CN105265376A - Compound Chinese herbal medicine preparation capable of promoting multi-microecological preparation proliferation - Google Patents
Compound Chinese herbal medicine preparation capable of promoting multi-microecological preparation proliferation Download PDFInfo
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- CN105265376A CN105265376A CN201510584274.4A CN201510584274A CN105265376A CN 105265376 A CN105265376 A CN 105265376A CN 201510584274 A CN201510584274 A CN 201510584274A CN 105265376 A CN105265376 A CN 105265376A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 241000411851 herbal medicine Species 0.000 title claims abstract description 26
- 230000001737 promoting effect Effects 0.000 title claims abstract description 10
- 230000035755 proliferation Effects 0.000 title abstract description 7
- 150000001875 compounds Chemical class 0.000 title abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 28
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 13
- 235000018291 probiotics Nutrition 0.000 claims description 25
- 239000006041 probiotic Substances 0.000 claims description 23
- 238000000338 in vitro Methods 0.000 claims description 19
- MUKYLHIZBOASDM-UHFFFAOYSA-N 2-[carbamimidoyl(methyl)amino]acetic acid 2,3,4,5,6-pentahydroxyhexanoic acid Chemical compound NC(=N)N(C)CC(O)=O.OCC(O)C(O)C(O)C(O)C(O)=O MUKYLHIZBOASDM-UHFFFAOYSA-N 0.000 claims description 17
- 241000208688 Eucommia Species 0.000 claims description 17
- 241000830535 Ligustrum lucidum Species 0.000 claims description 17
- 244000241838 Lycium barbarum Species 0.000 claims description 17
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 17
- 241001533104 Tribulus terrestris Species 0.000 claims description 17
- 239000010231 banlangen Substances 0.000 claims description 17
- 235000013399 edible fruits Nutrition 0.000 claims description 17
- 229940079593 drug Drugs 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000002994 raw material Substances 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- 241000194108 Bacillus licheniformis Species 0.000 claims description 8
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 8
- 241000194032 Enterococcus faecalis Species 0.000 claims description 8
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 8
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 8
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 8
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 8
- 235000013557 nattō Nutrition 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 6
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- 239000000706 filtrate Substances 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 13
- 239000000126 substance Substances 0.000 abstract description 6
- 238000009360 aquaculture Methods 0.000 abstract description 5
- 208000010824 fish disease Diseases 0.000 abstract description 4
- 244000144974 aquaculture Species 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 206010034133 Pathogen resistance Diseases 0.000 abstract description 2
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 241000276707 Tilapia Species 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 238000007792 addition Methods 0.000 description 8
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
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- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
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- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000276701 Oreochromis mossambicus Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
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- 230000002155 anti-virotic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
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- 230000001079 digestive effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
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- 238000005457 optimization Methods 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of compound Chinese herbal medicine, and particularly discloses a compound Chinese herbal medicine preparation capable of promoting multi-microecological preparation proliferation. Chinese herbal medicine types with proliferation effects on microecological preparations such as multiple type of bacillus and lactobacillus are selected firstly, reasonable combination is then carried out on the Chinese herbal medicine, effects of promoting proliferation of multiple microecological preparations at the same time are thus achieved, multiple Chinese herbal medicine microecological preparations are acquired, and when the preparation are applied to the aquaculture industry, hidden safety problems such as polluted environment, bacterial resistance and residual medicine when antibiotics and chemical medicine are used for preventing and treating fish diseases can be effectively solved, and an important role is played in promoting healthy and sustainable development of the Tilapia culture industry.
Description
Technical field
The present invention relates to compound Chinese herb technical field, particularly, relate to a kind of Chinese herbal formula promoting multiple probiotics in-vitro multiplication.
Background technology
Along with the expansion of cultured area and the raising of cultivation density, the disease problem of fish is on the rise.At present, when preventing and curing of fish disease, the application of antibiotic and chemicals is still commonplace, Long-Time Service antibiotic and chemicals, fish body can be made to develop immunity to drugs and medicament residue, affect the sound development of culture fishery.Modern medicine study shows, the antibiotic residue in animal food likely makes human DNA structure change, thus causes the serious consequence of " disabling ", " teratogenesis ", " carcinogenic ".Probio is had by the effect providing nutriment or produce digestive ferment raising body digestion power, suppressed harmful microorganism to be bred by competition nutrition attachment site or generation antagonistic substance, improved water quality, improved resistance against diseases, strengthen the aspects such as anti-virus ability by enhanced machine body immunity function, is widely used in aquaculture as Substitutes For Antibiotic.Extensive application development test shows: the probioticses such as bacillus, Bacillus acidi lactici, nitrifying bacteria of splashing in the cultivating pool of eutrophication, probiotics can utilize the ammonia in breeding water body, nitrogen, nitrite etc. to have the material of damaging effect as nutritive element to healthy fish, carries out growth and breeding.Probiotics by purifying water, with the mode such as pathogen, harmful algae competitive growth space, the growth and breeding of pathogen and harmful algae can not only be suppressed, control the generation of fish diseases, effectively can also avoid because of Long-Time Service antibiotic and chemicals, cause fish body to develop immunity to drugs and the problem such as medicament residue.
Chinese herbal medicine and probio have synergistic effect.Contained flavones in Chinese herbal medicine, saponin, nucleotide, vitamin, folic acid, polysaccharide isoreactivity composition are the important growth factors of probio, can promote the propagation of probiotics.Probio can produce the multiple enzymes such as vitamin, amino acid, somatomedin, protease, lipase, cellulase, can improve absorption and the availability of Chinese herbal medicine, promotes the growth of aquiculture animal.Chinese herbal medicine and probiotic combination use, and are better than being used alone Chinese herbal medicine or probio to the growth of aquiculture animal and premunition impact effect.At present, the Chinese herbal medicine that the proliferation function research of research Chinese herbal medicine to probio both at home and abroad focuses mostly in single kind is to the proliferation function of single kind probio, and the cultivation effect research of compound Chinese herb to probio is also comparatively rare.Reported for work about compound Chinese herb also there is not yet research at present to the proliferation research of multiple probio.
Summary of the invention
The present invention, in order to overcome the above-mentioned deficiency of prior art, provides a kind of Chinese herbal formula promoting multiple probiotics in-vitro multiplication.
To achieve these goals, the present invention is achieved by the following technical programs:
Promote a Chinese herbal formula for multiple probiotics in-vitro multiplication, the raw material components containing following parts by weight: wilsonii 25 ~ 40 parts, the bark of eucommia 20 ~ 40 parts, matrimony vine 20 ~ 40 parts, puncture vine 5 ~ 20 parts, the fruit of glossy privet 5 ~ 20 parts, Radix Isatidis 5 ~ 20 parts, 5 ~ 15 parts, Radix Glycyrrhizae.
As composition of preferably filling a prescription, the Chinese herbal formula of the multiple probiotics in-vitro multiplication of described promotion, the raw material components containing following parts by weight: wilsonii 35 ~ 38 parts, the bark of eucommia 30 ~ 35 parts, matrimony vine 30 ~ 35 parts, puncture vine 10 ~ 15 parts, the fruit of glossy privet 10 ~ 15 parts, Radix Isatidis 10 ~ 15 parts, 6 ~ 15 parts, Radix Glycyrrhizae.
As most preferred formula composition, the Chinese herbal formula of the multiple probiotics in-vitro multiplication of described promotion, the raw material components containing following parts by weight: wilsonii 38 parts, the bark of eucommia 33 parts, matrimony vine 30 parts, puncture vine 10 parts, the fruit of glossy privet 12 parts, Radix Isatidis 10 parts, 6 parts, Radix Glycyrrhizae.
Chinese herbal and crude drugs preparations described above is promoting the application in multiple probiotics in-vitro multiplication.
The present invention is found by research, Chinese herbal and crude drugs preparations of the present invention can promote the in-vitro multiplication of bacillus and Bacillus acidi lactici simultaneously, to the bafillus natto in bacillus, bacillus subtilis, bacillus licheniformis, the facilitation highly significant of the lactobacillus acidophilus in Bacillus acidi lactici, enterococcus faecalis.Preferably, described multiple probiotics is bacillus and Bacillus acidi lactici.More preferably, described multiple probiotics is lactobacillus acidophilus, enterococcus faecalis, bafillus natto, bacillus subtilis, bacillus licheniformis.
Chinese herbal formula of the present invention is easy to use, and the Chinese herbal formula adding 5% in the medium significantly can promote the in-vitro multiplication of lactobacillus acidophilus, enterococcus faecalis and bafillus natto; Add 8% Chinese herbal formula in the medium and significantly can promote the in-vitro multiplication that bacillus subtilis and bacillus licheniformis come.
The preparation method of Chinese herbal and crude drugs preparations described above, comprises the following steps:
S1. by wilsonii, the bark of eucommia, matrimony vine, puncture vine, the fruit of glossy privet, Radix Isatidis, Radix Glycyrrhizae coarse crushing respectively, after crossing 20 mesh sieves, mix in proportion;
S2. in the Chinese herbal medicine of mixing, add appropriate distilled water immersion 10 ~ 30min, be heated to boiling, after slow fire boils 30 ~ 60min, filter, filter residue adds water and decocts once again, merges twice filtrate, by evaporation and concentration, sterilizing, obtained former medicine content is the Chinese herbal formula of 0.5 ~ 1.0g/mL.
Preferably, described in step S2, obtained former medicine content is the Chinese herbal formula of 0.5g/mL.
Compared with prior art, the present invention has following beneficial effect:
The present invention first filters out the Chinese herbal medicine kind probioticses such as multiple bacillus, Bacillus acidi lactici being had to in-vitro multiplication effect, again these Chinese herbal medicines are carried out reasonable compatibility, thus reach the effect that simultaneously can promote multiple probiotics in-vitro multiplication, obtain multiple Chinese herbal microecological preparation, be applied in culture fishery, effectively can solve and use the chemicals preventing and curing of fish disease of the raw element of protest to there is the security hidden troubles such as contaminated environment, bacterial resistance, medicament residue, significant to the health and sustainable development promoting Tilapia mossambica aquaculture.
Accompanying drawing explanation
Fig. 1 is that the Chinese herbal formula for preparing of embodiment 1 ~ 4 is to the cultivation effect of 5 kinds of probios; Wherein, medium based on blue pillar, the Chinese herbal and crude drugs preparations of medium+embodiment 1 based on red pillar; The Chinese herbal and crude drugs preparations of medium+embodiment 2 based on yellow pillar; The Chinese herbal and crude drugs preparations of medium+embodiment 3 based on green pillar; The Chinese herbal formula of medium+embodiment 4 based on the pillar of purple.
Fig. 2 is the optimization of Chinese herbal formula addition described in embodiment 4.
Fig. 3 is for Chinese herbal formula described in embodiment 4 is to the external increment Detection of colony result of 5 kinds of probios.
Fig. 4 is the in-vitro multiplication effect of each folk prescription Chinese herbal and crude drugs preparations to 5 kinds of probios.
Embodiment
To make the present invention below in conjunction with Figure of description and specific embodiment and elaborating further, described embodiment, only for explaining the present invention, is not intended to limit scope of the present invention.The test method used in following embodiment if no special instructions, is conventional method; The material used, reagent etc. if no special instructions, are the reagent that can obtain from commercial channels and material.
embodiment 1
Promote a Chinese herbal formula for multiple probiotics in-vitro multiplication, the raw material components containing following parts by weight: wilsonii 35 parts, the bark of eucommia 35 parts, matrimony vine 35 parts, puncture vine 15 parts, the fruit of glossy privet 15 parts, Radix Isatidis 15 parts, 15 parts, Radix Glycyrrhizae.
The preparation method of Chinese herbal formula is as follows:
S1. by the coarse crushing respectively of the Chinese herbal medicines such as wilsonii, the bark of eucommia, matrimony vine, puncture vine, the fruit of glossy privet, Radix Isatidis, Radix Glycyrrhizae, mix according to the above ratio after crossing 20 mesh sieves;
S2. in the Chinese herbal medicine of mixing, add 2000mL distilled water immersion 30min, be heated to boiling, after slow fire boils 30min, filter, filter residue adds water and decocts once again, merges twice filtrate, by evaporation and concentration, sterilizing, obtained former medicine content is the Chinese herbal formula of 0.5g/mL.
embodiment 2
Promote a Chinese herbal formula for multiple probiotics in-vitro multiplication, the raw material components containing following parts by weight: wilsonii 35, the bark of eucommia 35, matrimony vine 30, puncture vine 10, the fruit of glossy privet 10, Radix Isatidis 10, Radix Glycyrrhizae 10.
The preparation method of Chinese herbal formula is as follows:
S1. by the coarse crushing respectively of the Chinese herbal medicines such as wilsonii, the bark of eucommia, matrimony vine, puncture vine, the fruit of glossy privet, Radix Isatidis, Radix Glycyrrhizae, mix according to the above ratio after crossing 20 mesh sieves;
S2. in the Chinese herbal medicine of mixing, add the distilled water immersion 30min of 2000mL, be heated to boiling, after slow fire boils 30min, filter, filter residue adds water and decocts once again, merges twice filtrate, by evaporation and concentration, sterilizing, obtained former medicine content is the Chinese herbal formula of 0.5g/mL.
embodiment 3
Promote a Chinese herbal formula for multiple probiotics in-vitro multiplication, the raw material components containing following percetage by weight: wilsonii 38, the bark of eucommia 35, matrimony vine 30, puncture vine 15, the fruit of glossy privet 15, Radix Isatidis 10, Radix Glycyrrhizae 10.
The preparation method of Chinese herbal formula is as follows:
S1. by the coarse crushing respectively of the Chinese herbal medicines such as wilsonii, the bark of eucommia, matrimony vine, puncture vine, the fruit of glossy privet, Radix Isatidis, Radix Glycyrrhizae, mix according to the above ratio after crossing 20 mesh sieves;
S2. in the Chinese herbal medicine of mixing, add the distilled water immersion 30min of 2000mL, be heated to boiling, after slow fire boils 30min, filter, filter residue adds water and decocts once again, merges twice filtrate, by evaporation and concentration, sterilizing, obtained former medicine content is the Chinese herbal formula of 0.5g/mL.
embodiment 4
Promote a Chinese herbal formula for multiple probiotics in-vitro multiplication, the raw material components containing following percetage by weight: wilsonii 38, the bark of eucommia 33, matrimony vine 30, puncture vine 10, the fruit of glossy privet 12, Radix Isatidis 10, Radix Glycyrrhizae 6.
The preparation method of Chinese herbal formula is as follows:
S1. by the coarse crushing respectively of the Chinese herbal medicines such as wilsonii, the bark of eucommia, matrimony vine, puncture vine, the fruit of glossy privet, Radix Isatidis, Radix Glycyrrhizae, mix according to the above ratio after crossing 20 mesh sieves;
S2. in the Chinese herbal medicine of mixing, add the distilled water immersion 30min of 2000mL, be heated to boiling, after slow fire boils 30min, filter, filter residue adds water and decocts once again, merges twice filtrate, by evaporation and concentration, sterilizing, obtained former medicine content is the Chinese herbal formula of 0.5g/mL.
application examples 1
The Chinese herbal formula that embodiment 1 ~ 4 prepares is used for cultivate 5 kinds of probios, lactobacillus acidophilus, enterococcus faecalis, bafillus natto, bacillus subtilis and bacillus licheniformis.Embody rule is as follows:
(1) actication of culture: get three bacillus bacterial classification (bafillus natto, bacillus subtilis and bacillus licheniformis, there is provided by Guangdong Lubaiduo Bio-tech Co., Ltd.) be inoculated in nutrient broth with the inoculum concentration of 1% respectively, then shaking table is placed in, with 37 DEG C, cultivate 1 day under the condition of 200r/min, more for subsequent use after carrying out re-activation 12h.Lactic acid bacteria (lactobacillus acidophilus, enterococcus faecalis, provided by Guangdong Lubaiduo Bio-tech Co., Ltd.) with in the inoculum concentration access MRS liquid nutrient medium of 1%, cultivates 48h under 37 DEG C of anaerobic conditions respectively, more for subsequent use after carrying out re-activation 24h.
(2) Chinese herbal formula that embodiment 1 ~ 4 prepares is added to respectively in the culture fluid of above-mentioned 5 kinds of bacterial strains, the addition of the Chinese herbal formula that embodiment 1 ~ 4 prepares in lactobacillus acidophilus, enterococcus faecalis and bafillus natto culture fluid is 5%, and the addition in bacillus subtilis, bacillus licheniformis are cultivated is 8%.The cultivation effect of the Chinese herbal formula that embodiment 1 ~ 4 prepares to several probiotics is shown in Fig. 1.As shown in Figure 1, the external value-added effect of Chinese herbal formula to 5 kinds of probios that prepare of embodiment 4 is best.
embodiment 5
Because the external value-added effect of the Chinese herbal formula that embodiment 4 prepares to 5 kinds of probios is best, so, the consumption of inventor to the Chinese herbal formula of embodiment 4 is optimized, be provided with 3 kinds of additions respectively, 2%, 5% and 8%, Different adding amount the results are shown in Figure 2, as shown in Figure 2, the optimum addition of Chinese herbal formula in lactobacillus acidophilus, enterococcus faecalis and bafillus natto culture fluid of embodiment 4 is 5%, and the optimum addition in bacillus subtilis, bacillus licheniformis are cultivated is 8%.
The Chinese herbal formula of embodiment 4 is added to according to optimum addition in the medium of 5 kinds of probios respectively, the same application examples of concrete operations, cultivate after terminating, measure the colony count of 5 kinds of probios, the results are shown in Figure 3.
embodiment 6
By the preparation method of the 7 taste Chinese herbal medicines such as wilsonii, the bark of eucommia, matrimony vine, puncture vine, the fruit of glossy privet, Radix Isatidis, Radix Glycyrrhizae according to embodiment 4, be prepared into folk prescription Chinese herbal and crude drugs preparations respectively, then according to 10% addition add above-mentioned 5 kinds of probios culture fluid in, measure the external value-added effect of each folk prescription Chinese herbal and crude drugs preparations to 5 kinds of probios, the results are shown in Figure 4.
Claims (7)
1. one kind promotes the Chinese herbal formula of multiple probiotics in-vitro multiplication, it is characterized in that, the raw material components containing following parts by weight: wilsonii 25 ~ 40 parts, the bark of eucommia 20 ~ 40 parts, matrimony vine 20 ~ 40 parts, puncture vine 5 ~ 20 parts, the fruit of glossy privet 5 ~ 20 parts, Radix Isatidis 5 ~ 20 parts, 5 ~ 15 parts, Radix Glycyrrhizae.
2. Chinese herbal and crude drugs preparations according to claim 1, it is characterized in that, the raw material components containing following parts by weight: wilsonii 35 ~ 38 parts, the bark of eucommia 30 ~ 35 parts, matrimony vine 30 ~ 35 parts, puncture vine 10 ~ 15 parts, the fruit of glossy privet 10 ~ 15 parts, Radix Isatidis 10 ~ 15 parts, 6 ~ 15 parts, Radix Glycyrrhizae.
3. Chinese herbal and crude drugs preparations according to claim 1, is characterized in that, the raw material components containing following parts by weight: wilsonii 38 parts, the bark of eucommia 33 parts, matrimony vine 30 parts, puncture vine 10 parts, the fruit of glossy privet 12 parts, Radix Isatidis 10 parts, 6 parts, Radix Glycyrrhizae.
4. Chinese herbal and crude drugs preparations described in any one of claims 1 to 3 is promoting the application in multiple probiotics in-vitro multiplication.
5. application according to claim 4, is characterized in that, described multiple probiotics is bacillus and lactic acid bacteria.
6. application according to claim 4, is characterized in that, described multiple probiotics is lactobacillus acidophilus, enterococcus faecalis, bafillus natto, bacillus subtilis, bacillus licheniformis.
7. the preparation method of Chinese herbal and crude drugs preparations described in any one of claims 1 to 3, is characterized in that, comprise the steps:
S1. by wilsonii, the bark of eucommia, matrimony vine, puncture vine, the fruit of glossy privet, Radix Isatidis, Radix Glycyrrhizae coarse crushing respectively, after crossing 20 mesh sieves, mix in proportion;
S2. in the Chinese herbal medicine of mixing, add appropriate distilled water immersion 10 ~ 30min, be heated to boiling, after slow fire boils 30 ~ 60min, filter, filter residue adds water and decocts once again, merges twice filtrate, by evaporation and concentration, sterilizing, obtained former medicine content is the Chinese herbal formula of 0.5 ~ 1.0g/mL.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106222125A (en) * | 2016-09-14 | 2016-12-14 | 光明乳业股份有限公司 | A kind of Lactobacillus plantarum multiplication agent and application thereof |
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| CN107752039A (en) * | 2017-11-21 | 2018-03-06 | 常州市阿曼特化工有限公司 | A kind of preparation method of probiotics microbial inoculum |
| CN112011483A (en) * | 2020-08-13 | 2020-12-01 | 山东天润和生物工程有限公司 | Culture method suitable for enterococcus faecalis enrichment |
| CN112011483B (en) * | 2020-08-13 | 2022-07-19 | 山东天润和生物工程有限公司 | Culture method suitable for enterococcus faecalis enrichment |
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| CN105265376B (en) | 2018-10-19 |
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