CN103621825A - Preparation method and application of fermented Chinese herbal medicine immune enhancer for yellow-head catfish meat - Google Patents
Preparation method and application of fermented Chinese herbal medicine immune enhancer for yellow-head catfish meat Download PDFInfo
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Abstract
The invention discloses a fermented Chinese herbal medicine immune enhancer for yellow-head catfish meat. The fermented Chinese herbal medicine immune enhancer has a plurality of effects of prevention, health-care, immunoregulation and the like, and is an ideal, green, environment-friendly, safe and novel feed additive for replacing an antibiotic medicine. The fermented Chinese herbal medicine immune enhancer is prepared from Chinese herbal compounds by technologies of ultrasonic assisted extraction and multi-strain two-step fermentation, has double advantages of Chinese herbal medicines and probiotics, can efficiently regulate intestinal health of the yellow-head catfish meat, can treat both symptoms and root causes, and can strengthen healthy energy and cultivate the vitality; microecological balance and the best immune state of an intestinal tract are kept; the digestibility of nutrient substances is improved; the body immunity of the yellow-head catfish meat is improved; the farming income is increased; the breeding risk is reduced; the fermented Chinese herbal medicine immune enhancer is used instead of antibiotics, is free of a bad effect on the yellow-head catfish meat, and safe to use, has a broad market prospect, and does not generate the problems such as drug residues and tolerance.
Description
Technical field
The present invention relates to the preparation method of fermented type Chinese medicine immunity enhancer for a kind of Pelteobagrus fulvidraco, belong to feed additive field.
Background technology
Aquatic products more and more occupy important share in people's diet, in order to meet the demand of ever-increasing aquatic products, solution becomes the Important Problems of aquaculture because the physiological function that aquaculture scale constantly expands and growth cycle reduction causes is disorderly, disease takes place frequently, and is also the key of aquatic feeds industry development.Under this background, antibiotics feed addictive is subject to heat and holds in both hands, and is widely used in feed industry, has promoted to a certain extent the development of culture fishery, but more serious of the problems such as the high residue that antibiotic brings, high pollution and the resistance to the action of a drug.Current social, food security Frequent Accidents, people also more focus on healthy diet in satisfied having enough to eat and wear, and the aquatic products of green health receive much concern.Chinese herbal feed additive meets the requirement of green aquatic products aquaculture development, has eliminated the safety worries of people to its aquatic products product of feeding out, should vigorously promote the use.But the research level of current Chinese herbal feed additive is lower, manufacture craft simple coarse, the technological element of a product is lower, and quality is stable not, and problem like that is seriously restricting applying of Chinese medicine fodder interpolation.Microorganism fermented tcm is to the reform of traditional Chinese medicine process technology and innovation, has various features and advantage.This technology is introduced to the research of Chinese herbal feed additive, can make its scientific and technological content significantly promote, effect is more remarkable, more stable quality, benefit is more obvious, can significantly improve immunity of organisms, strengthen the level of aquatic livestock resist the disease, for the various problems that solves aquatic livestock in current culture fishery, have important using value.Utilize microbial fermentation technology to process Chinese herbal medicine, can make the content of its main active substances polysaccharide significantly improve, this seems particularly important for the culture fishery of focusing on aquaculture cost.
Pelteobagrus fulvidraco (
pseudobagrus fulvidraco Richardson) claim again loud, high-pitched sound serratd edge, Huang Lading, in classification, belong to Silurformes (
silurfoymes) ，Catfish section (
bagridae), Pelteobagrus (
pseudobagrus), this genus has 5 kinds, is respectively Pelteobagrus fulvidraco, pseudobagrus vachelli (Pelteobagrus vachelli), pseudobagrus nitidus, pseudobagrus eupogon and Pelteobagrus intermedius, is mainly Pelteobagrus fulvidraco and pseudobagrus vachelli at present for what propagate artificially.Pelteobagrus fulvidraco all has distribution in rivers and lakes pond, China most areas, although individuality is less, because of the delicious delicacy of its meat younger sister, few thorn, nutritious, get consumer reception very much, a yellow fish not only has tonic effect, and has certain medical value, and its adult fish individuality is generally at 100-250g.Huang Shunyu also has huge market potential in countries such as Japan, Korea S, Southeast Asia, is the improved seeds of foreign exchange earning.At present, due to natural underproduce, the Huang Shunyu naturally fishing for far can not meet human lives's needs, propagates artificially and becomes the topmost approach that Pelteobagrus fulvidraco is met the need of market.But in Culture of Pelteobagrus Fulvidraco process, also exist antibiotic and use the drawbacks such as excessive, cultivation has a big risk, become the bottleneck factor of restriction Pelteobagrus fulvidraco aquaculture sustainable development.
Summary of the invention
The object of the invention is to according to above shortcomings in prior art, antibacterial, antiviral, the left and right of improving immunity of organisms and improving growth of animal performance of considering that Chinese herbal medicine has, can reduce a large amount of aquacultures that cause of using of antibiotic pollutes, and raw material sources are wide, cheap, be a kind of green, environmental protection, efficient feed addictive, develop a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
A Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, is characterized in that compound Chinese herb is comprised of the Chinese herbal medicine of following mass ratio: old 12～18 parts of bacterium, 8～12 parts of golden cypresses, 8～12 parts of matrimony vines, 6～10 parts of honeysuckles, 3～5 parts of glossy ganodermas, 6～10 parts of milk thistles, 6～8 parts of radix bupleuri, 3～5 parts of the coptiss, 6～10 parts of rhizoma alismatis, 6～10 parts of the roots of kudzu vine, 6～10 parts of purslanes, 5～7 parts of the fleece-flowers root, 3～5 parts of SEMEN ASTRAGALI COMPLANATIs.
The invention provides a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, selected leavening is by bacillus subtilis (Chinese agriculture microorganism fungus kind preservation administrative center, bacterium numbering ACCC10619), S. cervisiae (Angel Yeast), lactobacillus bulgaricus (Chinese agriculture microorganism fungus kind preservation administrative center, bacterium numbering ACCC10638) and bifidobacterium bifidum (ATCC standard bacterial classification, bacterium numbering ATCC29521) composite ferment forming, bacillus subtilis wherein, lactobacillus bulgaricus, the inoculum concentration of bifidobacterium bifidum is 2～4%, S. cervisiae inoculum concentration is 5～8%.
The invention provides the preparation method of fermented type Chinese medicine immunity enhancer for a kind of Pelteobagrus fulvidraco, specifically comprise the following steps:
(1) preparation of compound Chinese herb: accurately weigh Chinese herbal medicine according to above-mentioned formula, be crushed to 40～60 orders, mix, make compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: to the water that adds 5～10 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(3) zymotic fluid preparation: add 3.0～5.0% soluble starches, 2.0～3.0% beancake powders, 0.3～0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(4) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 2～4%, 5～8%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 12～24h;
(5) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2～4%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 12～24h;
(6) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.2～2 volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(7) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
The invention provides a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, have prevention, health care, treatment and regulate the multiple efficacies such as immunity, is ideal, green, environmental protection, safety, the novel fodder additive of substitute antibiotics medicine.This invention fermentation of Chinese herbal medicine feed addictive one is efficiently to nurse one's health Pelteobagrus fulvidraco intestinal health, treats both principal and secondary aspect of disease, and reinvigoration, keeps intestinal microecology balance and best immune state, improves the digestibility of nutriment, improves animal body immunity; The 2nd, can suppress and stop the generation of the harmful bacterias such as cause of disease coliform, clostridium, salmonella, increase beneficial bacteria of intestinal tract, maintain healthy gut flora; The 3rd, strengthen immunization, by stimulating immunity in enteron aisle, be cell, increase the formation of local antibody, increase macrophage activity; The 4th, fermentation of Chinese herbal medicine feed addictive enters in intestines and resists harmful bacteria, reduces stench and harmful components and forms, and makes the ight soil of discharging eliminate stench, in protection breeding environment, improves Pelteobagrus fulvidraco meat quality.
The invention provides a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, described Pelteobagrus fulvidraco is 0.1～0.5 with the mass ratio that fermented type Chinese medicine immunity enhancer product is used in aquatic feeds complete diet pellet: or described feed addictive premix, concentrate feed in complete diet pellet required ratio add and use.
The invention provides a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, it is advantageous that: the one, in fermentation of Chinese herbal medicine feed addictive preparation process, adopted the production technology of " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+many bacterial classifications two-step fermentation ", to compare action effect more remarkable with existing Chinese herbal feed additive product; The 2nd, fermentation of Chinese herbal medicine feed addictive has the two-fold advantage of Chinese herbal medicine and probio, can efficiently nurse one's health Pelteobagrus fulvidraco intestinal health, treat both principal and secondary aspect of disease, reinvigoration, keep intestinal microecology balance and best immune state, improve the digestibility of nutriment, improve animal body immunity, improve cultivation income, reduce cultivation risk; The 3rd, the alternative antibiotic of this product is used, and Pelteobagrus fulvidraco is had no adverse effects, and uses safety, does not have the problems such as medicament residue and drug resistance, has wide market prospects.
The specific embodiment
embodiment 1:
A Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: according to following formula, accurately weigh Chinese herbal medicine: old 12 parts of bacterium, 12 parts of golden cypresses, 8 parts of matrimony vines, 10 parts of honeysuckles, 3 parts of glossy ganodermas, 10 parts of milk thistles, 6 parts of radix bupleuri, 5 parts of the coptiss, 6 parts of rhizoma alismatis, 10 parts of the roots of kudzu vine, 6 parts of purslanes, 7 parts of the fleece-flowers root, 3 parts of SEMEN ASTRAGALI COMPLANATIs;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 5 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(4) zymotic fluid preparation: add 3.0% soluble starch, 3.0% beancake powder, 0.3% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 2%, 8%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 12h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is respectively 2%, 4%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 24h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.2 times of volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
embodiment 2:
A Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: according to following formula, accurately weigh Chinese herbal medicine: old 18 parts of bacterium, 8 parts of golden cypresses, 12 parts of matrimony vines, 6 parts of honeysuckles, 5 parts of glossy ganodermas, 6 parts of milk thistles, 8 parts of radix bupleuri, 3 parts of the coptiss, 10 parts of rhizoma alismatis, 6 parts of the roots of kudzu vine, 10 parts of purslanes, 5 parts of the fleece-flowers root, 5 parts of SEMEN ASTRAGALI COMPLANATIs;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 10 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(4) zymotic fluid preparation: add 5.0% soluble starch, 2.0% beancake powder, 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 4%, 5%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 24h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is respectively 4%, 2%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 12h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 2 times of volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
embodiment 3:
A Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: according to following formula, accurately weigh Chinese herbal medicine: old 15 parts of bacterium, 10 parts of golden cypresses, 10 parts of matrimony vines, 8 parts of honeysuckles, 4 parts of glossy ganodermas, 8 parts of milk thistles, 7 parts of radix bupleuri, 4 parts of the coptiss, 8 parts of rhizoma alismatis, 8 parts of the roots of kudzu vine, 8 parts of purslanes, 6 parts of the fleece-flowers root, 4 parts of SEMEN ASTRAGALI COMPLANATIs;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 7.5 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(4) zymotic fluid preparation: add 4.0% soluble starch, 2.5% beancake powder, 0.4% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 3%, 6.5%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 18h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2～4%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 18h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.6 times of volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
Above embodiment is only for technical scheme of the present invention is described, but not is limited; Although with reference to previous embodiment, to being had been described in detail by invention, for the person of ordinary skill of the art, the technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And to these modifications or replacement, do not make the spirit and scope of essence disengaging the present invention technical scheme required for protection of appropriate technical solution.
Claims (2)
1. a Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, is characterized in that being made by " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+many bacterial classifications two-step fermentation " technique by compound Chinese herb.
2. compound Chinese herb according to claim 1, is characterized in that being comprised of the Chinese herbal medicine of following mass ratio: old 12～18 parts of bacterium, 8～12 parts of golden cypresses, 8～12 parts of matrimony vines, 6～10 parts of honeysuckles, 3～5 parts of glossy ganodermas, 6～10 parts of milk thistles, 6～8 parts of radix bupleuri, 3～5 parts of the coptiss, 6～10 parts of rhizoma alismatis, 6～10 parts of the roots of kudzu vine, 6～10 parts of purslanes, 5～7 parts of the fleece-flowers root, 3～5 parts of SEMEN ASTRAGALI COMPLANATIs.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222675A (en) * | 2014-09-30 | 2014-12-24 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of Chinese herbal immunostimulant for flatfish |
| CN104543372A (en) * | 2015-01-18 | 2015-04-29 | 新疆瑞源丰贸易有限公司 | Sunflower disk composite feed and preparation method thereof |
| CN104957437A (en) * | 2015-07-29 | 2015-10-07 | 青岛海之星生物科技有限公司 | Compound feed for Pelteobagrus fulvidraco and preparation method of compound feed |
| CN105265376A (en) * | 2015-09-15 | 2016-01-27 | 广东海洋大学 | Compound Chinese herbal medicine preparation capable of promoting multi-microecological preparation proliferation |
| CN105660470A (en) * | 2016-01-18 | 2016-06-15 | 广德县清元水产养殖专业合作社 | Method for ecological breeding of pelteobagrus fulvidraco |
| CN105660471A (en) * | 2016-01-18 | 2016-06-15 | 广德县清元水产养殖专业合作社 | Method for small-area breeding of pelteobagrus fulvidraco |
| CN106615996A (en) * | 2016-09-18 | 2017-05-10 | 广西大学 | Traditional Chinese medicine feed additive capable of improving disease resistance of grass carp and preparation method of traditional Chinese medicine feed additive |
| CN106721663A (en) * | 2016-12-28 | 2017-05-31 | 青岛嘉瑞生物技术有限公司 | A kind of method that bioanalysis prepares cobio expanding compound feed using glasswort |
| CN106819655A (en) * | 2017-02-17 | 2017-06-13 | 浙江万里学院 | A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield and its preparation application method |
| CN108606165A (en) * | 2018-04-19 | 2018-10-02 | 广东联鲲集团有限公司 | It is a kind of to improve the additive and its application that Pelteobagrus fulvidraco enteron aisle hardens |
| CN108782955A (en) * | 2018-07-12 | 2018-11-13 | 漳州傲农牧业科技有限公司 | A kind of feed and preparation method thereof improving Pelteobagrus fulvidraco body colour and meat |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222675A (en) * | 2014-09-30 | 2014-12-24 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of Chinese herbal immunostimulant for flatfish |
| CN104543372A (en) * | 2015-01-18 | 2015-04-29 | 新疆瑞源丰贸易有限公司 | Sunflower disk composite feed and preparation method thereof |
| CN104957437A (en) * | 2015-07-29 | 2015-10-07 | 青岛海之星生物科技有限公司 | Compound feed for Pelteobagrus fulvidraco and preparation method of compound feed |
| CN105265376B (en) * | 2015-09-15 | 2018-10-19 | 广东海洋大学 | A kind of Chinese herbal formula promoting a variety of probiotics in-vitro multiplications |
| CN105265376A (en) * | 2015-09-15 | 2016-01-27 | 广东海洋大学 | Compound Chinese herbal medicine preparation capable of promoting multi-microecological preparation proliferation |
| CN105660470A (en) * | 2016-01-18 | 2016-06-15 | 广德县清元水产养殖专业合作社 | Method for ecological breeding of pelteobagrus fulvidraco |
| CN105660471A (en) * | 2016-01-18 | 2016-06-15 | 广德县清元水产养殖专业合作社 | Method for small-area breeding of pelteobagrus fulvidraco |
| CN106615996A (en) * | 2016-09-18 | 2017-05-10 | 广西大学 | Traditional Chinese medicine feed additive capable of improving disease resistance of grass carp and preparation method of traditional Chinese medicine feed additive |
| CN106721663A (en) * | 2016-12-28 | 2017-05-31 | 青岛嘉瑞生物技术有限公司 | A kind of method that bioanalysis prepares cobio expanding compound feed using glasswort |
| CN106819655A (en) * | 2017-02-17 | 2017-06-13 | 浙江万里学院 | A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield and its preparation application method |
| CN108606165A (en) * | 2018-04-19 | 2018-10-02 | 广东联鲲集团有限公司 | It is a kind of to improve the additive and its application that Pelteobagrus fulvidraco enteron aisle hardens |
| CN108606165B (en) * | 2018-04-19 | 2022-02-11 | 广东联鲲集团有限公司 | Additive for improving intestinal sclerosis of pelteobagrus fulvidraco and application thereof |
| CN108782955A (en) * | 2018-07-12 | 2018-11-13 | 漳州傲农牧业科技有限公司 | A kind of feed and preparation method thereof improving Pelteobagrus fulvidraco body colour and meat |
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