CN103598447B - Non-antibiotic sucking pig milk replacer and preparation method - Google Patents
Non-antibiotic sucking pig milk replacer and preparation method Download PDFInfo
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Abstract
The invention discloses a non-antibiotic sucking pig milk replacer, which comprises various raw materials as follows: puffed corn, puffed soybean, plasma protein powder, steamed fish meal, soybean protein concentrate, low protein whey powder, lactose, fermented soybean meal, glucose, cane sugar, salt, anti-oxidant, lysine, threonine, methionine, tryptophan, zinc oxide, butyrin, microecology preparation, compound acidifying agent, compound enzyme preparation, compound vitamin premix and compound mineral matter premix. The invention employs synergism between butyrin, zinc oxide, acidifying agent and microecology preparation, and in the prerequisite that the antibiotics are not used, the product can help pigling to realize early stage weaning and get rid of weaning stress, and adjust sucking pig gastrointestinal tract health, control diarrhea, increase survival rate, and comprehensively improve immunity and disease resistance of sucking pig.
Description
Technical field
The invention belongs to field of fodder, relate to a kind of piglet feed, particularly a kind of antibiotic-free piglet preweaning feed and preparation method thereof.
Background technology
Animal uses antibiotic to have the history of 1 year, as the medicine for the treatment of, diseases prevention, health care and growth promoting function, serves huge facilitation, still extensive use in present animal husbandry to animal husbandry development.But along with scientific progress and people develop improving constantly of understanding to biology, antibiotic to the harm of the mankind and potential harm also day by day clear and definite.
Abuse of antibiotics can destroy colony balance in animal intestinal; Bacterial drug resistance can be made to strengthen and antibody-resistant bacterium increase; Do not feed antibiotic time animal resistance poor, health normal development is influenced; Cause livestock products medicament residue, residual antibiotic is slowly gathered in human body and other plant by livestock products and environment, finally comes together in human body by various approach.European Union and other developed countries use antibiotic restriction and improve livestock products examination criteria, directly affect the outlet of China's livestock products.Forbid that the cry of abuse of antibiotics resounds through all over the world, also result in the great attention of China, but forbidding antibiotic can bring huge economic loss.China is a developing country, and Animal husbandry production mode family's raising is main, and production environment condition is poor, and the level of production is not high, and strong to antibiotic dependence, thus present stage seeks the extremely urgent thing that other material substitute antibiotics are for we.
Along with the development of domestic pig industry, the Weaning Age of piglet is also constantly shifted to an earlier date.Namely from the wean of average 28 days of past, 21 days are advanced to gradually, even before 21 days.Early weaning piglet not only can shorten sow time of nursing thus improve sow breeding potential and year litter size, improve childbirth house utilization rate, reduce the production cost of piglet, and the propagation of some infectious disease can be blocked.
Early-weaned piglets due to digestive system development immature, digestive ferment system is unsound, gastric acid secretion is not enough, antibody that during wean, parent provides stops and the principal immune system of self is imperfect, on disease with resistivity stress decline and add and factor stress be waited to affect cause that piglet appetite is poor, indigestion, growth inhibition, efficiency of feed utilization are low, diarrhoea even death by physiological environment nutrition.
Publication number is that the Chinese invention patent application of CN1513347 was on July 21st, 2004, which disclose the application of tributyrin as feed addictive, the consumption of tributyrin, according to feed total weight, for 0.1%-0.2%, preferably 0.5%-1.0%.
Although tributyrin uses the function that can play and regulate Gastrointestinal Tract of Weanling Piglets as feed addictive, improve efficiency of feed utilization, but rely on separately tributyrin can not play the effect of substitute antibiotics completely, also reckon without the synergy of other raw material and additive and tributyrin and processing technology to the impact of its function simultaneously.
Summary of the invention
The object of the invention is to use in weanling pig feed in early days easy to digest, easily absorb, without the raw material of ANFs; Simultaneously in order to reduce antibiotic use, in feed, adding some can promote that the functional feedstuff additives such as intestine of young pigs growth, develop immunitypty, raising production performance, control diarrhea carry out substitute antibiotics.
In order to realize foregoing invention object, the invention provides nonreactive piglet preweaning feed, it comprises first time granulation raw material and raw material of granulating for the second time, wherein, described first time granulation raw material comprises the component of following weight portion: popcorn 35-50 part, expanded soybean 15-22 part, steam fish meal 3-5 part, FSPC 2-5 part and fermented bean dregs 1-4 part, described second time granulation raw material comprises the component of following weight portion: SDPP 2-4 part, low albumen whey powder 10-15 part, lactose 5-10 part, glucose 3-5 part, sucrose 2-5 part, salt 0.1-0.3 part, antioxidant 0.05-0.1 part, lysine 0.1-0.3 part, threonine 0.1-0.3 part, methionine 0.1-0.3 part, tryptophan 0.01-0.1 part, zinc oxide 0.1-0.3 part, tributyrin 0.1-0.4 part, probiotics 0.05-0.2 part, compound acidulant 0.1-0.4 part, complex enzyme formulation 0.05-0.2 part, complex vitamin premix 0.1-0.3 part, composite mineral matter premix 0.1-0.3 part.
Wherein, described complex enzyme formulation comprises amylase 1300U/g, protease 2500U/g, cellulase 1500U/g, pectase 2000U/g, phytase 50U/g and 1,4 beta-glucanase 3500U/g.
Wherein, described probiotics comprises bacillus subtilis, lactobacillus acidophilus, Pediococcus acidilactici, Yue Shi lactobacillus and S. cervisiae, total effective viable bacteria content>=1 × 10
9cFU/g.
Wherein, described bacillus subtilis is bacillus subtilis CGMCC No.6511, described lactobacillus acidophilus is lactobacillus acidophilus CGMCC No.6499, described Pediococcus acidilactici is Pediococcus acidilactici CGMCC No.6500, described Yue Shi lactobacillus is Yue Shi lactobacillus CGMCC No.4926, and described S. cervisiae is S. cervisiae No.6560.
Wherein, described acidulant, primarily of fumaric acid, malic acid, citric acid and phosphoric acid composition, adopts vegetable fat bag quilt.
Wherein, described antioxidant is one or more of L-AA, Butylated Hydroxytoluene, fourth hydroxyl methoxy benzene, tocopherol, ethoxyquinoline etc.
Wherein, containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine in the premix of every 1kg composite mineral matter, carrier is stone flour.
Wherein, 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds are contained in every 1kg complex vitamin premix
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
The present invention also provides the preparation method of described nonreactive piglet preweaning feed, and it comprises the steps:
First time granulates: take popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs by weight, by 0.8-1.0 sieve, ultramicro grinding is carried out to each raw material, pulverizing dynamics is 100% mistake 20-40 order, then 150-300 second is mixed, more than 100 DEG C, carry out first time granulate, raw material gelatinization degree reaches more than 90%, obtains semi-finished product;
Second time low temperature is granulated: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in tributyrin again, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, the order of zinc oxide and other raw material carries out the input of small powder, with the semi-finished product mixing 150-300 second of pulverizing by 0.8-1.0 sieve, pulverizing dynamics is 100% mistake 20-40 order, be 0.2-0.4Mpa at steam pressure, pelleting temperature is under the condition of less than 60 DEG C, carry out second time low temperature with 2.5-3.0 aperture ring film to granulate, eventually pass fragmentation, sieve, cooling obtains finished product.
Bacillus subtilis (Bacillus subtilis) in the present invention obtains for applicant is separated from traditional zymotic fermented soya bean, its biological property is as follows: individual cells, 0.7-0.8 × 2-3 μm of bacterium colony rough surface is opaque, dirty white or micro-yellow, bacterium colony is circular, edge indentation, gram-positive bacteria; Gemma 0.6-0.9 × 1.0-1.5 μm, form is oval to column, and be positioned at thalline central authorities or slightly inclined, after sporulation, thalline does not expand, aerobic bacteria.Bacillus subtilis acid resistance in the present invention is strong, bile tolerance, high temperature resistant (can tolerate the pelleting temperature of 80 DEG C), afterwards the biologically active changed can be kept after entering animal intestinal, effective suppression enteropathogenic E. Coli K88, K99 and staphylococcus aureus, promote beneficial bacterium growth.The enzymes such as AMS, protease, lipase, cellulase can be synthesized by thalline self, together play a role with the digestive enzymes in animal body in alimentary canal, feed nutrient digestibility can be improved, improve breeding performonce fo animals, be conducive to reducing culture-cycle and aquaculture cost.The multiple B family vitamin such as energy synthetic vitamin B1, B2, B6, nicotinic acid, for the normal growth of animal body and the performance of physiological function provide safeguard, and improves the activity of interferon and macrophage in animal body, strengthens immunity function and anti-disease ability.Bacillus subtilis of the present invention (Bacillus subtilis) is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 4th, 2012, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6511.
Lactobacillus acidophilus (Lactobacillus acidophilus) in the present invention obtains for applicant is separated from animal intestinal, antibiotin class material (acidolin, bacillus acidophilus's element, lactein) can be secreted, antagonism is produced to pathogenic entero becteria, suppress the propagation of enteron aisle undesirable microorganism, adjustment gut flora balance, its biological property is as follows: bacillus, two terminal circle, 0.6-0.9 × 1.5-6 μm, with single, become two or short chain occurs; Do not move, calot, bacterium colony is coarse.Examine under a microscope, display is wound around or microvillus filiform, stretches out from the center radiation that the bacterium like shadow is piled.Without distinctive pigment.Amarogentin, cellobiose, aesculin, fructose, maltose, galactolipin, lactose, sucrose, mannose, melibiose, raffinose, trehalose can be assimilated, arabinose, gluconate, mannose can not be assimilated.Homofermentation, produces DL-LACTIC ACID.Do not grow at 15 DEG C, optimum growth temperature is 35 DEG C-38 DEG C, and growth initial pH value is 5.0-7.0, and the most suitable growth pH value is 5.5-6.0.Lactobacillus acidophilus of the present invention (Lactobacillus acidophilus) applicant is preserved in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms on September 4th, 2012, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6499.
Pediococcus acidilactici provided by the invention (Pediococcus acidilactici) new strains obtains for applicant is separated from duck jejunal content.Biological property is as follows: cell spherical shape, and two planes alternately division formation tetrad shape at right angle, the life in pairs of general cell, single survivor is rare, does not become catenation.Gram-positive, does not move, amphimicrobian.On MRS culture medium, bacterium colony is little, in white.Glucose, ribose, wood sugar, galactolipin, lactose, sucrose, trehalose, fructose, aesculin, mannose, rhamnose can be utilized, but Arabinose, maltose, sucrose etc. can not be utilized; Not decomposing protein, can not hydrolyzed starch, does not reduce nitrate, does not produce indoles; Catalase reaction negative, negative catalase.Can grow in containing the NaCl environment of 6% ~ 8%, resistance to NaCl concentration 13% ~ 20%.Growth temperature 15-45 DEG C, pH4.5-9.6, pH are that subacidity is good to growing time neutral.Pediococcus acidilactici (Pediococcus acidilactici) new strains HT was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 09 04th, 2012, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6500.
Yue Shi lactobacillus of the present invention (Lactobacillus johnsonii) CGMCCNo.4926 is that applicant screens from the Radix Polygalae Crotalarioidis of Beijing, obtain through the domestication of hydrochloric acid in gastric juice, cholate and seed selection, this strain Yue Shi lactobacillus resistance is stronger, has good acid resistance, artificial gastro-intestinal Fluid tolerance and Bile salt resistance.This bacterial strain is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preservation date is on June 8th, 2011, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, be called for short CGMCC, preserving number is CGMCC No.4926.
Saccharomyces cerevisiae of the present invention (Saccharomy cescerevisiae) strain isolation is in fresh lichee skin, called after K1, and be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center in 2012 09 year 11 days, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6560.Saccharomyces cerevisiae K1 of the present invention is compared with existing saccharomyces cerevisiae, and the Gram-negative common to chitling road and positive bacteria all have inhibitory action, therefore have unexpected technique effect.And this primary yeast is easily cultivated, after fermentation, process, viable count is higher.
Creep feed feed formula of the present invention adopts Ideal Amino Acid Pattern, and net energy system, uses the nutriment of multiple absorption easy to digest, adds synthesizing amino acid and other nutritive additive, meets the nutritional need of weanling pig and improves the physiological function of piggy.
Use FSPC in creep feed of the present invention: FSPC is water-soluble with the removing of high-quality dregs of beans or after alcohol dissolubility non-protein part, obtained containing 65%(butt) soy protein products of above protein.Various amino acid content and abundant, after animal food, digestibility is high, and ANFs is extremely low, and without other peculiar smell, mouthfeel is good.
Fermented bean dregs is used: the soya-bean polypeptides by the enzyme of microorganism secretion in sweat by the partial proteolysis in dregs of beans being molecular weight more than 3000 in creep feed of the present invention.Macro-molecular protein in fermented bean dregs is degraded to polypeptide, little peptide in order, reduces the antigenicity of vegetable protein; Produce a large amount of lactic acid, protease, vitamin etc. in fermentation process, product has genuine milk fragrance or distinctive fermenting aroma; Be rich in lactic acid bacteria, bacillus, the probioticses such as saccharomycete.
Preferably >=99%, zinc oxide has the function of prevention sucking pig diarrhoea and growth promoting effects to the Zn content of zinc oxide of the present invention, and act on effectively stable, be natural prodcuts, have natural attribute, the bacteriostasis of zinc oxide does not find drug resistance so far.
Freshness≤25% of SDPP of the present invention; IGg content >=16%.SDPP main nutrient composition is the required constant of protein, fat, carbohydrate, calcium, phosphorus and some animals and trace quantity mineral substance element, protein content is more than 70%, digestibility is more than 93%, the digestibility of the mineral elements such as iron, phosphorus, magnesium is also higher, can improve the full price of raising poultry nutritive and the balance of daily ration.Containing abundant immune substance in SDPP, immunoglobulin content reaches more than 16% of SDPP, also containing a large amount of immune substances such as growth factor, interferon, lysozyme, these substances are by piglet absorbed intact, effectively can strengthen pigling immunity, improve piglet to the resistance of disease, reduce diarrhea rate and the death rate of piglet.
In the present invention, tributyrin is the energy source material maintaining intestinal epithelial cell, and it can improve intestinal villi growth, reduces Crypt depth; And zinc oxide can by maintaining intestinal epithelial cell permeability and preventing cause of disease material or antigen from avoiding it to the infringement of intestinal epithelial cell to epithelial suction-operated, both synergies can well promote that weanling pig intestinal microecology is healthy, add immunity and nutrient absorption ability that intestinal walls resists pathogen, thus reach the effect of substitute antibiotics, piglet is enable to spend ablactation stress smoothly, avoid having loose bowels, improve immunity and the premunition of sucking pig.
The probiotics added in the present invention contains the profitable strains such as a large amount of lactic acid bacterias and saccharomycete, and the butyric acid that tributyrin decomposition produces and glyceryl 3-monobutyrate all belong to organic acid, with under the synergy of acidulant, regulate the structure of gastrointestinol microorganism flora, promote the flourish of lactic acid bacteria, suppress and stop the breeding of the harmful bacterias such as Escherichia coli, clostruidium, salmonella, β hemolytic bacterioid, increase the quantity of the dominant microfloras such as Bacillus acidi lactici, make beneficial bacterium predominant number.Wherein the synergy of tributyrin and acidulant, probiotics can suppress the breeding of harmful bacteria, makes intestinal flora keep balance, thus reaches the effect of substitute antibiotics.
Adopt the raw materials such as expanded soybean, popcorn, SDPP, steam fish meal, fermented bean dregs simultaneously, while balanced nutritious, improve sucking pig high palatability and digestibility comprehensively, make sucking pig after wean can fast on food.Add a high proportion of lactose, the low albumen whey powder of low albumen, meet the needs of sucking pig to lactose.Low albumen whey powder can provide a large amount of lactose, and in piglet digestive, fermentation can produce a large amount of lactic acid, reduces pH value, helps the digestion of breast, and suppress the growth of malignant bacteria, this has major significance to piglet health; The high-quality lactalbumin contained in low albumen whey powder has high digestibility, good amino acid kenel, advantage without ANFs in piggy body; also containing albumin and globulin (haemocyanin); enteron aisle is had equally to the impact in front; particularly immunoglobulin (Ig); to enteron aisle, there is protected effect, can Escherichia coli be resisted.Also containing newborn peroxidating ferment and lactoferrin in low albumen whey powder, there is the function of sterilization and bacterium processed.
Do not using under antibiotic prerequisite, creep feed of the present invention can help piglet to realize early weaning and smooth in ablactation stress, regulates sucking pig gastrointestinal health, controls diarrhoea, improves survival rate, improves immunity and the premunition of sucking pig comprehensively; Employing amino acid balance is theoretical, selects easy to digest, easily absorbs, and without the raw material of ANFs, while meeting the quick growing nutrient needs of sucking pig, improves palatability and the digestibility of sucking pig comprehensively, ensures sucking pig healthy growth.
After creep feed of the present invention is prepared as and first primary raw material is carried out scientific matching, carry out first time and make semi-finished product, pelleting temperature is more than 100 DEG C, make its complete slaking, starch burn degree reaches more than 90%, not only effectively can remove the ANFs in raw material, raw material palatability and digestibility can also be improved.
Second time low temperature is granulated, other raw material of other are functional and trophism is according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throwing in tributyrin again, complex enzyme formulation, probiotics, the order trace finally throwing in sucrose, glucose, zinc oxide and other auxiliary material claims to carry out the input of small powder, and the error that makes to feed intake is accurate to 0.01kg; After mixing with semi-finished product, low temperature is granulated again, and pelleting temperature is less than 60 DEG C.Second time low temperature is granulated and can be reduced the nutritive loss of heat sensitivity raw material, feeding sequence can ensure that this alkaline matter of zinc oxide does not react with other acidic materials generation acid-base neutralizations such as acidulants, each nutritional labeling is made to reach maximized effectiveness, finished product after the granulation of secondary low temperature can reach the instant effect of 3s, and palatability is better.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Prepared by embodiment 1 bacillus subtilis bacterium powder
1, the preparation of bacillus subtilis (Bacillus subtilis) CGMCC No.6511 active bacteria formulation
1) preparation of bacillus subtilis seed liquor: be seeded to by test tube slant preservation of bacteria strain in the test tube that 15mL culture medium is housed, cultivate 16-20h in 37 DEG C of constant-temperature tables, rotating speed is 225rpm, treats that viable count reaches 10
7cFU/mL, be inoculated in by 1% and be equipped with in the 250mL conical flask of 100mL seed culture medium, cultivate 16-20h in 37 DEG C of constant-temperature tables, shaking speed is 225rpm, treats that viable count reaches 10
7cFU/mL, for subsequent use as seed liquor.
Seed culture medium is: glucose, 20.0g/L; Peptone, 10.0g/L; Dusty yeast, 4.0g/L; Sodium chloride, 5g/L; Distilled water adds to 1000mL, pH7.0 ± 0.2.
2) liquid deep layer fermenting is cultivated
5L vertical digester is selected to carry out liquid fermentation and culture.Be 1% by the inoculum concentration of bacillus subtilis seed liquor, liquid amount 20%, after 37 DEG C of aerobic cultivation 20h, measures the viable count in every milliliter of bacterium liquid, treats that viable count reaches 10
8more than CFU/mL, spore forming rate arrives 80%, and warping terminates fermented and cultured (defoamer is vegetable oil, addition 1%, unit: g/L).
Fermentation medium is composed as follows: peptone, 50g/L; Glucose 10g/L; Dusty yeast 17g/L; Compound sugar 3g/L; Distilled water adds to 1000mL, pH7.0 ± 0.2.
3) freeze-dried vaccine powder preparation
By zymotic fluid centrifugal, concentrate, add the production routines such as freeze drying protectant, vacuum freeze drying and the inspection of bacterium powder viable count, finally prepare powdery active bacteria formulation.
4) finished product detection
Get the powdery active bacteria formulation 1g prepared, measure the viable count in every gram of bacterium powder by international NY/T 1461-2007 method, record viable count in bacterium powder and be greater than 10
8cFU/g.
The preparation of embodiment 2 lactobacillus acidophilus bacterium powder
1) preparation of lactobacillus acidophilus CGMCC No.6499 seed liquor: the transfer needle of preservation of bacteria strain lactobacillus acidophilus in glycerine cryopreservation tube is rule in MRS agar medium flat board, 37 DEG C of constant temperature culture 20h.Then by single colony inoculation that flat board grows in the 250mL conical flask that 100mLMRS fluid nutrient medium is housed, 37 DEG C of constant temperature culture 20h.Again cultured bacterium liquid to be rule on MRS culture medium flat plate cultivation, after 37 DEG C of constant temperature culture 20h, by single colony inoculation in the 250mL conical flask that 100mLMRS fluid nutrient medium is housed, 37 DEG C of constant temperature culture 20h.Repetition 3 times will be operated above, complete the activation of lactobacillus acidophilus.
The preparation of lactobacillus acidophilus seed liquor: the lactobacillus acidophilus bacterium liquid activated is added 5L fermentation tank with 5% inoculum concentration.Culture parameters is: temperature, 37 DEG C; Mixing speed, 50rmp; PH value, 6.0 ± 0.5; Incubation time, 24h.The CaCO of 0.5-1% is added in sweat
3, regulate pH 6.2 ~ 6.5; Carry out feed supplement after fermentation 8h, namely add the glucose 200mL of 50%.
By above-mentioned lactobacillus acidophilus liquid seeds liquid with 1% inoculum concentration join in 50L fermentation tank, culture parameters is: temperature, 37 DEG C; Mixing speed, 50rmp; PH value, 6.0 ± 0.5; Incubation time, 20h.The CaCO of 0.5-1% is added in sweat
3, regulate pH 6.2 ~ 6.5; Carry out feed supplement after fermentation 8h, namely add the glucose 1L of 50%.
The MRS Liquid Culture based formulas of cultivating lactobacillus acidophilus used is as follows: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 4.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 2.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2.
MRS agar medium is on the basis of MRS fluid nutrient medium, add agar 15g/L.
50L fermentation tank Multiplying culture lactobacillus acidophilus optimization culture based formulas used is as follows: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 6.0g/L; Glucose, 25.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 3.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2.
3) freeze-dried vaccine powder preparation
By zymotic fluid centrifugal, concentrate, add the production routines such as freeze drying protectant, vacuum freeze drying and the inspection of bacterium powder viable count, finally prepare powdery active bacteria formulation.
4) finished product detection
Get the powdery active bacteria formulation 1g prepared, measure the viable count in every gram of bacterium powder by international GB/T 4789.35-2003 method, and in bacterium powder, viable count is greater than 10
8cFU/g.
The preparation of embodiment 3 Pediococcus acidilactici bacterium powder
1) preparation of Pediococcus acidilactici CGMCC No.6500 seed liquor
Be seeded to by test tube slant preservation of bacteria strain in the anaerobism pipe that 20mL MRS fluid nutrient medium is housed, 37 DEG C of constant-temperatureanaerobic anaerobic cultivate 20h, treat that viable count reaches 10
7cFU/mL, be inoculated in the 250mL conical flask that 100mL MRS fluid nutrient medium is housed by 1%, 37 DEG C of Anaerobic culturel 20h, treat that viable count reaches 10
7cFU/mL(MRS agar medium plate count) more than, for subsequent use as seed liquor.
2) liquid deep layer fermenting is cultivated
By above-mentioned Pediococcus acidilactici liquid seeds liquid with 1% inoculum concentration join in 50L fermentation tank, culture parameters is: temperature, 37 DEG C; Mixing speed, 50rmp; PH value, 6.0 ± 0.5; Incubation time, 20h.The CaCO of fermentation cumulative volume 0.5% is added in sweat
3, regulate pH at 6.2-6.5; Carry out feed supplement after fermentation 8h, namely add the glucose 1L of 50%.
The MRS Liquid Culture based formulas of cultivating Pediococcus acidilactici used is as follows: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 4.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 2.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2.
MRS agar medium is on the basis of MRS fluid nutrient medium, add agar 15g/L.
50L fermentation tank Multiplying culture Pediococcus acidilactici optimization culture based formulas used is as follows: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 6.0g/L; Glucose, 25.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 3.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2.
3) freeze-dried vaccine powder preparation
By zymotic fluid centrifugal, concentrate, add the production routines such as freeze drying protectant (containing 5% trehalose, 15% skimmed milk power, 4% sucrose, 1%D-arabo-ascorbic acid and 2.0% sodium glutamate), vacuum freeze drying and the inspection of bacterium powder viable count, finally prepare powdery active bacteria formulation.
4) finished product detection
Get the powdery active bacteria formulation 1g prepared, measure the viable count in every gram of bacterium powder by international GB/T4789.35-2003 method, and in bacterium powder, viable count is greater than 10
8cFU/g.
The preparation of embodiment 4 Yue Shi lactobacillus CGMCC No.4926 bacterium powder
The preparation of Yue Shi lactobacillus seed liquor: be seeded to by test tube slant preservation of bacteria strain in the anaerobism pipe that 20mL MRS culture medium is housed, 37 DEG C of constant-temperatureanaerobic anaerobic cultivate 20h, treat that viable count reaches 10
7cFU/mL, be inoculated in by 1% and be equipped with in the 250mL conical flask of 100mL seed culture medium, 37 DEG C of Anaerobic culturel 20h, treat that viable count reaches 10
7more than CFU/mL, for subsequent use as seed liquor.
Seed liquor MRS culture medium is: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 4.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 2.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; Distilled water adds to 1000mL, pH6.2 ± 0.2.
Liquid deep layer fermenting is cultivated
100L vertical digester is selected to carry out liquid fermentation and culture.Wherein, the inoculum concentration of Yue Shi lactobacillus seed liquor is 1%, and liquid amount 65% after 37 DEG C of Anaerobic culturel 20h, measures the viable count in every milliliter of bacterium liquid, treats that viable count reaches 10
8more than CFU/mL, warping terminates fermented and cultured.
Fermentation medium is composed as follows: soy peptone 50g, glucose 10g, dusty yeast 17g, compound sugar 3g, and distilled water adds to 1000mL, pH6.2 ± 0.2.
Prepared by freeze-dried vaccine powder
By zymotic fluid centrifugal, concentrate, add the production routines such as freeze drying protectant, vacuum freeze drying and the inspection of bacterium powder viable count, finally prepare powdery active bacteria formulation.Wherein, freeze drying protectant composed as follows: sucrose 15g, sorbierite 15g, skimmed milk power 18g, distilled water adds to 250mL; Bacterium mud (thalline after centrifugal concentrating) is 1: 125 (g/mL) with the adding proportion of freeze drying protectant, and frozen-dried protective rate reaches more than 72%.
Finished product detection
Get the powdery active bacteria formulation 1g prepared, measure the viable count in every gram of bacterium powder by international GB/T4789.35-2003 method, and in bacterium powder, viable count is greater than 10
8cFU/g.
The preparation of embodiment 5 saccharomyces cerevisiae CGMCC No.6560 bacterium powder
1) slat chain conveyor rejuvenation: the method streak inoculation on No. 98 plating mediums saccharomyces cerevisiae slant strains of Storage in refrigerator being adopted streak inoculation, cultivates 46h in 30 DEG C, makes saccharomyces cerevisiae rejuvenation, and form single bacterium colony; Picking list bacterium colony, on fresh No. 98 slant mediums, cultivates 36h ， Fang Ge refrigerator in 30 DEG C for subsequent use;
2) preparation of first order seed: the fresh slant strains of cultured for step 1) saccharomyces cerevisiae is transferred to and is equipped with in the 500mL triangular flask of 200ml98 culture medium, cultivate 12 ~ 16h, rotating speed 150rpm in 30 DEG C, making it be in the logarithm middle and later periods, is first order seed;
3) preparation of secondary seed: by step 2) cultured saccharomyces cerevisiae seed liquor is transferred to and is equipped with in the 2.0L triangular flask of 1.2L98 culture medium, in 30 DEG C of static gas wave refrigerator 12h, is secondary seed solution;
4) preparation of zymotic fluid: secondary seed step 3) prepared is inoculated into according to the inoculum concentration of 10% 15 ~ 16M is housed
3in the fermentation tank of fermentation medium, temperature 30 DEG C, rotating speed 200rpm, tank pressure 0.05Mpa, cultivate 16h stuck fermentation, now viable count is 1.5 × 10
9cfu/ml;
Described fermentation medium is: brown sugar 1%, soy peptone 1%, yeast extract 0.2%, sodium chloride 0.5%, magnesium sulfate 0.01%, dipotassium hydrogen phosphate 0.2%.
5) preparation of S. cervisiae powder: zymotic fluid prepared by step 4), 2000pm is centrifugal, obtains bacterium mud; adding with the weight/volume percent of bacterium mud is the freeze drying protectant of 10 ~ 15%; in-25 DEG C of freeze dryings after mixing, obtain moisture <5%, viable count>=10
9the S. cervisiae powder of cfu/g;
Described freeze drying protectant be skimmed milk power, glycerine, maltodextrin according to: 2:0.5:1 ratio mixes.
Embodiment 6 nonreactive piglet preweaning feed
First time granulates raw material: popcorn 36.4 parts, expanded soybean 22 parts, steam fish meal 5 parts, FSPC 2 parts, fermented bean dregs 3 parts;
Second time is granulated raw material: SDPP 3 parts, low albumen whey powder 13 parts, lactose 8 parts, glucose 2 parts, sucrose 2.5 parts, salt 0.2 part, antioxidant 0.05 part, lysine 0.3 part, threonine 0.3 part, methionine 0.3 part, tryptophan 0.1 part, 0.25 part, zinc oxide, tributyrin 0.35 part, probiotics 0.2 part, compound acidulant 0.3 part of (pig treasured 500, purchased from Shanghai Niu Cuite feed technology Co., Ltd, this product is primarily of fumaric acid, malic acid, citric acid and phosphoric acid composition, adopt vegetable fat bag quilt), complex enzyme formulation 0.15 part (challenge group), complex vitamin premix 0.3 part, composite mineral matter premix 0.3 part.
Probiotics: bacterium powder embodiment 1 ~ 5 prepared mixes by weight 1:1:1:1, effective viable bacteria content>=1 × 10 total in preparation
9cFU/g.
Complex vitamin premix: containing 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds in every 1kg complex vitamin premix
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
Composite mineral matter premix: containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine in every 1kg composite mineral matter premix, carrier is stone flour.
First time granulates: by aforesaid popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs, by 0.8 sieve, each raw material is pulverized, pulverizing dynamics is 100% mistake 40 orders, then 300 seconds are mixed, more than 100 DEG C, carry out first time granulate, obtain semi-finished product, raw material gelatinization degree reaches more than 90%.
Second time low temperature is granulated: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in tributyrin again, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, aforementioned surplus stock is thrown in by the order of zinc oxide and other auxiliary material, the error that makes to feed intake is accurate to 0.01kg, 300 seconds are mixed with the semi-finished product pulverized by 0.9 sieve, pulverizing dynamics is 100% mistake 30 orders, be 0.4Mpa at steam pressure, pelleting temperature is under the condition of less than 60 DEG C, carry out second time low temperature with 3.0 aperture ring films to granulate, eventually pass fragmentation, sieve, cooling obtains finished product.
Embodiment 7 nonreactive piglet preweaning feed
First time granulates raw material: popcorn 45.79 parts, expanded soybean 18 parts, steam fish meal 3 parts, FSPC 3 parts, fermented bean dregs 4 parts;
Second time is granulated raw material: SDPP 2 parts, low albumen whey powder 11 parts, lactose 5 parts, glucose 3.5 parts, sucrose 3 parts, salt 0.1 part, antioxidant 0.1 part, 98 lysines 0.15 part, threonine 0.2 part, methionine 0.2 part, tryptophan 0.01 part, 0.1 part, zinc oxide, tributyrin 0.1 part, probiotics 0.15 part, compound acidulant 0.15 part of (pig treasured 500, purchased from Shanghai Niu Cuite feed technology Co., Ltd, this product is primarily of fumaric acid, malic acid, citric acid and phosphoric acid composition, adopt vegetable fat bag quilt), complex enzyme formulation 0.2 part (challenge group), complex vitamin premix 0.15 part, composite mineral matter premix 0.1 part.
Probiotics: bacterium powder embodiment 1 ~ 5 prepared mixes by weight 1:1:1:1, effective viable bacteria content>=1 × 10 total in preparation
9cFU/g.
Complex vitamin premix: containing 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds in every 1kg complex vitamin premix
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
Composite mineral matter premix: containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine in every 1kg composite mineral matter premix, carrier is stone flour.
First time granulates: by aforesaid popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs, by 1.0 sieves, each raw material is pulverized, pulverizing dynamics is 100% mistake 40 orders, then 300 seconds are mixed, more than 100 DEG C, carry out first time granulate, obtain semi-finished product, raw material gelatinization degree reaches more than 90%.
Second time low temperature is granulated: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in tributyrin again, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, aforementioned surplus stock is thrown in by the order of zinc oxide and other auxiliary material, the error that makes to feed intake is accurate to 0.01kg, 300 seconds are mixed with the semi-finished product pulverized by 0.8 sieve, pulverizing dynamics is 100% mistake 20 orders, be 0.2Mpa at steam pressure, pelleting temperature is under the condition of less than 60 DEG C, carry out second time low temperature with 3.0 aperture ring films to granulate, eventually pass fragmentation, sieve, cooling obtains finished product.
Embodiment 8 nonreactive piglet preweaning feed
First time granulates raw material: popcorn 39.5 parts, expanded soybean 20.5 parts, steam fish meal 4 parts, FSPC 3 parts, fermented bean dregs 2 parts;
Second time is granulated raw material: SDPP 3 parts, low albumen whey powder 13 parts, lactose 7 parts, glucose 4 parts, sucrose 2 parts, salt 0.1 part, antioxidant 0.1 part, 98 lysines 0.15 part, threonine 0.1 part, methionine 0.2 part, tryptophan 0.05 part, 0.25 part, zinc oxide, tributyrin 0.25 part, probiotics 0.1 part, compound acidulant 0.2 part of (pig treasured 500, purchased from Shanghai Niu Cuite feed technology Co., Ltd, this product is primarily of fumaric acid, malic acid, citric acid and phosphoric acid composition, adopt vegetable fat bag quilt), complex enzyme formulation 0.1 part (challenge group), complex vitamin premix 0.2 part, composite mineral matter premix 0.2 part.
Probiotics: bacterium powder embodiment 1 ~ 5 prepared mixes by weight 1:1:1:1, effective viable bacteria content>=1 × 10 total in preparation
9cFU/g.
Complex vitamin premix: containing 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds in every 1kg complex vitamin premix
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
Composite mineral matter premix: containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine in every 1kg composite mineral matter premix, carrier is stone flour.
First time granulates: by aforesaid popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs, by 0.8 sieve, each raw material is pulverized, pulverizing dynamics is 100% mistake 20 orders, then 300 seconds are mixed, more than 100 DEG C, carry out first time granulate, obtain semi-finished product, raw material gelatinization degree reaches more than 90%.
Second time low temperature is granulated: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in tributyrin again, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, aforementioned surplus stock is thrown in by the order of zinc oxide and other auxiliary material, the error that makes to feed intake is accurate to 0.01kg, 300 seconds are mixed with the semi-finished product pulverized by 0.8 sieve, pulverizing dynamics is 100% mistake 20 orders, be 0.2Mpa at steam pressure, pelleting temperature is under the condition of less than 60 DEG C, carry out second time low temperature with 2.5 aperture ring films to granulate, eventually pass fragmentation, sieve, cooling obtains finished product.
Test example 1
The present invention is applicable to the high-grade piglet preweaning feed of nonreactive of early weaned piglets, through assay, the guarantee value of its nutritional labeling is crude protein >=18, coarse ash≤8.0, crude fibre≤4.0, calcium 0.3-1.3, total phosphorus >=0.3, lysine >=1.5, moisture≤14.0, salt 0.3-1.3.
Test example 2
This test selects the sodium selenite of 160 wean in 28 days as experimental animal, observes the high-grade piglet preweaning feed of nonreactive to the impact of weaned piglets after feeding two weeks.160 weanling pigs are divided into four groups by random, often organize four repetition, namely control group, test 1 group, test 2 groups and test 3 groups.Wherein control group fed tradition is containing antibiotic creep feed daily ration, and test example 1 group, test example 2 groups and test example 3 groups are fed the nonreactive creep feed daily ration in above-described embodiment 6, embodiment 7 and embodiment 8 respectively, and other experimental condition is all consistent.Time-division on-test another name gets the original body mass of every pig, and pig free choice feeding every day and drinking-water, after feeding two weeks, then take the end body weight of every pig respectively according to experimental design.The feed intake of pig often organized every day in duration of test record, simultaneously the survival rate of observed and recorded weanling pig and diarrhea rate.Carry out data analysis with SAS9.0, result all represents with average ± standard deviation.
Average daily ingestion amount (AFI)=feed intake/test number of days
| Control group | Test 1 group | Test 2 groups | Test 3 groups | P | |
| Original body mass (kg) | 6.83±1.36 | 6.45±0.23 | 6.57±1.74 | 6.12±1.45 | P=0.08 |
| Terminate body weight (kg) | 11.03±0.18 | 11.21±2.12 | 11.64±0.85 | 12.27±1.62 | P=0.12 |
| Average daily ingestion amount (g) | 394±45 | 416±60 | 434±40 | 490±33 | P=0.03 |
| Average daily gain (g) | 300±32 | 340±34 | 362±21 | 439±37 | P=0.02 |
| Feedstuff-meat ratio | 1.31±0.04 | 1.22±0.10 | 1.20±0.08 | 1.12±0.07 | P=0.02 |
| Survival rate (%) | 97.5% | 97.5% | 100% | 100% | |
| Diarrhea rate (%) | 12.5% | 7.5% | 5% | 2.5% |
Result of the test is as follows:
Test 1 group, test the average daily ingestion amount of sucking pig and average daily gain in 2 groups and test 3 groups and be all significantly higher than control group (p < 0.05); Wherein test 3 groups and be significantly higher than test 1 group and test 2 groups (p < 0.05).Test 1 group, test 2 groups and test 3 groups in sucking pig feedstuff-meat ratio all work lower than control group (p < 0.05); Wherein test 3 groups significantly lower than test example 1 group and test example 2 groups (p < 0.05).Test the survival rate of sucking pig in 2 groups and test 3 groups higher than control group; Test 1 group, test the diarrhea rate of sucking pig in 2 groups and test 3 groups lower than control group.
Interpretation of result: we can find out test 1 group of searching for food, test the production performance of nonreactive religion groove daily ration piglet in 2 groups and test 3 groups and will be significantly higher than tradition of searching for food and have anti-group from result of the test; And the production performance testing 3 groups of piglets is best, illustrate that the formula of embodiment 8 is optimum formula combination.
Test example 3
This test by system of being granulated by secondary low temperature the high-grade piglet preweaning feed of nonreactive with through tradition once granulation mode the high-grade piglet preweaning feed of nonreactive carry out contrast test.90 28 age in days weanling pigs are divided into two groups by random, often organize three repetitions, i.e. once granulation group and secondary granulation group.Once granulation group is fed and is adopted the high-grade piglet preweaning feed of nonreactive of traditional disposable granulating process processing, and secondary granulation group is fed and adopted the high-grade piglet preweaning feed of nonreactive of secondary low temperature granulating process processing.Test method is with test example 2, and its result is as follows:
| Once granulation group | Secondary granulation group | P | |
| Original body mass (kg) | 6.67±1.24 | 6.54±0.13 | P=0.11 |
| Terminate body weight (kg) | 11.12±2.31 | 12.43±1.42 | P=0.09 |
| Average daily ingestion amount (g) | 410±36 | 484±46 | P=0.01 |
| Average daily gain (g) | 318±41 | 421±50 | P=0.02 |
| Feedstuff-meat ratio | 1.29±0.08 | 1.15±0.07 | P=0.21 |
| Survival rate (%) | 97.5% | 100% | |
| Diarrhea rate (%) | 6.7% | 2.2% |
Result of the test is as follows:
Average daily ingestion amount and the weight average that increases day by day of secondary granulation group sucking pig are significantly greater than once granulation group (p < 0.05).The feedstuff-meat ratio of the sucking pig of secondary granulation group is wanted significantly lower than once granulation group (p < 0.05).The survival rate of secondary granulation group sucking pig be higher than once granulation group and diarrhea rate lower than once granulation group.
Interpretation of result: adopt the granulate high-grade sucking pig material of nonreactive of processing of secondary low temperature to have better production performance than adopting the tradition high-grade piglet preweaning feed of the nonreactive of processing of once granulating, daily gain and survival rate higher, and feedstuff-meat ratio and diarrhea rate lower.Because adopt the processing of secondary low temperature granulating process feedstuff gelatinization rate can be made to reach more than 90% while can also ensure to reduce the nutritive loss of heat sensitivity raw material, each nutritional labeling is made to reach maximized effectiveness, palatability is better, trophism and digestibility higher, sucking pig feed intake is large, therefore, it is possible to reach best feeding effect.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. a nonreactive piglet preweaning feed, is characterized in that: it comprises first time granulation raw material and raw material of granulating for the second time, and wherein, described first time granulation raw material comprises the component of following weight portion: popcorn 35-50 part, expanded soybean 15-22 part, steam fish meal 3-5 part, FSPC 2-5 part and fermented bean dregs 1-4 part, described second time granulation raw material comprises the component of following weight portion: SDPP 2-4 part, low albumen whey powder 10-15 part, lactose 5-10 part, glucose 3-5 part, sucrose 2-5 part, salt 0.1-0.3 part, antioxidant 0.05-0.1 part, lysine 0.1-0.3 part, threonine 0.1-0.3 part, methionine 0.1-0.3 part, tryptophan 0.01-0.1 part, zinc oxide 0.1-0.3 part, tributyrin 0.1-0.4 part, probiotics 0.05-0.2 part, compound acidulant 0.1-0.4 part, complex enzyme formulation 0.05-0.2 part, complex vitamin premix 0.1-0.3 part, composite mineral matter premix 0.1-0.3 part, wherein, described probiotics comprises bacillus subtilis, lactobacillus acidophilus, Pediococcus acidilactici, Yue Shi lactobacillus and S. cervisiae, total effective viable bacteria content>=1 × 10
9cFU/g, described bacillus subtilis is bacillus subtilis CGMCC No. 6511, described lactobacillus acidophilus is lactobacillus acidophilus CGMCC No. 6499, described Pediococcus acidilactici is Pediococcus acidilactici CGMCC No. 6500, described Yue Shi lactobacillus is Yue Shi lactobacillus CGMCC No. 4926, and described S. cervisiae is S. cervisiae No. 6560.
2. nonreactive piglet preweaning feed according to claim 1, is characterized in that: described complex enzyme formulation comprises amylase 1300U/g, protease 2500 U/g, cellulase 1500 U/g, pectase 2000 U/g, phytase 50 U/g and 1,4 beta-glucanase 3500 U/g.
3. nonreactive piglet preweaning feed according to claim 1, is characterized in that: described compound acidulant, primarily of fumaric acid, malic acid, citric acid and phosphoric acid composition, adopts vegetable fat bag quilt.
4. nonreactive piglet preweaning feed according to claim 1, is characterized in that: described antioxidant is one or more in L-AA, Butylated Hydroxytoluene, fourth hydroxyl methoxy benzene, tocopherol, ethoxyquinoline.
5. nonreactive piglet preweaning feed according to claim 1, is characterized in that: containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine in every 1kg composite mineral matter premix, carrier is stone flour.
6. nonreactive piglet preweaning feed according to claim 1, is characterized in that: containing 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds in every 1kg complex vitamin premix
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
7. the preparation method of the nonreactive piglet preweaning feed described in any one of claim 1 ~ 6, it comprises the steps:
First time granulates: take popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs by weight, by 0.8-1.0 sieve, ultramicro grinding is carried out to each raw material, grinding particle size is 100% mistake 20-40 order, then 150-300 second is mixed, more than 100 DEG C, carry out first time granulate, raw material gelatinization degree reaches more than 90%, obtains semi-finished product;
Second time low temperature is granulated: according to first throwing compound acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in tributyrin again, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, the order of zinc oxide and other raw material carries out the input of small powder, with the semi-finished product mixing 150-300 second of pulverizing by 0.8-1.0 sieve, grinding particle size is 100% mistake 20-40 order, be 0.2-0.4MPa at steam pressure, pelleting temperature is under the condition of less than 60 DEG C, carry out second time low temperature with 2.5-3.0 aperture ring film to granulate, eventually pass fragmentation, sieve, cooling obtains finished product.
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