermentation liquid is in every 100ml composite fermentation liquid, to contain bacillus licheniformis 25ml, bacillus subtilis 25ml, bifidobacterium breve 25ml, Rhodopseudomonas palustris 25ml, and wherein the viable count of bacillus licheniformis is 2.4 × 10
8cfu/ml, the viable count of bacillus subtilis is 1.8 × 10
9the viable count of cfu/ml, bifidobacterium breve is 2.1 × 10
8the viable count of cfu/ml, Rhodopseudomonas palustris is 1.6 × 10
7cfu/ml;
(3) will, containing bacterium Chinese herbal medicine powder and fermentation medium 48:1 in mass ratio, mix, then add sterilized water by solid-to-liquid ratio 1:1, fermentation 24h, obtains fermentation of Chinese herbal medicine powder, by fermentation of Chinese herbal medicine powder drying and crushing, crosses 60 mesh sieves; Described fermentation medium is by weight being comprised of 0.1 part of 8 parts of glucose, 5 parts of peptones, 5 parts of beef extracts, 0.3 part, sodium chloride, 0.2 part, magnesium sulfate, manganese sulfate;
The preparation method of described gadol extract is: rhodiola root was pulverized to 40 mesh sieves, by rhodiola root powder and complex enzyme in mass ratio 100:0.3 mix, by solid-to-liquid ratio 1:2, add distilled water again, heating maintains 48 ℃ by water temperature, carry out enzymolysis, enzymolysis time 0.5h, in enzymolysis process, every 10 minutes stir 1 time, after enzymolysis is complete by dry rhodiola root dried bean noodles; The ethanol of the rhodiola root powder after enzymolysis and 30% is mixed by solid-to-liquid ratio 1:3, and ultrasonic extraction 10 minutes, filters, and filtrate drying and crushing is crossed to 60 mesh sieves, obtains gadol extract; Described complex enzyme is cellulase and pectase 3:2 composition in mass ratio.
The preparation method of described Folium Artemisiae Argyi powder is: tarragon was pulverized to 50 mesh sieves;
The preparation method of described radix glehniae powder is: radix glehniae was pulverized to 50 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae was pulverized to 50 mesh sieves;
The preparation method of described caulis lonicerae powder is: caulis lonicerae was pulverized to 50 mesh sieves;
The preparation method of described accumulated snow grass meal is: centella was pulverized to 50 mesh sieves;
The preparation method of described balloonflower powder is: balloonflower root was pulverized to 50 mesh sieves;
The preparation method of described dandelion powder is: dandelion was pulverized to 50 mesh sieves.
A kind of alternative antibiotic pig green feed additive in the present invention, its preparation method is as follows: all raw materials mixed by formula rate, pulverized 60 mesh sieves.
A kind of alternative antibiotic pig green feed additive in the present invention, the addition in pig feed is 800 grams/ton.
Experimental example
360 of the health pig that subordinate plant of test selection twins (group) limited company average weight is 47.2kg, statistical analysis body weight difference is not remarkable.Be divided at random 6 groups, every group of 6 repetitions, 10 male and females half and half of each repetition, comprising 1 control group and 5 test group.Control group pig adopts basal diet to feed, and all the other 5 test group are added respectively the additive that embodiment 1-5 makes in basal diet, and live pig free choice feeding and drinking-water carry out immunity and expelling parasite routinely, observes and record the incidence of pig in experimental period every day.Totally 30 days experimental period, during off-test, weigh, and ramming material.Calculate each processed group daily gain, feedstuff-meat ratio, daily ingestion amount and the incidence of disease.
Result of the test shows that the feed interpolation that the present invention makes can significantly reduce the incidence of disease of pig, has strengthened the immunity of pig, can also increase the daily gain of live pig simultaneously and reduce feedstuff-meat ratio, and the growth and the raising efficiency of feed utilization that promote live pig are had very great help.
Products material of the present invention is green safety material, and can significantly reduce the incidence of disease of pig, strengthen the immunity of pig, promote the growth rate of pig, can be used as antibiotic substitute adds in pig feed, production disease resisting effect is good, effectively promotes the mixed feed of pig growth simultaneously, has good using value and wide market prospects.
Claims (7)
1. an alternative antibiotic pig green feed additive, is characterized in that being comprised of following raw materials by weight portion: fermentation of Chinese herbal medicine powder 10-15 part, gadol extract 1-3 part, Folium Artemisiae Argyi powder 4-8 part, radix glehniae powder 3-6 part, Eucommia Leaf Powder 2-6 part, caulis lonicerae powder 2-8 part, accumulated snow grass meal 3-8 part, balloonflower powder 2-6 part, dandelion powder 1-4 part;
The preparation method of described fermentation of Chinese herbal medicine powder is:
(1) by selected cattail pollen, rascal, the vine of multiflower knotweed, tamarind, the fruit of Chinese magnoliavine and mother chrysanthemum in mass ratio 2-3:1-4:2-5:1-3:2-4:2-5 co-grinding cross 30 mesh sieves, with 75% the ethanol spraying sterilizing 30-60 minute that evenly carries out disinfection, through 55 ℃ of oven dry, obtain mixing Chinese herbal medicine powder;
(2) mixing Chinese herbal medicine powder and composite fermentation liquid are mixed by solid-to-liquid ratio 100:1-3, must contain bacterium Chinese herbal medicine powder; Described composite fermentation liquid is in every 100ml composite fermentation liquid, to contain bacillus licheniformis 25ml, bacillus subtilis 35ml, bifidobacterium breve 18 ml, Rhodopseudomonas palustris 22 ml, and wherein the viable count of bacillus licheniformis is 2.4 × 10
8cfu/ml, the viable count of bacillus subtilis is 1.8 × 10
9the viable count of cfu/ml, bifidobacterium breve is 2.1 × 10
8the viable count of cfu/ml, Rhodopseudomonas palustris is 1.6 × 10
7cfu/ml;
(3) will, containing bacterium Chinese herbal medicine powder and fermentation medium 48:2-3 in mass ratio, mix, then add sterilized water by solid-to-liquid ratio 1:1, fermentation 48-72h, obtains fermentation of Chinese herbal medicine powder, by fermentation of Chinese herbal medicine powder drying and crushing, crosses 60 mesh sieves; Described fermentation medium is by weight being comprised of 0.1 part of 8 parts of glucose, 5 parts of peptones, 5 parts of beef extracts, 0.3 part, sodium chloride, 0.2 part, magnesium sulfate, manganese sulfate.
2. a kind of alternative antibiotic pig green feed additive according to claim 1, is characterized in that being comprised of following raw materials by weight portion: 13 parts, fermentation of Chinese herbal medicine powder, 2 parts of gadol extracts, 6 parts of Folium Artemisiae Argyi powders, 5 parts, radix glehniae powder, 4 parts of Eucommia Leaf Powders, 5 parts, caulis lonicerae powder, 6 parts of accumulated snow grass meals, 4 parts of balloonflower powders, 3 parts, dandelion powder;
The preparation method of described fermentation of Chinese herbal medicine powder is:
(1) by selected cattail pollen, rascal, the vine of multiflower knotweed, tamarind, the fruit of Chinese magnoliavine and mother chrysanthemum in mass ratio 3:2:4:2:3:4 co-grinding cross 30 mesh sieves, with 75% the ethanol spraying sterilizing 45 minutes that evenly carries out disinfection, through 55 ℃ of oven dry, obtain mixing Chinese herbal medicine powder;
(2) mixing Chinese herbal medicine powder and composite fermentation liquid are mixed by solid-to-liquid ratio 100:2, must contain bacterium Chinese herbal medicine powder; Described composite fermentation liquid is in every 100ml composite fermentation liquid, to contain bacillus licheniformis 25ml, bacillus subtilis 35ml, bifidobacterium breve 18 ml, Rhodopseudomonas palustris 22 ml, and wherein the viable count of bacillus licheniformis is 2.4 × 10
8cfu/ml, the viable count of bacillus subtilis is 1.8 × 10
9the viable count of cfu/ml, bifidobacterium breve is 2.1 × 10
8the viable count of cfu/ml, Rhodopseudomonas palustris is 1.6 × 10
7cfu/ml;
(3) will, containing bacterium Chinese herbal medicine powder and fermentation medium 48:3 in mass ratio, mix, then add sterilized water by solid-to-liquid ratio 1:1, fermentation 62h, obtains fermentation of Chinese herbal medicine powder, by fermentation of Chinese herbal medicine powder drying and crushing, crosses 60 mesh sieves; Described fermentation medium is by weight being comprised of 0.1 part of 8 parts of glucose, 5 parts of peptones, 5 parts of beef extracts, 0.3 part, sodium chloride, 0.2 part, magnesium sulfate, manganese sulfate.
3. according to a kind of alternative antibiotic pig green feed additive described in claim 1-2, it is characterized in that described gadol extract, its preparation method is: rhodiola root was pulverized to 40 mesh sieves, by rhodiola root powder and complex enzyme in mass ratio 100:1-3 mix, then add distilled water by solid-to-liquid ratio 1:3, heating maintains 48 ℃ by water temperature, carry out enzymolysis, enzymolysis time 1-2h, in enzymolysis process, every 10 minutes stir 1 time, after enzymolysis is complete by dry rhodiola root dried bean noodles; The ethanol of rhodiola root powder after enzymolysis and 60-75% is mixed by solid-to-liquid ratio 1:5-8, and ultrasonic extraction 30-40 minute, filters, and filtrate drying and crushing is crossed to 60 mesh sieves, obtains gadol extract; Described complex enzyme is cellulase and pectase 3:2 composition in mass ratio.
4. a kind of alternative antibiotic pig green feed additive according to claim 3, it is characterized in that described gadol extract, its preparation method is: rhodiola root was pulverized to 40 mesh sieves, by rhodiola root powder and complex enzyme in mass ratio 100:2 mix, then add distilled water by solid-to-liquid ratio 1:3, heating maintains 48 ℃ by water temperature, carry out enzymolysis, enzymolysis time 1.5h, in enzymolysis process, every 10 minutes stir 1 time, after enzymolysis is complete by dry rhodiola root dried bean noodles; The ethanol of the rhodiola root powder after enzymolysis and 70% is mixed by solid-to-liquid ratio 1:7, and ultrasonic extraction 35 minutes, filters, and filtrate drying and crushing is crossed to 60 mesh sieves, obtains gadol extract; Described complex enzyme is cellulase and pectase 3:2 composition in mass ratio.
5. according to a kind of alternative antibiotic pig green feed additive described in claim 1 or 2, it is characterized in that its preparation method is:
Step 1: Folium Artemisiae Argyi powder, radix glehniae powder, Eucommia Leaf Powder, caulis lonicerae powder, accumulated snow grass meal, balloonflower powder and dandelion powder are mixed by formula rate, by solid-to-liquid ratio, 1:4-8 adds distilled water, the 1-2h that refluxes under 50-60 ℃ of condition, refluxes and finishes rear drying and crushing and cross 60 mesh sieves, stand-by;
Step 2: step 1 is made to thing and fermentation of Chinese herbal medicine powder and gadol extract and mix and obtain additive of the present invention.
6. according to a kind of alternative antibiotic pig green feed additive described in claim 1 or 2, it is characterized in that its addition in pig feed is 600-1000 gram/ton.
7. a kind of alternative antibiotic pig green feed additive according to claim 6, is characterized in that its addition in pig feed is 800 grams/ton.
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| CN108541809A (en) * | 2018-04-11 | 2018-09-18 | 浙江博信药业股份有限公司 | A kind of livestock and poultry Chinese herb feed additive and its preparation method and application |
| CN109221622A (en) * | 2018-11-26 | 2019-01-18 | 大兴安岭北天原生物科技有限责任公司 | Subtract anti-feed and preparation method thereof containing Radix Astragali or Radix Astragali stem, leaf, flower |
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Effective date of registration: 20160111 Address after: 443005 No. 28 development avenue, Yichang Development Zone, Hubei Patentee after: Yichang twin Feed Co., Ltd. Address before: 330096 Nanchang high tech Zone, torch street, No. 999, twins group, Jiangxi Patentee before: Twins (Group) Co., Ltd. |