CN115633735A - Plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity - Google Patents
Plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention belongs to the technical field of feed additives, and discloses a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity, which comprises, by mass, 15-20 parts of mulberry leaf powder, 15-20 parts of pine needle powder, 10-15 parts of isatis root extract, 10-15 parts of dandelion, 8-10 parts of astragalus extract, 8-10 parts of honeysuckle extract, 6-8 parts of sweet wormwood herb, 3-6 parts of fried radix bupleuri, 5-8 parts of radix pseudostellariae extract, 5-8 parts of sea buckthorn extract, 5-8 parts of garlic extract, 3-6 parts of inulin, 3-6 parts of fermented apple pomace, 1-3 parts of compound microbial inoculant, 1-3 parts of compound vitamin and 1-3 parts of trace elements. The invention can improve the integral disease prevention and resistance of the pork pig and promote the balanced and healthy growth of the pork pig on the premise of not reducing the existing growth speed.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity.
Background
At present, with the development of animal nutrition and feed industry, the residue of chemical products or biological products such as veterinary drugs, antibiotics, growth promoters and the like used as feed additives in meat and edible viscera seriously endangers the eating safety of human beings, especially children; although the growth rate is satisfactory, the occurrence of various diseases and epidemic diseases is endless, especially the number of respiratory diseases is increased obviously, and the traditional typical symptoms do not exist. The overall cost (disease prevention, disease treatment, growth promotion and the like) of pig raising production is getting larger and larger, and not only the overall cost is increased, but also the stress myopathy proportion of meat of the slaughtered pork is increased continuously (the stress myopathy refers to diseases characterized by various stresses before the slaughtered pork and skeletal muscle edema, degeneration, necrosis and inflammation which are caused by improper processing after the slaughtered pork, the common manifestations are that PSE pork (pork with water), dorsal muscle necrosis and leg muscle necrosis appear in the slaughtered pork, and the total cost is up to more than 60% in some regions; the existing method for preventing partial pig diseases by adding antibiotics into the pig feed additive can not improve the immunity of pigs, and can cause the reduction of the immunity of the pigs and the antibiotic residue in pork after long-term administration of the antibiotics. Therefore, a green and safe feed additive capable of promoting the enhancement of pig immunity and preventing diseases is urgently needed.
Through the above analysis, the problems and defects of the prior art are as follows: the conventional antibiotic or chemical additive cannot improve the immunity of the pigs, can cause the reduction of the immunity of the pigs and the stress myopathy of the pigs after long-term use of the antibiotic, and has the disadvantages of harm to health, insecurity and high cost because of the existence of antibiotic or chemical medicine residues in pork.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity.
The plant extract feed additive for preventing and treating the porcine respiratory disease and enhancing the immunity is prepared from 15-20 parts by mass of mulberry leaf powder, 15-20 parts by mass of pine needle powder, 10-15 parts by mass of isatis root extract, 10-15 parts by mass of dandelion, 8-10 parts by mass of astragalus extract, 8-10 parts by mass of honeysuckle extract, 6-8 parts by mass of sweet wormwood herb, 3-6 parts by mass of fried radix bupleuri, 5-8 parts by mass of radix pseudostellariae extract, 5-8 parts by mass of sea buckthorn extract, 5-8 parts by mass of garlic extract, 3-6 parts by mass of inulin, 3-6 parts by mass of fermented apple pomace, 1-3 parts by mass of composite microbial agent, 1-3 parts by mass of composite vitamin and 1-3 parts by mass of trace element;
the microbial agent consists of enterococcus, bifidobacterium, bacillus coagulans, bacillus subtilis and bacillus marinus;
the microelements comprise any one or more of zinc lactate, ferrous fumarate, cobalt methionine, copper glycinate, sodium selenite, chromium picolinate and amino acids.
The invention also aims to provide a preparation method of the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity, wherein the preparation method of the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity comprises the following steps:
step one, respectively taking mulberry leaves, pine needles and dandelion which have no pathological changes, removing impurities by using a screen, and then cleaning by using clear water to obtain clean mulberry leaves, pine needles and dandelion;
step two, drying the clean mulberry leaves, pine needles and dandelion respectively; respectively crushing the dried mulberry leaves, pine needles and dandelion by using a crusher, and sieving by using a sieve of 80-120 meshes to obtain mulberry leaf powder, pine needle powder and dandelion powder;
step three, taking healthy sweet wormwood, soaking the sweet wormwood for a period of time by using a sodium chloride solution, taking out the sweet wormwood, draining water, crushing and sieving to obtain sweet wormwood powder;
step four, preparing inulin by utilizing fresh jerusalem artichoke; selecting fresh garlic which is ripe and does not bud and rot, and preparing garlic extract; respectively preparing an isatis root extract, an astragalus extract, a honeysuckle extract, fried radix bupleuri, a radix pseudostellariae extract and a sea buckthorn extract;
weighing mulberry leaf powder, pine needle powder, an isatis root extract, dandelion powder, an astragalus extract, a honeysuckle extract, sweet wormwood powder, fried radix bupleuri, a radix pseudostellariae extract, a sea buckthorn extract, a garlic extract, inulin, fermented apple pomace, a compound microbial agent, compound vitamins and trace elements in proportion;
step six, uniformly mixing the weighed mulberry leaf powder, pine needle powder, isatis root extract, dandelion powder, astragalus extract, honeysuckle extract, sweet wormwood powder, fried radix bupleuri, radix pseudostellariae extract, sea buckthorn extract, garlic extract, inulin, fermented apple pomace and compound microbial agent, and then performing fermentation treatment to obtain a fermentation product;
and seventhly, filtering, drying and stopping fermentation of the obtained fermentation product, and then adding the weighed composite vitamins and trace elements to obtain the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity.
Further, the preparation of inulin by using fresh jerusalem artichoke in the fourth step comprises the following steps:
(1) Cleaning fresh jerusalem artichoke, and slicing to obtain jerusalem artichoke slices; soaking the Jerusalem artichoke slices in 75-95 deg.C hot water for 6-8 min, taking out, squeezing with a squeezing device, adding hot water, soaking, squeezing for one time, and mixing the two squeezed juices to obtain Jerusalem artichoke juice and Jerusalem artichoke residue;
(2) Oven drying Jerusalem artichoke residue, pulverizing, sieving with 80-100 mesh sieve, adding a certain amount of hot water into Jerusalem artichoke residue, and pressure heating for extracting to obtain Jerusalem artichoke residue primary extract; mixing the jerusalem artichoke residue primary extract with the jerusalem artichoke juice in the step (1) to obtain a jerusalem artichoke coarse extract;
(3) And purifying the obtained crude extract of the jerusalem artichoke to obtain inulin precipitate, and carrying out reduced pressure concentration and spray drying treatment on the inulin precipitate to obtain the inulin.
Further, the purification treatment of the obtained crude extract of jerusalem artichoke to obtain inulin precipitates comprises the following steps:
firstly, obtaining a jerusalem artichoke coarse extract, and carrying out water bath heating treatment on the obtained jerusalem artichoke extract; adding lime milk into the jerusalem artichoke extracting solution heated in water bath while stirring, adjusting the pH value of the jerusalem artichoke extracting solution to 12.5-13.5, and continuously stirring for 10 minutes;
secondly, trichloroacetic acid is added into the jerusalem artichoke extracting solution to adjust the pH value of the jerusalem artichoke extracting solution to 6.5; and simultaneously heating the jerusalem artichoke extracting solution to 80 ℃ while stirring, preserving the heat for 8 minutes, naturally cooling to room temperature, and filtering to obtain the inulin precipitate.
Further, the water bath heating treatment comprises: heating in water bath at 65-75 deg.c.
Further, the preparation of the garlic extract by selecting ripe, non-germinated and moldy fresh garlic comprises the following steps:
firstly, selecting mature, non-germinated and moldy fresh garlic, peeling the selected garlic, and soaking the garlic in a sodium chloride solution for a period of time; taking out the soaked garlic, and washing with flowing water for a period of time to obtain clean garlic;
secondly, adding clean garlic and a certain amount of alkaline deionized water into a pounding machine for pounding to obtain mashed garlic; adding alliinase into the mashed garlic, carrying out ultrasonic oscillation and uniform mixing, and carrying out enzymolysis for 30-60 minutes at normal temperature to obtain garlic enzymolysis liquid;
and finally, carrying out ultrasonic crushing treatment and centrifugal treatment on the garlic enzymolysis liquid, filtering by using an ultrafiltration membrane, retaining supernatant to obtain garlic extract liquid, and carrying out reduced pressure concentration on the garlic extract liquid by using a rotary evaporator to obtain the garlic extract.
Further, the ultrasonic pulverization treatment comprises: pulverizing under ultrasound at 400-600W for 15min.
Further, the centrifugation process comprises: centrifugation was carried out at 3000r/min at 10 ℃ for 15min.
The invention also aims to provide a plant extract feed for preventing and treating the porcine respiratory diseases and enhancing the immunity, which is added with the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity.
In combination with the technical solutions and the technical problems to be solved, please analyze the advantages and positive effects of the technical solutions to be protected in the present invention from the following aspects:
first, aiming at the technical problems existing in the prior art and the difficulty in solving the problems, the technical problems to be solved by the technical scheme of the present invention are closely combined with results, data and the like in the research and development process, and some creative technical effects are brought after the problems are solved. The specific description is as follows:
the invention adopts natural Chinese herbal medicines or extracts of plants as raw materials, so that the cost is low, the raw materials are easy to obtain, and the preparation process is simple; meanwhile, the feed additive can improve the overall disease prevention and resistance of the pork pigs on the premise of not reducing the existing growth speed, and promote the balanced and healthy growth of the pigs; promoting the organism to synthesize or absorb trace flavor development substances and effectively deposit, and improving the meat quality; reduce the occurrence of stress myopathy and produce high-quality pollution-free pork.
Secondly, considering the technical scheme as a whole or from the perspective of products, the technical effect and advantages of the technical scheme to be protected by the invention are specifically described as follows:
according to the invention, the plant extract feed additive for preventing and treating the pig respiratory diseases and enhancing the immunity is added into the daily ration of the growing pig, and the occurrence of the pig respiratory diseases can be greatly reduced by utilizing the synergistic cooperation of a plurality of plant extracts including the isatis root extract, the dandelion, the astragalus extract, the honeysuckle extract, the sweet wormwood herb, the fried radix bupleuri, the radix pseudostellariae extract, the sea buckthorn extract and the garlic extract, so that the immunity and the growth performance of the growing pig are effectively improved, the traditional antibiotics or additives can be replaced, the drug residue is reduced, and the breeding benefit is improved.
Drawings
FIG. 1 is a flow chart of a preparation method of a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to an embodiment of the invention;
FIG. 2 is a flow chart of a method for preparing inulin by using fresh jerusalem artichoke according to an embodiment of the invention;
FIG. 3 is a flow chart of the method for purifying the obtained Jerusalem artichoke crude extract to obtain inulin precipitate, which is provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
1. Illustrative embodiments are explained. This section is an explanatory embodiment expanding on the claims so as to fully understand how the present invention is embodied by those skilled in the art.
The plant extract feed additive for preventing and treating the porcine respiratory disease and enhancing the immunity, provided by the embodiment of the invention, comprises, by mass, 15-20 parts of mulberry leaf powder, 15-20 parts of pine needle powder, 10-15 parts of isatis root extract, 10-15 parts of dandelion, 8-10 parts of astragalus extract, 8-10 parts of honeysuckle extract, 6-8 parts of sweet wormwood herb, 3-6 parts of fried radix bupleuri, 5-8 parts of radix pseudostellariae extract, 5-8 parts of sea buckthorn extract, 5-8 parts of garlic extract, 3-6 parts of inulin, 3-6 parts of fermented apple pomace, 1-3 parts of composite microbial agent, 1-3 parts of composite vitamin and 1-3 parts of trace elements.
The microbial agent provided by the embodiment of the invention consists of enterococcus, bifidobacterium, bacillus coagulans, bacillus subtilis and bacillus marinus.
The trace elements provided by the embodiment of the invention consist of any one or more of zinc lactate, ferrous fumarate, cobalt methionine, copper glycinate, sodium selenite, chromium picolinate and amino acid.
As shown in fig. 1, the preparation method of the plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity provided by the embodiment of the invention comprises the following steps:
s101, respectively taking the mulberry leaves, the pine needles and the dandelions without pathological changes, removing impurities by using a screen, and then cleaning by using clear water to obtain clean mulberry leaves, pine needles and dandelions;
s102, drying clean mulberry leaves, pine needles and dandelion respectively; crushing the dried mulberry leaves, pine needles and dandelion by using a crusher respectively, and sieving by using a sieve of 80-120 meshes to obtain mulberry leaf powder, pine needle powder and dandelion powder;
s103, taking healthy sweet wormwood, soaking the sweet wormwood for a period of time by using a sodium chloride solution, taking out the sweet wormwood, draining water, crushing and sieving to obtain sweet wormwood powder;
s104, preparing inulin by using fresh jerusalem artichoke; selecting fresh garlic which is ripe and does not bud and rot, and preparing garlic extract; respectively preparing radix Isatidis extract, radix astragali extract, flos Lonicerae extract, parched bupleuri radix, radix Pseudostellariae extract, and fructus Hippophae extract;
s105, weighing mulberry leaf powder, pine needle powder, an isatis root extract, dandelion powder, an astragalus extract, a honeysuckle extract, sweet wormwood powder, fried radix bupleuri, a radix pseudostellariae extract, a sea buckthorn extract, a garlic extract, inulin, fermented apple pomace, a compound microbial agent, compound vitamins and trace elements in proportion;
s106, uniformly mixing the weighed mulberry leaf powder, pine needle powder, isatis root extract, dandelion powder, astragalus extract, honeysuckle extract, sweet wormwood powder, fried radix bupleuri, radix pseudostellariae extract, sea buckthorn extract, garlic extract, inulin, fermented apple pomace and compound microbial agent, and then performing fermentation treatment to obtain a fermented product;
s107, filtering, drying and stopping fermentation of the obtained fermentation product, adding the compound vitamins and the trace elements, and uniformly mixing to obtain the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity.
As shown in FIG. 2, the preparation of inulin from fresh Jerusalem artichoke according to the embodiment of the invention comprises the following steps:
s201, cleaning fresh jerusalem artichoke, and slicing to obtain jerusalem artichoke slices; soaking the Jerusalem artichoke slices in 75-95 deg.C hot water for 6-8 min, taking out, squeezing with a squeezing device, adding hot water, soaking, squeezing for one time, and mixing the two squeezed juices to obtain Jerusalem artichoke juice and Jerusalem artichoke residue;
s202, drying and crushing the jerusalem artichoke residues, sieving the jerusalem artichoke residues by a sieve with 80-100 meshes, adding a certain amount of hot water into the jerusalem artichoke residues, and carrying out pressurized heating and extraction to obtain an initial extract of the jerusalem artichoke residues; mixing the jerusalem artichoke residue primary extract with the jerusalem artichoke juice in the step S201 to obtain a jerusalem artichoke crude extract;
s203, purifying the obtained crude extract of the jerusalem artichoke to obtain inulin precipitates, and carrying out reduced pressure concentration and spray drying treatment on the inulin precipitates to obtain the inulin.
As shown in fig. 3, the purification treatment of the obtained crude extract of jerusalem artichoke according to the embodiment of the present invention to obtain inulin precipitates includes:
s301, obtaining a jerusalem artichoke crude extract, and heating the obtained jerusalem artichoke extract in a water bath at 65-75 ℃; adding lime milk into the jerusalem artichoke extracting solution heated in water bath under stirring, adjusting the pH value of the jerusalem artichoke extracting solution to 12.5-13.5, and continuously stirring for 10 minutes;
s302, adding trichloroacetic acid into the jerusalem artichoke extracting solution to adjust the pH value of the jerusalem artichoke extracting solution to 6.5; and simultaneously heating the jerusalem artichoke extracting solution to 80 ℃ while stirring, preserving the heat for 8 minutes, naturally cooling to room temperature, and filtering to obtain the inulin precipitate.
The preparation method of the garlic extract by selecting the fresh garlic which is ripe, ungerminated and does not have rotten mildew provided by the embodiment of the invention comprises the following steps:
firstly, selecting mature, non-germinated and moldy fresh garlic, peeling the selected garlic, and soaking the garlic in a sodium chloride solution for a period of time; taking out the soaked garlic, and washing with flowing water for a period of time to obtain clean garlic;
secondly, adding clean garlic and a certain amount of alkaline deionized water into a pounding machine for pounding to obtain mashed garlic; adding alliinase into the mashed garlic, carrying out ultrasonic oscillation and uniform mixing, and carrying out enzymolysis for 30-60 minutes at normal temperature to obtain garlic enzymolysis liquid;
and finally, carrying out ultrasonic crushing on the garlic enzymolysis liquid for 15 minutes under 400-600W, then centrifuging for 15 minutes at 10 ℃ at 3000r/min, filtering by using an ultrafiltration membrane, retaining supernatant to obtain a garlic extract extracting solution, and carrying out reduced pressure concentration on the garlic extract extracting solution by using a rotary evaporator to obtain the garlic extract.
2. Application examples. In order to prove the creativity and the technical value of the technical scheme of the invention, the part is an application example of the technical scheme of the claims to a specific product or related technology.
18 boars and sows with similar body conditions, good health conditions and similar ages are randomly divided into two groups for comparison experiments, the experimental group is fed with the pig ration added with the plant extract feed additive for preventing and treating the respiratory diseases and enhancing the immunity of the pigs, and the control group is fed with the common ration; the contrast experiment result proves that the growth speed of the test group is higher than that of the control group, and the contents of serum immunoglobulin IgG, igA, igM, complement C3 and C4 of the pigs in the test group are all far higher than those of the control group, so that the plant extract feed additive for preventing and treating the respiratory diseases of the pigs and enhancing the immunity provided by the application embodiment can effectively improve the immunity of the pigs.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. The plant extract feed additive for preventing and treating the porcine respiratory disease and enhancing the immunity is characterized by comprising, by mass, 15-20 parts of mulberry leaf powder, 15-20 parts of pine needle powder, 10-15 parts of isatis root extract, 10-15 parts of dandelion, 8-10 parts of astragalus extract, 8-10 parts of honeysuckle extract, 6-8 parts of sweet wormwood herb, 3-6 parts of fried radix bupleuri, 5-8 parts of radix pseudostellariae extract, 5-8 parts of sea buckthorn extract, 5-8 parts of garlic extract, 3-6 parts of inulin, 3-6 parts of fermented apple pomace, 1-3 parts of compound microbial inoculant, 1-3 parts of compound vitamin and 1-3 parts of trace elements;
the microbial agent consists of enterococcus, bifidobacterium, bacillus coagulans, bacillus subtilis and bacillus marinus;
the microelements comprise any one or more of zinc lactate, ferrous fumarate, cobalt methionine, copper glycinate, sodium selenite, chromium picolinate and amino acids.
2. The preparation method of the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity, which is used as the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity according to claim 1, is characterized in that the preparation method of the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity comprises the following steps:
step one, respectively taking mulberry leaves, pine needles and dandelion which have no pathological changes, removing impurities by using a screen, and then cleaning by using clear water to obtain clean mulberry leaves, pine needles and dandelion;
step two, drying the clean mulberry leaves, pine needles and dandelion respectively; crushing the dried mulberry leaves, pine needles and dandelion by a crusher respectively, and sieving to obtain mulberry leaf powder, pine needle powder and dandelion powder;
step three, taking healthy sweet wormwood, soaking the sweet wormwood for a period of time by using a sodium chloride solution, taking out the sweet wormwood, draining water, crushing and sieving to obtain sweet wormwood powder;
step four, preparing inulin by utilizing fresh jerusalem artichoke; selecting ripe and ungerminated fresh garlic and performing preparation on the garlic extract; respectively preparing radix Isatidis extract, radix astragali extract, flos Lonicerae extract, parched bupleuri radix, radix Pseudostellariae extract, and fructus Hippophae extract;
weighing mulberry leaf powder, pine needle powder, an isatis root extract, dandelion powder, an astragalus extract, a honeysuckle extract, sweet wormwood powder, fried radix bupleuri, a radix pseudostellariae extract, a sea buckthorn extract, a garlic extract, inulin, fermented apple pomace, a compound microbial agent, compound vitamins and trace elements in proportion;
step six, uniformly mixing the weighed mulberry leaf powder, pine needle powder, isatis root extract, dandelion powder, astragalus extract, honeysuckle extract, sweet wormwood powder, fried radix bupleuri, radix pseudostellariae extract, sea buckthorn extract, garlic extract, inulin, fermented apple pomace and compound microbial agent, and then performing fermentation treatment to obtain a fermentation product;
and seventhly, filtering, drying and stopping fermentation of the obtained fermentation product, and then adding the weighed composite vitamins and trace elements to obtain the plant extract feed additive for preventing and treating the porcine respiratory diseases and enhancing the immunity.
3. The method for preparing a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to claim 2, wherein in the second step, the sieving comprises: sieving with 80-120 mesh sieve.
4. The method for preparing a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to claim 2, wherein the step four of preparing inulin from fresh jerusalem artichoke comprises the following steps:
(1) Cleaning fresh jerusalem artichoke, and slicing to obtain jerusalem artichoke slices; soaking the Jerusalem artichoke slices in 75-95 deg.C hot water for 6-8 min, taking out, squeezing with a squeezing device, adding hot water, soaking, squeezing for one time, and mixing the two squeezed juices to obtain Jerusalem artichoke juice and Jerusalem artichoke residue;
(2) Oven drying Jerusalem artichoke residue, pulverizing, sieving with 80-100 mesh sieve, adding a certain amount of hot water into Jerusalem artichoke residue, and pressure heating for extracting to obtain Jerusalem artichoke residue primary extract; mixing the jerusalem artichoke primary extract with the jerusalem artichoke juice in the step (1) to obtain a jerusalem artichoke crude extract;
(3) And purifying the obtained crude extract of the jerusalem artichoke to obtain inulin precipitates, and carrying out reduced pressure concentration and spray drying treatment on the inulin precipitates to obtain the inulin.
5. The method for preparing a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to claim 4, wherein the purifying the obtained crude extract of jerusalem artichoke to obtain inulin precipitates comprises:
firstly, obtaining a jerusalem artichoke crude extract, and carrying out water bath heating treatment on the obtained jerusalem artichoke extract; adding lime milk into the jerusalem artichoke extracting solution heated in water bath while stirring, adjusting the pH value of the jerusalem artichoke extracting solution to 12.5-13.5, and continuously stirring for 10 minutes;
secondly, trichloroacetic acid is added into the jerusalem artichoke extracting solution to adjust the pH value of the jerusalem artichoke extracting solution to 6.5; and simultaneously heating the jerusalem artichoke extracting solution to 80 ℃ while stirring, preserving the heat for 8 minutes, naturally cooling to room temperature, and filtering to obtain the inulin precipitate.
6. The method for preparing a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to claim 4, wherein the water bath heating treatment comprises: heating in water bath at 65-75 deg.c.
7. The method for preparing a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to claim 2, wherein the selecting of ripe, ungerminated and moldy-free fresh garlic for the preparation of the garlic extract comprises:
firstly, selecting mature, non-germinated and moldy fresh garlic, peeling the selected garlic, and soaking the garlic in a sodium chloride solution for a period of time; taking out the soaked garlic, and washing with flowing water for a period of time to obtain clean garlic;
secondly, adding clean garlic and a certain amount of alkaline deionized water into a pounding machine for pounding to obtain mashed garlic; adding alliinase into the mashed garlic, carrying out ultrasonic oscillation and uniform mixing, and carrying out enzymolysis for 30-60 minutes at normal temperature to obtain garlic enzymolysis liquid;
and finally, carrying out ultrasonic crushing treatment and centrifugal treatment on the garlic enzymolysis liquid, filtering by using an ultrafiltration membrane, retaining supernatant to obtain garlic extract liquid, and carrying out reduced pressure concentration on the garlic extract liquid by using a rotary evaporator to obtain the garlic extract.
8. The method for preparing a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to claim 7, wherein the ultrasonic pulverization treatment comprises: pulverizing under ultrasound at 400-600W for 15min.
9. The method for preparing a plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to claim 7, wherein the centrifugation comprises: centrifugation was carried out at 3000r/min for 15min at 10 ℃.
10. A plant extract feed for preventing and treating porcine respiratory diseases and enhancing immunity, which is added with the plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity according to claim 1.
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