CN111034855A - Composition for enhancing respiratory tract immunity of animals, method and application thereof - Google Patents

Composition for enhancing respiratory tract immunity of animals, method and application thereof Download PDF

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Publication number
CN111034855A
CN111034855A CN201911407185.7A CN201911407185A CN111034855A CN 111034855 A CN111034855 A CN 111034855A CN 201911407185 A CN201911407185 A CN 201911407185A CN 111034855 A CN111034855 A CN 111034855A
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traditional chinese
chinese medicine
compound
respiratory tract
immunity
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张婵娟
闫凌鹏
麻啸涛
杨京娥
党亚朋
胡红伟
李薇
权豪强
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Shanxi Dayu Bioengineering Co ltd
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Shanxi Dayu Bioengineering Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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Abstract

The invention provides a composition for enhancing respiratory tract immunity of animals, which consists of a feed mixing additive added into feed and a spray composite bacteria preparation sprayed on poultry houses. The raw materials of the mixing additive comprise: 70-95 parts of compound fermentation traditional Chinese medicine, 0.2-0.5 part of vitamin A, 4-8 parts of vitamin C and 1-2 parts of vitamin E. Wherein the compound fermentation traditional Chinese medicine comprises: licorice root: mulberry leaf: mint: bitter apricot seeds: honeysuckle flower: astragalus membranaceus 2:1:1:1: 1. The spraying composite bacterium preparation comprises the following raw materials: b, bacillus subtilis: b, bacillus licheniformis: lactobacillus plantarum: enterococcus faecalis 1:1:1:1, and the spray concentration is 2mL/m3. According to the particularity of the respiratory tract mucosa, the invention adopts two modes of material mixing and spraying to play a role together, thereby enhancing the immunologic function of the respiratory tract mucosa and comprehensively preventing and treating the respiratory tract diseases of animals.

Description

Composition for enhancing respiratory tract immunity of animals, method and application thereof
Technical Field
The invention relates to the field of feed additives, in particular to a composition for enhancing respiratory tract immunity of animals, a method and application thereof.
Background
In recent years, along with the rapid increase of the scale field, the respiratory diseases of livestock and poultry are more and more emphasized, and the prevention and treatment of the respiratory diseases are more and more important. For example, mycoplasma infection is a troublesome disease in animal breeding. After mycoplasma infection, on one hand, respiratory epithelial cells and villi cells are damaged, and the barrier effect of respiratory mucosa is reduced, and on the other hand, the immune system of the respiratory tract is damaged, so that the sensitivity of the organism to various pathogenic bacteria and viruses is increased, and secondary infection is caused.
In addition to systemic immune function, the animal body also includes local immunity of the upper respiratory tract and digestive tract mucosa. The two are natural portals of the body, protect the respiratory tract and intestinal tract immunity, and can block the invasion of pathogenic bacteria. At present, measures for improving respiratory mucosa and enhancing respiratory immunity mainly comprise the following measures: A. strengthen raising management, improve the environment of raising: harmful gas, feed dust particles, pathogenic microorganisms and the like in the environment of the animal house can cause great threat to respiratory tract mucosa of animals, good feeding density is ensured, the feeding density is maintained at 30-33kg/m2, moderate ventilation is realized, the sanitation and disinfection of the animal house are regularly cleaned, and the animal house has the protection effect on the respiratory tract of the animals when entering and exiting completely. B. The reasonable use of the vaccine and the medication: the immunity and the medication can be used for assisting in enhancing the respiratory tract mucosa immunity of the body. Antibacterial drugs are reasonably and properly selected to enhance the respiratory tract immunity of the livestock; C. the use of the feed additive: the feed additives such as traditional Chinese medicines, probiotics, vitamins and the like can promote the development of livestock mucosa, promote the repair of damaged mucosa and improve the immunologic function of respiratory tract.
The invention improves respiratory tract mucosa immunity by two modes, additive A and additive A comprise: the compound fermented traditional Chinese medicine, vitamin A, vitamin C and vitamin E are used as a mixing additive, and the compound fermented traditional Chinese medicine is used for a long time from the animal body to enhance the immunity of respiratory mucosa. The raw materials of the additive B comprise: the bacillus subtilis, the bacillus licheniformis, the lactobacillus plantarum and the enterococcus faecalis are spray preparations, and can reduce the number of total aerobic bacteria, escherichia coli and staphylococcus aureus in the environment and improve the environment of a chicken coop from the beginning of the peripheral environment.
Chinese patent gazette CN108740340A discloses an animal feed for preventing animal respiratory diseases, which is characterized in that a traditional Chinese medicine additive is added into the feed, the traditional Chinese medicine additive comprises folium isatidis, liquorice, rheum officinale, radix scrophulariae and fructus forsythiae, the prevention and the treatment of the pig respiratory diseases are realized, the traditional Chinese medicine raw materials are singly crushed and then added, the release of active ingredients is limited, and the prevention and the treatment of the respiratory diseases are slow.
Chinese patent publication CN107952062A discloses a respiratory tract prevention and treatment medicine for animals and a preparation method thereof, the respiratory tract prevention and treatment medicine for animals comprises pidotimod, moringa oleifera, melatonin, microalgae and composite probiotics, and the invention combines the combined action of antibiotics and probiotics to treat respiratory tract diseases.
Disclosure of Invention
The invention overcomes the defects in the prior art and provides a composition for enhancing the respiratory tract immunity of animals, a method and application thereof.
The purpose of the invention is realized by the following technical scheme.
A composition for enhancing animal respiratory tract immunity comprises a feed additive added into feed and a spray composite bacteria preparation sprayed on poultry houses.
Further, the mixing additive comprises the following components in parts by weight: 70-95 parts of compound fermentation traditional Chinese medicine, 0.2-0.5 part of vitamin A, 4-8 parts of vitamin C and 1-2 parts of vitamin E.
Further, the compound fermentation traditional Chinese medicine comprises the following traditional Chinese medicine raw materials in proportion: mulberry leaf: mint: bitter apricot seeds: honeysuckle flower: astragalus membranaceus 2:1:1:1: 1.
Further, the addition ratio of each raw material component in the spray composite bacteria preparation is bacillus subtilis: b, bacillus licheniformis: lactobacillus plantarum: enterococcus faecalis 1:1:1: 1.
A method for preparing a composition for enhancing respiratory immunity in an animal comprising the steps of:
s1: preparing a compound fermentation traditional Chinese medicine: mixing the traditional Chinese medicines according to a compound traditional Chinese medicine formula, inoculating a compound strain according to 0.5% of the mass of the compound traditional Chinese medicines, and then fermenting; fermenting at about 25 deg.C for one week, turning over for 2-3 times per day, fermenting, and vacuum drying under reduced pressure. After the fermentation is finished, the fermentation faint scent exists, the water content reaches 39 percent, the PH value is less than or equal to 4.0, and the total viable count is more than or equal to 2 x 108CFU/g。
S2: preparing a material mixing additive, namely mixing the compound fermented traditional Chinese medicine obtained in the S1 with vitamins;
s3: preparing a spray composite bacterium preparation: weighing Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum and enterococcus faecalis according to a certain proportion, dissolving in water, and stirring to obtain suspension.
Further, in the S1, the compound traditional Chinese medicine formula contains the following traditional Chinese medicine raw materials in an addition ratio: mulberry leaf: mint: bitter apricot seeds: honeysuckle flower: astragalus membranaceus 2:1:1:1: 1.
Further, the preferred composite strains in the fermentation in S1 are a mixture of bacillus subtilis, saccharomycetes, clostridium butyricum and enterococcus faecalis, and the total viable count is more than or equal to 2 x 1010
Further, the step of preparing the compound traditional Chinese medicine in the step S1 is as follows:
firstly, crushing and sieving each traditional Chinese medicine raw material of the compound traditional Chinese medicine formula by a 30-mesh sieve;
step two, heating the mixed materials in the step one for 45 minutes at the temperature of 121 ℃, sterilizing by using steam, and controlling the water content after sterilization to be 40-60%;
the addition proportion of each raw material component in the spray composite bacteria preparation in the S3 is that the raw material components are bacillus subtilis: b, bacillus licheniformis: lactobacillus plantarum: enterococcus faecalis 1:1:1: 1.
The feed additive for enhancing the respiratory tract immunity of animals is applied to feed additives and bacteriostasis of meat poultry houses.
The invention has the beneficial effects that:
the solid fermentation composite traditional Chinese medicine has the advantages of rich resources, greenness, no pollution, outstanding biological functions and resistance reduction, and is widely used in animal breeding at present. The invention mainly combines the particularity of the respiratory tract immunity of animals, selects and compounds a plurality of traditional Chinese medicines for improving the respiratory tract mucosa. Licorice root: invigorating spleen and replenishing qi, clearing heat and detoxicating, eliminating phlegm and relieving cough, and has obvious prevention and treatment effect on respiratory diseases; mulberry leaf: the fresh mulberry leaf decoction has strong antibacterial effect on staphylococcus aureus, diphtheria bacillus and anthrax bacillus, and also has certain antibacterial effect on escherichia coli, typhoid bacillus, dysentery bacillus and pseudomonas aeruginosa. Mint: relieving sore throat, dispelling pathogenic wind and heat, refreshing mind, dispersing stagnated liver qi, and activating qi-flowing. Bitter apricot seeds: it is indicated for cough, dyspnea, fullness in chest and profuse phlegm because it can direct qi downward, relieve cough and dyspnea. Honeysuckle flower: clearing away heat and toxic material, resisting bacteria, diminishing inflammation, and enhancing immunity. Astragalus root: has antiviral, anti-stress, and antioxidant effects, and can be used as immunostimulant and regulator.
The compound vitamin has important effect on respiratory tract mucosa immunity. The vitamin A promotes the hyperplasia of skin and mucosa, stabilizes epithelial cell membranes, participates in the immune regulation of respiratory tract mucosa, can relieve the stress effect of lipopolysaccharide and ammonia gas on respiratory tracts, and improves the immune function of the respiratory tracts. Vitamin C improves the bactericidal and antiviral abilities of the body, forms collagen, and maintains the integrity and firmness of the skin and respiratory mucosa; the vitamin E antioxidant can enhance the detoxification capability of the liver and improve the immunity of the organism.
The raw materials in the additive B comprise a composite probiotic preparation, the bacillus subtilis and the bacillus licheniformis are bacillus lactic acid bacteria, the tolerance is strong, and the bacillus subtilis and the bacillus licheniformis can inhibit the growth of microorganisms in the environment and reduce pathogenic microorganisms carried by the air in a house when sprayed in the air. The lactobacillus plantarum and the enterococcus faecalis belong to lactic acid bacteria, have strong acid production capacity, can inhibit the field planting of escherichia coli, adjust the diversity of animal respiratory tract flora and improve the immunity of respiratory tracts. The composite probiotics enter the animal body, and can secrete protease, cellulase and the like in the animal metabolism process, promote the digestion and absorption of nutrient substances, reduce the discharge of harmful gases and improve the livestock and poultry breeding environment.
Compared with the prior art, the feed additive has the beneficial effects that ① composite feed additives are divided into a material mixing preparation and a spraying preparation, the respiratory tract immunity of animals is comprehensively improved from inside to outside, ② traditional Chinese medicine formula is treated in a probiotic fermentation mode to catalyze the release of active ingredients of traditional Chinese medicines and enhance the bioactivity of the active ingredients, ③ additive B adopts a novel spraying preparation mode to fully combine with a respiratory tract mucosa vulnerability mechanism and effectively enhance the respiratory tract mucosa immunity, ④ composite vitamin is added to enable multiple vitamins to act synergistically and better protect the respiratory tract mucosa, ⑤ uses a green additive to avoid the problems of antibiotic resistance and the like, improve the health of animals and human beings, ⑥ improves the animal welfare and increases the animal breeding income.
Detailed Description
The technical solution of the present invention is further illustrated by the following specific examples.
Example 1
A feed additive for enhancing animal respiratory tract immunity comprises two parts, the first part is a mixing additive, which is additive A; the second part is a spray composite bacteria preparation which is an additive B. The raw materials of the additive A comprise: 95 parts of compound fermentation traditional Chinese medicine, 0.25 part of vitamin A, 4 parts of vitamin C and 1 parts of vitamin E. Wherein the compound fermentation traditional Chinese medicine comprises: licorice root: mulberry leaf: mint: bitter apricot seeds: honeysuckle flower: astragalus membranaceus 2:1:1:1: 1. The additive B is a compound bacterium preparation and comprises the following raw materials: bacillus subtilis (1500 billion CFU/g): bacillus licheniformis (1500 hundred million CFU/g): lactobacillus plantarum (1500 hundred million CFU/g): enterococcus faecalis (1500 hundred million CFU/g) is 1:1:1:1, and the spray concentration is 2mL/m3
The effect of the feed additive for enhancing the respiratory tract immunity of animals on improving the environment of the meat poultry house is verified. The applicant carries out tests in a certain broiler farm in Shandong, 4 colony houses with consistent ventilation, illumination and feeding areas are randomly selected for testing, 2 colony houses are carried out with spraying tests, 20g of bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and enterococcus faecalis are weighed according to the proportion and dissolved in 20kg of water, and the mixture is fully stirred to be in a suspension state. Spraying once in the morning and evening every day. The test period is 42 days, and the test time is 5 months and 20 days in 2019-7 months and 1 day in 2019.
The raising management is to operate the chickens according to the normal specification of a farm, immunize according to the conventional program, perform the eye drop and nose drop immunizations of the newcastle disease and the infectious bronchitis bigeminy vaccine at the age of 7 days, and perform the drinking water immunizations of the newcastle disease at the age of 21 days. Air collectors are adopted to collect colony house gas every week, the point selection positions are 3 points in the front, middle and back, 9 points in total, each point is collected for 3 times and is parallel, and the same position is fixed in each time of collection. The total aerobe, the colibacillus and the staphylococcus aureus respectively take nutrient agar culture medium, eosin methylene blue culture medium and mannitol high-salt culture medium as sampling media. The sampling flow rate of the sampler is set to be 28.3L/min, the collection time of total aerobic bacteria and staphylococcus aureus is 2-3min, and the collection time of escherichia coli is 2-4 min. And (3) the fan is turned off during sampling, the sample is immediately placed into a constant-temperature incubator for culture after being collected, the total aerobic bacteria and the escherichia coli are cultured for 18-24 hours, the staphylococcus aureus is cultured for 24-36 hours, and then identification and counting are carried out.
The test data were statistically analyzed using SPSS12.0 statistical software, and the results were expressed as "mean. + -. standard deviation" by single factor variance comparison. The test results are shown in the following tables 1 and 2:
TABLE 1 Effect of additives on the air microbial content of henhouse (lgCFU/m)3)
Figure BDA0002348975400000051
Remarking: the same column is annotated with different letters indicating significant difference (p < 0.05).
The test result shows that with the increase of the feeding days, the air quality of the colony house is reduced, the number of harmful microorganisms is increased, and the total number of aerobes, escherichia coli and staphylococcus aureus in the test group is obviously lower than that in the control group (p is less than 0.05) at the age of 21 days. At the age of 42 days, the total aerobes, Escherichia coli and Staphylococcus aureus of the test group are significantly lower than those of the control group (p <0.05)
TABLE 2 Effect of additives on microbial content of broiler excrements in different housing houses (lgCFU/m)3)
Figure BDA0002348975400000052
Remarking: the same column is annotated with different letters indicating significant difference (p < 0.05).
As can be seen from the table, the microbial number of the excrement of the broilers increases with the increase of the feeding days, and the total aerobes, escherichia coli and staphylococcus aureus in the test group are obviously lower than those in the control group (p <0.05) at the age of 21 days. At 42 days of age, the total aerobes, E.coli, S.aureus in the test group were significantly lower than the control group (p < 0.05).
The above examples show that the additive of the invention can obviously reduce the contents of microorganisms such as total aerobes, escherichia coli, staphylococcus aureus and the like in the air and excrement of a colony house, purify the breeding environment of animals and reduce the incidence rate of respiratory diseases.
Example 2
A feed additive for enhancing animal respiratory tract immunity comprises two parts, the first part is a mixing additive, which is additive A; the second part is a spray composite bacteria preparation which is an additive B. The raw materials of the additive A comprise: 92 parts of compound fermentation traditional Chinese medicine, 0.35 part of vitamin A, 6 parts of vitamin C and 2 parts of vitamin E. Wherein the compound fermentation traditional Chinese medicine comprises: licorice root: mulberry leaf: mint: bitter apricot seeds: honeysuckle flower: astragalus membranaceus 2:1:1:1: 1. The additive B is a compound bacterium preparation and comprises the following raw materials: bacillus subtilis (1500 billion CFU/g): bacillus licheniformis (1500 hundred million CFU/g): lactobacillus plantarum (1500 hundred million CFU/g): enterococcus faecalis (1500 hundred million CFU/g) is 1:1:1:1, and the spray concentration is 2mL/m3
The influence of the feed additive for enhancing the respiratory tract immunity of animals on the respiratory tract mucosal immunity of the broiler is verified. The test is carried out in a broiler farm in Shaanxi province, the broiler variety is AA broiler, two broiler breeds are randomly selected for testing, one group is a control group, the other group is a test group, and the additive is added into the test group. The broiler chicken is stored in a farm of 3000 feathers.
The test method comprises the following steps: at 21 days of age, 10 samples were randomly selected for each treatment. Collecting blood of wing vein, centrifuging at 4 deg.C for 10min at 3000r/min, separating plasma, freezing at 20 deg.C for preservation, and killing after collecting blood of wing vein; fixing trachea 10% formalin with length of about lcm, dehydrating, transparentizing, soaking in wax, embedding, and slicing; after tracheotomy, placing an indwelling needle, irrigating with lml PBS, repeating for 3 times, gently massaging the chest of the animal for 20-30s, recovering alveolar lavage fluid mucoprotein with recovery rate of 80-90% and 3000r/min, centrifuging for 5rain, and storing the supernatant in a refrigerator at 20 ℃ for determination. Subpackaging trachea and lung, and quick freezing at-80 deg.C for storage.
The test results are shown in tables 3, 4 and 5 below:
TABLE 3 Effect of additives on broiler serum IgA
Group of IgA(ng/mL)
Control group 820.76±25.18b
Test group 916.79±23.96a
Remarking: the same column is annotated with different letters indicating significant difference (p < 0.05).
Test results show that the additive can improve the serum IgA content of the broiler chicken and enhance the respiratory tract immunity of the broiler chicken.
TABLE 4 Effect of additives on alveolar lavage fluid mucin concentration
Group of Pulmonary alveolar lavage fluid mucin (ug/mL)
Control group 612.35±35.73b
Test group 873.77±25.18a
Remarking: the same column is annotated with different letters indicating significant difference (p < 0.05).
The test result shows that the additive can increase the concentration of the mucoprotein of the alveolar lavage fluid and promote the growth of respiratory mucosa.
TABLE 5 Effect on the content of IgA in alveolar lavage fluid
Group of IgA (ng/mL) alveolar lavage fluid
Control group 601.46±36.12b
Test group 769.46±25.54a
Remarking: the same column is annotated with different letters indicating significant difference (p < 0.05).
Test results show that the additive can increase the content of IgA of alveolar lavage fluid, enhance the immunity of respiratory tracts and protect the respiratory tracts from stress and disease.
The two embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.

Claims (10)

1. A composition for enhancing respiratory immunity in an animal, comprising: consists of a mixing additive added into feed and a spray composite bacteria preparation sprayed on poultry houses.
2. The composition for enhancing respiratory immunity in animals according to claim 1, wherein: the material mixing additive comprises the following components in parts by weight: 70-95 parts of compound fermentation traditional Chinese medicine, 0.2-0.5 part of vitamin A, 4-8 parts of vitamin C and 1-2 parts of vitamin E.
3. The composition for enhancing respiratory immunity in animals according to claim 2, wherein: the compound fermentation traditional Chinese medicine comprises the following traditional Chinese medicine raw materials in an adding ratio of liquorice: mulberry leaf: mint: bitter apricot seeds: honeysuckle flower: astragalus membranaceus 2:1:1:1: 1.
4. The composition for enhancing respiratory immunity in animals according to claim 1, wherein: the spraying composite bacterium preparation comprises the following raw material components in proportion: b, bacillus licheniformis: lactobacillus plantarum: enterococcus faecalis 1:1:1: 1.
5. A method of preparing a composition for enhancing respiratory immunity in an animal according to any one of claims 1 to 4, wherein: the method comprises the following steps:
s1: preparing a compound fermentation traditional Chinese medicine: mixing Chinese medicinal materials according to compound Chinese medicinal composition, inoculating compound strain according to 0.5% of the weight of the Chinese medicinal materials, fermenting until the fermented product has faint scent, water content of 39%, pH of 4.0 or less, and total viable count of 2 x 10 or more8CFU/g;
S2: preparing a material mixing additive, namely mixing the compound fermented traditional Chinese medicine obtained in the S1 with vitamins;
s3: preparing a spray composite bacterium preparation: weighing Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum and enterococcus faecalis according to a certain proportion, dissolving in water, and stirring to obtain suspension.
6. The method of claim 5, wherein: the compound traditional Chinese medicine formula in the S1 is prepared from the following traditional Chinese medicine raw materials in proportion: mulberry leaf: mint: bitter apricot seeds: honeysuckle flower: astragalus membranaceus 2:1:1:1: 1.
7. The method of claim 5, wherein: the composite strains adopted in the fermentation in the S1 are a mixture of bacillus subtilis, saccharomycetes, clostridium butyricum and enterococcus faecalis, and the total viable count is more than or equal to 2 x 1010
8. The method of claim 5, wherein: the step of preparing the compound traditional Chinese medicine in the step S1 is as follows:
firstly, crushing and sieving each traditional Chinese medicine raw material of the compound traditional Chinese medicine formula by a 30-mesh sieve;
and secondly, heating the mixed materials in the first step at the temperature of 121 ℃ for 45 minutes, and sterilizing by using steam, wherein the water content after sterilization is controlled to be 40-60%.
9. The method of claim 5, wherein: the addition proportion of each raw material component in the spray composite bacteria preparation in the S3 is that the raw material components are bacillus subtilis: b, bacillus licheniformis: lactobacillus plantarum: enterococcus faecalis 1:1:1: 1.
10. Use of the feed additive for enhancing respiratory tract immunity of animals according to any one of claims 1 to 4 in feed additives and bacteriostasis of meat poultry houses.
CN201911407185.7A 2019-12-31 2019-12-31 Composition for enhancing respiratory tract immunity of animals, method and application thereof Pending CN111034855A (en)

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Application publication date: 20200421