CN109221737B - Antibiotic-free fermented feed for weaned piglets and preparation method and application thereof - Google Patents
Antibiotic-free fermented feed for weaned piglets and preparation method and application thereof Download PDFInfo
- Publication number
- CN109221737B CN109221737B CN201811295681.3A CN201811295681A CN109221737B CN 109221737 B CN109221737 B CN 109221737B CN 201811295681 A CN201811295681 A CN 201811295681A CN 109221737 B CN109221737 B CN 109221737B
- Authority
- CN
- China
- Prior art keywords
- parts
- powder
- tea seed
- seed meal
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 74
- 235000012054 meals Nutrition 0.000 claims abstract description 58
- 235000019225 fermented tea Nutrition 0.000 claims abstract description 32
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 21
- 239000012138 yeast extract Substances 0.000 claims abstract description 21
- 239000011782 vitamin Substances 0.000 claims abstract description 19
- 235000013343 vitamin Nutrition 0.000 claims abstract description 19
- 229940088594 vitamin Drugs 0.000 claims abstract description 19
- 229930003231 vitamin Natural products 0.000 claims abstract description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 17
- 240000008892 Helianthus tuberosus Species 0.000 claims abstract description 16
- 235000003230 Helianthus tuberosus Nutrition 0.000 claims abstract description 16
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 15
- 240000008042 Zea mays Species 0.000 claims abstract description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 15
- 235000005822 corn Nutrition 0.000 claims abstract description 15
- 239000004467 fishmeal Substances 0.000 claims abstract description 15
- 241000237502 Ostreidae Species 0.000 claims abstract description 14
- 235000013312 flour Nutrition 0.000 claims abstract description 14
- 235000020636 oyster Nutrition 0.000 claims abstract description 14
- 239000004575 stone Substances 0.000 claims abstract description 14
- 235000008939 whole milk Nutrition 0.000 claims abstract description 14
- 241000282887 Suidae Species 0.000 claims abstract description 8
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 6
- 235000013616 tea Nutrition 0.000 claims description 31
- 241001122767 Theaceae Species 0.000 claims description 30
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 27
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 27
- 238000002156 mixing Methods 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 14
- 210000005253 yeast cell Anatomy 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- -1 compound vitamin Chemical class 0.000 claims description 12
- 230000000813 microbial effect Effects 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 9
- 150000001875 compounds Chemical class 0.000 claims description 9
- 108090000145 Bacillolysin Proteins 0.000 claims description 8
- 102000004882 Lipase Human genes 0.000 claims description 8
- 108090001060 Lipase Proteins 0.000 claims description 8
- 239000004367 Lipase Substances 0.000 claims description 8
- 102000035092 Neutral proteases Human genes 0.000 claims description 8
- 108091005507 Neutral proteases Proteins 0.000 claims description 8
- 241001052560 Thallis Species 0.000 claims description 8
- 235000019421 lipase Nutrition 0.000 claims description 8
- 241000194032 Enterococcus faecalis Species 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 7
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 241000193749 Bacillus coagulans Species 0.000 claims description 5
- 241000186018 Bifidobacterium adolescentis Species 0.000 claims description 5
- 229940054340 bacillus coagulans Drugs 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 3
- 229930195725 Mannitol Natural products 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000000594 mannitol Substances 0.000 claims description 3
- 235000010355 mannitol Nutrition 0.000 claims description 3
- 239000008213 purified water Substances 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 16
- 206010030113 Oedema Diseases 0.000 abstract description 11
- 206010012735 Diarrhoea Diseases 0.000 abstract description 7
- 230000004083 survival effect Effects 0.000 abstract description 7
- 230000029087 digestion Effects 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 230000006870 function Effects 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 230000002354 daily effect Effects 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000036039 immunity Effects 0.000 description 6
- 235000016709 nutrition Nutrition 0.000 description 6
- 239000003053 toxin Substances 0.000 description 6
- 231100000765 toxin Toxicity 0.000 description 6
- 108700012359 toxins Proteins 0.000 description 6
- 239000003242 anti bacterial agent Substances 0.000 description 5
- 229940088710 antibiotic agent Drugs 0.000 description 5
- 244000000010 microbial pathogen Species 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 239000006041 probiotic Substances 0.000 description 5
- 235000018291 probiotics Nutrition 0.000 description 5
- 235000019750 Crude protein Nutrition 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 3
- 229930182490 saponin Natural products 0.000 description 3
- 150000007949 saponins Chemical class 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000005176 gastrointestinal motility Effects 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- 206010003591 Ataxia Diseases 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000227166 Harrimanella hypnoides Species 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 235000019742 Vitamins premix Nutrition 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229960003067 cystine Drugs 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000000744 eyelid Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 208000010726 hind limb paralysis Diseases 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000008621 organismal health Effects 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 239000008262 pumice Substances 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229940075887 saccharomyces cerevisiae extract Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 239000011647 vitamin D3 Substances 0.000 description 1
- 239000011652 vitamin K3 Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Inorganic Chemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses an antibiotic-free fermented feed for weaned pigs, a preparation method and application thereof, and belongs to the technical field of feed fermentation. The invention relates to an antibiotic-free fermented feed for weaned pigs, which comprises the following components in parts by weight: 40-50 parts of corn flour, 15-25 parts of fermented tea seed meal, 15-25 parts of whole milk powder, 2-5 parts of jerusalem artichoke powder, 2-5 parts of yeast extract, 2-5 parts of fish meal, 0.5-1.5 parts of oyster powder, 0.2-1 part of water-supply stone powder and 0.05-0.1 part of vitamin complex. The antibiotic-free fermented feed for the weaned piglets can improve the growth performance of the piglets, reduce the feed-weight ratio, increase the survival rate, enhance the digestion and absorption functions of the piglets, reduce the diarrhea rate, improve the survival rate and reduce the occurrence of edema diseases.
Description
Technical Field
The invention belongs to the technical field of feed fermentation, and particularly relates to an antibiotic-free fermented feed for weaned pigs, a preparation method and application thereof.
Background
Edema disease of piglets is an acute, highly lethal infectious disease of piglets caused by hemolytic escherichia coli. The disease mainly occurs to piglets weaned for 5-15 days, the sick piglets are poor in mental status, poor in appetite or abolished, like drinking water, eyelid and head edema, convulsion, cold tremor, ataxia and hind limb paralysis, the disease course is fast, the death rate is over 90 percent, the development of the pig industry is severely restricted, and huge economic loss is caused to a farm.
Weaning stress, nutritional stress and environmental factors are the main causes of the disease. The main solutions at present are: 1) a weaning mode of removing mother and leaving baby is adopted, so that the stress response caused by environmental change is reduced; 2) the feed is added with multiple vitamins, trace elements and amino acids to improve immunity; 3) antibiotic therapy; 4) treating with Chinese medicinal materials. The addition of nutrient substances can prevent edema diseases to a certain extent, but the edema diseases cannot be completely prevented and treated, and the adjustment of nutrient components can be used as an auxiliary prevention and treatment means; the use of antibiotics can achieve satisfactory results in a short period, and pathogenic microorganisms can generate drug resistance to the antibiotics in the past, so that the antibiotics do not accord with sustainable development strategies; the traditional Chinese medicine components are firstly discovered to be used for treating human diseases, the difference between animal and human body structures is large, the balance of yin and yang and transformation of qi and body fluid of an animal organism are not disclosed, the blindly used traditional Chinese medicine components can possibly obtain better effect but can also disturb the balance of the animal organism, in addition, the special smell of the traditional Chinese medicine reduces the food calling performance, and the food consumption of weaned piglets is reduced.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a green and safe piglet feed which can improve the immunity of weaned piglets, reduce diarrhea of piglets and prevent edema diseases without adding antibiotics, and the invention also aims to provide a preparation method of the feed.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the nonreactive fermented feed for the weaned piglets comprises the following components in parts by weight:
40-50 parts of corn flour, 15-25 parts of fermented tea seed meal, 15-25 parts of whole milk powder, 2-5 parts of jerusalem artichoke powder, 2-5 parts of yeast extract, 2-5 parts of fish meal, 0.5-1.5 parts of oyster powder, 0.2-1 part of water-supply stone powder and 0.05-0.1 part of vitamin complex.
On the basis of the scheme, the antibiotic-free fermented feed for the weaned piglets comprises the following components in parts by weight:
45 parts of corn flour, 20 parts of fermented tea seed meal, 20 parts of whole milk powder, 3 parts of jerusalem artichoke powder, 3 parts of yeast extract, 3 parts of fish meal, 1 part of oyster powder, 0.5 part of water-supply stone powder and 0.08 part of compound vitamin.
On the basis of the scheme, the preparation method of the fermented tea seeds comprises the following steps:
(1) crushing tea seed meal, sieving with a 20-mesh sieve, sterilizing with high-pressure steam at 121 ℃ for 30min, and cooling to room temperature after sterilization;
(2) adjusting the water content of the sterilized tea seed meal to be 90%, and adding biological enzyme into the tea seed meal for enzymolysis;
(3) adding 1.5-2.0% of compound microbial agent into the tea seed meal powder after enzymolysis, and fermenting for 24-36 h at 32 ℃;
(4) after the fermentation is finished, adding mannitol accounting for 5 percent of the total weight into the fermentation product, uniformly mixing, and carrying out vacuum freeze drying to obtain powder, thus obtaining the fermented tea seed meal.
On the basis of the scheme, the biological enzyme enzymolysis treatment comprises the following steps:
adding lipase and neutral protease into tea seed meal powder with the water content of 90%, wherein the addition amount of the lipase is 2 u/g; the addition amount of the neutral protease is 0.5 u/g; after being uniformly mixed, the mixture is treated for 2.5 to 4 hours at the temperature of 40 ℃ and at the pH value of 7.5; treating at 50 deg.C for 30 min; after the enzymolysis is finished, the mixture is treated for 10min at 100 ℃ and cooled to room temperature.
On the basis of the scheme, the compound microbial agent is as follows: enterococcus faecalis, lactobacillus rhamnosus, bacillus coagulans and bifidobacterium adolescentis are mixed according to the mass ratio of 2: 1.5: 2: 1.
On the basis of the scheme, the yeast extract is prepared by mixing yeast cell walls and yeast contents according to the weight ratio of 1: 2.5.
On the basis of the scheme, the preparation method of the yeast cell wall comprises the following steps: centrifuging the yeast zymocyte liquid to collect wet thalli, cleaning with sterile water for 2 times, adding purified water with 2 times of the weight of the wet thalli, performing ultrasonic wall breaking treatment, incubating at 35 ℃ for 30min, centrifuging to collect yeast cell walls, and drying into powder to obtain the yeast zymocyte liquid;
the preparation method of the yeast content comprises the following steps: freeze drying the supernatant to obtain powder.
On the basis of the scheme, the yeast zymocyte liquid is mixed bacterium liquid of candida utilis and saccharomyces cerevisiae in the mass ratio of 1: 1.
On the basis of the scheme, the preparation method of the antibiotic-free fermented feed for the weaned piglets comprises the following steps:
(1) weighing Jerusalem artichoke powder, yeast extract, fish meal, oyster powder, water-supply stone powder and compound vitamin, and uniformly mixing to obtain a mixture A;
(2) uniformly mixing the whole milk powder, the fermented tea seed meal and the corn flour according to the amount to obtain a mixture B;
(3) and mixing A and B uniformly to obtain the product.
The antibiotic-free fermented feed for the weaned pigs prepared by the method is applied to improving the immunity of the weaned pigs and preventing and treating edema disease of the weaned pigs.
The water-feeding stone is purchased from the Shanghai Co Ltd of ink Teng, and is mechanically ground into powder and sieved by a sieve of 80 meshes;
the jerusalem artichoke powder is purchased from the Biotech company Limited of Feng and Hei in Xian;
the compound vitamin is a vitamin premix feed B223 for nourishing livestock and poultry, and is purchased from eight-animal science and technology Limited company in New rural areas;
the lipase is purchased from Ningxia Shensheng industry group GmbH, and has enzyme activity of 10000 u/g;
the neutral protease is purchased from Ningxia Shengshi group of industries, Inc., and the enzyme activity is 50000 u/g;
enterococcus faecalis (Enterococcus faecalis) No.: CICC 23658, China center for the preservation and management of industrial microbial strains;
the Lactobacillus rhamnosus (Lactobacillus rhamnosus) is Lactobacillus rhamnosus W27 with the preservation number of CGMCC No. 11387;
bacillus coagulans (Bacillus coagulosns) No.: CICC 21736, China center for culture Collection of Industrial microorganisms;
bifidobacterium adolescentis CICC6178, china industrial microbial strain preservation management center;
candida utilis (Candida utilis) is numbered AS2.281, Beina Chuanglian Biotech limited;
saccharomyces cerevisiae (Saccharomyces cerevisiae) with the number of ACCC 20065, China agricultural microbial strain preservation management center.
The technical scheme of the invention has the advantages that:
the antibiotic-free fermented feed for the weaned piglets can improve the growth performance of the piglets, reduce the feed-weight ratio, increase the survival rate, enhance the digestion and absorption functions of the piglets, reduce the diarrhea rate, improve the survival rate and reduce the occurrence of edema diseases.
The tea seed meal in the antibiotic-free fermented feed is prepared by microbial fermentation after enzymolysis, lipase can degrade residual oil components in the tea seed meal, neutral protease can degrade macromolecular protein components, and the tea seed meal after enzymolysis is more favorable for digestion and absorption of piglets; in addition, the tea seed meal subjected to enzymolysis has a loose structure, so that microbial fermentation is facilitated; the compound microorganism fermentation can increase the content of mycoprotein and the content of organic acid in tea seed meal, so that the fermented tea seed meal is sour and sweet and palatable, in addition, the compound microorganism produces a large amount of probiotics through fermentation and reproduction, the probiotics enter the intestinal tract of animals through food, the stability of the intestinal tract environment can be adjusted, the reproduction of pathogenic microorganisms is inhibited, in addition, the enterococcus faecalis can produce natural antibiotics, so that the health of organisms is maintained, and the growth of pathogenic escherichia coli is inhibited.
The jerusalem artichoke powder contains a large amount of starch, inulin, fructose and water-soluble dietary fiber, promotes the growth and the propagation of probiotics, increases the gastrointestinal peristalsis, and promotes the discharge of toxins and excrement; further enhancing the effect of the probiotics on inhibiting the growth of pathogenic microorganisms.
The yeast cell content contains a large amount of polypeptide, amino acid, vitamin and trace elements, the nutrition is rich, the yeast cell wall is rich in beta-glucan and mannan, and the yeast cell wall has various physiological functions of enhancing immunity, preventing and treating diseases, promoting growth, relieving stress, adsorbing mycotoxin, supplementing nutrition and the like. In general, active yeast is surrounded by a solid cell wall structure, and the contents are difficult to release. The invention uses ultrasonic wall breaking technology to completely release the yeast content, incubates at 35 ℃ and degrades the content under the action of own biological enzyme to form micromolecule active substance which is absorbed by organism. The yeast content combines with yeast cell wall components to play a role in promoting growth, improving immunity and adsorbing toxins and harmful microorganisms.
The pumice, also called absorbent stone, is fossil of calcium carbonate moss plants, has a natural porous structure and contains rich trace elements, and can be matched with yeast cell walls to adsorb toxins and harmful microorganisms together, promote gastrointestinal motility and discharge toxins.
The components in the antibiotic-free fermented feed are matched with each other and complement each other, so that the propagation of probiotics is promoted, the growth of pathogenic microorganisms is inhibited, toxins and the pathogenic microorganisms are adsorbed, the gastrointestinal motility is promoted, the toxin discharge is accelerated, the immunity is improved, the diarrhea rate is reduced, and the growth is promoted.
Detailed Description
Terms used in the present invention have generally meanings as commonly understood by one of ordinary skill in the art, unless otherwise specified.
The present invention will be described in further detail with reference to the following data in conjunction with specific examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.
Example 1
The method for fermenting the tea seed meal comprises the following steps:
(1) crushing tea seed meal, sieving with a 20-mesh sieve, sterilizing with high-pressure steam at 121 ℃ for 30min, and cooling to room temperature after sterilization;
(2) adjusting the water content of the sterilized tea seed meal to be 90%, and adding lipase and neutral protease into the tea seed meal, wherein the addition amount of the lipase is 2 u/g; the addition amount of the neutral protease is 0.5 u/g; after being uniformly mixed, the mixture is treated for 2.5 to 4 hours at the temperature of 40 ℃ and at the pH value of 7.5; treating at 50 deg.C for 30 min; after the enzymolysis is finished, processing for 10min at 100 ℃, and cooling to room temperature;
(3) adding 1.5-2.0% of compound microbial agent into the tea seed meal powder after enzymolysis, and fermenting for 24-36 h at 32-35 ℃;
the compound microbial agent is as follows: enterococcus faecalis, lactobacillus rhamnosus, bacillus coagulans and bifidobacterium adolescentis are mixed according to the mass ratio of 2: 1.5: 2: 1.
(4) After the fermentation is finished, adding mannitol accounting for 5 percent of the total weight into the fermentation product, uniformly mixing, and carrying out vacuum freeze drying to obtain powder, thus obtaining the fermented tea seed meal.
The viable count of the enterococcus faecalis is 1 multiplied by 109CFU/g；
The viable count of the lactobacillus rhamnosus is 1 multiplied by 109CFU/g；
The viable count of the bacillus coagulans is 1 multiplied by 108CFU/g；
The number of viable Bifidobacterium adolescentis is 1 × 109CFU/g。
First, the influence of fermentation on the nutrient components of tea seed meal
Test grouping
Example 1 group: tea seed meal fermented by the method of example 1;
control group 1: example 1 tea seed meal fermented directly without enzymatic hydrolysis;
control group 2: example 1 tea seed meal which was only enzymatically hydrolyzed and not microbially fermented;
blank control group: crushing the tea seed meal into powder of 20 meshes;
the contents of tea saponin, crude protein, reducing sugar, organic matters, organic acid and crude fiber in the tea seed meal of each group are measured as shown in table 1:
TABLE 1 Effect of fermentation on the nutritional content of tea seed meal (%)
| Group of | EXAMPLE 1 group | Control group 1 | Control group 2 | Blank control group |
| Tea saponin | 1.38 | 4.05 | 7.16 | 10.52 |
| Crude protein | 20.65 | 17.27 | 13.12 | 12.76 |
| Reducing sugar | 0.98 | 1.18 | 1.24 | 1.48 |
| Organic matter | 70.11 | 67.58 | 66.81 | 66.53 |
| Organic acids | 1.27 | 0.84 | 0.33 | 0.35 |
| Coarse fiber | 11.43 | 18.47 | 17.85 | 23.16 |
As can be seen from table 1, the content of tea saponin in the tea seed meal prepared by the enzymolysis and fermentation in example 1 is significantly reduced, the content of crude protein, organic matter and organic acid is significantly increased, and the content of reducing sugar and crude fiber is significantly reduced, compared with the control group and the blank control group.
Example 2
The nonreactive fermented feed for the weaned piglets comprises the following components in parts by weight:
40 parts of corn flour, 25 parts of fermented tea seed meal, 25 parts of whole milk powder, 2 parts of jerusalem artichoke powder, 2 parts of yeast extract, 2 parts of fish meal, 0.5 part of oyster powder, 0.2 part of water stone powder and 0.05 part of compound vitamin.
The fermented tea seed meal is the fermented tea seed meal prepared by the method in the embodiment 1;
the preparation method of the yeast extract comprises the following steps:
candida utilis (Candida utilis) is numbered AS2.281, Beina Chuanglian Biotech limited;
the number of the Saccharomyces cerevisiae (Saccharomyces cerevisiae) is ACCC 20065, China agricultural microbial strain preservation management center;
A. respectively inoculating candida utilis and saccharomyces cerevisiae into a liquid fermentation culture medium, culturing for 3d at the temperature of 30-35 ℃, respectively centrifugally collecting wet thalli of the candida utilis and the saccharomyces cerevisiae, and mixing according to the mass ratio of 1: 1;
the liquid fermentation culture medium comprises: 40g/L glucose, 10g/L, KH peptone2PO42g/L、MgSO4·7H2O 0.3g/L, adding water to a constant volume of 1L; sterilizing with high pressure steam at 121 deg.C for 15 min;
B. adding sterile water into the mixed wet thalli to clean for 2 times, adding purified water with the weight being 2 times that of the wet thalli, performing ultrasonic wall breaking treatment, performing ultrasonic power of 500-600W for 10-15 min, and radiating for 10 s; incubating the liquid after the ultrasonic wall breaking treatment at 35 ℃ for 30min, centrifuging and collecting the yeast cell wall, and drying into powder to obtain the yeast cell wall; and freeze-drying the supernatant into powder to obtain the yeast content.
C. Mixing the yeast cell wall and the yeast content according to the weight ratio of 1: 2.5 to obtain the yeast extract.
The preparation method of the antibiotic-free fermented feed for the weaned piglets comprises the following steps:
(1) weighing Jerusalem artichoke powder, yeast extract, fish meal, oyster powder, water-supply stone powder and compound vitamin, and uniformly mixing to obtain a mixture A;
(2) uniformly mixing the whole milk powder, the fermented tea seed meal and the corn flour according to the amount to obtain a mixture B;
(3) and mixing A and B uniformly to obtain the product.
Example 3
The nonreactive fermented feed for the weaned piglets comprises the following components in parts by weight:
45 parts of corn flour, 20 parts of fermented tea seed meal, 20 parts of whole milk powder, 3 parts of jerusalem artichoke powder, 3 parts of yeast extract, 3 parts of fish meal, 1 part of oyster powder, 0.5 part of water-supply stone powder and 0.08 part of compound vitamin.
The fermented tea seed meal is the fermented tea seed meal prepared by the method in the embodiment 1;
the yeast extract was the same as in example 2;
the preparation method of the antibiotic-free fermented feed for weaned piglets is the same as that of the example 2.
Example 4
The nonreactive fermented feed for the weaned piglets comprises the following components in parts by weight:
50 parts of corn flour, 15 parts of fermented tea seed meal, 15 parts of whole milk powder, 5 parts of jerusalem artichoke powder, 5 parts of yeast extract, 5 parts of fish meal, 1.5 parts of oyster powder, 1 part of ground limestone and 0.1 part of compound vitamin.
The fermented tea seed meal is the fermented tea seed meal prepared by the method in the embodiment 1;
the yeast extract was the same as in example 2;
the preparation method of the antibiotic-free fermented feed for weaned piglets is the same as that of the example 2.
Comparative example 1
The nonreactive fermented feed for the weaned piglets comprises the following components in parts by weight:
45 parts of corn flour, 20 parts of fermented tea seed meal, 20 parts of whole milk powder, 3 parts of jerusalem artichoke powder, 3 parts of yeast extract, 3 parts of fish meal, 1 part of oyster powder and 0.08 part of compound vitamin.
The fermented tea seed meal is the fermented tea seed meal prepared by the method in the embodiment 1;
the yeast extract was the same as in example 2;
the preparation method of the antibiotic-free fermented feed for weaned piglets is the same as that of the example 2.
Comparative example 2
The nonreactive fermented feed for the weaned piglets comprises the following components in parts by weight:
45 parts of corn flour, 20 parts of fermented tea seed meal, 20 parts of whole milk powder, 3 parts of jerusalem artichoke powder, 3 parts of yeast extract, 3 parts of fish meal, 1 part of oyster powder, 0.5 part of water-supply stone powder and 0.08 part of compound vitamin.
The fermented tea seed meal is the fermented tea seed meal prepared by the method in the embodiment 1;
the yeast extract is the single saccharomyces cerevisiae extract in example 2;
the preparation method of the antibiotic-free fermented feed for weaned piglets is the same as that of the example 2.
Comparative example 3
The nonreactive fermented feed for the weaned piglets comprises the following components in parts by weight:
45 parts of corn flour, 20 parts of fermented tea seed meal, 20 parts of whole milk powder, 3 parts of jerusalem artichoke powder, 3 parts of yeast, 3 parts of fish meal, 1 part of oyster powder, 0.5 part of water-supply stone powder and 0.08 part of compound vitamin.
The fermented tea seed meal is the fermented tea seed meal prepared by the method in the embodiment 1;
the yeast is yeast powder formed by mixing and drying wet thalli of saccharomyces cerevisiae and candida utilis according to the mass ratio of 1: 1;
the preparation method of the antibiotic-free fermented feed for weaned piglets is the same as that of the example 2.
Secondly, the influence of the antibiotic-free fermented feed on the growth performance of the weaned piglets
Test grouping
Examples 2-4 and comparative examples 1-3;
test method
140 healthy 28-day-old and close-weight Du x Long x big weaned piglets were selected and randomly divided into 7 groups of 4 replicates each, with 5 replicates each, wherein: 1-3 groups of the antibiotic-free fermented feed are fed with the antibiotic-free fermented feed of examples 1-3 respectively, and 4-6 groups of the antibiotic-free fermented feed of comparative examples 1-3 are fed with the antibiotic-free fermented feed respectively; group 7 was fed regular weaned piglet diet (table 2) and scored as a blank control.
And (3) during the test period of 30d, weighing the weight of the pig at the beginning and the end of the test respectively, and performing blind test in the whole test process. Raising in a fence mode in the whole test period, and feeding for 3-4 times a day. Cleaning the feed tank at 07:00 a.m. every day, counting feed intake, freely drinking water, and cleaning the barn for 1 time respectively in the morning and afternoon. Temperature and humidity were recorded daily. The daily management and immunization program are carried out according to the conventional method.
TABLE 2 basic daily ration raw material composition and nutritional ingredients
| Item | Content (wt.) |
| Raw material composition% | |
| Puffed corn | 38.5 |
| Broken rice | 13.7 |
| Puffed soybean | 10.0 |
| Soybean meal | 10.8 |
| Fish meal | 8.3 |
| Whey powder | 12.7 |
| Sucrose | 2.0 |
| Premix compound① | 4.0 |
| Nutritional index | |
| Dry matter% | 89.11 |
| Digestion energy, MJ/kg | 13.92 |
| Crude protein% | 19.66 |
| Calcium content% | 0.80 |
| Total phosphorus% | 0.69 |
| Effective phosphorus% | 0.51 |
| Lysine% | 1.46 |
| Methionine + cystine% | 0.72 |
Note:①the premix is provided for each kilogram of daily ration: vitamin A250000IU, vitamin D31600IU, vitamin E48.3 mg, vitamin K346.6mg of vitamin B67.5mg, 42mg nicotinic acid, 10mg pantothenic acid, 150mg iron, 160mg zinc, 10mg copper and 70mg manganese.
The results of measuring the feed-to-weight ratio, the piglet diarrhea rate, the piglet survival rate and the edema disease rate are shown in table 3:
TABLE 3 Effect of antibiotic-free fermented feed on growth Performance of weaned piglets
| Group of | Average daily gain g | Material to weight ratio | The rate of diarrhea% | Survival rate% | The disease rate of edema disease% |
| Example 2 | 411.85 | 1.62 | 1.04 | 95 | 5 |
| Example 3 | 417.63 | 1.60 | 0.62 | 100 | 0 |
| Example 4 | 413.57 | 1.62 | 0.98 | 95 | 0 |
| Comparative example 1 | 410.76 | 1.64 | 1.47 | 95 | 5 |
| Comparative example 2 | 402.35 | 1.65 | 1.94 | 95 | 5 |
| Comparative example 3 | 394.71 | 1.66 | 3.71 | 90 | 10 |
| Blank control group | 347.66 | 1.70 | 6.45 | 80 | 20 |
As can be seen from table 3, the average daily gain of piglets in examples 2 to 4 is significantly higher than that of other groups, the feed-weight ratio, diarrhea rate and edema disease rate are significantly lower than those of other groups, and the survival rate is also significantly higher than those of other groups.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (3)
1. The antibiotic-free fermented feed for weaned piglets is characterized by comprising the following components in parts by weight: the composition comprises the following components in parts by weight:
40-50 parts of corn flour, 15-25 parts of fermented tea seed meal, 15-25 parts of whole milk powder, 2-5 parts of jerusalem artichoke powder, 2-5 parts of yeast extract, 2-5 parts of fish meal, 0.5-1.5 parts of oyster powder, 0.2-1 part of water-supply stone powder and 0.05-0.1 part of vitamin complex;
the yeast extract is prepared by mixing yeast cell walls and yeast contents according to the weight ratio of 1: 2.5; the preparation method of the yeast cell wall comprises the following steps: centrifuging the yeast zymocyte liquid to collect wet thalli, cleaning with sterile water for 2 times, adding purified water with 2 times of the weight of the wet thalli, performing ultrasonic wall breaking treatment, incubating at 35 ℃ for 30min, centrifuging to collect yeast cell walls, and drying into powder to obtain the yeast zymocyte liquid; the preparation method of the yeast content comprises the following steps: freeze drying the supernatant to obtain powder; the yeast zymocyte liquid is a mixed liquid of candida utilis and saccharomyces cerevisiae with the mass ratio of 1: 1;
the preparation method of the fermented tea seeds comprises the following steps:
(1) crushing tea seed meal, sieving with a 20-mesh sieve, sterilizing with high-pressure steam at 121 ℃ for 30min, and cooling to room temperature after sterilization;
(2) adjusting the water content of the sterilized tea seed meal to be 90%, and adding biological enzyme into the tea seed meal for enzymolysis;
(3) adding 1.5-2.0% of compound microbial agent into the tea seed meal powder after enzymolysis, and fermenting for 24-36 h at 32-35 ℃;
(4) after the fermentation is finished, adding mannitol accounting for 5 percent of the total weight into the fermentation product, uniformly mixing, and carrying out vacuum freeze drying to obtain powder, thus obtaining fermented tea seed meal;
the biological enzyme enzymolysis treatment comprises the following steps:
adding lipase and neutral protease into tea seed meal powder with the water content of 90%, wherein the addition amount of the lipase is 2 u/g; the addition amount of the neutral protease is 0.5 u/g; after being uniformly mixed, the mixture is treated for 2.5 to 4 hours at the temperature of 40 ℃ and at the pH value of 7.5; treating at 50 deg.C for 30 min; after the enzymolysis is finished, processing for 10min at 100 ℃, and cooling to room temperature;
the compound microbial agent is as follows: enterococcus faecalis, lactobacillus rhamnosus, bacillus coagulans and bifidobacterium adolescentis are mixed according to the mass ratio of 2: 1.5: 2: 1.
2. The antibiotic-free fermented feed for weaned pigs according to claim 1, characterized in that: the composition comprises the following components in parts by weight:
45 parts of corn flour, 20 parts of fermented tea seed meal, 20 parts of whole milk powder, 3 parts of jerusalem artichoke powder, 3 parts of yeast extract, 3 parts of fish meal, 1 part of oyster powder, 0.5 part of water-supply stone powder and 0.08 part of compound vitamin.
3. The method for producing the antibiotic-free fermented feed for weaned pigs according to claim 1 or 2, characterized in that: the method comprises the following steps:
(1) weighing Jerusalem artichoke powder, yeast extract, fish meal, oyster powder, water-supply stone powder and compound vitamin, and uniformly mixing to obtain a mixture A;
(2) uniformly mixing the whole milk powder, the fermented tea seed meal and the corn flour according to the amount to obtain a mixture B;
(3) and mixing A and B uniformly to obtain the product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811295681.3A CN109221737B (en) | 2018-11-01 | 2018-11-01 | Antibiotic-free fermented feed for weaned piglets and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811295681.3A CN109221737B (en) | 2018-11-01 | 2018-11-01 | Antibiotic-free fermented feed for weaned piglets and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109221737A CN109221737A (en) | 2019-01-18 |
| CN109221737B true CN109221737B (en) | 2022-03-25 |
Family
ID=65080418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811295681.3A Active CN109221737B (en) | 2018-11-01 | 2018-11-01 | Antibiotic-free fermented feed for weaned piglets and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109221737B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318764A (en) * | 2011-08-30 | 2012-01-18 | 武汉天龙饲料有限公司 | Nonreactive piggy creep feed and preparation method thereof |
| CN102870993A (en) * | 2012-10-10 | 2013-01-16 | 荆州双胞胎饲料有限公司 | Compound feed additive for effectively improving intestinal canal health of pigs |
| CN103734549A (en) * | 2014-01-27 | 2014-04-23 | 辽宁禾丰牧业股份有限公司 | Large-grain milk replacer and preparing method thereof |
| CN103981129A (en) * | 2014-05-06 | 2014-08-13 | 武汉科前动物生物制品有限责任公司 | Composite microbial preparation, and preparation method and application thereof |
| CN104000030A (en) * | 2014-04-22 | 2014-08-27 | 江苏长江桂柳生物科技集团有限公司 | Anti-stress early suckling pig creep feed |
| CN105394383A (en) * | 2015-11-12 | 2016-03-16 | 六安瑞泰养殖科技有限公司 | Calcium-supplementing type edema prevention piglet feed and preparation method thereof |
| CN107927384A (en) * | 2017-11-10 | 2018-04-20 | 江苏中煤长江生物科技有限公司 | A kind of dustless creep mixed feed for piglets of nonreactive and preparation method thereof |
-
2018
- 2018-11-01 CN CN201811295681.3A patent/CN109221737B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318764A (en) * | 2011-08-30 | 2012-01-18 | 武汉天龙饲料有限公司 | Nonreactive piggy creep feed and preparation method thereof |
| CN102870993A (en) * | 2012-10-10 | 2013-01-16 | 荆州双胞胎饲料有限公司 | Compound feed additive for effectively improving intestinal canal health of pigs |
| CN103734549A (en) * | 2014-01-27 | 2014-04-23 | 辽宁禾丰牧业股份有限公司 | Large-grain milk replacer and preparing method thereof |
| CN104000030A (en) * | 2014-04-22 | 2014-08-27 | 江苏长江桂柳生物科技集团有限公司 | Anti-stress early suckling pig creep feed |
| CN103981129A (en) * | 2014-05-06 | 2014-08-13 | 武汉科前动物生物制品有限责任公司 | Composite microbial preparation, and preparation method and application thereof |
| CN105394383A (en) * | 2015-11-12 | 2016-03-16 | 六安瑞泰养殖科技有限公司 | Calcium-supplementing type edema prevention piglet feed and preparation method thereof |
| CN107927384A (en) * | 2017-11-10 | 2018-04-20 | 江苏中煤长江生物科技有限公司 | A kind of dustless creep mixed feed for piglets of nonreactive and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109221737A (en) | 2019-01-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108813098B (en) | Microbial fermentation composite antibiotic-free feed containing natural antibiotic components | |
| CN103598447B (en) | Non-antibiotic sucking pig milk replacer and preparation method | |
| CN102948621A (en) | Prebiotic peptide biological feed additive and preparation method and application thereof | |
| CN102366023A (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
| CN104206789B (en) | It is a kind of to be used to prevent mastitis for milk cows and the feed addictive of brucellosis and preparation method thereof | |
| CN101209088A (en) | Microorganism polyzyme additive agent for improving pigling growth and development | |
| CN103627656A (en) | Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans | |
| CN110946907A (en) | Traditional Chinese medicine composition for young poultry, liquid fermented traditional Chinese medicine preparation and preparation method thereof | |
| CN111227126A (en) | Anti-diarrhea growth-promoting piglet feed additive and preparation method thereof | |
| CN106974063A (en) | It is a kind of that the method that bacillus coagulans produces High efficiency protein feed is cooperateed with feeding enzyme | |
| CN108552429A (en) | A kind of feed addictive for reducing weaned piglet growth disorder | |
| CN105994941A (en) | Non-antibiotic feed prepared through microbial fermentation | |
| CN106173401A (en) | A kind of mixed bacterium asynchronous fermentation produces the preparation method of acidifying zymolysis feedstuff | |
| CN110810625A (en) | Waste mushroom stick biological fermentation feed and preparation method thereof | |
| CN109924360A (en) | One boar fermentation compound feed and preparation method thereof | |
| CN110892939A (en) | Method for producing baicalein by fermenting bacillus subtilis, feed additive prepared by fermentation and preparation method | |
| CN103263444B (en) | Micro-ecological compound preparation for preventing and treating stress diarrhoea of goat and preparation method thereof | |
| CN110574840A (en) | Traditional Chinese medicine fermented feed for preventing African swine fever and preparation method thereof | |
| CN105994937A (en) | Green organic selenium-rich feed and preparation method thereof | |
| CN112471353A (en) | Long-acting symbiotic composite liquid microecological preparation for poultry feeding and preparation method thereof | |
| CN102367416A (en) | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive | |
| CN112136965A (en) | Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof | |
| CN101999556B (en) | Special animal nutrient for beef cattle and preparation method thereof | |
| CN111616259A (en) | Production method of fermented dry feed capable of fully playing material adsorption role | |
| CN111264686A (en) | Preparation method of antibiotic-free fermented soft-particle creep feed for suckling piglets |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231114 Address after: 555000 North Nursery of Gulou Office, Gulou Street, Pingba District, Anshun City, Guizhou Province Patentee after: Guizhou Wuzi Feed Co.,Ltd. Address before: No. 700, Changcheng Road, Chengyang District, Qingdao City, Shandong Province, Shandong Patentee before: Qingdao Agricultural University |
|
| TR01 | Transfer of patent right |