CN105360613A - Novel green feed additive capable of replacing antibiotics - Google Patents

Novel green feed additive capable of replacing antibiotics Download PDF

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Publication number
CN105360613A
CN105360613A CN201510832872.9A CN201510832872A CN105360613A CN 105360613 A CN105360613 A CN 105360613A CN 201510832872 A CN201510832872 A CN 201510832872A CN 105360613 A CN105360613 A CN 105360613A
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powder
leaf
mesh sieves
parts
bacterium powder
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赵艳平
李世传
吴志青
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Yuxi Twins Feed Co Ltd
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Yuxi Twins Feed Co Ltd
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Abstract

The invention discloses a novel green feed additive capable of replacing antibiotics, and belongs to the field of feed additives. The feed additive is composed of a micro-ecological preparation, a platycladus orientalis leaf extract, a mangnolia officinalis powder, a folium cortex eucommiae powder, a liquorice powder, a mint powder, a Chinese torreya seed powder, vitamin A, biotin, nicotinamide and a carrier, wherein the carrier is composed of defatted rice bran and peanut shells according to the mass ratio of (3-5) to 1. The feed additive is capable of replacing the antibiotics to be applied in live pig feeds, not only has small toxic and side effects, cannot produce drug residues and has green, safety and other advantages, but also has great help in improving the growth immunity and promoting growth, can effectively improve the breeding benefits, and has sufficiently broad application prospects.

Description

A kind of alternative antibiotic novel green feed addictive
Technical field
The present invention relates to a kind of feed addictive, especially relate to a kind of alternative antibiotic novel green feed addictive, belong to feed additive field.
Technical background
Feed safety is the significant problem of world today's extensive concern, begin from the forties, aquaculture starts progressively to widely use antibiotic, for animal husbandry fast development serves huge impetus, but along with long-term, the antibiotic use of high dose, also create following problem.Long-Time Service antibiotic makes animal body produce a large amount of antibody-resistant bacterium, increases treatment difficulty, even cannot treat, and causes mortality of animals high, and cultivation loss is comparatively large, is more unfavorable for the development that animal husbandry is healthy, optimum; In animal body, have one period of residence time after antibiotic metabolism, if now eaten by people, then can affect body immunity and premunition, long-term taking can cause immune function of human body disorderly, buries huge hidden danger in Blight control; Aquaculture still has a large amount of casual household and on a small scale intensive manufacture person, and supervision difficulty is large, and supervision exists leak and blank, even if strengthen regulatory efforts, fundamentally can not solve the residual problem such as higher of abuse of antibiotics, medicine.For this reason, European Union, the U.S., Japan and other countries release one after another a series of compulsory measure, limit or forbid adding antibiotic in feed.Along with the fast development of China's animal husbandry, antibiotic problem progressively causes extensive attention, China starts progressively antibiotic interpolation kind and use amount in limiting feed already, " nonreactive " feed has become the main trend of following animal husbandry development, for this reason, the research and development of Substitutes For Antibiotic have become the focus of animal husbandry benign development.
So the application in feed such as develop a kind of green, safe additive carrys out substitute antibiotics, nontoxic, noresidue, to have no drug resistance, meet the needs of social development, simultaneously to the sustainable health development important in inhibiting of animal husbandry.
Summary of the invention
The object of the present invention is to provide and to be made up of natural component, alternative antibiotic novel green feed addictive, this additive not only toxic and side effect little, can not medicament residue be produced, possess the advantages such as green safety, and the immunity of raising growth, the growth tool of growth promoting effects are very helpful, effectively can improve culture benefit.
For achieving the above object, the present invention adopts following technical scheme:
A kind of alternative antibiotic novel green feed addictive, be made up of following raw materials by weight portion: probiotics 8-12 part, Cacumen Platycladi extract 1-4 part, bark of official magnolia powder 3-5 part, Eucommia Leaf Powder 2-6 part, licorice powder 2-5 part, Herba Menthae powder 1-3 part, Chinese torreyanut powder 1-3 part, vitamin A 0.02-0.06 part, biotin 0.04-0.08 part, niacinamide 0.02-0.06 part, all the other are carrier, carrier by defatted rice bran and peanut shell in mass ratio 3-5:1 form, defatted rice bran and peanut shell pulverized 40 mesh sieves in advance;
The preparation method of described probiotics is:
(1) zymogenic preparation: by the 2-4:1-3:2-4 mixing in mass ratio of bacillus lentus bacterium powder, lactobacillus cellobiosas bacterium powder, lactoenterococcus bacterium powder, obtain composite bacterium powder, the viable count wherein in bacillus lentus bacterium powder is 1.5 × 10 8viable count in cfu/g, lactobacillus cellobiosas bacterium powder is 2.0 × 10 7viable count in cfu/g, lactoenterococcus bacterium powder is 1.0 × 10 9cfu/g; In composite bacterium powder, add sterilized water, the addition of sterilized water is 10% of compound bacteria quality, then is placed in incubator and cultivates 24h, and cultivation temperature is 37-42 DEG C, to cultivate after terminating to obtain zymophyte, stand-by;
(2) preparation of fermentation substrate: by weight alfalfa meal 5-10 part, sweet potato dregs 3-5 part, beef extract 1-3 part, peptone 1-3 part, ferrous fumarate 0.01-0.04 part, the mixing of cobalt chloride 0.02-0.04 part are pulverized, cross 20 mesh sieves, sterilizing 15min under 121 DEG C of conditions, obtain fermentation substrate, stand-by;
(3) ferment: by fermentation substrate obtained in zymophyte obtained in (1) and (2) 2-4:85-93 mixing in mass ratio, add sterilized water again, the addition of sterilized water is 15% of fermentation substrate quality, stir, at 37-42 DEG C of condition bottom fermentation 48-60h, after fermentation ends, carry out vacuum drying, vacuum drying temperature is 45 DEG C, then pulverizes 60 mesh sieves, obtains probiotics.
The preparation method of described Cacumen Platycladi extract is: freezing 12h under fresh side uncle's leaf is put in-80 DEG C of conditions, shreds, then add the ethanol of 95% after freezing end, addition is 5 times of side uncle's leaf quality, soaks 8h, soaks after terminating and is dried by side uncle's leaf, pulverize 20 mesh sieves, obtain side uncle's leaf powder; By solid-to-liquid ratio 1:5-10 distilled water in the uncle's leaf powder of side, carry out ultrasonic extraction, extraction time 30-40min, Extracting temperature 75 DEG C, extract power 180-220W, extract after terminating, filter, get filtrate, filter vacuum be dry, pulverize 60 mesh sieves, obtained side uncle's leaf extract.
The preparation method of described bark of official magnolia powder is: bark of official magnolia drying and crushing is crossed 60 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae drying and crushing is crossed 60 mesh sieves;
The preparation method of described licorice powder is: Radix Glycyrrhizae drying and crushing is crossed 60 mesh sieves;
The preparation method of described Herba Menthae powder is: peppermint drying and crushing is crossed 60 mesh sieves;
The preparation method of described Chinese torreyanut powder is: Chinese torreyanut drying and crushing is crossed 60 mesh sieves;
A kind of alternative antibiotic novel green feed addictive described in the present invention, the addition in feedstuff for live pigs is 1500-2200g/t.
Beneficial effect
The present invention is composite by composition science such as probiotics, Cacumen Platycladi extract, bark of official magnolia powder, Eucommia Leaf Powder, licorice powder, Herba Menthae powders, thus obtains a kind of alternative antibiotic novel green feed addictive.This additive is made up of natural component, and toxic and side effect is little, can not produce medicament residue, possesses the advantages such as green safety.This additive is applied in feedstuff for live pigs, effectively can not only improve the immunity of live pig, reduces the incidence of disease of pig, can also improve the growth performance of pig simultaneously, increases daily gain, shortens growth marketing time, has valuable help to increase culture benefit.The formula components of product of the present invention is natural products, green safety simultaneously, meets social development, to the sustainable health development important in inhibiting of animal husbandry.
Specific embodiment
Below by way of specific embodiment, the present invention is set forth further.
Embodiment 1
A kind of alternative antibiotic novel green feed addictive, be made up of following raw materials by weight portion: probiotics 8 parts, Cacumen Platycladi extract 1 part, 3 parts, bark of official magnolia powder, Eucommia Leaf Powder 2 parts, licorice powder 2 parts, Herba Menthae powder 1 part, 1 part, Chinese torreyanut powder, vitamin A 0.02 part, biotin 0.04 part, niacinamide 0.02 part, all the other are carrier, carrier by defatted rice bran and peanut shell in mass ratio 3:1 form, defatted rice bran and peanut shell pulverized 40 mesh sieves in advance;
The preparation method of described probiotics is:
(1) zymogenic preparation: by the 2:1:2 mixing in mass ratio of bacillus lentus bacterium powder, lactobacillus cellobiosas bacterium powder, lactoenterococcus bacterium powder, obtain composite bacterium powder, the viable count wherein in bacillus lentus bacterium powder is 1.5 × 10 8viable count in cfu/g, lactobacillus cellobiosas bacterium powder is 2.0 × 10 7viable count in cfu/g, lactoenterococcus bacterium powder is 1.0 × 10 9cfu/g; In composite bacterium powder, add sterilized water, the addition of sterilized water is 10% of compound bacteria quality, then is placed in incubator and cultivates 24h, and cultivation temperature is 37 DEG C, to cultivate after terminating to obtain zymophyte, stand-by;
(2) preparation of fermentation substrate: by weight alfalfa meal 5 parts, sweet potato dregs 3 parts, beef extract 1 part, peptone 1 part, ferrous fumarate 0.01 part, cobalt chloride 0.02 part mixing are pulverized, cross 20 mesh sieves, under 121 DEG C of conditions, sterilizing 15min, obtains fermentation substrate, stand-by;
(3) ferment: by fermentation substrate obtained in zymophyte obtained in (1) and (2) 2:85 mixing in mass ratio, add sterilized water again, the addition of sterilized water is 15% of fermentation substrate quality, stir, at 37 DEG C of condition bottom fermentation 48h, after fermentation ends, carry out vacuum drying, vacuum drying temperature is 45 DEG C, then pulverizes 60 mesh sieves, obtains probiotics.
The preparation method of described Cacumen Platycladi extract is: freezing 12h under fresh side uncle's leaf is put in-80 DEG C of conditions, shreds, then add the ethanol of 95% after freezing end, addition is 5 times of side uncle's leaf quality, soaks 8h, soaks after terminating and is dried by side uncle's leaf, pulverize 20 mesh sieves, obtain side uncle's leaf powder; By solid-to-liquid ratio 1:5 distilled water in the uncle's leaf powder of side, carry out ultrasonic extraction, extraction time 30min, Extracting temperature 75 DEG C, extract power 180W, extract after terminating, filter, get filtrate, filter vacuum be dry, pulverize 60 mesh sieves, obtain side uncle's leaf extract.
The preparation method of described bark of official magnolia powder is: bark of official magnolia drying and crushing is crossed 60 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae drying and crushing is crossed 60 mesh sieves;
The preparation method of described licorice powder is: Radix Glycyrrhizae drying and crushing is crossed 60 mesh sieves;
The preparation method of described Herba Menthae powder is: peppermint drying and crushing is crossed 60 mesh sieves;
The preparation method of described Chinese torreyanut powder is: Chinese torreyanut drying and crushing is crossed 60 mesh sieves;
A kind of alternative antibiotic novel green feed addictive described in the present invention, the addition in feedstuff for live pigs is 1500g/t.
Embodiment 2
A kind of alternative antibiotic novel green feed addictive, be made up of following raw materials by weight portion: probiotics 10 parts, Cacumen Platycladi extract 3 parts, 4 parts, bark of official magnolia powder, Eucommia Leaf Powder 4 parts, licorice powder 3 parts, Herba Menthae powder 2 parts, 2 parts, Chinese torreyanut powder, vitamin A 0.04 part, biotin 0.06 part, niacinamide 0.04 part, all the other are carrier, carrier by defatted rice bran and peanut shell in mass ratio 4:1 form, defatted rice bran and peanut shell pulverized 40 mesh sieves in advance;
The preparation method of described probiotics is:
(1) zymogenic preparation: by the 3:2:3 mixing in mass ratio of bacillus lentus bacterium powder, lactobacillus cellobiosas bacterium powder, lactoenterococcus bacterium powder, obtain composite bacterium powder, the viable count wherein in bacillus lentus bacterium powder is 1.5 × 10 8viable count in cfu/g, lactobacillus cellobiosas bacterium powder is 2.0 × 10 7viable count in cfu/g, lactoenterococcus bacterium powder is 1.0 × 10 9cfu/g; In composite bacterium powder, add sterilized water, the addition of sterilized water is 10% of compound bacteria quality, then is placed in incubator and cultivates 24h, and cultivation temperature is 39 DEG C, to cultivate after terminating to obtain zymophyte, stand-by;
(2) preparation of fermentation substrate: by weight alfalfa meal 8 parts, sweet potato dregs 4 parts, beef extract 2 parts, peptone 2 parts, ferrous fumarate 0.03 part, cobalt chloride 0.03 part mixing are pulverized, cross 20 mesh sieves, under 121 DEG C of conditions, sterilizing 15min, obtains fermentation substrate, stand-by;
(3) ferment: by fermentation substrate obtained in zymophyte obtained in (1) and (2) 3:89 mixing in mass ratio, add sterilized water again, the addition of sterilized water is 15% of fermentation substrate quality, stir, at 39 DEG C of condition bottom fermentation 54h, after fermentation ends, carry out vacuum drying, vacuum drying temperature is 45 DEG C, then pulverizes 60 mesh sieves, obtains probiotics.
The preparation method of described Cacumen Platycladi extract is: freezing 12h under fresh side uncle's leaf is put in-80 DEG C of conditions, shreds, then add the ethanol of 95% after freezing end, addition is 5 times of side uncle's leaf quality, soaks 8h, soaks after terminating and is dried by side uncle's leaf, pulverize 20 mesh sieves, obtain side uncle's leaf powder; By solid-to-liquid ratio 1:8 distilled water in the uncle's leaf powder of side, carry out ultrasonic extraction, extraction time 35min, Extracting temperature 75 DEG C, extract power 200W, extract after terminating, filter, get filtrate, filter vacuum be dry, pulverize 60 mesh sieves, obtain side uncle's leaf extract.
The preparation method of described bark of official magnolia powder is: bark of official magnolia drying and crushing is crossed 60 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae drying and crushing is crossed 60 mesh sieves;
The preparation method of described licorice powder is: Radix Glycyrrhizae drying and crushing is crossed 60 mesh sieves;
The preparation method of described Herba Menthae powder is: peppermint drying and crushing is crossed 60 mesh sieves;
The preparation method of described Chinese torreyanut powder is: Chinese torreyanut drying and crushing is crossed 60 mesh sieves;
A kind of alternative antibiotic novel green feed addictive described in the present invention, the addition in feedstuff for live pigs is 1900g/t.
Embodiment 3
A kind of alternative antibiotic novel green feed addictive, be made up of following raw materials by weight portion: probiotics 12 parts, Cacumen Platycladi extract 4 parts, 5 parts, bark of official magnolia powder, Eucommia Leaf Powder 6 parts, licorice powder 5 parts, Herba Menthae powder 3 parts, 3 parts, Chinese torreyanut powder, vitamin A 0.06 part, biotin 0.08 part, niacinamide 0.06 part, all the other are carrier, carrier by defatted rice bran and peanut shell in mass ratio 5:1 form, defatted rice bran and peanut shell pulverized 40 mesh sieves in advance;
The preparation method of described probiotics is:
(1) zymogenic preparation: by the 4:3:4 mixing in mass ratio of bacillus lentus bacterium powder, lactobacillus cellobiosas bacterium powder, lactoenterococcus bacterium powder, obtain composite bacterium powder, the viable count wherein in bacillus lentus bacterium powder is 1.5 × 10 8viable count in cfu/g, lactobacillus cellobiosas bacterium powder is 2.0 × 10 7viable count in cfu/g, lactoenterococcus bacterium powder is 1.0 × 10 9cfu/g; In composite bacterium powder, add sterilized water, the addition of sterilized water is 10% of compound bacteria quality, then is placed in incubator and cultivates 24h, and cultivation temperature is 42 DEG C, to cultivate after terminating to obtain zymophyte, stand-by;
(2) preparation of fermentation substrate: by weight alfalfa meal 10 parts, sweet potato dregs 5 parts, beef extract 3 parts, peptone 3 parts, ferrous fumarate 0.04 part, cobalt chloride 0.04 part mixing are pulverized, cross 20 mesh sieves, under 121 DEG C of conditions, sterilizing 15min, obtains fermentation substrate, stand-by;
(3) ferment: by fermentation substrate obtained in zymophyte obtained in (1) and (2) 4:93 mixing in mass ratio, add sterilized water again, the addition of sterilized water is 15% of fermentation substrate quality, stir, at 42 DEG C of condition bottom fermentation 60h, after fermentation ends, carry out vacuum drying, vacuum drying temperature is 45 DEG C, then pulverizes 60 mesh sieves, obtains probiotics.
The preparation method of described Cacumen Platycladi extract is: freezing 12h under fresh side uncle's leaf is put in-80 DEG C of conditions, shreds, then add the ethanol of 95% after freezing end, addition is 5 times of side uncle's leaf quality, soaks 8h, soaks after terminating and is dried by side uncle's leaf, pulverize 20 mesh sieves, obtain side uncle's leaf powder; By solid-to-liquid ratio 1:10 distilled water in the uncle's leaf powder of side, carry out ultrasonic extraction, extraction time 40min, Extracting temperature 75 DEG C, extract power 220W, extract after terminating, filter, get filtrate, filter vacuum be dry, pulverize 60 mesh sieves, obtain side uncle's leaf extract.
The preparation method of described bark of official magnolia powder is: bark of official magnolia drying and crushing is crossed 60 mesh sieves;
The preparation method of described Eucommia Leaf Powder is: folium cortex eucommiae drying and crushing is crossed 60 mesh sieves;
The preparation method of described licorice powder is: Radix Glycyrrhizae drying and crushing is crossed 60 mesh sieves;
The preparation method of described Herba Menthae powder is: peppermint drying and crushing is crossed 60 mesh sieves;
The preparation method of described Chinese torreyanut powder is: Chinese torreyanut drying and crushing is crossed 60 mesh sieves;
A kind of alternative antibiotic novel green feed addictive described in the present invention, the addition in feedstuff for live pigs is 2200g/t.
Embodiment 4
A kind of alternative antibiotic novel green feed addictive, be made up of following raw materials by weight portion: probiotics 3 parts, Cacumen Platycladi extract 1 part, 1 part, bark of official magnolia powder, Eucommia Leaf Powder 1 part, licorice powder 6 parts, Herba Menthae powder 6 parts, 1 part, Chinese torreyanut powder, vitamin A 0.01 part, biotin 0.02 part, niacinamide 0.01 part, all the other are carrier, carrier by defatted rice bran and peanut shell in mass ratio 1:1 form, defatted rice bran and peanut shell pulverized 40 mesh sieves in advance;
All the other are with embodiment 2.
Experimental example.
Subordinate plant of test selection Yuxi Twins Feed Co., Ltd. average weight is the health pig 300 of 45.6kg, and statistical analysis weight differences is not remarkable.Be divided into 5 groups at random, often organize 6 repetitions, each repetition 10 male and females half and half, comprising 1 control group and 4 test group.Control group live pig adopts basal diet to feed, and all the other 4 test group add the obtained additive of embodiment 1-4 respectively in basal diet, and live pig free choice feeding and drinking-water, carry out immunity and expelling parasite routinely, and in experimental period, every day observes and records the incidence of pig.Totally 30 days experimental period.Weigh during off-test, and ramming material.Calculate each processed group daily gain, feedstuff-meat ratio and the incidence of disease.
Project Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Control group
All the time (kg) 45.1 44.8 46.2 45.8 46.1
Daily gain (g/d head) 855 892 871 807 761
Feedstuff-meat ratio 2.27 2.17 2.22 2.49 2.53
The incidence of disease 2.20% 1.60% 1.90% 14.20% 33.40%
Result of the test shows: in live pig daily ration, add product of the present invention, significantly can reduce the incidence of disease of live pig, illustrates that product of the present invention can strengthen the immunity of live pig; The present invention simultaneously has very great help to the daily gain and reduction feedstuff-meat ratio tool that increase live pig, illustrates that product of the present invention can improve the growth performance of growth, is conducive to improving pig-breeding benefit.
Products material of the present invention is green safety material, and in feed, add the incidence of disease that significantly can reduce pig, promotes the growth performance of pig, can add in pig feed as antibiotic substitute, have good using value and wide market prospects.

Claims (6)

1. an alternative antibiotic novel green feed addictive, it is characterized in that being made up of following raw materials by weight portion: probiotics 8-12 part, Cacumen Platycladi extract 1-4 part, bark of official magnolia powder 3-5 part, Eucommia Leaf Powder 2-6 part, licorice powder 2-5 part, Herba Menthae powder 1-3 part, Chinese torreyanut powder 1-3 part, vitamin A 0.02-0.06 part, biotin 0.04-0.08 part, niacinamide 0.02-0.06 part, all the other are carrier, carrier by defatted rice bran and peanut shell in mass ratio 3-5:1 form, defatted rice bran and peanut shell pulverized 40 mesh sieves in advance;
The preparation method of described probiotics is:
(1) zymogenic preparation: by the 2-4:1-3:2-4 mixing in mass ratio of bacillus lentus bacterium powder, lactobacillus cellobiosas bacterium powder, lactoenterococcus bacterium powder, obtain composite bacterium powder, the viable count wherein in bacillus lentus bacterium powder is 1.5 × 10 8viable count in cfu/g, lactobacillus cellobiosas bacterium powder is 2.0 × 10 7viable count in cfu/g, lactoenterococcus bacterium powder is 1.0 × 10 9cfu/g; In composite bacterium powder, add sterilized water, the addition of sterilized water is 10% of compound bacteria quality, then is placed in incubator and cultivates 24h, and cultivation temperature is 37-42 DEG C, to cultivate after terminating to obtain zymophyte, stand-by;
(2) preparation of fermentation substrate: by weight alfalfa meal 5-10 part, sweet potato dregs 3-5 part, beef extract 1-3 part, peptone 1-3 part, ferrous fumarate 0.01-0.04 part, the mixing of cobalt chloride 0.02-0.04 part are pulverized, cross 20 mesh sieves, sterilizing 15min under 121 DEG C of conditions, obtain fermentation substrate, stand-by;
(3) ferment: by fermentation substrate obtained in zymophyte obtained in (1) and (2) 2-4:85-93 mixing in mass ratio, add sterilized water again, the addition of sterilized water is 15% of fermentation substrate quality, stir, at 37-42 DEG C of condition bottom fermentation 48-60h, after fermentation ends, carry out vacuum drying, vacuum drying temperature is 45 DEG C, then pulverizes 60 mesh sieves, obtains probiotics.
2. a kind of alternative antibiotic novel green feed addictive according to claim 1, it is characterized in that being made up of following raw materials by weight portion: probiotics 10 parts, Cacumen Platycladi extract 3 parts, 4 parts, bark of official magnolia powder, Eucommia Leaf Powder 4 parts, licorice powder 3 parts, Herba Menthae powder 2 parts, 2 parts, Chinese torreyanut powder, vitamin A 0.04 part, biotin 0.06 part, niacinamide 0.04 part, all the other are carrier, carrier by defatted rice bran and peanut shell in mass ratio 4:1 form, defatted rice bran and peanut shell pulverized 40 mesh sieves in advance;
The preparation method of described probiotics is:
(1) zymogenic preparation: by the 3:2:3 mixing in mass ratio of bacillus lentus bacterium powder, lactobacillus cellobiosas bacterium powder, lactoenterococcus bacterium powder, obtain composite bacterium powder, the viable count wherein in bacillus lentus bacterium powder is 1.5 × 10 8viable count in cfu/g, lactobacillus cellobiosas bacterium powder is 2.0 × 10 7viable count in cfu/g, lactoenterococcus bacterium powder is 1.0 × 10 9cfu/g; In composite bacterium powder, add sterilized water, the addition of sterilized water is 10% of compound bacteria quality, then is placed in incubator and cultivates 24h, and cultivation temperature is 39 DEG C, to cultivate after terminating to obtain zymophyte, stand-by;
(2) preparation of fermentation substrate: by weight alfalfa meal 8 parts, sweet potato dregs 4 parts, beef extract 2 parts, peptone 2 parts, ferrous fumarate 0.03 part, cobalt chloride 0.03 part mixing are pulverized, cross 20 mesh sieves, under 121 DEG C of conditions, sterilizing 15min, obtains fermentation substrate, stand-by;
(3) ferment: by fermentation substrate obtained in zymophyte obtained in (1) and (2) 3:89 mixing in mass ratio, add sterilized water again, the addition of sterilized water is 15% of fermentation substrate quality, stir, at 39 DEG C of condition bottom fermentation 54h, after fermentation ends, carry out vacuum drying, vacuum drying temperature is 45 DEG C, then pulverizes 60 mesh sieves, obtains probiotics.
3. a kind of alternative antibiotic novel green feed addictive according to claim 1 or 2, it is characterized in that the preparation method of described Cacumen Platycladi extract is: freezing 12h under fresh side uncle's leaf is put in-80 DEG C of conditions, shred after freezing end, add the ethanol of 95% again, addition is 5 times of side uncle's leaf quality, soaks 8h, soaks after terminating and is dried by side uncle's leaf, pulverize 20 mesh sieves, obtain side uncle's leaf powder; By solid-to-liquid ratio 1:5-10 distilled water in the uncle's leaf powder of side, carry out ultrasonic extraction, extraction time 30-40min, Extracting temperature 75 DEG C, extract power 180-220W, extract after terminating, filter, get filtrate, filter vacuum be dry, pulverize 60 mesh sieves, obtained side uncle's leaf extract.
4. a kind of alternative antibiotic novel green feed addictive according to claim 3, it is characterized in that the preparation method of described Cacumen Platycladi extract is: freezing 12h under fresh side uncle's leaf is put in-80 DEG C of conditions, shred after freezing end, add the ethanol of 95% again, addition is 5 times of side uncle's leaf quality, soaks 8h, soaks after terminating and is dried by side uncle's leaf, pulverize 20 mesh sieves, obtain side uncle's leaf powder; By solid-to-liquid ratio 1:8 distilled water in the uncle's leaf powder of side, carry out ultrasonic extraction, extraction time 35min, Extracting temperature 75 DEG C, extract power 200W, extract after terminating, filter, get filtrate, filter vacuum be dry, pulverize 60 mesh sieves, obtain side uncle's leaf extract.
5. a kind of alternative antibiotic novel green feed addictive according to any one of claim 1-4, is characterized in that the addition in feedstuff for live pigs is 1500-2200g/t.
6. a kind of alternative antibiotic novel green feed addictive according to any one of claim 1-4, is characterized in that the addition in feedstuff for live pigs is 1900g/t.
CN201510832872.9A 2015-11-26 2015-11-26 Novel green feed additive capable of replacing antibiotics Pending CN105360613A (en)

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CN107691785A (en) * 2017-10-30 2018-02-16 广州傲农生物科技有限公司 A kind of feed addictive containing plant extracts and preparation method and application
CN108552423A (en) * 2018-03-31 2018-09-21 潍坊友容实业有限公司 Cattle feed additive/premixed material and preparation method thereof
CN108651720A (en) * 2018-03-31 2018-10-16 潍坊友容实业有限公司 A kind of feed containing cattle feed additive/premixed material and its application
CN115024387A (en) * 2022-06-23 2022-09-09 陕西新雨丹中药材生物科技有限公司 Chinese herbal medicine feed additive and preparation method thereof

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CN103947906A (en) * 2014-05-21 2014-07-30 双胞胎(集团)股份有限公司 Pig microecological preparation and preparation method thereof
CN104286540A (en) * 2014-10-09 2015-01-21 胡兰春 Biological feed additive with function of improving intestinal health of pigs

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* Cited by examiner, † Cited by third party
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CN106387433A (en) * 2016-10-10 2017-02-15 枞阳县义津畜禽综合养殖有限责任公司 Wild traditional Chinese medicine type live pig feed
CN107691785A (en) * 2017-10-30 2018-02-16 广州傲农生物科技有限公司 A kind of feed addictive containing plant extracts and preparation method and application
CN108552423A (en) * 2018-03-31 2018-09-21 潍坊友容实业有限公司 Cattle feed additive/premixed material and preparation method thereof
CN108651720A (en) * 2018-03-31 2018-10-16 潍坊友容实业有限公司 A kind of feed containing cattle feed additive/premixed material and its application
CN115024387A (en) * 2022-06-23 2022-09-09 陕西新雨丹中药材生物科技有限公司 Chinese herbal medicine feed additive and preparation method thereof

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